JP2020089354A - Apparatus and method for producing microorganism strain - Google Patents
Apparatus and method for producing microorganism strain Download PDFInfo
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract
Description
本発明は、微生物菌株製造の技術分野に関わり、且つ一種の微生物菌株の製造装置および方法を公開した。 The present invention relates to the technical field of microbial strain production, and has disclosed a kind of microbial strain production apparatus and method.
土壤修復および生態系有機肥料に関する微生物菌株の製造、培養には、微生物菌株を基質と混合して培養し製造する必要があり、微生物菌株と顆粒基質とを混合することを必要とするが、顆粒基質では、微生物菌株と効果的に混合して微生物菌株の生長や発酵を進めるために、一般的に水が添加される。但し、水を添加した顆粒基質は回転混合時に大顆粒となりやすく、その結果、微生物菌株が大顆粒となった基質と均一に混合できなくなり、微生物菌株が栄養基質の栄養素を効果的に吸収できないこととなる。微生物菌株と基質を混合する一部の従来の装置は、ただ長時間にわたって撹拌するだけであるため、回転撹拌すると同時に大顆粒基質を効果的に破砕して混合する目的を達成できないため、微生物菌株の顆粒基質における混合効果を低下させて、顆粒基質の利用率を下げ、したがって、顆粒基質と微生物菌株の有効混合率を高めることができる微生物菌株の製造装置が期待される。 For the production and cultivation of microbial strains related to soil restoration and ecological organic fertilizer, it is necessary to mix and culture the microbial strain with a substrate, and it is necessary to mix the microbial strain with a granular substrate, but Water is generally added to the substrate in order to effectively mix with the microbial strain to promote growth and fermentation of the microbial strain. However, the granular substrate to which water is added tends to become large granules during rotary mixing, and as a result, the microbial strain cannot mix uniformly with the substrate that has become large granules, and the microbial strain cannot effectively absorb the nutrients of the nutrient substrate. Becomes Some conventional devices for mixing microbial strains and substrates only agitate for a long time, so it is not possible to achieve the purpose of effectively crushing and mixing large granule substrates at the same time with rotating agitation. An apparatus for producing a microbial strain is expected which can lower the mixing effect of the above-mentioned granular substrate to reduce the utilization rate of the granular substrate and thus increase the effective mixing ratio of the granular substrate and the microbial strain.
微生物菌株を製造するときに顆粒基質と効果的に混合できないという問題を解決する。 It solves the problem of not being able to mix effectively with a granular substrate when producing a microbial strain.
本体、軸受ホルダー、内筒体、仕込み口、大歯車、トルクモーター、噛合歯車、サーボモーター、トルク研磨ロッド、雄ネジ、ボールナット、２つのロケートリング、駆動モーター、トルク回転軸、アダプタプレート、シュート、補強軸受、スライダーブロック、弾性撹拌片、２つの内部押圧機構、隙間遮断機構、コントロールパネル、弾性ゴムスリーブおよびシールリングを含む微生物菌株の製造装置であって、前記軸受ホルダーはボルトで本体の内側の底部に固定して取り付けられ、本体の正面に開閉ドアが設置され、内筒体は本体の内側に設置され、軸受ホルダーの軸受内輪を貫通する。仕込み口は内筒体の左側に設置され、かつ本体の左側の内壁を貫通し、大歯車は内筒体に挿通され、トルクモーターは固定台を介して本体の内側に固定して取り付けられ、噛合歯車は大歯車の歯と噛み合って、かつトルクモーターの出力軸に套設され、サーボモーターはボルトにより本体の内側の最上部に固定して取り付けられ、サーボモーターの出力軸がカップリングを介してトルク研磨ロッドに接続され、トルク研磨ロッドの末端が軸受ベースを介して本体の内側の底部に移動可能に接続され、雄ネジはトルク研磨ロッドの円弧状側面に形成されており、ボールナットはトルク研磨ロッドの雄ネジ部分に螺合され、２つのロケートリングはそれぞれにトルク研磨ロッドの雄ネジ部分の両端に挿通され、駆動モーターはボールナットの左側面に固定して取り付けられ、駆動モーターの出力軸がカップリングを介してトルク回転軸に接続され、アダプタプレートは本体の内側の最上部と底部の間に固定して取り付けられ、シュートはアダプタプレートの側面に開けられ、スライダーブロックはシュートの内側に移動可能に接続され、補強軸受はスライダーブロックの内側に固定して取り付けられ、弾性ゴムスリーブは内筒体の右端面の貫通孔の内側に固定して取り付けられ、シールリングは弾性ゴムスリーブの内側に固定して取り付けられ、トルク回転軸の駆動モーターから離れた一端が補強軸受とシールリングの内側を貫通して内筒体の内側まで延伸しており、弾性撹拌片はトルク回転軸の内筒体の内側に位置する部分に均等に取り付けられ、２つの内部押圧機構はいずれも横方向に内筒体の内側に取り付けられ、隙間遮断機構は横方向に内筒体の内側に取り付けられ、コントロールパネルは本体の左側に設置される。 