JP2021155419A - Composition for lipase activity inhibition - Google Patents

Abstract

To reveal the health benefits of Ecklonia kurome and to provide compositions that can be used in foods and the like.SOLUTION: Provided is a composition for lipase activity inhibition, lipid absorption inhibition, stool slippage improvement, or intestinal regulation, which contains an effective amount of the water extract of Ecklonia kurome (scientific name: Ecklonia kurome). Provided is a method for producing the water extract or processed product of Ecklonia kurome, comprising the following step: (1) extracting Ecklonia kurome with hot water at 50 to 80°C for 1 to 5 hours.SELECTED DRAWING: Figure 1

Description

Ecklonia cava (scientific name: Ecklonia cava) is a type of brown alga of the genus Ecklonia cava in the family Ecklonia cava. It has been confirmed that it is distributed on Ecklonia cava, etc. The adult size is usually 20-50 cm.

Ecklonia cava and Ecklonia cava are known as species with similar morphology, but Ecklonia cava is distinguished from Ecklonia cava in that it does not wrinkle in the leaves and arame is bifurcated at the upper part of the stem.

Ecklonia kurome is mainly eaten by algae harvested in winter, and in some areas such as Saganoseki in Oita prefecture, as a special product, in addition to chopped raw kurome, seasonings (soy sauce, sauce, etc.) and processing It is used in food. Chrome is rich in amino acids and minerals and is expected to have health benefits.

However, looking at Japan as a whole, the chrome obtained from the harvest of other marine products is discarded, and it cannot be said that it is a fully utilized foodstuff. In response to this situation, the development of aquaculture technology is being promoted due to the high value of Ecklonia kurome as an edible product (Non-Patent Documents 1 and 2).

Tomokazu Nishigaki et al., "Distribution characteristics and seasonal fate of brown algae kurome in the western waters of Wakasa Bay," Kyoto Prefectural Agriculture, Forestry and Fisheries Technology Center Marine Center Research Report, No. 37, 2015, P.M. 1-6 Tomokazu Nishigaki et al., "Growth and Morphology of Cultured Ecklonia kurome in Kamanyu, Kyoto Prefecture," Kyoto Prefectural Marine Center Research Report, No. 28, 2006, P.M. 16-20 Hiroaki Ueno et al., "Trends and Prospects of New Anti-Obesity Drugs", Journal of the Japanese Society of Internal Medicine, Vol. 103, No. 3, 2014, P.M. 753-759

As mentioned above, Ecklonia kurome is a seaweed that is often discarded, and has not yet been fully utilized in foods, etc., and there are few detailed studies or research reports on the health effects of Ecklonia kurome.

Therefore, an object of the present invention is to clarify the health effect of Ecklonia kurome and to provide a composition that can be used in foods and the like.

As a result of diligent studies by the present inventors in order to solve the above problems, it was found that the water extract of Ecklonia kurome has a remarkable inhibition of lipase activity, and the present invention has been completed.

That is, the present invention provides the compositions listed below.
[1]
A composition for inhibiting lipase activity, which contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome).

[2]
A composition for suppressing lipid absorption, improving stool slippage, or intestinal regulation, which contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome).

[3]
The composition according to [1] or [2], wherein the Ecklonia cava genus Ecklonia cava is a dried product of Ecklonia cava.

[4]
The composition according to any one of [1] to [3], wherein the extraction site of Ecklonia cava is a frond and / or a stem.

[5]
The composition according to any one of [1] to [4], wherein the Ecklonia cava has a growth period of 1 to 6 years.

[6]
The composition according to any one of [1] to [5], wherein the extraction temperature for obtaining the extract is 50 to 80 ° C.

[7]
The composition according to any one of [1] to [6], wherein the extraction time for obtaining the extract is 1 to 5 hours.

[8]
The composition according to any one of [1] to [7], which is a drug, a quasi drug, a cosmetic product, or a food or drink.

[9]
The composition according to any one of [1] to [8], wherein the daily intake is 500 mg or more in terms of dry solid content.

The present invention also provides the following manufacturing methods.
[10]
The following steps:
(1) A method for producing an aqueous extract or processed product of Ecklonia cava, which comprises a step of extracting Ecklonia cava with hot water at 50 to 80 ° C. for 1 to 5 hours.

