JP2022037655A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2022037655A5 JP2022037655A5 JP2020141895A JP2020141895A JP2022037655A5 JP 2022037655 A5 JP2022037655 A5 JP 2022037655A5 JP 2020141895 A JP2020141895 A JP 2020141895A JP 2020141895 A JP2020141895 A JP 2020141895A JP 2022037655 A5 JP2022037655 A5 JP 2022037655A5
- Authority
- JP
- Japan
- Prior art keywords
- sorting
- cells
- dispensing
- reservoir
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 description 50
- 239000007788 liquid Substances 0.000 description 17
- 238000000034 method Methods 0.000 description 17
- 239000000839 emulsion Substances 0.000 description 16
- 239000002245 particle Substances 0.000 description 16
- 239000003921 oil Substances 0.000 description 15
- 239000000523 sample Substances 0.000 description 11
- 210000000265 leukocyte Anatomy 0.000 description 10
- 239000002480 mineral oil Substances 0.000 description 10
- 235000010446 mineral oil Nutrition 0.000 description 10
- 238000011084 recovery Methods 0.000 description 10
- YCKRFDGAMUMZLT-UHFFFAOYSA-N Fluorine atom Chemical compound [F] YCKRFDGAMUMZLT-UHFFFAOYSA-N 0.000 description 9
- 239000011737 fluorine Substances 0.000 description 9
- 229910052731 fluorine Inorganic materials 0.000 description 9
- 238000010586 diagram Methods 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 4
- 238000000746 purification Methods 0.000 description 4
- 102000011782 Keratins Human genes 0.000 description 3
- 108010076876 Keratins Proteins 0.000 description 3
- 230000003252 repetitive effect Effects 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000001000 micrograph Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 208000005443 Circulating Neoplastic Cells Diseases 0.000 description 1
- 238000011166 aliquoting Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 210000005266 circulating tumour cell Anatomy 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000019000 fluorine Nutrition 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 210000003714 granulocyte Anatomy 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012758 nuclear staining Methods 0.000 description 1
- RVZRBWKZFJCCIB-UHFFFAOYSA-N perfluorotributylamine Chemical compound FC(F)(F)C(F)(F)C(F)(F)C(F)(F)N(C(F)(F)C(F)(F)C(F)(F)C(F)(F)F)C(F)(F)C(F)(F)C(F)(F)C(F)(F)F RVZRBWKZFJCCIB-UHFFFAOYSA-N 0.000 description 1
- 239000000088 plastic resin Substances 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000012488 sample solution Substances 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000010729 system oil Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Priority Applications (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020141895A JP7440905B2 (ja) | 2020-08-25 | 2020-08-25 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
| US17/445,789 US11826752B2 (en) | 2020-08-25 | 2021-08-24 | Method for purifying particles, method for dispensing a single particle, method for analyzing cell cluster, and apparatus using the same |
| EP21192723.1A EP3960293A3 (en) | 2020-08-25 | 2021-08-24 | Method for purifying particles, method for dispensing a single particle, method for analyzing cell cluster, and apparatus using the same |
| CN202110981897.0A CN114112869A (zh) | 2020-08-25 | 2021-08-25 | 颗粒纯化方法、单一颗粒分注方法及细胞簇分析方法、以及其使用装置 |
| US18/521,841 US12453969B2 (en) | 2020-08-25 | 2023-11-28 | Method for purifying particles, method for dispensing a single particle, method for analyzing cell cluster, and apparatus using the same |
