JP2022189370A - Composition for enhancing bound water in horny layer - Google Patents

Abstract

To provide a technique capable of enhancing bound water in a horny layer.SOLUTION: The present invention provides a composition for enhancing bound water in a horny layer or a composition for suppressing moisture transpiration in a horny layer, each containing component (A) glycerol and (B) polyglycerol. Suitably, the component (B) is polyglycerol-3. Suitably, the mass ratio of the component (A) content and the component (B) content in the composition is 1: 0.01-0.5.SELECTED DRAWING: None

Description

The present technology relates to a composition for enhancing bound water in the stratum corneum, a composition for suppressing water loss in the stratum corneum, and the like.

The skin is mainly divided into epidermis, dermis and subcutaneous tissue, and the epidermis is further divided into sebaceous membrane, stratum corneum, stratum granulosum, stratum spinosum and stratum basale in order from the surface.

About 80% of the stratum corneum, the outermost layer of the skin, is composed of dead cells composed of keratin protein, and it plays a role of a barrier function that protects the skin from external stimuli such as dryness, ultraviolet rays, and air pollutants. .
The stratum corneum is formed by layers of stratum corneum cells made of hard keratin protein, and is dense enough to prevent the leakage of water and body fluids from the body. It is said that the moisture content of the stratum corneum largely depends on the environmental humidity of the external environment or the moisturizing function of the stratum corneum. It is said that when the water content of the stratum corneum changes, water molecules are mainly stored in keratin fibers and NMF in the stratum corneum cells, which affects the flexibility of the stratum corneum.

In addition, internal factors such as aging, disease, stress, and hormonal abnormalities, and external factors such as dryness and ultraviolet rays, reduce moisture and extracellular matrices (collagen, hyaluronic acid, etc.) from skin tissue, reduce the function of the epidermis and dermis of A decrease in skin function may appear in the form of, for example, skin wrinkles, reduced elasticity (sagging), rough skin, and the like.

As skin care for keeping the skin beautiful, it is common practice to replenish the stratum corneum with moisture and oil. For example, an agent for improving the water retention capacity of the epidermis and/or stratum corneum has been proposed, which contains an apple young fruit extract as an active ingredient.

JP 2020-90465 A

Genyu Imokawa, Yukagaku, 44, 10, 51-66 (1995)

Conventionally, the transpiration of free water in the stratum corneum has been studied in skin care, but the present inventors have focused on bound water in the stratum corneum. However, what kind of ingredients are suitable for further increasing the amount of bound water strongly bound to components such as keratin present in the stratum corneum? The fact is that sufficient knowledge has not yet been obtained.

A main object of the present invention is to provide a technique capable of enhancing bound water in the stratum corneum.

As a result of extensive trial and error, the inventors of the present invention have found that by combining specific components from among polyhydric alcohols, it is possible to achieve almost the same concentration as that of individual components. The present inventors have found that the amount of bound water in the stratum corneum can be synergistically enhanced compared to the amount of bound water in the stratum corneum increased by a single component compared to the control, and the present invention has been completed.

That is, the present invention is as follows.
The present invention provides a composition for enhancing bound water in the stratum corneum or a composition for suppressing water loss in the stratum corneum, containing component (A) glycerin and component (B) polyglycerin.
In addition, the present invention provides an agent for enhancing the binding water in the stratum corneum or water transpiration in the stratum corneum, which is used for blending in a composition containing the component (A) glycerin and the component (B) polyglycerin. Provides an inhibitor.

The component (B) may be polyglycerin-3.
The content mass ratio or use mass ratio of the component (A) and the component (B) may be 1:0.01 to 0.5.
The component (B) may be 5% by mass or less in the composition.
The composition may be a cosmetic or an external skin preparation.
The composition may be used in low humidity environments.

INDUSTRIAL APPLICABILITY The present invention can provide a technique capable of enhancing bound water in the stratum corneum. Note that the effects described here are not necessarily limited, and may be any of the effects described in this specification.

Preferred embodiments for carrying out the present invention are described below. It should be noted that the embodiments described below are examples of representative embodiments of the present invention, and the scope of the present invention should not be construed narrowly. In addition, percentages in this specification are indicated by mass unless otherwise specified. Moreover, the upper limit (or less) and lower limit (or more) of each numerical range (to) can be arbitrarily combined as desired.

1. First embodiment of the present invention This first embodiment is a composition, more preferably a composition for enhancing bound water in the stratum corneum, containing component (A) glycerin and component (B) polyglycerin, Alternatively, a composition for suppressing moisture loss in the stratum corneum can be provided.
The composition of the first embodiment may be for enhancing bound water in the stratum corneum in the epidermis or for suppressing water transpiration, or for enhancing bound water in the epidermis or for suppressing water transpiration. good.
In the first embodiment, the (B) polyglycerin is preferably polyglycerin-3. Also, the composition of the first embodiment is preferably a cosmetic or an external preparation for skin.

1-1. Component (A) Glycerin The component (A) used in the first embodiment is preferably glycerin. Component (A) glycerin is a kind of trihydric alcohol and is also called glycerol ₍ HO--CH.sub.2--CH ₍ OH)--CH.sub.2--OH)). may be used.

1-2. Component (B) Polyglycerin The component (B) used in the first embodiment is preferably polyglycerin. Polyglycerin (for example, triglycerin) is one of polyhydric alcohols and contains multiple glycerin units in the molecule (HO-- ₍ CH.sub.2--CH ₍ OH)--CH.sub.2--O) _.sub.n --H). A commercial product may be used, or one manufactured by a known manufacturing method may be used.
The degree of polymerization of polyglycerin (the number of "poly") is not particularly limited, but is preferably 2 to 20, more preferably 2 to 10, from the viewpoint of enhancing binding water in the stratum corneum and improving suppression of water evaporation. More preferably 2-5, still more preferably 2-4, and more preferably 3. Examples of polyglycerin include polyglycerin-2 (also referred to as diglycerin), polyglycerin-3 (also referred to as triglycerin), polyglycerin-4 (also referred to as tetraglycerin), and polyglycerin-5 (also referred to as pentaglycerin). ) and the like, and one or more selected from these can be used, and among these, polyglycerin-3 (triglycerin) is preferred.

The molecular weight of polyglycerin is not particularly limited, but from the viewpoint of enhancing binding water in the stratum corneum and improving suppression of water evaporation, it is preferably 100 to 2000, more preferably 100 to 800, still more preferably 150 to 400, and further preferably 150 to 400. More preferably 150-300.
The molecular weight can be determined by GPC analysis.

The IOB value of polyglycerol is not particularly limited, but from the viewpoint of enhancing the enhancement of bound water in the stratum corneum and improving the suppression of water evaporation, it is preferably 0.5 to 5.0, more preferably 1.0 to 5.0. , more preferably 2.0 to 4.0, still more preferably 3.0 to 4.0. For example, triglycerin (IOB=3.0), diglycerin (IOB=3.5) and the like can be mentioned, and one or two selected from these are preferred.
The IOB value in the present invention is based on the organic conceptual diagram (Mu Fujita, Prediction of Organic Compounds and Organic Conceptual Diagram, Chemistry Area VOL). 11, No. 10 (1957) 719-715). More specifically, in this organic conceptual diagram, regarding the physicochemical physical properties of a compound, the degree of physical properties mainly due to Van der Waals force is "organic", and the degree of physical properties mainly due to electrical affinity is "organicity". It is a value defined and expressed as "inorganic". The IOB value is an index showing the balance between inorganic and organic properties, and is expressed as IOB value=inorganic value/organic value.

1-3. Content mass ratio (use mass ratio) of component (A) and component (B) in the composition in the first embodiment

The content ratio (mass ratio used) of the component (A) glycerin and the component (B) polyglycerin in the composition is not particularly limited, but from the viewpoint of enhancing the binding water in the stratum corneum and improving the suppression of water transpiration. , When the mass of the component (A) glycerin is 1, the preferred lower limit of the component (B) in the composition is preferably 0.005 or more, more preferably 0.01 or more, and further preferably is 0.02 or more, still more preferably 0.03 or more, more preferably 0.04 or more. It is 0.8 or less, more preferably 0.5 or less, still more preferably 0.4 or less, more preferably 0.3 or less, more preferably 0.2 or less, and more preferably 0.1 or less. Component (A): The component (B) is more preferably 1:0.005 in the composition from the viewpoints of enhancing the enhancement of bound water in the stratum corneum, improving the suppression of water evaporation, and the like, and further from the viewpoint of suppressing stickiness. to 1, more preferably 1:0.01-0.5, even more preferably 1:0.02-0.1.

