JP2022084222A - Isolation method applied for silver nano curcuma material for suppressing new coronavirus - Google Patents

Abstract

To provide an isolation method applied for silver nano curcuma material for suppressing new coronavirus, the silver nano curcuma material capable of being applied for suppression of the new coronavirus and a similar virus, and in the future, capable of serving as a medicine source for preventing and treating a disease derived from the new coronavirus COVID-19.SOLUTION: An isolation method applied for silver nano curcuma material for suppressing new coronavirus is configured to heat, agitate curcuma powder, then mix a silver nitrate solution with the curcuma powder, lower a temperature of the mixture and then agitate the mixture, and after reaction and centrifugal separation and the like, extract a silver nano curcuma crude extract. Then, after washing and centrifugal separation and the like, a purified silver nano curcuma composition is acquired.SELECTED DRAWING: Figure 1

Description

The present invention relates to a preparative method applied to a silver nano-Ukon material that suppresses a new coronavirus, a silver nano-Ukon material that is applied to virus suppression and prevents and treats a disease derived from the new coronavirus COVID-19 in the future. Involved in the technical area of manufacturing.

According to Google Internet data, it is the highly contagious new coronavirus (COVID-19) that has recently caused people anxiety, and as of October 11, 2020, it is already 37.3 million worldwide. The number of people who have been confirmed positive and the number of deaths from infection has reached 1.07 million, and the number is still rising at a tremendous speed.

Coronavirus (CoV) is an RNA virus with an envelope and is named because it resembles a crown protrusion when viewed under an electron microscope. Coronavirus has been the cause of severe acute respiratory disease, 2003 severe acute respiratory syndrome coronavirus (SARS-CoV), 2013 Middle East respiratory syndrome coronavirus (MERS-CoV), As of 2019, SARS-CoV-2, which has caused serious harm and is prevalent all over the world, has been named by the World Health Organization as the new coronavirus COVID-19. RNA viruses have a very high mutation rate, so RNA viruses quickly develop drug resistance and invalidate vaccines. AIDS (AIDS) is an RNA virus, a well-known human disease, and to date no vaccine has been published to treat AIDS. The cause is the high mutational properties of RNA virus.

The inventor has been studying the application of silver nano and turmeric for a long time, and discovered that the binding between silver nano and turmeric has an inhibitory effect on viruses.

Silver nano has antibacterial activity, antifungal and antiviral properties and silver compounds have been extensively studied. Silver nano is used in textile products, wound dressings and medical machinery. Silver nanoantibacterial agents are equally effective against Gram-positive and Gram-negative bacteria, and the literature states that silver nanos have been proven to have antibacterial effects against bacteria in in vitro experiments. For example, methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli, Pseudomonas aeruginosa, Vibrio cholerae and Bacillus subtilis all have good bacteriostatic effects. .. Silver nano also has a good bacteriostatic effect on fungi such as black Aspergillus niger and Candida albicans. Similarly, silver nano also has an effective antiviral effect against various viruses. As an example, AIDS virus (Human i mmolunodeficiency virus, HIV), respiratory vesicle virus (Respiratory synchronous virus, RSV), Takaribe virus (Tacaribe virus, TCRV), type 1 simple herpes virus (TCRV). 1) There are monkey pox virus (Monkeypox virus) and hepatitis B virus (Hepatitis B virus, HBV). Therefore, silver nano has a wide variety of mechanisms against the suppression and killing of bacteria and viruses, can destroy the envelope of viruses, and can destroy the activities of viruses such as DNA, RNA, and proteins, and viruses. Kill. Even if the virus is mutated, it does not affect the killing properties of the silver nanovirus. In order for silver nanoparticles to interact effectively with the virus, the silver nanoparticles must be smaller than the virus. As an example, it is better if it is smaller than 10 nm or smaller than 10 nm, and most preferably in the range of 3 nm-7 nm. When the virus is fully covered with silver nanoparticles, viral function is interfered with.

Among turmeric (scientific name is Curcuma longa L, English name is Turmeric), the active ingredient curcumin (molecular formula C21H20O6) is a compound composed of elements such as carbon (C), hydrogen (H) and oxygen (O). be. Curcumin has already been approved by the US Food and Drug Administration (FDA), and in more than 300 clinical trials, curcumin has inflammatory diseases, nervous system diseases, cardiovascular diseases, lung diseases, metabolic diseases, liver diseases and cancer. It has been reported to be beneficial for the protective action of various diseases including diseases. In addition, a large amount of research literature states that turmeric and curcumin can counter various viruses. Examples include AIDS virus (Human immolunodefiency virus, HIV), influenza virus (Influenza virus), type 1 and type 2 simple herpes virus (Herpes simplex virus, HSV-1 and HSV-2), coxsackie virus (Coxyvirus). Virus (Hepatitis B Virus, HBV), Hepatitis C virus (Hepatitis C virus, HCV), Human Papilloma virus (HPV), Japanese encephalitis virus (Japanese Encephaltis) Human virus There is a virus type 1 (Human T-lymactive virus 1, HTLV-1), and it has been proved very much that ukon and curcumin have an action of suppressing the infection of various viruses.

