JP2020110178A - Utilizing method of cassava fermentation residue - Google Patents
Utilizing method of cassava fermentation residue Download PDFInfo
- Publication number
- JP2020110178A JP2020110178A JP2020052188A JP2020052188A JP2020110178A JP 2020110178 A JP2020110178 A JP 2020110178A JP 2020052188 A JP2020052188 A JP 2020052188A JP 2020052188 A JP2020052188 A JP 2020052188A JP 2020110178 A JP2020110178 A JP 2020110178A
- Authority
- JP
- Japan
- Prior art keywords
- cassava
- residue
- milk
- fermentation
- ethanol
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 104
- 238000000855 fermentation Methods 0.000 title claims abstract description 41
- 230000004151 fermentation Effects 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims description 8
- 241000658379 Manihot esculenta subsp. esculenta Species 0.000 title 1
- 240000003183 Manihot esculenta Species 0.000 claims abstract description 103
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 43
- 229920002472 Starch Polymers 0.000 claims abstract description 23
- 239000008107 starch Substances 0.000 claims abstract description 23
- 235000019698 starch Nutrition 0.000 claims abstract description 23
- 235000013336 milk Nutrition 0.000 claims abstract description 20
- 239000008267 milk Substances 0.000 claims abstract description 20
- 210000004080 milk Anatomy 0.000 claims abstract description 20
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 244000005700 microbiome Species 0.000 claims abstract description 12
- 210000001082 somatic cell Anatomy 0.000 claims abstract description 11
- 241001465754 Metazoa Species 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims description 11
- 241000235649 Kluyveromyces Species 0.000 claims description 2
- 239000002893 slag Substances 0.000 claims description 2
- 241000235070 Saccharomyces Species 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 description 27
- 229940088598 enzyme Drugs 0.000 description 12
- 241000283690 Bos taurus Species 0.000 description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 235000013365 dairy product Nutrition 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 5
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 5
- 240000008042 Zea mays Species 0.000 description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 5
- 235000005822 corn Nutrition 0.000 description 5
- 239000007788 liquid Substances 0.000 description 5
- 235000019750 Crude protein Nutrition 0.000 description 4
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 239000008101 lactose Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000238557 Decapoda Species 0.000 description 3
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 description 3
- 102100022624 Glucoamylase Human genes 0.000 description 3
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 description 3
- 108010059820 Polygalacturonase Proteins 0.000 description 3
- 244000253911 Saccharomyces fragilis Species 0.000 description 3
- 235000018368 Saccharomyces fragilis Nutrition 0.000 description 3
- 108010093305 exopolygalacturonase Proteins 0.000 description 3
- 229940031154 kluyveromyces marxianus Drugs 0.000 description 3
- 244000144972 livestock Species 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000004382 Amylase Substances 0.000 description 2
- 108010065511 Amylases Proteins 0.000 description 2
- 102000013142 Amylases Human genes 0.000 description 2
- 108010059892 Cellulase Proteins 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 102000004139 alpha-Amylases Human genes 0.000 description 2
- 108090000637 alpha-Amylases Proteins 0.000 description 2
- 229940024171 alpha-amylase Drugs 0.000 description 2
- 235000019418 amylase Nutrition 0.000 description 2
- 229940106157 cellulase Drugs 0.000 description 2
- 239000013256 coordination polymer Substances 0.000 description 2
- 230000000593 degrading effect Effects 0.000 description 2
- 230000006806 disease prevention Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 210000000265 leukocyte Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 235000008935 nutritious Nutrition 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 229920002261 Corn starch Polymers 0.000 description 1
- 241001235204 Fenneropenaeus merguiensis Species 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- 239000008120 corn starch Substances 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
- 235000012054 meals Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
本発明は、キャッサバ芋のでんぷんを抽出した後に残ったキャッサバ残渣を原料とし、これをエタノール発酵してエタノールを除去した後に残ったキャッサバ発酵残物を、動物用飼料として有効活用する方法に関するものである。 The present invention relates to a method for effectively utilizing the cassava fermentation residue remaining after the cassava residue remaining after extracting the starch of cassava potato as a raw material and ethanol-fermenting it to remove ethanol. is there.
