CN111534430B - Ribonucleic acid detection panel and ribonucleic acid detection device - Google Patents
Ribonucleic acid detection panel and ribonucleic acid detection device Download PDFInfo
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- CN111534430B CN111534430B CN202010350040.4A CN202010350040A CN111534430B CN 111534430 B CN111534430 B CN 111534430B CN 202010350040 A CN202010350040 A CN 202010350040A CN 111534430 B CN111534430 B CN 111534430B
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
Abstract
The invention provides a ribonucleic acid detection panel and a ribonucleic acid detection device, wherein the ribonucleic acid detection device comprises a control unit and a ribonucleic acid detection panel, the ribonucleic acid detection panel comprises a substrate, a plurality of induction electrode layers electrically connected with the control unit, at least one primer layer and a plurality of wiring layers, the induction electrode layers are arranged on the first surface of the substrate, the primer layer is arranged on the induction electrode layers and insulated from each other, and the electrode wiring layers are electrically connected with the induction electrodes and the control unit.
Description
Technical Field
The present invention relates to a ribonucleic acid detecting panel and a ribonucleic acid detecting device, and more particularly, to a ribonucleic acid detecting panel and a ribonucleic acid detecting device capable of shortening a detecting time and reducing a cost.
Background
Since it is necessary to send the detected user's symptoms (such as kawasaki disease, colorectal cancer, hand-foot-mouth disease, novel coronavirus (covd-19) or other RNA-bearing viruses) to a measurement platform in a medical institution for marking (such as a Polymerase Chain Reaction (PCR) analyzer), then take the blood of the user, extract the miRNA virus through an RNA extraction instrument, then sequentially add miRNA, add miRNA marking reagent, drop the miRNA marking reagent onto a miRNA wafer for staining and scanning, so as to know whether the user is diagnosed or not according to the change and comparison of fluorescence brightness, so that only specific specialized medical staff will operate the specialized instruments, resulting in the problems of high detection difficulty and high cost. In addition, the conventional procedure for detecting viruses by fluorescent labeling is complicated, so that the detection time is long because it takes at least 2 hours or more than two days to detect the disease.
Disclosure of Invention
One objective of the present invention is to provide a ribonucleic acid detection panel capable of shortening the detection time and reducing the cost.
Another object of the present invention is to provide a ribonucleic acid detecting panel with simple detecting operation and excellent convenience in detecting use.
In order to achieve the above-mentioned objective, the present invention provides a ribonucleic acid detection panel, which comprises a substrate, a plurality of sensing electrode layers, at least one primer layer and a plurality of electrode trace layers, wherein the substrate has a first surface and a second surface opposite to the first surface, the plurality of sensing electrode layers are arranged on the first surface, at least one primer layer is arranged on the plurality of sensing electrode layers and insulated from each other, at least one primer layer is used for reacting with a corresponding detecting body with ribonucleic acid, the plurality of electrode trace layers are arranged on the first surface, and the plurality of electrode trace layers are electrically connected with the plurality of sensing electrodes.
The first surface of the substrate is provided with an induction area and a peripheral area surrounding the induction area, the plurality of induction electrode layers are arranged on the first surface of the induction area, and the plurality of electrode wiring layers are arranged on the first surface of the peripheral area.
The plurality of sensing electrode layers are provided with a first sensing electrode layer and a second sensing electrode layer, the first sensing electrode layer is arranged on the first surface in the sensing area, the second sensing electrode layer is arranged on the first sensing electrode layer, and a first insulating layer is arranged between the first sensing electrode layer and the second sensing electrode layer.
The plurality of primer layers are arranged on the second induction electrode layer at intervals, and a second insulating layer is arranged between the plurality of primer layers and the second induction electrode layer.
The second insulating layer is provided with a plurality of grooves corresponding to the plurality of primer layers, the plurality of primer layers are accommodated in the plurality of grooves, and a micro-channel is defined between each primer layer and the inner wall of each corresponding groove.
The substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate.
At least one primer layer is formed on the plurality of sensing electrode layers by printing or coating.
The material of the plurality of sensing electrode layers and the plurality of electrode wiring layers Is Tin Oxide (ITO), indium Zinc Oxide (IZO) or Antimony Tin Oxide (ATO), and the material of at least one primer layer is a polymer material.
The sensing electrode layers and the electrode wiring layers are made of metal materials, and the metal materials are made of aluminum, gold, copper or silver.
The invention also provides a ribonucleic acid detection device which comprises a control unit and a ribonucleic acid detection panel, wherein the ribonucleic acid detection panel comprises a substrate, a plurality of induction electrode layers, at least one primer layer and a plurality of electrode wiring layers, the substrate is provided with a first surface and a second surface opposite to the first surface, the induction electrode layers are electrically connected with the control unit, the induction electrode layers are arranged on the first surface, at least one primer layer is arranged on the induction electrode layers and insulated from each other, the primer layer is used for reacting with a corresponding detection body with ribonucleic acid, the electrode wiring layers are arranged on the first surface, and the electrode wiring layers are electrically connected with the induction electrode layers and the control unit.
The control unit is electrically connected with the circuit board, the circuit board is provided with a display element, a processing unit, a wireless receiving and transmitting unit and a power supply unit, the processing unit is electrically connected with the control unit, the display element and the wireless receiving and transmitting unit, and the power supply unit is used for providing power for the display element, the processing unit, the wireless receiving and transmitting unit and the ribonucleic acid detection panel.
The wireless transceiver unit is a Bluetooth unit, a Wi-Fi unit and an RF unit.
The display device is a plurality of LEDs or a display, and the control unit is a CPU, a microcontroller or a digital signal processor.
The control unit is arranged on the first surface of the substrate or the circuit board.
The first surface of the substrate is provided with an induction area and a peripheral area surrounding the induction area, the plurality of induction electrode layers are arranged on the first surface of the induction area, and the plurality of electrode wiring layers are arranged on the first surface of the peripheral area.
The plurality of sensing electrode layers are provided with a first sensing electrode layer and a second sensing electrode layer, the first sensing electrode layer is arranged on the first surface in the sensing area, the second sensing electrode layer is arranged on the first sensing electrode layer, and a first insulating layer is arranged between the first sensing electrode layer and the second sensing electrode layer.
The plurality of primer layers are arranged on the second induction electrode layer at intervals, and a second insulating layer is arranged between the plurality of primer layers and the second induction electrode layer.
The second insulating layer is provided with a plurality of grooves corresponding to the plurality of primer layers, the plurality of primer layers are accommodated in the plurality of grooves of the second insulating layer, and a micro-channel is defined between each primer layer and the inner wall of each corresponding groove.
The substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate, the material of the plurality of sensing electrode layers and the plurality of electrode wiring layers is a metal material, the metal material Is Tin Oxide (ITO), indium Zinc Oxide (IZO) or Antimony Tin Oxide (ATO), aluminum, gold, copper or silver material, and the material of at least one primer layer is a polymer material.
At least one primer layer is formed on the plurality of sensing electrode layers by printing or coating.
Therefore, by adopting the design of the invention, the detection time and the cost can be shortened, and the user can automatically and rapidly detect, so that the detection operation is simple and the detection is quite convenient.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description, serve to explain the principles of the invention. In the drawings, like reference numerals are used to identify like elements. The drawings, which are included in the description, illustrate some, but not all embodiments of the invention. Other figures can be derived from these figures by one of ordinary skill in the art without undue effort.
FIG. 1 is an exploded perspective view schematically showing a ribonucleic acid detecting panel according to an embodiment of the present invention.
FIG. 2A is a schematic perspective view illustrating a ribonucleic acid detection panel according to an embodiment of the present invention.
FIG. 2B schematically illustrates a cross-section and a partial enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention.
