JP2022125300A - Oral composition for oral care - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an oral composition that exhibits an excellent action to prevent and/or improve periodontitis.

SOLUTION: An oral composition for oral care contains pine bark extract, and at least one plant material selected from Coffea arabica, Tilia miqueliana, Pueraria hirsuta, barley young leaves and sweet potato young leaves.

SELECTED DRAWING: Figure 1

COPYRIGHT: (C)2022,JPO&INPIT

Description

TECHNICAL FIELD The present invention relates to an oral composition having an effect of preventing and improving periodontal disease.

Periodontal disease is an inflammatory disease caused by bacterial infection, and is a disease in which inflammation is caused in the gums by bacteria in dental plaque (plaque), and if left untreated, teeth may be lost. In addition, as this periodontal disease progresses, it is said that periodontal disease bacteria may be transported to the organs of the whole body through the capillaries in the oral cavity and cause systemic diseases such as diabetes, pneumonia, arteriosclerosis, and myocardial infarction. .

It has been reported that, for example, a composition containing a pine bark extract is effective as an oral composition for preventing/ameliorating such periodontal disease (see Patent Documents 1 and 2).

Although this pine bark extract has an effect of preventing and improving periodontal disease, further improvement of the effect is required.

WO2005/089784 Japanese Patent Publication No. 2001-511153

The present invention has been made in view of such circumstances, and an object of the present invention is to provide an oral composition that exhibits excellent preventive and/or ameliorating effects on periodontal disease.

The present inventors have engaged in research on oral care, and as a result of intensive studies on foods and medicines that more effectively prevent and/or improve periodontal disease in particular, have found that a specific plant material is a pine bark extract for periodontal disease. The present inventors have found that the preventive and ameliorating effects of are enhanced, and have completed the present invention.

That is, the present invention is as follows.
[1] An oral care composition comprising a pine bark extract and at least one plant material selected from coffee tree, linden tree, kudzu flower, young barley leaf and young sweet potato leaf.
[2] An oral care oral composition containing a pine bark extract as an active ingredient, characterized by further containing at least one plant material selected from coffee tree, linden tree, kudzu flower, barley grass and sweet potato grass. Oral composition for oral care.
[3] An oral composition comprising a pine bark extract and at least one plant material selected from coffee trees and linden trees.

The oral composition of the present invention has an excellent preventive and/or ameliorating effect on periodontal disease.

FIG. 2 shows the growth inhibitory effect of the composition of the present invention (pine bark extract+coffee tree) on Porphyromonas gingivalis. FIG. 2 shows the growth inhibitory effect of the composition of the present invention (pine bark extract+linden tree) on Porphyromonas gingivalis. FIG. 2 shows the growth inhibitory effect of the composition of the present invention (pine bark extract+kudzu flower) on Porphyromonas gingivalis. FIG. 2 shows the growth inhibitory effect of the composition of the present invention (pine bark extract+barley young leaves) on Porphyromonas gingivalis. FIG. 2 shows the growth inhibitory effect of the composition of the present invention (pine bark extract+young sweet potato leaves) on Porphyromonas gingivalis.

The oral composition of the present invention is characterized by containing a pine bark extract and at least one plant material (sometimes referred to as an auxiliary material) selected from coffee tree, linden tree, kudzu flower, young barley leaf and young sweet potato leaf. do. The secondary material may be used singly (for example, only coffee tree), or may be used in combination of two or more. When two or more secondary materials are used in combination, it is preferable to combine secondary materials that are highly effective in enhancing the effect of the pine bark extract.

Since the combination of the pine bark extract and the plant material effectively suppresses the growth of Porphyromonas gingivalis, which is a causative agent of periodontal disease, the oral composition of the present invention effectively prevents and/or can be improved. In addition, the oral composition of the present invention can suppress offensive odors produced when Porphyromonas gingivalis metabolizes proteins and amino acids, and can prevent and/or improve halitosis peculiar to periodontal disease.

[Pine bark extract]
The pine bark extract in the oral composition of the present invention contains proanthocyanidins as one of the main ingredients. Proanthocyanidins are a group of compounds consisting of condensation polymers having a degree of polymerization of 2 or more and having flavan-3-ol and/or flavan-3,4-diol as structural units.

Examples of raw materials for the pine bark extract include French maritime pine (Pinus martima), larch, black pine, Japanese red pine, Japanese white pine, Japanese pine, Korean pine, stone pine, Ryukyu pine, Japanese pines, giant pine, white pine, and the like. Among them, French maritime pine is preferable. The obtained extract may be concentrated or dried as necessary to form a liquid, paste, or powder. Moreover, a commercially available pine bark extract can be used, for example, a pine bark extract (Flavangenol (registered trademark)) manufactured by Toyo Shinyaku Co., Ltd. can be used.

