JP2021504690A - 連続流動サンプリングプローブを使用する分析器具の音響装填のためのシステムおよび方法 - Google Patents
連続流動サンプリングプローブを使用する分析器具の音響装填のためのシステムおよび方法 Download PDFInfo
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Abstract
Description
を含む。
式中、Sreagは、試薬ブランクに関する信号であり、σreagは、試薬ブランクの信号に関する既知の標準偏差である。
式中、Sreagおよびσreagは、上記のように定義される。
(実験)
(実施例1)
(実施例2)
(実施例3)
(実施例4)
Claims (55)
- 流体サンプル中の分析物を分析器具に輸送するための音響装填システムであって、前記システムは、
(a)分析物を含む流体サンプルを格納しているリザーバであって、前記流体サンプルは、流体表面を有する、リザーバと、
(b)前記流体表面から前記流体サンプルの液滴を出射するために効果的な様式で音響放射を発生させるための音響液滴エジェクタと、
(c)前記流体表面から間隔を置かれた連続流動サンプリングプローブと
を備え、
前記連続流動サンプリングプローブは、
(i)前記流体サンプルの出射された液滴を受け取るためのサンプリング先端と、
(ii)溶媒源から溶媒を受け取るための溶媒入口と、
(iii)前記溶媒入口から前記サンプリング先端に前記溶媒を輸送するための溶媒輸送毛細管であって、前記出射された液滴は、前記溶媒と結合し、分析物−溶媒希釈液を形成する、溶媒輸送毛細管と、
(iv)サンプル出口であって、前記分析物−溶媒希釈液は、前記サンプル出口を通して前記サンプリングプローブから離れて分析器具に向けられる、サンプル出口と、
(v)前記分析物−溶媒希釈液を前記サンプリング先端から前記サンプル出口に輸送するためのサンプル輸送毛細管と
備え、
前記サンプル輸送毛細管と前記溶媒輸送毛細管とは、前記サンプリング先端において流体連通している、システム。 - 前記連続流動サンプリングプローブは、外側毛細管と、前記外側毛細管の中に同軸に配置された内側毛細管とを備え、前記外側毛細管および内側毛細管は、前記内側毛細管と外側毛細管との間に前記溶媒輸送毛細管を画定し、前記内側毛細管は、前記サンプル輸送毛細管を画定する、請求項1に記載のシステム。
- 前記音響液滴エジェクタは、音響放射発生器と集束手段とを備え、前記集束手段は、発生させられた前記音響放射を前記リザーバ内の流体サンプルの前記表面の近傍の焦点に集束させる、請求項1に記載のシステム。
- 前記音響液滴エジェクタは、前記リザーバと音響結合関係にある、請求項3に記載のシステム。
- 各々が分析物を含む流体サンプルを格納している複数のリザーバを備え、前記流体サンプルのうちの任意のものは、前記流体サンプルのうちの別のものと同一であることも、異なることもある、請求項1に記載のシステム。
- 前記エジェクタを前記リザーバの各々に対して音響結合関係に連続して位置付ける手段をさらに含む、請求項5に記載のシステム。
- 前記リザーバは、アレイにおいて配置されている、請求項5に記載のシステム。
- 前記リザーバは、統合された複数リザーバユニットを備えている基板内に含まれている、請求項7に記載のシステム。
- 前記サンプリング先端に対する前記基板の空間関係を変更する手段をさらに備えている、請求項8に記載のシステム。
- 前記流体サンプルは、約1μL以下の体積を占める、請求項9に記載のシステム。
- 前記流体サンプルは、約10pL〜約100nLの体積を占める、請求項10に記載のシステム。
- 前記音響液滴エジェクタは、約3nL以下の体積を有する液滴を出射するように構成されている、請求項1に記載のシステム。
- 前記音響液滴エジェクタは、約1pL以下の体積を有する液滴を出射するように構成されている、請求項12に記載のシステム。
- 前記溶媒入口に動作可能に接続され、前記溶媒入口と流体連通している溶媒ポンプをさらに含み、前記溶媒ポンプは、前記溶媒輸送毛細管内の溶媒流量を制御する、請求項1に記載のシステム。
- ガス入口をさらに含み、ネブライジングガスが、ガス源から前記ガス入口を通って前記サンプル出口に流動し、それによって、前記サンプル出口から前記分析物−溶媒希釈液を吸引する、請求項1に記載のシステム。
- 前記ネブライジングガス流を制御するために前記ガス入口に動作可能に接続されたガス圧力調整器をさらに含む、請求項15に記載のシステム。
- 前記出口から退出する前記分析物−溶媒希釈液中の分析物をイオン化するためのイオン化源をさらに含む、請求項1に記載のシステム。
- 前記イオン化源は、エレクトロスプレーイオン源である、請求項17に記載のシステム。
- 前記分析器具は、質量分析計を備えている、請求項1に記載のシステム。
- 前記分析器具は、質量分析計を備えている、請求項18に記載のシステム。
- 前記内側毛細管および前記外側毛細管のうちの一方を他方に対して縦方向に移動させるように適合された調節器をさらに含む、請求項1に記載のシステム。
- 分析物を含む流体サンプルを分析器具に輸送する方法であって、前記方法は、
(a)音響放射を発生させる音響液滴エジェクタを流体表面を有する前記流体サンプルを含むリザーバに音響的に結合することと、
(b)前記音響エジェクタを作動させることによって、前記流体サンプルの液滴を前記流体表面から連続流動サンプリングプローブのサンプリング先端の中に出射するために効果的な様式で前記リザーバに向かい前記流体サンプルの中に入る音響放射を発生させることであって、前記出射された液滴は、前記流動プローブ内の循環溶媒と結合し、分析物−溶媒希釈液を形成し、前記サンプリングプローブは、前記流体表面と前記サンプリング先端との間に間隙を提供するために前記流体表面から間隔を置かれている、ことと、
(c)溶媒中の前記受け取られた流体サンプル液滴を前記サンプリングプローブ内のサンプル輸送毛細管を通してサンプル出口に輸送することであって、前記分析物−溶媒希釈液は、前記サンプリングプローブから離れて分析器具に向けられる、ことと
