JP2020507312A - 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 - Google Patents

複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 Download PDF

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JP2020507312A
JP2020507312A JP2019539756A JP2019539756A JP2020507312A JP 2020507312 A JP2020507312 A JP 2020507312A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2019539756 A JP2019539756 A JP 2019539756A JP 2020507312 A JP2020507312 A JP 2020507312A
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dna
crispr
ctag
sequence
modular
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JP2020507312A5 (enExample
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チャイキンド，ブライアン
ロッサム，ヘンドリク マリヌス ヴァン
ロッサム，ヘンドリク マリヌス ヴァン
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Zymergen Inc
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    • C12N15/102—Mutagenizing nucleic acids
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3517—Marker; Tag

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2019539756A 2017-02-10 2018-02-09 複数の宿主用の複数のｄｎａコンストラクトのアセンブリ及び編集のためのモジュラーユニバーサルプラスミド設計戦略 Withdrawn JP2020507312A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762457493P 2017-02-10 2017-02-10
US62/457,493 2017-02-10
PCT/US2018/017573 WO2018148511A1 (en) 2017-02-10 2018-02-09 A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts

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JP2020507312A true JP2020507312A (ja) 2020-03-12
JP2020507312A5 JP2020507312A5 (enExample) 2021-03-25

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US (2) US11098305B2 (enExample)
EP (1) EP3580337A4 (enExample)
JP (1) JP2020507312A (enExample)
KR (1) KR20190116282A (enExample)
CN (1) CN110312797A (enExample)
CA (1) CA3049989A1 (enExample)
WO (1) WO2018148511A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3049989A1 (en) 2017-02-10 2018-08-16 Zymergen Inc. A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts
EP3821020A4 (en) 2018-08-15 2022-05-04 Zymergen Inc. APPLICATIONS OF CRISPRI IN HIGH-PERFORMANCE METABOLIC ENGINEERING
AU2020253531C1 (en) 2019-04-04 2022-06-16 Regeneron Pharmaceuticals, Inc. Methods for scarless introduction of targeted modifications into targeting vectors
US12497609B2 (en) * 2019-10-14 2025-12-16 Yonsei University Biohealth Technology Holdings. Inc. Protospacer adjacent motif sequence and method for modifying target nucleic acid in genome of cell by using same
KR102178813B1 (ko) 2019-10-18 2020-11-13 주식회사 다우진유전자연구소 Dna 구조체를 적용한 간이 키트
CN115052980B (zh) * 2019-11-18 2024-09-10 苏州齐禾生科生物科技有限公司 衍生自黄杆菌的基因编辑系统
US20230357796A1 (en) * 2019-11-27 2023-11-09 Danmarks Tekniske Universitet Constructs, compositions and methods thereof having improved genome editing efficiency and specificity
EP4114936A4 (en) 2020-03-03 2024-03-27 Codex Dna, Inc. METHOD FOR ASSEMBLY OF NUCLEIC ACIDS
CN113373130B (zh) * 2021-05-31 2023-12-22 复旦大学 Cas12蛋白、含有Cas12蛋白的基因编辑系统及应用
GB202117455D0 (en) * 2021-12-02 2022-01-19 Academisch Ziekenhuis Leiden Method of editing nucleic acid
WO2023194537A1 (en) * 2022-04-08 2023-10-12 Glaxosmithkline Intellectual Property Development Limited Novel processes for the production of polynucleotides including oligonucleotides
CN116284436B (zh) * 2022-11-25 2026-04-07 广州美格生物科技有限公司 嵌合体TsCas12a蛋白及其制备方法和应用
CN119432813A (zh) * 2023-12-29 2025-02-14 武汉艾迪晶生物科技有限公司 Cas蛋白、组合蛋白、核酸分子、核糖核蛋白复合物、重组载体、转基因细胞及其应用
WO2025165780A1 (en) * 2024-02-01 2025-08-07 The Board Of Trustees Of The Leland Stanford Junior University Combinatorial gene editing and screening