キャッサバ芋を含め、でんぷんを含む作物は世界各地で重要な作物として栽培されており、でんぷんは、食用や工業原料として広く利用されている。例えば、トウモロコシでんぷんは、食用として利用されるほか、トウモロコシを原料としてエタノールを製造することが行われており、エタノールを抽出した残渣（トウモロコシＤＤＧＳ：トウモロコシ醸造粕）を家畜用飼料として利用することが知られている（引用文献１）。
キャッサバ芋の場合、根茎に含まれるでんぷんを食用にするほか、でんぷんを抽出した後のキャッサバの残渣を原料として、エタノールを製造することが検討されている（特許文献２）。
Starch-containing crops, including cassava, are cultivated as important crops around the world, and starch is widely used as an edible or industrial raw material. For example, corn starch is used not only as food but also as ethanol is produced from corn as a raw material, and the residue (corn DDGS: corn brewed lees) extracted from ethanol can be used as feed for livestock. It is known (cited document 1).
In the case of cassava, it is considered that starch contained in rhizome is edible and ethanol is produced using the residue of cassava after starch is extracted as a raw material (Patent Document 2).
特許文献１に記載されたＤＤＧＳは、でんぷんを含むトウモロコシを醸造して得た醸造粕を開示するもので、トウモロコシからでんぷんを抽出した残渣を原料としてエタノール発酵して得られたものを開示するものではない。特許文献２は、でんぷんを抽出したキャッサバ残渣をエタノール発酵する技術であって、エタノール分離後の残渣の利用を開示するものではない。すなわち、キャッサバにおいては、これまで、エタノール抽出後の残渣を利用することは行われていない。 The DDGS described in Patent Document 1 discloses a brewer's meal obtained by brewing corn containing starch, and discloses a product obtained by ethanol-fermenting a residue obtained by extracting starch from corn as a raw material. is not. Patent Document 2 is a technique for ethanol-fermenting a cassava residue obtained by extracting starch, and does not disclose use of the residue after ethanol separation. That is, in cassava, the residue after ethanol extraction has not been used so far.
本発明者らは、アミノ酸やビタミン等の栄養価がもともと乏しいでんぷん抽出後のキャッサバ残渣（以降、本願明細書では、単にキャッサバ残渣という。）を有効活用することを目的として、研究を進めたところ、キャッサバ残渣をでんぷん分解酵素と微生物とで発酵処理し、得られたキャッサバ残渣の発酵物からエタノールを除去して得られたキャッサバ発酵物の残渣（以後、「キャッサバ残渣ＤＤＧＳ」又は「キャッサバ発酵残物」という）には粗タンパク質含有量が豊富に含まれているだけでなく、キャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）を与えた乳牛のミルク中の白血球数が減少する知見を得て、キャッサバ発酵残物が家畜の病気等の予防に寄与することを見出し、本発明を完成するに至った。
すなわち、本発明の目的は、キャッサバ残渣を原料とした栄養価の高い動物又は魚類用飼料を提供することにある。
The present inventors have conducted research for the purpose of effectively utilizing cassava residue after starch extraction (hereinafter, simply referred to as cassava residue), which has originally poor nutritional values such as amino acids and vitamins. , The residue of the cassava fermentation product obtained by fermenting the cassava residue with a starch degrading enzyme and a microorganism and removing ethanol from the obtained fermented product of the cassava residue (hereinafter referred to as "cassava residue DDGS" or "cassava fermentation residue"). Not only is rich in crude protein content, but it was also found that the white blood cell count in milk of dairy cows fed cassava residue DDGS (cassava fermentation residue) decreased The present inventors have found that the residues contribute to the prevention of diseases of livestock, and have completed the present invention.
That is, an object of the present invention is to provide a highly nutritious animal or fish feed using cassava residue as a raw material.
本発明の動物用飼料であるキャッサバ残渣ＤＤＧＳは、でんぷん抽出後のキャッサバ残渣を原料とするものであり、キャッサバ残渣をでんぷん分解酵素と微生物とで発酵処理した後、当該アルコールを除去した後に得られるもので、好ましくは、発酵処理後、液体成分を除去して得られるものである。 The cassava residue DDGS that is an animal feed of the present invention is obtained by using cassava residue after starch extraction as a raw material, and is obtained after fermenting the cassava residue with a starch-degrading enzyme and a microorganism and then removing the alcohol. However, it is preferably obtained by removing the liquid component after the fermentation treatment.
