JP2021045120A - Germination method of upright nemacystus decipiens from discal nemacystus decipiens - Google Patents

Abstract

To provide a germination method of upright Nemacystus decipiens from discal Nemacystus decipiens.SOLUTION: Provided is a germination method of upright Nemacystus decipiens from discal Nemacystus decipiens, characterized by irradiating discal Nemacystus decipiens with (A) light in the wavelength range of 400 nm or more and less than 500 nm and/or (B) light in the wavelength range of 500 nm or more and less than 600 nm, each of which has a light quantity of 25 μmol/m2/s or more, as well as with (C) light in the wavelength range of 600 nm or more and less than 700 nm, which has a light quantity of 35 μmol/m2/s or less, in the presence or absence of white light.SELECTED DRAWING: Figure 2

Description

The present invention relates to a method for germinating a straight solid from a disc-shaped body of mozuku in the presence or absence of white light.

Traditionally, mozuku has been eaten in various parts of Japan, but in recent years it has been recognized as a health food and natural food, and demand is increasing. At present, cultivated mozuku is mainly distributed, and especially in Japan, many cultivated mozuku are distributed in Okinawa. And in order to further increase the yield of mozuku, efforts are being made to develop aquaculture technology.

The method of cultivating mozuku in Okinawa, especially the step of sprouting the disc of mozuku to grow into mozuku, is, for example, (1) the step of adhering the disc of mozuku to a net or the like to prepare a seed net. (2) The step of installing the seed net in the sea, (3) the step of sprouting a straight solid from the disc-shaped body of mozuku attached to the seed net, (4) the step of growing the straight solid and growing it into mozuku, (5) ) There is a method of sequentially performing the process of harvesting the grown mozuku.

Many techniques related to the ecology and cultivation of Okinawa mozuku have been reported, for example, a report on appropriate illuminance for promoting the growth of mozuku (Non-Patent Document 1), a report on the cultivation technology of Okinawa mozuku (Non-Patent Document 2), and Okinawa. A report summarizing the effects of water temperature, light intensity, salt content and nutrients on the growth of Mozuku's straight three-dimensional body (sprouting algae) (Non-Patent Document 3), and a report on the relationship between the sprouting of Mozuku's straight three-dimensional body and water temperature (Non-patent). There are reports on the low water temperature limit of Okinawa mozuku disc-shaped body growth (Non-Patent Document 5).

However, in the above-mentioned "(3) Step of germinating a straight solid from a mozuku disc attached to a seed net" of the mozuku aquaculture method, the conditions for germinating a straight solid from the mozuku disc are still clear. The current situation is that it has not been clarified. Therefore, in order to ensure a stable harvest of mozuku, there is a need for a method for efficiently germinating straight solids from the disc-shaped body of mozuku.

Takeshi Toma "Okinawa's seaweed and seaweed (natural environment, aquaculture, 250 species of seaweed)" Publishing Building Mugen 237-260 (2012) Iwao Niimura "Basic Research on Okinawa Mozuku Cultivation" Hokkaido University Examination Dissertation (1976) "Growth characteristics of Okinawa mozuku under indoor culture" Okinawa Prefectural Fisheries and Marine Technology Center 2011 Research and dissemination information Satoshi Moromizato et al. "Indoor growth of mozuku algae and culture of filamentous bodies" 2000 Okinawa Prefectural Fisheries Experimental Station Business Report (2000) "Low water temperature limit of Okinawa mozuku disc-shaped body growth" Okinawa Prefectural Fisheries and Marine Technology Center 2012 Research and dissemination information

An object of the present invention is to provide a method for germinating a straight solid from a disc-shaped body of mozuku in the presence or absence of white light.

As a result of intensive research to solve the above problems, the present inventors irradiate the disk-shaped body of Mozuku with a combination of a specific amount of light and light in a wavelength range in the presence or absence of white light. By doing so, it was found that the straight solids germinate efficiently from the disc-shaped body of Mozuku. Based on these findings, the present inventors have further studied and completed the present invention.
That is, the present invention includes the following configurations.
[1] In the presence or absence of white light
The disk-shaped body of Mozuku, light amount below the light, and the light amount is less than 35 [mu] mol ^{/ m} 2 / s in light and / or the following (A) is 25 [mu] mol ^{/ m} 2 / s or more, respectively (B) (C) A method of germinating a straight solid from a disc-shaped body of a mozuku, which is characterized by irradiating with the light of.
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm [2] The method for germinating a straight solid from the disc-shaped body of Mozuku according to the above [1], which comprises irradiating only the light of A) and / or the light of (B).
[3] The light of (A) and the light of (B) having a total amount of light of 50 μmol / m ^{2 / s or more on the plate-shaped body of mozuku,}
The method for germinating a straight solid from the disc-shaped body of mozuku according to the above [1], which comprises irradiating with the light of (C) having a light amount of 35 μmol / m ^{2 / s or less.}
[4] A straight solid from the mozuku board according to any one of the above [1] to [3], wherein the light irradiating the mozuku board is LED-derived light. How to germinate.
[5] A method for germinating a straight solid from a plate-shaped body of mozuku under irradiation with white light, characterized in that the irradiation of light according to the following (C) is reduced by a material.
(C) Light in the wavelength range of 600 nm or more and less than 700 nm [6] In the method of germinating a straight solid from a plate-like body of Mozuku under irradiation with white light, the light of (A) and / or the light of (B) below. A method characterized by enhancing or diminishing the irradiation of light according to (C) below.
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm

In the method of germinating a straight solid from a mozuku disc-like body of the present invention, the light contained in the mozuku disc-shaped body in a specific wavelength range in the presence or absence of white light can be used alone or in a specific amount of light. By irradiating in combination, it has the effect of efficiently germinating straight solids from the disc-shaped body of mozuku.

Shows the life cycle of mozuku. In Example 2, the photograph of the mozuku seed net thread taken on the 14th day of the culture is shown.

In the present invention, "Mozuku" usually refers to algae belonging to the genus Mozuku of the order Mozuku, the genus Mozuku, or the genus Mozuku of the order Mozuku, and specifically belongs to the genus Mozuku. Okinawa mozuku (Cladosiphon okamuranus), Futomozuku (Tinocladia crassa), Ishimozuku (Sphaerotricia davaricata) or Mozuku belonging to the genus Mozuku (Nemacystus decipi)

The "Mozuku disc" (hereinafter, also simply referred to as "disc") in the present invention is a grown zygote formed by joining a female gamete and a male gamete of Mozuku, or , Neutral gametes originating from the neutral compound sac of Mozuku are grown (see also FIG. 1).

In the present invention, "germinating a straight solid from a disc-shaped body" usually means a state in which a large number of assimilated yarns rise from the center of the disc-shaped body, and the assimilated yarns gather and grow, that is, a disc-shaped body. A state in which a straight solid is formed from the body. If the germination conditions are not met, the disc-shaped body of mozuku will not gather assimilated threads and will become a neutral compound ascus as it is. Then, neutral zoospores are generated from the neutral ascus and form a disc-like body again (see also FIG. 1).

One of the present inventions is the light and / or (B) of the following (A) in ^{which the amount of light is 25 μmol / m 2} / s or more, respectively, in the disk-shaped body of Mozuku in the presence or absence of white light. It is a method of germinating a straight solid from a disc-shaped body of Mozuku, which is characterized by irradiating with light and the light of the following (C) having a light amount of 35 μmol / m ^{2 / s or less.}
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm The light contained in is preferable light for germinating a straight solid from the disc-shaped body of Mozuku, and can promote the germination of the straight solid from the disc-shaped body of Mozuku.

Examples of the white light include light such as sunlight, a fluorescent lamp, and an incandescent lamp, and those containing the above-mentioned (A) light, (B) light, and (C) light in equal amounts are preferable. It does not have to be the amount of light, and it is sufficient that the light of (A), the light of (B), and the visible light of each wavelength contained in the light of (C) are mixed and appear white. The amount of white light is preferably 10 μmol / m ² / s or more, and the preferred range of the amount of light is 15 to 200 μmol / m ² / s, more preferably 25 to 100 μmol / m ² / s, still more preferably. It is 50 to 100 μmol / m ² / s.

Examples of the above-mentioned light (A) of 400 nm or more and less than 500 nm include blue LED (peak wavelength: 453 nm +/- 10 nm, wavelength range: 430 to 500 nm) when an LED is used as a light source. , Not limited to these.

Examples of the above-mentioned (B) light of 500 nm or more and less than 600 nm include light of a green LED (peak wavelength: 525 nm +/- 10 nm, wavelength range: 490 to 550 nm) when an LED is used as a light source. , Not limited to these.

Examples of the above (C) light of 600 nm or more and less than 700 nm include light of a red LED (peak wavelength: 641 nm +/- 10 nm, wavelength range: about 630 to 680 nm) when an LED is used as a light source. However, it is not limited to these.

As the light to be irradiated, the light of (A) and / or the light of (B), the light of (C), or the light of (A) and / or the light of (B) can be used. , Preferably the light of (A), more preferably blue light having a peak wavelength of 453 nm, and even more preferably blue LED light.

In the present invention, the amount of light when irradiating the plate-shaped body of mozuku with only the light of (A) and / or the light of (B) is 25 μmol / m ² / s or more. The preferable range of the amount of light varies depending on the type of light, but is preferably 25 to 200 μmol / m ² / s, more preferably 25 to 150 μmol / m ² / s, and further preferably 25 to 100 μmol / m ^2. / S, most preferably 25-80 μmol / m ² / s. If it is within the above range, it is preferable to germinate a straight solid from the disc-shaped body of mozuku, and further, it is preferable because germination is promoted.

The light sources in the wavelength ranges of the light (A), the light (B), and the light (C) are not particularly limited, and are, for example, sunlight, fluorescent lamps, incandescent lamps, sodium lamps, and LEDs (light emitting diodes). : Light emitting diode) -derived light and the like. Considering that it is easy to control the wavelength range of light, does not generate heat, and has a low running cost, LED-derived light is preferable.

One of the present inventions is the light and / or (B) of the following (A) in ^{which the amount of light is 25 μmol / m 2} / s or more, respectively, on the plate-shaped body of mozuku in the presence or absence of white light. It is a method of germinating a straight solid from a disc-shaped body of mozuku, which is characterized by irradiating only light.
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm The light of (A) and the light of (B) described above are as described above.

One of the present inventions is the light of (A) and the light of (B) below, in which the ^{total amount of light is 50 μmol / m 2} / s or more on the disk-shaped body of Mozuku in the presence or absence of white light. It is a method of germinating a straight solid from a disc-shaped body of Mozuku, which is characterized by irradiating with the light of the following (C) having a light amount of 35 μmol / m ^{2 / s or less.}
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm The light of (A) above, (B) The light of (C) and the light of (C) are as described above.

In the present invention, the disk-shaped body of Mozuku is irradiated with the light of (A) and the light of (B) and the light of (C) above, and the light of (A) and the light of (B) at that time are irradiated. amount of total combined is at 50μmol ^{/ m} 2 ^/ s or more, preferably 50~200μmol ^{/ m} 2 ^/ s, more preferably 50~150μmol ^{/ m} 2 ^/ s, more preferably 60~100μmol It is / m ² / s, most preferably 60 to 80 μmol / m ² / s.

Further, the amount of light (C) is not more than 35μmol ^{/ m} 2 ^/ s, preferably 25μmol ^{/ m} 2 ^/ s or less, more preferably 15μmol ^{/ m} 2 ^/ s or less, and most preferably 10 .mu.mol ^{/ m} 2 / It is less than or equal to s. The light of (C), especially the red light having a wavelength exceeding 600 nm, tends to prevent the germination of a straight solid from the disc-shaped body of mozuku, so it is possible to reduce the amount of light as described above. It is preferable for the straight solid to germinate from the disc-shaped body of mozuku.

The environment for irradiating the plate-shaped body of mozuku with light is not particularly limited as long as it is in the presence or absence of white light and the disc-shaped body of mozuku can grow. The conditions such as can be exemplified.
The above seawater is not particularly limited, but may be seawater collected directly from the sea, underground seawater collected underground near the coast or underground of the seabed (for example, the continental shelf), deep seawater collected from the deep sea, and the like. May be good. Further, it is also possible to use water having the same composition as seawater by adding mineral components (for example, salts such as calcium, potassium, sodium and magnesium) necessary for distilled water, tap water and the like.
The temperature of seawater is, for example, preferably about 10 to 30 ° C, more preferably 15 to 25 ° C, and most preferably 20 to 22 ° C, but is not limited thereto.

As the light irradiation time cycle, for example, the light-dark time cycle of one day is 5 to 16 hours in the light period and 19 to 8 hours in the dark period (the total of the light period and the dark period is 24 hours) in 24 hours. The same applies hereinafter), preferably 6 hours to 12 hours in the light period, 18 to 12 hours in the dark period, more preferably 6 hours to 10 hours in the light period, 18 hours to 14 hours in the dark period, and even more preferably light. The period is 6 hours to 8 hours, and the dark period is 18 hours to 16 hours, but the period is not limited to these.
As the number of light irradiation days, for example, the cycle of the light irradiation time may be performed for about 14 days or more, preferably for about 28 days or more, more preferably for about 35 days or more, and for about 42 days or more. Is more preferable, but the present invention is not limited to these.

The nutritional conditions of seawater are not particularly limited, but the concentration of nitrate nitrogen, which is a major nutrient component for seaweeds such as mozuku, is preferably 0.1 to 50 μmol / L, more preferably 0.2 to 10 μmol. / L, more preferably 0.5 to 10 μmol / L. The phosphate phosphorus concentration is, for example, preferably 0.01 to 5 μmol / L, more preferably 0.02 to 1 μmol / L, and more preferably 0.05 to 1 μmol / L. In general, the nitrate nitrogen concentration of seawater is usually 30 μmol / L or less, and the phosphoric acid phosphorus concentration is 3 μmol / L or less. Therefore, for example, a nutrient solution for seaweed culture should be added. Is also possible.
The salt concentration of seawater is not particularly limited, but for example, it is preferably 1.5 to 4.5% by mass, more preferably 2 to 3.5% by mass, more preferably 2.8 to 3.3% by mass, or the like. Can be mentioned.

The method of irradiating the disc-shaped body of Mozuku with light is not particularly limited as long as the disc-shaped body is irradiated with the above-mentioned light in the presence or absence of white light, but for example, the presence of white light. Under or in the absence of light, (1) a method of irradiating a mozuku disc floating in seawater with light, (2) a substrate to which the mozuku disc is attached is placed in seawater and placed on the disc. A method of irradiating light can be mentioned. The method (2) above is preferable in consideration of further growing the straight solids germinated from the discoid body and harvesting mozuku seaweed.

The substrate to which the disc-like body of Mozuku is attached can be obtained by a conventional method. For example, a zygote generated by joining a female gamete and a male gamete of Mozuku, or a zygote of Mozuku. It can be obtained by culturing with the above substrate in seawater containing neutral zoospores generated from the neutral compound spore, attaching gametes or neutral zoospores to the substrate, and growing them into a mozuku disc. ..

The substrate is not particularly limited as long as it is a material to which a zygote or a neutral zoospore easily adheres, and for example, a material such as polypropylene, a chemical fiber obtained by blending vinylon and polypropylene, or a palm yarn can be selected. Further, the shape of the substrate is not particularly limited, and examples thereof include a net-like shape, a curtain-like shape, and a state in which a thread is wound around a frame.

As a method of installing the substrate to which the plate-shaped body of mozuku is attached in seawater, a method of installing it in the sea where the waves are calm, a method of filling a container such as a water tank installed on land with seawater, and installing it in it. The method etc. can be mentioned. The substrate is not particularly limited, but is preferably installed at a position of about 0 to 2 m below the water surface, more preferably at a position of about 0.1 to 1.5 m below the water surface, and about 0.2 to about 0.2 to below the water surface. It is more preferable to install it at a position of 1 m.

Further, one of the present inventions is a method in which a straight solid is germinated from a plate-shaped body of mozuku under irradiation with white light, in which the irradiation of light of the following (C) is reduced by a material.
(C) Light in the wavelength range of 600 nm or more and less than 700 nm

The material capable of reducing the light irradiation of the above (C) is not particularly limited, and examples thereof include glass, wood (plant fiber, etc.), plastic, etc., and cost, durability, and availability. From such a viewpoint, for example, a color sheet (sheet-like, net-like, film-like, etc.) used for civil engineering or agriculture can be mentioned. As such a color sheet, for example, a color sheet having a thickness of about 0.07 to 0.11 mm can be used, but the color sheet is not limited thereto. The color of the color sheet is not particularly limited, but from the viewpoint of reducing the light of (C), blue, green and the like are preferably used, and blue is preferable. In the case of plastic, the material of the color sheet includes polyvinyl chloride, polyethylene, polypropylene, polyester, nylon and the like. Further, the color sheets may be used in layers, and usually 1 to 6 sheets, preferably 2 to 4 sheets are used, but the present invention is not limited thereto. The white light and the light of (C) are as described above.

Further, as a material capable of reducing the irradiation of the light of (C), in addition to the above, for example, a net-like, sheet-like, or film that selectively transmits the light of (A) and / or the light of (B). Examples thereof include materials having a shape such as a shape. For example, a net-like material that selectively transmits the light of (A) and / or the light of (B) can be preferably used, and for example, Dioneo Shade Sokai (trade name: manufactured by Dio Kasei Co., Ltd.) or special product. Examples include, but are not limited to, the net-like materials described in Kai 2017-55686. For example, in the form of several (2 to 4) layers of Dioneo Shade Soukai, sunlight, that is, light containing light (A), light (B), and light (C) to (A) The light and / or the light of (B) is selectively transmitted, that is, the light of (A) and / or the light of (B) has a large amount of light, and the disc-shaped body of Mozuku can be irradiated. Therefore, it is preferable.

Further, one of the present inventions is to enhance the light of the following (A) and / or the light of (B) in the method of germinating a straight solid from a plate-like body of mozuku under irradiation with white light, or the following ( C) This is a method of diminishing the irradiation of light.
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm (A) Light and / or (B) In order to enhance the light of the light source, for example, a method of increasing the amount of light emitted from the light source, a method of concentrating the light of the light source and / or white light on the disk-shaped body of the light source by using a reflector, the light source and the mozuku. There is a method of getting closer to the disc-shaped body. Further, in order to attenuate the irradiation of the light of (C), the above-mentioned material may be used, but it may not be used. For example, in addition to the method of reducing the amount of light emitted from the light source, when the substrate to which the plate-shaped body of mozuku is attached is placed in seawater, the light irradiation is attenuated by the seawater existing between the substrate and the light source. May be good. Further, a method of keeping a distance between the light source and the plate-shaped body of mozuku may be used. The white light, the light of (A), the light of (B) and the light of (C) are as described above.

Hereinafter, the present invention will be described with reference to Examples, but this merely describes the present invention and does not limit the present invention.

[Example 1]
(1) Preparation of disc-shaped body of mozuku A slide glass was put into sterilized seawater in which mozuku (Okinawa mozuku), which had been confirmed to form a neutral compound ascus and swim of a neutral zoospore, was aerated and cultured, and the water temperature was 25. A slide glass to which neutral zoospores were attached was obtained by culturing for 48 hours at ° C., a light amount of 50 μmol / m ² / s, a light period of 12 hours, and a dark period of 12 hours. A slide glass with neutral zoospores is placed in a petri dish filled with sterile seawater, and the conditions are such that the water temperature is 25 ° C., the amount of light is 50 μmol / m ² / s, and the daily irradiation time cycle is 12 hours in the light period and 12 hours in the dark period. The cells were cultured in 3 weeks to obtain mozuku discs.

(2) Light irradiation and culture of mozuku discs The obtained mozuku discs were filled with sterilized seawater (nitrate nitrogen concentration: 2 μmol / L, phosphate phosphorus concentration: 0.2 μmol / L). Five to ten pieces were placed in each well of the 6-well microplate and allowed to stand in a constant temperature bath (model: CN-40A manufactured by Mitsubishi Machine Engineering Co., Ltd.). It was cultured by irradiating it with light under the following conditions. In the mozuku disc, assimilation threads rise from the mozuku disc by culturing, and a part of the mozuku becomes a neutral compound ascus to generate neutral zoospores, and then the disc is formed again. The number of disc-shaped bodies increased by 100 to 200 times from the initial 5 to 10.

[Culture conditions and irradiation light conditions]
Culture temperature: set to 25 ° C.
Irradiation time cycle: In one day, light was continuously irradiated for 12 hours (light period), and the light was turned off for 12 hours to make a dark place (dark period).
Light to be irradiated: The following light was irradiated using a three-wave tunable LED irradiation device (model: 3LH-64 manufactured by Nippon Medical Instruments Co., Ltd.) as a light source.
(C) Light in a wavelength range of 600 nm or more and less than 700 nm (peak wavelength 641 nm). Hereinafter, it may be abbreviated as "R".
(B) Light in a wavelength range of 500 nm or more and less than 600 nm (peak wavelength 525 nm). Hereinafter, it may be abbreviated as "G".
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (peak wavelength 453 nm). Hereinafter, it may be abbreviated as "B".
White light (light containing equal amounts of light (A), light (B), and light (C)). Hereinafter, it may be abbreviated as "RGB".
Amount of light to be irradiated: The amount of light shown in Table 1 was irradiated.

(3) Observation method of direct three-dimensional germination from mozuku disc-like body Photographs were taken under a stereomicroscope 14 days, 21 days, 28 days, 35 days, and 42 days after the start of light irradiation, and 1 mm ² The number of discs inside (the total number of ungerminated discs and germinated discs) and germinated discs (assimilation threads gather from the central part of the disc to form a straight solid) The germination rate was calculated by the following formula.

(4) Evaluation Criteria The germination rate for each observation day obtained was symbolized and evaluated according to the following criteria. The results are shown in Table 1. The germination rate 14 days and 21 days after the observation date was 0% in all the test plots, and is not shown in Table 1. Further, test groups 1 to 7 in Table 1 are examples, and test groups 8 to 15 are comparative examples.
[Evaluation criteria]
Evaluation D: Germination rate is 0%
C evaluation: Germination rate exceeds 0% and less than 10% B evaluation: Germination rate is 10% or more and less than 20% A evaluation: Germination rate is 20% or more and less than 30% S evaluation: Germination rate is 30% or more , Less than 50% SS evaluation: When the germination rate was 50% or more and the evaluation was B or higher, a straight solid was germinated from the discoid body of Mozuku, and it was judged to be effective for germination. In particular, when the evaluation was A or higher, it was judged that a straight solid was germinated from the disc-shaped body of mozuku, and not only was it very effective for germination, but also a germination promoting effect was observed.

As is clear from the evaluation results on the 42nd day of observation, only the light of (A) (peak wavelength 453 nm: wavelength range of about 400 to 500 nm) was irradiated, and the amount of light was adjusted to ^{25 to 100 μmol / m 2 / s.} Then, it was evaluated as A or SS, and it was confirmed that the straight solid was germinated from the discoid body of Mozuku, which was very effective for germination, and the promotion of germination was also confirmed.
Only the light of (B) (peak wavelength 525 nm: wavelength range of about 500 to 600 nm) and the light of (A) (peak wavelength 453 nm: wavelength range of about 400 to 500 nm) are irradiated in combination, and the amount of light is 25 μmol / m ² /, respectively. In the test group adjusted to s, the evaluation was S, and it was confirmed that the straight solids germinated from the disc-shaped body of Mozuku, which was very effective for germination and promoted germination.
In the test group in which only the light of (B) (peak wavelength 525 nm: wavelength range of about 500 to 600 nm) was irradiated and the amount of light ^{was adjusted to 50 μmol / m 2} / s, the evaluation was A. It was confirmed that it was very effective for germination and promoted germination.
Light of (C) (peak wavelength 641 nm: wavelength range about 600 to 700 nm), light of (B) (peak wavelength 525 nm: wavelength range of about 500 to 600 nm) and light of (A) (peak wavelength 453 nm: wavelength range about 400). In the test group in which the light intensity ^{was adjusted to 33.3 μmol / m 2} / s by irradiating with ~ 500 nm), the evaluation was B, and the straight solid was germinated from the disc-shaped body of Mozuku, which was effective for germination.
In the test group in which only the light of (B) (peak wavelength 525 nm: wavelength range of about 500 to 600 nm) was irradiated and the amount of light ^{was adjusted to 100 μmol / m 2} / s, the evaluation was B. Was germinated and was effective for germination.

From the results of Example 1, when a large amount of the light of (A) (that is, the color of the blue "B" light) is irradiated, and / or the light of (C) (that is, the color of the red "R" light). We hypothesized that a straight solid would easily germinate from the disc-shaped body of Mozuku when the irradiation of the light was reduced, and further experiments were conducted.

[Example 2]
(1) Preparation of mozuku seed thread with mozuku discs attached Put Okinawa mozuku with neutral compound ascus and sterile seawater in a glass container (plum liquor bottle), water temperature 25 ° C, white fluorescent lamp (light intensity: 50 μmol) ^/ m 2 / ^s) the light period 12 hours, irradiated under the conditions of 12 hours dark for 30 days aeration culture, a neutral zoospores was confirmed to swimming.
A part of the culture net (composition: 70% nylon, 30% polyethylene) used for culturing mozuku seaweed is cut out in the sterilized seawater in which the neutral zookeeper is swimming. Was added, and aeration culture was carried out for 2 weeks under the same conditions as described above to obtain mozuku seed net yarn having a disc-like body attached to the surface of the net yarn. The surface of the net thread was visually confirmed to see if the disc-shaped body was attached.

(2) Light irradiation and culture of mozuku discs The obtained mozuku seed net yarn is cut to 1.5 to 2.0 cm, and the cut mozuku seed net yarn is sterilized in seawater (nitrate nitrogen concentration: 2 μmol). Put one in each 300 mL culture aeration flask (hereinafter, simply referred to as "flask") filled with / L and phosphoric acid phosphorus concentration: 0.2 μmol / L, and put this in a constant temperature bath (model: CN-). It was allowed to stand in 40A (manufactured by Mitsubishi Machine Engineering Co., Ltd.) and cultured by irradiating it with light under the following conditions (test groups 16 to 23).
Two flasks were installed in the same constant temperature bath, one was left as it was (test plots 16 to 19), and the other was a blue net (trade name: Dioneo Shade Sokai Dio Kasei). Covered with (manufactured by the company) (test plots 20-23). In addition, the flask covered with the blue net is closer to the light source installed on the upper part of the constant temperature bath than the flask not covered with the blue net so that the amount of light irradiated to each mozuku seed net thread is the same. It was installed on a hill.

[Culture conditions]
Culture temperature: set to 24 ° C.
Irradiation time cycle: Four constant temperature baths are used, and the light / dark time cycle of each constant temperature bath per day (during 24 hours) is 6, 8, 10, 12 hours in the light period (18, 16, 14, 12 in the dark period). It was set to have four stages of time).
Frequency of replacement of sterilized seawater: The sterilized seawater was replaced with new sterilized seawater once a day.

[Conditions of light to irradiate]
<<"Lightblue" light: No blue net (test plots 16-19) >>
Light to be irradiated: Using a three-wavelength variable LED irradiation device (model: 3LH-64 manufactured by Nippon Medical Instruments Co., Ltd.) as a light source, adjust the ratio of the following "R", "G", and "B" light in each flask. Each mozuku seed net thread was irradiated as "light blue" light.
"R": (C) Light in the wavelength range of 600 nm or more and less than 700 nm (peak wavelength 641 nm).
"G": (B) Light in the wavelength range of 500 nm or more and less than 600 nm (peak wavelength 525 nm).
"B": (A) Light in the wavelength range of 400 nm or more and less than 500 nm (peak wavelength 453 nm).
Amount of light to irradiate: Total amount of light 50 μmol / m ² / s
("R": 10.0 μmol / m ² / s,
"G": 10.0 μmol / m ² / s,
“B”: 30.0 μmol / m ² / s) of “light blue” light was irradiated.

