JP2021113230A - Oral composition for oral care - Google Patents

Abstract

To provide an oral composition that exhibits an excellent action to prevent and/or improve periodontitis.SOLUTION: An oral composition for oral care contains pine bark extract, and at least one plant material selected from Coffea arabica, Tilia miqueliana, Pueraria hirsuta, barley young leaves and sweet potato young leaves.SELECTED DRAWING: Figure 1

Description

The present invention relates to an oral composition having a preventive / ameliorating effect on periodontal disease.

Periodontal disease is an inflammatory disease caused by bacterial infection, in which bacteria in plaque cause inflammation of the gums, and if left untreated, there is a risk of losing teeth. It is also said that as this periodontal disease progresses, periodontal disease bacteria may be carried to organs throughout the body through capillaries in the oral cavity, causing systemic diseases such as diabetes, pneumonia, atherosclerosis, and myocardial infarction. ..

As an oral composition for preventing / ameliorating such periodontal disease, for example, a composition containing a pine bark extract has been reported to be effective (see Patent Documents 1 and 2).

Although this pine bark extract has a preventive / ameliorating effect on periodontal disease, further improvement of the effect is required.

WO2005 / 089784 Japanese Patent Application Laid-Open No. 2001-511153

The present invention has been made in view of such circumstances, and an object of the present invention is to provide an oral composition having an excellent preventive and / or ameliorating effect on periodontal disease.

As a result of working on oral care research and studying foods and pharmaceuticals that prevent and / or improve periodontal disease more effectively, the present inventors have found that a specific plant material is periodontal disease of pine bark extract. We have found that the preventive / ameliorating effect of the present invention is enhanced, and have completed the present invention.

That is, the present invention is as follows.
[1] An oral composition for oral care, which comprises a pine bark extract and at least one plant material selected from coffee tree, linden tree, kudzu flower, barley young leaf and sweet potato young leaf.
[2] An oral composition for oral care containing a pine bark extract as an active ingredient is characterized by further containing at least one plant material selected from coffee tree, linden tree, kudzu flower, barley young leaves and sweet potato young leaves. Oral composition for oral care.
[3] An oral composition comprising a pine bark extract and at least one plant material selected from coffee trees and linden trees.

The oral composition of the present invention has an excellent preventive and / or ameliorating effect on periodontal disease.

It is a figure which shows the growth inhibitory effect on Porphyromonas gingivalis of the composition of this invention (pine bark extract + coffee tree). It is a figure which shows the growth inhibitory effect on Porphyromonas gingivalis of the composition of this invention (pine bark extract + linden tree). It is a figure which shows the growth inhibitory effect on Porphyromonas gingivalis of the composition of this invention (pine bark extract + kudzu flower). It is a figure which shows the growth inhibitory effect on Porphyromonas gingivalis of the composition of this invention (pine bark extract + barley young leaf). It is a figure which shows the growth inhibitory effect on Porphyromonas gingivalis of the composition of this invention (pine bark extract + sweet potato young leaf).

The oral composition of the present invention is characterized by containing a pine bark extract and at least one plant material (sometimes referred to as a secondary material) selected from coffee tree, linden tree, kudzu flower, barley young leaves and sweet potato young leaves. do. As the auxiliary material, one type may be used alone (for example, only coffee tree), or two or more types may be used in combination. When two or more kinds of auxiliary materials are used in combination, it is preferable to combine auxiliary materials having a high effect of enhancing the effect of the pine bark extract.

Since the combination of the pine bark extract and the plant material effectively suppresses the growth of Porphyromonas gingivalis, which is the causative agent of periodontal disease, the oral composition of the present invention effectively prevents and / or prevents periodontal disease. Can be improved. In addition, the oral composition of the present invention can suppress bad odor generated when Porphyromonas gingivalis metabolizes proteins and amino acids, and can prevent and / or improve bad breath peculiar to periodontal disease.