FIG. 2C is a schematic cross-sectional and partially enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention.
FIG. 3 is an exploded perspective view schematically showing a ribonucleic acid detecting apparatus according to another embodiment of the present invention.
FIG. 4A is a schematic combined perspective view of a ribonucleic acid detecting apparatus according to another embodiment of the present invention.
Fig. 4B schematically illustrates a top view of fig. 4A in another embodiment of the invention.
FIG. 5 is a schematic diagram showing a second embodiment of a RNA detection device according to another embodiment of the present invention.
FIG. 6 is a schematic perspective view showing a third exemplary combination of RNA detection devices according to another embodiment of the present invention.
Description of the reference numerals
Ribonucleic acid detection panel 1
Substrate 11
First surface 111
Second surface 112
Sensing region 1111
Peripheral region 1112
First sensing electrode layer 113
First sense electrode 1131
Second sense electrode layer 114
Second sensing electrode 1141
Primer layer 115
Electrode trace layer 116
First insulating layer 117
Second insulating layer 118
Recess 1181
Micro flow channel 1182
Ribonucleic acid detection device 2
Control unit 21
Circuit board 22
Display element 23
Processing unit 24
Radio transceiver unit 25
Power supply unit 26
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more apparent, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be arbitrarily combined with each other.
The invention provides a ribonucleic acid detection panel and a ribonucleic acid detection device. Referring to fig. 1, fig. 1 is an exploded perspective view schematically showing a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2A is a schematic perspective view illustrating a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2B is a schematic diagram illustrating a cross-section and a partial enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention; FIG. 2C is a schematic cross-sectional and partially enlarged view of a ribonucleic acid detection panel according to an embodiment of the present invention. As shown in fig. 1, 2A and 2B, a Ribonucleic acid (RNA) detection panel 1 includes a substrate 11, a plurality of sensing electrode layers, at least one primer layer 115 and a plurality of electrode trace layers 116, wherein the substrate 11 is a glass substrate 11, a circuit board 22 (such as a flexible circuit board) or a polyethylene terephthalate (PET) substrate 11, the substrate 11 in this embodiment is a glass substrate 11, but not limited thereto, the substrate 11 has a first surface 111 and a second surface 112 opposite to the first surface 111, the first surface 111 of the substrate 11 is provided with a sensing region 1111 and a peripheral region surrounding the sensing region 1111, the plurality of sensing electrode layers 1112 are disposed on the first surface 111 of the sensing region 1111, the plurality of electrode trace layers 116 are disposed on the first surface 111 of the peripheral region 1112, and the plurality of electrode trace layers 116 are electrically connected to the plurality of sensing electrode layers.
The plurality of sensing electrode layers are provided with a transparent or opaque first sensing electrode layer 113 and a transparent or opaque second sensing electrode layer 114, the first sensing electrode layer 113 is provided with a plurality of first sensing electrodes 1131 (such as X-axis sensing electrodes) disposed on the first surface 111 in the sensing region 1111, the second sensing electrode layer 114 is provided with a plurality of second sensing electrodes 1141 (such as Y-axis sensing electrodes) disposed on the first sensing electrode layer 113, and the plurality of first and second sensing electrodes 1131 and 1141 are respectively denoted as X-axis sensing electrodes and Y-axis sensing electrodes in this embodiment and are arranged in an staggered manner. The material of the plurality of sensing electrode layers and the plurality of electrode trace layers 116 is a metal material, such as tin oxide (ITO), indium zinc oxide (Indium zinc oxide, IZO), antimony Tin Oxide (ATO), or a combination thereof, but not limited thereto, and in a specific implementation, the metal material may be aluminum (Al), gold (Au), copper, silver (Ag), or other metal materials (such as nickel (Ni), chromium (Cr), or alloys thereof). The first and second sensing electrodes 1131 and 1141 are diamond-shaped in this embodiment, but not limited thereto, and the first and second sensing electrodes 1131 and 1141 may be strip-shaped, stripe-shaped, wide stripe-shaped or rectangular in one embodiment. In another embodiment, the plurality of first sensing electrodes 1131 are arranged in a matrix array (e.g., an mxn array) on the first surface 111 in the sensing region 1111, the plurality of second sensing electrodes 1141 are arranged in a matrix array (e.g., an mxn array) on the first sensing electrode layer 113, and the plurality of first sensing electrodes 1131 and 1141 are insulated from each other.
