JP2022074510A - Composition for oral care - Google Patents

Abstract

To provide a composition for oral care that continuously inhibits the growth of bacteria on the surface of a tongue and exerts a superior tongue coating removal effect.SOLUTION: A composition for oral care contains two or more selected from the group consisting of rosemary extract, propolis extract, Pterocarpus marsupium extract, and Carica papaya extract as active ingredients.SELECTED DRAWING: None

Description

The present invention relates to a composition for oral care.

In recent years, the number of people who have oral problems such as tooth decay, periodontal disease and bad breath is increasing, and the demand for oral care products that can be used on a daily basis is increasing. Oral care products include oral care products such as mouthwashes, oral care tools such as tongue brushes, quasi-drugs such as periodontal disease treatment / preventive agents, and oral care foods such as oral care tablets. Yes (Non-Patent Document 1). Among them, there are a wide variety of oral care products used for the purpose of removing tongue coating, which is the main cause of bad breath. Conventionally, tongue brushes have been mainly used, but in recent years, oral care tablets, which are oral care foods, have been attracting attention.

Tongue coating is an aggregate of microorganisms, food residues, shed epithelium, etc. attached to a portion called the dorsal consonant on the surface of the tongue. As mentioned above, it is widely known that tongue coating is the cause of bad breath, but it is also speculated that it plays a role as a source of bacteria to each oral cavity, and the effect of tongue coating bacteria on the entire oral cavity is small. It is said that there is no such thing (Non-Patent Document 2). It is thought that a wide variety of bacteria are present in the tongue moss, but the main bacteria are the genus Candida such as Candida glabrata and the genus Streptococcus such as Streptococcus mutans. Bacteria are known (Non-Patent Document 3).

Removal of tongue coating is also important in preventive medicine. Oral care, including removal of tongue moss, has been shown to reduce the total number of microorganisms sucked into the lower respiratory tract, and oral care, including removal of tongue moss, is primarily brought about by anaerobic bacteria inhabiting the oral cavity and pharynx. It has been clarified that it plays an important role in the prevention of aspiration pneumonia and the like (Non-Patent Document 4).

By the way, although the removal of tongue coating by an instrument such as a tongue brush has a certain effect as described above, it is only a temporary removal and cannot suppress the growth of bacteria and the regeneration of tongue coating in the tongue coating. In fact, a dental hygienist provided oral hygiene guidance, including cleaning of the back of the tongue with a brush and other equipment, to the elderly living in a special elderly nursing home. The number of bacteria did not decrease (Non-Patent Document 5).

Based on the above facts, the evaluation method for tongue coating removal effect currently relies mainly on subjective information such as visual evaluation of tongue coating on the back of the tongue coating, but in order to objectively evaluate it, the number of bacteria is used. It can be said that it is important to carry out a combination of quantitative evaluations such as fluctuations (this is also proposed in Non-Patent Document 6).

On the other hand, as a prior art for removing tongue coating, there is a food for removing tongue coating containing a purified actinidin and an excipient (Patent Document 1). Here, the amount of tongue coating before and after the use of the protease (actinidin) -containing tablet and the fake agent is compared, and it is disclosed that the amount of tongue coating adhered was significantly reduced in the group using the protease-containing tablet. Further, there is a prior art regarding a method for producing a tablet for removing tongue coating, which is a mixture of a highly soluble saccharide and a low solubility saccharide (Patent Document 2). Here, the physical effect of removing tongue coating by the undulations of the tablet surface is disclosed. However, none of these prior arts have touched on the suppression of bacterial growth in tongue coating.

Prior arts for controlling oral bacteria include periodontal disease-preventing oral compositions using shakuyaku extract, otogirisou extract, hop extract, birch extract, ginger extract, or rosemary extract (patented). Document 3). Here, we are investigating an oral composition using a material that suppresses bacteria that cause periodontal disease, but we have not touched on the suppression of bacterial growth in tongue coating. We also found that an extract of Indian mushroom has an inhibitory effect on the release of matrix metalloproteinase-1 (MMP-1) from gingival fibroblasts, and provided this as an ameliorating periodontal disease that causes halitosis. There is also a prior art (Patent Document 4). However, this also does not focus on the suppression of bacterial growth in tongue coating. That is, no oral care material has been known so far that can be expected to continuously suppress the growth of bacteria in tongue coating and suppress the reformation of tongue coating.

Although many studies have been conducted on the removal of tongue coating and the suppression of periodontal disease bacteria, there is no report that the removal of tongue coating and the reduction of the number of bacteria on the back of the tongue can be achieved at the same time.

As described above, the conventional technique for removing tongue coating has a problem that bacteria on the surface of the tongue grow over time and the tongue coating is easily reformed and the effect of removing tongue coating does not last long.