[Pine bark extract]
The pine bark extract in the oral composition of the present invention contains proanthocyanidins as one of the main components. Proanthocyanidins are a group of compounds composed of flavan-3-ol and / or flavan-3,4-diol as a constituent unit and having a degree of polymerization of 2 or more.

Examples of the raw material of the pine bark extract include French coastal pine (Pinus Martima), Karamatsu, Black pine, Japanese red pine, Himekomatsu, Pinus parviflora, Pinus parviflora, Pinus pinaster, Pinus pinaster, Pinus pinaster, Japanese red pine, and white pine. Among them, French coastal pine is preferable. The obtained extract may be concentrated or dried as required to be liquid, paste-like, or powder. As the pine bark extract, a commercially available product can be used. For example, a pine bark extract (flavangenol (registered trademark)) manufactured by Toyo Shinyaku Co., Ltd. can be used.

Further, the pine bark extract in the oral composition of the present invention preferably contains a reduced polymer having a degree of polymerization of 2 or more as a proanthocyanidin. In particular, proanthocyanidins containing a large amount of a reduced polymer having a low degree of polymerization are preferable. Examples of the reduced polymer having a low degree of polymerization include a reduced polymer having a degree of polymerization of 2 to 30 (2 to 30 mer) and a reduced polymer having a degree of polymerization of 2 to 10 (2 to 10 mer). Preferably, a condensed polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable. In the present specification, a polymer having a degree of polymerization of 2 to 4 is referred to as OPC (oligomeric procyanidin). The pine bark extract in the oral composition of the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and further preferably 40% by mass or more.

The pine bark extract is obtained by extracting the pine bark with water or an organic solvent. When water is used, hot water or hot water is preferably used. Examples of the organic solvent used for extraction include foods or chemicals such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, and hydrous propylene glycol. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more kinds of solvents. In particular, a water-soluble solvent such as water, ethanol, or hydrous ethanol is preferably used.

[Plant material (secondary material)]
As the plant material (secondary material) in the present invention, in addition to the plant material itself, processed products such as crushed products, juices, and extracts thereof can be used. Examples of the pulverized product include powder, granules and the like. The juice and extract may be liquid, but can also be used as a paste or a dry powder. The extract can be obtained by extracting with an appropriate solvent, and as the solvent, for example, a water-soluble solvent such as water, ethanol, or hydrous ethanol can be used.
Hereinafter, each plant material will be described.

(Coffee tree)
The "coffee tree" used in the present invention is a plant of the Akane family (Rubiaceae) and the genus Coffee tree (Coffea). For example, Arabic coffee tree (Coffee arabica), Robusta coffee tree (Coffee canephora Pierr.), Coffee robusta, Liberia coffee tree (Coffee liberica) etc. can be used. As the part used as the plant material of the present invention, seeds (coffee beans) are preferable, and roasted seeds may be used, but unroasted raw seeds are more preferable.

(linden)
The linden tree is a plant of the linden family (Tiliaceae), and the linden tree used in the present invention includes Tilia platyphyllos Scop., Tilia cordata Mill., Tilia europaea L., and the like. Examples include plants of the family Tilia cordata and the genus Lindens (Tilia). Flowers are preferable as the site used as the plant material of the present invention.

(Kuzuhana)
As the kudzu flower, a flower of a plant belonging to the genus Kudzu of the leguminous family is used. Kuzu is a climbing perennial plant of the genus Pueraria montana in the family Leguminosae. As the kudzu flower, those collected in any process from the bud to the fully opened flower may be used, and those collected in each process may be mixed and used. The type of kudzu is not particularly limited, but Pueraria thomsonii, Pueraria lobata, Pueraria thunbergiana, etc. can be exemplified, and Pueraria thomsonii. Is preferable.

(Wakaba barley)
Barley (Hordeum vulgare L.) is an annual or perennial herb native to Central Asia and belongs to the Gramineae family, and is roughly classified into two-row barley and six-row barley according to the panicle shape. The young barley leaves used as the plant material of the present invention are not particularly limited as long as they are usually available, and any young barley leaves such as two-row barley and six-row barley may be used. Moreover, any kind of young barley leaves may be used. The young barley leaves may contain young leaves of barley and may contain other parts such as stems.