本発明により、これまで利用価値のなかったキャッサバ残渣ＤＤＧＳを栄養価の高い飼料として利用することが可能となる。 According to the present invention, it is possible to use cassava residue DDGS, which has not been useful until now, as a highly nutritious feed.
以下、本発明の好ましい実施形態を説明する。
本発明で得られるキャッサバ残渣ＤＤＧＳは、キャッサバ残渣をでんぷん分解酵素とクリベロミセス属微生物とで発酵処理し、得られた発酵物から液体成分を除去して製造することができる。
Hereinafter, preferred embodiments of the present invention will be described.
The cassava residue DDGS obtained in the present invention can be produced by fermenting a cassava residue with a starch-degrading enzyme and a Kluyveromyces genus microorganism, and removing a liquid component from the obtained fermented product.
キャッサバ残渣とは、キャッサバ芋からでんぷんを搾取した滓であり、搾取後の水分含有量は、通常、７０重量％以上であるが、これに限定されるものではなく、水分含有量は、糖化発酵のためには、３０重量％以上であればよい。
また、乾燥したキャッサバ残渣を使用してもよい。乾燥キャッサバ残渣を使用する場合は、水を加え、前述の水分値に調整すればよく、また、乾燥キャッサバをでんぷん搾取後の高水分含有のキャッサバ残渣に混合して使用してもよい。
The cassava residue is a slag obtained by extracting starch from cassava potato, and the water content after extraction is usually 70% by weight or more, but the water content is not limited to this, and the water content is saccharification and fermentation. In order to satisfy the requirement, it may be 30% by weight or more.
Alternatively, dried cassava residue may be used. When the dried cassava residue is used, water may be added to adjust the water content to the above-mentioned value, or the dried cassava residue may be mixed with the high water content cassava residue after starch extraction.
キャッサバ残渣は、そのまま発酵処理してもよいが、キャッサバ残渣に残存するでんぷんを糊化するため、発酵処理の前に加熱処理することが好ましい。加熱条件は、６０℃以上で、５分以上行えばよい。加熱温度が６０℃以下の場合、キャッサバ残渣に残存するでんぷんの糊化効率が低くなることがある。
加熱時間は、特に限定されるものではなく、加熱温度を考慮して適宜定めればよい。
The cassava residue may be subjected to fermentation treatment as it is, but it is preferable to heat-treat it before the fermentation treatment in order to gelatinize the starch remaining in the cassava residue. The heating conditions may be 60° C. or higher and 5 minutes or longer. When the heating temperature is 60° C. or lower, the gelatinization efficiency of the starch remaining in the cassava residue may decrease.
The heating time is not particularly limited and may be appropriately determined in consideration of the heating temperature.
加熱処理後、キャッサバ残渣を冷却し、でんぷん分解酵素と微生物とで発酵処理する。でんぷん分解酵素による処理と発酵処理とは、別々に行ってもよいが、同時に行ってもよい。 After the heat treatment, the cassava residue is cooled and fermented with a starch-degrading enzyme and a microorganism. The treatment with a starch-degrading enzyme and the fermentation treatment may be performed separately, but may be performed simultaneously.
発酵処理に用いる微生物は、エタノール発酵に使用できる微生物であればよいが、酵母菌が好ましい。酵母菌として、具体的には、サッカロミセス属セレビシエ（Saccharomyces cerevisiae）又はクリベロミセス属マーキシアナス（Kluyveromyces marxianus）を用いることができるが、栄養価の観点及び発酵処理におけるコンタミネーションを防止する観点から、サッカロミセス属セレビシエよりも温度が高い条件下でも生育及び発酵が可能な耐熱性酵母であるクリベロミセス属マーキシアナスが好ましい。
発酵条件は、使用する酵素及び微生物に応じ、適宜定めてよい。
The microorganism used for the fermentation treatment may be any microorganism that can be used for ethanol fermentation, but yeast is preferable. As the yeast, specifically, Saccharomyces cerevisiae (Saccharomyces cerevisiae) or Kluyveromyces marxianus (Kluyveromyces marxianus) can be used. Cliveromyces genus Marxianas, which is a heat-resistant yeast capable of growing and fermenting even under conditions of higher temperature, is preferable.