In an alternative embodiment, the first and second sensing electrodes 1131 and 1141 of the first and second sensing electrode layers 113 and 114 are disposed on the first surface 111 of the substrate 11 in parallel, and the primer layer 115 is disposed on the first and second sensing electrode layers 113 and 114 and insulated from each other.
A first insulating layer 117 is disposed between the first and second sensing electrode layers 113 and 114, and the first insulating layer 117 is made of silicon dioxide (SiO) 2 ). At least one primer layer 115 is formed on the plurality of sensing electrode layers, for example, by printing or coating, and the primer layer 115 and the plurality of sensing electrode layers are insulated from each other, wherein the primer layer 115 is shown in this embodiment as a plurality of primer layers 115 are disposed on the second sensing electrode layer 114 at intervals, the primer layer 115 is made of a polymer material for reacting with a corresponding detecting body with ribonucleic acid (or micro ribonucleic acid RNA) (such as saliva of a user), for example, when no RNA virus in saliva of the user (i.e. the RNA detecting body) reacts with the primer layer 115 which does not conform to the corresponding specificity, no electrical change (such as no capacitance change or dielectric coefficient change) is formed as if a finger of the user does not touch the touch sensing region 1111, so that a fixed coupling capacitance exists between the plurality of first and second sensing electrodes 1131 and 1141 of the first and second sensing electrode layers 113 and 114, and an electric field (electric lines of force) between the plurality of first and second sensing electrodes 1131 and 1141 is fixed; when the RNA virus in the saliva (i.e. the RNA detecting body) of the user reacts with the primer layer 115 corresponding to the specificity to generate a combination, an obvious electrical change (such as a capacitance change or a dielectric coefficient change) is formed as the finger of the user touches the touch sensing region 1111, so that a capacitance is formed between the primer layer 115 and the second sensing electrode layer 114, and at this time, an electric field (electric line) originally fixedly distributed between the first and second sensing electrodes 1131 and 1141 of the first and second sensing electrode layers 113 and 114 is changed (i.e. the electric field is changed) due to the connection of a part of the electric line to the corresponding primer layer 115, so as to change the capacitance value (i.e. the capacitance value of the coupling capacitance between the first and second sensing electrodes 1131 and 1141).
In addition, the primer layer 115 is a primer that can react with a virus (such as kawasaki disease, colorectal cancer, hand-foot-mouth disease, novel coronavirus (covd-19) or other RNA-bearing virus) to generate binding, for example, the primer with specific specificity is designed in advance to detect the virus (such as novel coronavirus (covd-19), if the RNA-bearing novel coronavirus (covd-19) in saliva (i.e. a detecting body) of the user can react with the primer layer 115 that corresponds to detecting novel coronavirus to generate binding, then the primer has obvious electrical change, and indicates that the user can diagnose (such as positive) to obtain the novel coronavirus (covd-19) (i.e. infected virus); if the saliva (i.e., the test body) of the user is not provided with virus or is provided with other viruses (e.g., kawasaki disease), the primer layer 115 corresponding to the detection of the novel coronavirus does not react with each other and cannot bind, and the electrical property is not changed, the user is not diagnosed (e.g., is negative) that the novel coronavirus is not obtained (i.e., is not infected with virus). Wherein the primer is a small single-stranded DNA or RNA, and is composed of DNA replication origin, DNA replication (RNA primer) occurring in nature and artificially synthesized primer (usually DNA primer) in Polymerase Chain Reaction (PCR).