[11]
The method according to [10], which is a water extract or processed product of Ecklonia cava, which has an enhanced lipid absorption inhibitory effect.

The present invention also provides the following processed products of the genus Ecklonia cava.

[12]
Contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome),
The extraction temperature for obtaining the extract is 50 to 80 ° C.
The extraction time to obtain the extract is 1-5 hours.
Processed product of Ecklonia cava.

According to the present invention, it is possible to provide a composition for inhibiting lipase activity, suppressing lipid absorption, improving stool slippage, or intestinal regulation by using a water extract of Ecklonia kurome that can be used in foods and the like. ..

FIG. 1 is a graph showing the results of a lipase activity inhibition test of a water extract (test sample) of Ecklonia kurome obtained from each temperature and extraction time in Test Example 2.

[Composition for Inhibiting Lipase Activity]
In the present invention, the composition for inhibiting lipase activity contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome).

Ecklonia kurome is a species of brown alga of the genus Ecklonia cava in the family Ecklonia cava. In the present invention, the applicable chrome site is not limited as long as the effect of the present invention is exhibited, but at least one selected from the group consisting of, for example, fronds, stems (core), and buds. The fronds and / or stems are preferred.

Kurome is widely distributed in the waters near Japan from the central and southern part of the Pacific coast of Honshu to Kyushu, the Seto Inland Sea, and the coast of Japan, and overseas, it has been confirmed that it is distributed on Jeju Island in South Korea. In general, the growth of seaweed and the amount of nutritional components of seaweed differ depending on the strength of ocean currents, water temperature, height of the seabed, and the like. The production area of Kurome is not limited as long as the effect of the present invention is exhibited, but examples thereof include Tottori prefecture, Oita prefecture, Kumamoto prefecture, Ehime prefecture, Aichi prefecture, etc., Tottori prefecture, Oita prefecture, etc. It is preferably produced in Kumamoto prefecture or Kumamoto prefecture, more preferably produced in Tottori prefecture or Oita prefecture, and particularly preferably produced in Tottori prefecture. Here, the products produced in each prefecture are those that can be harvested in either the sea area (port area, fishing port area, general sea area) or the coast (coastal conservation area, general public coast area) managed by the prefecture. Although not limited, the time for collecting Ecklonia kurome is preferably February to August, and more preferably April to June, for example, in the case of Ecklonia kurome produced in Tottori Prefecture, from the viewpoint of remarkably exerting the effect of the present invention.

Although Kurome is a perennial plant, its growing period is not limited as long as the effects of the present invention are exhibited. The growing period of Ecklonia kurome is, for example, preferably 1 to 6 years, more preferably 1 to 5 years, further preferably 1 to 4 years, particularly preferably 1 to 3 years, and most preferably 1 to 2 years. preferable. Ecklonia kurome grows in the first year, and the morphology is bamboo leaf-like. It is possible to discriminate the chrome of the eyes and the like.

The water extract of Ecklonia kurome is prepared by extracting with water by a conventional method as long as the effect of the present invention is exhibited, and may be an extract or a dried product thereof.

Although not limited, for example, a water extract of chrome is obtained by immersing the chrome in a raw state, a dried product, or a pulverized product in water and immersing and extracting the chrome under optimum temperature conditions and time conditions. It is possible to prepare. Although not limited, in Examples (Test Example 6) described later, it has been confirmed that the use of a dried product of Ecklonia kurome exerts a higher effect of the present invention. The method for obtaining the dried product of Ecklonia kurome is not particularly limited, and a known method can be used. Examples thereof include sun-drying and obtaining a dried product of Ecklonia kurome by a known dryer. When sun-drying, the conditions vary depending on the weather, season, time of day, etc. and can be adjusted as appropriate, but it is recommended to sun-dry at least 6 hours or more, 12 hours or more, 24 hours (1 day) or more, etc. Be done. When a dryer is used, the drying conditions are not particularly limited, and examples thereof include freeze-drying and heat-drying. The heating temperature for heat drying is, for example, preferably 30 to 50 ° C, more preferably 35 to 40 ° C. The heating time for heat drying is, for example, preferably 1 to 24 hours, more preferably 1 to 10 hours, and even more preferably 1 to 5 hours.

In another embodiment, for example, chrome is squeezed to obtain squeezed juice, the squeezed lees separated by squeezing is immersed in water, and the juice is extracted by immersion under optimum temperature and time conditions. And, after obtaining the extract, each may be mixed and used as a water extract of Kurome.

