JP2022053321A - Culture solution of yeast, external skin preparation containing the culture solution, skin cosmetics containing the external skin preparation, and method for producing the culture solution - Google Patents

Abstract

To maintain or improve skin conditions, e.g. by improving the texture of skin or moisturizing the skin.SOLUTION: A culture solution, in which yeast has been grown in a medium containing white birch sap, is used to maintain or improve skin conditions.SELECTED DRAWING: None

Description

The present invention relates to a culture solution of yeast that can be used as an external preparation for skin or a skin cosmetic, an external preparation for skin containing the culture solution, a skin cosmetic containing the culture solution, and a method for producing the above culture solution.

Aging care and anti-aging are matters of great interest to both women and men, and various products dedicated to them such as cosmetics and foodstuffs are on the market. It is said that when the amount of sebum and water in the skin decreases with aging, the moisturizing power of the stratum corneum on the skin surface is lost, and fine wrinkles and dry skin are likely to occur. In addition, due to the influence of air conditioning, the skin is exposed to dryness all year round, and there is increasing interest in skin external preparations and skin cosmetics that have high moisturizing and aging care effects.

Conventionally, skin cosmetics have a moisturizing effect on the skin by blending polyhydric alcohols such as glycerin, hyaluronic acid, ceramide, polysaccharides, yeast extract, yeast culture solution, moisturizers such as jojoba oil, and oil components. Many attempts have been made to improve the skin's appearance and make the skin glossy. The external skin preparation also contains oily components such as white petrolatum, liquid paraffin, isopropyl myristate, beeswax, lanolin, and stearic acid as a base other than the main ingredient. The base for external skin preparations not only enhances the medicinal effect of the main agent, but also contributes to the protective effect and moisturizing effect of the skin.

Specifically, among skin cosmetics, for aging care cosmetics, many anti-aging compositions have been proposed from various angles for improving wrinkles, dullness, slack, etc. of the skin condition deteriorated with aging. (See Patent Documents 1 to 6).

By the way, white birch is native to the temperate northern part of East Asia, and grows mainly in cool and natural areas such as Hokkaido. Filters of sap and the like have been proposed as raw materials for cosmetics. For example, in Patent Document 7 and Patent Document 8, a whitening composition that exhibits an effect of improving arteriosclerosis and cataract caused by aging and a whitening effect on a birch extract extracted from a birch bark with a hydrophilic solvent such as ethanol. The thing is shown. Further, Patent Document 9 proposes a blended cosmetic containing gel fine particles containing a birch sap component, and Patent Document 10 proposes a bath agent and a foot bath agent containing birch sap as a component.

On the other hand, there are many advanced technologies for using yeast culture solutions and extracts extracted from cultured yeast for external skin preparations and skin cosmetics, and many compounded products are sold to general consumers. For example, Patent Document 11 describes a yeast extract for cosmetics, which is obtained by culturing yeast in a medium having a high content of glutamic acid, cysteine and glycine, without digesting with enzymes or proteases in the cells. Yeast extracts extracted with glutamic acid are disclosed. Patent Document 12 discloses a composition having a heat shock protein 70 production-inducing effect by using a yeast cell extract in which a zinc compound is accumulated in a cell at a high concentration. Patent Document 13 discloses a culture in which yeast is cultured in a medium containing hyaluronic acid selected from the group consisting of hyaluronic acid, hyaluronic acid derivatives and salts thereof, cosmetics containing the same, and external skin products. There is. Patent Document 14 discloses a fermentation broth using nicotinamide mononucleotide and yeast, which has an excellent moisturizing effect and texture improving effect and is less irritating.

The culture medium for culturing yeast contains carbon sources such as glucose or other sugars, nitrogen sources such as yeast extract, peptone and corn steep liquor, and minerals such as potassium and calcium as substrates. Patent Documents 15 and 16 disclose that a concentrated solution or a dried product of a culture solution in which yeast is cultured in a medium containing glucose, skim milk and yeast extract as substrate components is used as a skin pharmaceutical agent or cosmetic. .. Further, in Patent Document 17, a soymilk fermented product fermented with yeast using soymilk as a substrate component is used as a blast cell proliferation agent, an epidermal keratinocyte proliferation agent, a collagen production promoter, a hyaluronic acid production promoter, and a cosmetic. Is disclosed.

Japanese Unexamined Patent Publication No. 2019-203033 Japanese Unexamined Patent Publication No. 2007-291102 Japanese Unexamined Patent Publication No. 2008-169196 Japanese Unexamined Patent Publication No. 2008-255020 Japanese Unexamined Patent Publication No. 2008-260721 Japanese Unexamined Patent Publication No. 2009-040690 Japanese Unexamined Patent Publication No. 10-15244 Japanese Unexamined Patent Publication No. 2001-335798 Japanese Patent Application Laid-Open No. 2003-321323 Japanese Unexamined Patent Publication No. 2006-131546 Japanese Unexamined Patent Publication No. 2006-347988 Japanese Unexamined Patent Publication No. 2014-108945 JP-A-2015-221756 Japanese Unexamined Patent Publication No. 2018-145167 Special Publication No. 61-43033 Special Fair 3-48166 Gazette Japanese Unexamined Patent Publication No. 2017-155029

However, it cannot be said that the birch sap has a sufficient moisturizing and texture-improving effect in terms of its use as an external skin preparation or skin cosmetic. Further, the technique of culturing yeast in a medium containing birch sap as a substrate has not been known, and it has been completely unclear what kind of action and effect will be exerted on the culture medium in which yeast is cultured. Therefore, in view of the actual situation, the present invention clarifies how an excellent effect is obtained on a yeast culture solution using birch sap, whereby the yeast culture solution having the excellent effect and the culture thereof. It is an object of the present invention to provide a skin external preparation containing a liquid, a skin cosmetic containing the culture liquid, and a method for producing the culture liquid.

As a result of diligent studies by the present inventors, when yeast was cultured in a medium containing birch sap, the obtained culture solution had an action effect of maintaining and improving the skin condition such as a texture improving effect and a moisturizing effect. We found it and came to complete the present invention. The present invention includes the following.

(1) A culture medium in which yeast is grown in a medium containing birch sap.
(2) The culture solution according to (1), wherein the birch sap is a sap obtained from Betula platyphylla.
(3) The yeast is characterized by being one yeast selected from the group consisting of yeast belonging to the genus Saccharomyces, yeast belonging to the genus Saccharomyces and yeast belonging to the genus Saccharomyces, or a plurality of yeasts selected from the group. The culture solution according to (1) or (2).
(4) An external skin preparation containing the culture solution according to any one of (1) to (3) above.
(5) A skin cosmetic containing the culture solution according to any one of (1) to (3) above.
(6) The skin cosmetic according to (5), which exhibits at least one effect selected from the group consisting of a moisturizing effect, a texture improving effect, and a dullness improving effect.
(7) During the process of culturing yeast in a medium containing birch sap, the small molecule sugar in the birch sap is consumed as a nutrient source for yeast, and the small molecule sugar in the birch sap is lower than before the start of culturing. A method for producing a culture medium, which is characterized by having a concentration.