Moreover, the pine bark extract in the oral composition of the present invention preferably contains a polycondensation product having a degree of polymerization of 2 or more as proanthocyanidins. In particular, proanthocyanidins containing a large amount of polycondensates with a low degree of polymerization are preferred. Condensates with a low degree of polymerization include, for example, condensation polymers with a degree of polymerization of 2 to 30 (2- to 30-mers), and condensation polymers with a degree of polymerization of 2 to 10 (2- to 10-mers). Condensation polymers (dimers to tetramers) having a degree of polymerization of 2 to 4 are more preferable. In this specification, a polymer with a degree of polymerization of 2 to 4 is referred to as OPC (oligomeric proanthocyanidin). The pine bark extract in the oral composition of the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and even more preferably 40% by mass or more.

A pine bark extract is obtained by extracting pine bark with water or an organic solvent. When water is used, hot water or hot water is preferably used. Organic solvents used for extraction include foods or drugs such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, and hydrous propylene glycol. Organic solvents acceptable for the production of are preferably used. These water and organic solvents may be used alone, or two or more solvents may be used in combination. In particular, water-soluble solvents such as water, ethanol, and hydrous ethanol are preferably used.

[Plant materials (auxiliary materials)]
The plant material (auxiliary material) in the present invention can be a plant material itself, or a processed product such as a pulverized product, a squeezed juice, or an extract thereof. Powders, granules and the like can be mentioned as pulverized products. The squeezed juice or extract may be in liquid form, but may also be used in the form of paste or dry powder. The extract can be obtained by extraction using an appropriate solvent, and examples of solvents that can be used include water-soluble solvents such as water, ethanol, and hydrous ethanol.
Each plant material will be described below.

(Coffee tree)
The "coffee tree" used in the present invention is a plant belonging to the family Rubiaceae and the genus Coffea, such as Coffee arabica, Coffee canephora Pierr., Coffee robusta, and Liberian coffee tree (Coffee liberica) or the like can be used. The part used as the plant material of the present invention is preferably seeds (coffee beans), and although roasted ones may be used, it is more preferable to use unroasted raw seeds.

(linden)
The linden tree is a plant of the linden family (Tiliaceae), and the linden tree used in the present invention includes Tilia platyphyllos Scop., Tilia cordata Mill., Tilia europaea L., and the like. Examples include plants belonging to the linden family and the genus Tilia. A flower is preferable as the part used as the plant material of the present invention.

(Kuzuhana)
As the kudzu flower, the flower of a plant belonging to the genus Kudzu of the family Fabaceae is used. Kudzu is a climbing perennial plant belonging to the genus Kudzu of the family Fabaceae. As kudzu flowers, those collected in any process from buds to fully opened flowers may be used, and those collected in each process may be mixed and used. The type of kudzu is not particularly limited, but examples include Pueraria thomsonii, Pueraria lobata, and Pueraria thunbergiana. is preferred.

(Young barley leaves)
Barley (Hordeum vulgare L.) is native to Central Asia and is an annual or perennial herb belonging to the Poaceae family. The young barley leaves used as the plant material of the present invention are not particularly limited as long as they are commonly available, and any young barley leaves such as two-rowed barley and six-rowed barley may be used. In addition, young barley leaves of any variety may be used. The young barley leaves may contain young barley leaves, and may contain other parts such as stems.

(sweet potato young leaves)
Sweet potato is a plant belonging to the convolvulaceae family (Convolvulaceae) and is generally called sweet potato. The variety of sweet potato is not particularly limited, and examples thereof include varieties such as Suiou, Joy White, Kogane Sengan, Shiroyutaka, Satsuma Mustachi, and Ayamurasaki.
Among them, watermelon with a high polyphenol content is preferable. The young leaves of the sweet potato used as the plant material of the present invention need only contain the young leaves of the sweet potato, and may contain other parts such as stems. In particular, the tips of stems and leaves (young stems and leaves) are preferred, and young stems and leaves retaining yellowish green color are more preferred.

[Oral composition of the present invention]
The oral composition of the present invention includes, for example, pharmaceuticals (including quasi-drugs), food for specified health uses, food with nutrient function claims, food with functional claims, etc., whose efficacy has been approved by a predetermined organization. It can be used as so-called health foods, general foods, food additives, feeds, and the like.

The oral composition of the present invention can be used for oral care, especially for prevention and/or improvement of periodontal disease. Such an oral care composition and oral composition for preventing and/or improving periodontal disease contains a pine resin extract and a plant material, and is used for oral care and preventing and/or improving periodontal disease. , the product is not particularly limited as long as it can be distinguished from other products. For example, the product according to the present invention, which indicates that it is effective for oral care or that it is effective for preventing and/or improving periodontal disease on any of the main body, packaging, instructions, and advertising materials of the product according to the present invention. Included in the scope of rights.