Fermentation conditions may be appropriately determined depending on the enzyme and microorganism used.
使用するアミラーゼ分解酵素は、特に限定されるものではなく、でんぷんを分解する酵素であればよく、例えばαアミラーゼ、グルコアミラーゼを挙げることができる。
キャッサバ残渣の粘性を下げるために、でんぷん分解酵素以外に、ペクチナーゼ、セルラーゼを併用してもよい。
The amylase degrading enzyme to be used is not particularly limited and may be an enzyme that degrades starch, and examples thereof include α-amylase and glucoamylase.
In order to reduce the viscosity of the cassava residue, pectinase and cellulase may be used together with the starch-degrading enzyme.
α?アミラーゼは、キャッサバ残渣１ｇ当たり９×１０?５Ｕ以上６００Ｕ以下、好ましくは８×１０?３Ｕ以上０．６Ｕ以下で添加する。グルコアミラーゼは、キャッサバ残渣１ｇ当たり３×１０?４Ｕ以上２００Ｕ以下、好ましくは３×１０?２Ｕ以上０．２Ｕ以下で添加する。
セルラーゼ分解酵素を用いる場合、添加量は、キャッサバ残渣１ｇ当たり１×１０?４Ｕ以上１００Ｕ以下、で添加することが好ましい。
ペクチナーゼを用いる場合は、キャッサバ残渣１ｇ当たり１×１０?３Ｕ以上１０００Ｕ以下、特に１×１０?１Ｕ以上１Ｕ以下で添加することが好ましい。
alpha? amylase, 9 × 10? 5 U or 600U less per cassava residue 1g, preferably added below 8 × 10? 3 U or 0.6 U. Glucoamylase, cassava residue 1g per 3 × 10? 4 U or 200U less, preferably added below 3 × 10? 2 U or 0.2 U.
When a cellulase-degrading enzyme is used, it is preferably added at 1×10 4 U or more and 100 U or less per 1 g of cassava residue.
When pectinase is used, it is preferably added at 1×10 −3 U or more and 1000 U or less, particularly 1×10 −1 U or more and 1 U or less per 1 g of cassava residue.
得られた発酵物に含まれるエタノール等の液体成分は、発酵物を圧搾あるいは加熱して、発酵物から液体を除去する操作により行えばよい。 The liquid component such as ethanol contained in the obtained fermented product may be obtained by squeezing or heating the fermented product to remove the liquid from the fermented product.
キャッサバ残渣ＤＤＧＳは、粗タンパク質、ビタミン類を豊富に含むだけでなく、家畜の病気等の予防にも優れた効果を有するため、動物又は魚類用飼料として利用することができる。 The cassava residue DDGS not only contains abundant crude proteins and vitamins, but also has an excellent effect on prevention of diseases of livestock, and thus can be used as a feed for animals or fish.
以上述べたように、これまで用途のなかったキャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）を動物又は魚類用飼料として利用することができる。
以下、いくつかの実施例を挙げてさらに説明する。
As described above, the cassava residue DDGS (cassava fermentation residue), which has not been used so far, can be used as a feed for animals or fish.
Hereinafter, some examples will be further described.
キャッサバ残渣（含水分率７５％）１ｇを８０℃で１時間加熱処理した。
ついで、１．０ユニットのセルラーゼ及び１０ユニットのペクチナーゼ、５．５ユニットのα?アミラーゼ及び２．０ユニットのグルコアミラーゼを用い、加熱処理したキャッサバ残渣を３７℃以上で酵素処理した。
1 g of cassava residue (water content 75%) was heat-treated at 80° C. for 1 hour.
Then, the cassava residue which had been heat-treated was enzyme-treated at 37° C. or higher using 1.0 unit of cellulase, 10 units of pectinase, 5.5 units of α-amylase and 2.0 units of glucoamylase.