The culture solution according to the present invention can maintain and improve the condition of the skin such as the texture improving effect and the moisturizing effect on the skin. In particular, the culture broth according to the present invention is extremely excellent in the effect of maintaining and improving the condition of the skin as compared with the birch sap in which yeast is not cultivated.

Since the external skin preparation and the skin cosmetic according to the present invention contain the culture solution, it is possible to maintain and improve the condition of the skin such as the texture improving effect and the moisturizing effect. Further, the method for producing a culture solution according to the present invention can produce a culture solution having a low concentration of low molecular weight sugar, which is a culture solution capable of maintaining / improving the skin condition such as a texture improving effect and a moisturizing effect. ..

It is a characteristic figure which shows the result of having measured the glucose concentration with respect to Production Examples 1 to 4 produced in Example 1. FIG.

Hereinafter, the present invention will be described in detail.

The culture medium to which the present invention is applied is a culture medium in which yeast is cultured in a medium containing birch sap. Here, the birch sap means the sap collected from the birch. Birch is a broadly defined birch (scientific name: Betula platyphylla, synonim: Betula pendula var. Platyphylla), American birch (scientific name: Betula papyrifera), Oshuushira kamba (scientific name: Betula pendula, alias: Sidarekamba), European name. Betula pubescens, also known as European birch (also known as European birch), and birch (scientific name: Betula szechuanica, synonim: Betula pendula var. Szechuanica) can be mentioned. Among them, as the birch sap, the sap collected from the birch classified into the broadly defined birch (scientific name: Betula platyphylla) is preferable. Birch classified into broadly defined birch (scientific name: Betula platyphylla) includes Kouan birch (scientific name: Betula platyphylla var. Platyphylla), narrowly defined birch (scientific name: Betula platyphylla var. . japonica f. Laciniata), Ezonoshirakamba (scientific name: Betula platyphylla var. Kamtschatica) and Karafuto birch (scientific name: Betula platyphylla var. Mandshurica) can be mentioned.

The birch sap may be sap collected from one of the above-mentioned birch types, or may be a mixture of sap collected from a plurality of types of birch. The birch sap is collected, for example, by making a hole in the trunk of the birch tree or making a linear notch. The sap to be collected may have different components depending on the habitat and season of the tree. For example, the sap to be collected is about 99% water, and the remaining solid content is monosaccharides such as glucose and fructose, and the mineral content is potassium, sodium, calcium, magnesium, manganese, iron, zinc, phosphorus, barium, and silicon. , Boron, lanthanum, strontium, barium, aluminum, chromium, etc. In addition, birch sap has other amino acid components such as glutamic acid, aspartic acid, valine, isoleucine, methionine, leucine, lysine, phenylalanine, glycine, alanine, threonine, arginine, cystine cysteine, proline, serine, tyrosine, and histidine. , Organic acids such as aspartic acid and succinic acid, proteins, polysaccharides and the like may be contained.

The yeast to be cultured in a medium containing birch sap is not particularly limited, and yeast disclosed in The Yeasts, Fifth Edition: A Taxonomic Study and the like can be used. Examples of yeasts include yeasts of the genera Candida, Galactomyces, Saccharomycopsis, Saccharomyces, and Pichia. In particular, one type of yeast may be cultivated in a medium containing birch sap, but a plurality of yeasts may be used simultaneously or at different times for culturing.

In particular, among yeasts, the yeasts of the genus Galactomyces include, for example, Galactomyces reessii, Galactomyces geotrichum, Galactomyces candidus, and Galactomyces pseudocandidas. It is preferable to use pseudocandidus) or the like. Among yeasts, examples of yeasts belonging to the genus Saccharomycopsis include Saccharomycopsis capsularis, Saccharomycopsis crataegensis, Saccharomycopsis malanga, and the like. It is preferable to use it. Further, among the yeasts, as the Saccharomyces genus yeast, for example, Saccharomyces cerevisiae, Saccharomyces bacillaris and the like are preferably used. Further, as the yeast of the genus Saccharomyces, commonly used brewer's yeast, sake yeast, wine yeast, baker's yeast and the like may be used. As for the yeasts listed as examples, one kind may be used alone, or two or more kinds of different yeasts may be used in combination.

The birch sap contained in the yeast medium may be in any form, and for example, a squeezed undiluted solution may be used, or a coarsely filtered product of the undiluted solution may be used. The birch sap to be used is preferably one obtained by filtering to remove contaminants, impurities and insoluble solids. Further, the birch sap may be a stock solution diluted to a predetermined magnification with a solvent such as water, or may be concentrated to a predetermined magnification. However, as the birch sap, it is most preferable to use the collected component concentration without diluting or concentrating it.

Further, it is preferable that the birch sap is sterilized before culturing the yeast. That is, when birch sap is added to a normal yeast medium and used, the birch sap may be sterilized before the birch sap is added to the medium, or the medium itself to which the birch sap is added may be sterilized. May be processed. The sterilization treatment of the birch sap or the medium containing the birch sap can be performed by any method such as heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, ozone sterilization and the like. For example, the birch sap or the medium containing the birch sap may be sterilized by heating at 65 ° C. or higher, for example, 110 to 130 ° C. or 120 to 130 ° C., and in one example, it can be sterilized by treating at 121 ° C. for 20 minutes. .. After heat sterilization, it is preferable to cool the yeast to a temperature suitable for culturing yeast and then use it for yeast culturing.

Culturing of yeast in a medium containing birch sap can be performed by inoculating the medium with yeast and culturing. Here, the medium may be a single composition of birch sap alone, or may be a mixed composition containing a normal yeast medium and birch sap. A normal yeast medium is a medium containing components (carbon source, nitrogen source, etc.) necessary for yeast culture. A normal yeast medium contains a component selected from yeast extract, peptone, casein, soybean decomposition products, malt extract, amino acids and the like as a nitrogen source required for culturing. In addition, a normal yeast medium contains mineral components such as glucose and the like and sodium salts, potassium salts, calcium salts and magnesium salts as carbon sources. When the birch sap contains a sufficient amount of carbon source and / or mineral component for yeast culture, the yeast medium used in combination with the birch sap has the carbon source and / or mineral component removed from the usual composition. It may be a composition. In particular, the medium suitable for culturing yeast contains birch sap, yeast extract, and peptone.

In culturing yeast in a medium containing birch sap, the amount of sugar as a carbon source contained in the medium is preferably 0.05 to 5 g / 100 ml in terms of glucose, and more preferably 0.1 to 2 g / 100 ml in terms of glucose. .. Here, the birch sap contains about 1% by weight of sugar components such as glucose and xylose. Therefore, the birch sap can be used as it is without diluting and concentrating it. By using the birch sap as it is, it is possible to fully exert the effects of maintaining and improving the skin condition such as the texture improving effect and the moisturizing effect.