International Publication No. 2003/090704 International Publication No. 2007/026755 Japanese Unexamined Patent Publication No. 2019-196328 Japanese Patent No. 6551924

Oral Care Related Market Marketing Overview (2019) Journal of the Japan Dental Association, 64,717-727 (2011) Microbial Pathogenesis, 127, 97-105 (2019) Dental Bulletin, 112, 620-623 (2012) Geriatric Dentistry, 14,297-306 (2000) Journal of the Japanese Society of Health Sciences, 28, 2-9 (2019)

Therefore, in view of the above circumstances, an object of the present invention is to provide a composition for oral care which continuously suppresses the growth of bacteria in tongue coating and has an excellent effect of removing tongue coating.

As a result of diligent studies for the above purpose, the present inventors have found that the rosemary extract, the propolis extract, the Indian mushroom extract, and the papaya extract are surprisingly the main fungi of the tongue moss, Candida spp. It was found that the growth was effectively suppressed, and that the rosemary extract, the propolis extract, and the Indian mushroom extract also effectively suppressed the growth of Streptococcus spp. Furthermore, the present inventors have found that by blending two or more of these materials in combination, the growth of bacteria in tongue coating is continuously suppressed and an excellent tongue coating removing effect can be obtained. Completed. That is, the gist of the present invention is as shown below.

[1] An oral care composition containing two or more selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient.
[2] The composition for oral care according to [1], which comprises at least one selected from the group consisting of rosemary extract, propolis extract, and Indian mushroom extract; and papaya extract as an active ingredient.
[3] The composition for oral care according to [1] or [2], which contains a rosemary extract, a propolis extract, and a papaya extract as active ingredients.
[4] The oral care product according to any one of [1] to [3], wherein the total content of the rosemary extract, the propolis extract, and the Indian crab extract is 10 to 90% by weight in the total amount of the composition. Composition.
[5] The weight ratio of the total content of the rosemary extract, the propolis extract, and the Indian mushroom extract to the content of the papaya extract is 1: 9 to 9: 1, [2] to [4]. The composition for oral care according to any one of.
[6] An oral care composition for removing tongue coating, which comprises at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient.
[7] The composition for oral care according to any one of [1] to [6], which is a tablet.
[8] A composition for oral care for suppressing the growth of Candida glabrata, which contains at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient.
[9] A composition for oral care for suppressing the growth of Streptococcus mutans, which contains at least one selected from the group consisting of rosemary extract, propolis extract, and Indian mushroom extract as an active ingredient.

According to the present invention, it is possible to provide a composition for oral care that continuously suppresses the growth of bacteria in tongue coating and has an excellent tongue coating removing effect.

FIG. 1 is a graph showing the results of antibacterial test 1. FIG. 2 is a graph showing the results of the antibacterial test 2. FIG. 3 is a graph showing the results of a bacterial suppression test in tongue coating when various active ingredients are ingested alone. FIG. 4 is a graph showing the results of a bacterial suppression effect test when ingesting Tablet 1 (invention product), Tablets 2 and 3 (predecessor product). FIG. 5 is a photograph showing the state of tongue coating immediately before, immediately after, and 4 hours after ingestion of tablet 1 (invention product). FIG. 6 is a graph showing the results of the continuous test: the effect of suppressing bacteria in tongue coating when ingesting Tablet 1 (invention product). FIG. 7 is a photograph showing the effects of ingesting tablet 1 (invention product), ingesting tablet 3 (predecessor product), and removing tongue coating by cleaning the tongue brush.

Hereinafter, the present invention will be described in detail. The terms used herein are to be construed as commonly used in the art, unless otherwise noted.

[Composition for oral care]
The composition for oral care of the present invention contains, as an active ingredient, two or more selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract. The composition for oral care of the present invention has, as active ingredients, a rosemary extract, a propolis extract, and an Indian mushroom extract having an action of suppressing the growth of Candida spp., Which is a major fungus of tongue coating, and Streptococcus spp. By blending two or more of the papaya extracts having the effect of suppressing the growth of Candida spp. In addition to the substance, the growth of the fungus in tongue coating is continuously suppressed and an excellent tongue coating removing effect is exhibited. do.

<Active ingredient>
The active ingredients in the present invention are rosemary extract, propolis extract, Indian mushroom extract, and papaya extract. The oral care composition of the present invention may contain two or more of these active ingredients, and the combination thereof is arbitrary.

Specific combinations of the two active ingredients include rosemary extract and propolis extract, rosemary extract and indian mushroom extract, rosemary extract and papaya extract, propolis extract and indokinoki extract, and propolis extract. Examples include combinations of propolis extract and propolis extract, and indokinoki extract and propolis extract.