(Sweet potato young leaves)
Sweet potato is a plant belonging to the family Convolvulaceae, and is generally called sweet potato. The varieties of sweet potato are not particularly limited, and examples thereof include varieties such as Satsuma, Joy White, Koganesengan, Shiroyutaka, Satsuma Starch, and Ayamurasaki.
Of these, soybeans having a high polyphenol content are preferable. The young sweet potato leaves used as the plant material of the present invention may contain young leaves of sweet potato, and may contain other parts such as stems. In particular, the tip portion (young foliage) of the foliage is preferable, and the young foliage in a state of maintaining a yellowish green color is more preferable.

[Oral composition of the present invention]
The oral composition of the present invention includes, for example, pharmaceuticals (including non-pharmaceutical products), functional foods whose efficacy has been approved by a predetermined organization such as foods for specified health use, foods with nutritional function, foods with functional claims, etc. It can be used as a so-called health food, general food, food additive, feed and the like.

The oral composition of the present invention can be used for oral care, particularly prevention and / or amelioration of periodontal disease. The oral composition for oral care and the oral composition for prevention and / or improvement of periodontal disease contain a pine resin extract and a plant material, and are used for oral care and prevention and / or improvement of periodontal disease. In the above, there is no particular limitation as long as the product can be distinguished from other products. For example, the main body, packaging, instruction manual, or promotional material of the product according to the present invention indicates that it is effective for oral care and that it is effective in preventing and / or improving periodontal disease. Included in the scope of rights.

In the oral composition for oral care and the oral composition for periodontal disease prevention and / or improvement of the present invention, the combination component (pine bark extract + auxiliary material) of the present invention is used in the packaging of the product or the like. It is not limited to those listed as active ingredients for care and periodontal disease prevention and / or improvement. For example, the active ingredient may not be specified. Further, only the pine bark extract may be displayed as an active ingredient. That is, as one form of the oral composition for oral care or the oral composition for prevention and / or improvement of periodontal disease of the present invention, a pine bark extract is used as an active ingredient, and coffee tree, linden tree, kudzu flower, young barley leaf. And a form containing at least one plant material selected from young sweet potato leaves.

Specifically, examples of the oral composition for oral care and the oral composition for prevention and / or improvement of periodontal disease of the present invention include pharmaceuticals (including quasi-drugs) and so-called health foods. In so-called foods, for example, those labeled as having a preventive and / or ameliorating effect on periodontal diseases such as "maintaining the health of the gums", "suppressing bleeding from the gums", and "shallowing the periodontal pocket". It can be exemplified.

The form of the oral composition of the present invention includes, for example, tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, and caplets. Examples include a shape, a gel shape, a chewable shape, and a stick shape.

The oral composition of the present invention is preferably in the form of tablets, capsules, powders, granules, or liquids that are easy to ingest, and particularly preferably beverages, chewable tablets, candies, chewing gums, etc. that stay in the oral cavity for a certain period of time. Specifically, for supplements that dissolve in the oral cavity, packaged beverages filled in PET bottles, cans, bottles, etc., and for drinking by dissolving in water (hot water), milk, fruit juice, green juice, etc. Examples thereof include instant beverages, candy, and chewing gum. These are preferable because they are easy to drink after meals and can enhance palatability.

The content of the pine bark extract and the plant material (ingredient of the present invention) in the oral composition of the present invention may be appropriately contained within the range in which the effect is exhibited.

Generally, when the oral composition of the present invention is a pharmaceutical product or a supplement, the component of the present invention is preferably contained in an amount of 0.1 to 100% by mass in terms of dry mass, preferably 1 to 85% by mass. It is more preferable that it is contained in%, and it is further preferable that it is contained in an amount of 5 to 70% by mass.

