DK2992114T3 - Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA - Google Patents

Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA Download PDF

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Publication number
DK2992114T3
DK2992114T3 DK14794007.6T DK14794007T DK2992114T3 DK 2992114 T3 DK2992114 T3 DK 2992114T3 DK 14794007 T DK14794007 T DK 14794007T DK 2992114 T3 DK2992114 T3 DK 2992114T3
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Denmark
Prior art keywords
enrichment
small amounts
samples containing
dna
containing small
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DK14794007.6T
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English (en)
Inventor
Meredith L Carpenter
Jason D Buenrostro
William J Greenleaf
Carlos D Bustamante
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Univ Leland Stanford Junior
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809Methods for determination or identification of nucleic acids involving differential detection
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00585Parallel processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583Features relative to the processes being carried out
    • B01J2219/00596Solid-phase processes
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718Type of compounds synthesised
    • B01J2219/0072Organic compounds
    • B01J2219/00722Nucleotides

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK14794007.6T 2013-05-04 2014-05-02 Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA DK2992114T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361819564P 2013-05-04 2013-05-04
PCT/US2014/036670 WO2014182574A1 (en) 2013-05-04 2014-05-02 Enrichment of dna sequencing libraries from samples containing small amounts of target dna

Publications (1)

Publication Number Publication Date
DK2992114T3 true DK2992114T3 (da) 2019-06-24

Family

ID=51867654

Family Applications (1)

Application Number Title Priority Date Filing Date
DK14794007.6T DK2992114T3 (da) 2013-05-04 2014-05-02 Berigelse af DNA-sekvensering biblioteker fra prøver indeholdende små mængder af mål-DNA

Country Status (5)

Country Link
US (2) US10576446B2 (da)
EP (1) EP2992114B1 (da)
CN (1) CN105358714B (da)
DK (1) DK2992114T3 (da)
WO (1) WO2014182574A1 (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9670485B2 (en) 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
DK3234200T3 (da) 2014-12-20 2021-08-23 Arc Bio Llc Fremgangsmåde til målrettet depletering af nukleinsyrer ved anvendelse af CRISPR/CAS-systemproteiner
CN105332063B (zh) * 2015-08-13 2017-04-12 厦门飞朔生物技术有限公司 一种单管高通量测序文库的构建方法
JP6986509B2 (ja) 2015-08-19 2021-12-22 アーク バイオ, エルエルシー 核酸ガイド化ヌクレアーゼをベースとした系を使用する核酸の捕捉
CN105844116B (zh) * 2016-03-18 2018-02-27 广州市锐博生物科技有限公司 测序数据的处理方法和处理装置
CN110248724B (zh) * 2016-09-21 2022-11-18 特韦斯特生物科学公司 基于核酸的数据存储
US11999948B2 (en) * 2017-05-04 2024-06-04 The Regents Of The University Of California Methods of producing size-selected nucleic acid libraries and compositions and kits for practicing same
CN109402241A (zh) * 2017-08-07 2019-03-01 深圳华大基因研究院 鉴定和分析古dna样本的方法
WO2019217486A1 (en) * 2018-05-08 2019-11-14 Memorial Sloan Kettering Cancer Center Methods and compositions for detecting myeloma
US20210292746A1 (en) * 2018-08-06 2021-09-23 Northwestern University Enhanced capture of target nucleic acids
CN111455021B (zh) * 2019-01-18 2024-06-04 广州微远医疗器械有限公司 去除宏基因组中宿主dna的方法及试剂盒
US20220366313A1 (en) * 2019-02-14 2022-11-17 University Of Washington Systems and methods for improved nanopore-based analysis of nucleic acids
CN111518879B (zh) * 2020-04-24 2021-02-12 上海茂槿生物技术有限公司 一种提高多重pcr扩增文库质量的方法
CN113774052A (zh) * 2021-09-30 2021-12-10 首都医科大学附属北京安贞医院 mtDNA的提取和建库试剂
CN114395554B (zh) * 2022-02-18 2023-03-24 中国林业科学研究院木材工业研究所 一种木质文物的古dna捕获方法

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH08505531A (ja) * 1993-01-15 1996-06-18 ザ パブリック ヘルス リサーチ インスティチュート オブ ザ シティー オブ ニューヨーク インク Rnaバイナリ・プローブとリボザイムリガーゼを用いたrna検定法
US5766891A (en) 1994-12-19 1998-06-16 Sloan-Kettering Institute For Cancer Research Method for molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
WO2000062036A1 (en) * 1999-04-12 2000-10-19 Nanogen/Becton Dickinson Partnership Amplification and separation of nucleic acid sequences using strand displacement amplification and bioelectronic microchip technology
US7270969B2 (en) * 1999-05-05 2007-09-18 Phylogica Limited Methods of constructing and screening diverse expression libraries
JP2003502166A (ja) 1999-06-22 2003-01-21 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 固体状態の次元的特徴の制御
US7258838B2 (en) 1999-06-22 2007-08-21 President And Fellows Of Harvard College Solid state molecular probe device
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
AU2005272823B2 (en) 2004-08-13 2012-04-12 President And Fellows Of Harvard College An ultra high-throughput opti-nanopore DNA readout platform
CA2673017C (en) * 2006-12-21 2015-08-04 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
EP2245198A1 (en) * 2008-02-04 2010-11-03 Massachusetts Institute of Technology Selection of nucleic acids by solution hybridization to oligonucleotide baits
US8497065B2 (en) 2008-02-15 2013-07-30 Life Technologies Corporation Methods and kits for extraction of DNA
US8029993B2 (en) * 2008-04-30 2011-10-04 Population Genetics Technologies Ltd. Asymmetric adapter library construction
US8883421B2 (en) * 2010-09-10 2014-11-11 New England Biolabs, Inc. Method for reducing adapter-dimer formation
US8575071B2 (en) * 2010-11-03 2013-11-05 Illumina, Inc. Reducing adapter dimer formation
US20130230857A1 (en) * 2010-11-05 2013-09-05 The Broad Institute, Inc. Hybrid selection using genome-wide baits for selective genome enrichment in mixed samples
US8980553B2 (en) 2011-04-02 2015-03-17 New England Biolabs, Inc. Methods and compositions for enriching either target polynucleotides or non-target polynucleotides from a mixture of target and non-target polynucleotides
CN104271770A (zh) 2012-04-30 2015-01-07 奇亚根有限公司 靶dna富集和测序
US9670485B2 (en) 2014-02-15 2017-06-06 The Board Of Trustees Of The Leland Stanford Junior University Partitioning of DNA sequencing libraries into host and microbial components
US9469972B2 (en) 2014-07-07 2016-10-18 Caterpillar Inc. Adaptive control system for cyclic excavation machine

Also Published As

Publication number Publication date
US10576446B2 (en) 2020-03-03
CN105358714A (zh) 2016-02-24
EP2992114B1 (en) 2019-04-17
WO2014182574A1 (en) 2014-11-13
US20150360194A1 (en) 2015-12-17
EP2992114A4 (en) 2017-01-04
US20200139335A1 (en) 2020-05-07
US10981137B2 (en) 2021-04-20
EP2992114A1 (en) 2016-03-09
CN105358714B (zh) 2020-03-24

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