Among the materials related to the active ingredient in the present invention, the rosemary extract, the propolis extract, and the tongue coating extract are water-insoluble, and are, for example, water-soluble materials (for example, excipients) when molded into a tablet or the like. When combined with (such as), the surface of the tablet becomes sardine in the oral cavity (particularly on the tongue), so the effect of removing tongue coating can be further enhanced. Therefore, in the oral care composition of the present invention, it is preferable that one or more of the water-insoluble rosemary extract, propolis extract, and Indian mushroom extract are selected and blended. Further, among the active ingredients in the present invention, since the papaya extract is water-soluble, for example, when molded into a tablet or the like, when combined with the water-insoluble active ingredient in the present invention, the water in the present invention is used. A higher tongue moss removing effect can be obtained than when the insoluble active ingredient is combined with a simple water-soluble material (for example, an excipient). Therefore, in the oral care composition of the present invention, it is more preferable that one or more of the rosemary extract, the propolis extract, and the Indian mushroom extract, and the papaya extract are selected and blended.

In the present invention, the total content of the rosemary extract, the propolis extract, and the Indian mushroom extract in the total amount of the composition can be, for example, 10 to 90% by weight, preferably 20 to 70% by weight. Is. By adjusting the water-insoluble active ingredient in the composition to such a range, it is possible to efficiently add the effect of the active ingredient itself to the effect of suppressing the bacteria by making the shape of the composition file-like. The effect of removing tongue coating can be further enhanced. The content of the papaya extract in the water-soluble component of the composition can be, for example, 10 to 90% by weight, preferably 30 to 80% by weight. By adjusting the active ingredient in the water-soluble component of the composition within such a range, it is possible to more efficiently add the effect of suppressing the bacteria of the active ingredient itself and the effect of forming the shape of the composition into a file shape. It can further enhance the effect of removing tongue coating. From this point of view, the weight ratio of the total content of rosemary extract, propolis extract, and Indian mushroom extract to the content of papaya extract (total of rosemary extract, propolis extract, and Indian mushroom extract). Content: content of papaya extract) is preferably 1: 9 to 9: 1, more preferably 1: 2 to 2: 1.

(Rosemary extract)
In the present invention, the rosemary extract uses a water-insoluble extract. For example, the leaves, stems or whole plants of Labiatae, Rosmarinus, and Rosemary (Rosmarinus officinalis L.) can be used as they are or dried to make an extract material (the material may be appropriately pulverized). ), Polar solvent (types of solvent include, for example, methanol, ethanol, propanol, isopropanol, butanol, isobutanol, etc., preferably ethanol) and water-insoluble extracted by a general method. Extracts can be used. A commercially available rosemary extract can also be used in the present invention, and any one or a plurality of commercially available rosemary extracts can be used in combination.

In the present invention, the rosemary extract can be contained, for example, in an amount of 0.1 to 90% by weight, preferably 0.5 to 50% by weight, based on the total amount of the oral care composition. ..

(Propolis extract)
Propolis is a glue-like substance formed by mixing sap collected mainly from tree buds and bark of bees with beeswax and enzymes in the saliva of bees.

In the present invention, the propolis extract uses a water-insoluble extract. For example, propolis can be used as it is or dried to obtain an extraction material (the material may be appropriately pulverized), and a polar solvent (the type of solvent may be, for example, methanol, ethanol, propanol, isopropanol, butanol, isobutanol, etc.). , And preferably ethanol), and water-insoluble extracts extracted by a general method can be used. A commercially available propolis extract can also be used in the present invention, and any one or a plurality of commercially available propolis extracts can be used in combination.

In the present invention, the propolis extract can be contained, for example, in an amount of 0.1 to 90% by weight, preferably 0.5 to 50% by weight, based on the total amount of the oral care composition.

(Indian mushroom extract)
Red sandalwood is another name for the leguminous family (Fabaceae), the genus Pterocarpus, and the Pterocarpus marsupium.

In the present invention, the Indian mushroom extract uses a water-insoluble extract. For example, the trunk or whole tree of Indian mushroom can be used as an extract material as it is or dried (the material may be appropriately pulverized) and a polar solvent (the type of solvent is, for example, methanol, ethanol, propanol, isopropanol, etc.). A water-insoluble extract extracted by a general method using butanol, isobutanol and the like, preferably ethanol can be used. Further, a commercially available Indian mushroom extract can also be used in the present invention, and any one or a plurality of commercially available Indian mushroom extracts can be used in combination.

In the present invention, the Indian mushroom extract can be contained, for example, in an amount of 0.1 to 90% by weight, preferably 0.5 to 50% by weight, based on the total amount of the oral care composition.