<<"Blue" light: With blue net (test plots 20-23) >>
Light to irradiate: By covering the flask with a blue net and irradiating it with the same "light blue" light as in the test plots 16 to 19, each mozuku seed net thread in each flask is "blue". It was irradiated as the light of.
Amount of light irradiated (under blue net coating): Total amount of light 50 μmol / m ² / s
("R": 3.0 μmol / m ² / s,
"G": 6.0 μmol / m ² / s,
“B”: 41.0 μmol / m ² / s) of “blue” light was irradiated.

(3) Observation method and evaluation criteria for direct three-dimensional germination from mozuku discs 14 days and 36 days after the start of light irradiation, the mozuku seed yarn was taken out into a chalet, and an overall photograph of the mozuku seed net yarn was taken with a digital camera. The germination status was observed by counting the number of straight solids of about 1 mm or more sprouting from the disc-shaped body of Mozuku seed net thread. The counted number of straight steric numbers was evaluated by symbolizing the degree of germination according to the following evaluation criteria. The results are shown in Table 2 and FIG.
[Evaluation symbols and evaluation criteria]
×: The number of straight steric numbers that can be confirmed in the whole photo is 0 〇: The number of straight steric numbers that can be confirmed in the whole photo is 1 to 5 ◎: The number of straight steric numbers that can be confirmed in the whole photo is 6 to 15 ◎ ◎: Confirmed in the whole photo When the number of straight steric numbers that can be formed is 16 or more and the evaluation symbol is "○" or more, the straight steric numbers are germinated from the disc-shaped body of Mozuku, and it is judged that they are effective for germination. In particular, when the evaluation symbol was "◎◎" or higher, it was judged that a straight solid was germinated from the disc-shaped body of mozuku, which was not only very effective for germination but also had a germination promoting effect.

Further, FIG. 2 shows photographs of seed net yarns taken on the 14th day of culturing for the test plots 16 to 17 and the test plots 20 to 21, which had good results. The shorter the light period, the finer the disc-shaped bodies were gathered together (dark arrow), and there was also a part where the disc-shaped bodies were gathered together at a level that could be regarded as a straight solid (light-colored arrow). A photograph of the seed net thread taken on the 0th day of the test (before irradiation) is also shown in FIG.

From the results of Table 2 and FIG. 2, the mozuku disc-shaped body is irradiated with "light blue" and "blue" light, which contains a large amount of blue light, on the mozuku seed net thread to which the mozuku disc-shaped body is attached. I was able to germinate a straight solid from. In particular, the "blue" light transmitted through the blue net was much more effective for the germination of a straight solid than the "light blue" light, and further had an effect of promoting germination. Although the details are unknown, it was found that many straight-stereoscopic germination was observed in the mozuku seed net yarn, which was irradiated with a large amount of blue light and a small amount of irradiation with red light.
Regarding the light irradiation time, it was newly found that irradiation from 6 hours in the light period (18 hours in the dark period) to 8 hours in the light period (16 hours in the dark period) is particularly effective for germination of the straight solid.

Claims (4)

In the presence or absence of white light
The disk-shaped body of Mozuku, light amount below the light, and the light amount is less than 35 [mu] mol ^{/ m} 2 / s in light and / or the following (A) is 25 [mu] mol ^{/ m} 2 / s or more, respectively (B) (C) A method of germinating a straight solid from a disc-shaped body of a mozuku, which is characterized by irradiating with the light of.
(A) Light in the wavelength range of 400 nm or more and less than 500 nm (B) Light in the wavelength range of 500 nm or more and less than 600 nm (C) Light in the wavelength range of 600 nm or more and less than 700 nm The method for germinating a straight solid from a mozuku disc-shaped body according to claim 1, wherein the mozuku disc-shaped body is irradiated with only the light of (A) and / or the light of (B). The light of (A) and the light of (B) having a total amount of light of 50 μmol / m ^{2 / s or more on the plate-shaped body of mozuku,}
The method for germinating a straight solid from a mozuku disc-shaped body according to claim 1, wherein the light is irradiated with the light of (C) having a light amount of 35 μmol / m ^{2 / s or less.} The method for germinating a straight solid from a mozuku disc-shaped body according to any one of claims 1 to 3, wherein the light irradiating the mozuku disc-shaped body is light derived from an LED.

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