JP2020532291A5 - - Google Patents

Info

Publication number

JP2020532291A5

JP2020532291A5 JP2020502409A JP2020502409A JP2020532291A5 JP 2020532291 A5 JP2020532291 A5 JP 2020532291A5 JP 2020502409 A JP2020502409 A JP 2020502409A JP 2020502409 A JP2020502409 A JP 2020502409A JP 2020532291 A5 JP2020532291 A5 JP 2020532291A5

Authority

JP

Japan

Prior art keywords

cell

homology arm

donor polynucleotide

genomic dna

selectable marker

Prior art date

2018-07-18

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 210000004027 cell Anatomy 0.000 claims description 60
• 108091033319 polynucleotide Proteins 0.000 claims description 55
• 102000040430 polynucleotide Human genes 0.000 claims description 55
• 239000002157 polynucleotide Substances 0.000 claims description 55
• 239000003550 marker Substances 0.000 claims description 44
• 108020004414 DNA Proteins 0.000 claims description 39
• 108091033409 CRISPR Proteins 0.000 claims description 38
• 108020005004 Guide RNA Proteins 0.000 claims description 30
• 239000013598 vector Substances 0.000 claims description 18
• 230000001404 mediated effect Effects 0.000 claims description 15
• 230000005782 double-strand break Effects 0.000 claims description 12
• 108091027967 Small hairpin RNA Proteins 0.000 claims description 11
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• 230000034431 double-strand break repair via homologous recombination Effects 0.000 claims description 6
• 230000035772 mutation Effects 0.000 claims description 6
• 101710163270 Nuclease Proteins 0.000 claims description 5
• 239000002458 cell surface marker Substances 0.000 claims description 5
• 102100024222 B-lymphocyte antigen CD19 Human genes 0.000 claims description 4
• 101000980825 Homo sapiens B-lymphocyte antigen CD19 Proteins 0.000 claims description 4
• 108700008625 Reporter Genes Proteins 0.000 claims description 4
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• 239000007787 solid Substances 0.000 claims description 4
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• 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 claims description 2
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