JP2022046466A5 - - Google Patents
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- JP2022046466A5 JP2022046466A5 JP2021193224A JP2021193224A JP2022046466A5 JP 2022046466 A5 JP2022046466 A5 JP 2022046466A5 JP 2021193224 A JP2021193224 A JP 2021193224A JP 2021193224 A JP2021193224 A JP 2021193224A JP 2022046466 A5 JP2022046466 A5 JP 2022046466A5
- Authority
- JP
- Japan
- Prior art keywords
- nucleic acid
- adapter
- sample
- ligated
- guided nuclease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562207359P | 2015-08-19 | 2015-08-19 | |
| US62/207,359 | 2015-08-19 | ||
| JP2018508166A JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| PCT/US2016/047631 WO2017031360A1 (en) | 2015-08-19 | 2016-08-18 | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Division JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022046466A JP2022046466A (ja) | 2022-03-23 |
| JP2022046466A5 true JP2022046466A5 (https=) | 2022-06-09 |
| JP7792241B2 JP7792241B2 (ja) | 2025-12-25 |
Family
ID=58050956
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
| JP2021193224A Active JP7792241B2 (ja) | 2015-08-19 | 2021-11-29 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018508166A Active JP6986509B2 (ja) | 2015-08-19 | 2016-08-18 | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10538758B2 (https=) |
| EP (2) | EP3985111A1 (https=) |
| JP (2) | JP6986509B2 (https=) |
| CN (1) | CN108138176B (https=) |
| AU (1) | AU2016308283B2 (https=) |
| CA (1) | CA2995983A1 (https=) |
| DK (1) | DK3337898T3 (https=) |
| WO (1) | WO2017031360A1 (https=) |
Families Citing this family (62)
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|---|---|---|---|---|
| US10323236B2 (en) | 2011-07-22 | 2019-06-18 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US9163284B2 (en) | 2013-08-09 | 2015-10-20 | President And Fellows Of Harvard College | Methods for identifying a target site of a Cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9322037B2 (en) | 2013-09-06 | 2016-04-26 | President And Fellows Of Harvard College | Cas9-FokI fusion proteins and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| EP3105325B2 (en) | 2014-02-13 | 2024-10-23 | Takara Bio USA, Inc. | Methods of depleting a target molecule from an initial collection of nucleic acids, and compositions and kits for practicing the same |
| US10077453B2 (en) | 2014-07-30 | 2018-09-18 | President And Fellows Of Harvard College | CAS9 proteins including ligand-dependent inteins |
| WO2016100955A2 (en) | 2014-12-20 | 2016-06-23 | Identifygenomics, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
| US10538758B2 (en) | 2015-08-19 | 2020-01-21 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| AU2016365720B2 (en) | 2015-12-07 | 2020-11-26 | Arc Bio, Llc | Methods and compositions for the making and using of guide nucleic acids |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
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| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
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| GB201616590D0 (en) | 2016-09-29 | 2016-11-16 | Oxford Nanopore Technologies Limited | Method |
| KR102622411B1 (ko) | 2016-10-14 | 2024-01-10 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵염기 에디터의 aav 전달 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
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| US12390514B2 (en) | 2017-03-09 | 2025-08-19 | President And Fellows Of Harvard College | Cancer vaccine |
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| KR20240116572A (ko) | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| US11560566B2 (en) | 2017-05-12 | 2023-01-24 | President And Fellows Of Harvard College | Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation |
| US20200190508A1 (en) * | 2017-06-07 | 2020-06-18 | Arc Bio, Llc | Creation and use of guide nucleic acids |