(Papaya extract)
In the present invention, the papaya extract uses a water-soluble extract. For example, a squeezed solution obtained by squeezing the fruits of Caricaceae, Carica, and Papaya L can be used as a papaya extract (extract). Further, from a material obtained by crushing papaya nuts, a polar solvent (examples of the solvent include water, physiological saline, various aqueous buffers, and preferably water) is used. A water-soluble extract extracted by a general method can be used. A commercially available papaya extract can also be used in the present invention, and any one or a plurality of commercially available papaya extracts can be used in combination.

In the present invention, the papaya extract can be contained, for example, in an amount of 0.1 to 90% by weight, preferably 1 to 50% by weight, based on the total amount of the oral care composition.

<Other ingredients>
In addition to the above active ingredients, ingredients (materials) that can be blended in ordinary oral care compositions can be further blended into the oral care composition of the present invention as long as the effects of the present invention are not impaired. .. Specifically, for example, in the case of a tablet, an excipient, a lubricant, a binder, a disintegrant and the like can be blended. Furthermore, nutrients such as vitamins, minerals, lactic acid bacteria, and dietary fiber can be blended, and flavorings, acidulants, seasonings, coloring agents, and the like can also be blended. In some cases, various sugars can be blended, and fruit juice, dairy products, and the like can also be blended from the viewpoint of adjusting the taste and sweetness.

Excipients include, for example, saccharides such as lactose, sorbitol, erythritol, D-mannitol, xylitol, maltitol, synthetic aluminum silicate, sodium hydroxypropyl starch, crystalline cellulose, hydroxypropyl starch, anhydrous calcium hydrogen phosphate, reduction. Examples include maltose maltitol.

Examples of the lubricant include sucrose fatty acid ester, glycerin fatty acid ester, magnesium stearate and the like.

Examples of the binder include starch, gum arabic, hydroxypropyl cellulose and the like.

Examples of the disintegrant include celluloses and the like.

<Form>
The oral care composition of the present invention can be appropriately prepared in various forms such as paste, gel, liquid and solid as needed and used as a general oral care product. Specifically, it can be used as an oral care product such as tooth cream, tooth gel, tooth space, mouthwash, mouth spray, tooth powder, gel, candy, gummies, gum, and tablets. Among them, the oral care composition of the present invention can be suitably used as a tablet (for example, a tablet as a refreshing confectionery).

The above form can be prepared by blending the above-mentioned components (materials) and the like.

The oral care composition of the present invention can be suitably used for removing tongue coating, for example, because it continuously suppresses the growth of bacteria in tongue coating and has an excellent tongue coating removing effect. Moreover, since the composition for oral care of the present invention has an excellent Candida glabrata growth inhibitory action, it can be suitably used for, for example, for suppressing the growth of Candida glabrata.

[Composition for oral care for removing tongue coating]
The composition for oral care for removing tongue coating of the present invention contains at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient. The composition for oral care of the present invention has, as active ingredients, a rosemary extract, a propolis extract, and an Indian mushroom extract having an action of suppressing the growth of Candida spp., Which is a major fungus of tongue coating, and Streptococcus spp. By containing one or more of the substance and the papaya extract having an action of suppressing the growth of Candida spp., The growth of the fungus in tongue coating is continuously suppressed and an excellent tongue coating removing effect is exhibited. In addition, the description about the active ingredient (material), other components (material), the form, etc. in the above-mentioned oral care composition is also applied to the description of the composition here.

[Composition for oral care for suppressing the growth of Candida glabrata]
The composition for oral care for suppressing the growth of Candida glabrata of the present invention contains at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient. do. Since the composition for oral care of the present invention contains at least one rosemary extract, propolis extract, Indian mushroom extract, and papaya extract having an excellent Candida glabrata growth inhibitory action as its active ingredient. , Can be used as an oral care composition for suppressing the growth of Candida glabrata. In addition, the description about the active ingredient (material), other components (material), the form, etc. in the above-mentioned oral care composition is also applied to the description of the composition here.

[Composition for oral care for suppressing growth of Streptococcus mutans]
The composition for oral care for suppressing the growth of Streptococcus mutans of the present invention contains at least one selected from the group consisting of rosemary extract, propolis extract, and Indian mushroom extract as an active ingredient. Since the composition for oral care of the present invention contains at least one rosemary extract, propolis extract, and Indian mushroom extract having an excellent streptococcus mutans growth inhibitory action as its active ingredient, streptococcus mutans. It can be used as an oral care composition for suppressing mutans growth. In addition, the description about the active ingredient (material), other components (material), the form, etc. in the above-mentioned oral care composition is also applied to the description of the composition here.

The present invention will be specifically described with reference to the following examples, but the present invention is not limited to the examples.

