DK2992114T3 - Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA - Google Patents
Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA Download PDFInfo
- Publication number
- DK2992114T3 DK2992114T3 DK14794007.6T DK14794007T DK2992114T3 DK 2992114 T3 DK2992114 T3 DK 2992114T3 DK 14794007 T DK14794007 T DK 14794007T DK 2992114 T3 DK2992114 T3 DK 2992114T3
- Authority
- DK
- Denmark
- Prior art keywords
- enrichment
- small amounts
- samples containing
- dna
- containing small
- Prior art date
Links
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00585—Parallel processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00583—Features relative to the processes being carried out
- B01J2219/00596—Solid-phase processes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
- B01J2219/00718—Type of compounds synthesised
- B01J2219/0072—Organic compounds
- B01J2219/00722—Nucleotides
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201361819564P | 2013-05-04 | 2013-05-04 | |
PCT/US2014/036670 WO2014182574A1 (en) | 2013-05-04 | 2014-05-02 | Enrichment of dna sequencing libraries from samples containing small amounts of target dna |
Publications (1)
Publication Number | Publication Date |
---|---|
DK2992114T3 true DK2992114T3 (da) | 2019-06-24 |
Family
ID=51867654
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK14794007.6T DK2992114T3 (da) | 2013-05-04 | 2014-05-02 | Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA |
Country Status (5)
Country | Link |
---|---|
US (2) | US10576446B2 (da) |
EP (1) | EP2992114B1 (da) |
CN (1) | CN105358714B (da) |
DK (1) | DK2992114T3 (da) |
WO (1) | WO2014182574A1 (da) |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9670485B2 (en) | 2014-02-15 | 2017-06-06 | The Board Of Trustees Of The Leland Stanford Junior University | Partitioning of DNA sequencing libraries into host and microbial components |
DK3234200T3 (da) | 2014-12-20 | 2021-08-23 | Arc Bio Llc | Fremgangsmåde til målrettet depletering af nukleinsyrer ved anvendelse af CRISPR/CAS-systemproteiner |
CN105332063B (zh) * | 2015-08-13 | 2017-04-12 | 厦门飞朔生物技术有限公司 | 一种单管高通量测序文库的构建方法 |
JP6986509B2 (ja) | 2015-08-19 | 2021-12-22 | アーク バイオ, エルエルシー | 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉 |
CN105844116B (zh) * | 2016-03-18 | 2018-02-27 | 广州市锐博生物科技有限公司 | 测序数据的处理方法和处理装置 |
CN110248724B (zh) * | 2016-09-21 | 2022-11-18 | 特韦斯特生物科学公司 | 基于核酸的数据存储 |
US11999948B2 (en) * | 2017-05-04 | 2024-06-04 | The Regents Of The University Of California | Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same |
CN109402241A (zh) * | 2017-08-07 | 2019-03-01 | 深圳华大基因研究院 | 鉴定和分析古dna样本的方法 |
WO2019217486A1 (en) * | 2018-05-08 | 2019-11-14 | Memorial Sloan Kettering Cancer Center | Methods and compositions for detecting myeloma |
US20210292746A1 (en) * | 2018-08-06 | 2021-09-23 | Northwestern University | Enhanced capture of target nucleic acids |
CN111455021B (zh) * | 2019-01-18 | 2024-06-04 | 广州微远医疗器械有限公司 | 去除宏基因组中宿主dna的方法及试剂盒 |
US20220366313A1 (en) * | 2019-02-14 | 2022-11-17 | University Of Washington | Systems and methods for improved nanopore-based analysis of nucleic acids |
CN111518879B (zh) * | 2020-04-24 | 2021-02-12 | 上海茂槿生物技术有限公司 | 一种提高多重pcr扩增文库质量的方法 |
CN113774052A (zh) * | 2021-09-30 | 2021-12-10 | 首都医科大学附属北京安贞医院 | mtDNA的提取和建库试剂 |
CN114395554B (zh) * | 2022-02-18 | 2023-03-24 | 中国林业科学研究院木材工业研究所 | 一种木质文物的古dna捕获方法 |
Family Cites Families (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH08505531A (ja) * | 1993-01-15 | 1996-06-18 | ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク | Rnaバイナリ・プローブとリボザイムリガーゼを用いたrna検定法 |
