JP2021185824A - Ribonucleic acid detection panel and ribonucleic acid detection device - Google Patents

Abstract

To provide a ribonucleic acid detection panel and a ribonucleic acid detection device.SOLUTION: Provided are a ribonucleic acid detection panel and a ribonucleic acid detection device, in which the ribonucleic acid detection device includes a control unit and a ribonucleic acid detection panel, the ribonucleic acid detection panel includes a substrate, a plurality of detection electrode layers electrically connected to the control unit, at least one primer layer, and a plurality of trace layers. The plurality of detection electrode layers is provided on a first surface of the substrate, the at least one primer layer is provided on the plurality of detection electrode layers, insulated from each other, and the plurality of electrode trace layers is electrically connected to the plurality of detection electrodes and the control unit. The design of the present invention exerts an effect of shortening the detection time and cost reduction.SELECTED DRAWING: Figure 1

Description

The present invention relates to an ribonucleic acid detection panel and a ribonucleic acid detection device, and more particularly to a ribonucleic acid detection panel and a ribonucleic acid detection device that can realize shortening of detection time and cost reduction.

Currently, if you are trying to detect a user's disease (Kawasaki disease, colorectal cancer, hand and foot disease, new coronavirus (COVID-19) or other virus with RNA), send it to the measurement platform of a medical institution and label it ( After (such as a polymerase chain reaction (PCR) analyzer), the user's blood is then taken, the miRNA virus is extracted via an RNA extractor, and in turn miRNA is added, miRNA labeling reagents are added, and miRNA. Staining and scanning are performed by dropping on the chip, and the presence or absence of a definitive diagnosis of the user is determined by the change in fluorescence intensity and data comparison. Therefore, conventionally, when detecting RNA (or miRNA) virus, specialized equipment (measurement platform, RNA extraction device, etc.) is required, and only specific specialized medical personnel operate these specialized equipment. There was a problem that the difficulty was high and the cost was high. Further, since the conventional virus detection procedure using a fluorescent label is complicated, it takes at least 2 hours or more or 2 days or more to detect the disease, and there is a problem that the detection time is long.

An object of the present invention is to provide a ribonucleic acid detection panel capable of shortening the detection time and reducing the cost.

Another object of the present invention is to provide a ribonucleic acid detection panel which is easy to detect and has excellent usability at the time of detection.

Yet another object of the present invention is to provide a ribonucleic acid detection apparatus capable of shortening the detection time and reducing the cost.

Yet another object of the present invention is to provide a ribonucleic acid detecting apparatus having a simple detection operation and excellent usability at the time of detection.

To achieve the above object, the present invention provides a ribonucleic acid detection panel comprising a substrate, a plurality of detection electrode layers, at least one primer layer, and a plurality of electrode trace layers. The substrate has a first surface and a second surface on the opposite side thereof, the plurality of detection electrode layers are provided on the first surface, and the at least one primer layer is the plurality of detection electrodes. The plurality of electrode trace layers are provided on the first surface and are electrically connected to the plurality of detection electrodes.

The present invention further provides a ribonucleic acid detector comprising a control unit and a ribonucleic acid detection panel. The ribonucleic acid detection panel includes a substrate, a plurality of detection electrode layers, at least one primer layer, and a plurality of electrode trace layers, and the substrate is a first surface and a second surface on the opposite side thereof. It has a surface, the plurality of detection electrode layers are electrically connected to the control unit, are provided on the first surface, and the at least one primer layer is provided on the plurality of detection electrode layers. Used for reaction with a sample having ribonucleic acid and isolated from each other, the plurality of electrode trace layers are provided on the first surface and are electrically connected to the plurality of detection electrode layers and the control unit.

Therefore, in each of the above-described embodiments of the present invention, the design of the present invention can shorten the detection time and reduce the cost, and the user can promptly detect the detection by himself / herself. Not only the detection operation is simple, but also the detection operation is simple. Moreover, detection is also very convenient.