A second insulating layer 118 is disposed between the plurality of primer layers 115 and the second sensing electrode layer 114, a third insulating layer 119 is disposed between the first sensing electrode layer 113 and the substrate 11, and the second and third insulating layers 118, 119 are made of silicon dioxide (SiO) 2 ) In an alternative embodiment, the third insulating layer 119 may be omitted, so that the first sensing electrode layer 113 is disposed on the first surface 111 of the substrate 11 (e.g., a glass substrate or a PET substrate).
The plurality of primer layers 115 are disposed on the second insulating layer 118, such as elongated, in the sensing region 1111 at intervals along the X-axis direction (or the Y-axis direction), and are disposed at the staggered positions of the plurality of first sensing electrodes 1131 and the plurality of second sensing electrodes 1141. In one embodiment, referring to fig. 2C, a plurality of grooves 1181 are disposed on the second insulating layer 118 corresponding to the plurality of primer layers 115, the plurality of primer layers 115 are accommodated in the plurality of grooves 1181 of the second insulating layer 118, and a micro-channel 1182 is defined between each primer layer 115 and an inner wall of each corresponding groove 1181, wherein the micro-channel 1182 is used for collecting a detecting body (such as saliva), so as to increase a contact area between the detecting body and the corresponding primer layer 115, thereby effectively improving the detection accuracy and shortening the detection time.
Therefore, by the design of the ribonucleic acid detection panel 1, the detection time and the cost can be shortened, and a user can quickly and immediately detect the body condition by self saliva, so that the detection operation is simple and the detection is quite convenient.
Referring to FIG. 3, an exploded perspective view of a ribonucleic acid detecting device according to another embodiment of the present invention is schematically shown; FIG. 4A is a schematic perspective view showing a first embodiment of a ribonucleic acid detecting apparatus according to another embodiment of the present invention; FIG. 4B schematically illustrates the top view of FIG. 4A in another embodiment of the invention; FIG. 5 is a schematic diagram schematically showing a second embodiment of a RNA detection device according to another embodiment of the present invention; FIG. 6 is a schematic perspective view of a third exemplary combination of RNA detection devices according to another embodiment of the present invention, with reference to FIG. 1. In this embodiment, the Ribonucleic acid (RNA) detection panel 1 of the first embodiment is mainly applied to a Ribonucleic acid (Ribonucleic acid) detection device 2, that is, the Ribonucleic acid detection device 2 of this embodiment includes a control unit 21 and the Ribonucleic acid detection panel 1, and the structure and the connection relationship and the efficacy of the Ribonucleic acid (RNA) detection panel 1 are the same as those of the Ribonucleic acid (RNA) detection panel 1 of the first embodiment, so that the description thereof will not be repeated here. The control unit 21 is a Central Processing Unit (CPU), a Micro Controller Unit (MCU) or a Digital Signal Processor (DSP), and the control unit 21 is electrically connected to the electrode trace layers 116 and the sensing electrode layers, and the control unit 21 determines whether the detecting body and the primer layer 115 have electrical changes according to the signals of capacitance changes transmitted by the sensing electrode layers (i.e. the first sensing electrodes 1131 and the second sensing electrodes 1141) and generates a detection result, where the detection result is a result of electrical changes (e.g. capacitance changes) between the detecting body and the primer layer 115 or a result of no electrical changes (e.g. no capacitance changes) between the detecting body and the primer layer 115.
