JP2020536491A5 - - Google Patents
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- Publication number
- JP2020536491A5 JP2020536491A5 JP2020509047A JP2020509047A JP2020536491A5 JP 2020536491 A5 JP2020536491 A5 JP 2020536491A5 JP 2020509047 A JP2020509047 A JP 2020509047A JP 2020509047 A JP2020509047 A JP 2020509047A JP 2020536491 A5 JP2020536491 A5 JP 2020536491A5
- Authority
- JP
- Japan
- Prior art keywords
- rbp
- oligonucleotide
- complex
- rna
- lna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 16
- 101710159080 Aconitate hydratase A Proteins 0.000 claims 10
- 101710159078 Aconitate hydratase B Proteins 0.000 claims 10
- 102000044126 RNA-Binding Proteins Human genes 0.000 claims 10
- 101710105008 RNA-binding protein Proteins 0.000 claims 10
- 108091034117 Oligonucleotide Proteins 0.000 claims 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 4
- 239000000523 sample Substances 0.000 claims 4
- 108091028664 Ribonucleotide Proteins 0.000 claims 3
- 239000012620 biological material Substances 0.000 claims 3
- 239000002336 ribonucleotide Substances 0.000 claims 3
- 125000002652 ribonucleotide group Chemical group 0.000 claims 3
- 239000000872 buffer Substances 0.000 claims 2
- 230000000295 complement effect Effects 0.000 claims 2
- KWGKDLIKAYFUFQ-UHFFFAOYSA-M lithium chloride Chemical compound [Li+].[Cl-] KWGKDLIKAYFUFQ-UHFFFAOYSA-M 0.000 claims 2
- 239000007787 solid Substances 0.000 claims 2
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 claims 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 claims 1
- 230000004570 RNA-binding Effects 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000011109 contamination Methods 0.000 claims 1
- 239000013068 control sample Substances 0.000 claims 1
- 238000004132 cross linking Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 230000029087 digestion Effects 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000012634 fragment Substances 0.000 claims 1
- YFVGRULMIQXYNE-UHFFFAOYSA-M lithium;dodecyl sulfate Chemical compound [Li+].CCCCCCCCCCCCOS([O-])(=O)=O YFVGRULMIQXYNE-UHFFFAOYSA-M 0.000 claims 1
- 239000006249 magnetic particle Substances 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 150000007523 nucleic acids Chemical class 0.000 claims 1
- 239000002773 nucleotide Substances 0.000 claims 1
- 125000003729 nucleotide group Chemical group 0.000 claims 1
- 210000000056 organ Anatomy 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 238000010833 quantitative mass spectrometry Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 238000010008 shearing Methods 0.000 claims 1
- 239000007790 solid phase Substances 0.000 claims 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP17186948.0 | 2017-08-18 | ||
| EP17186948 | 2017-08-18 | ||
| PCT/EP2018/072348 WO2019034783A1 (en) | 2017-08-18 | 2018-08-17 | IMPROVED RNA INTERACTOME CAPTURE (ERIC) |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020536491A JP2020536491A (ja) | 2020-12-17 |
| JP2020536491A5 true JP2020536491A5 (enExample) | 2021-09-09 |
| JP7313696B2 JP7313696B2 (ja) | 2023-07-25 |
Family
ID=59702542
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020509047A Active JP7313696B2 (ja) | 2017-08-18 | 2018-08-17 | 増強されたＲＮＡインタラクトーム捕捉（ｅＲＩＣ） |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US12117437B2 (enExample) |
| EP (1) | EP3669193B1 (enExample) |
| JP (1) | JP7313696B2 (enExample) |
| CN (1) | CN110998332B (enExample) |
| AU (1) | AU2018316551B2 (enExample) |
| CA (1) | CA3073297A1 (enExample) |
| DK (1) | DK3669193T3 (enExample) |
| ES (1) | ES2921301T3 (enExample) |
| HR (1) | HRP20220722T1 (enExample) |
| HU (1) | HUE059038T2 (enExample) |
| PL (1) | PL3669193T3 (enExample) |
| PT (1) | PT3669193T (enExample) |
| RS (1) | RS63423B1 (enExample) |
| WO (1) | WO2019034783A1 (enExample) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110205365B (zh) * | 2019-07-02 | 2023-07-25 | 中山大学孙逸仙纪念医院 | 一种高效研究rna相互作用组的高通量测序方法及其应用 |
| GB202101188D0 (en) * | 2021-01-28 | 2021-03-17 | Cambridge Entpr Ltd | Method |
| WO2024097262A1 (en) * | 2022-10-31 | 2024-05-10 | University Of Virginia Patent Foundation | Chemoproteomic capture of rna binding activity in living cells |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1999014226A2 (en) * | 1997-09-12 | 1999-03-25 | Exiqon A/S | Bi- and tri-cyclic nucleoside, nucleotide and oligonucleotide analogues |
| EP1706489B9 (en) * | 2003-12-23 | 2011-01-05 | Santaris Pharma A/S | Oligomeric compounds for the modulation of bcl-2 |
| DK2191016T3 (en) | 2007-08-20 | 2015-06-08 | Gen Hospital Corp | INSULATION OF PROTEIN FACTORS BINDING DIRECT OR INDIRECT WITH NUCLEIC ACIDS |
| US8748354B2 (en) | 2011-08-09 | 2014-06-10 | The Board Of Trustees Of The Leland Stanford Junior University | RNA interactome analysis |
| EP4502607A3 (en) | 2014-02-07 | 2025-05-07 | European Molecular Biology Laboratory | Proteomic sample preparation using paramagnetic beads |
| US20150376612A1 (en) | 2014-06-10 | 2015-12-31 | The General Hospital Corporation | CCCTC-Binding Factor (CTCF) RNA Interactome |
| EP3271460A4 (en) | 2015-03-17 | 2019-03-13 | The General Hospital Corporation | RNA INTERACTOM OF POLYCOMB REPRESSIVE COMPLEX 1 (PRC1) |
-
2018
- 2018-08-17 CA CA3073297A patent/CA3073297A1/en active Pending
- 2018-08-17 US US16/639,795 patent/US12117437B2/en active Active
- 2018-08-17 CN CN201880053251.2A patent/CN110998332B/zh active Active
- 2018-08-17 HR HRP20220722TT patent/HRP20220722T1/hr unknown
- 2018-08-17 JP JP2020509047A patent/JP7313696B2/ja active Active
- 2018-08-17 EP EP18756423.2A patent/EP3669193B1/en active Active
- 2018-08-17 PT PT187564232T patent/PT3669193T/pt unknown
- 2018-08-17 AU AU2018316551A patent/AU2018316551B2/en active Active
- 2018-08-17 WO PCT/EP2018/072348 patent/WO2019034783A1/en not_active Ceased
- 2018-08-17 RS RS20220632A patent/RS63423B1/sr unknown
- 2018-08-17 ES ES18756423T patent/ES2921301T3/es active Active
- 2018-08-17 PL PL18756423.2T patent/PL3669193T3/pl unknown
- 2018-08-17 HU HUE18756423A patent/HUE059038T2/hu unknown
- 2018-08-17 DK DK18756423.2T patent/DK3669193T3/da active
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