The birch sap may be diluted or concentrated to a predetermined magnification before use. By diluting or concentrating, the concentration of carbon source and mineral components contained in the birch sap can be adjusted to a desired range. It is also possible to add sugar such as glucose to the birch sap so that the carbon source is in a desired range. Further, the mineral component can be similarly added to the birch sap so as to have a desired concentration.

When yeast is cultured in a medium containing birch sap, yeast may be directly added (inoculated) to the medium, but it is preferable to add a preculture medium in which yeast is precultured. By adding the preculture medium, the culture time of the main culture can be shortened and the risk of contamination can be reduced. The medium used for the pre-culture may have the same composition as the medium used for the main culture, or a normal medium for yeast that does not contain birch sap may be used. In particular, in this preculture, it is most preferable to use a medium containing birch sap to cover the carbon source and mineral components required for yeast culture.

By performing the pre-culture, it is possible to shift to the main culture while maintaining the yeast in the logarithmic growth phase, so that the production efficiency in the large-scale culture of the yeast can be significantly improved. The culture conditions for yeast are not particularly limited as long as the yeast can grow. The culture temperature may be any temperature as long as the yeast can grow, and is not particularly limited, but is typically 15 to 45 ° C, preferably 25 to 40 ° C, for example 28 to 37 ° C. The pH at the time of culturing may be any pH as long as it can grow yeast, and is not particularly limited, but may be 3.0 to 8.0, preferably 4.0 to 7.0, for example 5.0 to 7.0.

Yeast can convert sugar into carbon dioxide and use it as an energy source under aerobic conditions, and it is known that yeast grows and proliferates well under these conditions. On the other hand, under oxygen-poor conditions, the growth slows down and homo or heterofermentation produces alcohol and carbon dioxide or organic acids. In particular, when culturing yeast in a medium containing birch sap, it is preferable to culture the yeast under aerobic conditions. By culturing under aerobic conditions, it is possible to produce a culture solution having an effect of maintaining / improving the skin condition such as a texture improving effect and a moisturizing effect. In order to aerobically cultivate yeast in a medium containing birch sap, an aeration-stirring fermenter used for ordinary microbial culture can be used. At this time, the aeration amount is not particularly limited, but may be, for example, 0.01 to 5vvm, preferably 0.1 to 2vvm, and more preferably 0.3 to 1.5vvm. Further, the stirring speed of the medium is not particularly limited, and conditions suitable for the growth of yeast can be arbitrarily set.

In addition, substances that change the pH, such as organic acids, may be produced along with the culture of yeast, and the change in pH may inhibit the growth of yeast. For example, when organic acids are accumulated with the cultivation of yeast, the pH of the medium decreases, so the pH should be within the above range, preferably within the pH range of 4.0 to 7.0, for example, 5.0 to 7.0. It is preferable to continue the culture by a method such as adding a pH adjuster to adjust the pH.

Further, the time for culturing yeast in a medium containing birch sap is not particularly limited, but is, for example, 5 hours to 1 month, for example, 1 to 10 days or 1 to 5 days, preferably 1 to 3 days. can do.

As described above, yeast can be cultivated in a medium containing birch sap to produce a culture broth. The obtained culture solution has excellent characteristics such as maintaining and improving the skin condition such as texture improving effect and moisturizing effect. In particular, the obtained culture solution is extremely excellent in the degree of maintaining and improving the skin condition such as the texture improving effect and the moisturizing effect, as compared with the case where the birch sap is used alone. Therefore, the obtained culture solution can be used as a skin external preparation or a skin cosmetic.

In addition, the culture broth obtained by culturing yeast in a medium containing birch sap has a reduced stickiness as compared with birch sap. Therefore, the skin external preparation and the skin cosmetic containing this culture solution can maintain and improve the skin condition such as the texture improving effect and the moisturizing effect, and are more refreshing and comfortable to use.

Further, the obtained culture solution may be used as it is as a skin external preparation or a skin cosmetic, or may be used as a skin external preparation or a skin cosmetic after undergoing a process such as filtration. Further, the obtained culture solution is not limited to the filtration step, and for example, it is preferable to perform a step of exuding the bacterial cell component or the component accumulated in the bacterial cell before the filtration step. This step is not particularly limited, but may be lysed by the action of a digestive enzyme such as protease, lipase, or amylase, or may be decomposed by an acid after separating the bacterial cells, or may be rotated at high speed. Yeast may be physically crushed by means such as crushing, ultrasonic crushing, and freeze-drying crushing, or may be heated for sterilization. By these steps, useful components contained in yeast can be rationally extracted into the culture broth.

As described above, the culture broth obtained by culturing yeast in a medium containing birch sap can be used as it is as an external preparation for skin or skin cosmetics, but the culture broth that has undergone the various steps described above can also be used as it is. It can be used as an external skin preparation or skin cosmetic. Therefore, in the present specification, the term "culture solution" refers to a solution obtained by culturing yeast in a medium containing birch sap, a solution after filtering the culture solution, and the above-mentioned. It also includes solutions after various steps, filtered solutions of the solutions, and other solutions that have been subjected to one or more treatments by centrifugation, deodorization / decolorization with a filtration aid such as activated charcoal or diatomaceous soil, or a combination thereof. .. Further, in the present specification, the "culture solution" includes those which are concentrated, diluted, dried, or microfiltered with respect to these solutions, or those which have been purified by centrifugation or the like. The purification method is not particularly limited, and examples thereof include a method of filtering using a filter such as centrifugal filtration or a membrane filter.

In particular, as described above, it is preferable to heat the culture broth obtained by culturing yeast in a medium containing birch sap under high temperature conditions to exudate useful components from the yeast cells into the culture broth. More specifically, the obtained culture broth is heat-treated at 121 ° C. for 20 minutes or more, and then the obtained extract is centrifuged, filtered (for example, microfiltration), or both. It is possible to obtain a filtrate from which contaminants, bacterial cells, insoluble matter and the like have been removed. Exudation of useful components may be performed by cell disruption using a high-pressure homogenizer, an ultrasonic homogenizer, a high-speed rotary homogenizer, or the like, in addition to the decomposition of heated cells, and a combination of these multiple methods may be used. Further, the solid content such as the cell wall of the bacterial cell may be removed together with impurities and insoluble matter by filtering, for example, filtering with a filter.

The obtained culture solution can be filtered by any method. For example, filtration can be performed using a filter or other filtration device suitable for removing contaminants, cells and / or insoluble matter and the like. Filtration using a filter or other filtration device may include, for example, centrifugal filtration, pressure filtration, suction filtration and the like. The filter used for filtering the culture solution is not particularly limited, and examples thereof include a membrane filter, a glass fiber filter, a cellulose filter, and a sintered filter. One of these filters may be used for filtration, but two or more types of filters may be combined for filtration. More specifically, when a microfiltration filter is used, it is typically less than 1 μm, preferably 0.5 μm or less, more preferably 0.1 μm to 0.5 μm or 0.2 μm to 0.5 μm, such as 0.45 μm, 0.22 μm, or 0.1. A filter having a pore size of μm can be used. Further, after filtering with diatomaceous earth or a pre-filter, filtration with a microfiltration filter may be performed.