It is a three-dimensional decomposition view of the ribonucleic acid detection panel which concerns on Example 1 of this invention. It is a three-dimensional assembly drawing of the ribonucleic acid detection panel which concerns on Example 1 of this invention. It is sectional drawing and partial enlarged view of the ribonucleic acid detection panel which concerns on Example 1 of this invention. FIG. 3 is a cross-sectional view and a partially enlarged view of an example of the ribonucleic acid detection panel according to the first embodiment of the present invention. It is a three-dimensional decomposition view of the ribonucleic acid detection apparatus which concerns on Example 2 of this invention. It is 1st aspect 3D assembly drawing of the ribonucleic acid detection apparatus which concerns on Example 2 of this invention. It is a top view of FIG. 4A of Example 2 of this invention. It is a 2D aspect 3D assembly drawing of the ribonucleic acid detection apparatus which concerns on Example 2 of this invention. It is a 3D assembly drawing of the 3rd aspect of the ribonucleic acid detection apparatus which concerns on Example 2 of this invention. It is a detection result chart of the primer layer corresponding to the sample which concerns on Example 2 of this invention.

The above object, structure and functional features of the present invention will be described based on preferred embodiments of the accompanying drawings.

The present invention provides a ribonucleic acid detection panel and a ribonucleic acid detection device. Referring to FIGS. 1 to 2C, FIG. 1 is a three-dimensional decomposition view of the ribonucleic acid detection panel according to the first embodiment of the present invention; FIG. 2A is a three-dimensional assembly of the ribonucleic acid detection panel according to the first embodiment of the present invention. FIG. 2B is a cross-sectional view and a partially enlarged view of the ribonucleic acid detection panel according to the first embodiment of the present invention; FIG. 2C is an embodiment of the ribonucleic acid detection panel according to the first embodiment of the present invention. It is a cross-sectional view and a partially enlarged view. As shown in FIGS. 1, 2A and 2B, the ribonucleic acid (RNA) detection panel 1 includes a substrate 11, a plurality of detection electrode layers, at least one primer layer 115, and a plurality of electrode trace layers. The substrate 11 is a glass substrate 11, a circuit board 22 (for example, a flexible circuit board) or a polyethylene terephthalate (PET) substrate 11, and the substrate 11 in this embodiment is described as the glass substrate 11. However, the substrate 11 is not limited to this, and the substrate 11 has a first surface 111 and a second surface 112 on the opposite side thereof, and the first surface 111 of the substrate 11 has a detection region 1111 and the detection region. A peripheral region 1112 surrounding the 1111 is provided, the plurality of detection electrode layers are provided on the first surface 111 of the detection region 1111, and the plurality of electrode trace layers 116 are provided on the first surface of the peripheral region 1112. Provided on the 111, the plurality of electrode trace layers 116 are electrically connected to the plurality of detection electrode layers.

The plurality of detection electrode layers include a transparent or opaque first detection electrode layer 113 and a transparent or opaque second detection electrode layer 114, and the first detection electrode layer 113 is included in the detection region 1111. A plurality of first detection electrodes 1131 (for example, X-axis detection electrodes) provided on the first surface 111 are provided, and the second detection electrode layer 114 is provided on the first detection electrode layer 113. 2 The detection electrode 1141 (for example, a Y-axis detection electrode) is provided. In this embodiment, the plurality of first and second detection electrodes 1131 and 1141 are represented as an X-axis detection electrode and a Y-axis detection electrode, respectively, and are arranged at an intersection. The material of the plurality of detection electrode layers and the plurality of electrode trace layers 116 is a metal material, and the metal material is, for example, indium tin oxide (ITO), indium zinc oxide (indium zinc oxide, ITO), and the like. IZO), antimony-doped tin oxide (ATO), or a combination thereof, but is not limited thereto. When specifically implemented, the metal material may be aluminum (Al), gold (Au), copper, silver (Ag) or other metal material (such as nickel (Ni), chromium (Cr) or an alloy of the metal). May be. In this embodiment, the shapes of the plurality of first and second detection electrodes 1131 and 1141 are rhombic, but the shape is not limited thereto. In one embodiment, the shape of the plurality of first and second detection electrodes 1131, 1141 may be strip-shaped, stick-shaped, wide strip-shaped, or rectangular. In another embodiment, the plurality of first detection electrodes 1131 are arranged in a matrix array (M × N array or the like) on the first surface 111 in the detection region 1111, and the plurality of second detection electrodes 1141 are arranged. Are arranged on the first detection electrode layer 113 in a matrix array (M × N array or the like), and the plurality of first and second detection electrodes 1131 and 1141 are isolated from each other.