[Antibacterial test 1]
In this test, various test substances are cultured in a liquid medium together with the target bacteria (Candida glabrata or Streptococcus mutans), and the growth of the bacteria is measured after 4 hours to suppress the growth of the various test substances against each of the bacteria. The effect was evaluated.

(Test substance)
The test substances were rosemary extract, propolis extract, Indian mushroom extract, papaya extract, amla extract, holi basil extract, adhatoda vasica extract, grape seed extract, curcumin, tetrahydrocurcuminoid, green tea extract, Gymnema sylvestre extract, cranberry extract, Tsurumurasaki extract, and olive lees extract were used.

(YM medium)
As a medium for culturing Candida glabrata, a YM medium (liquid / solid) having the following composition was used.

Glucose (wako) 10g
Peptone (Nihon Pharmaceutical) 5g
Yeast extract (DIFCO) 3g
Malt extract (Biokar) 3g
Ultrapure water 1L
Agar (wako) 15g (in the case of solid)

(TBS medium)
As a medium for culturing Streptococcus mutans, a TBS medium (liquid / solid) having the following composition was used. The pH was adjusted to 7.1 before use.

Trypto casein soy broth (Biokar) 30g
Yeast extract (DIFCO) 3g
Ultrapure water 1L
Agar (wako) 15g (in the case of solid)

<Test method>
(1) Preparation of diluted bacterial solution Bacteria (Candida glabrata or Streptococcus mutans) are inoculated on a solid medium and placed in an incubator (MCO-96, manufactured by SANYO) at 37 ° C. and 5% CO ₂ . It was statically cultured in the evening. The cultured cells were inoculated into each liquid medium (10 mL) and cultured overnight before standing at 37 ° C. and 5% CO ₂ . The preculture solution was measured at OD630 using a plate reader (MTP-800, manufactured by Corona Electric Co., Ltd.) and diluted with a liquid medium so that OD630 became 0.1. The obtained diluted bacterial solution was subjected to the test.

(2) Preparation of test solutions Diluted series of various test substances were prepared. First, the test substance was collected in a 1.5 mL tube (manufactured by Watson), and a reference solution (100 mg / mL) was prepared using ultrapure water or DMSO. Dilution series (1, 10, and 100 mg / mL) were prepared by repeating 10-fold dilution with respect to this reference solution. The obtained dilution series was used as a test solution for the test. For papaya extract, dilution series (100, 500, and 1000 mg / mL) were also prepared.

(3) Antibacterial test Various test substances were subjected to antibacterial test according to the following procedure. First, the diluted bacterial solution (198 μL / well) was dispensed into a 96-well plate (IWAKI), and then each of the above test solutions (2 μL / well) was dispensed and lightly mixed by pipetting. Thus, the test substance was subjected to a final concentration of 0, 10, 100, 1000 μg / mL (for papaya extract, a final concentration of 0, 1000, 5000, 10000 μg / mL during the antibacterial test against Candida glabrata). A diluted bacterial solution containing was prepared (where 0 μg / mL was dispensed with ultrapure water or DMSO instead of the test solution). This was statically cultured in an incubator under the conditions of 37 ° C. and 5% CO ₂ for 4 hours, and then OD630 was measured using a plate reader. The amount of change in OD630 (growth rate) at each final concentration was compared with the amount of change in OD630 after 4 hours of culture in the diluted bacterial solution containing the test substance (final concentration 0 μg / mL) as a reference (growth rate 100%). By doing so, the inhibitory effect of each test substance on bacterial growth was evaluated.

(result)
FIG. 1 shows the results of the antibacterial test 1. FIG. 1A is a graph showing the cell growth rate of Candida glabrata at each concentration of the test substance, and FIG. 1B is a graph showing the cell growth rate of Streptococcus mutans. Statistical analysis was performed by the Dunnett multiple comparison method, and the significance levels were set to 5% (*) and 1% (**) in all two-sided tests. As shown in FIG. 1, rosemary extract, propolis extract, Indian mushroom extract, and papaya extract showed a significant growth inhibitory effect on Candida glabrata. In addition, rosemary extract, propolis extract, and Indian mushroom extract also showed a significant growth inhibitory effect on Streptococcus mutans. For other test substances, the green tea extract only showed a growth inhibitory effect on Streptococcus mutans (data not shown). From these results, it was clarified that the rosemary extract, the propolis extract, the Indian mushroom extract, and the papaya extract exert an excellent growth inhibitory effect on the fungus in tongue coating.