The content ratio (mass ratio used) of component (A) glycerin and component (B) triglycerin in the composition is not particularly limited, but from the viewpoint of enhancement of bound water in the stratum corneum and improvement of suppression of water transpiration. , when the mass of the component (A) glycerin is 1, the preferable lower limit of the component (B) triglycerin in the composition is preferably 0.005 or more, more preferably 0.01 or more, It is more preferably 0.02 or more, still more preferably 0.03 or more, and more preferably 0.04 or more. , more preferably 0.8 or less, more preferably 0.5 or less, even more preferably 0.4 or less, more preferably 0.3 or less, more preferably 0.2 or less, more preferably 0.1 or less be. Component (A) glycerin: Component (B) triglycerin is more preferably 1: 0.005 to 1, more preferably 1:0.01 to 0.5, still more preferably 1:0.02 to 0.1.

1-4. Total content of component (A) and component (B) in the composition in the first embodiment The total content of component (A) glycerin and component (B) polyglycerin in the composition is particularly limited However, from the viewpoint of enhancing bound water in the stratum corneum and improving suppression of water evaporation, a more preferable lower limit is preferably 0.5% by mass or more, preferably 1% by mass or more, in the composition. It is preferably 2% by mass or more, still more preferably 2.5% by mass or more, more preferably 5% by mass or more, more preferably 8% by mass or more, more preferably 9% by mass or more. As, in the composition, preferably 40% by mass or less, more preferably 30% by mass or less, still more preferably 25% by mass or less, even more preferably 20% by mass or less, more preferably 18% by mass or less, more preferably It is 16% by mass or less, more preferably 15% by mass or less, more preferably 13% by mass or less, and more preferably 12% by mass or less. A more preferable numerical range for the total content of the component (A) and the component (B) in the agent is selected from the viewpoints of enhancement of binding water in the stratum corneum, improvement of suppression of moisture transpiration, and suppression of stickiness. It is more preferably 2.5 to 25% by mass, still more preferably 5 to 15% by mass in the composition.

The total content of component (A) glycerin and component (B) triglycerin in the composition is not particularly limited, but from the viewpoint of enhancing binding water in the stratum corneum and improving moisture transpiration suppression, it is more preferable The lower limit is preferably 0.5% by mass or more, preferably 1% by mass or more, more preferably 2% by mass or more, still more preferably 2.5% by mass or more, more preferably 5% by mass, in the composition. Above, more preferably 8% by mass or more, more preferably 9% by mass or more, and a suitable upper limit is preferably 40% by mass or less, more preferably 30% by mass or less, and even more preferably 30% by mass or less in the composition. is 25% by mass or less, even more preferably 20% by mass or less, more preferably 18% by mass or less, more preferably 16% by mass or less, more preferably 15% by mass or less, more preferably 13% by mass or less, more preferably It is 12% by mass or less. A more preferable numerical range for the total content of component (A) glycerin and component (B) triglycerin in the agent is from the viewpoint of enhancing the enhancement of bound water in the stratum corneum, improving the suppression of moisture loss, and suppressing stickiness. From the point of view, it is more preferably 2.5 to 25% by mass, still more preferably 5 to 15% by mass in the composition.

1-5. Content of component (A) in the composition in the first embodiment

The content of component (A) glycerin in the composition is not particularly limited. Preferably 0.5% by mass or more, preferably 1% by mass or more, more preferably 2% by mass or more, still more preferably 2.5% by mass or more, more preferably 5% by mass or more, more preferably 8% by mass or more , more preferably 9% by mass or more, and as a suitable upper limit, in the composition, preferably 40% by mass or less, more preferably 30% by mass or less, even more preferably 25% by mass or less, and even more preferably is 20% by mass or less, more preferably 18% by mass or less, more preferably 15% by mass or less, more preferably 13% by mass or less, and more preferably 12% by mass or less. A more preferable numerical range for the content of the component (A) glycerin in the agent is more preferable in the composition from the viewpoint of enhancing the enhancement of bound water in the stratum corneum, improving the suppression of moisture evaporation, and the like, and from the viewpoint of suppressing stickiness. It is preferably 2.5 to 25% by mass, more preferably 5 to 15% by mass.

1-6. Content of component (B) in the composition in the first embodiment The content of component (B) polyglycerin in the composition is not particularly limited, but it is From the viewpoint of improvement, etc., the preferred lower limit is preferably 0.05% by mass or more, preferably 0.1% by mass or more, more preferably 0.2% by mass or more, and still more preferably 0 in the composition. .25% by mass or more, more preferably 0.3% by mass or more, more preferably 0.4% by mass or more, and a suitable upper limit is preferably 5% by mass or less, more preferably 5% by mass or less in the composition. is 4% by mass or less, more preferably 3.5% by mass or less, even more preferably 3% by mass or less, more preferably 2.5% by mass or less, more preferably 2% by mass or less, more preferably 1.8% by mass % or less, more preferably 1.5 mass % or less, more preferably 1.3 mass % or less, more preferably 1 mass % or less. A more preferable numerical range for the content of the component (B) in the agent is more preferable in the composition from the viewpoint of enhancing the enhancement of bound water in the stratum corneum, improving the suppression of water evaporation, and the like, and from the viewpoint of suppressing stickiness. is 0.2 to 3.5% by mass, more preferably 0.3 to 1.5% by mass.

The content of the component (B) triglycerin in the composition is not particularly limited, but from the viewpoint of enhancing the enhancement of bound water in the stratum corneum and improving the suppression of water evaporation, the preferred lower limit is Preferably 0.05% by mass or more, preferably 0.1% by mass or more, more preferably 0.2% by mass or more, still more preferably 0.25% by mass or more, more preferably 0.3% by mass or more, and more It is preferably 0.4% by mass or more, and a suitable upper limit is preferably 5% by mass or less, more preferably 4% by mass or less, further preferably 3.5% by mass or less, and further preferably 3.5% by mass or less in the composition. More preferably 3% by mass or less, more preferably 2.5% by mass or less, even more preferably 2% by mass or less, more preferably 1.8% by mass or less, more preferably 1.5% by mass or less, more preferably It is 1.3% by mass or less, more preferably 1% by mass or less. A more preferable numerical range for the content of the component (B) triglycerin in the agent is, from the viewpoint of enhancement of binding water in the stratum corneum, improvement of suppression of water evaporation, etc., and suppression of stickiness in the composition, More preferably 0.2 to 3.5% by mass, still more preferably 0.3 to 1.5% by mass.

1-7. Use in the first embodiment The component (A) and the component (B), or a combination thereof or a mixture thereof, has an effect of enhancing bound water in the stratum corneum or an effect of increasing the amount of bound water in the stratum corneum, an effect of suppressing water evaporation in the stratum corneum, and an effect of suppressing water transpiration by enhancing the bound water, and an action of enhancing the bound water in the stratum corneum or increasing the amount of bound water in the stratum corneum even in a low-humidity environment and/or a change in the humidity environment, and suppressing the transpiration of water in the stratum corneum. action, and a water transpiration suppressing action due to the enhancement of the bound water. In addition, the combination or mixture of the component (A) and the component (B) can exert these various actions in one or two selected from the group consisting of the stratum corneum, the epidermis, and the stratum corneum in the epidermis, In addition, these various actions within the epidermis and/or the stratum corneum can also be exerted. The enhancement of bound water in the stratum corneum means at least that the amount of bound water in the stratum corneum can be increased compared to the amount of bound water in the stratum corneum of the control. For example, as a control, the amount of bound water in the stratum corneum generated when no component is added can be used. By using the component (A) and the component (B) as a better enhancement of the bound water in the stratum corneum, compared to the amount of bound water in the stratum corneum generated when adding the single component, The amount of bound water in can be increased.

Therefore, the present invention provides a method for enhancing binding water in the stratum corneum, suppressing water transpiration in the stratum corneum, and binding the component (A), the component (B), or a combination thereof or a mixture thereof. Various types for use in suppressing water transpiration by enhancing water, enhancing bound water in the stratum corneum in a low humidity environment and/or changing humidity environment, suppressing water transpiration in the stratum corneum, suppressing water transpiration by enhancing the bound water, etc. A composition can be provided. The present invention also provides a composition, more preferably a cosmetic or an external preparation for skin, containing or capable of containing the component (A) and the component (B), or a combination thereof or a mixture thereof. It is possible. The combined use may take the form of a product kit, for example, a product kit comprising a product containing the component (A) and a product containing the component (B).