In an alternative embodiment, the plurality of first and second detection electrodes 1131 and 1141 of the first and second detection electrode layers 113 and 114 are parallel to each other and correspond to the first surface 111 of the substrate 11. The primer layer 115 is provided on the first and second detection electrode layers 113 and 114, and is insulated from each other.

The first insulating layer 117 is provided between the first and second detection electrode layers 113 and 114, and the material of the first insulating layer 117 is, for example, silicon dioxide (SiO2). The at least one primer layer 115 is formed on the plurality of detection electrode layers by, for example, printing or a coating method, and the primer layer 115 and the plurality of detection electrode layers are insulated from each other. In this embodiment, the primer layer 115 is shown as a plurality of primer layers 115 arranged at intervals on the second detection electrode layer 114. The primer layer 115 is made of a polymer material and is used to react with a sample having the corresponding ribonucleic acid (or microribonucleic acid microRNA) (such as saliva of a user). For example, if the saliva of a user who does not have the RNA virus (that is, the RNA sample) and the primer layer 115 that does not satisfy the corresponding specificity cannot react with each other and bind to each other, an electrical change (change in capacitance or dielectric constant) is not possible. 1st and 2nd detection electrodes 1131 of the 1st and 2nd detection electrode layers 113 and 114, as if the user's finger was not touched on the detection area 1111. , 1141 has a fixed coupling capacitor, and at this time, the electric field (power line) between the plurality of first and second detection electrodes 1131 and 1141 is fixed. On the other hand, after the primer layer 115 satisfying the corresponding specificity with the saliva of the user carrying the RNA virus (that is, the RNA sample) reacts with each other and binds to each other, there is a clear electrical change (change in capacitance). There is a change in dielectric constant, etc.), and a capacitance is formed between the primer layer 115 and the second detection electrode layer 114 as if the user's finger is touching the detection region 1111. At this time, the electric field (power line) originally steadily distributed between the plurality of first and second detection electrodes 1131 and 1141 of the first and second detection electrode layers 113 and 114 is a primer layer corresponding to some power lines. By connecting to 115, it changes (that is, the electric field changes), and therefore the capacitance value of the coupling capacitor between the plurality of first and second detection electrodes 1131 and 1141 is further changed (that is, electrostatic). The capacity value changes).

Further, the primer layer 115 reacts with and binds to a virus satisfying the corresponding specificity (such as Kawasaki disease, colon cancer, limb and mouth disease, new coronavirus (COVID-19) or other virus having RNA). Primers, such as specific primers, are pre-designed to be used to detect viruses such as the new coronavirus (COVID-19). If there is a new coronavirus (COVID-19) with RNA in the user's saliva (ie, specimen), there is a clear electrical change after reacting with and binding to the primer layer 115 that meets the detection response of the new coronavirus. Indicates that the user has made a definitive diagnosis (in the case of a positive result) of infection with the new coronavirus (COVID-19) (that is, infected with the virus). On the other hand, if the user's saliva (that is, the sample) does not have a virus or other virus (for example, Kawasaki disease), it cannot bind to the primer layer 115 that does not meet the detection response of the new coronavirus even if it reacts with each other. , Indicates that the user does not make a definitive diagnosis of infection with the new coronavirus (in the case of a negative result) (ie, not infected with the virus) in the absence of electrical changes. The primer (or called primer) is a small fragment of single-stranded DNA or RNA, which is an artificially synthesized primer in a natural organism's DNA replication (RNA primer) and polymerase linkage reaction (PCR) as the starting point of DNA replication. It is present in (usually a DNA primer) and consists of nucleotides.