In order to exert the effect of the present invention more effectively, it is preferable that the component of the present invention is contained in an amount of 80% or more, and 90% or more, of the entire oral composition of the present invention in terms of dry mass. Is more preferable, 95% or more is further preferable, and 100% is particularly preferable.

The compounding mass ratio of the pine bark extract and the plant material in the oral composition of the present invention is preferably in the range of 1: 0.001 to 1: 1000 in terms of dry mass, and is 1: 0.01. It is more preferably in the range of ~ 1: 500, and even more preferably in the range of 1: 0.1 to 1: 200.

The intake of the oral composition of the present invention is not particularly limited, but from the viewpoint of exerting the effect of the present invention more remarkably, the daily intake of pine bark extract for adults is 0.5 mg / day or more. It is preferable to take it so as to be 1 mg / day or more, and it is more preferable to take it so as to be 1.5 mg / day or more. The upper limit is, for example, 500 mg / day, preferably 400 mg / day. The oral composition of the present invention is not particularly limited as long as it requires oral care, but is said to be about 80% of adults in their 30s and 40s who have periodontal disease, and elderly people with weak gums. Is preferable to ingest.

The oral composition of the present invention can be contained in one container or divided into a plurality of containers, for example, 2 to 3 so that the daily intake becomes the above-mentioned intake.

The oral composition of the present invention can be produced by a known method by adding components other than the components of the present invention that are acceptable for oral use, if necessary. Examples of components other than the components of the present invention include water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, and vitamin P. ), Vitamins such as oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus, iron; sulfur-containing compounds contained in taurine, garlic, etc .; flavonoids such as hesperidin, quercetin, etc. Flavonoids; proteins such as collagen; peptides; amino acids; animal fats and oils; vegetable fats and oils; crushed products or extracts of animals and plants.

Hereinafter, the present invention will be described based on examples.

[Example 1]
(Test method)
Porphyromonas gingivalis (P. gingivalis) was anaerobic using Aneropack Kenki (manufactured by Mitsubishi Chemical Corporation) and a square jar (manufactured by Mitsubishi Chemical Corporation), and a modified GAM bouillon (Nissui) was sterilized by high-pressure steam. It was cultured at 37 ° C. (manufactured by Pharmaceutical Corporation).
P. gingivalis was diluted with Dulbeccoline phosphate buffered saline (manufactured by Nacalai Tesque, Inc.) to prepare a turbidity of 0.5 McFarlamd. McFarland Standard (manufactured by Sysmex BioMérieux Co., Ltd.) was used as an index of turbidity.

A modified GAM agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was sterilized by high-pressure steam and then poured into a sterilized petri dish to prepare a plate medium. P. gingivalis prepared to the above 0.5 McFarland was uniformly applied to the plate medium.

The test substance (pine bark extract and / or auxiliary material) was prepared to a predetermined concentration using PBS or DMSO. 15 μL of the test substance was added dropwise to a 6 mm filter paper (manufactured by GE Healthcare) for antibiotic assay, and the substance was attached to a petri dish coated with P. gingivalis.

The cells were cultured in an anaerobic environment at 37 ° C. using Aneropack Kenki, a square jar and a homeothermic incubator for 2 to 3 days.

The diameter of the blocking circle of each test substance formed in the petri dish was measured, and the diameter of the antibiotic filter paper (6 mm) was divided. The growth inhibitory effect of each test substance was corrected by dividing the diameter of the isodine blocking circle provided as a positive control on each plate by 6 mm (diameter of the test substance blocking circle -6 mm (mm) / diameter of the isodine blocking circle- 6 mm (mm)). The growth inhibitory effect of each test substance was calculated as a ratio to the pine bark extract alone (50 mg / mL), and the growth inhibitory effect of each test substance on P. gingivalis was evaluated.

As the test substance, the following commercially available products or preparations were used.
[Pine bark extract]
A pine bark extract (Toyo Shinyaku Co., Ltd .; Flavangenol (registered trademark)), which is a French coastal pine bark extract, was used.