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| US11142788B2 (en) | 2017-06-13 | 2021-10-12 | Genetics Research, Llc | Isolation of target nucleic acids |
| CA3069834A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Plasma/serum target enrichment |
| US10527608B2 (en) | 2017-06-13 | 2020-01-07 | Genetics Research, Llc | Methods for rare event detection |
| US10081829B1 (en) | 2017-06-13 | 2018-09-25 | Genetics Research, Llc | Detection of targeted sequence regions |
| US10947599B2 (en) | 2017-06-13 | 2021-03-16 | Genetics Research, Llc | Tumor mutation burden |
| CA3069934A1 (en) * | 2017-06-13 | 2018-12-20 | Genetics Research, Llc, D/B/A Zs Genetics, Inc. | Negative-positive enrichment for nucleic acid detection |
| KR102634727B1 (ko) * | 2017-06-14 | 2024-02-07 | 위스콘신 얼럼나이 리서어치 화운데이션 | 변형된 가이드 rna, crispr-리보뉴클레오프로테인 복합체 및 사용 방법 |
| CN107190008A (zh) * | 2017-07-19 | 2017-09-22 | 苏州吉赛基因测序科技有限公司 | 一种基于Crispr/cas9的捕获基因组目标序列的方法及其在高通量测序中的应用 |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| WO2019071048A1 (en) * | 2017-10-04 | 2019-04-11 | The Broad Institute, Inc. | SYSTEMS, METHODS AND COMPOSITIONS FOR TARGETED NUCLEIC ACID EDITION |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| CN110157727A (zh) * | 2017-12-21 | 2019-08-23 | 中国科学院遗传与发育生物学研究所 | 植物碱基编辑方法 |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| AU2019282812B2 (en) | 2018-06-07 | 2025-08-14 | Arc Bio, Llc | Compositions and methods for making guide nucleic acids |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| CN109182454A (zh) * | 2018-08-22 | 2019-01-11 | 杭州恺思医疗器械有限公司 | 一种捕获基因组特定dna片段的方法 |
| CN109837273B (zh) * | 2018-09-17 | 2021-06-29 | 东南大学 | 一种crispr辅助dna靶向富集方法及其应用 |
| EP3861135B1 (en) | 2018-10-04 | 2023-08-02 | Arc Bio, LLC | Normalization controls for managing low sample inputs in next generation sequencing |
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| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| CA3136228A1 (en) | 2019-04-09 | 2020-10-15 | Arc Bio, Llc | Compositions and methods for nucleotide modification-based depletion |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN110205318A (zh) * | 2019-05-15 | 2019-09-06 | 杭州杰毅生物技术有限公司 | 基于CRISPR-Cas去除宿主基因组DNA的宏基因组提取方法 |
| GB201913997D0 (en) * | 2019-09-27 | 2019-11-13 | Oxford Nanopore Tech Ltd | Method |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| US12173335B2 (en) * | 2020-01-06 | 2024-12-24 | Pairwise Plants Services, Inc. | Recruitment of DNA polymerase for templated editing |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| WO2021252867A2 (en) * | 2020-06-12 | 2021-12-16 | Qiagen Sciences, Llc | Methods of enriching for target nucelic acid molecules and uses thereof |
| CN112159822A (zh) * | 2020-09-30 | 2021-01-01 | 扬州大学 | 一种PS转座酶与CRISPR/dCpf1融合蛋白表达载体及其介导的定点整合方法 |
| US12522865B2 (en) * | 2020-10-06 | 2026-01-13 | Harbinger Health, Inc. | Nucleic acid enrichment method |
| CN115927539A (zh) * | 2022-09-14 | 2023-04-07 | 首都体育学院 | 靶标核酸富集方法及其试剂盒和应用 |
| WO2025132779A2 (en) * | 2023-12-22 | 2025-06-26 | F. Hoffmann-La Roche Ag | Methods and compositions for nucleic acid library and template preparation for duplexed sequencing by expansion |
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| US177444A (en) * | 1876-05-16 | Improvement in transplanters | ||
| US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
| US7166432B2 (en) | 1999-03-12 | 2007-01-23 | Integragen | Compositions and methods for genetic analysis |
| US20040209299A1 (en) * | 2003-03-07 | 2004-10-21 | Rubicon Genomics, Inc. | In vitro DNA immortalization and whole genome amplification using libraries generated from randomly fragmented DNA |
| US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