The temperature conditions in the immersion extraction are not limited as long as the effects of the present invention are exhibited, but may be, for example, room temperature or heating. When performing immersion extraction by heating, the extraction temperature is, for example, preferably 30 to 120 ° C, more preferably 40 to 110 ° C, further preferably 50 to 90 ° C, even more preferably 50 to 80 ° C, and 60 to 80 ° C. Is even more preferable, 60 to 75 ° C. is even more preferable, 65 to 75 ° C. is even more preferable, and 65 to 70 ° C. (for example, about 70 ° C.) is most preferable.

The time conditions in the immersion extraction are not limited as long as the effects of the present invention are exhibited, but the extraction time is preferably, for example, 10 minutes to 24 hours, more preferably 30 minutes to 10 hours, and 1 hour to 5 hours (1 hour to 5 hours). For example, 1 hour to 3 hours is more preferable, and 2 hours to 4 hours (for example, 2.5 hours to 3.5 hours, 3 hours) is particularly preferable.

[Use]
The composition for inhibiting lipase activity of the present invention has at least the effect of inhibiting the activity of a lipolytic enzyme (lipase) in vivo. The lipase activity inhibitory effect can be evaluated by the method described in Examples described later.

The composition for inhibiting lipase activity of the present invention is used for suppressing lipid absorption, suppressing visceral fat accumulation, improving obesity, etc. by exerting the effect of inhibiting the activity of lipolytic enzyme (lipase) in vivo. In addition, it can be suitably used for intestinal regulation, improvement of intestinal bacterial industry, improvement of intestinal environment, and the like.

In addition, since the present invention suppresses lipid decomposition and promotes the excretion of lipids from the body, it is suitably used for increasing the amount of lipids in stool, promoting the effect of promoting defecation, and improving the slippage of stool during defecation. be able to. This is clear from the reports that pharmaceutical preparations with a mechanism of action that inhibits lipase activity are used as anti-obesity drugs by suppressing the absorption of triglycerides, and are accompanied by an increase in the number of defecations and an increase in oily stools (non-). Patent Document 3).

In addition, the present invention can be suitably used for improving oily skin because lipid decomposition is suppressed and lipid excretion to the outside of the body can be promoted.

The composition of the present invention can be prepared and processed as a pharmaceutical product, a quasi drug, a cosmetic product, a food or drink, a feed, or a pet food, without limitation.

For example, when prepared as a food or drink, a food or drink that displays as functionality such as suppression of lipid absorption, suppression of visceral fat accumulation, improvement of obesity, intestinal regulation, improvement of intestinal bacterial industry, improvement of intestinal environment, improvement of stool slippage, that is, , Health foods, foods with functional claims, foods for the sick, foods for specified health use, etc.

Specifically, health foods, foods with functional claims, foods for the sick, and foods for specified health use are solid preparations (tablets, granules, fine granules, powders, capsules, chewable tablets, etc.) and liquids (syrups). , Sustainable agent), liquid food and the like. The food product in the form of a preparation can be produced in the same manner as a known pharmaceutical preparation, and is produced by mixing the active ingredient with a carrier acceptable as a food product, for example, a suitable excipient, and the like, and then using conventional means. be able to.

For example, tablets can be prepared by mixing powdered active ingredients and pharmaceutically acceptable carrier ingredients (excipients, etc.) and compression molding, and confectionery tablets such as candies can be molded into molds. It may be prepared by the method of injection. The tablets may be sugar-coated or film-coated. Further, the tablet may be a single-layer tablet or a laminated tablet such as a two-layer tablet.

Granules such as granules are available in various granulation methods (extrusion granulation method, pulverized granulation method, dry compaction granulation method, fluidized layer granulation method, rolling granulation method, high-speed stirring granulation method, etc.). The tablets can be prepared by appropriately combining the above-mentioned granulation method, tableting method (wet tableting method, direct tableting method) and the like.

Capsules can be prepared by filling capsules (soft or hard capsules) with powders (powder, granules, etc.), liquids, or the like by a conventional method.

The liquid preparation can be prepared by dissolving or dispersing each component in an aqueous medium (purified water, ethanol-containing purified water, etc.) as a carrier component, filtering or sterilizing if necessary, filling in a predetermined container, and sterilizing. ..