Family Cites Families (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994024143A1 (en) 1993-04-12 1994-10-27 Northwestern University Method of forming oligonucleotides
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
WO2000026381A2 (en) 1998-10-30 2000-05-11 Cornell Research Foundation, Inc. High fidelity thermostable ligase and uses thereof
KR20100133319A (ko) * 2009-06-11 2010-12-21 주식회사 툴젠 표적 특이적인 게놈의 재배열을 위한 표적 특이적 뉴클레아제 및 이의 용도
GB2481425A (en) * 2010-06-23 2011-12-28 Iti Scotland Ltd Method and device for assembling polynucleic acid sequences
LT3401400T (lt) 2012-05-25 2019-06-10 The Regents Of The University Of California Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
ES2576126T3 (es) 2012-12-12 2016-07-05 The Broad Institute, Inc. Modificación por tecnología genética y optimización de sistemas, métodos y composiciones enzimáticas mejorados para la manipulación de secuencias
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
CN114634950A (zh) 2012-12-12 2022-06-17 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
EP2931898B1 (en) 2012-12-12 2016-03-09 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
JP2016504026A (ja) 2012-12-12 2016-02-12 ザ・ブロード・インスティテュート・インコーポレイテッド 配列操作のための系、方法および最適化ガイド組成物のエンジニアリング
US10378027B2 (en) * 2013-03-15 2019-08-13 The General Hospital Corporation RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
US11414695B2 (en) 2013-05-29 2022-08-16 Agilent Technologies, Inc. Nucleic acid enrichment using Cas9
ES3029138T3 (en) 2013-06-05 2025-06-23 Univ Duke Rna-guided gene editing and gene regulation
WO2014204724A1 (en) * 2013-06-17 2014-12-24 The Broad Institute Inc. Delivery, engineering and optimization of tandem guide systems, methods and compositions for sequence manipulation
MX2015017312A (es) * 2013-06-17 2017-04-10 Broad Inst Inc Suministro y uso de composiciones, vectores y sistemas crispr-cas para la modificación dirigida y terapia hepáticas.
US10287590B2 (en) 2014-02-12 2019-05-14 Dna2.0, Inc. Methods for generating libraries with co-varying regions of polynuleotides for genome modification
RS60366B1 (sr) * 2014-06-23 2020-07-31 Regeneron Pharma Sastavljanje dnk posredovano nukleazom
US20180002706A1 (en) * 2014-12-30 2018-01-04 University Of South Florida Methods and compositions for cloning into large vectors
AU2016279077A1 (en) * 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
US9790490B2 (en) * 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
WO2016205759A1 (en) * 2015-06-18 2016-12-22 The Broad Institute Inc. Engineering and optimization of systems, methods, enzymes and guide scaffolds of cas9 orthologs and variants for sequence manipulation
US20190330659A1 (en) 2016-07-15 2019-10-31 Zymergen Inc. Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase
DK3491130T3 (da) * 2016-07-28 2022-10-24 Dsm Ip Assets Bv Samlingssystem til en eukaryot celle
CA3049989A1 (en) 2017-02-10 2018-08-16 Zymergen Inc. A modular universal plasmid design strategy for the assembly and editing of multiple dna constructs for multiple hosts
EP3821020A4 (en) * 2018-08-15 2022-05-04 Zymergen Inc. APPLICATIONS OF CRISPRI IN HIGH-PERFORMANCE METABOLIC ENGINEERING

Also Published As

Publication number Publication date
KR20190116282A (ko) 2019-10-14
CA3049989A1 (en) 2018-08-16
EP3580337A1 (en) 2019-12-18
US20210371856A1 (en) 2021-12-02
WO2018148511A1 (en) 2018-08-16
US11098305B2 (en) 2021-08-24
US20200123540A1 (en) 2020-04-23
EP3580337A4 (en) 2020-12-02
CN110312797A (zh) 2019-10-08

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