Further, the component (A) and the component (B), or a combination thereof or a mixture thereof, which are intended to be added, blended or contained in the composition, are an agent for enhancing binding water in the stratum corneum, It can also be provided as an agent of the third embodiment, such as a water transpiration inhibitor, a water transpiration inhibitor by enhancing the bound water, and various agents that can be used even in a low humidity environment and/or a change in humidity environment. . Examples of the composition to which the agent is added include cosmetics and external skin preparations, and the composition of the first embodiment can be obtained by blending the agent of the third embodiment into the composition.

Here, water can be classified into bound water and free water based on the properties of water in the stratum corneum and the mobility and function of water molecules. Free water is water that is weakly adsorbed by components in the stratum corneum and can move around freely, so it is water that easily evaporates as it dries. On the other hand, the bound water in the stratum corneum is water that is strongly bound to components such as keratin in the stratum corneum because it interacts with the hydrophilic portion of coexisting components to restrict its mobility. It is said to be water that is difficult to volatilize even in a state. These free water and bound water can be detected separately by a thermodynamic method such as differential thermal analysis or a spectroscopic method such as near-infrared spectroscopic analysis.

It has been reported that the stratum corneum retains about 33% of water in the form of bound water, and that intercellular lipids of the stratum corneum retain about 13% of the bound water in the stratum corneum (non-patent Reference 1). However, what kind of ingredients are suitable for increasing the amount of bound water that is strongly bound to components in the stratum corneum, such as keratin, and ingredients that can enhance bound water in the stratum corneum. The fact is that sufficient knowledge has not yet been obtained.

However, as a result of intensive studies, the present inventors found that by using a specific combination of the component (A) and the component (B), even if the concentration is almost the same as that of the single component, It was newly found that the amount of bound water in the stratum corneum can be synergistically enhanced compared to the amount of bound water in the stratum corneum. More preferably, even if a small amount of about 0.02 to 0.1 part by mass of component (B) is mixed with 1 part by mass of component (A), the amount of bound water in the stratum corneum synergistically increases. We also found that it can be enhanced. Furthermore, since the bound water in the stratum corneum is water that is difficult to volatilize even in a dry state, the amount of bound water in the stratum corneum is increased by the component (A) and the component (B), thereby improving the present embodiment. If it is in the form, it can be expected to have a better moisturizing effect or skin care effect, prevent or improve skin troubles, maintain a better skin condition, etc. even in a low-humidity environment and/or a change in humidity environment. Further, as shown in [Examples] below, by using a specific combination of the component (A) and the component (B), in the present embodiment, under a low humidity environment and / or under a change in humidity environment It has also been confirmed in a human test that very good water loss suppression results can be obtained in terms of efficacy and its sustainability.

Accordingly, the component (A) and the component (B), or a combination thereof or a mixture thereof, and the composition of the first embodiment prevent, improve, or treat skin troubles; provide skin care; To maintain or improve the skin in a good condition; To moisturize the skin; To specifically improve the moisturizing function; It is possible to prevent, improve, or treat dry skin, which is one of the skin diseases caused by lack of dryness, or to moisturize the skin or maintain the moisture of the skin. can. Skin troubles include dryness of the skin, reduction of moisture in the skin, dryness of the skin, and the like. The skin targeted in this embodiment may be healthy skin or skin with skin disease (sensitive skin, dry skin, etc.).
The component (A) and the component (B), or a combination thereof or a mixture thereof, and the composition of the first embodiment are more preferably used in a low-humidity environment and/or under a change in humidity environment, For example, it can be used for moisturizing, skin care, etc. in a low-humidity environment. etc. is possible. Therefore, the composition of the first embodiment may be a composition for use under a low humidity environment and/or under a change in humidity environment.

By the way, in general, the relative humidity suitable for the skin is 50 to 60% RH. It is said that skin troubles are likely to occur even in healthy skin when the RH is below RH. In general, it is likely to be in a low humidity environment, for example, depending on the season, climatic conditions due to the local environment, and the like. In recent years, the use of equipment and facilities for cooling, heating, air conditioning, ventilation, etc., such as air conditioners, stoves, and fans indoors has made it possible to create a low-humidity environment indoors regardless of the season. Sometimes there are. When the environment is adjusted in this manner, the room temperature is generally set to about 4 to 40.degree. C., 10 to 30.degree. C. or 20 to 30.degree. If the outside air is in a low-humidity environment when the equipment or facilities are used, the inside of the room may be in a lower-humidity environment than the low-humidity outside air.

In addition, depending on the state of wearing the mask and removing it, there may be a local change in the humidity environment only in the part where the mask was worn, and when going out of the bathroom, the humidity may vary from high to low depending on the location or area. Due to the difference in humidity, the humidity environment change may occur on the whole body or on a part of the body. And it is known that under the humidity environment changing from high humidity to low humidity, the moisture content is further reduced and the drying is performed (Reference 3: Sato J et al, Arch Dermatol Res (2001) 293:477). -480 reference). In this way, moisture transpiration from the skin is likely to proceed under changes in the humidity environment, and thus changes in the humidity environment may cause skin troubles and the like.

For this reason, users have demanded cosmetics and topical skin preparations that can prevent the above-described skin troubles and provide better skin care even in low-humidity environments and/or changes in humidity. However, the reality is that this demand has not yet been fully achieved. However, in the present embodiment, it can be used satisfactorily even in such a low humidity environment and / or under a change in humidity environment, and the specific combination of the component (A) and the component (B) in the present embodiment The configuration and the effect thereof were a completely unexpected new discovery even for the inventors of the present invention.

The low-humidity environment in the present embodiment is preferably an environment with a relative humidity lower than 50 to 60% RH, and the preferred upper limit of the relative humidity is more preferably 45% RH or less, and further It is preferably 40% RH or less, more preferably 35% RH or less, more preferably 30% RH or less, and a suitable lower limit is preferably 10% RH or more, more preferably 15% RH or more, and still more preferably is 20% RH or more, and a more suitable range in which the effects of the present embodiment can be exhibited is preferably 5 to 40% RH, more preferably 10 to 35% RH. Under such a low-humidity environment, it is easy to obtain the above-described effects expected by the user when using the present embodiment.

In addition, when evaluating the effect of this embodiment in a low humidity environment, according to <Evaluation method of water transpiration suppression effect in low humidity environment> of <Test Examples 10 to 12> of [Example] below, "initial moisture Evaluation can be performed using the "moisture content ratio (%) at each elapsed time to the amount". Controls are preferably uncoated. At this time, it is preferable to drop 50 μL of a 10% by mass aqueous solution of the sample onto the pad part of the patch tester, and to apply the dropped patch tester to the skin of the subject (preferably the inside of the forearm). , 10 to 20 minutes (more preferably 15 minutes). The number of subjects is not particularly limited, but is preferably 3 or more, for example, about 5 to 10. In the case of evaluation in a low-humidity environment, it is preferable that the measurement is started (0 minutes) when the patch tester is peeled off, and the measurement times are 10 minutes, 30 minutes, and 60 minutes after peeling. It is preferable to set the temperature and relative humidity in a low-humidity environment within a predetermined range (for example, ±1 or ±3% RH) so that there is no fluctuation or a small fluctuation difference. Also, the relative humidity (% RH) and/or temperature (for example, 20 to 30° C., 22° C., etc.) in a low humidity environment may be appropriately set.

Moisture content ratio (%) at each time to the initial moisture content = {(Average moisture content at each time) / (Average skin moisture content before sample application)} x 100
“Average moisture content of bare skin before application of sample” is the average value obtained by dividing the total value of “moisture content of bare skin before application of sample” of each subject by all subjects. “Average moisture content at each time” is the average obtained by dividing the sum of the “average moisture content at each time” for each subject by the total of all subjects.