The obtained culture solution can be diluted or concentrated by any dilution method or concentration method. When diluting, for example water or any other solvent can be used. As a method for concentrating the culture solution, for example, a heat evaporation concentration method, a freeze concentration method, an ultrafiltration method, a dialysis method, a chromatography method or the like can be used.

Any sterilization or sterilization method can be applied to the sterilization / sterilization method of the obtained culture solution. Examples of the sterilization sterilization method include heat sterilization, filter sterilization, ultraviolet sterilization, radiation sterilization, ozone sterilization and the like.

When the obtained culture solution is dried to obtain a dried product, any drying method can be applied. Examples of the method for drying the culture solution include a freeze-drying method for vacuum freeze-dried products and a heat-drying method such as a spray-drying method. Alternatively, it can be carried out by a decolorization treatment method. Examples of the deodorizing treatment and / or the decolorizing treatment method include a method in which the culture solution is passed through the activated carbon and the odorous component and the adsorbing component are adsorbed on the activated carbon.

The obtained culture broth may be subjected to wintering (dewaxing) treatment. That is, the wax can be removed by cooling the obtained culture solution, precipitating a component (wax) that hardens at a low temperature, and separating the solid and liquid by means such as filtration.

Here, the external skin preparation means a drug containing an ingredient (main drug) effective for diagnosis, treatment or prevention of a human or animal disease, or a base used for the drug. The term "external skin preparation" means, for example, including pharmaceuticals and quasi-drugs specified in the "Act on Securing Quality, Effectiveness, Safety, etc. of Pharmaceuticals, Medical Devices, etc." in Japan. On the other hand, skin cosmetics mean cosmetics used on human skin that are not classified as pharmaceuticals or quasi-drugs. The term "external skin preparation" is meant to include cosmetics used on human skin as stipulated in the "Act on Securing Quality, Effectiveness and Safety of Pharmaceuticals, Medical Devices, etc." in Japan.

The external skin preparation may be of any dosage form, and may be, for example, an ointment, a patch, a pap, a liniment, a lotion, a cream, an extract, a flow extract, a tincture, an elixir, a syrup, etc. Examples thereof include limonade agents, aromatic water agents, liquid agents, suspending agents, emulsions, medicated bathing agents, medicated shampoos, aerosol agents, powders, granules, tablets, capsules and the like.

Further, the skin external preparation and the skin cosmetic may be a composition in any form such as a solution, a suspension, an emulsified, a powder, a paste, a mousse, or a gel. In particular, the skin cosmetic may preferably be in any form that can be used in contact with the skin, specifically, for example, a cleanser, a milky lotion, a beauty liquid, a general cream (face cream, hand cream, etc.). Body cream etc.), wash pigment (cleansing cream etc.), pack, shaving cream, sun cream, sunscreen cream, sunscreen lotion, sunscreen lotion, makeup soap, foundation, grate, powder, lipstick, lip cream, eyeliner , Eye cream, eye shadow, mascara, bathing agent (bath agent), shampoo, hair rinse, hair treatment, hair pack, scalp care agent, body rinse, body gel, hair dye, cosmetics for hair and the like. Toners, milky lotions, beauty essences, face creams, facial cleansers and the like are particularly preferable.

Further, in skin external preparations and skin cosmetics, the above-mentioned culture solution can be blended in any form, for example, it may be simply mixed with other components as it is, or it may be in the form of a gel or powder. It can be blended in the form of granules or capsule inclusion bodies.

In addition to the above-mentioned culture solution, the skin cosmetic may contain additives permitted in the cosmetic, and if necessary, other active ingredients and the like. As such additives and other active ingredients, those suitable for the intended use and form can be arbitrarily selected and used. The skin cosmetic can be produced according to any cosmetic production method, for example, by mixing, stirring, molding, filling or the like of raw materials.

The external skin preparation can be a composition containing the above-mentioned culture solution as a main agent for maintaining and improving the skin condition such as texture improving effect and moisturizing effect. Alternatively, the external skin preparation may be a composition containing a predetermined medicinal ingredient (main agent) and containing the above-mentioned culture solution as a base for maintaining and improving the skin condition such as a texture improving effect and a moisturizing effect. The external skin preparation can contain the above-mentioned culture solution, pharmaceutically acceptable additives, and if necessary, other active ingredients. As such additives and other active ingredients, those suitable for the intended use and form of the pharmaceutical can be arbitrarily selected and used. The pharmaceutical product according to the present invention can be produced by mixing, stirring, molding, filling and the like of raw materials according to any pharmaceutical production method.

External skin preparations and cosmetics include carriers (solid or liquid carriers), excipients, surfactants, binders, disintegrants, lubricants, odorants, solubilizers, suspensions, coatings, pH. It may contain one or more pharmaceutical additives such as regulators, colorants, fragrances, flavoring agents, cooling agents, stabilizers, foaming agents, preservatives and buffers.

