CA3241703C - Enzymes with ruvc domains - Google Patents
Enzymes with ruvc domainsInfo
- Publication number
- CA3241703C CA3241703C CA3241703A CA3241703A CA3241703C CA 3241703 C CA3241703 C CA 3241703C CA 3241703 A CA3241703 A CA 3241703A CA 3241703 A CA3241703 A CA 3241703A CA 3241703 C CA3241703 C CA 3241703C
- Authority
- CA
- Canada
- Prior art keywords
- seq
- sequence
- endonuclease
- nos
- cases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/21—Endodeoxyribonucleases producing 5'-phosphomonoesters (3.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/50—Physical structure
- C12N2310/53—Physical structure partially self-complementary or closed
- C12N2310/531—Stem-loop; Hairpin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Crystallography & Structural Chemistry (AREA)
- Mycology (AREA)
- Cell Biology (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3241703A CA3241703C (en) | 2019-02-14 | 2020-02-14 | Enzymes with ruvc domains |
Applications Claiming Priority (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962805899P | 2019-02-14 | 2019-02-14 | |
| US201962805868P | 2019-02-14 | 2019-02-14 | |
| US201962805878P | 2019-02-14 | 2019-02-14 | |
| US62/805,868 | 2019-02-14 | ||
| US62/805,878 | 2019-02-14 | ||
| US62/805,899 | 2019-02-14 | ||
| US201962874414P | 2019-07-15 | 2019-07-15 | |
| US62/874,414 | 2019-07-15 | ||
| CA3241703A CA3241703C (en) | 2019-02-14 | 2020-02-14 | Enzymes with ruvc domains |
| CA3130135A CA3130135C (en) | 2019-02-14 | 2020-02-14 | Enzymes with RuVC domains |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3130135A Division CA3130135C (en) | 2019-02-14 | 2020-02-14 | Enzymes with RuVC domains |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3241703A1 CA3241703A1 (en) | 2020-08-20 |
| CA3241703C true CA3241703C (en) | 2026-02-24 |
Family
ID=72045639
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3241703A Active CA3241703C (en) | 2019-02-14 | 2020-02-14 | Enzymes with ruvc domains |
| CA3130135A Active CA3130135C (en) | 2019-02-14 | 2020-02-14 | Enzymes with RuVC domains |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3130135A Active CA3130135C (en) | 2019-02-14 | 2020-02-14 | Enzymes with RuVC domains |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20240117330A1 (enExample) |
| EP (1) | EP3924482A4 (enExample) |
| JP (3) | JP2022520428A (enExample) |
| KR (2) | KR20240007322A (enExample) |
| CN (2) | CN116515797A (enExample) |
| AU (3) | AU2020223370B2 (enExample) |
| CA (2) | CA3241703C (enExample) |
| MX (2) | MX2021009886A (enExample) |
| WO (1) | WO2020168291A1 (enExample) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2021178933A2 (en) | 2020-03-06 | 2021-09-10 | Metagenomi Ip Technologies, Llc | Class ii, type v crispr systems |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP3823633A4 (en) | 2018-06-29 | 2023-05-03 | Editas Medicine, Inc. | SYNTHETIC LEAD MOLECULES, COMPOSITIONS AND METHODS RELATED THERETO |
| WO2021226363A1 (en) * | 2020-05-08 | 2021-11-11 | Metagenomi Ip Technologies, Llc | Enzymes with ruvc domains |
| CA3241703C (en) * | 2019-02-14 | 2026-02-24 | Metagenomi Therapeutics, Inc. | Enzymes with ruvc domains |
| US10913941B2 (en) | 2019-02-14 | 2021-02-09 | Metagenomi Ip Technologies, Llc | Enzymes with RuvC domains |
| GB2594339B (en) * | 2020-02-14 | 2023-02-08 | Metagenomi Inc | Enzymes with RUVC domains |
| WO2021202568A1 (en) | 2020-03-31 | 2021-10-07 | Metagenomi Ip Technologies, Llc | Class ii, type ii crispr systems |
| EP4165177A4 (en) * | 2020-05-08 | 2024-08-28 | Metagenomi, Inc. | ENZYMES WITH RUVC DOMAINS |
| JP2023540797A (ja) | 2020-09-11 | 2023-09-26 | メタゲノミ，インコーポレイテッド | 塩基編集酵素 |
| GB2700205B (en) * | 2020-09-11 | 2026-04-15 | Metagenomi Therapeutics Inc | Base editing enzymes |
| WO2022087135A1 (en) * | 2020-10-21 | 2022-04-28 | Emendobio Inc. | Novel omni 56, 58, 65, 68, 71, 75, 78, and 84 crispr nucleases |
| WO2022098681A2 (en) * | 2020-11-03 | 2022-05-12 | Caspr Biotech Corporation | Novel class 2 crispr-cas rna-guided endonucleases |
| CA3205865A1 (en) * | 2021-01-22 | 2022-07-28 | Brian C. Thomas | Novel engineered and chimeric nucleases |