を含む、方法。 - 前記連続流動サンプリングプローブは、溶媒源から前記溶媒を受け取るための溶媒入口と、前記溶媒入口から前記サンプリング先端に前記溶媒を輸送するための溶媒輸送毛細管とを備えている、請求項22に記載の方法。
- 前記サンプル輸送毛細管および前記溶媒輸送毛細管は、前記サンプリング先端において流体連通している、請求項23に記載の方法。
- 前記連続流動サンプリングプローブは、ガス入口を備えている、請求項24に記載の方法。
- ステップ(c)は、ネブライジングガスをガス源からガス入口を通して前記サンプル出口に流動させ、前記サンプル出口において前記分析物−溶媒希釈液を吸引することを含む、請求項25に記載の方法。
- 前記ガス入口に動作可能に接続されたガス圧力調整器を用いて、前記サンプル輸送毛細管内のサンプル流量を制御することをさらに含む、請求項26に記載の方法。
- 前記溶媒入口に動作可能に接続され、前記溶媒入口と流体連通している溶媒ポンプを用いて、前記溶媒輸送毛細管内の溶媒流量を制御することをさらに含む、請求項27に記載の方法。
- 前記サンプル流量に対して前記溶媒流量を調節し、前記溶媒輸送毛細管と前記サンプル輸送毛細管とが流体連通している前記サンプリング先端において所望の流動パターンを提供することをさらに含む、請求項28に記載の方法。
- 前記所望の流動パターンは、渦である、請求項29に記載の方法。
- 前記渦は、超臨界渦である、請求項30に記載の方法。
- 前記リザーバは、各々が分析物を含む流体サンプルを格納している複数のリザーバのうちの1つであり、前記リザーバ内の前記流体サンプルは、同一であることも、異なることもある、請求項22に記載の方法。
- 前記リザーバは、アレイにおいて配置されている、請求項32に記載の方法。
- 前記リザーバは、統合された複数リザーバユニットを備えている基板内に含まれている、請求項33に記載の方法。
- 前記液滴を出射した後、前記エジェクタを別のリザーバに対して音響結合関係に位置付け、ステップ(b)および(c)を繰り返すことをさらに含む、請求項32に記載の方法。
- 前記サンプル出口から退出する前記分析物希釈液中の前記分析物をイオン化することをさらに含む、請求項22に記載の方法。
- 前記分析器具は、質量分析計を備えている、請求項22に記載の方法。
- 前記分析器具は、質量分析計を備えている、請求項36に記載の方法。
- 前記分析物は、約100ダルトン〜約100キロダルトンの範囲内の分子量を有する化合物を含む、請求項22に記載の方法。
- 前記分子量は、約1〜約100キロダルトンである、請求項39に記載の方法。
- 前記分析物は、非金属である、請求項40に記載の方法。
- 前記分析物は、有機化合物を含む、請求項41に記載の方法。
- 前記有機化合物は、生体分子である、請求項42に記載の方法。
- 前記生体分子は、ペプチド性である、請求項43に記載の方法。
- 前記流体サンプルは、約10pL以下の体積を占める、請求項22に記載の方法。
- 前記流体サンプルは、約1pL以下の体積を占める、請求項45に記載のシステム。
- 前記流体サンプルは、約10pL〜約100nLの体積を占める、請求項46に記載のシステム。
- 前記音響的に出射された液滴は、約3nL以下の体積を有する、請求項22に記載の方法。
- 前記音響的に出射された液滴は、約1pL以下の体積を有する、請求項48に記載の方法。
- ステップ(a)および(b)を迅速に繰り返し、前記流体サンプルの1回の注入として複数の超単分散液滴を前記サンプリング先端の中に出射することを含む、請求項22に記載の方法。
- ステップ(a)および(b)を迅速に繰り返し、前記流体サンプルの1回の注入として複数の超単分散液滴を前記サンプリング先端の中に出射することを含む、請求項48に記載の方法。
- ステップ(a)および(b)は、少なくとも約250Hzの周波数において繰り返される、請求項51に記載の方法。
- 前記流体サンプルは、溶媒中の分析物を含む、請求項22に記載の方法。
- 前記流体サンプルは、生物学的流体サンプルを含む、請求項22に記載の方法。
- 前記生物学的流体サンプルは、組織、組織ホモジネート、細胞、細胞懸濁液、細胞抽出液、全血、血漿、漿液、唾液、痰、鼻汁、脳脊髄液、間質液、リンパ液、精液、膣液、または糞便を含む、請求項54に記載の方法。
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Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US10770277B2 (en) * | 2017-11-22 | 2020-09-08 | Labcyte, Inc. | System and method for the acoustic loading of an analytical instrument using a continuous flow sampling probe |
CN108548700B (zh) * | 2018-03-16 | 2019-07-23 | 华中科技大学 | 一种无水冷高温气溶胶定量稀释取样探头 |
WO2020242911A1 (en) * | 2019-05-31 | 2020-12-03 | Purdue Research Foundation | Integrated microfluidic probe (imfp) and methods of use thereof |
CN110806384A (zh) * | 2019-12-06 | 2020-02-18 | 天津师范大学 | 一种分析仪器进样装置及使用方法 |
JP7430798B2 (ja) * | 2019-12-19 | 2024-02-13 | ラジオメーター・メディカル・アー・ペー・エス | 多孔質膜センサ組立体 |
WO2021144713A1 (en) * | 2020-01-14 | 2021-07-22 | Dh Technologies Development Pte. Ltd. | Method of mass analysis –controlling viscosity of solvent for opp operation |
