JP2020509746A - 3dプリンティング及び3d細胞培養培地に使用するためのマイクロゲル粒子及び関連する組成物、システム、及び方法 - Google Patents
3dプリンティング及び3d細胞培養培地に使用するためのマイクロゲル粒子及び関連する組成物、システム、及び方法 Download PDFInfo
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- FQPSGWSUVKBHSU-UHFFFAOYSA-N methacrylamide Chemical compound CC(=C)C(N)=O FQPSGWSUVKBHSU-UHFFFAOYSA-N 0.000 description 1
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- ZQMHJBXHRFJKOT-UHFFFAOYSA-N methyl 2-[(1-methoxy-2-methyl-1-oxopropan-2-yl)diazenyl]-2-methylpropanoate Chemical compound COC(=O)C(C)(C)N=NC(C)(C)C(=O)OC ZQMHJBXHRFJKOT-UHFFFAOYSA-N 0.000 description 1
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- WVFLGSMUPMVNTQ-UHFFFAOYSA-N n-(2-hydroxyethyl)-2-[[1-(2-hydroxyethylamino)-2-methyl-1-oxopropan-2-yl]diazenyl]-2-methylpropanamide Chemical compound OCCNC(=O)C(C)(C)N=NC(C)(C)C(=O)NCCO WVFLGSMUPMVNTQ-UHFFFAOYSA-N 0.000 description 1
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- XFTALRAZSCGSKN-UHFFFAOYSA-M sodium;4-ethenylbenzenesulfonate Chemical compound [Na+].[O-]S(=O)(=O)C1=CC=C(C=C)C=C1 XFTALRAZSCGSKN-UHFFFAOYSA-M 0.000 description 1
- 239000012453 solvate Substances 0.000 description 1
- DYNABLLWIDHPDH-UHFFFAOYSA-N tert-butyl 3-ethylheptanoate Chemical compound CCCCC(CC)CC(=O)OC(C)(C)C DYNABLLWIDHPDH-UHFFFAOYSA-N 0.000 description 1
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- FZGFBJMPSHGTRQ-UHFFFAOYSA-M trimethyl(2-prop-2-enoyloxyethyl)azanium;chloride Chemical compound [Cl-].C[N+](C)(C)CCOC(=O)C=C FZGFBJMPSHGTRQ-UHFFFAOYSA-M 0.000 description 1
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- OEIXGLMQZVLOQX-UHFFFAOYSA-N trimethyl-[3-(prop-2-enoylamino)propyl]azanium;chloride Chemical compound [Cl-].C[N+](C)(C)CCCNC(=O)C=C OEIXGLMQZVLOQX-UHFFFAOYSA-N 0.000 description 1
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Abstract
Description
Claims (32)
- 3次元細胞培養培地に使用するための組成物であって、
該組成物が、
複数のマイクロゲル粒子であって、
複数のマイクロゲル粒子の各々が、架橋されたポリマーネットワークを含み、
該架橋されたポリマーネットワークが、
低電荷密度ポリマー分子、及び
架橋剤
を含む、マイクロゲル粒子
を含む、組成物。 - 低電荷密度ポリマー分子の各々が複数の電荷を帯びた基を含み、電荷を帯びた基間の平均間隔がビエルム長の1/4、1/2、1倍、1.5倍、又は2倍より大きい、請求項1に記載の組成物。
