JP2021525105A - 標的細菌を死滅させるための方法および組成物 - Google Patents

標的細菌を死滅させるための方法および組成物 Download PDF

Info

Publication number
JP2021525105A
JP2021525105A JP2021516552A JP2021516552A JP2021525105A JP 2021525105 A JP2021525105 A JP 2021525105A JP 2021516552 A JP2021516552 A JP 2021516552A JP 2021516552 A JP2021516552 A JP 2021516552A JP 2021525105 A JP2021525105 A JP 2021525105A
Authority
JP
Japan
Prior art keywords
bacteriophage
crispr
target
gene
essential
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2021516552A
Other languages
English (en)
Japanese (ja)
Other versions
JP2021525105A5 (enExample
Inventor
エム． ガロフォロ，ポール
エム． ガロフォロ，ポール
ジー． オースターアウト，デビッド
ジー． オースターアウト，デビッド
セル，カート
ウォン，サンディ
ローレンス ベイセル，チェイス
ローレンス ベイセル，チェイス
クマー シン，アチュル
クマー シン，アチュル
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
North Carolina State University
Original Assignee
North Carolina State University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by North Carolina State University filed Critical North Carolina State University
Publication of JP2021525105A publication Critical patent/JP2021525105A/ja
Publication of JP2021525105A5 publication Critical patent/JP2021525105A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/40—Viruses, e.g. bacteriophages
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/76—Viruses; Subviral particles; Bacteriophages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2795/00—Bacteriophages
    • C12N2795/00011—Details
    • C12N2795/14011—Details ssDNA Bacteriophages
    • C12N2795/14033—Use of viral protein as therapeutic agent other than vaccine, e.g. apoptosis inducing or anti-inflammatory
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dentistry (AREA)
  • Agronomy & Crop Science (AREA)
  • Immunology (AREA)
  • Environmental Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicinal Preparation (AREA)
JP2021516552A 2018-05-25 2019-05-24 標的細菌を死滅させるための方法および組成物 Pending JP2021525105A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862676818P 2018-05-25 2018-05-25
US62/676,818 2018-05-25
PCT/US2019/034061 WO2019227080A1 (en) 2018-05-25 2019-05-24 Methods and compositions for killing a target bacterium

Publications (2)

Publication Number Publication Date
JP2021525105A true JP2021525105A (ja) 2021-09-24
JP2021525105A5 JP2021525105A5 (enExample) 2022-05-26

Family

ID=68616480

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2021516552A Pending JP2021525105A (ja) 2018-05-25 2019-05-24 標的細菌を死滅させるための方法および組成物

Country Status (4)

Country Link
US (1) US20210161150A1 (enExample)
EP (1) EP3817556A4 (enExample)
JP (1) JP2021525105A (enExample)
WO (1) WO2019227080A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854758A (zh) * 2022-06-14 2022-08-05 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB202007943D0 (en) 2020-05-27 2020-07-08 Snipr Biome Aps Products & methods
US20240011041A1 (en) * 2020-11-05 2024-01-11 Locus Biosciences, Inc. Phage compositions for pseudomonas comprising crispr-cas systems and methods of use thereof
WO2022098899A1 (en) * 2020-11-05 2022-05-12 Locus Biosciences, Inc. Phage compositions for escherichia comprising crispr-cas systems and methods of use thereof
CN117729853A (zh) * 2020-11-05 2024-03-19 洛可斯生物科学公司 包含crispr-cas系统的用于假单胞菌的噬菌体组合物及其使用方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto
US20170106025A1 (en) * 2011-02-04 2017-04-20 Joseph E. Kovarik Method and System for Treating Gastro-Esophageal Reflux Disease (GERD)
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8193314B2 (en) * 2007-07-12 2012-06-05 Massachusetts Institute Of Technology Transcriptional engineering of Lactobacillus
EP4074330A1 (en) * 2013-09-05 2022-10-19 Massachusetts Institute of Technology Tuning microbial populations with programmable nucleases
WO2016177682A1 (en) * 2015-05-06 2016-11-10 Snipr Technologies Limited Altering microbial populations & modifying microbiota
EP3426784A1 (en) * 2016-03-11 2019-01-16 Wageningen Universiteit Improved crispr-cpf1 genome editing tool
EP3429635B1 (en) * 2016-03-15 2026-05-06 University of Massachusetts Anti-crispr compounds and methods of use
US11845929B2 (en) * 2016-07-08 2023-12-19 Ohio State Innovation Foundation Modified nucleic acids, hybrid guide RNAs, and uses thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170106025A1 (en) * 2011-02-04 2017-04-20 Joseph E. Kovarik Method and System for Treating Gastro-Esophageal Reflux Disease (GERD)
JP2017513489A (ja) * 2014-04-14 2017-06-01 ネメシス バイオサイエンス リミティド 治療薬
WO2017015015A1 (en) * 2015-07-17 2017-01-26 Emory University Crispr-associated protein from francisella and uses related thereto

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
SCIENTIFIC REPORTS, vol. 7, no. 44929, JPN6023022804, 2017, pages 1 - 13, ISSN: 0005075162 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114854758A (zh) * 2022-06-14 2022-08-05 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用
CN114854758B (zh) * 2022-06-14 2023-09-12 四川大学 基于CRISPR-Cas13a系统靶向杀死沙门氏菌的方法及其应用

Also Published As

Publication number Publication date
EP3817556A1 (en) 2021-05-12
US20210161150A1 (en) 2021-06-03
EP3817556A4 (en) 2022-03-30
WO2019227080A1 (en) 2019-11-28

Similar Documents

Publication Publication Date Title
US20220387531A1 (en) Methods and compositions for killing a target bacterium
EP4055152A1 (en) Phage compositions comprising crispr-cas systems and methods of use thereof
US20210161150A1 (en) Methods and compositions for killing a target bacterium
TW202233832A (zh) 針對假單胞菌屬之包含ｃｒｉｓｐｒ－ｃａｓ系統的噬菌體組合物及其使用方法
US20220411782A1 (en) Phage compositions comprising crispr-cas systems and methods of use thereof
US20220389392A1 (en) Crispr cas systems and lysogeny modules
US20250345378A1 (en) Phage compositions for escherichia comprising crispr-cas systems and methods of use thereof
US20240067935A1 (en) Altering the normal balance of microbial populations
WO2022235816A2 (en) Bacteriophage comprising type i crispr-cas systems
US20230398161A1 (en) Phage compositions for escherichia comprising crispr-cas systems and methods of use thereof
TW202305126A (zh) 針對假單胞菌屬之包含crispr-cas系統的噬菌體組合物及其使用方法
US20260124259A1 (en) Staphylococcus phage compositions and cocktails thereof
CN117651764A (zh) 包含crispr-cas系统的用于埃希氏菌的噬菌体组合物及其使用方法

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210312

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210819

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20210819

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20220518

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20220519

RD12 Notification of acceptance of power of sub attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7432

Effective date: 20230303

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A821

Effective date: 20230303

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20230605

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20240105