Under a change in humidity environment in the present embodiment includes at least one period under a high humidity environment and one period under a low humidity environment, and is under a change in humidity environment such as from a high humidity environment to a low humidity environment. etc., but not particularly limited. For example, it may be a local humidity environment change on the skin or face caused by putting on and taking off a mask, or a change in humidity environment such as when leaving the bathroom. It may also be a change in the humidity environment of the whole body or a part of the body (for example, the face or scalp) due to a change in humidity or the like. In this embodiment, even in situations where the humidity environment changes in a short period of time due to mask attachment/detachment, movement, changes in airflow, etc., "high humidity environment → low humidity environment" or "low humidity environment → high humidity environment" Even when changes such as "under the humidity environment" occur in combination or repeatedly occur, and even in a low humidity environment during a change in the humidity environment, the effects of the present embodiment as described above expected by the user can be obtained. easy to obtain. In addition, under the humidity environment change in this embodiment, the same or different "one period under high humidity environment" singular or plural, and the same or different "one period under low humidity environment". good. One period under the high humidity environment is not particularly limited, but the preferable lower limit is preferably 1 minute or more, more preferably 10 minutes or more, and still more preferably 30 minutes or more, and the preferable upper limit is 180 minutes or less. It is more preferably 120 minutes or less, 80 minutes or less, or 30 minutes or less. In addition, one period in the low humidity environment is not particularly limited, but the preferable lower limit is preferably 5 minutes or more, more preferably 10 minutes or more, and still more preferably 30 minutes or more, and the preferable upper limit is preferably It is 300 minutes or less, more preferably 260 minutes or less, still more preferably 200 minutes or less, more preferably 180 minutes or less, 120 minutes or less, or 60 minutes or less. At least within this period, the effects of the present embodiment can be exhibited or maintained more satisfactorily.

Also, the difference in humidity environment change (% RH) can be calculated by "relative humidity (high humidity) - relative humidity (low humidity)". Under a change in humidity environment, if the same or different "one period under high humidity environment" singular or plural and the same or different "one period under low humidity environment" singular or plural are included, the adjacent " 1 period under high humidity environment" and "1 period under low humidity environment", and the difference (% RH) in humidity environment change when falling from "high humidity environment" to "low humidity environment" is obtained, The average value obtained based on these may be used as the difference (% RH) of the change in humidity environment (for example, below <Test Examples 10 to 12> <Evaluation method of water transpiration suppression effect under change in humidity environment> <Measurement method> reference). For the "low humidity" in the humidity environment change, the description of "low humidity" in the above "under low humidity environment" can be appropriately adopted. In addition, the "high humidity" in the humidity environment change preferably refers to a humidity higher than the "low humidity" based on the "low humidity", and includes a relative humidity of 50 to 60% RH. It may be

In addition, the difference in humidity environment change (% RH) can be calculated by "relative humidity (high humidity) - relative humidity (low humidity)", and "period under high humidity environment and period under low humidity environment If there are multiple sets of "period", obtain the average value of high humidity from the period under each high humidity environment and the average value of low humidity from the period under each low humidity environment, and calculate the difference between these average values There may be. For the "low humidity" in the humidity environment change, the description of "low humidity" in the above "under low humidity environment" can be appropriately adopted. In addition, the "high humidity" in the humidity environment change preferably refers to a humidity higher than the "low humidity" based on the "low humidity", and includes a relative humidity of 50 to 60% RH. It may be

A preferable lower limit value of the change difference in the humidity environment under the humidity environment change in the present embodiment is preferably 5% RH or more, more preferably 10% RH or more, and still more preferably 15% RH or more. The upper limit is preferably 60% RH or less, more preferably 50% RH or less, still more preferably 40% RH or less, more preferably 30% RH or less, more preferably 25% RH or less, and the difference in humidity environment is preferably 10 to 30% RH, more preferably 15 to 25% RH. It is more preferable that the low humidity setting in the change difference of the humidity environment under the humidity environment change is 30% RH, or the high humidity setting is more preferably 50% RH. Under such a change in the humidity environment, it is easy to obtain the effect expected by the user when using the present embodiment.

In addition, when evaluating the effect of this embodiment under a change in humidity environment, according to <Evaluation method of water transpiration suppression effect in change in humidity environment> in <Test Examples 10 to 12> of [Example] below, "for the initial moisture content Evaluation can be performed using the "moisture content ratio (%) at each elapsed time". Controls are preferably uncoated. At this time, the application of the patch tester, the application time, and the number of test subjects can adopt the description of the above-mentioned "Evaluation of the present embodiment under a low-humidity environment."
When evaluating the effect of the present embodiment under a change in humidity environment, "high humidity environment → low humidity environment", "low humidity environment → high humidity environment", or, for example, "low Humidity environment → high humidity environment → low humidity environment "It is desirable to evaluate by combining or repeating these, and it is preferable to set a predetermined period for each period under each environment, and the Each period is preferably 10 to 240 minutes, more preferably 10 to 200 minutes, the period under high humidity environment is preferably 10 to 120 minutes, and the period under low humidity environment is preferably 1 to 120 minutes. 200 minutes, more preferably 30 to 200 minutes. In addition, measuring the amount of moisture in the skin of a predetermined part under a low-humidity environment included under a change in the humidity environment is effective for the evaporation of water in the stratum corneum under a low-humidity environment under a change in the humidity environment. It is desirable because it is easy to evaluate the binding water enhancement effect. For example, the subject present in the first high humidity environment is present in the first low humidity environment for 5 to 20 minutes (preferably as a acclimation period); Paste it on the subject's skin (preferably the inside of the forearm) for 10 to 20 minutes; period), 60 to 165 minutes in the first high humidity environment (preferably as an acclimation period), 165 to 360 minutes in the second low humidity environment (preferably for an acclimation period of 10 to 20 minutes, then a measurement period of 175 to 185 minutes) to allow the subject to be present; to measure the moisture content of the subject's skin in the area where the sample was in contact with the patch tester; is from 0 minutes to 360 minutes from the start time. As a more suitable specific evaluation procedure, the subject's skin is conditioned for 15 minutes in the first low humidity environment, and the sample is in contact with the skin for 10 to 20 minutes in the first low humidity environment. The moisture content of the skin is measured for 0 to 60 minutes (measurement time is, for example, 0 minutes, 30 minutes, 60 minutes, etc.), then the subject's skin is acclimatized for 100 to 110 minutes in the first high humidity environment, and the second The skin of the subject is acclimated for 10 to 20 minutes in the second low humidity environment, and after acclimation, the skin is kept in the second low humidity environment for 0 to 180 minutes (measurement time is, for example, 0 minutes, 60 minutes, 90 minutes, 180 minutes, etc.). It is more desirable to measure the moisture content of
In addition, in the evaluation under a change in humidity environment in the present invention, the humidity environment may be changed to a low humidity environment after acclimation in a high humidity environment from the time of peeling the patch tester, or the humidity environment may be changed to a low humidity environment from the time of peeling the patch tester. After acclimating in a humid environment, and then further acclimating in a high humidity environment, the humidity environment may be changed to a low humidity environment. Also, evaluation may be performed by setting the high humidity environment to 22° C. and 50% RH and the low humidity environment to 22° C. and 30% RH, and these temperature and humidity conditions may be changed as appropriate.

Further, as another aspect of the first embodiment, a product kit comprising a product containing a composition containing the component (A) and a product containing a composition containing the component (B), the component By using the product containing (A) and the product containing the component (B) simultaneously or separately, the component (A) and the component (B) may be used in combination. In addition, the composition of the first embodiment may be prepared by mixing the component (A) and the component (B) contained in the product kit at the same time or separately, and such preparation is possible. Such product kits may be provided. For example, a practitioner or an end user may mix the component (A) and the component (B) before use and apply the mixture to the skin, or the component (A) and the component (B) may be mixed. ) on the skin at the same time or separately and mixed on the skin to prepare the composition of the first embodiment.

In addition, as another aspect of the first embodiment, the above-described enhancement of bound water in the epidermis and/or stratum corneum, suppression of water transpiration, suppression of water transpiration due to the enhancement of the bound water, under a low-humidity environment and/or or the component (A) and the component (B), a combination thereof, a mixture of the component (A) and the component (B), or Agents containing said component (A) and said component (B), or uses thereof, can be provided and can be contained or blended in various agents or compositions.

In addition, as another aspect of the first embodiment, the component (A) and the component (B), or a combination thereof or a mixture thereof, the composition of the first embodiment is the above-described epidermal and / or It can be used for a method for enhancing bound water in the stratum corneum, a method for suppressing water transpiration, a method for suppressing water transpiration by enhancing the bound water, or as an active ingredient in these methods.