- 前記電荷を帯びた基が、負の電荷を帯びた基である、請求項2に記載の組成物。
- 前記低電荷密度ポリマーの各々が、第1次のモノマー単位及び第2次のモノマー単位を含み、第2次のモノマー単位が酸性モノマー由来であり、第2次のモノマー単位が電荷を帯びた基を含む、請求項2に記載の組成物。
- 第1及び第2次のモノマー単位の合計の60%未満が第2のモノマー単位である、請求項4に記載の組成物。
- 第1及び第2次のモノマー単位の合計の20%未満が第2のモノマー単位である、請求項4に記載の組成物。
- 第1次のモノマー単位がアクリルアミド由来である、請求項4に記載の組成物。
- 第2次のモノマー単位がメタクリル酸由来である、請求項4に記載の組成物。
- 前記架橋剤が、250g/molと10,000g/molとの間の平均分子量を有する、請求項1に記載の組成物。
- 前記架橋剤が、PEGDA又はMBAを含む、請求項1に記載の組成物。
- 前記架橋剤が親水性である、請求項1に記載の組成物。
- マイクロゲル粒子を形成する方法であって、
該方法が、
架橋剤、
第1のモノマー、
第2のモノマーであって、酸性モノマーである第2のモノマー、
開始剤、及び
溶媒、
を含む溶液を形成する工程、
該溶液中でポリマーの形成を開始する工程、及び
該溶液からポリマーを析出させる工程であって、ポリマーはマイクロゲル粒子を形成する工程、
を含む、方法。 - 前記溶媒がエタノールである、請求項12に記載の方法。
- 第1及び第2のモノマー分子の合計の60%未満が第2のモノマー分子である、請求項12に記載の方法。
- 第1及び第2のモノマー分子の合計の20%未満が第2のモノマー分子である、請求項12に記載の方法。
- 前記架橋剤が、250g/molと10,000g/molとの間の平均分子量を有する、請求項12に記載の方法。
- 前記架橋剤が、PEGDA又はMBAを含む、請求項12に記載の方法。
- 溶液中に存在する架橋剤に対する前記第1のモノマーのモル比が、10:1と200:1との間である、請求項12に記載の方法。
- 前記架橋剤が親水性である、請求項12に記載の方法。
- 前記第1のモノマーがアクリルアミドを含む、請求項12に記載の方法。
- 前記酸性モノマーがメタクリル酸を含む、請求項12に記載の方法。
- 前記開始の工程が、溶液を加熱することを含む、請求項12に記載の方法。
- 前記開始剤が熱的開始剤である、請求項22に記載の方法。
- 前記熱的開始剤がAIBNである、請求項23に記載の方法。
- 前記開始剤が、光開始剤又は触媒開始剤である、請求項12に記載の方法。
- 前記請求項のうちのいずれか一項に記載の複数のマイクロゲル粒子、及び
該マイクロゲル粒子が液体細胞培養培地で膨潤して顆粒上のゲルを形成する、液体細胞培養培地
を含む、3次元細胞培養培地。 - 前記3次元細胞培養培地が、5.5と6との間の、6.5と7.5との間の、又は4.5と8との間のpHを有する、請求項26に記載の3次元細胞培養培地。
- 前記3次元細胞培養培地が、およそ1Pa〜およそ10Pa、およそ1Pa〜およそ5Pa、又はおよそ1Pa〜およそ2Paの降伏応力を有する、請求項26に記載の3次元細胞培養培地。
- 3次元細胞培養培地を調製する方法であって、
該方法が、前記請求項のうちのいずれか一項に記載の複数のマイクロゲル粒子を液体細胞培養培地中で混合する工程
を含む、方法。 - 細胞を3次元細胞培養培地に配置する方法であって、
該方法が、
細胞を、液体細胞培養培地で膨潤させた、前記請求項のうちのいずれか一項に記載の複数のマイクロゲル粒子を含む顆粒状のゲル内に堆積させる工程
を含む、方法。 - タンパク質を合成する方法であって、
該方法が、
細胞を、液体細胞培養培地で膨潤させた、前記請求項のうちのいずれか一項に記載の複数のマイクロゲル粒子を含む顆粒状のゲルを含む容器内で培養する工程、及び
該培養した細胞により合成されたタンパク質を容器から抽出する工程
を含む、方法。 - 前記培養した細胞が膵島細胞であり、前記合成されたタンパク質がインシュリンである、請求項31に記載のタンパク質を合成する方法。
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WO2024144290A1 (ko) * | 2022-12-28 | 2024-07-04 | 주식회사 알데바 | 3d 프린팅용 하이드로겔 조성물 및 그로부터 제조된 3d 프린팅 하이드로겔 |
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