A second insulating layer 118 is provided between the plurality of primer layers 115 and the second detection electrode layer 114, and a third insulating layer 119 is provided between the first detection electrode layer 113 and the substrate 11. The material of the second and third insulating layers 118 and 119 is, for example, silicon dioxide (SiO2). In an alternative embodiment, the installation of the third insulating layer 119 can be omitted, and the first detection electrode layer 113 can be provided on the first surface 111 of the substrate 11 (glass substrate, PET substrate, etc.). ..

The plurality of primer layers 115 are elongated and located in the detection region 1111, and are on the second insulating layer 118 corresponding to the intersection position of the plurality of first detection electrodes 1131 and the plurality of second detection electrodes 1141. Are arranged at intervals in the X-axis direction (or Y-axis direction). In one embodiment, referring to FIG. 2C, a plurality of recesses 1181 are provided on the second insulating layer 118 at positions corresponding to the plurality of primer layers 115, and the plurality of primer layers 115 are provided with the second insulating layer. It is housed in a plurality of recesses 1181 of the layer 118 and defines a microchannel 1182 between each primer layer 115 and the corresponding inner wall of each recess 1181. The microchannel 1182 is used in order to effectively improve the accuracy of detection and shorten the detection time. The microchannel 1182 is used to increase the contact area of the primer layer 115 corresponding to the sample by collecting the sample (for example, saliva).

Therefore, through the design of the ribonucleic acid detection panel 1 of the present invention, the detection time can be shortened and the cost can be reduced, and the user can quickly detect his / her physical condition through his / her saliva. Not only is it easy to operate, but it is also very convenient to use for detection.

With reference to FIGS. 1 and 3 to 6, FIG. 3 is a three-dimensional decomposition view of the ribonucleic acid detection apparatus according to the second embodiment of the present invention; FIG. 4A is the ribonucleic acid detection according to the second embodiment of the present invention. 1st aspect of the apparatus is a three-dimensional assembly drawing; FIG. 4B is a top view of FIG. 4A according to the second embodiment of the present invention; FIG. 5 is a second view of the ribonucleic acid detection apparatus according to the second embodiment of the present invention. FIG. 6 is a third aspect three-dimensional assembly drawing of the ribonucleic acid detection device according to the second embodiment of the present invention. In this embodiment, the ribonucleic acid detection panel 1 of the first embodiment is mainly applied onto the ribonucleic acid detection (RNA) device 2, that is, the ribonucleic acid detection device 2 of the present embodiment is It includes a control unit 21 and an ribonucleic acid detection panel 1. Since the structure, linkage relationship, and effect of the ribonucleic acid (RNA) detection panel 1 in this example are the same as the structure, linkage relationship, and effect thereof of the ribonucleic acid (RNA) detection panel 1 of Example 1. No further explanation is given here. The control unit 21 is a central processing unit (CPU), a microcontroller (MCU), or a digital signal processing processor (DSP). The control unit 21 is electrically connected to the plurality of electrode trace layers 116 and the plurality of detection electrode layers, and received the plurality of detection electrode layers (that is, the plurality of first and second detection electrodes 1131, 1141). Based on the signal of the change in the capacitance value transmitted from the sample, the presence or absence of an electrical change in the sample and the primer layer 115 is determined, and a detection result is generated. The detection result is the result when the sample and the primer layer 115 have an electrical change (change in the capacitance value), or the sample and the primer layer 115 have an electrical change (there is no change in the capacitance value). This is the result when there is no.