Soft capsules have a smooth surface and are easy to swallow, and are preferred by users. Examples of a general method for producing a soft capsule include a flat plate method, a rotary method, and a seamless method.

In the production of the rotary method (punching method), a sheet-shaped capsule film sandwiches a flowing filling content and forms a capsule shape along a hole of a rotating cylindrical die. On the other hand, in the seamless method (dropping method), the capsule film composition and the contents are simultaneously discharged from the concentric multiple nozzles to form a seamless capsule shape.

The base of the film of the soft capsule is not particularly limited, but starch, pullulan, cellulose, polyvinyl alcohol, gelatin, saccharified gelatin and the like can be used, and starch, gelatin and saccharified gelatin are preferable, and gelatin and succinic are preferable. Gelatinized gelatin is more preferred. These may be used alone or in combination of two or more.

The preferred dosage form of the solid preparation of the present invention is a capsule or a tablet, and more preferably a soft capsule (soft capsule, soft capsule).

In addition, soups, juices, fruit juice drinks, milk, milk drinks, milk drinks, lactic acid bacteria drinks, tea drinks, alcoholic drinks, coffee drinks, carbonated drinks, soft drinks, water drinks, cocoa drinks, jelly-like drinks, sports The composition of the present invention can be produced as a beverage, a liquid beverage such as a diet beverage, a semi-solid food such as pudding and yogurt, noodles, confectionery, spreads and the like.

Examples of soups include miso soup, kurome soup, and kurome chazuke. Although not limited, a useful component in Ecklonia kurome is obtained by immersing the algae of Ecklonia kurome (which may be a dried product of the algae) in water and heat-treating it under the conditions of 50 to 80 ° C. for 1 to 5 hours. It becomes possible to produce ingredients for soups containing this extract. It is also possible to use a dried product of Ecklonia kurome algae or a crushed product thereof as a soup stock, and use it for various Japanese foods such as soup stock. It is also possible to add a dried product of Ecklonia kurome algae or a crushed product thereof to salty sauce or the like and use it as a seasoning. By performing the heat treatment within the range of the above conditions, it is expected that a component effective for the lipase activity inhibitory effect is eluted and the component is efficiently ingested.

In addition, the composition of the present invention can be produced as boiled foods. Boiled foods are dishes that use chrome as one of the raw materials (ingredients), boiled with water, soup stock, etc., and seasoned with seasonings such as shio, sake, sugar, and mirin. Examples of the simmered foods include kurome tsukudani, kurome simmered, simmered with kurome, simmered kurome, simmered kurome, and stewed. Among these simmered foods, from the viewpoint of performing heat treatment for a long time, tsukudani, simmered, simmered or boiled kurome is preferable, and tsukudani or simmered kurome is more preferable.

Although not limited, the algae of Ecklonia kurome (which may be a dried algae) is soaked in water and heat-treated with soup stock and seasonings for 1 to 5 hours under the above-mentioned conditions such as 50 to 80 ° C. By doing so, it becomes possible to extract useful components in kurome and produce simmered foods containing this extract. By performing the heat treatment within the range of the above conditions, it is expected that a component effective for the lipase activity inhibitory effect is eluted and the component is efficiently ingested. In addition, in boiling or boiling, it may be boiled until the water (juice) disappears, but by performing the heat treatment within the above conditions, the components effective for the lipase activity inhibitory effect are once eluted. , At the stage of boiling or lowering the temperature after boiling, the above active ingredient soaks into chrome and other ingredients, leading to efficient intake of the ingredient.

The above soups and simmered foods can also be sold as processed products for prepared foods and prepared meals. Conventional processed products using chrome have been cooked under high heat (90 to 100 ° C., etc.) and short time (within 10 minutes, etc.) from the viewpoint of shortening cooking time and production efficiency. In the present invention, it is possible to cook a processed product of Ecklonia cava, which has significantly enhanced lipase activity inhibitory effect and health function, as it is cooked under low heat and long-term conditions, which are not used under conventional cooking conditions. Become.

When the composition of the present invention is prepared as a food or drink, various food additives may be blended. Examples of food additives include antioxidants, pigments, flavors, seasonings, sweeteners, acidulants, pH adjusters, quality stabilizers, preservatives and the like.