The control unit 21 in the present embodiment is configured in three ways as follows: in the first aspect, as shown in fig. 3, 4A, and 4B, the control unit 21 is disposed on the first surface 111 of the peripheral area 1112 of the substrate 11 (e.g. the glass substrate 11 or the PET substrate 11), and the control unit 21 is combined with the first surface 111 of the substrate 11 by a chip-on-glass (COG) method, the control unit 21 is electrically connected to the circuit board 22 (e.g. the flexible circuit board 22 and the fpc), the circuit board 22 is integrally combined with the substrate 11 by a thermal compression method, the circuit board 22 is provided with the display element 23, the processing unit 24, the wireless transceiver unit 25 and the power supply unit 26, the processing unit 24 is electrically connected to the control unit 21, the display element 23 and the wireless transceiver unit 25, the processing unit 24 is a Central Processing Unit (CPU), a Digital Signal Processor (DSP) or a controller (MCU), for processing and executing the signals, e.g. the processing unit 24 processes according to the electrical change of the detection result transmitted by the control unit 21, and generates the detection result information, the display element 23 displays the detection result information (e.g. the positive display element 23) and the non-diagnostic result is displayed by the user if the detection result transmitted by the control unit 21 is not displayed by the control unit or the user has the diagnostic result displayed by the user that the user has the diagnostic result displayed by the user has not displayed by the diagnostic result.
The display element 23 is shown as a display for displaying the information of the detection result in this embodiment, but not limited thereto, and in other embodiments, the display element 23 may be a plurality of Light Emitting Diodes (LEDs), that is, a plurality of lamps are used to indicate the detection result, for example, a bright red LED lamp is used to indicate positive diagnosis, and a bright blue LED lamp is used to indicate negative diagnosis. The power supply unit 26 is a battery in this embodiment, and the power supply unit 26 is used for providing power to the display element 23, the processing unit 24, the wireless transceiver unit 25 and the ribonucleic acid detection panel 1. In one embodiment, the power unit 26 may be a rechargeable battery, and the circuit board 22 is provided with a connection port (e.g. Micro USB connection port) for charging the power unit 26.
The wireless transceiver unit 25 is a bluetooth unit (e.g. bluetooth transceiver) in this embodiment, and the bluetooth unit is configured to be wirelessly connected to an electronic device (e.g. smart phone, smart watch, computer, pen or tablet; not shown), so that the processing unit 24 can wirelessly transmit the detection result information to the electronic device for display. In particular implementations, the wireless transceiver unit 25 is a Wi-Fi unit or an RF (radio frequency) unit.
When a user wants to detect whether kawasaki disease (or mucosal cutaneous lymphadenopathy (Mucocutaneous Lymph Node Syndrome)) virus, the user can drop his saliva (i.e. the detection body) onto the sensing area 1111, so that the miRNA with miRNA in the saliva is selected from miR-30e-3P and is combined with the primer layer 115 corresponding to the detection of kawasaki disease virus to generate obvious electrical changes (such as capacitance changes), the control unit 21 determines that the detection body and the primer layer 115 have electrical changes according to the signals of capacitance changes transmitted by the first and second sensing electrodes 1131 and 1141, and generates a detection result to be transmitted to the processing unit 24, so that the processing unit 24 processes the detection result according to the electrical changes, and transmits the detection result information to the display element 23, so that the user can learn (such as table 1) by the detection result information displayed by the display element 23. If the miRNA in the saliva of the user is miR-223-3P (no kawasaki virus with miRNA) and the primer layer 115 corresponding to the detected kawasaki virus cannot be combined, there is no electrical change, so that the processing unit 24 processes the detection result transmitted by the control unit 21 according to the electrical change, and transmits the detection result information, so that the user can know the detection result information displayed by the display element 23 (as shown in table 1). In the graph, the detection result value (e.g. 32.5) corresponding to the detection body miR-30e-3P is larger than the detection result value (e.g. 6.2) corresponding to the detection body miR-223-3P, and the preset reference value of the detection result value is 15, that is, the detection result value is 15 or larger indicates diagnosis, the larger the detection result value indicates the longer the days with viruses in the body of the user, and the more serious the symptoms are, if the detection result value is smaller than 15, the diagnosis is not confirmed.