The control unit 21 of this embodiment has three modes of installation, the first mode of which is shown in FIGS. 3, 4A and 4B. In the first aspect, the control unit 21 is provided on the first surface 111 of the peripheral region 1112 of the substrate 11 (glass substrate 11 or PET substrate 11 or the like), and the control unit 21 is a chip-on-glass mounting method (chip). -On-glass, COG) is bonded to the first surface 111 of the substrate 11. The control unit 21 is electrically connected to a circuit board 22 (flexible circuit board 22, FPC, etc.), and the circuit board 22 is joined and integrated with the board 11 by a thermocompression bonding method. The circuit board 22 is provided with a display element 23, a processing unit 24, a wireless transmission / reception unit 25, and a power supply unit 26, and the processing unit 24 is electrically connected to the control unit 21, the display element 23, and the wireless transmission / reception unit 25. Has been done. The processing unit 24 is, for example, a central processing unit (CPU), a digital signal processing processor (DSP) or a controller (MCU), and is used for processing and executing signals. For example, the processing unit 24 processes that there is an electrical change based on the detection result transmitted from the control unit 21 to generate detection result information, and the detection result information (positive determination) via the display element 23. Result) is displayed. On the other hand, the processing unit 24 processes that there is no electrical change based on the detection result transmitted from the control unit 21 to generate the detection result information, and the detection result information (the detection result information () via the display element 23. Negative confirmation result) is displayed. In this way, it is possible to notify the user himself whether or not he / she has been infected with a virus from the display element 23.

In this embodiment, the display element 23 is shown as a display for displaying detection result information, but the present invention is not limited to this, and in other embodiments, the display element 23 is a plurality of light emitting diodes (LEDs). ), That is, the detection result is shown through a plurality of indicator lights, for example, the lighting of the red LED light shows the positive diagnosis result, and the blue LED light shows the negative diagnosis result. In this embodiment, the power supply unit 26 is a battery and is used to supply power to the display element 23, the processing unit 24, the wireless transmission / reception unit 25, and the ribonucleic acid detection panel 1. In one embodiment, the power supply unit 26 may be a rechargeable battery, and the circuit board 22 is provided with a port (such as a Micro USB port) for charging the power supply unit 26.

In this embodiment, the wireless transmission / reception unit 25 is a Bluetooth (registered trademark) unit (such as a Bluetooth (registered trademark) transmitter / receiver). The Bluetooth® unit is wirelessly linked to an electronic device (such as a smartphone, smartwatch, computer, laptop or tablet computer; not shown) and the processing unit 24 is through the Bluetooth® unit. It is used to wirelessly transmit and display the detection result information to the electronic device. Specifically, the radio transmission / reception unit 25 may be a Wi-Fi unit or an RF (radio frequency) unit.

The second aspect is shown in FIG. 5, and the difference between the second aspect and the first aspect is that the control unit 21 is provided on the circuit board 22, and the circuit board is provided by a flip chip mounting method (COF). It is to join to 22. On the other hand, the third aspect is shown in FIG. 6, and the difference between the third aspect and the above-mentioned second aspect is that the substrate 11 is a circuit board instead of the circuit board 22 to which the substrate 11 is bonded to the outside of the second aspect. (Or a PET substrate), the control unit 21, the processing unit 24, the display element 23, the wireless transmission / reception unit 25, and the power supply unit 26 are provided together in the peripheral region 1112 on the same substrate 11 (or PET substrate 11). Moreover, the detection region 1111 on the substrate 11 approaches the central position of the substrate 11.