When the composition of the present invention is prepared as a health food, a food with functional claims, a food for the sick, and a food for specified health use, other fat absorption inhibitory components may be blended. For example, indigestible dextrin, isomaltodextrin, EPA, DHA, gallic acid, terminaria berylica-derived gallic acid, seitakamilovalan fruit-derived gallic acid, globin-derived valine-valine-tyrosine-proline, African mangonoki-derived ellagic acid, Examples include, but are not limited to, inulin, gymnemic acid, and the like.

When the composition of the present invention is prepared as a pharmaceutical product or a quasi-drug, it can be prepared as a preparation containing an aqueous extract of Ecklonia kurome, which can be an active ingredient, and preferably a pharmaceutically acceptable carrier. A pharmaceutically acceptable carrier generally refers to an inert, non-toxic, solid or liquid bulking agent, diluent, encapsulating material, etc. that does not react with the active ingredient, such as water. , Ethanol, polyols, suitable mixtures thereof, solvents such as vegetable oils or dispersion media and the like.

Pharmaceuticals and quasi-drugs are administered orally, parenterally, for example, in the oral cavity, in the digestive tract, or in the nasal cavity. Examples of the orally administered preparation include solid preparations (tablets, granules, fine granules, powders, capsules, chewable tablets, etc.) and liquid preparations (syrups, suspensions, inhalants) and the like. Examples of the parenteral administration preparation include infusions, nasal drops, injections and the like.

Pharmaceuticals and quasi-drugs may further contain additives commonly used in the pharmaceutical field. Such additives include, for example, excipients, binders, disintegrants, lubricants, antioxidants, colorants, flavoring agents and the like, which can be used as appropriate as needed. Since it is sustained-release so that it can act for a long time, it can be coated with a known retarder or the like. For pharmaceutical products and quasi-drugs, other additives and drugs such as antacids and gastric mucosa protective agents may be added as needed.

Pharmaceuticals and quasi-drugs can be applied in the form of oral compositions, oral compositions and the like. In addition, pharmaceuticals and quasi-drugs may be used therapeutically or non-therapeutically.

In addition, when used in the form of pharmaceuticals, quasi-drugs, cosmetics, etc., fatty acids are produced from sebum by lipases produced by microorganisms in the skin, and the generated fatty acids activate neutrophils and are activated. Neutrophils release active oxygen, which can contribute to rough skin. By blending the water extract of Ecklonia kurome in the form of an external preparation or the like, it is possible to provide a composition for inhibiting lipase activity that is effective against rough skin such as acne.

The daily oral intake or dose of Ecklonia kurome's water extract for adults is the individual's condition, body weight, gender, age, material activity, intake or route of administration, intake or administration schedule, formulation form or other factors. Can be appropriately determined. The oral intake or dose of the water extract of Ecklonia kurome per adult per day is, for example, preferably 500 mg / day or more, more preferably 800 mg / day or more, and further 1000 mg / day or more in terms of dry solid content. Preferably, 1500 mg / day or more is particularly preferable, and 2000 mg / day or more is most preferable.

The oral intake or dose of the water extract of Ecklonia kurome per adult per day is, for example, preferably 45 g / day or less, more preferably 40 g / day or less, further preferably 35 g / day or less, and 30 g / day or less. It is particularly preferable, and 25 g / day or less is most preferable.

The oral intake or dose of the water extract of Ecklonia kurome per adult per day is, for example, preferably 500 mg to 45 g / day, more preferably 800 mg to 40 g / day, further preferably 1000 mg to 35 g / day, and 1500 mg to 1500 mg. 30 g / day is particularly preferable, and 2000 mg to 25 g / day is most preferable.

The content of the water extract of Ecklonia kurome can be an amount that is the above-mentioned intake amount or dose. The oral intake or dose per day for adults should be adjusted to 1 to 6 capsules, 1 to 4 capsules, 1 to 3 capsules, or 1 to 2 capsules, for example, in the case of capsules, according to the dosage form. You may take it separately.

The composition of the present invention is divided into 1 to several times a day, and is usually ingested or administered 1 to 6 times a day, 1 to 3 times a day, 1 to 2 times a day, or at arbitrary periods and intervals. obtain.

[Manufacturing method of water extract or processed product of Ecklonia cava]
In the present invention, the method for producing an aqueous extract or processed product of Ecklonia cava includes (1) a step of extracting Ecklonia cava with hot water at 50 to 80 ° C. for 1 to 5 hours.