In addition, as another aspect of the first embodiment, the component (A) and the component (B), or the agent of the third embodiment, is the above-described intradermal and/or stratum corneum binding It can be used for the production of various agents or compositions having a water-enhancing action, a water-transpiration-suppressing action, and these actions under a low-humidity environment and/or under a change in humidity environment, and also as described above. , enhancement of bound water in the epidermis and/or stratum corneum, suppression of water transpiration, and enhancement of bound water in the epidermis and/or stratum corneum under a low humidity environment and/or a change in humidity environment, suppression of water transpiration, etc. It can be used for the production of various agents or various compositions for the purpose of use.

Also, this embodiment may be for therapeutic or non-therapeutic use. "Non-therapeutic purpose" is a concept that does not include medical practice (for example, practice by a doctor), that is, treatment of the human body by therapy. Non-therapeutic purposes include, for example, beauty, massage, and the like.

In the present embodiment, "prevention" refers to preventing or delaying the onset of symptoms or diseases in an application subject, or reducing the risk of developing symptoms or diseases in an application subject. In the present technology, "improvement" refers to amelioration or maintenance of a disease, symptom or condition in an application subject; prevention or delay of deterioration; reversal, prevention or delay of progression.

In addition, the present embodiment can be applied to humans and non-human animals (for example, pets, domestic animals, non-human mammals, etc.) as subjects. Among these, humans and pets are preferred, and humans are more preferred.

<Optional component>
In addition, in the agent or composition containing the component (A) and the component (B), the component (A) and the component (B), if necessary, within a range that does not impair the effect of the present technology, Components used in various preparations such as cosmetics, quasi-drugs, and external skin preparations such as pharmaceuticals can be contained or blended as optional components. Examples of such components include preservatives, cell activators, antioxidants, moisturizers, UV inhibitors, solvents (water, alcohols, etc.), oils, surfactants, thickeners, powders, chelating agents, pH Modifiers, emulsifiers, stabilizers, colorants, brighteners, flavoring agents, flavoring agents, excipients, binders, disintegrants, lubricants, diluents, osmotic pressure regulators, perfumes and the like. One or more of these can be appropriately selected and used. Moreover, the form of the agent of the present embodiment is not particularly limited, and may be in any form such as liquid, paste, gel, solid, and powder.

Moreover, the composition of this embodiment can be obtained by a known production method.
Specific examples of the cosmetics and external skin preparations of the present embodiment include, for example, oil-in-water emulsions, water-in-oil emulsions, milky lotions, creams, lotions, pack cosmetics, liquid foundations, beauty essences, Basic cosmetics such as packs, all-in-one gels, sunscreens and cleansers; Makeup cosmetics such as makeup bases, BB creams, foundations, blushes and lipsticks; Hair cosmetics such as hair tonics, hair tonics, shampoos and rinses; It may be in any form such as dispersion, ointment, liquid, aerosol, patch, poultice, and liniment. Cosmetics and external preparations for skin that are expected to have a moisturizing effect, an emollient effect, and the like are preferred.

2. Second embodiment according to the present invention

In the second embodiment, the component (A) and the component (B), or a combination thereof or a mixture thereof, the composition of the first embodiment, etc., are used on the skin, and are used for the skin, the epidermis and / or the stratum corneum. To provide a method for enhancing bound water in the epidermis and/or a method for suppressing water transpiration, and a method for enhancing bound water in the epidermis and/or the stratum corneum even in a low humidity environment and/or a change in humidity environment, and a method for suppressing water transpiration. be able to.
The use is not particularly limited, but is preferably applied, rubbed, massaged, sprayed, or the like on the skin. The application site of the skin is not particularly limited as long as it is the skin, but one or more selected from the whole body, scalp, face, arms, legs, hands and the like is suitable. Also, the user may be a practitioner such as a masseuse, esthetician, or beautician, or may be a general user or an end user.
The mass ratio of the components (A) and (B) used is preferably 1:0.01 to 0.5.
In addition, the amount of the component (A) used or applied to the target is not particularly limited, but when a 10% aqueous solution of the component (A) and the component (B) is prepared and 1 g of the aqueous solution is used or applied, 0.067 to 0.099 g is preferred, 0.070 to 0.090 g is more preferred, and 0.086 to 0.090 g is even more preferred. In addition, the amount of the component (B) used or applied to the target is not particularly limited. 001 to 0.033 g, more preferably 0.010 to 0.030 g, even more preferably 0.010 to 0.014 g.

In the description of the method of the second embodiment, component (A), component (B), each content, each content mass ratio, manufacturing method, each configuration, each method, description of each term, etc. are omitted as appropriate, but the above "1. First embodiment according to the present invention" and the following " 2. Third embodiment according to the present invention” also applies to this second embodiment, and can be adopted as appropriate. The content and the content mass ratio may be used as the usage amount and the usage mass ratio.

3. Third embodiment of the present invention This third embodiment is a binding water enhancer or water transpiration inhibitor used for blending in a composition, and binding under a low humidity environment and/or a change in humidity environment. A water enhancer or water transpiration inhibitor can be provided.
Moreover, the agent of this second embodiment may contain (A) glycerin and (B) polyglycerin. The (B) polyglycerin is preferably polyglycerin-3.
It is preferable that the weight ratio of the component (A) and the component (B) used is 1:0.01 to 0.5 in the agent. It is preferable to add the component (B) in an amount of 5% by mass or less in the composition. It is preferable to add the component (A) and the component (B) in a total amount of 2.5 to 25% by mass in the composition.

In the description of the agent of the third embodiment, the component (A), the component ( B), each content, each content mass ratio, manufacturing method, each configuration, each method, each term will be omitted as appropriate, but the above-mentioned "1. First embodiment according to the present invention" and "2. The description of "the second embodiment according to the present invention" also applies to this third embodiment, and can be adopted as appropriate. The content and the content mass ratio may be used as the usage amount and the usage mass ratio.
Also, a composition containing the agent of the third embodiment, preferably the composition, can provide a cosmetic or an external preparation for skin. The agent of the third embodiment is contained so that the content and content mass ratio of the component (A) and the component (B) in the composition described in "1. First embodiment according to the present invention" Alternatively, it is preferable to blend them.

In addition, the present technology can employ the following configuration.
- [1] A composition comprising component (A) glycerin and component (B) polyglycerin.
The composition is preferably a composition for enhancing bound water in the stratum corneum or a composition for suppressing water loss in the stratum corneum. The composition is more preferably a composition for use in a low-humidity environment and/or a change in humidity environment, and the low humidity may be a relative humidity lower than 50 to 60% RH. A relative humidity of 20 to 40% RH is more preferable, and use at about room temperature (for example, 4° C. to 40° C.) is more preferable.
[2] The composition according to [1] above, wherein the component (B) polyglycerin is polyglycerin-3.
[3] The composition according to [1] or [2] above, wherein the content ratio of the component (A) and the component (B) is 1:0.01 to 0.5 in the composition. thing.
- [4] The composition according to any one of [1] to [3], wherein the composition is preferably a cosmetic or an external preparation for skin.
[5] The composition is used to enhance bound water in the epidermis and/or the stratum corneum, or to suppress moisture loss in the epidermis and/or the stratum corneum, or the like [4] The composition according to .
- [6] The composition according to the above [5], which is used under a low-humidity environment and/or under a change in humidity environment.
[7] Any one of [1] to [6], wherein the content ratio of the component (A) and the component (B) in the composition is 1:0.01 to 0.5 The composition according to 1.
- [8] The composition according to any one of [1] to [7], wherein the component (B) is 5% by mass or less in the composition.
• [9] An agent for increasing bound water in the stratum corneum or an agent for suppressing evaporation of moisture in the stratum corneum, which is used for blending in a composition containing component (A) glycerin and component (B) polyglycerin. A composition containing the agent, and the composition is preferably a cosmetic or an external preparation for skin.
[10] A product kit comprising (i) a product containing a composition containing component (A) glycerin and (ii) a product containing a composition containing component (B) polyglycerin. It is preferable to mix the (i) composition and the (ii) composition and apply it to the skin.
[11] For use in a low-humidity environment and/or a change in humidity environment, more preferably for use in moisturizing or skin care under a low-humidity environment and/or a change in humidity environment, the above [1] The composition according to any one of [9] or the product kit according to [10].
[12] The moisturizing or skin care prevents healthy skin from drying out in a low-humidity environment in order to moisturize the skin or maintain the moisture of the skin in a low-humidity environment and/or a change in humidity environment, or The composition according to any one of the above [1] to [8] or the above [9], which is used for prevention, improvement, or treatment of dry skin under a low-humidity environment to be improved and / or under a change in humidity environment. Product kit as described. The product may be a container for containing the composition, a sheet for impregnating the composition, a packaging film, or the like.

