JP2021136905A5 - - Google Patents
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- Publication number
- JP2021136905A5 JP2021136905A5 JP2020036812A JP2020036812A JP2021136905A5 JP 2021136905 A5 JP2021136905 A5 JP 2021136905A5 JP 2020036812 A JP2020036812 A JP 2020036812A JP 2020036812 A JP2020036812 A JP 2020036812A JP 2021136905 A5 JP2021136905 A5 JP 2021136905A5
- Authority
- JP
- Japan
- Prior art keywords
- amino acid
- seq
- acid sequence
- mutant
- isoprene
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 125000003275 alpha amino acid group Chemical group 0.000 claims 23
- CBIDRCWHNCKSTO-UHFFFAOYSA-N prenyl diphosphate Chemical compound CC(C)=CCO[P@](O)(=O)OP(O)(O)=O CBIDRCWHNCKSTO-UHFFFAOYSA-N 0.000 claims 20
- RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical compound CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 claims 14
- 108010075483 isoprene synthase Proteins 0.000 claims 12
- 108090000765 processed proteins & peptides Proteins 0.000 claims 10
- 229920001184 polypeptide Polymers 0.000 claims 9
- 102000004196 processed proteins & peptides Human genes 0.000 claims 9
- 108090000623 proteins and genes Proteins 0.000 claims 9
- ASUAYTHWZCLXAN-UHFFFAOYSA-N prenol Chemical compound CC(C)=CCO ASUAYTHWZCLXAN-UHFFFAOYSA-N 0.000 claims 8
- 230000000694 effects Effects 0.000 claims 7
- 238000000034 method Methods 0.000 claims 6
- 238000012216 screening Methods 0.000 claims 6
- 239000000758 substrate Substances 0.000 claims 6
- 150000001413 amino acids Chemical class 0.000 claims 5
- 238000006467 substitution reaction Methods 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 4
- 125000003729 nucleotide group Chemical group 0.000 claims 4
- 230000010261 cell growth Effects 0.000 claims 3
- 238000012217 deletion Methods 0.000 claims 3
- 230000037430 deletion Effects 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 230000002194 synthesizing effect Effects 0.000 claims 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000004663 cell proliferation Effects 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 108060004127 isopentenyl phosphate kinase Proteins 0.000 claims 1
- 125000001844 prenyl group Chemical group [H]C([*])([H])C([H])=C(C([H])([H])[H])C([H])([H])[H] 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020036812A JP7565569B2 (ja) | 2020-03-04 | 2020-03-04 | 変異型イソプレン合成酵素及びそのスクリーニング方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020036812A JP7565569B2 (ja) | 2020-03-04 | 2020-03-04 | 変異型イソプレン合成酵素及びそのスクリーニング方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2021136905A JP2021136905A (ja) | 2021-09-16 |
| JP2021136905A5 true JP2021136905A5 (https=) | 2023-06-30 |
| JP7565569B2 JP7565569B2 (ja) | 2024-10-11 |
Family
ID=77666670
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020036812A Active JP7565569B2 (ja) | 2020-03-04 | 2020-03-04 | 変異型イソプレン合成酵素及びそのスクリーニング方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP7565569B2 (https=) |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2816306A1 (en) * | 2010-10-27 | 2012-05-03 | Danisco Us Inc. | Isoprene synthase variants for improved production of isoprene |
| US8895277B2 (en) * | 2012-05-02 | 2014-11-25 | Danisco Us Inc. | Legume isoprene synthase for production of isoprene |
| CN104762275B (zh) * | 2015-04-15 | 2018-11-09 | 中国科学院天津工业生物技术研究所 | 一种具有催化dma合成dmapp功能的酶及其应用 |
-
2020
- 2020-03-04 JP JP2020036812A patent/JP7565569B2/ja active Active
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