酵素処理後、クリベロミセス属マーキシアナス（Kluyveromyces marxianus）を加え、３０℃で発酵処理を行った。表１に、キャッサバ残渣とキャッサバ残渣の発酵物とに含まれる粗タンパク質、ビタミン類の含有量を示す。 After the enzyme treatment, Kluyveromyces marxianus was added and fermentation treatment was performed at 30°C. Table 1 shows the contents of crude proteins and vitamins contained in the cassava residue and the fermented product of the cassava residue.
表１に示すように、キャッサバ残渣の発酵物は、キャッサバ残渣に比べ、粗タンパク質量、ビタミン類を豊富に含み、栄養分が高いことがわかる。 As shown in Table 1, it can be seen that the fermented product of the cassava residue contains a large amount of crude protein and vitamins and has a high nutrition content as compared with the cassava residue.
得られたキャッサバ残渣の発酵物を圧搾して、エタノールを含む液体を除去してキャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）を製造した。
キャッサバ残渣、キャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）及び、でんぷん未抽出のキャッサバを原料としてエタノール発酵を行った後、エタノールを抽出して残った残渣（キャッサバＤＤＧＳ）（Sunshine Biotech International Co.,Ltd製商品）を餌として乳牛に６０日間与え、ミルクの生産量、ミルク中のラクトース含量及び、牛の健康状態を把握するため、ミルク中の白血球数と体細胞数とを調べた。
結果を図１から図３に示す。
図２のミルク中のラクトース含量は、ミルクの品質を示す指標であり、ラクトース含量は値が高いほど鮮度が高いことを示し、図３の体細胞数もミルクの品質を示す指標であるが、体細胞数が低いほど牛の健康状態が良い状況に保たれていることを意味する。
なお、グラフ横軸の記号「ＣＰ」はキャッサバ残渣、「ＦＣＰ」はキャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）を示す。「ＤＤＧＳ」は、でんぷん未抽出のキャッサバを原料としてエタノール発酵を行った後、エタノールを抽出して残った残渣（Sunshine Biotech International Co.,Ltd製商品）を意味する。
本発明のキャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）を与えた場合、キャッサバ残渣（ＣＰ）又はでんぷん未抽出のキャッサバを原料としてエタノール発酵を行った後、エタノールを抽出して残った残渣（Sunshine Biotech International Co.,Ltd製商品）（ＤＤＧＳ）を餌とした場合に比べ、ミルクの生産量が高く、鮮度がよく、乳牛の健康状態を良い状態に保つことができ、キャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）が飼料として優れたものであることがわかる。
The fermented product of the obtained cassava residue was squeezed to remove the liquid containing ethanol to produce cassava residue DDGS (cassava fermentation residue).
Cassava residue, cassava residue DDGS (cassava fermentation residue), and the residue (cassava DDGS) (Sunshine Biotech International Co., Ltd.) left after extracting ethanol and performing ethanol fermentation using cassava with no starch extracted as a raw material. (Product) was fed to dairy cows as food for 60 days, and the number of white blood cells and the number of somatic cells in the milk were examined in order to grasp the milk production amount, the lactose content in the milk, and the health condition of the cow.
The results are shown in FIGS.
The lactose content in milk in FIG. 2 is an index indicating the quality of milk, the higher the lactose content, the higher the freshness, and the somatic cell count in FIG. 3 is also an index indicating the quality of milk. The lower the number of somatic cells, the better the health of the cow.
The symbol "CP" on the horizontal axis of the graph represents cassava residue, and "FCP" represents cassava residue DDGS (cassava fermentation residue). “DDGS” means a residue (a product manufactured by Sunshine Biotech International Co., Ltd) that remains after the ethanol fermentation using cassava that has not been extracted with starch as the raw material.
When cassava residue DDGS (cassava fermentation residue) of the present invention was given, cassava residue (CP) or cassava unextracted with starch was used as a raw material to perform ethanol fermentation, and ethanol was then extracted to leave residue (Sunshine Biotech International Co., Ltd product) (DDGS) compared to when fed as a feed, milk production is high, freshness is good, the health of dairy cows can be kept in good condition, cassava residue DDGS (cassava fermentation residue) ) Is excellent as a feed.