US5766891A (en) | 1994-12-19 | 1998-06-16 | Sloan-Kettering Institute For Cancer Research | Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase |
US5795782A (en) | 1995-03-17 | 1998-08-18 | President & Fellows Of Harvard College | Characterization of individual polymer molecules based on monomer-interface interactions |
WO2000062036A1 (en) * | 1999-04-12 | 2000-10-19 | Nanogen/Becton Dickinson Partnership | Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology |
US7270969B2 (en) * | 1999-05-05 | 2007-09-18 | Phylogica Limited | Methods of constructing and screening diverse expression libraries |
JP2003502166A (ja) | 1999-06-22 | 2003-01-21 | プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ | 固体状態の次元的特徴の制御 |
US7258838B2 (en) | 1999-06-22 | 2007-08-21 | President And Fellows Of Harvard College | Solid state molecular probe device |
US6391592B1 (en) * | 2000-12-14 | 2002-05-21 | Affymetrix, Inc. | Blocker-aided target amplification of nucleic acids |
WO2006028508A2 (en) | 2004-03-23 | 2006-03-16 | President And Fellows Of Harvard College | Methods and apparatus for characterizing polynucleotides |
AU2005272823B2 (en) | 2004-08-13 | 2012-04-12 | President And Fellows Of Harvard College | An ultra high-throughput opti-nanopore DNA readout platform |
CA2673017C (en) * | 2006-12-21 | 2015-08-04 | Gen-Probe Incorporated | Methods and compositions for nucleic acid amplification |
EP2245198A1 (en) * | 2008-02-04 | 2010-11-03 | Massachusetts Institute of Technology | Selection of nucleic acids by solution hybridization to oligonucleotide baits |
US8497065B2 (en) | 2008-02-15 | 2013-07-30 | Life Technologies Corporation | Methods and kits for extraction of DNA |
US8029993B2 (en) * | 2008-04-30 | 2011-10-04 | Population Genetics Technologies Ltd. | Asymmetric adapter library construction |
US8883421B2 (en) * | 2010-09-10 | 2014-11-11 | New England Biolabs, Inc. | Method for reducing adapter-dimer formation |
US8575071B2 (en) * | 2010-11-03 | 2013-11-05 | Illumina, Inc. | Reducing adapter dimer formation |
US20130230857A1 (en) * | 2010-11-05 | 2013-09-05 | The Broad Institute, Inc. | Hybrid selection using genome-wide baits for selective genome enrichment in mixed samples |
US8980553B2 (en) | 2011-04-02 | 2015-03-17 | New England Biolabs, Inc. | Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides |
CN104271770A (zh) | 2012-04-30 | 2015-01-07 | 奇亚根有限公司 | 靶dna富集和测序 |
US9670485B2 (en) | 2014-02-15 | 2017-06-06 | The Board Of Trustees Of The Leland Stanford Junior University | Partitioning of DNA sequencing libraries into host and microbial components |
US9469972B2 (en) | 2014-07-07 | 2016-10-18 | Caterpillar Inc. | Adaptive control system for cyclic excavation machine |
-
2014
- 2014-05-02 CN CN201480038300.7A patent/CN105358714B/zh active Active
- 2014-05-02 US US14/653,748 patent/US10576446B2/en active Active
- 2014-05-02 EP EP14794007.6A patent/EP2992114B1/en active Active
- 2014-05-02 DK DK14794007.6T patent/DK2992114T3/da active
- 2014-05-02 WO PCT/US2014/036670 patent/WO2014182574A1/en active Application Filing
-
2020
- 2020-01-15 US US16/743,501 patent/US10981137B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
US10576446B2 (en) | 2020-03-03 |
CN105358714A (zh) | 2016-02-24 |
EP2992114B1 (en) | 2019-04-17 |
WO2014182574A1 (en) | 2014-11-13 |
US20150360194A1 (en) | 2015-12-17 |
EP2992114A4 (en) | 2017-01-04 |
US20200139335A1 (en) | 2020-05-07 |
US10981137B2 (en) | 2021-04-20 |
EP2992114A1 (en) | 2016-03-09 |
CN105358714B (zh) | 2020-03-24 |
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