[Antibacterial test 2]
In this test, various test substances were cultured in a liquid medium together with the target bacteria (Candida glabrata or Streptococcus mutans), and then seeded in a solid medium (plate medium) under the conditions of 37 ° C. and 5% CO ₂ . Below, Candida glabrata was allowed to stand for 17 hours and Streptococcus mutans for 43 hours, and the number of colonies formed was counted. Using this number of colonies as an index, the inhibitory effect of various test substances on bacterial growth was evaluated.

(Test substance)
The test substances used were rosemary extract, propolis extract, Indian mushroom extract, and papaya extract (each extract used was the same as in antibacterial test 1).

(Culture medium)
The same YM medium as in Antibacterial Test 1 was used as the medium for culturing Candida glabrata. The same TBS medium as in Antibacterial Test 1 was used as the medium for Streptococcus mutans culture.

<Test method>
(1) Preparation of diluted bacterial solution Bacteria (Candida glabrata or Streptococcus mutans) are inoculated on a solid medium and used in an incubator (MCO-96, manufactured by SANYO) under the conditions of 37 ° C. and 5% CO ₂ . It was statically cultured overnight. The cultured cells were inoculated into a liquid medium (10 mL) and cultured overnight at 37 ° C. and 5% CO ₂ before standing. By diluting the preculture with a liquid medium, a diluted bacterial solution of approximately 1000 cells / mL was obtained and used for the test.

(2) Preparation of test solution The test substance was collected in a 1.5 mL tube (manufactured by Watson), and a test solution having a concentration of 300 mg / mL of the test substance was prepared using ultrapure water or DMSO. The obtained test solution was subjected to the test.

(3) Antibacterial test Various test substances were subjected to antibacterial test according to the following procedure. First, the diluted bacterial solution (90 μL) was dispensed into a 1.5 mL tube, and then the test solution (10 μL) was dispensed and lightly mixed by pipetting. At this time, for the control, ultrapure water or DMSO was dispensed instead of the test solution. This was statically cultured in an incubator under the conditions of 37 ° C. and 5% CO ₂ for 30 minutes, and then the whole amount was inoculated on a solid medium (plate medium). This was statically cultured under the conditions of 37 ° C. and 5% CO ₂ , and the number of colonies formed on the medium was counted. The relative value of the number of colonies in each test solution when the number of control colonies was used as a reference (100%) was calculated to evaluate the inhibitory effect of each test substance on bacterial growth.

(result)
FIG. 2 shows the results of the antibacterial test 2. FIG. 2A is a graph showing the colony formation rate of Candida glabrata, and FIG. 2B is a graph showing the colony formation rate of Streptococcus mutans. As shown in FIG. 2, the rosemary extract, the propolis extract, the Indian mushroom extract, and the papaya extract showed an excellent growth inhibitory effect on Candida glabrata. In addition, rosemary extract, propolis extract, and Indian mushroom extract also showed an excellent growth inhibitory effect on Streptococcus mutans. From this result, it was clarified that the rosemary extract, the propolis extract, the Indian mushroom extract, and the papaya extract exert an excellent growth inhibitory effect on the fungus in tongue coating.

[Bacterial suppression test in tongue coating when each active ingredient is ingested alone]
After having the subject ingest each of the four active ingredients of the present invention (rosemary extract, propolis extract, Indian mushroom extract, and papaya extract) independently, the number of bacteria in tongue coating collected from the back of the tongue was counted. By measuring, the effect of suppressing bacteria in tongue coating when each active ingredient was taken alone was evaluated. The same extract as in Antibacterial Test 1 was used.

<Test method>
The subject (healthy adult male) was asked to put 200 mg of the active ingredient on the tongue, move the tongue for about 30 seconds so that it would be applied to the entire back of the tongue, and then swallow it. Immediately before and immediately after ingestion, the number of bacteria in tongue coating was measured according to the following measurement method. The rate of change in the number of bacteria was calculated based on the number of bacteria immediately before ingestion (100%), and the effect of suppressing the bacteria in tongue coating was evaluated.

(Measuring method)
A bacterial counter (DU-AA01NP-H, manufactured by Panasonic Healthcare Co., Ltd.) was used to measure the number of bacteria in tongue coating. Specifically, a cotton swab (manufactured by Heiwa Medic Co., Ltd.) is placed on the center of the back of the tongue, and tongue coating is collected by making three round trips with a width of 1 cm in the front-back direction. Was measured. Hereinafter, the number of bacteria in tongue coating was measured by the same method.

(result)
FIG. 3 shows the results of a bacterial suppression test in tongue coating when each active ingredient was ingested alone. As shown in FIG. 3, the number of bacteria in the tongue coating immediately after ingestion was significantly reduced as compared with that immediately before ingestion. From this result, it was clarified that the rosemary extract, the propolis extract, the Indian mushroom extract, and the papaya extract show an excellent growth inhibitory effect on the fungus in tongue coating even when taken alone.