In addition, the oral care composition of the present invention and the oral composition for preventing and/or improving periodontal disease may be packaged with the combination of the components of the present invention (the pine bark extract + the auxiliary material) in an oral cavity. It is not limited to those labeled as active ingredients for care, prevention and/or improvement of periodontal disease. For example, the active ingredient may not be specified. Alternatively, only the pine bark extract may be indicated as an active ingredient. That is, as one form of the oral care oral composition or oral composition for preventing and/or improving periodontal disease of the present invention, pine bark extract is used as an active ingredient, and coffee tree, linden tree, kudzu flower, and barley grass are used. and young leaves of sweet potato.

Specifically, the oral care composition and oral composition for preventing and/or improving periodontal disease of the present invention include pharmaceuticals (including quasi-drugs) and so-called health foods. For so-called foods, for example, foods labeled as having preventive and/or ameliorating effects on periodontal disease, such as "preserving gum health", "suppressing bleeding from gums", and "reducing periodontal pockets". can be exemplified.

Forms of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. shape, gel shape, chewable shape, stick shape, and the like.

The oral composition of the present invention is preferably in the form of tablets, capsules, powders, granules, or liquids that are easy to take, and particularly preferably beverages, chewable tablets, candies, chewing gums, etc., that stay in the oral cavity for a certain period of time. Specifically, supplements that dissolve in the oral cavity, packaged beverages filled in PET bottles, cans, bottles, etc., and beverages that dissolve in water (hot water), milk, fruit juice, green juice, etc. Examples include instant beverages, candy, chewing gum, and the like. These are preferable in that they are easy to drink after a meal and can improve palatability.

The contents of the pine bark extract and the plant material (ingredients of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effects thereof are exhibited.

In general, when the oral composition of the present invention is a drug or supplement, it preferably contains 0.1 to 100% by mass of the component of the present invention in terms of dry mass, and 1 to 85 mass. %, more preferably 5 to 70% by mass.

In order to exhibit the effect of the present invention more effectively, the component of the present invention preferably accounts for 80% or more of the total oral composition of the present invention in terms of dry mass, and 90% or more. is more preferable, more preferably 95% or more, and particularly preferably 100%.

The blending mass ratio of the pine bark extract and the plant material in the oral composition of the present invention is preferably in the range of 1:0.001 to 1:1000, preferably 1:0.01, in terms of dry mass. It is more preferably in the range of ~1:500, more preferably in the range of 1:0.1 to 1:200.

The intake of the oral composition of the present invention is not particularly limited, but from the viewpoint of exhibiting the effects of the present invention more remarkably, the intake of the pine bark extract per day for adults is 0.5 mg/day or more. preferably 1 mg/day or more, and even more preferably 1.5 mg/day or more. The upper limit is, for example, 500 mg/day, preferably 400 mg/day. The oral composition of the present invention is not particularly limited as long as it requires oral care, but about 80% of them are said to suffer from periodontal disease, adults in their 30s and 40s, and elderly people with weakened gums. is preferably taken.

The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example 2 to 3, so that the daily intake is equal to the above-mentioned intake for one day.

The oral composition of the present invention can be produced by a known method by adding ingredients other than the orally acceptable ingredients of the present invention, if necessary. Ingredients other than the ingredients of the present invention include, for example, water-soluble vitamins (vitamin B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P ), oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, iron; sulfur-containing compounds contained in taurine, garlic, etc.; flavanoids such as hesperidin and quercetin; proteins such as collagen; peptides; amino acids; animal oils and fats; vegetable oils and fats;

EXAMPLES The present invention will be described below based on examples.

[Example 1]
(Test method)
Modified GAM broth (Nissui Pharmaceutical Co., Ltd.) and cultured at 37°C.
P. gingivalis was diluted with Dulbecco's phosphate-buffered saline (manufactured by Nacalai Tesque, Inc.) to prepare a turbidity of 0.5 McFarlamd. A McFarland standard (manufactured by Sysmex bioMérieux Co., Ltd.) was used as a turbidity index.

A modified GAM agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was sterilized with high pressure steam and poured into a sterilized petri dish to prepare a plate medium. P. gingivalis prepared at 0.5 McFarland above was uniformly applied to the plate medium.

PBS or DMSO was used to prepare test substances (pine bark extracts and/or auxiliary materials) at predetermined concentrations. 15 μL of such a test substance was dropped on a 6 mm filter paper for antibiotic assay (manufactured by GE Healthcare), and attached to a petri dish coated with the P. gingivalis.