The external skin preparation and the skin cosmetic may contain, for example, one or two or more components arbitrarily selected from any of the following groups. Proteins such as collagen, collagen hydrolyzate, gelatin, gelatin hydrolyzate, elastin, elastin hydrolyzate, lactoferrin, keratin, keratin hydrolyzate, casein, albumin, royal jelly-derived protein hydrolyzate, honey-derived protein hydrolyzate, etc. And any component selected from protein hydrolysates. Natural highs of sodium hyaluronate and hyaluronic acid derivatives, xanthan gum, carrageenan, guar gum, alginic acid and its salts, pectin, chondroitin sulfate and its salts, water-soluble chitin, chitosan derivatives and their salts, purulan, deoxyribonucleic acid, arabic rubber, tragant rubber and the like. It can contain any component selected from molecules and derivatives thereof. It can contain any ingredient selected from crude drugs such as physalis, luo han guo, jujube, adlay, licorice, eucommia, ginger, and udo. It can contain any component selected from seaweed powder, seaweed extract, and seaweed-derived polysaccharides. It can contain any ingredient selected from animal-derived products such as placenta extract. It can contain any component selected from water such as carbonated water, hot spring water, micro-nanobubble water, purified water, and distilled water. Among carboxyvinyl polymers and salts thereof, polyacrylic acid and salts thereof, acidic polymers such as carboxymethyl cellulose and salts thereof, polyvinyl alcohol, hydroxyethyl cellulose, hydroxypropyl cellulose, methyl cellulose, polyethylene glycol, polyvinylpyrrolidone, nitrocellulose polyvinylmethyl ether and the like. It can contain any component selected from sex polymers. It can contain any component selected from cationic polymers such as cationized cellulose, polyethyleneimine, cationized guar gum and the like. It can contain any component selected from lower alcohols such as ethanol and isopropyl alcohol. It can contain any component selected from UV absorbers such as paraaminobenzoic acid-based UV absorbers, salicylic acid-based UV absorbers, cinnamic acid-based UV absorbers, anthraniere acid-based UV absorbers, and benzophenone-based UV absorbers. .. It can contain any component selected from anti-inflammatory agents such as glycyrrhizic acid and its salts, guaiazulene and its derivatives, and allantoin. It can contain any component selected from antioxidants such as stearic acid ester, nordihydroguaselethenic acid, dibutylhydroxytoluene, butylhydroxyanisole, parahydroxyanisole, propyl gallate, sesamol, sesamolin, gossypol and the like. Contains any ingredient selected from parabenzoic acid esters such as methyl parabenzoate, ethyl parabenzoate, propyl parabenzoate, butyl parabenzoate, and preservatives such as sorbic acid, dehydroacetic acid, phenoxyethanol, and benzoic acid. Can be done. It can contain any component selected from edetic acids such as edetic acid and disodium edetate and salts thereof, and sequestrants such as phytic acid and hydroxyethandisulfonic acid. It can contain any component selected from polyhydric alcohols such as glycerin, 1,3-butylene glycol and propylene glycol. It can contain any component selected from amino acids such as L-aspartic acid, DL-alanine, L-arginine, L-cysteine, L-glutamic acid, glycine and salts thereof. It can contain any component selected from sugars such as maltitol, sorbitol, xylobiose, N-acetyl-D-glucosamine, honey and the like. It can contain any component selected from bases such as aqueous ammonia, monoethanolamine, triethanolamine, sodium hydroxide, potassium hydroxide and the like. It can contain any component selected from hydrocarbons such as liquid paraffin, squalane and petrolatum. It can contain any component selected from fats and oils such as coconut oil, evening primrose oil, jojoba oil, castor oil, and hardened castor oil. It can contain any component selected from fatty acids such as lauric acid, myristyl acid, palmitic acid, stearic acid and behenic acid. It can contain any component selected from higher alcohols such as myristyl alcohol, cetanol, cetostearyl alcohol, stearyl alcohol and behenyl alcohol. It can contain any component selected from esters such as isopropyl myristate, isopropyl palmitate, cetyl octanate, glycerin trioctate, octyldodecyl myristate, octyl stearate, stearyl stearate and the like. It can contain any component selected from phospholipids such as lecithin and its derivatives. It can contain any component selected from animal and plant-derived lipids such as bovine bone marrow fat and bovine brain lipids. It can contain any component selected from alkyl sulphates such as ammonium lauryl sulphate, ethanolamine lauryl sulphate, sodium lauryl sulphate, triethanolamine lauryl sulphate. Polyoxyethylene (2EO) lauryl ether sulphate triethanolamine (EO is ethylene oxide, the value before EO indicates the number of moles of ethylene oxide added; the same applies hereinafter), polyoxyethylene (3EO) alkyl (11 carbon atoms). Any of ~ 15 or a mixture of two or more) can contain any component selected from polyoxyethylene alkyl sulfates such as ether sodium sulfate. It can contain any component selected from alkylbenzene sulfonates such as sodium laurylbenzene sulfonate, triethanolamine laurylbenzene sulfonate. It can contain any component selected from polyoxyethylene alkyl ether sulfates such as polyoxyethylene (3EO) tridecyl ether sodium acetate. Palm oil fatty acid sarcosin sodium, lauroyl sarcosin triethanolamine, lauroylmethyl-L-sodium glutamate, coconut oil fatty acid-L-sodium glutamate, coconut oil fatty acid-L-glutamate triethanolamine, coconut oil fatty acid methyl taurine sodium, lauroylmethyl taurine N-acylamino acid salts such as sodium, sodium ether sulfate alkane sulfonate, hardened coconut oil fatty acid sodium glycerin sulfate, hardened coconut oil fatty acid sodium glycerin sulfate, undesinoylamide ethyl sulfostubate disodium, octylphenoxydientoxyethyl sulfonate sodium , Amino sulfosuccinate disodium oleate, dioctyl sulfosuccinate, dioctyl sulfosuccinate, lauryl disodium sulfosuccinate, polyoxyethylene alkyl (carbon chain 12-15) ether phosphate (8-10EO), polyoxyethylene oleyl ether Select from anionic surfactants such as sodium phosphate, sodium polyoxyethylene cetyl ether phosphate, disodium lauryl lauryl polyoxyethylene sulfosuccinate, sodium polyoxyethylene lauryl ether phosphate, sodium lauryl sulfoacetate, and sodium tetradecene sulfonate. Can contain any of the ingredients that are used. Stearyldimethylammonium chloride, di (polyoxyethylene) oleylmethylammonium, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, tri (polyoxyethylene) stearylammonium chloride, polyoxypropylenemethyldiethylammonium chloride, myristyldimethylbenzylammonium chloride , Lauryl trimethylammonium chloride can contain any component selected from cationic surfactants such as ammonium chloride. 2-alkyl-N-carboxymethyl-N-hydroxyethyl imidazolinium betaine, undecyl hydroxyethyl imidazolinium betaine sodium, undecyl-N-hydroxyethyl-N-carboxymethyl imidazolinium betaine, stearyldihydroxyethyl betaine, palm Oil fatty acid amide propyl betaine, coconut oil alkyl-N-carboxyethyl-N-hydroxyethyl imidazolinium betaine sodium, coconut oil alkyl-N-carboxymethoxyethyl-N-carboxymethyl imidazolinium disodium lauryl sulfate, N-palm It can contain any component selected from amphoteric surfactants such as the oil fatty acid acyl-L-arginine ethyl-DL-pyrrolidone carboxylate. Polyoxyethylene alkyl (12-14 carbon atoms) ether (7EO), polyoxyethylene octylphenyl ether, polyoxyethylene oleyl ether, glycerin polyoxyethylene oleate, polyoxyethylene stearyl ether, polyoxyethylene cetyl ether, polyoxy Ethylenecetylstearyldiether, polyoxyethylene sorbitol lanolin (40EO), polyoxyethylene nonylphenyl ether, polyoxyethylene polyoxypropylene cetyl ether, polyoxyethylene polyoxypropylene decyltetradecyl ether, polyoxyethylene lanolin, polyoxy It can contain any component selected from nonionic surfactants such as ethylenelanolin alcohol and polyoxypropylene stearyl ether. Isostearic acid diethanolamide, undecylenic acid monoethanolamide, oleic acid diethanolamide, beef fatty acid monoethanolamide, hardened beef fatty acid diethanolamide, stearic acid diethanolamide, stearic acid diethylaminoethylamide, stearic acid monoethanolamide, myristic acid diethanolamide, It can contain any component selected from thickeners such as coconut oil fatty acid diethanolamide, coconut oil fatty acid ethanolamide, lauric acid isopropanolamide, lauric acid ethanolamide, lauric acid diethanolamide, lanolin fatty acid diethanolamide. Optional selected from silicon oils such as chain or cyclic methylpolysiloxane, methylphenylpolysiloxane, dimethylpolysiloxane polyethylene glycol copolymer, dimethylpolysiloxanepolypolypolymer, amino-modified silicon oil, quaternary ammonium-modified silicon oil. Can contain the components of. It can contain any component selected from thioglycolic acid and salts thereof. It can contain any component selected from cysteamine and salts thereof. It can contain any component selected from peroxides such as hydrogen peroxide, persulfates, peroxides, urea peroxides and the like. It can contain any component selected from bromate such as sodium bromate and potassium bromate. It can contain any ingredient selected from other active ingredients such as keratolytic agents, astringents, wound healing agents, deodorant / deodorants, antiallergic agents, blood flow promoters, cell activators and the like.