[13] (A) glycerin and (B) polyglycerin used for enhancing bound water in the epidermis and/or the stratum corneum, or for suppressing water loss in the epidermis and/or the stratum corneum. Preferably, said component (A) is glycerin and/or said component (B) is polyglycerin-3. Furthermore, it is more suitable to use under a low humidity environment and/or under a change in humidity environment.
[14] For producing an agent or composition for enhancing bound water in the epidermis and/or the stratum corneum, or for suppressing water loss in the epidermis and/or the stratum corneum,
Component (A) glycerin and component (B) polyglycerin, or uses thereof. The composition is more preferably used as a cosmetic or an external preparation for skin. More preferably, the agent or composition is used under a low-humidity environment and/or under a change in humidity environment.
[15] Use the composition, product kit, or agent according to any one of [1] to [12] above, or component (A) glycerin and component (B) A method for enhancing bound water in the epidermis and/or stratum corneum, or a method for suppressing water loss, using polyglycerin. More preferably, the method is characterized by being used under a low-humidity environment and/or under a changing humidity environment.

Hereinafter, the present technology will be described in further detail based on examples and the like. It should be noted that the embodiments and the like described below are examples of representative embodiments and the like of the present technology, and the scope of the present technology should not be construed narrowly.

<Test Examples 1 to 9>

Table 1 shows the amount of water bound in the stratum corneum (mg) obtained according to each sample of Test Examples 1 to 9 and <Method for measuring the amount of bound water in the stratum corneum> below.

Raw material Glycerin: ECOCEROL (manufactured by ECOGREEN OLEOCHMICALS)
Polyglycerin-3 (triglycerin): PGL-S (manufactured by Sakamoto Yakuhin Kogyo Co., Ltd.). Polyglycerin-3 had a molecular weight of 240 according to the GPC analysis described above and an IOB value of 3.0 according to the method for determining the IOB value described above.
Dipropylene glycol (manufactured by ADEKA)
1,3-butylene glycol (manufactured by Daicel)

The following were used as the stratum corneum sheet.
Human stratum corneum sheet (manufactured by BIOPREDIC International, product number: STR002, product name: human stratum corneum, overview: derived from the abdomen)
As in Test Example 1, a stratum corneum sheet to which no sample was added was used as a control, and this was taken as 100%. When comparing with this control to determine the enhancement of bound water, the stratum corneum was peeled off from human skin. A sheet (for example, a two-dimensional stratum corneum sheet, a three-dimensional stratum corneum sheet, etc.) prepared by culturing a substance by a known method may be used.

<DSC device>
DSC device: DSC7020 (manufactured by Hitachi High-Tech Science)
Sample pan: AL chromate treated simple sealed container Measurement mode: None

<Method for measuring the amount of bound water in the stratum corneum>
A commercially available human stratum corneum sheet was prepared in a size of 1 cm×1 cm to obtain a dry stratum corneum sheet. Each aqueous solution was prepared as in Test Examples 2 to 9, as shown in Table 1, and these were used as solutions for each Test Example. For example, "9.5% glycerin + 0.5% triglycerin-containing aqueous solution" in Test Example 7 is obtained by mixing 9.5 g of glycerin, 0.5 g of triglycerin, and the remaining water to a total amount of 100 g. can be done.
Each aqueous solution of each test example was added to about 80% of a sterilized petri dish (10 cm in diameter), and the stratum corneum sheet was placed in this petri dish, sealed, and immersed for 20 minutes. After immersion, the stratum corneum sheet was placed on a plate, and moisture on the surface of the stratum corneum sheet was wiped off by sandwiching it with one sheet of Kimwipe (manufactured by Nippon Paper Crecia Co., Ltd.) and pressing it with the index finger for 3 seconds.
The wiped-off stratum corneum sheet was placed in a pan (a simple sealed container for aluminum chromate treatment), and the following first DSC measurement and then second DSC measurement were performed using a DSC measurement device and DSC analysis software. The amount of frozen water (mass: mg) in the stratum corneum sheet containing the sample was obtained by the first DSC measurement, and the water in the stratum corneum sheet containing the sample was completely evaporated by the second DSC measurement. The mass (mg) of the stratum corneum sheet in the state was determined. DSC measurements were sequentially performed for each of the samples of Test Examples 1 to 9.
Calculate the amount of frozen water (free water) (mg) from the melting enthalpy in the heating process obtained using a DSC measuring device, and subtract the amount of frozen water (mg) from the amount of all water in the stratum corneum sheet (mg). Thus, the amount of antifreeze water (bound water amount) (mg) was calculated (Reference 1: OKA T et al, Polymer 41(16): 6055-6059 and Reference 2: Imokawa G et al, Exogenous Dermatology 3(2 ):81-98).

<First DSC measurement: measurement for determining the amount of frozen water>
In this test, the free water contained in the stratum corneum sheet is weakly bound to the components of the stratum corneum (mainly stratum corneum cells) and becomes frozen water. mg). Measurement temperature range: temperature setting from -60°C to 25°C after cooling from 25°C to -60°C, DSC measurement at a scanning rate of 5°C/min. The amount of frozen water was obtained, and this was defined as "amount of frozen water (mg)".
Amount of frozen water (mg) = {melting peak area (mJ/mg) x stratum corneum sheet weight after sample treatment (mg)} / standard ΔH of water (mJ/mg)
The amount of frozen water (mg) in the stratum corneum sheet was obtained using DSC analysis software (Standard analysis) based on the melting peak area (melting enthalpy) of water during the heating process, which is the obtained DSC chart. rice field. Standard melting enthalpy of water at this time: 333.1 (mJ/mg). The mass (mg) of the stratum corneum sheet after sample treatment was obtained by immersing each aqueous solution of each test example for 20 minutes and wiping it off from the mass (mg) of the stratum corneum sheet. is a value obtained by subtracting the mass (mg) of

<Second DSC measurement: Method for determining the amount of all water in the stratum corneum sheet>
After completion of the first DSC measurement A, several holes were made in the upper part of the sample pan with a needle, the sample pan was set in the DSC, the temperature range was raised from 25°C to 120°C, and the scanning speed was 5°C/min. The amount of all the water evaporated from the stratum corneum sheet was obtained by measurement, and this was defined as the "amount of water in the stratum corneum (mg)".

<How to calculate non-freezing water by DSC>
In this test, the bound water contained in the stratum corneum sheet strongly binds to the stratum corneum components (stratum corneum cells, intercellular lipids) and the sample substances in Table 1 that permeate the stratum corneum. Since it becomes water, the amount of non-freezing water (mg) was used as the amount of combined water (mg).
(1) Frozen water was determined by the first DSC measurement. Amount of frozen water (mg) = {melting peak area (mJ/mg) × weight of stratum corneum sheet after treatment (mg)}/standard ΔH of water (mJ/mg)
(2) The amount of water in the stratum corneum was determined by the second DSC measurement.
(3) Antifreeze water was obtained as follows. Amount of antifreeze water (mg) = Amount of water in stratum corneum (mg) - Amount of frozen water (mg)
As an example, the amount of frozen water determined by the first DSC measurement is 0.146 mg, and the amount of all water in the stratum corneum determined by the second DSC measurement is 0.371 mg. 371 mg - 0.146 mg = 0.225 mg was taken as the amount of non-freezing water. The amount of all water in the stratum corneum (0.371 mg) obtained by the second DSC measurement was calculated as follows: [mass of the stratum corneum sheet after sample treatment 0.825 mg] - [the temperature rise in the second DSC measurement This is a value calculated from the stratum corneum sheet mass (mg) 0.454 mg] in which all the water has completely evaporated.

<Results>
In each of Test Examples 2 to 9, when the water of Test Example 1 (no sample added) was used as a control, the bound water in the stratum corneum was enhanced. Among them, the aqueous solution containing 9.5% glycerin + 0.5% triglycerin in Test Example 5, which uses the two components of glycerin and triglycerin in combination, is less bound water than the water-only solution in Test Example 1. The enhancement rate was 1.8 times or more, which was a particularly remarkable effect. Furthermore, when comparing Triglycerin-added in Test Example 5 with Triglycerin-free in Test Example 2, the binding water enhancement rate of Test Example 5 was about 1.4 times that of Test Example 2. , had a remarkable effect. That is, it was suggested that by using a mixed aqueous solution in which a small amount of triglycerin is added to 10% glycerin, the amount of antifreeze water is more than twice the amount of glycerin alone.
Since the bound water in the stratum corneum is strongly bound to the stratum corneum, it can be used as non-freezing water. can be done.
Therefore, it was suggested that by using a mixed aqueous solution in which a small amount of triglycerin is added to glycerin, the amount of bound water in the stratum corneum can be enhanced or increased to twice the amount of glycerin alone.