バナメイエビの餌として、キャッサバ残渣ＤＤＧＳ又はキャッサバ残渣を配合した飼料を用い（飼料成分を表２に示す）、エビの稚魚７匹を１ロットとして、それぞれの飼料について４ロットを４週間養殖した。 As a banana prawn feed, a feed containing cassava residue DDGS or cassava residue was used (feed components are shown in Table 2), and 7 shrimp fry were used as 1 lot, and 4 lots of each feed were cultured for 4 weeks.
養殖後のエビの個体重量は、キャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）配合飼料を用いた場合に増加する傾向がみられたほか、生存率に大きな相違がみられた。
生存率は、キャッサバ残渣を配合した飼料で６４．３％であるのに対し、キャッサバ残渣ＤＤＧＳ（キャッサバ発酵残物）配合飼料では、７５．０％と高くなっており、エビの総重量もキャッサバ残渣配合飼料が７８．９２ｇに対し、キャッサバ残渣ＤＤＧＳ配合飼料では、１００．３ｇであった。
The individual weight of shrimp after cultivation tended to increase when the cassava residue DDGS (cassava fermentation residue)-containing feed was used, and there was a large difference in the survival rate.
The survival rate was 64.3% with the cassava residue-containing feed, whereas it was 75.0% with the cassava residue DDGS (cassava fermentation residue)-containing feed, and the total weight of the shrimp was also cassava. The residual mixed feed was 78.92 g, whereas the cassava residual DDGS mixed feed was 100.3 g.
Claims (5)
得られたエタノール発酵物からエタノールを除去して、乳中の体細胞数を減少するキャッサバ発酵残物を得て、
該キャッサバ発酵残物を動物用飼料として用いることを特徴とする、キャッサバ発酵残物の利用方法。 The cassava residue, which is a slag after extracting starch from cassava, is ethanol-fermented with a starch-degrading enzyme and a microorganism,
By removing ethanol from the obtained ethanol fermentation product, to obtain a cassava fermentation residue that reduces the number of somatic cells in milk,
A method for utilizing a cassava fermentation residue, which comprises using the cassava fermentation residue as an animal feed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020052188A JP6887631B2 (en) | 2020-03-24 | 2020-03-24 | How to use cassava fermented residue |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020052188A JP6887631B2 (en) | 2020-03-24 | 2020-03-24 | How to use cassava fermented residue |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015156515A Division JP2017035005A (en) | 2015-08-06 | 2015-08-06 | Method for producing animal or fish feed and animal or fish feed |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020110178A true JP2020110178A (en) | 2020-07-27 |
| JP6887631B2 JP6887631B2 (en) | 2021-06-16 |
Family
ID=71666022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020052188A Active JP6887631B2 (en) | 2020-03-24 | 2020-03-24 | How to use cassava fermented residue |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP6887631B2 (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001309751A (en) * | 2000-05-02 | 2001-11-06 | Ajinomoto Co Inc | Additive for feed |
| US20100196537A1 (en) * | 2007-03-14 | 2010-08-05 | Danisco Us, Inc., Genencor Division | Production of ethanol from barley and ddgs containing reduced beta-glucan and phytic acid |
| JP2011030504A (en) * | 2009-07-31 | 2011-02-17 | Tokyo Univ Of Agriculture | Method for producing ethanol using immobilizing carrier |
| JP2011512873A (en) * | 2008-03-11 | 2011-04-28 | ダニスコ・ユーエス・インク | Glucoamylase and Butiauxella phytase in saccharification |
| JP2011217639A (en) * | 2010-04-06 | 2011-11-04 | Tokyo Univ Of Agriculture | Fermentation system utilizing fermentation bag and method for producing ethanol |
| JP2014014337A (en) * | 2012-07-10 | 2014-01-30 | Idemitsu Kosan Co Ltd | Treating method of cassava pulp |
| JP2014212776A (en) * | 2013-04-30 | 2014-11-17 | 出光興産株式会社 | Yeast culture method |
-
2020
- 2020-03-24 JP JP2020052188A patent/JP6887631B2/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001309751A (en) * | 2000-05-02 | 2001-11-06 | Ajinomoto Co Inc | Additive for feed |
| US20100196537A1 (en) * | 2007-03-14 | 2010-08-05 | Danisco Us, Inc., Genencor Division | Production of ethanol from barley and ddgs containing reduced beta-glucan and phytic acid |
| JP2011512873A (en) * | 2008-03-11 | 2011-04-28 | ダニスコ・ユーエス・インク | Glucoamylase and Butiauxella phytase in saccharification |