By the way, since the external skin preparation and the skin cosmetic contain the above-mentioned culture solution, it is possible to maintain and improve the condition of the skin such as the texture improving effect and the moisturizing effect. Here, to maintain / improve the condition of the skin means to maintain and / or improve the moisturizing property of the skin, to maintain and / or improve the texture of the skin, and to prevent and / or improve the dullness of the skin. It means to include. Specifically, the texture improving effect is an effect of improving the skin to a fine texture, that is, a state in which the skin groove on the skin surface is shallow and each skin hill shows a clear, small and beautiful triangle. Further, the dullness preventing effect is to act on a skin portion called dull skin whose lightness is lower than that of the surroundings, narrow the area of the dull skin, and reduce the degree of decrease in lightness.

External skin preparations and skin cosmetics are particularly useful for administration or application to subjects having at least one symptom of dry skin, dull skin and rough skin. Such an object may be, for example, an object having a factor that tends to cause at least one of dry skin, dull skin and rough skin. Factors that are likely to cause at least one of dry skin, dull skin, and rough skin include, for example, environmental factors, lifestyle-related habits, dietary habits, genetic factors, constitution, diseases, mental / physical stress, and aging. Can be mentioned. By applying an external skin preparation and a skin cosmetic to a subject having these factors, at least one of dry skin, dull skin and rough skin can be improved, and health or cosmetic benefits can be expected.

The dosage and application amount of the external skin preparation and the skin cosmetics vary depending on the age, body weight, application / administration route, dosage form, number of administrations, etc. of the subject, and can be widely changed at the discretion of those skilled in the art. Specifically, the applied amount or dose thereof is not particularly limited, but can be, for example, 0.001 mg / time to 10 g / time in terms of the dry weight of the culture solution as the active ingredient. External skin preparations and skin cosmetics may be repeatedly administered or applied and administered at intervals of, for example, 6 to 24 hours, without limitation.

Hereinafter, the present invention will be described in more detail by way of examples, but the technical scope of the present invention is not limited to the following examples.

[Example 1] Preparation of sap yeast culture sap and control product The birch sap used in this example was all produced in Hokkaido and filtered from a stock solution that had not been concentrated or diluted.

<Manufacturing Example 1> Galactomyces reessii
A preculture medium was prepared by using birch sap from Hokkaido as a substrate for preculture and adding yeast extract and peptone as supplementary nutritional components (see Table 1). The preculture medium was placed in a flask with a baffle, covered with silicosen, and sterilized at 121 ° C. for 20 minutes. After sterilization, the preculture medium was cooled to 30 ° C. and 1 platinum loop was inoculated with Galactomyces reessii. In this Production Example 1, preculture was performed by shaking at 30 ° C. for 48 hours using a preculture medium.

At the time of main culture, birch sap (2000 g) roughly filtered using a cartridge filter with an opening of 20 μm was placed in a small jar fermenter for microbial culture and sterilized at 121 ° C. for 20 minutes. After cooling the birch sap to 30 ° C, 50 g of preculture medium was added, and galactomyces racesy was cultured at 30 ° C while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. During the culture period, sampling was appropriately performed, and the glucose concentration in the culture solution was measured by an enzymatic method (F kit glucose: JK International Co., Ltd.). The glucose content in the culture medium is shown in FIG. As shown in FIG. 1, glucose was consumed and decreased as the bacteria grew, and the glucose initially present at 1200 ppm became 20 ppm or less at the end of the culture.

The obtained culture broth was heat-treated at 121 ° C. for 30 minutes to extract bacterial cell components, and then cooled to room temperature. The obtained solution containing solids such as yeast cell wall was filtered with a pre-filter and then with a 0.45 μm membrane filter to remove solids, and a birch sap galactomyces culture solution was obtained.

<Production Example 2> Saccharomycopsis capsularis
In this Production Example 2, a birch sap Saccharomycopsis culture solution was obtained in the same manner as in Production Example 1 except that Saccharomycopsis capsularis was used. The fate of glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

<Production Example 3> Saccharomyces cerevisiae
In this Production Example 3, a birch sap Saccharomyces culture solution was obtained in the same manner as in Production Example 1 except that Saccharomyces cerevisiae was used. The fate of glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1).

<Production Example 4> Culturing with a plurality of bacteria In this Production Example 4, birch sap galactomyces was used in the same manner as in Production Example 1 except that Galactomyces reessii and Saccharomyces cerevisiae were used. -Saccharomyces culture medium was obtained. The fate of glucose during the culture period was also measured in the same manner as in Example 1 (see FIG. 1). Further, in Production Example 4, the obtained birch sap galactomyces-saccharomyces culture solution was vacuum freeze-dried to obtain a birch sap galactomyces-saccharomyces culture solution freeze-dried powder.

<Comparative Example 1> Uncultured birch sap In Comparative Example 1, birch sap (2000 g) roughly filtered using a cartridge filter with an opening of 20 μm was sterilized at 121 ° C. for 20 minutes. The sterilized birch sap was cooled to room temperature and filtered through a pre-filter and then with a 0.45 μm membrane filter to prepare a sterilized birch sap filter. Similarly, the glucose concentration of this solution was measured by an enzymatic method (F kit glucose: JK International Co., Ltd.).

<Comparative Example 2> Yeast culture with skim milk medium In Comparative Example 2, a skim milk medium was prepared as a substrate for preculture (see Table 1). The prepared preculture medium was placed in a flask with a baffle, covered with silicosen, and sterilized at 121 ° C. for 20 minutes. After sterilization, the preculture medium was cooled to 30 ° C. and 1 platinum loop was inoculated with Galactomyces reessii. In this Comparative Example 2, shaking culture was performed at 30 ° C. for 48 hours using this preculture medium.