WO2021168034A1 (en) * | 2020-02-18 | 2021-08-26 | Benubio | Method and apparatus for large-scale spheroid generation |
CN115668442A (zh) * | 2020-05-22 | 2023-01-31 | Dh科技发展私人贸易有限公司 | 用于开放端口采样探头的溢出传感器 |
EP4154296A1 (en) * | 2020-05-22 | 2023-03-29 | DH Technologies Development Pte. Ltd. | Simplification of method or system using scout mrm |
CN115699247A (zh) * | 2020-05-22 | 2023-02-03 | Dh科技发展私人贸易有限公司 | 用于增加通量的方法 |
US20230207298A1 (en) * | 2020-05-22 | 2023-06-29 | Dh Technologies Development Pte. Ltd. | AEMS Auto Tuning |
WO2021234644A1 (en) * | 2020-05-22 | 2021-11-25 | Dh Technologies Development Pte. Ltd. | Identification of a first sample in a series of sequential samples |
EP4158306A2 (en) * | 2020-05-25 | 2023-04-05 | DH Technologies Development Pte. Ltd. | Mass analysis |
US20230377867A1 (en) * | 2020-10-13 | 2023-11-23 | Dh Technologies Development Pte. Ltd. | Exhaust Flow Boosting for Sampling Probe for Use in Mass Spectrometry Systems and Methods |
EP4252269A1 (en) | 2020-11-30 | 2023-10-04 | DH Technologies Development Pte. Ltd. | System and methods for segmented flow analysis |
US20240112901A1 (en) | 2020-12-21 | 2024-04-04 | Dh Technologies Development Pte. Ltd. | Systems and methods for controlling flow through an open port interface |
CN116724376A (zh) * | 2020-12-22 | 2023-09-08 | Dh科技发展私人贸易有限公司 | 声学喷射质谱法的动态喷射延迟时间 |
WO2022136911A1 (en) * | 2020-12-23 | 2022-06-30 | Dh Technologies Development Pte. Ltd. | Method and system for timed introduction of sample into a mass spectrometer |
CN116711049A (zh) * | 2020-12-28 | 2023-09-05 | Dh科技发展私人贸易有限公司 | 用于质谱法信号质量评估的方法与系统 |
WO2022157668A1 (en) * | 2021-01-20 | 2022-07-28 | Dh Technologies Development Pte. Ltd. | Electrode protrusion adjustment for maximizing pressure drop across liquid transport conduit |
US20220236249A1 (en) * | 2021-01-25 | 2022-07-28 | Koninklijke Philips N.V. | Apparatus and method for sputum conditioning and analysis |
EP4288991A1 (en) | 2021-02-02 | 2023-12-13 | DH Technologies Development Pte. Ltd. | Methods and apparatus for washing sampling probe for use in mass spectrometry systems |
US20240079225A1 (en) | 2021-02-03 | 2024-03-07 | Dh Technologies Development Pte. Ltd. | Liquid flow/air flow combination for sample transport |
US20240096611A1 (en) * | 2021-02-09 | 2024-03-21 | Dh Technologies Development Pte. Ltd. | Systems and methods for obtaining samples for analysis |
CN116917728A (zh) | 2021-02-24 | 2023-10-20 | Dh科技发展私人贸易有限公司 | 差分迁移率谱仪单元的动态加热 |
US20240175787A1 (en) | 2021-02-25 | 2024-05-30 | Dh Technologies Development Pte. Ltd. | Systems and methods for sampling |
CN117098991A (zh) * | 2021-03-19 | 2023-11-21 | Dh科技发展私人贸易有限公司 | 用于液相色谱系统的具有开放端口接口的非接触式采样器 |