When a user attempts to detect a Kawasaki disease (or mucocutaneous Lymph Node Syndrome) virus, the user drops his saliva (ie, specimen) into the detection area 1111. When saliva contains a Kawasaki disease virus miRNA selected from miR-30e-3P, the Kawasaki disease virus miRNA reacts and binds with the corresponding primer layer 115 for detecting the Kawasaki disease virus. Then, after reacting with and binding to the primer layer 115 corresponding to Kawasaki disease virus detection, there is a clear electrical change (change in capacitance, etc.), and the control unit 21 detects the plurality of first and second. Based on the change signal of the capacitance value transmitted from the electrodes 1131 and 1141, the electrical change of the sample and the primer layer 115 is determined, the detection result is generated and transmitted to the processing unit 24, and the processing unit is transmitted. 24 is processed to have an electrical change based on the detection result, the detection result information is transmitted to the display element 23, and the user himself / herself can know through the detection result information displayed by the display element 23. (Fig. 7). On the other hand, if the miRNA in the user's saliva is miR-223-3P (which does not have the miRNA Kawasaki disease virus) and cannot react with and bind to the primer layer 115 corresponding to Kawasaki disease virus detection, then there is obvious electricity. The processing unit 24 is processed to have no electrical change based on the detection result transmitted from the control unit 21, the detection result information is transmitted, and the detection displayed by the display element 23 is performed. The user can be notified via the result information (FIG. 7). FIG. 7 is a chart of the detection results of the primer layer corresponding to the sample, and in the chart, the value of the detection result (32.5) corresponding to the sample miR-30e-3P is the detection result corresponding to the sample miR-223-3P. Greater than the value (6.2). When the reference value set in advance for the value of the detection result in this embodiment is 15, that is, when the value of the detection result is 15 or more, a definitive diagnosis is shown, and the larger the value of the detection result, the more the user. It indicates that the number of days that the virus is in the body is getting longer and the symptoms are getting worse. On the other hand, if the value of the detection result is less than 15, it indicates that the diagnosis is not confirmed.

Therefore, through the design of disease detection by the electrical measurement method of the ribonucleic acid detection device 2 of the present invention, the detection time can be effectively shortened, the disease (for example, a disease virus) can be detected within 15 minutes, and the cost can be reduced. Moreover, the user can detect it immediately at home, and not only the detection operation is easy, but also it is very convenient to use at the time of detection.

1 Ribonucleic acid detection panel 11 Substrate 111 First surface 112 Second surface 1111 Detection area 1112 Peripheral area 113 First detection electrode layer 1131 First detection electrode 114 Second detection electrode layer 1141 Second detection electrode 115 Primer layer 116 Electrode trace layer 117 1st insulating layer 118 2nd insulating layer 1181 concave groove 1182 microchannel 2 ribonucleic acid detection device 21 control unit 22 circuit board 23 display element 24 processing unit 25 wireless transmission / reception unit 26 power supply unit

Claims (20)