In the main culture, 20 g of skim milk and 10 g each of glucose and yeast extract were dissolved in 1960 g of purified water, and the main culture medium was placed in a small jar fermenter for microbial culture and sterilized at 121 ° C. for 20 minutes. After cooling the main culture medium to 30 ° C., 50 g of the preculture medium was added, and galactomyces racesy was cultured at 30 ° C. for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The obtained skim milk galactomyces culture solution was heat-treated at 121 ° C. for 30 minutes to extract bacterial cell components, and then cooled to room temperature. The obtained solution containing solids such as yeast cell walls was filtered through a pre-filter and then a 0.45 μm membrane filter to obtain a skim milk galactomyces culture medium from which solids had been removed.

<Comparative Example 3> Yeast culture using soymilk medium In Comparative Example 3, soymilk medium was prepared as a substrate for preculture (see Table 1). The prepared preculture medium was placed in a flask with a baffle, covered with silicosen, and sterilized at 121 ° C. for 20 minutes. After sterilization, the preculture medium was cooled to 30 ° C. and 1 platinum loop was inoculated with Galactomyces reessii. In Comparative Example 3, the preculture medium was used for shaking culture at 30 ° C. for 48 hours.

For the main culture, 2000 g of soymilk was placed in a small jar fermenter for microbial culture and sterilized at 121 ° C for 20 minutes. After cooling the main culture medium to 30 ° C., 50 g of preculture was added, and galactomyces racesy was cultured at 30 ° C. for 48 hours while continuously adjusting the pH to 6.0 with an aqueous sodium hydroxide solution. The obtained soymilk galactomyces culture solution was heat-treated at 121 ° C. for 30 minutes to extract bacterial cell components, then cooled to room temperature, and the obtained solution containing solids such as yeast cell walls was prefiltered and subsequently. The mixture was filtered through a 0.45 μm membrane filter to obtain a soymilk galactomyces culture solution from which solid matter had been removed.

Examples 2 and below show evaluation test methods and results using the various culture solutions produced in Example 1 and the culture solutions of Comparative Examples. A list of samples used for evaluation is shown in Table 2.

[Example 2] Moisturizing evaluation based on keratin water content and water evaporation amount A lotion having the composition shown in Table 3 was prepared using the samples shown in Table 2, and the inside of the forearm of female subjects (10 persons) in their 40s and 50s. It was applied to the part twice a day for 4 consecutive weeks. The table shows the results of measuring the keratin water content before and 2 to 4 weeks after application using a Corneometer (Courage + Khazaka) and the transdermal water evaporation using a Tewameter (Courage + Khazaka electronic GmbH). 4 and Table 5 show.

As shown in Table 4, regarding the keratin water content, an increase in the water retention amount was observed in the test groups 1 to 4 and the control groups 1 to 3 as compared with the comparative example 4 using purified water. In particular, in all of Test Groups 1 to 4 in which yeast was cultivated using birch sap as a substrate component, the effect of retaining keratin water content was extremely excellent, and birch sap (control group 1) and skim milk without yeast culture were obtained. The culture solution using yeast as a substrate (control group 2) and the culture solution using soymilk as a substrate (control group 3) also showed some moisturizing effect, but the results were inferior to those of the test groups 1 to 4. In addition, when comparing the types of yeast, it was confirmed that the keratin water retention effect was high in the two test groups, the culture solution of galactomyces lasci (test group 1) and the culture solution of saccharomycopsis (test group 2). rice field.

In addition, as shown in Table 5, regarding the amount of water evaporation, an increase in the amount of water evaporation was observed in the control group 2 (purified water) due to the influence from the environment during the test period, but other test groups and controls. In the plot, the effect of suppressing transepidermal water loss was confirmed. In particular, the effect of preventing water transpiration was extremely excellent in all of the test groups 1 to 4 in which yeast was cultivated using birch sap as a substrate component. The birch sap without yeast culture (control group 1), the culture solution using skim milk as a substrate (control group 2), and the culture solution using soymilk as a substrate (control group 3) also showed some effect of preventing water evaporation. However, the result was inferior to that of the test groups 1 to 4. In addition, when comparing the types of yeast, there were two test groups, a birch sap yeast culture sap (test group 1) in which galactomyces racesi was selected and a birch sap yeast culture sap (test group 2) in which saccharomycopsis was selected. It was confirmed that the water retention and transpiration prevention effect was high.

[Example 3] Texture improvement test 1
The samples shown in Table 3 were marked on the medial forearm of female subjects (10 subjects) in their 40s and 50s in a range of 15 mm × 15 mm in each test group, and were applied twice a day for 4 consecutive weeks. The texture before and after application was photographed with a microscope (manufactured by KEYENCE CORPORATION) and compared. The images were evaluated for the following 3 items (depth of skin groove, shape of skin hill, size of skin hill) with the following 5 grades, and the average values of the scores of 10 subjects are shown in Tables 6-8. It was shown to.

(1) Deep and shallow skin groove 5 Very shallow skin groove 4 Slightly shallow skin groove 3 Normal degree of skin groove 2 Slightly deep skin groove 1 Very deep skin groove (2) Shape of skin hill 5 All are beautiful triangles 4 It is almost a triangle.
3 Skin hills other than triangles are slightly conspicuous 2 Skin hills other than triangles are conspicuous 1 Skin hills other than triangles are very conspicuous (3) Skin hill size 5 Very small and well-organized 4 Slightly small 3 Medium size Yes 2 Slightly large 1 Very large

As shown in Tables 6 to 8, the effect of improving textured skin was confirmed in Test Groups 1 to 4 and Control Groups 1 to 3 with respect to Comparative Example 4 using purified water. In particular, in all of Test Groups 1 to 4 in which yeast was cultivated using birch sap as a substrate component, the effect of improving textured skin was extremely excellent. Birch sap without yeast culture (control group 1), a culture solution using skim milk as a substrate (control group 2), and a culture solution using soymilk as a substrate (control group 3) also showed some effect of improving textured skin. However, the result was inferior to that of the test groups 1 to 4. Comparing the types of yeast, it was confirmed that the birch sap yeast culture broth (Test Group 1) in which galactomyces lasci was selected had a high effect of improving textured skin.

[Example 4] Stickiness evaluation test 1
In Example 4, in the second week of the continuous application test period in Example 2, the skin friction force evaluation device Frictiometer FR700 (Frictiometer FR700) was used for the stickiness of the application site before applying lotion and after drying for 15 minutes after application of each test group. It was measured by Courage + Khazaka) and compared. The value after application was obtained when the frictional force value before application was 100, and the average value of 10 subjects was taken and shown in Table 9.

As shown in Table 9, in the control group 1, there was stickiness due to the sugar contained, and the frictional force also showed a very high value. That is, it was clarified that the birch sap itself gives a sticky feeling. In addition, the yeast culture broths using skim milk and soymilk as substrates in the control groups 2 and 3 also showed high frictional force and stickiness, though not as much as in the control group 1. Compared with these, in the test groups 1 to 4 in which yeast was cultivated using birch sap as a substrate component, the values were close to those of the purified water in the control group 4, and the values were excellent without giving a sticky feeling. It was shown to be a feeling.