The above steps can be appropriately set according to the description of the temperature conditions, time conditions, etc. described in the composition for inhibiting lipase activity.

Next, the present invention will be specifically described with reference to Examples, but the present invention is not limited to the following Examples. Further, the amount of the extract shown in the examples is a dry solid content equivalent amount unless otherwise specified.

[Test Example 1: Examination of extraction conditions for water extract of Ecklonia kurome]
Small pieces of 5 g each are prepared from the algae of Ecklonia kurome (scientific name: Ecklonia kurome) (produced in Tottori prefecture, harvest time: April, site: leaf body and stem, growing years: 2 to 5 years), and various temperatures. And an extraction time of 1 hour or 3 hours were combined to prepare an aqueous extract as a test sample, and the extraction conditions using the amount and viscosity of the extract as an index were examined. Table 1 shows the results regarding the amount of extract.

As shown in Table 1, in the water extract of Kurome, temperature-dependent and extraction time-dependent results were obtained, the amount of the extract increased as the temperature increased, and the extraction time was longer at each extraction temperature. It was confirmed that the amount of extract obtained was large.

The results regarding viscosity are shown in Table 2. For the evaluation of viscosity, when 30 mL of each test sample was filtered by a filter (mesh size: 3 μm, manufactured by ADVANTEC), it took 15 minutes or more to complete the filtration, and the filter was replaced every time the sample was clogged. Those using more than one sheet were evaluated as "viscous: x".

As shown in Table 2, the water extract of Ecklonia kurome at 50 ° C. (1 hour treatment and 3 hour treatment), 60 ° C. (1 hour treatment and 3 hour treatment), and 70 ° C. (1 hour treatment) is viscous. Therefore, it took time to operate by filtering. On the other hand, in the water extract of Ecklonia kurome under the condition of 70 ° C. for 3 hours, which was higher than the condition of 3 hours, no obvious viscosity was observed at the time of filter filtration, and the preparation was relatively easy. From the viewpoint of preparation of the extract and convenience as a raw material, it was confirmed that the condition on the higher temperature side than 70 ° C. (3 hour treatment) is desirable.

[Test Example 2: Examination of lipase activity inhibitory effect by extraction conditions]
A lipase activity inhibition test was conducted using an aqueous extract of Ecklonia kurome (test sample) obtained from each extraction temperature and extraction time. In the lipase activity inhibition test, oleic acid ester (4-MUO) of 4-methylumbelliferone was used as a substrate. To 0.05 ml of 4-MUO solution ( ^10-4 mM), 0.1 ml of the test sample was added and mixed, then 0.05 ml of 3 U / mL pancreatic lipase solution was added, and the mixture was reacted at 37 ° C. for 20 minutes. After that, 0.1 ml of hydrochloric acid was added to stop the reaction. The fluorescence of 4-methylumbelliferone (excitation wavelength 320 nm, fluorescence wavelength 450 nm) generated by the reaction in a mixed solution of the reaction solution and the citrate solution was measured with a fluorescence microplate reader. As the activity value, the activity value (IC50) of each test sample was calculated with the value without addition of the test sample as 100%. The lower the activity value, the stronger the lipase inhibitory effect. The results are shown in FIG. In FIG. 1, the broken line shows the result at the extraction time of 1 hour, and the solid line shows the result at the extraction time of 3 hours.

As shown in FIG. 1, the water extract of Ecklonia kurome prepared at 70 ° C. for 3 hours showed the most remarkable inhibition of lipase activity. Interestingly, it was found that the degree of lipase activity inhibition decreased as the temperature conditions were shifted from 70 ° C. to the low temperature side and the high temperature side.

It is expected that the components present in the solution are different between the viscous solution on the low temperature side and the solution on the high temperature side, but similar results were obtained as a result. From this, it was suggested that the ratio of each chrome component was extracted in a well-balanced manner under the extraction conditions at 70 ° C. From the simplicity of the preparation method and the results of the evaluation of lipase activity inhibition, it was judged that extraction at 70 ° C. for 3 hours was optimal. In addition, even in the extraction at 50 ° C. to 80 ° C. for 1 to 3 hours, a high inhibitory effect was observed as compared with other extraction temperatures. It was confirmed that it is appropriate to manage within this range.