[Secondary material]
For coffee robusta, commercially available coffee seed (raw coffee beans) extract powder was used.
For the linden tree (Tilia cordata Mill.), Commercially available flower extract powder was used.
For Kudzu flower (Pueraria thomsonii), the extract powder of the flower extracted with hot water (Toyo Shinyaku Co., Ltd.) was used.
For the young barley leaves, the extract powder obtained by extracting the crushed powder (Toyo Shinyaku Co., Ltd.) with hydrous ethanol was used.
For the young sweet potato leaves, the extract powder obtained by extracting the crushed powder (Toyo Shinyaku Co., Ltd.) with hydrous ethanol was used.

The evaluation results of the growth inhibitory effect of each test substance on P. gingivalis are shown in FIGS. 1 to 5. FVG in the figure means a pine bark extract (flavangenol (registered trademark)).

As shown in FIGS. 1 to 5, it can be seen that the combination of the pine bark extract and a specific plant material effectively suppresses the growth of P. gingivalis. In particular, although coffee tree, linden tree, barley young leaves and sweet potato young leaves alone have weak or no effect, the growth inhibitory effect on P. gingivalis was effectively enhanced by combining with the pine bark extract. Kuzuhana itself had a growth inhibitory effect, but the effect was further enhanced by combining it with the pine bark extract. Therefore, the oral composition of the present invention prevents and / or improves periodontal disease by effectively suppressing the growth of P. gingivalis, and also prevents and / or improves halitosis peculiar to periodontal disease. It turns out that it is an excellent oral composition for oral care.

[Example 2] (Production of tablets)
Pine bark extract 12 mg
Bodhi tree extract powder (derived from flowers) 200 mg
Sucrose fatty acid ester 350 mg
Shellac 10 mg
Fragrance 2mg
The mixture consisting of the reduced maltose residue was tableted to produce four 300 mg tablets.

[Example 3] (Manufacturing of chewable tablet)
Pine bark extract 1.5 mg
Coffee tree extract powder (derived from raw coffee beans) 150 mg
Hydroxypropyl cellulose 250 mg
Sucrose fatty acid ester 50 mg
Aspartame 30 mg
Fragrance 3mg
The mixture consisting of the reduced maltose residue was tableted to produce two 500 mg chewable tablets.

[Example 4] (Production of granules)
Pine bark extract 50 mg
Barley young leaf powder 2000 mg
Fructooligosaccharide 200mg
A mixture consisting of the residue of reduced maltose was mixed and then granulated to produce 3 g of granules.

[Example 5] (Production of powder)
Pine bark extract 100 mg
Sweet potato young leaf powder 1500 mg
Tea extract 50mg
The mixture consisting of the dextrin residue was mixed to produce 3 g of powder.

[Example 6] (Manufacturing of candy)
Pine bark extract 30 mg
Coffee tree extract powder (derived from raw coffee beans) 50 mg
Bodhi tree extract powder (derived from flowers) 20 mg
Sweetener (aspartame / L-phenylalanine compound,
Acesulfame K, Stevia) 30 mg
Fragrance 10 mg
One 3 g candy consisting of the residue of reduced maltose starch syrup was produced according to a conventional method.

[Example 7] (Manufacturing of packaged beverage)
Pine bark extract 200 mg
Kuzuhana extract 35mg
Black tea leaf extract powder 500mg
Sodium ascorbate 50 mg
A 350 ml packaged beverage consisting of a water residue was produced according to a conventional method.

Since the oral composition of the present invention can be used as a health food or the like having a high periodontal disease prevention and / or ameliorating effect, the industrial usefulness of the present invention is high.

Claims (3)

An oral composition for oral care comprising a pine bark extract and at least one plant material selected from young bark leaves, kudzu flowers, young sweet potato leaves and linden trees. An oral composition for oral care containing a pine bark extract as an active ingredient, further containing at least one plant material selected from young barley leaves, kudzu flowers, young sweet potato leaves and linden trees. Composition. An oral composition comprising a pine bark extract and a linden tree.

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