[Example 5] Texture improvement test 2
In Example 5, the samples shown in Table 10 were applied to the left and right cheeks of female subjects (10 subjects) in their 40s and 50s twice a day for 4 consecutive weeks. With the right cheek as the test plot and the left cheek as the control plot, the texture condition before and after application was measured using a skin image analyzer (VISIA TM Evolution, manufactured by Canfield), and the results of quantifying the texture evaluation were obtained. It is shown in Table 11.

As shown in Table 11, in the study in this example, the test group 1 in which the galactomyces were cultured using the birch sap as the substrate component had a higher texture improving effect than the control group using the birch sap itself. confirmed.

[Example 6] Dullness improvement test In this Example 6, the dullness improvement effect in the test group and the control group was measured in parallel with the test of Example 5. The dullness was evaluated by using a colorimeter (manufactured by Konica Minolta), which is a colorimeter, to obtain and analyze the brightness index L * value in the L * a * b * color system. The results before and after 4 consecutive weeks of application are shown in Table 12 (in the table, the L * values are the average values of the subjects (10 subjects)).

As shown in Table 12, it was confirmed that the test group 1 in which the galactomyces were cultured using the birch sap as the substrate component had a higher dullness improving effect than the control group using the birch sap itself.

[Example 7] Stickiness evaluation test 2 (sensory test)
In Example 7, a sensory test was performed on the stickiness during the tests of Examples 5 and 6. Specifically, the evaluation was carried out every week from 1 week to 4 weeks after the start of the test on the following 5 grades. Table 13 shows the results of averaging the scores of 10 subjects.
Evaluation of skin stickiness 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky

As shown in Table 13, the test plots in which galactomyces were cultured using birch sap as a substrate component gave a refreshing feel throughout the entire period, and the control plot using birch sap itself had sugar content in the sap. The result was that it gave a sticky feel due to the influence of.

[Example 8] Production example of cosmetic water In this Example 8, the components (1) to (11) are stirred at 80 ° C. and cooled to room temperature after being dissolved according to the formulation shown in Table 14, so that the cosmetic water (white birch) is cooled. A lotion containing sap yeast culture solution) was produced. Further, the control lotion was produced as in Comparative Example 1 in exactly the same manner except that the yeast-cultured birch sap prepared in Production Example 1 was not added. All of the obtained lotions were clear and stable under the conditions of 40 ° C. and 75% relative humidity (RH) for 3 months without causing cloudiness.

These lotions were subjected to a sensory test by female subjects (10 people) in their 40s and 50s. The four items (skin moistness, skin smoothness, skin stickiness, and sensory stimulation) were evaluated on the following five grades. Table 15 shows the average scores of the subjects (10 subjects).

(1) Moisturizing skin 5: Moisturizing 4: Slightly moisturizing 3: Normal 2: Slightly dry 1: Dry
(2) Skin smoothness 5: Smooth 4: Slightly smooth 3: Normal 2: Slightly rough 1: Rough
(3) Skin stickiness 5: Refreshing 4: Slightly refreshing 3: Normal 2: Slightly sticky 1: Sticky
(4) Sensory stimulation 5: No particular stimulation is felt.
4: I feel a little stimulus, but I don't mind.
3: Slight stimuli such as tingling, hotness, tingling, and swelling are acceptable.
2: Strong irritation such as tingling sensation, hot feeling, tingling sensation, and swelling.
1: Stimulation such as tingling sensation, hot feeling, tingling sensation, and swelling is very strong.
0: The stimulus is strong and the test cannot be continued.

As is shown in Table 15, as is clear from the above, the lotion containing the culture medium obtained by culturing galactomyces using birch sap as a substrate component showed better moisturizing properties than the control lotions 1 and 2. It showed the effect of suppressing stickiness.

[Example 9] Production of cream agent In this example 9, the components (1) to (7) were mixed and stirred at 80 ° C. according to the formulation shown in Table 16, and the component (8) was separately added to the mixture obtained. The mixture obtained by mixing and stirring ~ (11) at 80 ° C. was added, homogenized, and cooled to room temperature while stirring to produce a cream.

The obtained cream was not sticky during use and moisturized the skin.

[Example 10] Production of body gel agent In Example 10, components (1) to (7) were stirred and dissolved according to the formulation shown in Table 17 to produce a body gel agent. The obtained body gel agent moisturized the skin and was a non-irritating preparation.

[Example 11] Production of shampoo In Example 11, components (1) and (2) were heated and dissolved, and components (3) and (4) heated to 60 ° C. were added according to the formulation shown in Table 18. Stirred. Ingredients (5) to (8) were added thereto and stirred. The component (9) was added here so as to have a pH of 5.5 to 6.0, and further, the components (11) and (12) dissolved in the component (10) were added. Ingredient (13) was added until the total amount (100%) was reached to produce a shampoo.

When the hair was washed with the obtained shampoo, the feel of the hair became smooth and the hair could be moisturized.

[Example 12] Production of cleansing cream In Example 12, a cleansing composition was obtained by stirring and uniformly mixing all the components according to the formulation shown in Table 19. The obtained cleansing cream had good detergency and gave a refreshing after-use feeling.

[Example 13] Production of rinsing agent In Example 13, a rinsing composition was obtained by stirring and uniformly mixing all the components according to the formulation shown in Table 20. The obtained rinse gave a moist feeling after use to the hair and the scalp.

[Example 14] Production of emulsion In Example 14, an emulsion composition was obtained by stirring and uniformly mixing all the components according to the formulation shown in Table 21. The obtained milky lotion gave the skin a moist and moisturized feeling.

[Example 15] Production of mask In Example 15, a mask was prepared by impregnating a non-woven fabric with a skin external preparation having the composition shown in Table 22. The face was covered with the prepared mask for 15 minutes to give an appropriate moisturizing and tense feeling after use.

Claims (7)

A culture medium in which yeast is grown in a medium containing birch sap. The culture solution according to claim 1, wherein the birch sap is a sap obtained from Betula platyphylla. A claim characterized in that the yeast is one yeast selected from the group consisting of yeast belonging to the genus Saccharomyces, yeast belonging to the genus Saccharomyces and yeast belonging to the genus Saccharomyces, or a plurality of yeasts selected from the group. Item 3. The culture solution according to Item 1 or 2. An external skin preparation containing the culture solution according to any one of claims 1 to 3. A skin cosmetic containing the culture solution according to any one of claims 1 to 3. The skin cosmetic according to claim 5, which exhibits at least one effect selected from the group consisting of a moisturizing effect, a texture improving effect, and a dullness improving effect. During the process of culturing yeast in a medium containing birch sap, the small molecule sugar in the birch sap is consumed as a nutrient source for yeast, and the concentration of the small molecule sugar in the birch sap becomes lower than before the start of culturing. A method for producing a culture medium, which is characterized by the above.

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