| WO2022159742A1 (en) * | 2021-01-22 | 2022-07-28 | Metagenomi, Inc | Novel engineered and chimeric nucleases |
| JP2024515936A (ja) * | 2021-03-19 | 2024-04-11 | メタゲノミ，インク． | Ｃａｓ酵素を用いたマルチプレックス編集 |
| KR20240004618A (ko) * | 2021-04-30 | 2024-01-11 | 메타지노미, 인크. | Ruvc 도메인을 갖는 효소 |
| AU2022335499A1 (en) | 2021-08-27 | 2024-02-22 | Metagenomi Therapeutics, Inc. | Enzymes with ruvc domains |
| WO2023081855A1 (en) | 2021-11-05 | 2023-05-11 | Metagenomi, Inc. | Base editing enzymes |
| AU2022396533A1 (en) | 2021-11-24 | 2024-05-02 | Metagenomi Therapeutics, Inc. | Endonuclease systems |
| WO2023122663A2 (en) * | 2021-12-23 | 2023-06-29 | Mammoth Biosciences, Inc. | Effector proteins and methods of use |
| WO2023194359A1 (en) * | 2022-04-04 | 2023-10-12 | Alia Therapeutics Srl | Compositions and methods for treatment of usher syndrome type 2a |
| KR20250037788A (ko) * | 2022-06-15 | 2025-03-18 | 빔 테라퓨틱스, 인크. | 보체 활성화를 감소시키기 위한 조성물 및 방법 |
| CA3267752A1 (en) * | 2022-09-16 | 2024-03-21 | Alia Therapeutics Srl | ENQP TYPE II CAS PROTEINS AND THEIR APPLICATIONS |
| AU2024230342A1 (en) * | 2023-03-01 | 2025-10-16 | Metagenomi Therapeutics, Inc. | Enzymes with ruvc domains |
| WO2025106934A1 (en) * | 2023-11-16 | 2025-05-22 | Metagenomi, Inc. | Enzymes with ruvc domains |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113355357B (zh) * | 2012-12-12 | 2024-12-03 | 布罗德研究所有限公司 | 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化 |
| WO2016196655A1 (en) * | 2015-06-03 | 2016-12-08 | The Regents Of The University Of California | Cas9 variants and methods of use thereof |
| WO2016205759A1 (en) * | 2015-06-18 | 2016-12-22 | The Broad Institute Inc. | Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation |
| EP3500967A1 (en) * | 2016-08-17 | 2019-06-26 | The Broad Institute, Inc. | Methods for identifying class 2 crispr-cas systems |
| WO2019165168A1 (en) * | 2018-02-23 | 2019-08-29 | Pioneer Hi-Bred International, Inc. | Novel cas9 orthologs |
| CA3241703C (en) * | 2019-02-14 | 2026-02-24 | Metagenomi Therapeutics, Inc. | Enzymes with ruvc domains |
-
2020
- 2020-02-14 CA CA3241703A patent/CA3241703C/en active Active
- 2020-02-14 KR KR1020247000311A patent/KR20240007322A/ko active Pending
- 2020-02-14 AU AU2020223370A patent/AU2020223370B2/en active Active
- 2020-02-14 CA CA3130135A patent/CA3130135C/en active Active
- 2020-02-14 EP EP20756259.6A patent/EP3924482A4/en active Pending
- 2020-02-14 CN CN202310441977.6A patent/CN116515797A/zh active Pending
- 2020-02-14 JP JP2021547336A patent/JP2022520428A/ja not_active Ceased
- 2020-02-14 WO PCT/US2020/018432 patent/WO2020168291A1/en not_active Ceased
- 2020-02-14 MX MX2021009886A patent/MX2021009886A/es unknown
- 2020-02-14 KR KR1020217028874A patent/KR102623312B1/ko active Active
- 2020-02-14 CN CN202080028787.6A patent/CN113728098A/zh active Pending
-
2021
- 2021-08-16 MX MX2023006575A patent/MX2023006575A/es unknown
-
2023
- 2023-06-15 US US18/335,481 patent/US20240117330A1/en active Pending
- 2023-07-17 AU AU2023206079A patent/AU2023206079A1/en not_active Abandoned
- 2023-09-08 JP JP2023146414A patent/JP7502537B2/ja active Active
-
2024
- 2024-06-05 JP JP2024091759A patent/JP2024133476A/ja active Pending
-
2025
- 2025-12-11 AU AU2025279720A patent/AU2025279720A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP3924482A1 (en) | 2021-12-22 |
| US20240117330A1 (en) | 2024-04-11 |
| CA3130135C (en) | 2024-11-19 |
| KR102623312B1 (ko) | 2024-01-09 |
| CA3130135A1 (en) | 2020-08-20 |
| MX2021009886A (es) | 2021-10-13 |
| MX2023006575A (es) | 2023-06-16 |
| KR20240007322A (ko) | 2024-01-16 |
| CA3241703A1 (en) | 2020-08-20 |
| AU2020223370A1 (en) | 2021-10-07 |
| AU2025279720A1 (en) | 2026-01-15 |
| JP7502537B2 (ja) | 2024-06-18 |
| JP2022520428A (ja) | 2022-03-30 |
| JP2024133476A (ja) | 2024-10-02 |
| JP2023179468A (ja) | 2023-12-19 |
| CN116515797A (zh) | 2023-08-01 |
| AU2020223370B2 (en) | 2023-04-20 |
| EP3924482A4 (en) | 2023-04-05 |
| CN113728098A (zh) | 2021-11-30 |
| WO2020168291A1 (en) | 2020-08-20 |
| KR20210139254A (ko) | 2021-11-22 |
| AU2023206079A1 (en) | 2023-08-10 |
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