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WO2023012762A1 (en) * | 2021-08-05 | 2023-02-09 | Dh Technologies Development Pte. Ltd. | Standard addition workflow for quantitative analysis |
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WO2024127358A1 (en) | 2022-12-16 | 2024-06-20 | Dh Technologies Development Pte. Ltd. | Systems and methods for reducing data storage requirements in mass analysis systems |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020109084A1 (en) * | 2001-02-14 | 2002-08-15 | Ellson Richard N. | Acoustic sample introduction for mass spectrometric analysis |
US20100224013A1 (en) * | 2009-03-05 | 2010-09-09 | Van Berkel Gary J | Method and system for formation and withdrawal of a sample from a surface to be analyzed |
US20160299041A1 (en) * | 2015-04-09 | 2016-10-13 | Ut-Battelle, Llc | Capture Probe |
Family Cites Families (38)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4308547A (en) | 1978-04-13 | 1981-12-29 | Recognition Equipment Incorporated | Liquid drop emitter |
US5041849A (en) | 1989-12-26 | 1991-08-20 | Xerox Corporation | Multi-discrete-phase Fresnel acoustic lenses and their application to acoustic ink printing |
ATE182336T1 (de) | 1993-05-28 | 1999-08-15 | Chiron Corp | Peptidinhibitoren der urokinaserezeptor-aktivität |
JPH08254446A (ja) | 1995-03-16 | 1996-10-01 | Fujitsu Ltd | 超音波印字方法,超音波印字装置及び音響レンズの成形方法 |
US5587582A (en) | 1995-05-19 | 1996-12-24 | Cornell Research Foundation, Inc. | Self-aligning liquid junction |
US7527357B2 (en) | 1997-07-15 | 2009-05-05 | Silverbrook Research Pty Ltd | Inkjet nozzle array with individual feed channel for each nozzle |
JP4038310B2 (ja) | 1999-09-30 | 2008-01-23 | 日産自動車株式会社 | 無段変速機のセレクト時ライン圧制御装置 |
WO2001032245A1 (en) | 1999-11-03 | 2001-05-10 | Cornell Research Foundation, Inc. | Miniaturized fluid transfer device |
US6746104B2 (en) | 2000-09-25 | 2004-06-08 | Picoliter Inc. | Method for generating molecular arrays on porous surfaces |
US6666541B2 (en) | 2000-09-25 | 2003-12-23 | Picoliter Inc. | Acoustic ejection of fluids from a plurality of reservoirs |
US7900505B2 (en) | 2000-09-25 | 2011-03-08 | Labcyte Inc. | Acoustic assessment of fluids in a plurality of reservoirs |
US6893836B2 (en) | 2000-11-29 | 2005-05-17 | Picoliter Inc. | Spatially directed ejection of cells from a carrier fluid |
US6849423B2 (en) | 2000-11-29 | 2005-02-01 | Picoliter Inc | Focused acoustics for detection and sorting of fluid volumes |
US6855925B2 (en) * | 2001-02-14 | 2005-02-15 | Picoliter Inc. | Methods, devices, and systems using acoustic ejection for depositing fluid droplets on a sample surface for analysis |
US6416164B1 (en) | 2001-07-20 | 2002-07-09 | Picoliter Inc. | Acoustic ejection of fluids using large F-number focusing elements |
