AU2020223370B2 - Enzymes with RuvC domains - Google Patents

Enzymes with RuvC domains Download PDF

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Publication number
AU2020223370B2
AU2020223370B2 AU2020223370A AU2020223370A AU2020223370B2 AU 2020223370 B2 AU2020223370 B2 AU 2020223370B2 AU 2020223370 A AU2020223370 A AU 2020223370A AU 2020223370 A AU2020223370 A AU 2020223370A AU 2020223370 B2 AU2020223370 B2 AU 2020223370B2
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AU
Australia
Prior art keywords
seq
sequence
endonuclease
nos
cases
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AU2020223370A
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AU2020223370A1 (en
Inventor
Lisa ALEXANDER
Christopher Brown
Cristina Butterfield
Audra DEVOTO
Daniela S. A. GOLTSMAN
Rose KANTOR
Jason Liu
Brian Thomas
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Metagenomi Therapeutics Inc
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Metagenomi Inc
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Publication of AU2020223370A1 publication Critical patent/AU2020223370A1/en
Assigned to METAGENOMI, INC. reassignment METAGENOMI, INC. Request for Assignment Assignors: Metagenomi IP Technologies, LLC.
Application granted granted Critical
Publication of AU2020223370B2 publication Critical patent/AU2020223370B2/en
Priority to AU2023206079A priority Critical patent/AU2023206079A1/en
Priority to AU2025279720A priority patent/AU2025279720A1/en
Assigned to Metagenomi Therapeutics, Inc. reassignment Metagenomi Therapeutics, Inc. Request to Amend Deed and Register Assignors: METAGENOMI, INC.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
AU2020223370A 2019-02-14 2020-02-14 Enzymes with RuvC domains Active AU2020223370B2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
AU2023206079A AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains
AU2025279720A AU2025279720A1 (en) 2019-02-14 2025-12-11 Enzymes with RuvC domains

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201962805878P 2019-02-14 2019-02-14
US201962805899P 2019-02-14 2019-02-14
US201962805868P 2019-02-14 2019-02-14
US62/805,868 2019-02-14
US62/805,899 2019-02-14
US62/805,878 2019-02-14
US201962874414P 2019-07-15 2019-07-15
US62/874,414 2019-07-15
PCT/US2020/018432 WO2020168291A1 (en) 2019-02-14 2020-02-14 Enzymes with ruvc domains

Related Child Applications (1)

Application Number Title Priority Date Filing Date
AU2023206079A Division AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains

Publications (2)

Publication Number Publication Date
AU2020223370A1 AU2020223370A1 (en) 2021-10-07
AU2020223370B2 true AU2020223370B2 (en) 2023-04-20

Family

ID=72045639

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2020223370A Active AU2020223370B2 (en) 2019-02-14 2020-02-14 Enzymes with RuvC domains
AU2023206079A Abandoned AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains
AU2025279720A Pending AU2025279720A1 (en) 2019-02-14 2025-12-11 Enzymes with RuvC domains

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2023206079A Abandoned AU2023206079A1 (en) 2019-02-14 2023-07-17 Enzymes with RuvC domains
AU2025279720A Pending AU2025279720A1 (en) 2019-02-14 2025-12-11 Enzymes with RuvC domains

Country Status (9)

Country Link
US (1) US20240117330A1 (enExample)
EP (1) EP3924482A4 (enExample)
JP (3) JP2022520428A (enExample)
KR (2) KR20240007322A (enExample)
CN (2) CN116515797A (enExample)
AU (3) AU2020223370B2 (enExample)
CA (2) CA3241703C (enExample)
MX (2) MX2021009886A (enExample)
WO (1) WO2020168291A1 (enExample)