The present invention can be applied to the suppression of new coronavirus and similar viruses by fractionating using silver nano and turmeric, and further, as a drug source for preventing and treating diseases derived from the new coronavirus COVID-19 in the future. Become.

The main purpose of the preparative method applied to the silver nano-Ukon material that suppresses the new coronavirus provided by the present invention is to improve the global human health and economic damage caused by the new coronavirus COVID-19, and is currently present. Human beings break through the stagnation period of prevention and treatment of diseases derived from the new coronavirus. Mainly turmeric, silver nano is supplemented as a biological nanomaterial. Curcumin and silver nano can simultaneously obtain the effect of suppressing viruses, and also solve problems such as curcumin being difficult to dissolve in water and therefore difficult to be absorbed by the human body. At the same time, it can avoid the biological irritation problem caused by the single use of silver nano, and in the future, it will be a drug source for preventing and treating diseases derived from the new coronavirus, and effectively and safely suppress the new coronavirus COVID-19. Provide as a drug to do. It may also compensate for the high mutation rates of the current new coronavirus, and future vaccines or other related treatments cannot completely treat the solution to the new coronavirus epidemic, thereby causing the new corona. Improve the global health and economic problems of the illness.

In order to achieve the above object, the specific sorting method of the present invention includes the following steps.
In step 1, turmeric powder is mixed with deionized water.
Step 2 heats and brings to a boil.
In step 3, the temperature is lowered and then stirring is performed.
Step 4 mix the silver nitrate solution.
In step 5, the temperature is lowered, and then the mixture is stirred and reacted.
Step 6 centrifuges.
In step 7, the supernatant liquid is taken out.
In step 8, the supernatant liquid is separated by centrifugation.
Step 9 collects the precipitate to obtain a crude extract of silver nano-Ukon.
In step 10, deionized water is added and vibration mixed cleaning is performed at high speed.
Step 11 performs centrifugation.
Step 12 collects the precipitate.
Step 13 performs multiple washes and centrifugations.
Step 14 obtains a purified silver nano-Ukon composition.

As a good example, in step 1, 5 grams of turmeric powder is weighed and 500 milliliters of deionized water is poured.

As a good example, in step 2, deionized water mixed with turmeric powder in step 1 is heated and boiled. The boiling time is 5 minutes.

As a good example, in step 3, the liquid in step 2 is cooled down and then slowly agitated. The temperature is 75 to 85 ° C, with 85 ° C being the best.

As a good example, in step 4, 5 ml 1 mmol of silver nitrate solution is slowly added at the same time as stirring in step 3.

As a good example, in step 5, the temperature of the liquid in step 4 is lowered to 45-55 ° C. 50 ° C. is best, and after stirring slowly for 24 hours, the reaction is stopped.

As a good example, in step 6, centrifugation is performed for 10 minutes at 4 ° C. and 3000 rpm using a high speed centrifuge to remove precipitated impurities.

As a good example, in step 7, the supernatant liquid from which the precipitated impurities have been removed is taken out from the liquid after centrifugation in step 6.
In step 8, the supernatant of step 7 is centrifuged at 4 ° C. and 14000 rpm for 1 hour using a high-speed centrifuge.

As a good example, in step 9, the precipitate is collected and the precipitate is a crude extract of silver nano-Ukon.
In step 10, 50 ml of deionized water is added and the crude silver nano-Ukon extract is vibrated and mixed and washed at high speed.

As a good example, in step 11, the crude silver nano-Ukon extract is centrifuged at 4 ° C. and 14000 rpm for 1 hour using a high speed centrifuge.
In step 12, the precipitate from step 11 is collected.
In step 13, the precipitate of step 12 is washed and centrifuged at 4 ° C. and 14000 rpm a plurality of times using a high-speed centrifuge to remove residual silver nitrate.

It is a block diagram of the flowchart of the step of this invention.

(One embodiment)
The following is an attached diagram of an embodiment of the present invention, which clearly describes the technical method of the embodiment of the present invention. The following is a detailed description of the present invention so that it can be fully understood. However, the present invention may be implemented in other different ways, and engineers proficient in this area may present similar developments in the context of the present invention. Therefore, the present invention is not limited to the following specific examples.