[Test Example 3: Examination of daily dose of water extract of Ecklonia kurome by comparison of lipase activity inhibitory effect with orlistat]
A lipase activity inhibition test was carried out by the same method as in Test Example 2 using orlistat and a water extract of Ecklonia kurome. Orlistat is a component known to have a lipid absorption inhibitory effect by inhibiting lipase.

As the water extract of Ecklonia kurome, the water extract of Ecklonia kurome prepared at 70 ° C. for 3 hours was used. Table 3 shows the comparison results of both components of the lipase activity inhibition test (IC50).

As shown in Table 3, when the IC50 values were compared, the orlistat was 268.3 times that of the water extract of Ecklonia kurome.

For the effective amount with orlistat, see "J. Zhi et al., Retrospective population-based analysis of the dose-response (fecal fat excretion) relationship of orlistat in normal and obese volunteers Clin Pharmacol Ther. 1994 Jul; 56 (1): 82 In Table II of "-5.", it is shown that the 95% confidence interval of ED50 is 30.2 to 166.0 (mg / day).

Based on the effective amount of orlistat described in the above paper and the results in Table 3, the effective amount (ED50) of the water extract of Ecklonia kurome is converted to 8 g / day to 45 g / day as shown in Table 4. It was confirmed that. Since this numerical range is expected to have the same effect as orlistat, which is a pharmaceutical product, when used as a food, an appropriate health effect is expected if the daily intake is 500 mg or more.

[Test Example 4 Examination of lipase activity depending on the collection time]
The algae of Ecklonia kurome collected at the end of April 2020 in the coastal area of Tottori Prefecture were lightly washed with water and dried in a dryer (manufacturer: ETTAS (As One) model number: OFW-450B) for one day at 37 ° C. .. After drying, 0.5 g was weighed and finely ground with a food processor. 35 mL of distilled water was added to the pulverized chrome, and the mixture was allowed to stand at 70 ° C. for 3 hours in a constant temperature bath for extraction. After extraction, suction filtration was performed with a water flow aspirator using a glass filter to remove seaweed residue from the extract. Then, the extract was suction-filtered using a 3 μm and 0.45 μm filter. The filtered extract was frozen in liquid nitrogen and then lyophilized for 3 days. Then, the activity value (IC50) of each test sample was calculated by the same method as in Test Example 2.

As shown in Table 5, when the chrome collected at the end of April was used as a raw material, a remarkable lipase activity inhibitory effect was shown. Although the results are not shown, the lipase activity inhibitory effect was confirmed even when the chrome collected by March was used as the raw material, but when the chrome collected at the end of April was used as the raw material, it was more effective. It was found to be highly effective.

[Test Example 5 Examination of lipase activity according to growth period]
Of the Ecklonia kurome collected in the coastal area of Tottori Prefecture, the algae, which have a growing period of about 1 to 2 years, were lightly washed with water, drained, and finely crushed with a food processor. 35 mL of distilled water was added to 5 g of the pulverized chrome, and the mixture was allowed to stand at 70 ° C. for 3 hours in a constant temperature bath for extraction. After extraction, suction filtration was performed with a water flow aspirator using a glass filter to remove seaweed residue from the extract. Then, the extract was suction-filtered using a 3 μm and 0.45 μm filter. The extracted extract after filtration was frozen in liquid nitrogen and then freeze-dried for 3 days. Then, the activity value (IC50) of each test sample was calculated by the same method as in Test Example 2.

As shown in Table 6, when chrome having a growth period of about 1 to 2 years was used as a raw material, a higher lipase activity inhibitory effect was shown.

[Test Example 6 Examination of lipase activity by drying after collection]
The kurome collected in the coastal area of Tottori prefecture was lightly washed with water, then dried outdoors in the sun and dried for one day. After drying, 0.5 g of dried Ecklonia kurome was weighed and finely ground with a food processor. 35 mL of distilled water was added to the pulverized chrome, and the mixture was allowed to stand at 70 ° C. for 3 hours in a constant temperature bath for extraction. After water extraction, suction filtration was performed with a water flow aspirator using a glass filter to remove seaweed residue from the extract. Then, the extract was suction-filtered using a 3 μm and 0.45 μm filter. The extracted extract after filtration was frozen in liquid nitrogen and then freeze-dried for 3 days. Then, the activity value (IC50) of each test sample was calculated by the same method as in Test Example 2.

As shown in Table 7, when the kurome was dried before the extraction step and the dried kurome was used as a raw material, a higher lipase activity inhibitory effect was shown.

[Test Example 7 Comparison of lipase activity with ethanol extract]
To prepare the water extract, lightly wash the algae of Kurome collected in the coastal area of Tottori prefecture with water, and use a dryer (manufacturer: ETTAS (As One) model number: OFW-450B) at 37 ° C. It was dried for one day. After drying, 0.5 g of dried Ecklonia kurome was weighed and pulverized with a food processor. 35 mL of distilled water was added to the pulverized chrome, and the mixture was allowed to stand at 70 ° C. for 3 hours in a constant temperature bath for extraction. After extraction, suction filtration was performed with a water flow aspirator using a glass filter to remove seaweed residue from the extract. Then, the extract was filtered in the order of 3 μm and 0.45 μm filters. A lyophilized powder was prepared by freezing the filtered extract with liquid nitrogen and then lyophilizing for 3 days. Then, the activity value (IC50) of the test sample was calculated by the same method as in Test Example 2.

To prepare the ethanol extract, the algae of Ecklonia kurome collected in the coastal area of Tottori Prefecture were lightly washed with water to remove water, and about 5 g was weighed. Then, it was frozen at −30 ° C. and dried for 3 days using a freeze-dryer (manufacturer: EYELA model number: FDU-2110, the same applies to other freeze-drying treatments). After drying, 0.5 g of dried Ecklonia kurome was weighed and pulverized with a food processor. 35 mL of ethanol was added to the crushed chrome, and the mixture was allowed to stand at 70 ° C. for 3 hours in a constant temperature bath for extraction. Since the number of test samples obtained by freeze-drying after ethanol extraction was less than the conditions for water extraction, (0.5 g / 35 mL) x 4 sets were prepared. After extraction, suction filtration was performed with a water flow aspirator using a glass filter to remove seaweed residue from the extract. At this time, the total volume of the extract was 120 mL in total for all four sets. Then, the extract was concentrated and the organic solvent was removed using a rotary evaporator. When it was concentrated 6 times (120 mL → 20 mL), a total of 50-60 mL of distilled water was added in several portions, and concentration and removal of the organic solvent were carried out until no ethanol odor was observed. Finally, the extract was frozen in liquid nitrogen and then lyophilized for 3 days to obtain 37.8 mg lyophilized powder. Then, the activity value (IC50) of the test sample was calculated by the same method as in Test Example 2.
Since the extraction yield with ethanol is considerably lower than that with water, the IC50 was calculated based on the weight of dried kurome before extraction with water or ethanol.

As shown in Table 8, when chrome was used as a raw material, it was confirmed that the water extract was preferable from the viewpoint of the extraction yield and the lipase activity inhibitory effect.

Claims (12)

A composition for inhibiting lipase activity, which contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome). A composition for suppressing lipid absorption, improving stool slippage, or intestinal regulation, which contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome). The composition according to claim 1 or 2, wherein the Ecklonia cava genus Ecklonia cava is a dried product of Ecklonia cava. The composition according to any one of claims 1 to 3, wherein the extraction site of Ecklonia cava is a frond and / or a stem. The composition according to any one of claims 1 to 4, wherein the growing period of Ecklonia cava is 1 to 6 years. The composition according to any one of claims 1 to 5, wherein the extraction temperature for obtaining the extract is 50 to 80 ° C. The composition according to any one of claims 1 to 6, wherein the extraction time for obtaining the extract is 1 to 5 hours. The composition according to any one of claims 1 to 7, which is a pharmaceutical product, a quasi drug, a cosmetic product, or a food or drink. The composition according to any one of claims 1 to 8, wherein the daily intake is 500 mg or more in terms of dry solid content. The following steps:
(1) A method for producing an aqueous extract or processed product of Ecklonia cava, which comprises a step of extracting Ecklonia cava with hot water at 50 to 80 ° C. for 1 to 5 hours. The method according to claim 10, which is a water extract or processed product of Ecklonia cava, which has an enhanced lipid absorption inhibitory effect. Contains an effective amount of a water extract of Ecklonia kurome (scientific name: Ecklonia kurome),
The heating temperature in the extract is 50-80 ° C.
The extraction time in the extract is 1-5 hours.
Processed product of Ecklonia cava.

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