JP2020529221A - 組み換えタンパク質発現のための融合タグ - Google Patents

組み換えタンパク質発現のための融合タグ Download PDF

Info

Publication number
JP2020529221A
JP2020529221A JP2020527858A JP2020527858A JP2020529221A JP 2020529221 A JP2020529221 A JP 2020529221A JP 2020527858 A JP2020527858 A JP 2020527858A JP 2020527858 A JP2020527858 A JP 2020527858A JP 2020529221 A JP2020529221 A JP 2020529221A
Authority
JP
Japan
Prior art keywords
fusion
deep
protein
fusion protein
tag
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2020527858A
Other languages
English (en)
Japanese (ja)
Other versions
JP2020529221A5 (OSRAM
Inventor
ヘクト，マイケル
ザージトスキー，シュロモ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of JP2020529221A publication Critical patent/JP2020529221A/ja
Publication of JP2020529221A5 publication Critical patent/JP2020529221A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2020527858A 2017-07-28 2018-07-27 組み換えタンパク質発現のための融合タグ Pending JP2020529221A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
US62/538,505 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS

Publications (2)

Publication Number Publication Date
JP2020529221A true JP2020529221A (ja) 2020-10-08
JP2020529221A5 JP2020529221A5 (OSRAM) 2021-09-02

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2020527858A Pending JP2020529221A (ja) 2017-07-28 2018-07-27 組み換えタンパク質発現のための融合タグ

Country Status (10)

Country Link
US (1) US12473340B2 (OSRAM)
EP (1) EP3658574A1 (OSRAM)
JP (1) JP2020529221A (OSRAM)
KR (1) KR20200037819A (OSRAM)
CN (1) CN111132996A (OSRAM)
AU (1) AU2018307960B2 (OSRAM)
CA (1) CA3071419A1 (OSRAM)
MX (1) MX2020001058A (OSRAM)
SG (1) SG11202000775SA (OSRAM)
WO (1) WO2019023616A1 (OSRAM)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111132996A (zh) 2017-07-28 2020-05-08 普林斯顿大学理事会 用于重组蛋白表达的融合标签
WO2020252266A1 (en) * 2019-06-14 2020-12-17 Mytide Therapeutics, Inc. A manufacturing process for peptide and protein production
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
WO2022155389A2 (en) * 2021-01-14 2022-07-21 Biocatalyst Llc Compositions and methods for peptide production
CN113388009B (zh) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用
KR102868986B1 (ko) * 2022-09-27 2025-10-15 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
EP2084179B1 (en) 2006-11-22 2013-05-22 Novo Nordisk A/S Method for making activated carboxypeptidases
US9388227B2 (en) 2012-03-29 2016-07-12 Biocon Limited Process of expressing a fully folded functional two chain insulin glargine
CN103031328B (zh) * 2012-11-22 2015-08-05 江南大学 利用一种融合标签提高外源蛋白质的表达量
US20160024169A1 (en) 2013-03-14 2016-01-28 Indiana University Research And Technology Corporation Insulin-incretin conjugates
SG11201600684XA (en) 2013-07-31 2016-02-26 Biogenomics Ltd Process for production of insulin and insulin analogues
US10202607B2 (en) * 2015-10-13 2019-02-12 The Research Foundation For The State University Of New York Cleavable fusion tag for protein overexpression and purification
CN111132996A (zh) 2017-07-28 2020-05-08 普林斯顿大学理事会 用于重组蛋白表达的融合标签

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
3 BIOTECH, vol. Vol. 6, Article number: 44, JPN5021000286, 4 February 2016 (2016-02-04), ISSN: 0004798931 *
BIOPHYSICAL CHEMISTRY, vol. 123, JPN6022023689, 29 April 2006 (2006-04-29), pages 102 - 112, ISSN: 0005012090 *
PROTEIN ENGINEERING, DESIGN & SELECTION, vol. 18, JPN7022002706, 22 April 2005 (2005-04-22), pages 201 - 207, ISSN: 0004798934 *
UNIVERSIDADE DO MINHO: DEVELOPMENT OF A NOVEL FUSION SYSTEM FOR RECOMBINANT PROTEIN PRODUCTION AND P, JPN6022023687, 31 May 2013 (2013-05-31), ISSN: 0004798932 *
信州大学機関リポジトリ, SELF-ASSEMBLING NANOSTRUCTURES CREATED FROM PROTEIN NANOBUILDING BLOCKS USIN, JPN6022023688, 25 April 2017 (2017-04-25), ISSN: 0005012088 *

Also Published As

Publication number Publication date
US20200216512A1 (en) 2020-07-09
CN111132996A (zh) 2020-05-08
WO2019023616A1 (en) 2019-01-31
KR20200037819A (ko) 2020-04-09
MX2020001058A (es) 2020-08-06
EP3658574A1 (en) 2020-06-03
AU2018307960A1 (en) 2020-02-27
CA3071419A1 (en) 2019-01-31
US12473340B2 (en) 2025-11-18
SG11202000775SA (en) 2020-02-27
AU2018307960B2 (en) 2023-03-23

Similar Documents

Publication Publication Date Title
JP2020529221A (ja) 組み換えタンパク質発現のための融合タグ
CN104053779B (zh) 断裂内含肽及其用途
KR101971021B1 (ko) 세포 투과성의 향상을 위한 개선된 거대분자 전달 도메인(aMTD) 서열, 이를 코딩하는 폴리뉴클레오타이드, 이를 포함하는 aMTD의 독특한 특징을 확인하는 방법, 이를 포함하는 aMTD 서열을 개발하는 방법
EP2109673B1 (en) Methods and compositions for enhanced protein expression and purification
US8119369B2 (en) Human SUMO-3 for enhancing protein expression
CA2159079C (en) Methods and dna expression systems for over-expression of proteins in host cells
CN106755042B (zh) 一种基于组合自剪切与蛋白支架的生物活性小肽制备方法
CN111073925B (zh) 一种基于无序蛋白偶联酶的高效多肽-多肽偶联系统和方法
CN101622269A (zh) 蛋白质在大肠杆菌中的表达
Abd Elhameed et al. Purification of proteins with native terminal sequences using a Ni (II)-cleavable C-terminal hexahistidine affinity tag
JP2005514028A (ja) 融合タンパク質
CN116496365B (zh) 一种提高重组蛋白表达效率的酸性表面助溶短肽标签
KR20230165291A (ko) 폴리펩티드의 발현 증가를 위한 구축물 및 방법
US7223742B2 (en) Enhanced solubility of recombinant proteins
CA2529282C (en) Recombinant igf expression systems
Greenshields et al. Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
US6632638B1 (en) Enhanced solubility of recombinant proteins using Uracil DNA glycosylase inhibitor
WO2008147859A2 (en) Self-assembled proteins and related methods and protein structures
WO2003040312A2 (en) Multifunctional green fluorescent protein and uses thereof
KR102185312B1 (ko) 목적 단백질의 가용화용 조성물 및 이의 용도
WO2022094146A1 (en) Methods of converting precursor proteins to mature proteins using kex2 proteases

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20210721

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20210721

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20220531

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20220614

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20220909

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20221111

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20221213

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20230314