FIG. 1 shows a method for sorting the silver nano-Ukon material of the present invention, which comprises the steps described below and is carried out in the following order.

In step 1, turmeric powder is mixed with deionized water.
Step 2 heats and brings to a boil.
In step 3, the temperature is lowered and then stirring is performed.
Step 4 mix the silver nitrate solution.
In step 5, the temperature is lowered, and then the mixture is stirred and reacted.
Step 6 centrifuges.
In step 7, the supernatant liquid is taken out.
In step 8, the supernatant liquid is separated by centrifugation.
Step 9 collects the precipitate to obtain a crude extract of silver nano-Ukon.
In step 10, deionized water is added and vibration mixed cleaning is performed at high speed.
Step 11 performs centrifugation.
Step 12 collects the precipitate.
Step 13 performs multiple washes and centrifugations.
Step 14 obtains a purified silver nano-Ukon composition.

Of these, in step 1, 5 grams of turmeric powder is weighed and 500 milliliters of deionized water is poured.
In step 2, the deionized water mixed with turmeric powder in step 1 is heated and boiled. The boiling time should be 5 minutes.
In step 3, the temperature of the liquid in step 2 is lowered, and then the mixture is slowly stirred. The temperature is between 75 and 85 ° C, with 85 ° C being the best.
In step 4, 5 ml of 1 mmol of silver nitrate solution is slowly added at the same time as stirring.
In step 5, the temperature of the liquid in step 4 is lowered to 45 to 55 ° C. 50 ° C. is best, and after stirring slowly for 24 hours, the reaction is stopped.
In step 6, a high speed centrifuge is used at 4 ° C. and 3000 rpm for 10 minutes to remove the precipitated impurities.
In step 7, the supernatant liquid from which impurities precipitated are removed from the liquid after centrifugation in step 6 is taken out.
In step 8, the supernatant of step 7 is centrifuged at 4 ° C. and 14000 rpm for about 1 hour using a high-speed centrifuge.
In step 9, the precipitate is collected. The precipitate is a crude extract of silver nano-Ukon.
In step 10, 50 ml of deionized water is added and the crude silver nano-Ukon extract is vibrated and mixed and washed at high speed.
In step 11, the crude silver nano-Ukon extract is centrifuged at 4 ° C. and 14000 rpm for 1 hour using a high-speed centrifuge.
In step 12, the precipitate from step 11 is collected.
In step 13, the precipitate of step 12 is centrifuged at 4 ° C. and 14000 rpm for 1 hour using a high-speed centrifuge. Perform multiple washes and centrifugations, for example 10 times, to remove residual silver nitrate.
Finally, in step 14, a purified silver nanocurcumin composition is obtained (Silver-nanocurcumin).

The silver nano-Ukon composition (Silver-nanocurcumin) can also be mixed with pure water to make a nasal spray at the time of application. Alternatively, it can be mixed with various solids or liquids to form a powdery, liquid or gel antiviral antibacterial product.

As a supplementary explanation, silver nano does not pose a safety problem to the human body in normal use. Because the human body ingests silver elements from food and water every day, the human body has a normal mechanism of metabolism and excretion of silver. Most foods contain silver in the range of 10-100 μg / kg. In addition, the silver element is excreted within a few days to 50 days during the half-life of the human liver, and most of the absorbed silver is excreted together with the bile in the feces. Therefore, silver nano is not permanently accumulated in human tissues.

After research, the inventor discovered that the silver-nanocurcumin of the present invention can prevent infection with the new coronavirus COVID-19 in a healthy body.
By injecting silver nano-Ukon into the nasal cavity as a nasal spray, the virus in the nasal cavity can be killed within a certain period of time. By spraying locally into the nasal cavity multiple times a day, silver nano-Ukon can stay in the nasal cavity to kill the virus and block cell-mediated immunity infections.

The silver nanourcumin composition (Silver-nanocurcumin) fractionated by the present invention can be applied to a drug source that suppresses a new coronavirus and other similar viruses, and further prevents and treats a disease derived from the new coronavirus COVID-19. Besides, it can be an odorless, harmless, non-toxic and non-irritating organism anti-virus material, cleaning tables, masks, door knobs, mobile phones, elevator buttons and other normal hygiene products, It can be applied to disinfection, sterilization, and antibacterial action. Furthermore, it can be applied to medical disinfection and wound healing, research and development application of subsequent drugs such as influenza virus and enterovirus, food processing and disinfection, disinfection of fruits and vegetables, disinfection of frozen foods, and hygiene work of cosmetics.
In addition, by performing air disinfection in a closed space (for example, any central air conditioning equipment for airplanes, vehicles, ships, and skyscrapers), the aerosol content of COVID-19 in the air can be reduced, and COVID-19 can be used. Reduces transmission speed and capacity.