US20030101819A1 (en) * | 2001-12-04 | 2003-06-05 | Mutz Mitchell W. | Acoustic assessment of fluids in a plurality of reservoirs |
US7354141B2 (en) | 2001-12-04 | 2008-04-08 | Labcyte Inc. | Acoustic assessment of characteristics of a fluid relevant to acoustic ejection |
US7717544B2 (en) | 2004-10-01 | 2010-05-18 | Labcyte Inc. | Method for acoustically ejecting a droplet of fluid from a reservoir by an acoustic fluid ejection apparatus |
CA2469931A1 (en) * | 2001-12-11 | 2003-07-03 | Astrazeneca Ab | Machine and method for processing biomolecules |
US6932097B2 (en) | 2002-06-18 | 2005-08-23 | Picoliter Inc. | Acoustic control of the composition and/or volume of fluid in a reservoir |
US6827287B2 (en) * | 2002-12-24 | 2004-12-07 | Palo Alto Research Center, Incorporated | High throughput method and apparatus for introducing biological samples into analytical instruments |
US7070260B2 (en) | 2003-01-09 | 2006-07-04 | Labcyte Inc. | Droplet dispensation from a reservoir with reduction in uncontrolled electrostatic charge |
US20060210443A1 (en) * | 2005-03-14 | 2006-09-21 | Stearns Richard G | Avoidance of bouncing and splashing in droplet-based fluid transport |
DE102006056929B4 (de) | 2006-12-04 | 2010-09-02 | Bruker Daltonik Gmbh | Massenspektrometrie mit Laser-Ablation |
JP5626889B2 (ja) | 2007-09-19 | 2014-11-19 | ディーエイチ テクノロジーズ デベロップメント プライベート リミテッド | 質量分析計用衝突セル |
US8107319B2 (en) | 2009-12-03 | 2012-01-31 | Labcyte Inc. | Acoustic fluid height monitoring using dynamic surface perturbations |
WO2011094310A2 (en) | 2010-01-26 | 2011-08-04 | Labcyte Inc. | Focus-activated acoustic ejection |
US20140166875A1 (en) | 2010-09-02 | 2014-06-19 | Wayne State University | Systems and methods for high throughput solvent assisted ionization inlet for mass spectrometry |
US8486703B2 (en) * | 2010-09-30 | 2013-07-16 | Ut-Battelle, Llc | Surface sampling concentration and reaction probe |
US10068200B1 (en) * | 2011-01-22 | 2018-09-04 | Mass Insight, Inc. | Business and data processing system for providing mass spectrometric services |
GB201120141D0 (en) | 2011-11-22 | 2012-01-04 | Micromass Ltd | Low cross-talk (cross-contamination) fast sample delivery system based upon acoustic droplet ejection |
GB2512309A (en) * | 2013-03-25 | 2014-10-01 | Thermo Electron Mfg Ltd | Apparatus and method for liquid sample introduction |
GB2512308B (en) * | 2013-03-25 | 2016-07-06 | Thermo Electron Mfg Ltd | Apparatus and method for liquid sample introduction using acoustic droplet generator |
DE112015004694B4 (de) | 2014-10-17 | 2022-10-06 | Micromass Uk Limited | Verfahren zum Ionisieren einer Probe und Ionenquelle |