| JP2011030504A (en) * | 2009-07-31 | 2011-02-17 | Tokyo Univ Of Agriculture | Method for producing ethanol using immobilizing carrier |
| JP2011217639A (en) * | 2010-04-06 | 2011-11-04 | Tokyo Univ Of Agriculture | Fermentation system utilizing fermentation bag and method for producing ethanol |
| JP2014014337A (en) * | 2012-07-10 | 2014-01-30 | Idemitsu Kosan Co Ltd | Treating method of cassava pulp |
| JP2014212776A (en) * | 2013-04-30 | 2014-11-17 | 出光興産株式会社 | Yeast culture method |
Non-Patent Citations (1)
| Title |
|---|
| FLAVIO DIAS MAYER: "Conversion of cassava starch to ethanol and a byproduct under different hydrolysis conditions", STARCH, vol. 67, JPN6021015435, July 2015 (2015-07-01), pages 620 - 628, ISSN: 0004492902 * |
Also Published As
| Publication number | Publication date |
|---|---|
| JP6887631B2 (en) | 2021-06-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104970252B (en) | A kind of fermentation liquid piglet feed and preparation method thereof | |
| CN106497720B (en) | The deep working method of " four slags " in Kiwi berry complete utilization | |
| CN109247447B (en) | Yeast hydrolysate for replacing plasma protein powder to produce milk replacer and preparation method thereof | |
| CN104472879A (en) | Tea residue feed additive and preparation method thereof | |
| CN102823726A (en) | Biological fermentation method capable of improving protein content of cottonseed meal and removing toxicity | |
| JP5412035B2 (en) | Livestock feed and feed additives | |
| CN107937311B (en) | Streptococcus thermophilus for high yield of gamma-aminobutyric acid, preservation and culture method and method for preparing fermented milk by using streptococcus thermophilus | |
| CN112980646B (en) | Kefir source composite probiotic fermented pear juice and oat viable bacteria vinegar drink and preparation method thereof | |
| CN103907758A (en) | Preparation method of feeding fermentative bean sprout feed | |
| CN109198167A (en) | Yeast for animal feeds hydrolysate and its preparation method and application | |
| CN102726600A (en) | Technique for processing protein feed by insects | |
| CN109043223A (en) | A kind of preparation method of bioactivity environmental-protection aquatic product fodder | |
| JP2017035005A (en) | Method for producing animal or fish feed and animal or fish feed | |
| CN110616122A (en) | Cardamine hupingshanensis selenium-rich yellow wine and production method thereof | |
| CN104782906A (en) | Fermented feed for aquaculture | |
| CN108813101A (en) | A kind of fermentation process improving dregs of beans water-solubility protein | |
| CN105028926A (en) | Piglet edema prevention pig feed and preparation method thereof | |
| CN113528271A (en) | Yellow rice wine and preparation method thereof | |
| JP6887631B2 (en) | How to use cassava fermented residue | |
| CN111183954A (en) | Feeding method for fattening beef cattle | |
| CN102676340A (en) | Enzyme and leaven joint brewing method for papermulberry fruit red wine | |
| CN104489263A (en) | Broken-wall saccharomyces cerevisiae culture with bean pulp as base material and preparation method of culture | |
| CN107410701A (en) | A kind of method of sweet sorghum stalk fermented feed and application thereof | |
| CN105028896A (en) | Method for preparing sea-buckthorn biological fodder through compound microbial flora fermentation | |
| CN107467421A (en) | A kind of special perfect compound feed of crucian parent population |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200324 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A821 Effective date: 20200324 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20210427 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20210507 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 6887631 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |