JP2021138625A - External preparation for skin containing component derived from sesame oil - Google Patents

Abstract

To provide an external preparation for skin comprising a component derived from sesame seeds which is excellent in biological safety and is suitably used as a beauty material.SOLUTION: There is provided an external preparation for skin containing a defatted sesame seed or a hydrolysate of the extract by a proteolytic enzyme and a lipolytic enzyme as an active ingredient. There is provided the external preparation for skin in which the proteolytic enzyme is one or more of protease A, protease M, papain, protease P and protease HF.EFFECT: The external preparation for skin has excellent usability and biological safety and has a moisturizing effect or the like to improve skin roughness and aging due to ultraviolet rays, aging or the like.SELECTED DRAWING: Figure 1

Description

The present invention relates to an external preparation for skin containing a component derived from sesame seed, which has excellent biosafety and is suitably used as a cosmetic material.

Conventionally, it is known that sesame seeds are nutritionally excellent, and techniques for using ingredients derived from sesame seeds as materials for foods and drinks and cosmetics have been proposed (for example, Patent Documents 1 to 1). 4).

Japanese Patent Application Laid-Open No. 09-187226 Japanese Patent Application Laid-Open No. 10-045615 Japanese Patent Application Laid-Open No. 10-279428 JP 2011-231505

Although components derived from sesame seeds have been proposed at present, components derived from sesame seeds with further improved usability, effectiveness and stability are required as cosmetological materials.

The present invention is a skin external preparation containing a defatted sesame seed or an extract thereof as a proteolytic enzyme and a hydrolyzate by a lipolytic enzyme as an active ingredient.

According to the present invention, it is possible to provide an external preparation for skin that has excellent usability and biosafety, and further exhibits a moisturizing effect that improves rough skin and aging due to ultraviolet rays, aging, and the like.

It is a figure which shows the evaluation test result of the water content of the stratum corneum. It is a figure which shows the evaluation test result of the water content of the stratum corneum. It is a figure which shows the evaluation test result of the water content of the stratum corneum.

Hereinafter, preferred embodiments of the present invention will be described in detail.
Any sesame seed may be used as the sesame seed used to obtain the hydrolyzate which is the active ingredient of the present invention, but defatted sesame seed is preferable. Further, as the degreased sesame seed, any of white sesame, black sesame, gold sesame, and brown sesame may be used, but white sesame is particularly preferable. As the degreased sesame seeds, any of tropical sesame, subtropical sesame, temperate sesame and the like can be preferably used.

Further, as the sesame seed, those which have been degreased in advance may be used. For degreasing, conventionally known methods such as degreasing using an organic solvent (for example, n-hexane, ethanol, or ethyl acetate) and oil extraction by pressing can be used.

Hereinafter, a method for preparing a hydrolyzate of defatted sesame seeds will be described in detail. First, an extract solution of defatted sesame seeds is obtained. As the extraction solvent, water; lower alcohols such as methanol, ethanol and propanol; polyhydric alcohols such as ethylene glycol, propylene glycol, 1,3-butylene glycol and glycerin; ethyl acetate, butyl acetate, methyl propionate and the like. Esters; ketones such as acetone and methyl ethyl ketone; ethers such as ethyl ether and isopropyl ether; hydrocarbon solvents such as n-hexane, toluene and chloroform, etc., which may be used alone or in admixture of two or more. Used.

In the present invention, a hydrophilic solvent such as water, lower alcohols or polyhydric alcohols is suitable because it is hydrolyzed. Preferred examples of using this hydrophilic solvent include, for example, water, lower alcohols (particularly ethanol), or polyhydric alcohol (particularly 1,3-butylene glycol) used alone, or water and lower alcohols. Examples include the use of a mixed solvent with (particularly ethanol) or a mixed solvent with water and polyhydric alcohols (particularly 1,3-butylene glycol). Among them, water alone or water and 1,3-butylene. A mixed solvent of glycol is particularly preferable.

In the present invention, when preparing a sesame seed extract solution, it is preferable to adjust the pH using an alkali adjusting agent. Examples of the alkali preparation include sodium salts such as sodium hydroxide and sodium carbonate, and potassium salts such as potassium hydroxide.

Hydrolysis treatment is performed in parallel with the above-mentioned extraction treatment or after the extraction treatment. In the present invention, a hydrolysis treatment is carried out by combining a proteolytic enzyme and a lipolytic enzyme for the purpose of increasing the amino acid content during hydrolysis and improving the stability.

The proteolytic enzyme is not particularly limited, but among them, any one or more of protease A, protease M, papaine, protease P and protease HF having both endoprotease activity and exoprotease activity, endoprotease. It is preferable to use any one or more of active samoase and protin, or peptidase having exoprotease activity. Examples of the lipolytic enzyme include microbial-derived lipase and animal-derived lipase.

The amount of the enzyme added is preferably in the range of 0.000000001 to 10% by weight in total, more preferably in the range of 0.000001 to 1.0% by weight, based on the solid content of the site of use of each plant. be.

Examples of skin external preparations (cosmetics, non-medicinal products, external medicines, etc.) containing the hydrolyzate according to the present invention include emulsions, creams, lotions, essences, packs, lipsticks, foundations, liquid foundations, and make-up press powders. , Body shampoo, shampoo, conditioner, conditioner, conditioner, hair cream, lip cosmetics, soap, washing pigment, cleaning agent, etc., and further, bathing agent, etc.

When the hydrolyzate according to the present invention is used as a compounding agent for external preparations for skin (cosmetics, non-pharmaceutical products, external medicines, etc.), in addition to the essential hydrolyzate, for example, an oily component, a surfactant, etc. (Synthetic, natural products), moisturizers, thickeners, emulsifiers or emulsifying aids, preservatives / bactericides, powder components, UV absorbers, antioxidants, pigments, fragrances, chelating agents, and other natural products Derived physiologically active ingredients and the like can be appropriately blended as needed. Further, as long as the effectiveness and features of the hydrolyzate according to the present invention are not impaired, it may be blended in combination with other physiologically active ingredients.

Oily ingredients include, for example, olive oil, jojoba oil, castor oil, sesame oil, soybean oil, rice oil, rice germ oil, palm oil, palm oil, cacao oil, meadowfoam oil, sheer butter, tea tree oil, avocado oil, macadamia nuts. Oils, plant-derived fats and oils such as plant-derived squalane; animal-derived fats and oils such as mink oil and turtle oil; waxes such as beeswax, carnauba wax, rice wax, and lanolin; liquid paraffin, vaseline, paraffin wax, squalane, etc. Hydrocarbons; fatty acids such as myristic acid, palmitic acid, stearic acid, oleic acid, isostearic acid, cis-11-eicosenoic acid; higher alcohols such as lauryl alcohol, cetanol, stearyl alcohol; isopropyl myristate, isopropyl palmitate , Butyl oleate, 2-ethylhexyl glyceride, synthetic esters such as higher fatty acid octyldodecyl (octyldodecyl stearate, etc.) and synthetic triglycerides.

Examples of the surfactant include polyoxyethylene alkyl ether, polyoxyethylene fatty acid ester, polyoxyethylene sorbitan fatty acid ester, glycerin fatty acid ester, polyglycerin fatty acid ester, polyoxyethylene glycerin fatty acid ester, polyoxyethylene hydrogenated castor oil, and poly. Nonionic surfactants such as oxyethylene sorbitol fatty acid esters; fatty acid salts, alkyl sulfates, alkylbenzene sulfonates, polyoxyethylene alkyl ether sulfates, polyoxyethylene fatty amine sulfates, polyoxyethylene alkylphenyl ether sulfates, Anionic surfactants such as polyoxyethylene alkyl ether phosphates, α-sulfonated fatty acid alkyl ester salts, polyoxyethylene alkyl phenyl ether phosphates; quaternary ammonium salts, primary to tertiary fatty amine salts, tri Cationic surfactants such as alkylbenzylammonium salt, alkylpyridinium salt, 2-alkyl-1-alkyl-1-hydroxyethylimidazolinium salt, N, N-dialkylmorphonium salt, polyethylene polyamine fatty acid amide salt; N, N-dimethyl-N-alkyl-N-carboxymethylammoniobetaine, N, N, N-trialkyl-N-alkylene ammoniocarboxybetaine, N-acylamide propyl-N', N'-dimethyl-N'- An amphoteric surfactant such as β-hydroxypropylammoniosulfobetaine can be used.

Examples of emulsifiers or emulsifying aids include stevia derivatives such as enzyme-treated stevia, saponins or derivatives thereof, casein or salts thereof (sodium, etc.), sugar-protein complexes, sucrose or its fatty acid esters, lactose, and water-soluble soybeans. Sexual polysaccharides, soybean-derived protein-polysaccharide complexes, lanolin or derivatives thereof, cholesterol, stevia derivatives (stevia enzyme-treated products, etc.), silicates (aluminum, magnesium, etc.), carbonates (calcium, sodium, etc.) saponins and theirs. Derivatives, lecithin and its derivatives (hydrogenized lecithin, etc.), lactic acid bacteria fermented rice and the like can also be blended.

Examples of the moisturizing agent include glycerin, propylene glycol, dipropylene glycol, 1,3-butylene glycol, polyethylene glycol, sorbitol, xylitol, sodium pyrrolidone carboxylate and the like, and saccharides such as trehalose and mucopolysaccharides (for example, hyalurone). Acids and their derivatives, chondroitin and its derivatives, heparin and its derivatives, etc.), elastin and its derivatives, collagen and its derivatives, NMF-related substances, lactic acid, urea, higher fatty acids octyldodecyl, seaweed extracts, various amino acids and their derivatives Can be mentioned.

Examples of the thickener include components derived from brown algae such as alginic acid, agar, carrageenan and fucoidan, green algae or red algae; polysaccharides such as pectin, locust bean gum, aloe polysaccharides and alkaligenes-producing polysaccharides; xanthan gum, tragant gum, guar gum and the like. Gum; Cellulose derivatives such as carboxymethyl cellulose and hydroxyethyl cellulose; Synthetic polymers such as polyvinyl alcohol, polyvinyl pyrrolidone, carboxyvinyl polymer, alginic acid / methacrylic acid copolymer; Hyaluronic acid and its derivatives; Polyglutamic acid and its derivatives And so on.

Examples of preservatives and bactericides include urea; paraoxybenzoic acid esters such as methyl paraoxybenzoate, ethyl paraoxybenzoate, propyl paraoxybenzoate, and butyl paraoxybenzoate; phenoxyethanol, dichlorophenol, hexachlorophene, chlorhexidine hydrochloride, and benza chloride. There are luconium, salicylic acid, ethanol, undesyleneic acid, phenols, jamar (imidazodeini ruurea), 1,2-pentanediol, various essential oils, bark dry distillate, radish fermented liquid and the like.

Examples of powder components include cericite, titanium oxide, talc, kaolin, bentonite, zinc oxide, magnesium carbonate, magnesium oxide, zirconium oxide, barium sulfate, silicic anhydride, mica, nylon powder, polyethylene powder, silk powder, and cellulose. There are system powders, grain powders (rice, wheat, corn, talc, etc.), beans (soybeans, talc, etc.) powders, etc.

Examples of the ultraviolet absorber include ethyl paraaminobenzoate, ethylhexyl paradimethylaminobenzoate, amyl salicylate and its derivatives, 2-ethylhexyl paramethoxycinnamate, octyl silicate, oxybenzon, 2,4-dihydroxybenzophenone, 2-hydroxy-4. -Methoxybenzophenone-5-sulfonate, 4-tershally butyl-4-methoxybenzoylmethane, 2- (2-hydroxy-5-methylphenyl) benzotriazole, urocanic acid, ethyl urocanate, aloe extract, etc. ..

Antioxidants include, for example, butylhydroxyanisole, butylhydroxytoluene, propyl gallate, vitamin E and derivatives thereof (for example, vitamin E nicotinate, vitamin E linoleate, etc.).

Examples of the chelating agent include ethylenediamine hydroxyethyl triacetate trisodium, edetonic acid or salts thereof, gluconic acid, phytic acid, sodium polyphosphate, sodium metaphosphate, tetrasodium hydroxyetandiphosphonate and the like.

Whitening agents include t-cycloamino acid derivatives, kodiic acid and its derivatives, ascorbic acid and its derivatives, hydroquinone or its derivatives, ellagic acid and its derivatives, nicotinic acid and its derivatives, resorcinol derivatives, tranexamic acid and its derivatives, 4 -Potasium methoxysalicylic acid, magnolignan (5,5'-dipropyl-biphenyl-2,2'-diol), hydroxybenzoic acid and its derivatives, vitamin E and its derivatives, α-hydroxy acid, AMP (adenosine monophosphate) , Adenosine monophosphate), and these may be blended alone or in combination of two or more.

Furthermore, as natural product-derived components, plant-extracted components, seaweed-extracted components, microbial-derived components, and / or animal-derived components that can be blended in skin external preparations such as cosmetics and quasi-drugs are blended. Is also good.

Next, the present invention will be described in more detail with reference to Production Examples, Test Examples and Formulation Examples, but the present invention is not limited thereto. In the following, all parts mean parts by weight, and% means all parts by weight.

Production example 1. Sesame seed extract hydrolyzed solution Sesamum indicum, a plant belonging to the genus Sesamum in the family Pedaliaceae, is crushed, and 1000 g of purified water is added to 100 g of the crushed product at 80 ° C. After extraction, the insoluble material was removed by filtration, and 3,000 U / min of lipase, which is a lipolytic enzyme, was added to the filtrate. The enzyme treatment was carried out by keeping the lipase at the optimum pH at 40 ° C. for 2 hours. Subsequently, 6,000 U / min of protease A (manufactured by Amano Enzyme Co., Ltd.), which is a proteolytic enzyme, was added to the enzyme treatment solution obtained here. The enzyme treatment was carried out by maintaining the protease A at an optimum pH of 6 to 9 at 40 ° C. for 18 hours. The insoluble matter of the obtained enzyme treatment solution was filtered to obtain 1350 g (solid content concentration 0.62%) of a pale yellowish brown transparent sesame seed extract hydrolyzed solution.

Production example 2. Hydrolyzed solution of sesame seed extract In the production process of Production Example 1, protease M (manufactured by Amano Enzyme Co., Ltd.) is used instead of protease A as a proteolytic enzyme, and treatment is performed at the optimum pH 5 to 7. By the same step as in Example 1, 1290 g (solid content concentration 0.65%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 3. Hydrolyzed solution of sesame seed extract In the production process of Production Example 1, papain (manufactured by Amano Enzyme Co., Ltd.) is used as the proteolytic enzyme instead of protease A, and the solution is treated at the optimum pH of 6 to 8. By the same step as in No. 1, 1310 g (solid content concentration 0.60%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 4. Hydrolyzed solution of sesame seed extract In the production process of Production Example 1, protease P (manufactured by Amano Enzyme Co., Ltd.) is used instead of protease A as a proteolytic enzyme, and treatment is performed at the optimum pH 6 to 9. By the same step as in Example 1, 1270 g (solid content concentration 0.68%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 5. Hydrolyzed solution of sesame seed extract In the production process of Production Example 1, protease HF (manufactured by Amano Enzyme Co., Ltd.) is used instead of protease A as a proteolytic enzyme, and treatment is performed at the optimum pH 3 to 4. By the same step as in Example 1, 1320 g (solid content concentration 0.65%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 6. Sesame seed extract hydrolyzed solution In the production process of Production Example 1, Samoase (manufactured by Amano Enzyme Co., Ltd.) is used instead of protease A as a proteolytic enzyme, and the solution is treated at an optimum pH of 7 to 8. By the same step as in No. 1, 1330 g (solid content concentration 0.66%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 7. Sesame seed extract hydrolyzed solution In the production process of Production Example 1, Protin (manufactured by Amano Enzyme Co., Ltd.) is used instead of Protease A as a proteolytic enzyme, and the solution is treated at the optimum pH 7 to 8. By the same step as in No. 1, 1210 g (solid content concentration 0.60%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Production example 8. Hydrolyzed solution of sesame seed extract In the production process of Production Example 1, peptidase (manufactured by Amano Enzyme Co., Ltd.) is used instead of protease A as a proteolytic enzyme, and the solution is treated at an optimum pH of 6 to 8. By the same step as in No. 1, 1290 g (solid content concentration: 0.63%) of a pale yellowish brown transparent sesame seed extract hydrolysis solution was obtained.

Comparative manufacturing example 1. Sesame seed extract hydrolyzed solution Sesamum indicum seeds (sesame seeds degreased by squeezed oil) of the sesame family Sesamum indicum are crushed, 1000 g of purified water is added to 100 g of the crushed product, and extracted at 80 ° C. , Insoluble matter was removed by filtration, and protease A (manufactured by Amano Enzyme Co., Ltd.) was added to the filtrate. The enzyme treatment was carried out by holding the protease A at an optimum pH of 5 to 7 at 50 ° C. for 18 hours. The insoluble matter of the obtained enzyme treatment solution was filtered to obtain a pale yellowish brown transparent sesame seed extract hydrolyzed solution (solid content concentration: 0.69%).

Comparative manufacturing example 2. Sesame seed extract hydrolyzed solution Sesamum indicum seeds (sesame seeds degreased by squeezed oil) of the sesame family Sesamum indicum are crushed, 1000 g of purified water is added to 100 g of the crushed product, and extracted at 80 ° C. , Insoluble matter was removed by filtration, and protease M (manufactured by Amano Enzyme Co., Ltd.) was added to the filtrate. The enzyme treatment was carried out by holding the protease M at an optimum pH of 6 to 9 at 45 ° C. for 18 hours. The insoluble matter of the obtained enzyme treatment solution was filtered to obtain a pale yellowish brown transparent sesame seed extract hydrolyzed solution (solid content concentration: 0.64%).

Comparative manufacturing example 3. Sesame seed extract hydrolyzed solution Sesamum indicum seeds (sesame seeds degreased by squeezed oil) of the sesame family Sesamum indicum are crushed, 1000 g of purified water is added to 100 g of the crushed product, and extracted at 80 ° C. , Insoluble matter was removed by filtration, and protease M (manufactured by Amano Enzyme Co., Ltd.) and sesame were added to the filtrate. Enzymatic treatment was performed by holding at 45 ° C. for 18 hours at the optimum pH of Protease M and papain. The insoluble matter of the obtained enzyme treatment solution was filtered to obtain a pale yellowish brown transparent sesame seed extract hydrolyzed solution (solid content concentration: 0.68%).

Test example 1. Evaluation test of water content in the stratum corneum [Preparation of sample solution]
[Table 1]

[Test plot setting]
(1) No application area (2) Control lotion application area
(3) Coating section of Production Example 1
[Test method]
As described above, three test groups (1) to (3) were set on the medial part of the forearm of the subjects (three women in their twenties), and after measuring the water content of the stratum corneum in each test group, the sample was applied. It started. An appropriate amount of the sample was applied to each test group twice a day.
The water content of the stratum corneum was measured weekly from the test start date. The test period was 2 weeks from the start date of the test.
[result]
The amount of change in the amount of water in the stratum corneum of each test site of each subject was calculated by subtracting the variation (value in the non-applied group) depending on each measurement day.

The test results are shown in FIGS. 1 to 3. As shown in those figures, it was confirmed that the water content of the stratum corneum was improved in all the subjects to which the hydrolyzate according to the present invention was applied.

Test example 2. Usability evaluation test 5.0% of the hydrolyzate solution of any of Production Examples 2 to 8 in place of the hydrolyzate solution of Production Example 1 in the sample 1 of the present invention shown in Table 1 above and the sample 1 of the present invention. Samples 2 to 8 of the present invention containing the above preparations were prepared. Further, in Sample 1 of the present invention, Comparative Samples 1 to 3 were prepared by blending 5.0% of any of the hydrolyzate solutions of Comparative Production Examples 1 to 3 in place of the hydrolyzate solution of Production Example 1.
[Test method]
Five randomly selected men and women aged 25 to 60 years were used as subjects, and samples 1 to 8 of the present invention and comparative samples 1 to 3 were set as test groups on the upper arms of each subject, and samples 1 to 1 of the present invention were set. 8. The feeling of use when Comparative Samples 1 to 3 were applied to each test group twice a day (morning and evening) for 1 week was evaluated. The feeling of use is evaluated on a five-point scale of A: very good, B: good, C: normal, D: slightly bad, and E: bad, in terms of feel (a) in the hand and elongation (b) during application. bottom. The sticky feeling (c) was evaluated on a five-point scale: A: not felt, B: almost not felt, C: slightly felt, D: felt, and E: very felt.

The results of Test Example 2 are shown in Table 2. The numbers in Table 2 indicate the number of respondents.

As shown in Table 2, it was confirmed that the samples 1 to 8 of the present invention had an excellent usability as compared with the comparative samples 1 to 3.

Test example 3. ^{Evaluation test of epidermal cell activation effect Human epidermal cell NHEK was seeded in 5 × 10 3} cells / hole in a 96-well microplate containing HuMedia KG2 medium (manufactured by Kurabo) under the conditions of 37 ° C. and 5.0% CO _2. After pre-culturing under the conditions for 1 day, HuMedia KG2 medium containing the hydrolyzate solution according to Production Examples 1 to 8 and Comparative Production Example 1 according to the present invention was added as a sample solution, and the cells were cultured under the same conditions for another 2 days. Here, the concentration of each sample solution was adjusted so that the concentration of the solution with respect to the medium was 2%. The medium was then removed, 0.03% MTT was added and kept at 37 ° C. for 1 hour, the formazan produced was extracted with isopropanol and wavelengthd using a microplate reader (Model 680, Bio-Rad). The MTT value was measured at 570-630 nm. In the case of no sample addition (control) in which PBS (-) was added instead of the sample solution, the same operation as above was performed, and the relative value of the MTT value at the time of addition of each sample to the MTT value obtained here was obtained. , Epidermal cell MTT activity rate (%). A similar test was also performed when 100 mM glucose was added as a positive control instead of the sample solution in order to confirm whether the test system was functioning normally.

The results of Test Example 3 are shown in Table 3.
[Table 3]

As shown in Table 3, it was confirmed that Samples 1 to 8 of the present invention had an excellent epidermal cell activating effect as compared with Comparative Sample 1.

Test example 4. Evaluation test of water content in the stratum corneum A test plot was set on the medial side of the forearm of the subject, and the water content in the stratum corneum in each test plot was measured (initial value). Next, using the hydrolyzate solution of Production Examples 1 to 8 and the hydrolyzate solution of Comparative Production Examples 1 to 3 as test solutions, 100 μL of the hydrolyzate solution was applied to each test group and then lightly wiped off, and then the stratum corneum moisture was continuously obtained. The amount was measured. The initial value of each test group was subtracted, and the water content of the stratum corneum was quantified.

The results of Test Example 4 are shown in Table 4.
[Table 4]

As shown in Table 4, it was confirmed that the hydrolyzate solutions of Production Examples 1 to 8 according to the present invention had higher moisturizing sustainability than the hydrolyzate solutions of Comparative Examples 1 to 3.

Prescription example 1. Toner [Ingredients] Part Squalene 1.0
Methylparaben 0.1
Hydrolyzed solution of Production Example 1 5.0
Ethanol 5.0
Glycerin 5.0
1,3-butylene glycol 5.0
Citric acid 0.2
Amount that makes the total amount of purified water 100 copies Appropriate amount of fragrance

Prescription example 2. Toner A lotion was obtained in the same manner as in Formulation 1, except that the hydrolyzate solution of Production Example 2 was contained in the lotion of Formulation Example 1 instead of the hydrolyzate solution of Production Example 2.

Prescription example 3. Toner A lotion was obtained in the same manner as in Formulation 1, except that the hydrolyzate solution of Production Example 3 was contained in the lotion of Formulation Example 1 instead of the hydrolyzate solution of Production Example 3.

Prescription example 4. Toner A lotion was obtained in the same manner as in Formulation 1 except that the hydrolyzate solution of Production Example 4 was contained in place of the hydrolyzate solution of Production Example 1 contained in the lotion of Formulation Example 1.

Prescription example 5. Toner A lotion was obtained in the same manner as in Formulation 1 except that the hydrolyzate solution of Production Example 5 was contained in place of the hydrolyzate solution of Production Example 1 contained in the lotion of Formulation Example 1.

Prescription example 6. Toner A lotion was obtained in the same manner as in Formulation 1 except that the hydrolyzate solution of Production Example 6 was contained in place of the hydrolyzate solution of Production Example 1 contained in the lotion of Formulation Example 1.

Prescription example 7. Toner A lotion was obtained in the same manner as in Formulation 1 except that the hydrolyzate solution of Production Example 7 was contained in place of the hydrolyzate solution of Production Example 1 contained in the lotion of Formulation Example 1.

Prescription example 8. Toner A lotion was obtained in the same manner as in Formulation 1, except that the hydrolyzate solution of Production Example 8 was contained in place of the hydrolyzate solution of Production Example 1 contained in the lotion of Formulation Example 1.

Prescription example 9. Toner [Ingredients] Part 10.0 Hydrolyzed solution of Production Example 1
Ethanol 10.0
Glycerin 3.0
1,3-butylene glycol 2.0
Methylparaben 0.2
Dipotassium glycyrrhizinate 0.1
Citric acid 0.1
Sodium citrate 0.3
Carboxyvinyl polymer 0.1
Xanthan gum 0.1
Appropriate amount of fragrance Potassium hydroxide Appropriate amount Purified water 100 parts in total

Prescription example 10. Cream [Ingredients] Part Liquid paraffin 6.0
Hexalan 4.0
Sesame oil 1.0
Polyoxyethylene (20) sorbitan monolauryl acid ester 1.0
Oil-based glyceryl stearate 1.0
Soy lecithin 1.5
Hydrolyzed solution of Production Example 1 3.0
Lactic acid bacteria fermented rice 2.0
L-ascorbic acid-2-glucoside 2.0
Potassium hydroxide 0.5
Dipotassium glycyrrhizinate 0.1
Glycerin 3.0
1,3-butylene glycol 2.0
Carboxymethyl cellulose 0.3
Sodium hyaluronate 0.01
Amount that makes the total amount of purified water 100 copies Appropriate amount of fragrance

Prescription example 11. Cream A milky lotion is obtained in the same manner as in Formulation Example 10 except that 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 parts of potassium hydroxide are replaced with 3.0 parts of arbutin in the components of Formulation Example 10. rice field.

Prescription example 12. Cream In the ingredients of Formulation Example 10, cream is prepared in the same manner as in Formulation Example 10 except that 2.0 parts of tranexamic acid is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide. Obtained.

Prescription example 13. Cream Cream in the same manner as in Formulation Example 10 except that 3.0 parts of nicotinic acid amide is used instead of 2.0 parts of L-ascorbic acid-2-glucoside and 0.5 part of potassium hydroxide among the components of Formulation Example 10. Got

Prescription example 14. Beauty Essence [Ingredients] Part Hydrolyzed Solution of Production Example 1 3.0
Ethanol 6.0
PEG-60 hydrogenated castor oil 1.0
Glycerin 3.0
Dimethicone Copolyol Methyl 0.4
Glycosyl trehalose 5.0
Hydrolyzed hydrogenated starch 5.0
Xanthan gum 2.0
Raffinose 3.0
Triethanolamine 1.0
Methylparaben 0.2
Appropriate amount of fragrance Amount of purified water totaling 100 parts

Prescription example 15. Foundation [Ingredients] Stearic acid 2.4
Propylene glycol monostearate 2.0
Setostearyl alcohol 0.2
Liquid lanolin 2.0
Liquid paraffin 3.0
Isopropyl myristate 8.5
Propylparaben 0.05
Hydrolyzed solution of Production Example 1 5.0
Sodium Carboxymethyl Cellulose 0.2
Bentonite 0.5
Propylene glycol 4.0
Triethanolamine 1.1
Methylparaben 0.1
Titanium oxide 8.0
Talc 4.0
Appropriate amount of colored pigment Amount of purified water totaling 100 parts

Prescription example 16. Pack L-ascorbic acid-2-glucoside 2.0
Glycerin 5.0
1,3 Butylene glycol 10.0
Potassium hydroxide 0.5
Hydrolyzed solution of Production Example 1 3.0
Sodium citrate 0.1
Sodium hyaluronate 0.1
Amount of purified water totaling 100 parts

Prescription example 17. Facial cleanser Potassium laurate 2.0
Potassium myristate 16.0
Cocoyl glycine potassium 8.0
Squalene 0.5
Coconut oil 2.0
Glycerol monostearate 2.0
Hydrolyzed solution of Production Example 1 3.0
Disodium edetate 0.5
Phenoxyethanol 0.5
Dipropylene glycol 0.5
Glycerin 10.0
Amount of purified water totaling 100 parts

Prescription example 18. Shampoo [Ingredients] Part N-Palm Oil Fatty Acid Methyl Taurine Sodium 10.0
Polyoxyethylene (3) Alkyl Ether Sodium Sulfate 20.0
Betaine Lauryl Dimethylamino Acetate 10.0
Coconut oil fatty acid diethanolamide 4.0
Methylparaben 0.1
Citric acid 0.1
Hydrolyzed solution of Production Example 1 2.0
1,3-butylene glycol 2.0
Amount of purified water totaling 100 parts

Example 19. Hair Conditioner [Ingredients] Part Polyoxyethylene (10) Hardened Castor Oil 1.0
Distearyl dimethylammonium chloride 1.5
Stearyltrimethylammonium chloride 2.0
Glyceryl 2-ethylhexanoate 1.0
Cetanol 3.2
Stearyl alcohol 1.0
Methylparaben 0.1
Hydrolyzed solution of Production Example 1 2.0
1,3-butylene glycol 5.0
Amount of purified water totaling 100 parts

Prescription example 20. Body shampoo [Ingredients] Part N-lauroylmethylalanine sodium 25.0
Coconut oil fatty acid potassium solution (40%) 26.0
Coconut oil fatty acid diethanolamide 3.0
Methylparaben 0.1
Hydrolyzed product of Production Example 1 5.0
1,3-butylene glycol 2.0

Claims (3)

An external preparation for skin containing a proteolytic enzyme of defatted sesame seeds or an extract thereof and a hydrolyzate of a lipolytic enzyme as an active ingredient. The external preparation for skin according to claim 1, wherein the proteolytic enzyme is any one or more of protease A, protease M, papain, protease P, and protease HF. The external preparation for skin according to claim 1, wherein the proteolytic enzyme is any one or more of protease A and protease M.

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