It is clear from the above that the indicated examples are only a part of the examples of the present invention, not all of the examples. Among the examples based on the present invention, all other examples that can be conceived by a general engineer who is proficient in this area without exercising creativity shall belong to the claims of the present invention.
The particle size of the silver nano-Ukon is not limited, but may be, for example, 3 nm to 10 nm.

S1 Mix turmeric powder with deionized water S2 Heat and boil S3 Lower the temperature and then stir S4 Mix the silver nitrate solution S5 Lower the temperature and then stir and react S6 Centrifuge S7 Take out the supernatant S8 Centrifuge the supernatant S9 Collect the precipitate to obtain a crude extract of silver nano-Ukon S10 Add deionized water and vibrate-mix and wash at high speed S11 Centrifuge S12 Collect the precipitate S13 Multiple washings And centrifuge to obtain a S14 purified silver nano-Ukon composition.

Claims (10)

In the preparative method applied to the silver nano turmeric material that suppresses the new coronavirus, among them, the following steps are included.
In step 1, turmeric powder is mixed with deionized water, and the mixture is mixed with turmeric powder.
Step 2 is to heat and bring to a boil.
In step 3, the temperature is lowered, then the mixture is stirred, and the temperature is lowered.
Step 4 mix the silver nitrate solution and
In step 5, the temperature is lowered, and then the mixture is stirred and reacted.
Step 6 centrifuges and
In step 7, the supernatant liquid is taken out and
Step 8 centrifuges the supernatant and
Step 9 collects the precipitate to obtain a crude extract of silver nano-Ukon.
In step 10, deionized water is added, and vibration mixing and washing is performed at high speed.
In step 11, centrifuge and
Step 12 collects the precipitate and
Step 13 involves performing multiple washes and centrifugation.
Step 14 is a preparative method applied to a silver nano-turmeric material that suppresses a new type of coronavirus, which comprises obtaining a purified silver nano-turmeric composition. The preparative method according to claim 1, wherein step 1 is applied to a silver nano-turmeric material that suppresses the new coronavirus according to claim 1, wherein 5 grams of turmeric powder is weighed and 500 ml of deionized water is poured. .. In step 2, the deionized water mixed with turmeric powder in step 1 is heated and boiled, and the boiling time is 5 minutes. The silver nano-turmeric that suppresses the new coronavirus according to claim 2. A preparative method applied to materials. The silver nanoturmeric material for suppressing the new coronavirus according to claim 3, wherein in step 3, the temperature of the liquid in step 2 is lowered and then stirred, and the temperature is between 75 and 85 ° C. A preparative method applied to. The preparative method applied to the silver nano-turmeric material that suppresses the new coronavirus according to claim 4, wherein in step 4, 5 ml of 1 mmol of silver nitrate solution is added at the same time as stirring in step 3. The silver nanoturmeric material for suppressing the new coronavirus according to claim 5, wherein in step 5, the temperature of the liquid in step 4 is lowered to 45 to 55 ° C., the mixture is stirred for 24 hours, and then the reaction is stopped. A preparative method applied to. In step 6, the silver nanoturmeric material for suppressing the new coronavirus according to claim 6, which is characterized by centrifuging at 4 ° C. and 3000 rpm for 10 minutes using a centrifuge to remove precipitated impurities. Applied sorting method. In step 7, the supernatant liquid from which impurities precipitated have been removed from the liquid after centrifugation in step 6 is taken out.
The silver nano turmeric material for suppressing the new coronavirus according to claim 7, wherein in step 8, the supernatant of step 7 is centrifuged at 4 ° C. and 14000 rpm for 1 hour using a centrifuge. Separation method applied to. In step 9, the precipitate is collected and the precipitate is a crude extract of silver nano-Ukon.
The preparative method applied to the silver nano-turmeric material that suppresses the new coronavirus according to claim 8, wherein in step 10, 50 ml of deionized water is added to vibrate-mix and wash the crude silver nano-turmeric extract. .. In step 11, the crude silver nano-Ukon extract was centrifuged at 4 ° C. and 14000 rpm for 1 hour using a centrifuge.
In step 12, the precipitate from step 11 is collected and
The new corona according to claim 9, wherein in step 13, the precipitate of step 12 is washed and centrifuged a plurality of times using a centrifuge at 4 ° C. and 14000 rpm to remove residual silver nitrate. A precipitation method applied to silver nano turmeric materials that suppress viruses.

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