US9632066B2 (en) | 2015-04-09 | 2017-04-25 | Ut-Battelle, Llc | Open port sampling interface |
US10325768B1 (en) * | 2015-09-03 | 2019-06-18 | Labcyte Inc. | Focused acoustic radiation for rapid sequential ejection of subwavelength droplets |
GB201522594D0 (en) | 2015-12-22 | 2016-02-03 | Micromass Ltd | Secondary ultrasonic nebulisation |
US10770277B2 (en) * | 2017-11-22 | 2020-09-08 | Labcyte, Inc. | System and method for the acoustic loading of an analytical instrument using a continuous flow sampling probe |
-
2018
- 2018-11-21 US US16/198,667 patent/US10770277B2/en active Active
- 2018-11-21 EP EP18881164.0A patent/EP3714263A4/en active Pending
- 2018-11-21 AU AU2018374058A patent/AU2018374058A1/en not_active Abandoned
- 2018-11-21 CA CA3081369A patent/CA3081369A1/en active Pending
- 2018-11-21 JP JP2020528057A patent/JP7307062B2/ja active Active
- 2018-11-21 WO PCT/US2018/062337 patent/WO2019104235A1/en unknown
- 2018-11-21 US US16/198,759 patent/US20190157061A1/en not_active Abandoned
- 2018-11-21 CN CN201880075488.0A patent/CN111630378A/zh active Pending
- 2018-11-22 WO PCT/US2018/062359 patent/WO2019104248A1/en active Application Filing
-
2020
- 2020-08-04 US US16/985,076 patent/US11133163B2/en active Active
-
2021
- 2021-08-24 US US17/410,758 patent/US11637006B2/en active Active
-
2023
- 2023-02-01 JP JP2023013893A patent/JP2023041830A/ja not_active Withdrawn
- 2023-03-14 US US18/121,550 patent/US20230290627A1/en active Pending
- 2023-11-21 AU AU2023270229A patent/AU2023270229A1/en active Pending
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20020109084A1 (en) * | 2001-02-14 | 2002-08-15 | Ellson Richard N. | Acoustic sample introduction for mass spectrometric analysis |
US20100224013A1 (en) * | 2009-03-05 | 2010-09-09 | Van Berkel Gary J | Method and system for formation and withdrawal of a sample from a surface to be analyzed |
US20160299041A1 (en) * | 2015-04-09 | 2016-10-13 | Ut-Battelle, Llc | Capture Probe |
Non-Patent Citations (2)
Title |
---|
BERKEL, GARY J VAN ET AL.: "Immediate drop on demand technology (I-DOT) coupled with mass spectrometry via an open port sampling", BIOANALYSIS, vol. 9, no. 21, JPN6022003715, 2 November 2017 (2017-11-02), GB, pages 1667 - 1679, XP055551482, ISSN: 0004885729, DOI: 10.4155/bio-2017-0104 * |
SINCLAIR, IAN ET AL.: "Novel Acoustic Loading of a Mass Spectrometer: Toward Next-Generation High-Throughput MS Screening", JOURNAL OF LABORATORY AUTOMATION, vol. 21, no. 1, JPN6022003712, 2016, US, pages 19 - 26, XP055552667, ISSN: 0004885730, DOI: 10.1177/2211068215619124 * |
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JP2023041830A (ja) | 2023-03-24 |
US20190157060A1 (en) | 2019-05-23 |
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