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WO2021178933A2 (en) 2020-03-06 2021-09-10 Metagenomi Ip Technologies, Llc Class ii, type v crispr systems
EP3204496A1 (en) 2014-10-10 2017-08-16 Editas Medicine, Inc. Compositions and methods for promoting homology directed repair
AU2019291918B2 (en) 2018-06-29 2025-06-12 Editas Medicine, Inc. Synthetic guide molecules, compositions and methods relating thereto
KR20240007322A (ko) * 2019-02-14 2024-01-16 메타지노미, 인크. Ruvc 도메인이 존재하는 효소
US10913941B2 (en) 2019-02-14 2021-02-09 Metagenomi Ip Technologies, Llc Enzymes with RuvC domains
GB2617659B (en) * 2020-02-14 2024-05-01 Metagenomi Inc Enzymes with RuvC domains
CA3177051A1 (en) * 2020-03-31 2021-10-07 Metagenomi, Inc. Class ii, type ii crispr systems
CN116096892A (zh) * 2020-05-08 2023-05-09 宏基因组学公司 具有RuvC结构域的酶
WO2021226369A1 (en) * 2020-05-08 2021-11-11 Metagenomi Ip Technologies, Llc Enzymes with ruvc domains
CA3192224A1 (en) 2020-09-11 2022-03-17 Jyun-Liang LIN Base editing enzymes
GB202418708D0 (en) * 2020-09-11 2025-02-05 Metagenomi Inc Base editing enzymes
JP2023546694A (ja) * 2020-10-21 2023-11-07 エメンドバイオ・インコーポレイテッド 新規のｏｍｎｉ５６、５８、６５、６８、７１、７５、７８及び８４ｃｒｉｓｐｒヌクレアーゼ
EP4240853A2 (en) * 2020-11-03 2023-09-13 Science Solutions LLC Novel class 2 crispr-cas rna-guided endonucleases
CN116964197A (zh) * 2021-01-22 2023-10-27 宏基因组学公司 新型工程化和嵌合核酸酶
WO2022159742A1 (en) * 2021-01-22 2022-07-28 Metagenomi, Inc Novel engineered and chimeric nucleases
KR20230134543A (ko) * 2021-01-22 2023-09-21 메타지노미, 인크. 신규한 조작된 뉴클레아제 및 키메라 뉴클레아제
JP7877351B2 (ja) * 2021-03-19 2026-06-22 メタゲノミ セラピューティクス，インク． Ｃａｓ酵素を用いたマルチプレックス編集
EP4330386A4 (en) * 2021-04-30 2025-05-14 Metagenomi, Inc. Enzymes with ruvc domains
CN119452085A (zh) * 2021-08-27 2025-02-14 宏基因组学公司 具有ruvc结构域的酶
MX2024005505A (es) 2021-11-05 2024-05-23 Metagenomi Inc Enzimas de edicion de bases.
KR20240110603A (ko) 2021-11-24 2024-07-15 메타지노미, 인크. 엔도뉴클레아제 시스템
WO2023122663A2 (en) * 2021-12-23 2023-06-29 Mammoth Biosciences, Inc. Effector proteins and methods of use
WO2023194359A1 (en) * 2022-04-04 2023-10-12 Alia Therapeutics Srl Compositions and methods for treatment of usher syndrome type 2a
KR20250037788A (ko) * 2022-06-15 2025-03-18 빔 테라퓨틱스, 인크. 보체 활성화를 감소시키기 위한 조성물 및 방법
EP4587564A2 (en) * 2022-09-16 2025-07-23 Alia Therapeutics Srl Enqp type ii cas proteins and applications thereof
AU2024230342A1 (en) * 2023-03-01 2025-10-16 Metagenomi Therapeutics, Inc. Enzymes with ruvc domains
WO2025106934A1 (en) * 2023-11-16 2025-05-22 Metagenomi, Inc. Enzymes with ruvc domains

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Also Published As

Publication number Publication date
JP2022520428A (ja) 2022-03-30
CN113728098A (zh) 2021-11-30
KR20240007322A (ko) 2024-01-16
MX2021009886A (es) 2021-10-13
CA3241703A1 (en) 2020-08-20
KR20210139254A (ko) 2021-11-22
AU2020223370A1 (en) 2021-10-07
JP2024133476A (ja) 2024-10-02
CA3130135C (en) 2024-11-19
CA3241703C (en) 2026-02-24
CN116515797A (zh) 2023-08-01
EP3924482A4 (en) 2023-04-05
AU2025279720A1 (en) 2026-01-15
WO2020168291A1 (en) 2020-08-20
AU2023206079A1 (en) 2023-08-10
JP7502537B2 (ja) 2024-06-18
CA3130135A1 (en) 2020-08-20
KR102623312B1 (ko) 2024-01-09
EP3924482A1 (en) 2021-12-22
MX2023006575A (es) 2023-06-16
US20240117330A1 (en) 2024-04-11
JP2023179468A (ja) 2023-12-19

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PC1 Assignment before grant (sect. 113)

Owner name: METAGENOMI, INC.

Free format text: FORMER APPLICANT(S): METAGENOMI IP TECHNOLOGIES, LLC.

FGA Letters patent sealed or granted (standard patent)
HB Alteration of name in register

Owner name: METAGENOMI THERAPEUTICS, INC.

Free format text: FORMER NAME(S): METAGENOMI, INC.