In addition, the aqueous solutions of Test Examples 5, 7 and 9 were almost the same in absence of stickiness as the 10% glycerin-containing aqueous solution of Test Example 2. On the other hand, the 24% glycerin-containing aqueous solution of Test Example 3 was less sticky than the 10% glycerin-containing aqueous solution of Test Example 2, and had a sticky feeling. From Test Example 5, it was found that by using a polyglycerin such as triglycerin in combination with glycerin, there is an extremely excellent effect of increasing bound water in the stratum corneum while maintaining a non-sticky feeling. The absence of stickiness in the present embodiment was evaluated by a panel of experts, when 0.5 mL of the sample was rubbed on the back of the hand, based on the 10% glycerin-containing aqueous solution, and "compared to the 10% glycerin-containing aqueous solution, the stickiness was It was evaluated on a 3-point scale: “Yes, same, no feeling.”
In addition, when performing an absolute evaluation of the absence of sticky feeling in the present embodiment, it can be performed according to Test Method 3 of [Example] described in JP-A-2018-39789: Absence of sticky feeling. .

<Test Examples 10 to 12>
A 10% by mass glycerin-containing aqueous solution was used as the sample in Test Example 11, and a 9.5% by mass glycerin + 0.5% by mass triglycerin aqueous solution was used as the sample in Test Example 12. Evaluation of moisture transpiration suppression effect> and <Evaluation of moisture transpiration suppression effect under changing humidity environment> were performed. In addition, Test Example 10 as a control was left uncoated. The results of these evaluations are shown in Tables 2 and 3.

<Evaluation of water transpiration suppression effect under low humidity environment>
Table 2 shows the ratio (%) of the water content at each time to the initial water content obtained according to each sample of Test Examples 10 to 12 and the following <Method for evaluating water transpiration suppression effect in a low humidity environment>.

<Evaluation method of water transpiration suppression effect in low humidity environment>
Using the uncoated base composition (each sample) of Test Example 10 and each of the basic compositions (each sample) of Test Examples 11 and 12, the water transpiration suppression action in a low humidity environment was confirmed. In a low humidity environment, assuming that the outside air is drier than the relative humidity of 50 to 60% RH and the indoor temperature is controlled by an air conditioner (approximately 20 to 30 ° C), such as in winter in the Kanto region of Japan, 22 The measurement was performed at °C relative humidity of 30% RH. Furthermore, assuming the case of being in the low-humidity environment for a long time, the measurement time was set to 60 minutes as an evaluation for this long time, and 9 Japanese men and women (20s to 40s) were measured. went.

<Measurement method>
After washing the inside of the forearm, which is the measurement site of the subject, with solid soap and acclimatizing for 15 minutes in an environment of 22° C. and 30% RH, the initial moisture content of the bare skin before application of each sample of Test Examples 11 and 12 was measured. was measured. Next, 50 μL of each sample of Test Examples 11 and 12 was dropped onto the pad portion of a patch tester (manufactured by Torii Pharmaceutical Co., Ltd.). Then, the patch tester containing each of Test Examples 11 and 12 was applied to the inner side of the forearm of the subject for 15 minutes, and Test Example 10 was left unattached (unapplied) for 15 minutes. After 15 minutes, the patch tester containing each sample (Test Examples 10 to 12) was peeled off, and 10 minutes, 30 minutes, and 60 minutes after peeling, it was in contact with the pad part of each sample on the inside of the forearm. The skin moisture content of each part (measurement part) was measured using SKICON (manufactured by Yayoi Co., Ltd.: constant pressure sensor probe contact, high frequency conductance conversion method). The moisture transpiration suppression effect was evaluated from the ratio of the moisture content at each time to the initial moisture content.
Here, the ratio (%) of water content at each time to the initial water content was calculated as follows.
Moisture content ratio (%) at each time to the initial moisture content = {(Average moisture content at each time) / (Average skin moisture content before sample application)} x 100
The average water content at each time is the average value obtained by summing the water content of each subject at each time and dividing the total water content at each time by the number of all subjects. The total number of subjects in this test example was nine.
The average moisture content of the bare skin before application of the sample is the average value obtained by summing the moisture content of the bare skin of each subject before application of the sample and dividing it by the total number of subjects. The total number of subjects in this test example was nine.
It should be noted that the total number of subjects is preferably at least 3 or more.

As shown in Table 2: <Evaluation of moisture transpiration suppression effect in a low humidity environment>, in Test Example 10 in which no sample was applied, the change from the initial moisture content remained within ±10% regardless of the passage of time. In contrast, Test Examples 11 and 12 showed higher water content than Test Example 10 at each time. Furthermore, when comparing Test Examples 11 and 12, Test Example 12, which contains a small amount of triglycerin in 10% glycerin, has a higher moisture content relative to the initial value at each time, and even in a low humidity environment, it can be used for a long time. It was suggested that the transpiration of water is suppressed, and it was confirmed that a stronger effect of suppressing water transpiration was exhibited.

<Evaluation of water transpiration suppression effect under changing humidity environment>
In addition, the relationship between humidity change and moisture content has been studied, and it is known that the moisture content decreases (dries) under environmental changes from high humidity to low humidity (Reference 3: Sato J. et al, Arch Dermatol Res (2001) 293:477-480). Therefore, using the samples of Test Examples 10 to 12, Table 3 shows the water content ratio (%) at each time to the initial water content under changing humidity environment, which was obtained according to the following <Method for evaluating water transpiration suppression effect under changing humidity environment>. shown in

<Method for evaluating water transpiration suppression effect under changing humidity environment>
After performing the above-mentioned <evaluation of moisture transpiration suppression effect in a low humidity environment>, for the skin part that was not applied in Test Example 10 and using the basic composition in Test Examples 11 and 12, according to the following <measurement method>, Furthermore, we confirmed the effect of suppressing water evaporation under a change in humidity environment. The change in the humidity environment from high humidity to low humidity is not particularly limited, but it is assumed that you will actually feel dry when you remove the mask from the state you put it on, or when you go out of the bathroom. Then, the measurement was performed in a humidity environment change from 22° C. relative humidity 50% RH to 22° C. relative humidity 30% RH. Furthermore, assuming a change in humidity in real life, the measurement time after the change in humidity was set to 180 minutes, and the measurement was performed on 9 Japanese men and women (20s to 40s) as subjects.

<Measurement method>
In the above-mentioned <Method for evaluating water transpiration suppression effect in a low humidity environment>, after measuring for 60 minutes in a low humidity environment of 22 ° C. relative humidity of 30% RH, continuously 22 ° C., acclimatization for 105 minutes in a 50% RH environment. did After that, when measuring immediately after the humidity change, considering the possibility that the total water content may be measured higher, after acclimating for 15 minutes in an environment of 22 ° C. and 30% RH, 0 minutes after acclimation , After 60 minutes, after 90 minutes, after 180 minutes, the moisture content of the skin in the same part as the measurement site where the moisture content was measured by the above <Method for evaluating moisture transpiration suppression effect in a low humidity environment> It was measured using SKICON (manufactured by Yayoi Co., Ltd.). The moisture transpiration suppression effect was evaluated from the ratio of the moisture content at each time to the initial moisture content. The initial water content was measured by washing the inner side of the forearm, which is the measurement site of the subject, with solid soap and washing it in a 22°C, 30%RH environment. It is the initial moisture content of the bare skin before applying each sample of Test Examples 11 and 12 after acclimating for 15 minutes under the skin.
Here, the water content ratio (%) at each time to the initial water content was calculated by the method described above.
In addition, under the humidity environment change at this time, the first low humidity environment is 30% RH, the first high humidity environment is 50% RH, and the second low humidity environment is 30% RH. 1 period under humidity environment and 1 period under low humidity environment. From 50% RH under the environment - 30% RH under the second low humidity environment, the difference in humidity environment change is 20% RH. In addition, if the second high humidity environment is 60% RH and then the third low humidity environment is 20% RH, the difference from 60% RH in the second high humidity environment - 20% RH in the third low humidity environment " 40% RH", and the average of these is ("20% RH" + "40% RH")/2 to "30% RH". It may be 20 to 40% RH.