TABLE 1 detection results Table of the detection body and the corresponding primer layer in another embodiment of the invention
Therefore, the ribonucleic acid detecting device 2 of the present invention is designed to detect the disease by an electrical measurement method, so that the detecting time can be effectively shortened, such as the detection of the disease (e.g. disease virus) within 15 minutes, and the cost can be reduced, and any user can detect the disease in real time at home, thereby not only achieving simple detecting operation, but also being quite convenient in detecting use.
Finally, it should be noted that: in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising one …" does not exclude the presence of other like elements in a process, method, article, or apparatus that comprises the element.
The above embodiments are only for illustrating the technical solution of the present invention, and are not limiting. Although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (14)
1. A ribonucleic acid detection panel, comprising:
a substrate having a first surface and a second surface opposite to the first surface;
a plurality of sensing electrode layers disposed on the first surface;
at least one primer layer arranged on the plurality of induction electrode layers and insulated from each other, wherein the at least one primer layer is used for reacting with a corresponding detection body with ribonucleic acid; a kind of electronic device with high-pressure air-conditioning system
The electrode wiring layers are arranged on the first surface and are electrically connected with the sensing electrode layers;
the plurality of induction electrode layers are provided with a first induction electrode layer and a second induction electrode layer, the first induction electrode layer is arranged on the first surface, the second induction electrode layer is arranged on the first induction electrode layer, and a first insulating layer is arranged between the first induction electrode layer and the second induction electrode layer; the first induction electrode layer is provided with a plurality of first induction electrodes, the second induction electrode layer is provided with a plurality of second induction electrodes, and the plurality of first induction electrodes and the plurality of second induction electrodes are arranged in a staggered manner; the plurality of primer layers are arranged on the second induction electrode layer corresponding to the staggered positions of the plurality of first induction electrodes and the plurality of second induction electrodes at intervals, a second insulating layer is arranged between the plurality of primer layers and the second induction electrode layer, a plurality of grooves are formed in the second insulating layer corresponding to the plurality of primer layers, the plurality of primer layers are accommodated in the plurality of grooves, and a micro-channel is defined between each primer layer and the inner wall of each corresponding groove.
2. The ribonucleic acid detection panel according to claim 1, wherein the first surface of the substrate is provided with a sensing region and a peripheral region surrounding the sensing region, the plurality of sensing electrode layers are disposed on the first surface of the sensing region, and the plurality of electrode trace layers are disposed on the first surface of the peripheral region.
3. The ribonucleic acid detection panel according to claim 1, wherein the substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate.
4. The ribonucleic acid detection panel according to claim 1, wherein the at least one primer layer is formed on the plurality of sensing electrode layers by printing or plating.
5. The ribonucleic acid detecting panel according to claim 1, wherein the material of the plurality of sensing electrode layers and the plurality of electrode trace layers Is Tin Oxide (ITO), indium Zinc Oxide (IZO), antimony Tin Oxide (ATO), and the material of the at least one primer layer is a polymer material.
6. The ribonucleic acid detection panel according to claim 1, wherein the material of the plurality of sensing electrode layers and the plurality of electrode trace layers is a metal material, and the metal material is an aluminum, gold, copper or silver material.
7. A ribonucleic acid detecting apparatus, comprising:
a control unit; and
a ribonucleic acid detection panel comprising:
a substrate having a first surface and a second surface opposite to the first surface;
the plurality of sensing electrode layers are electrically connected with the control unit and are arranged on the first surface;
at least one primer layer arranged on the plurality of induction electrode layers and insulated from each other, wherein the at least one primer layer is used for reacting with a corresponding detection body with ribonucleic acid; a kind of electronic device with high-pressure air-conditioning system
The electrode wiring layers are arranged on the first surface and are electrically connected with the sensing electrode layers and the control unit;
the plurality of induction electrode layers are provided with a first induction electrode layer and a second induction electrode layer, the first induction electrode layer is arranged on the first surface, the second induction electrode layer is arranged on the first induction electrode layer, and a first insulating layer is arranged between the first induction electrode layer and the second induction electrode layer; the first induction electrode layer is provided with a plurality of first induction electrodes, the second induction electrode layer is provided with a plurality of second induction electrodes, and the plurality of first induction electrodes and the plurality of second induction electrodes are arranged in a staggered manner; the plurality of primer layers are arranged on the second induction electrode layer corresponding to the staggered positions of the plurality of first induction electrodes and the plurality of second induction electrodes at intervals, a second insulating layer is arranged between the plurality of primer layers and the second induction electrode layer, a plurality of grooves are formed in the second insulating layer corresponding to the plurality of primer layers, the plurality of primer layers are accommodated in the plurality of grooves, and a micro-channel is defined between each primer layer and the inner wall of each corresponding groove.