[Bacterial suppression effect test in tongue coating and tongue coating removal effect test]
In this test, a tablet 1 (invention product) containing the three active ingredients of the present invention (rosemary extract, propolis extract, and papaya extract) was licked by a subject without chewing and collected from the back of the tongue. By measuring the number of bacteria in tongue coating and visually observing the removal of tongue coating, the effect of suppressing bacteria in tongue coating and the effect of removing tongue coating of Tablet 1 were evaluated. In addition, as a reference, two types of commercially available oral care tablets (tablet 2 and tablet 3) were also evaluated in the same manner.

(Tablet 1)
As shown in Table 1, the three active ingredients of the present invention, excipients and the like were mixed to prepare a powder for tablets. Here, the rosemary extract and the propolis extract are water-insoluble, and the papaya extract is water-soluble (same as used in the antibacterial test). As excipients, lubricants and fragrances, materials generally used for tablets were used.

The prepared powder was put into a tableting machine (HANDTAB-100, manufactured by Ichihashi Seiki Co., Ltd.), pressed to 20 kN, and locked to produce Tablet 1. The prepared tablets were stored in a dark place in a closed container containing a desiccant until the test.

(Tablets 2 and 3)
Two types of commercially available oral care tablets were prepared as Tablet 2 and Tablet 3. The tablet 2 is a composition focusing on the removal of the biofilm generated by the bacteria on the surface of the tongue. Further, the tablet 3 is a composition focusing on the removal of tongue coating.

<Test of bacterial suppression effect in tongue coating when ingesting tablets>
The subjects (healthy adult males / 6 people) licked Tablet 1 (1 tablet) without chewing until it was completely melted. Immediately before ingestion, immediately after ingestion, and 4 hours after ingestion, the number of bacteria in tongue coating was measured according to the above-mentioned measurement methods. The same applies to tablets 2 and 3 (however, 4 and 5 subjects, respectively). The rate of change in the number of bacteria was calculated based on the number of bacteria immediately before ingestion (100%), and the effect of suppressing the bacteria in tongue coating was evaluated. In each subject, the number of bacteria in the tongue coating immediately before ingestion was 3 million or more.

(result)
FIG. 4 shows the results of a bacterial suppression test in tongue coating when tablets 1 (invention product), tablets 2 and 3 (predecessor products) were ingested. As shown in FIG. 4, the number of bacteria in tongue coating was significantly reduced in tablet 1 immediately after ingestion and 4 hours after ingestion as compared with immediately before ingestion, and even when compared with tablets 2 and 3. It showed a more remarkable bacterial suppression effect. In particular, the effect of Tablet 1 (invention product) was maintained even after 4 hours of ingestion (bacterial count decrease rate 90%), and even when compared with immediately after ingestion (bacterial count decrease rate 80%), it was contained in tongue coating. The number of bacteria was further reduced. On the other hand, in tablets 2 and 3 (predecessor product), the effect of suppressing bacteria on tongue coating was observed immediately after ingestion, but after that, the number of bacteria started to increase, and the effect was not sustained.

FIG. 5 shows photographs of the tongue immediately before, immediately after, and 4 hours after ingestion of tablet 1. It can be confirmed that the tongue coating was effectively removed by ingesting the tablet 1.

<Sustainable test (effect of suppressing bacteria in tongue coating when taking Tablet 1)>
The subject (healthy adult male) was asked to lick Tablet 1 (1 tablet) without chewing after brushing his teeth before going to bed until it was completely melted, and before and after that, the number of bacteria in tongue coating was measured. Specifically, after brushing teeth before going to bed (immediately before taking the tablet), before going to bed (immediately after taking), when waking up the next day (7 hours after taking), before lunch (12 hours after taking), before dinner (19 hours after taking) The number of bacteria was measured at the time of waking up the next day (33 hours after ingestion). The rate of change in the number of bacteria was calculated based on the number of bacteria immediately before ingestion (100%), and the effect of suppressing the bacteria in tongue coating was evaluated. During the measurement period, they lived as usual without any particular restrictions, except that they were prohibited from taking other oral care products. For reference, the same subject was subjected to bacterial count measurement even when Tablet 1 was not ingested (only after brushing teeth before going to bed and waking up the next day).

(result)
FIG. 6 shows the results of the continuous test. As shown in FIG. 6, the number of bacteria in the tongue coating after ingestion of Tablet 1 was significantly reduced as compared with immediately before ingestion, and the rate of decrease in the number of bacteria was maintained at 90% or more until before dinner (19 hours later) the next day. The bacterial suppression effect was maintained until the next day when the patient woke up (33 hours later: 80% decrease in bacterial count). On the other hand, when the tablet was not ingested, the number of bacteria in the tongue coating increased when waking up the next day as compared with before going to bed. From this result, it was clarified that Tablet 1 is excellent in the effect of suppressing bacteria in tongue coating and the sustainability of the effect.