As shown in Table 3: <Evaluation of moisture transpiration suppression effect under changing humidity environment>, in Test Example 10 in which no sample was applied, the change from the initial moisture content remained within ±10% regardless of the passage of time. On the other hand, Test Examples 11 and 12 showed a higher water content than Test Example 10 at each time. Furthermore, when comparing Test Examples 11 and 12, Test Example 12, which contains a small amount of triglycerin in 10% glycerin, has a higher moisture content relative to the initial value at each time, and even when the humidity environment changes from high humidity to low humidity. Furthermore, it was confirmed that even if the elapsed period of 3 to 6 hours after application is long, the effect of suppressing moisture loss is more strongly and continuously exerted.

From this, it can be concluded that a component that enhances the binding water in the stratum corneum has a very excellent effect of suppressing water transpiration in the stratum corneum even in a low-humidity environment and/or a change in humidity environment. I was able to confirm that. As a result, components having an effect of enhancing bound water in the stratum corneum, that is, component (A) glycerin and component (B) polyglycerin (preferably polyglycerin-3), a combination thereof, or a mixture thereof can be used in a low-humidity environment. It has excellent moisturizing or skin care action even under low humidity and/or changes in humidity environment, and can better prevent or improve dryness of healthy skin under low humidity environment and/or change in humidity environment. was confirmed.

The present inventors have found that by making such a specific combination of polyhydric alcohols, it is possible to synergistically increase the amount of bound water in the stratum corneum compared to the single components, and at the same time, in a low-humidity environment and/or Alternatively, it is possible to suppress water evaporation even under a change in humidity environment, and even under a low humidity environment and / or under a change in humidity environment, the moisturizing or skin care effect (particularly, prevention or improvement of dryness of healthy skin) is very high. found to be superior to The fact that moisturizing or skin care in a low-humidity environment and/or a change in humidity environment could not be said to be sufficient in the past could be solved by a combination of specific ingredients selected from among polyhydric alcohols. is an unexpected effect.

A prescription example in the present embodiment will be given below, but the present technology is not narrowly limited by this. The composition of each formulation example of the present embodiment can have a better effect of enhancing bound water in the stratum corneum or suppressing water transpiration in the stratum corneum due to the component (A) and the component (B). Also, it can be a composition for use under a low humidity environment and/or under a change in humidity environment.

Formulation Example 1: Emulsion (oil-in-water type) mass%
1. Remaining amount of purified water 2. Glycerin (Component (A)) 5
3. Triglycerin (Component (B)) 1
4. Ethanol 5
5. 1,3-butylene glycol 5
6. Methyl gluceth-10 2
7. Dimethicone kinematic viscosity at 25° C. 100 mm ² /s 5
8. cetyl ethylhexanoate 2
9. Tocopherol 0.02
10. PEG-9 dimethicone 0.3
11. PEG-11 methyl ether dimethicone 0.3
12. (Acrylates/alkyl acrylate (C10-30)) crosspolymer 0.2
13. Na hydroxide 0.06
14. Phenoxyethanol 0.3
15. Hot spring water 1
16. Serine 0.117. Threonine 0.01
18. Hyaluronic acid Na 0.1

(Production method)
A: Uniformly mix components (1) to (6) at room temperature.
B: Components (12) and (13) are added to A and uniformly mixed and stirred.
C: Components (7) to (11) are added to B and uniformly mixed and stirred.
D: Components (14) to (18) were added to C, and mixed and stirred to obtain an emulsion (oil-in-water type).
The content ratio by mass of the component (A) and the component (B) was 1:0.2 in the emulsion. The milky lotion (oil-in-water type) of Formulation Example 1 obtained as described above can increase the amount of bound water and suppress evaporation of water under a low-humidity environment and/or a change in humidity environment.

Formulation Example 2: Cream (oil-in-water type) mass%
1. Remaining amount of purified water 2. Glycerin (Component (A)) 8
3. Triglycerin (Component (B)) 0.2
4. Ethanol 5
5. 1,3-butylene glycol 5
6. Methyl gluceth-10 2
7. macadamia nut fatty acid phytosteryl 3
8. Phytosteryl oleate 1
9. meadowfoam oil 5
10. PEG-10 hydrogenated castor oil 0.5
11. Polysorbate 80 0.1
12. (Acrylates/alkyl acrylate (C10-30)) crosspolymer 0.3
13. Na hydroxide 0.09
14. Phenoxyethanol 0.3
15. Hot spring water 1
16. Serine 0.1
17. Threonine 0.01
18. Hyaluronic acid Na 0.1
19. Silica 0.5

(Production method)
A: Uniformly mix components (1) to (6) at room temperature.
B: Components (12) and (13) are added to A and uniformly mixed and stirred.
C: Components (7) to (11) are added to B and uniformly mixed and stirred.
D: Components (14) to (19) were added to C, mixed and stirred to obtain a cream (oil-in-water type).
The content ratio by mass of the component (A) and the component (B) was 1:0.025 in the cream. The cream (oil-in-water type) of Formulation Example 2 obtained as described above can increase the amount of bound water and suppress evaporation of water under a low-humidity environment and/or a change in humidity environment.

Formulation Example 3: Essence Mass %
1. Remaining amount of purified water 2. Glycerin (Component (A)) 8
3. Triglycerin (Component (B)) 3.2
4. Ethanol 5
5. 1,3-butylene glycol 5
6. PEG/PPG/polybutylene glycol-8/5/3 glycerin 6
7. PG 3 dicaprate
8. Dimethicone (kinematic viscosity 100 mm2/s at 25°C) 5
9. (dimethicone/vinyl dimethicone)/crosspolymer 2
10. PEG-10 hydrogenated castor oil 0.5
11. Polysorbate 80 0.1
12. (Acrylates/alkyl acrylate (C10-30)) crosspolymer 0.2
13. Na hydroxide 0.06
14. Phenoxyethanol 0.3
15. Hot spring water 1
16. Serine 0.1
17. Threonine 0.01
18. Hyaluronic acid Na 0.1
19. Aureobasidium pullulans culture 5

(Production method)
A: Uniformly mix components (1) to (6) at room temperature.
B: Components (12) and (13) are added to A and uniformly mixed and stirred.
C: Components (7) to (11) are added to B and uniformly mixed and stirred.
D: Components (14) to (19) were added to C, and the mixture was stirred to obtain a beauty essence.
The content ratio by mass of the component (A) and the component (B) was 1:0.4 in the beauty essence. The beauty essence obtained as described above can increase the amount of bound water and suppress evaporation of water under a low-humidity environment and/or a change in humidity environment.

Formulation example 4: lotion mass%
1. Purified water remaining 2. Glycerin (Component (A)) 15
3. Triglycerin (Component (B)) 0.75
4. 1,3-butylene glycol 5
5. Ethanol 1
6. PEG-50 hydrogenated castor oil isostearate 0.2
7. Perfume 0.1
8. Cyclohexane-1,4-dicarboxylic acid bisethoxydiglycol 0.1
9. Phenoxyethanol 0.3

(Production method)
A: Uniformly mix components (1) to (4) at room temperature.
B: Components (5) to (9) are uniformly mixed at room temperature.
C: A lotion was obtained by adding B to A and uniformly mixing and stirring.
The content ratio by mass of the component (A) and the component (B) in the lotion was 1:0.05. The lotion obtained as described above can increase the amount of bound water and suppress evaporation of water under a low humidity environment and/or a change in humidity environment.

Claims (7)

A composition for enhancing bound water in the stratum corneum or a composition for suppressing water loss in the stratum corneum, containing component (A) glycerin and component (B) polyglycerin. A composition according to claim 1, wherein said component (B) is polyglycerin-3. 3. The composition according to claim 1, wherein the content ratio of said component (A) and said component (B) is 1:0.01 to 0.5 in said composition. The composition according to any one of claims 1 to 3, wherein said component (B) is 5% by mass or less in said composition. The composition according to any one of claims 1 to 4, wherein the composition is a cosmetic or an external skin preparation. The composition according to any one of claims 1 to 5, which is used in a low humidity environment. An agent for increasing bound water in the stratum corneum or an agent for suppressing water loss in the stratum corneum, which is used for blending in a composition containing component (A) glycerin and component (B) polyglycerin.