8. The ribonucleic acid detecting apparatus according to claim 7, wherein the control unit is electrically connected to a circuit board, and the circuit board is provided with a display element, a processing unit, a wireless transceiver unit and a power supply unit, and the processing unit is electrically connected to the control unit, the display element and the wireless transceiver unit, and the power supply unit is configured to supply power to the display element, the processing unit, the wireless transceiver unit and the ribonucleic acid detecting panel.
9. The ribonucleic acid detecting apparatus according to claim 8, wherein the radio transceiver unit is a bluetooth unit, a Wi-Fi unit, and an RF unit.
10. The ribonucleic acid detecting apparatus according to claim 8, wherein the display element is a plurality of light emitting diodes or a display, and the control unit is a central processing unit, a microcontroller or a digital signal processor.
11. The ribonucleic acid detection device according to claim 8, wherein the control unit is provided on the first surface of the substrate or on the circuit board.
12. The ribonucleic acid detection device according to claim 7, wherein the first surface of the substrate is provided with an induction zone and a peripheral zone surrounding the induction zone, the plurality of induction electrode layers are arranged on the first surface of the induction zone, and the plurality of electrode trace layers are arranged on the first surface of the peripheral zone.
13. The ribonucleic acid detecting apparatus according to claim 7, wherein the substrate is a glass substrate, a circuit board or a polyethylene terephthalate substrate, the material of the plurality of sensing electrode layers and the plurality of electrode trace layers is a metal material, the metal material Is Tin Oxide (ITO), indium Zinc Oxide (IZO) or Antimony Tin Oxide (ATO), aluminum, gold, copper or silver material, and the material of the at least one primer layer is a polymer material.
14. The ribonucleic acid detecting apparatus according to claim 7, wherein the at least one primer layer is formed on the plurality of sensing electrode layers by printing or plating.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101592627A (en) * | 2009-03-19 | 2009-12-02 | 苏州纳米技术与纳米仿生研究所 | The making integrated approach of multichannel high-sensitive biosensor |
| TWM462368U (en) * | 2013-02-04 | 2013-09-21 | Wen Wang | Electrochemical biosensor without hematocrit correction |
| TW202141033A (en) * | 2020-04-20 | 2021-11-01 | 關鍵禾芯科技股份有限公司 | Ribonucleic acid test panel and ribonucleic acid test device |
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| TWI383144B (en) * | 2008-09-23 | 2013-01-21 | Univ Nat Chiao Tung | Sensing element, manufacturing method and detecting system thereof |
| US20110312567A1 (en) * | 2010-06-17 | 2011-12-22 | Geneasys Pty Ltd | Loc device for electrochemiluminescent detection of target nucleic acid sequences using hybridization chamber array and negative control chamber without probes |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101592627A (en) * | 2009-03-19 | 2009-12-02 | 苏州纳米技术与纳米仿生研究所 | The making integrated approach of multichannel high-sensitive biosensor |
| TWM462368U (en) * | 2013-02-04 | 2013-09-21 | Wen Wang | Electrochemical biosensor without hematocrit correction |
| TW202141033A (en) * | 2020-04-20 | 2021-11-01 | 關鍵禾芯科技股份有限公司 | Ribonucleic acid test panel and ribonucleic acid test device |
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