| US20090318305A1 (en) | 2008-06-18 | 2009-12-24 | Xi Erick Lin | Methods for selectively capturing and amplifying exons or targeted genomic regions from biological samples |
| WO2012051327A2 (en) | 2010-10-12 | 2012-04-19 | Cornell University | Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements |
| WO2012061832A1 (en) * | 2010-11-05 | 2012-05-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
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| US9637739B2 (en) | 2012-03-20 | 2017-05-02 | Vilnius University | RNA-directed DNA cleavage by the Cas9-crRNA complex |
| AU2013266968B2 (en) | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| US10847248B2 (en) | 2012-08-10 | 2020-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Techniques for determining haplotype by population genotype and sequence data |
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| US20140186843A1 (en) * | 2012-12-12 | 2014-07-03 | Massachusetts Institute Of Technology | Methods, systems, and apparatus for identifying target sequences for cas enzymes or crispr-cas systems for target sequences and conveying results thereof |
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| SG11201508985VA (en) | 2013-05-23 | 2015-12-30 | Univ Leland Stanford Junior | Transposition into native chromatin for personal epigenomics |
| US9873907B2 (en) * | 2013-05-29 | 2018-01-23 | Agilent Technologies, Inc. | Method for fragmenting genomic DNA using CAS9 |
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| WO2016100955A2 (en) * | 2014-12-20 | 2016-06-23 | Identifygenomics, Llc | Compositions and methods for targeted depletion, enrichment, and partitioning of nucleic acids using crispr/cas system proteins |
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| CA3001683C (en) | 2015-06-05 | 2024-06-04 | The Regents Of The University Of California | Methods and compositions for generating crispr/cas guide rnas |
| US10538758B2 (en) | 2015-08-19 | 2020-01-21 | Arc Bio, Llc | Capture of nucleic acids using a nucleic acid-guided nuclease-based system |
| US10747899B2 (en) | 2015-10-07 | 2020-08-18 | The Board Of Trustees Of The Leland Stanford Junior University | Techniques for determining whether an individual is included in ensemble genomic data |
| US20190083656A1 (en) | 2015-10-16 | 2019-03-21 | Temple University - Of The Commonwealth System Of Higher Education | Methods and compositions utilizing cpf1 for rna-guided gene editing |
| EP3978627A1 (en) | 2016-03-25 | 2022-04-06 | Karius, Inc. | Methods using synthetic nucleic acid spike-ins |
| US11046995B2 (en) | 2016-08-16 | 2021-06-29 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (FLASH) |
| US20180051320A1 (en) | 2016-08-22 | 2018-02-22 | The Regents Of The University Of California | Depletion of abundant sequences by hybridization (dash) |
| EP3526344B1 (en) | 2016-10-13 | 2020-09-30 | Biomérieux | Identification and antibiotic characterization of pathogens in metagenomic sample |
-
2016
- 2016-08-18 US US15/520,052 patent/US10538758B2/en active Active
- 2016-08-18 JP JP2018508166A patent/JP6986509B2/ja active Active
- 2016-08-18 CN CN201680057464.3A patent/CN108138176B/zh active Active
- 2016-08-18 EP EP21188035.6A patent/EP3985111A1/en active Pending
- 2016-08-18 EP EP16837863.6A patent/EP3337898B1/en active Active
- 2016-08-18 WO PCT/US2016/047631 patent/WO2017031360A1/en not_active Ceased
- 2016-08-18 CA CA2995983A patent/CA2995983A1/en active Pending
- 2016-08-18 AU AU2016308283A patent/AU2016308283B2/en active Active
- 2016-08-18 DK DK16837863.6T patent/DK3337898T3/da active
-
2019
- 2019-12-02 US US16/700,493 patent/US20200087652A1/en not_active Abandoned
-
2021
- 2021-11-29 JP JP2021193224A patent/JP7792241B2/ja active Active
-
2023
- 2023-02-01 US US18/163,196 patent/US20240132872A1/en active Pending
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