<Observation of tongue coating removal>
An appropriate amount of dyed gel (Plaque Check Gel BR, manufactured by GC Corporation) was taken on a cotton swab (manufactured by Heiwa Medic Co., Ltd.) and applied to the entire back of the tongue of the subject (healthy adult male). After rinsing the mouth with water to remove excess gel, the subject was asked to lick Tablet 1 or 3 (1 tablet each) until it was completely dissolved without chewing. The degree of tongue coating removal was visually evaluated using tongue photographs before and after ingestion, and the staining (bleaching) level before and after ingestion was used as an index. Photography was performed before staining, after staining (before ingestion), and immediately after ingestion.

In addition, in order to compare the removal effect, the effect of cleaning with a tongue brush (4 minutes) was also verified by the same test.

(result)
FIG. 7 is a tongue photograph showing the level of tongue coating removal during ingestion of tablets 1 and 3 or cleaning of a tongue brush. It can be seen that the tongue coating is stained and visualized by the dyeing gel (see "After dyeing"). Comparing after ingestion (or after cleaning), it can be confirmed that the staining level is significantly reduced (tongue coating is removed) by ingestion of tablet 1. Almost no stained part was seen on the tongue side and tip of the tongue, and only a little remained on the back of the tongue. In the tablet 3, the staining level is partially lowered, and a large amount of stained portion (tongue coating) still remains. In the tongue brush cleaning, the staining level is uniformly lowered (tongue coating is removed), but it is usually not used for 4 minutes and may hurt the tongue, so it can be said that it is difficult to repeat it every day. From these facts, it was shown that ingestion of tablet 1 is a useful means for efficiently removing tongue coating.

It has been clarified that the tablet of the present invention continuously suppresses the growth of bacteria in tongue coating and exhibits an excellent tongue coating removing effect.

An example of prescribing the oral care composition of the present invention is shown below. The blending ratio is% by weight.

<Prescription example 1: Tablet (cool confectionery)>
Rosemary extract 30%
Propolis extract 1%
Papaya extract 20%
Sorbitol 19%
Erythritol 18%
Magnesium stearate 2%
Fragrance 10%
100% in total

<Prescription example 2: Candy (sugar confectionery)>
Indian mushroom extract 15%
Propolis extract 4%
Papaya extract 15%
50% sugar
10% starch syrup
Fragrance 6%
100% in total

<Prescription example 3: Gum (chewing gum)>
Indian mushroom extract 10%
Rosemary extract 10%
Papaya extract 20%
Gum base 24%
Xylitol 20%
Maltitol 15%
Fragrance 1%
100% in total

<Prescription example 4: Tooth paste (toothpaste)>
Indian mushroom extract 5%
Rosemary extract 8%
Propolis extract 1%
Papaya extract 10%
Anhydrous calcium hydrogen phosphate 30%
Xylitol 20%
Glycerin fatty acid ester 5%
Xanthan gum 20%
Fragrance 1%
100% in total

The composition for oral care of the present invention continuously suppresses the growth of bacteria in tongue coating and exhibits an excellent tongue coating removing effect. Such an oral care composition is useful as an oral care product such as, for example, a tablet (for example, a tablet as a refreshing confectionery).

Claims (9)

An oral care composition containing at least two selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient. The oral care composition according to claim 1, wherein one or more selected from the group consisting of rosemary extract, propolis extract, and Indian mushroom extract; and papaya extract as an active ingredient. The oral care composition according to claim 1 or 2, which contains a rosemary extract, a propolis extract, and a papaya extract as active ingredients. The composition for oral care according to any one of claims 1 to 3, wherein the total content of the rosemary extract, the propolis extract, and the Indian mushroom extract is 10 to 90% by weight in the total amount of the composition. One of claims 2 to 4, wherein the weight ratio of the total content of the rosemary extract, the propolis extract, and the Indian mushroom extract to the content of the papaya extract is 1: 9 to 9: 1. The composition for oral care according to. An oral care composition for removing tongue coating, which comprises at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient. The oral care composition according to any one of claims 1 to 6, which is a tablet. A composition for oral care for suppressing the growth of Candida glabrata, which contains at least one selected from the group consisting of rosemary extract, propolis extract, Indian mushroom extract, and papaya extract as an active ingredient. A composition for oral care for suppressing the growth of Streptococcus mutans, which comprises at least one selected from the group consisting of rosemary extract, propolis extract, and Indian mushroom extract as an active ingredient.

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