Using Aneropack Kenki, a square jar and a constant temperature incubator, the cells were cultured in an anaerobic environment at 37° C. for 2 to 3 days.

The diameter of the circle of inhibition for each test substance formed in the petri dish was measured and divided by the diameter of the antibiotic assay filter paper (6 mm). The growth inhibitory effect of each test substance was corrected by the value obtained by dividing the diameter of the inhibition circle of isodine provided on each plate by 6 mm (diameter of inhibition circle of test substance -6 mm (mm)/diameter of inhibition circle of isodine - 6 mm (mm)). The growth inhibitory effect of each test substance was calculated as a ratio to the pine bark extract alone (50 mg/mL), and the growth inhibitory effect of each test substance on P. gingivalis was evaluated.

As the test substance, the following commercially available products or preparations were used.
[Pine bark extract]
A pine bark extract (Toyo Shinyaku; flavangenol (registered trademark)), which is a French maritime pine bark extract, was used.

[Secondary material]
For Coffee robusta, commercially available coffee seed (green coffee bean) extract powder was used.
Lime tree (Tilia cordata Mill.) used a commercially available flower extract powder.
Kudzu flower (Pueraria thomsonii) was used as a flower extract powder extracted with hot water (Toyo Shinyaku Co., Ltd.).
As the barley grass, an extract powder obtained by extracting pulverized powder (Toyo Shinyaku Co., Ltd.) with water-containing ethanol was used.
As the young sweet potato leaves, an extract obtained by extracting pulverized powder (Toyo Shinyaku Co., Ltd.) with water-containing ethanol was used.

Figures 1 to 5 show the evaluation results of the growth inhibitory effect of each test substance on P. gingivalis. FVG in the figure means pine bark extract (Flavangenol (registered trademark)).

As shown in Figures 1 to 5, the combination of the pine bark extract and the specific plant material effectively inhibits the growth of P.gingivalis. In particular, coffee tree, linden tree, young barley leaf and young sweet potato had little or no effect when used alone, but when combined with the pine bark extract, their growth inhibitory activity against P. gingivalis was effectively increased. Kudzu flower by itself also had a growth-inhibiting effect, but the effect was enhanced by combining it with the pine bark extract. Therefore, the oral composition of the present invention prevents and/or improves periodontal disease by effectively suppressing the growth of P.gingivalis, and also prevents and/or improves bad breath peculiar to periodontal disease. It can be seen that it is an excellent oral care composition for oral care.

[Example 2] (Production of tablets)
12 mg of pine bark extract
Lime tree extract powder (derived from flower) 200mg
Sucrose fatty acid ester 350mg
Shellac 10mg
2 mg of fragrance
Reduced maltose Remainder
The mixture consisting of was compressed to produce four 300 mg tablets.

[Example 3] (Production of chewable tablet)
Pine bark extract 1.5 mg
Coffea extract powder (derived from green coffee beans) 150mg
Hydroxypropyl cellulose 250mg
Sucrose fatty acid ester 50mg
Aspartame 30mg
Perfume 3 mg
Reduced maltose Remainder
The mixture consisting of was compressed to produce two 500 mg chewable tablets.

[Example 4] (Production of granules)
Pine bark extract 50mg
Barley grass powder 2000mg
Fructooligosaccharide 200mg
Reduced maltose Remainder
After mixing, the mixture consisting of was granulated to produce 3 g of granules.

[Example 5] (Production of powder)
100 mg of pine bark extract
Young sweet potato powder 1500mg
50 mg of tea extract
dextrin residue
A mixture consisting of was mixed to produce 3 g of powder.

[Example 6] (Production of candy)
Pine bark extract 30 mg
Coffea extract powder (derived from green coffee beans) 50mg
Bodhi tree extract powder (derived from flower) 20mg
sweetener (aspartame/L-phenylalanine compound,
acesulfame K, stevia) 30mg
Perfume 10mg
Reduced maltose starch syrup Remainder
A candy of 3 g consisting of was produced according to a conventional method.

[Example 7] (Production of packaged beverage)
200 mg of pine bark extract
Kudzu flower extract 35mg
Black tea leaf extract powder 500mg
Sodium ascorbate 50mg
water
A 350 ml packaged beverage consisting of was produced according to a conventional method.

Since the oral composition of the present invention can be used as a health food or the like having a high effect of preventing and/or improving periodontal disease, the industrial utility of the present invention is high.

Claims (2)

An oral composition comprising a pine bark extract and linden tree. An oral care oral composition comprising a pine bark extract as an active ingredient, further comprising at least one plant material selected from linden tree, young sweet potato and arrowroot.

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