| JP2024017971A JP7698347B2 (ja) | 2020-08-25 | 2024-02-08 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
| JP2025094547A JP2025116267A (ja) | 2020-08-25 | 2025-06-06 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020141895A JP7440905B2 (ja) | 2020-08-25 | 2020-08-25 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024017971A Division JP7698347B2 (ja) | 2020-08-25 | 2024-02-08 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2022037655A JP2022037655A (ja) | 2022-03-09 |
| JP2022037655A5 true JP2022037655A5 (enExample) | 2022-09-08 |
| JP7440905B2 JP7440905B2 (ja) | 2024-02-29 |
Family
ID=77465850
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020141895A Active JP7440905B2 (ja) | 2020-08-25 | 2020-08-25 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
| JP2024017971A Active JP7698347B2 (ja) | 2020-08-25 | 2024-02-08 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
| JP2025094547A Pending JP2025116267A (ja) | 2020-08-25 | 2025-06-06 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024017971A Active JP7698347B2 (ja) | 2020-08-25 | 2024-02-08 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
| JP2025094547A Pending JP2025116267A (ja) | 2020-08-25 | 2025-06-06 | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 |
Country Status (4)
| Country | Link |
|---|---|
| US (2) | US11826752B2 (enExample) |
| EP (1) | EP3960293A3 (enExample) |
| JP (3) | JP7440905B2 (enExample) |
| CN (1) | CN114112869A (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115369004A (zh) * | 2022-08-24 | 2022-11-22 | 长春长光辰英生物科学仪器有限公司 | 一种用于光镊精准分选微颗粒并具备油包水结构的芯片 |
| CN116008157B (zh) * | 2023-03-28 | 2023-06-06 | 赛雷纳（中国）医疗科技有限公司 | 光路设备及流式细胞仪 |
| JP7760736B2 (ja) * | 2023-06-13 | 2025-10-27 | 広州凱普医薬科技有限公司 | 生細胞を選別するためのマイクロ流体チップ |
| WO2025033338A1 (ja) * | 2023-08-04 | 2025-02-13 | 株式会社島津製作所 | 細胞培養装置および細胞培養方法 |
| GB202408457D0 (en) * | 2024-06-13 | 2024-07-31 | Univ Oxford Innovation Ltd | Method and device for generating a metric representing a property of red blood cells in a sample |
Family Cites Families (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE60221036T2 (de) | 2001-02-23 | 2007-10-11 | Japan Science And Technology Agency, Kawaguchi | Vorrichtung zum Erzeugen von Emulsionen und Mikrokapseln |
| US6976590B2 (en) | 2002-06-24 | 2005-12-20 | Cytonome, Inc. | Method and apparatus for sorting particles |
| US7803523B2 (en) | 2004-08-27 | 2010-09-28 | University Health Network | Whole blood preparation for cytometric analysis of cell signaling pathways |
| WO2006103920A1 (ja) * | 2005-03-29 | 2006-10-05 | Sysmex Corporation | 癌・異型細胞および凝集粒子を弁別する方法および細胞分析装置 |
| US8029744B2 (en) * | 2005-11-16 | 2011-10-04 | Hitachi, Ltd. | Method of liquid droplet formation and transport apparatus therefor and particle manipulating apparatus |
| JP2007330201A (ja) | 2006-06-16 | 2007-12-27 | Ab Size:Kk | 細胞分取用マイクロチップ及び細胞分取方法 |
| CA2738578C (en) * | 2008-09-23 | 2021-05-04 | Quantalife, Inc. | Droplet-based assay system |
| RU2490050C2 (ru) * | 2009-04-07 | 2013-08-20 | Твистер Б.В. | Разделительная система, содержащая вихревой клапан |
| CN102171551B (zh) | 2009-12-25 | 2013-08-21 | 古河电气工业株式会社 | 检体识别分取装置及检体识别分取方法 |
| US10101261B2 (en) * | 2010-01-15 | 2018-10-16 | On-Chip Biotechnologies Co., Ltd. | Disposable chip-type flow cell and cell sorter using the same |