A substrate having a first surface and a second surface on the opposite side thereof,
A plurality of detection electrode layers provided on the first surface, and
At least one primer layer provided on the plurality of detection electrode layers, insulated from each other, and used for reaction with a sample having ribonucleic acid,
A plurality of electrode trace layers provided on the first surface and electrically connected to the plurality of detection electrodes, and a plurality of electrode trace layers.
A ribonucleic acid detection panel, including. A detection region and a peripheral region surrounding the detection region are provided on the first surface of the substrate, and the plurality of detection electrode layers are provided on the first surface of the detection region, and the plurality of electrode trace layers are provided. The ribonucleic acid detection panel according to claim 1, wherein is provided on the first surface of the peripheral region. The plurality of detection electrode layers include a first detection electrode layer and a second detection electrode layer, and the first detection electrode layer is provided on the first surface in the detection region, and the second detection is performed. The ribonucleic acid detection panel according to claim 2, wherein the electrode layer is provided on the first detection electrode layer, and the first insulating layer is provided between the first and second detection electrode layers. 3. The third aspect of the present invention, wherein the plurality of primer layers are arranged on the second detection electrode layer at intervals, and a second insulating layer is provided between the plurality of primer layers and the second detection electrode layer. The ribonucleic acid detection panel according to. A plurality of concave grooves are provided at positions on the second insulating layer corresponding to the plurality of primer layers, and the plurality of primer layers are housed in the plurality of concave grooves and correspond to the respective primer layers. The ribonucleic acid detection panel according to claim 4, wherein a microchannel is defined between the inner wall of each concave groove. The ribonucleic acid detection panel according to claim 1, wherein the substrate is a glass substrate, a circuit board, or a polyethylene terephthalate substrate. The ribonucleic acid detection panel according to claim 1, wherein the at least one primer layer is formed on the plurality of detection electrode layers by a printing or coating method. The material of the plurality of detection electrode layers and the plurality of electrode trace layers is indium tin oxide (ITO), indium zinc oxide (IZO), or antimony-doped tin oxide (ATO), and at least one of the above. The ribonucleic acid detection panel according to claim 1, wherein the material of the primer layer is a polymer material. The ribonucleic acid detection panel according to claim 1, wherein the material of the plurality of detection electrode layers and the plurality of electrode trace layers is a metal material, and the metal material is aluminum, gold, copper, or silver. With the control unit
A substrate having a first surface and a second surface on the opposite side thereof, a plurality of detection electrode layers electrically connected to the control unit and provided on the first surface, and the plurality of detection electrodes. At least one primer layer provided on the layer and used for reaction with a sample having ribonucleic acid and isolated from each other, and the plurality of detection electrode layers provided on the first surface and electrically on the control unit. A ribonucleic acid detection panel, including multiple electrode trace layers connected to,
A ribonucleic acid detector, including. The control unit is electrically connected to a circuit board, and a display element, a processing unit, a wireless transmission / reception unit, and a power supply unit are provided on the circuit board, and the processing unit is the control unit, the display element, and the wireless transmission / reception. The ribonucleic acid detection according to claim 10, which is electrically connected to the unit and is used for supplying power to the display element, the processing unit, the radio transmission / reception unit, and the ribonucleic acid detection panel. Device. The ribonucleic acid detection device according to claim 11, wherein the wireless transmission / reception unit is a Bluetooth (registered trademark) unit, a Wi-Fi unit, or an RF unit. 11. The ribonucleic acid detection device according to claim 11, wherein the display element is a plurality of light emitting diodes or displays, and the control unit is a central processing unit, a microcontroller, or a digital signal processing processor. The ribonucleic acid detection device according to claim 11, wherein the control unit is provided on the first surface of the substrate or on the circuit board. A detection region and a peripheral region surrounding the detection region are provided on the first surface of the substrate, and the plurality of detection electrode layers are provided on the first surface of the detection region, and the plurality of electrode trace layers are provided. 10. The ribonucleic acid detection device according to claim 10, wherein is provided on the first surface of the peripheral region. The plurality of detection electrode layers include a first detection electrode layer and a second detection electrode layer, and the first detection electrode layer is provided on the first surface in the detection region, and the second detection is performed. The ribonucleic acid detection device according to claim 15, wherein the electrode layer is provided on the first detection electrode layer, and the first insulating layer is provided between the first and second detection electrode layers. 16. The 16th aspect of the present invention, wherein the primer layers are arranged on the second detection electrode layer at intervals, and a second insulating layer is provided between the plurality of primer layers and the second detection electrode layer. Ribonucleic acid detector. A plurality of grooves are provided at positions on the second insulating layer corresponding to the plurality of primer layers, and the plurality of primer layers are housed in the plurality of grooves of the second insulating layer. The ribonucleic acid detection device according to claim 17, wherein a microchannel is defined between the primer layer and the corresponding inner wall of each concave groove. The substrate is a glass substrate, a circuit substrate, or a polyethylene terephthalate substrate, the material of the plurality of detection electrode layers and the plurality of electrode trace layers is a metal material, and the metal material is indium tin oxide (ITO). The tenth aspect of claim 10, wherein the material is indium-zinc oxide (IZO), or antimony-doped tin oxide (ATO), aluminum, gold, copper or silver, and the material of the at least one primer layer is a polymer material. Ribonucleic acid detector. The ribonucleic acid detection device according to claim 10, wherein the at least one primer layer is formed on the plurality of detection electrode layers by a printing or coating method.

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