| EP2550528B1 (en) | 2010-03-25 | 2019-09-11 | Bio-Rad Laboratories, Inc. | Droplet generation for droplet-based assays |
| WO2011154042A1 (en) | 2010-06-10 | 2011-12-15 | Albert-Ludwigs-Universität Freiburg | Apparatus and method for dispensing cells or particles confined in a free flying droplet |
| JP5943521B2 (ja) | 2010-11-19 | 2016-07-05 | 株式会社オンチップ・バイオテクノロジーズ | 高濃度夾雑細胞群から低濃度の特定細胞を検出する方法と検出した細胞を回収し解析する方法 |
| US8993311B2 (en) | 2011-01-21 | 2015-03-31 | Innovative Micro Technology | Multi-stage cartridge for MEMS particle storing system |
| CN102251013A (zh) | 2011-02-22 | 2011-11-23 | 北京市肿瘤防治研究所 | 一个识别肿瘤起始细胞的抗体和抗原及其应用 |
| JP2015050965A (ja) * | 2013-09-06 | 2015-03-19 | セイコーエプソン株式会社 | 核酸増幅反応用カートリッジ |
| WO2016078339A1 (zh) * | 2014-11-17 | 2016-05-26 | 中国科学院微生物研究所 | 微液滴生成装置、系统、方法及单细胞/单分子分析装置 |
| WO2016182034A1 (ja) | 2015-05-12 | 2016-11-17 | 株式会社オンチップ・バイオテクノロジーズ | 単一粒子解析方法およびその解析のためのシステム |
| WO2018008083A1 (ja) * | 2016-07-05 | 2018-01-11 | 株式会社日立製作所 | Ｄｎａ検出方法およびそのための装置 |
| JP7020689B2 (ja) * | 2016-09-16 | 2022-02-16 | 株式会社オンチップ・バイオテクノロジーズ | 微粒子分注装置、微粒子解析装置、及び反応検出装置、並びにそれらを用いる方法 |
| JP6234542B1 (ja) * | 2016-12-27 | 2017-11-22 | 株式会社 TL Genomics | 胎児細胞由来染色体ｄｎａの取得方法 |
| GB2566002B (en) | 2017-06-22 | 2019-10-23 | Sphere Fluidics Ltd | Droplet dispensing systems |
| CN109772480B (zh) * | 2017-11-15 | 2020-11-10 | 中国科学院青岛生物能源与过程研究所 | 单个微粒包裹液滴在微流控芯片中形成并分别导出的方法 |
| JP7338336B2 (ja) | 2018-09-10 | 2023-09-05 | ソニーグループ株式会社 | 微小粒子分取装置、細胞治療薬製造装置、微小粒子分取方法、及びプログラム |
| WO2020054735A1 (en) * | 2018-09-10 | 2020-03-19 | Sony Corporation | Microparticle sorting device, cell therapeutic agent manufacturing device, microparticle sorting method and program |
| CN110793905A (zh) * | 2019-11-06 | 2020-02-14 | 中国科学院苏州生物医学工程技术研究所 | 一种微流体芯片及微流体细胞分选系统 |
| JP7458872B2 (ja) * | 2020-04-13 | 2024-04-01 | 株式会社日立ハイテク | 液滴搬送デバイス、分析システム及び分析方法 |
-
2020
- 2020-08-25 JP JP2020141895A patent/JP7440905B2/ja active Active
-
2021
- 2021-08-24 US US17/445,789 patent/US11826752B2/en active Active
- 2021-08-24 EP EP21192723.1A patent/EP3960293A3/en active Pending
- 2021-08-25 CN CN202110981897.0A patent/CN114112869A/zh active Pending
-
2023
- 2023-11-28 US US18/521,841 patent/US12453969B2/en active Active
-
2024
- 2024-02-08 JP JP2024017971A patent/JP7698347B2/ja active Active
-
2025
- 2025-06-06 JP JP2025094547A patent/JP2025116267A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2022037655A5 (enExample) | ||
| JP7698347B2 (ja) | 粒子の純化方法、単一粒子分注方法、及び細胞クラスター解析方法、並びそれに用いる装置 | |
| JP7283798B2 (ja) | 単一粒子解析方法およびその解析のためのシステム | |
| US12188858B2 (en) | Microfluidic system with combined electrical and optical detection for high accuracy particle sorting and methods thereof | |
| US20100120077A1 (en) | Microfluidic particle-analysis systems | |
| KR20180015238A (ko) | 시스템들 및 방법들 | |
| JP2020508468A5 (enExample) | ||
| JP7734312B2 (ja) | 粒子を分類するためのシステムおよび方法 | |
| US12000852B2 (en) | Apparatus and method for isolating single particles from a particle suspension | |
| Inglis et al. | A scalable approach for high throughput branch flow filtration | |
| JP7287399B2 (ja) | 微小粒子分取用流路ユニット及び微小粒子分取装置 | |
| US20190025183A1 (en) | Gene analysis method | |
| WO2025205054A1 (ja) | 解析システム、解析方法、およびプログラム | |
| WO2025244564A1 (en) | System and processes for detecting, classifying and dispensing particles | |
| WO2025255586A1 (en) | Integrated droplet sorter module for microfluidic devices | |
| HK1129211A (en) | Apparatus for continuous particle separation |