Main body, bearing holder, inner cylinder, charging port, large gear, torque motor, meshing gear, servo motor, torque polishing rod, male screw, ball nut, two locate rings, drive motor, torque rotation shaft, adapter plate, chute An apparatus for producing a microbial strain, comprising: a reinforcing bearing, a slider block, an elastic stirring piece, two internal pressing mechanisms, a gap blocking mechanism, a control panel, an elastic rubber sleeve and a seal ring, wherein the bearing holder is a bolt inside the main body. Is fixedly attached to the bottom of the main body, an opening/closing door is installed in front of the main body, the inner cylinder is installed inside the main body, and penetrates the bearing inner ring of the bearing holder. The charging port is installed on the left side of the inner cylinder, and penetrates the inner wall on the left side of the main body, the large gear is inserted into the inner cylinder, and the torque motor is fixedly attached to the inside of the main body via a fixing base, The meshing gear meshes with the teeth of the large gear and is mounted on the output shaft of the torque motor.The servo motor is fixedly attached to the innermost top of the main body by bolts, and the output shaft of the servo motor is connected via a coupling. Is connected to the torque polishing rod, the end of the torque polishing rod is movably connected to the inner bottom of the main body via the bearing base, the male screw is formed on the arc-shaped side surface of the torque polishing rod, and the ball nut is The two locate rings are screwed into the male thread of the torque polishing rod, and the two locate rings are inserted into both ends of the male thread of the torque polishing rod. The drive motor is fixedly attached to the left side of the ball nut. The output shaft is connected to the torque rotation shaft via a coupling, the adapter plate is fixedly mounted between the top and bottom inside the body, the chute is opened on the side of the adapter plate, and the slider block is the chute of the chute. It is movably connected to the inside, the reinforcing bearing is fixedly mounted inside the slider block, the elastic rubber sleeve is fixed inside the through hole of the right end surface of the inner cylinder, and the seal ring is the elastic rubber sleeve. It is fixedly installed inside the shaft, and one end of the torque rotating shaft away from the drive motor penetrates the inside of the reinforcing bearing and the seal ring and extends to the inside of the inner cylinder. The two internal pressing mechanisms are laterally mounted inside the inner tubular body, and the gap blocking mechanism is laterally mounted inside the inner tubular body. , The control panel is installed on the left side of the main body.
前記弾性撹拌片のトルク回転軸から離れた側辺に保護シートが粘着されている。 A protective sheet is adhered to the side of the elastic stirring piece that is remote from the torque rotation axis.
前記２つの内部押圧機構は、２つのアーチ状エッジエアバッグ、2本の導気管および２つの開閉バルブを含み、２つのアーチ状エッジエアバッグがそれぞれ内筒体の内側壁に粘着され、2本の導気管がそれぞれ２つのアーチ状エッジエアバッグの導気口に設置され、2本の導気管がそれぞれに内筒体の対応内側壁を貫通して本体のキャビティまで伸びており、２つの開閉バルブがそれぞれ2本の導気管に取り付けられる。 The two internal pressing mechanisms include two arched edge airbags, two air guide tubes and two opening/closing valves, and the two arched edge airbags are respectively adhered to the inner wall of the inner cylinder to form two Each of the two air guide pipes is installed at the air inlet of the two arched edge airbags, and each of the two air guide pipes extends through the corresponding inner wall of the inner cylinder to the cavity of the main body, and two open/close Valves are attached to each of the two conduits.
前記隙間遮断機構は、横方向中空バー、空気注入管、排気弁、接続ベース、遮断板、取付スロットおよび遮断エアバッグを含む。内筒体の内側壁に横方向バースロットが開けられ、横方向中空バーが横方向バースロットを介して横方向に内筒体の内側壁に取り付けられ、空気注入管が横方向中空バーの導気口に取り付けられ、空気注入管が内筒体の対応内側壁を貫通し、かつ排気弁に接続される。接続ベースが横方向中空バーの通気孔に固定して取り付けられ、接続ベースの底部における導気孔が対応した通気孔に連通し、遮断板が接続ベースに固定して接続され、且つ遮断板の接続ベースに接続された側面が吸気口を通して接続ベースの通気孔と互いに連通しており、取付スロットが遮断板の左右側面に対称的に開けられ、遮断エアバッグが取付スロットの内側辺に粘着され、遮断エアバッグの導気分岐管が遮断板の吸気口に固定して接続され、遮断エアバッグの外側辺が取付スロットの内側から伸びて、対応した弾性撹拌片の側辺と接触する。 The gap blocking mechanism includes a horizontal hollow bar, an air injection pipe, an exhaust valve, a connection base, a blocking plate, a mounting slot and a blocking airbag. A horizontal bar slot is opened in the inner wall of the inner cylinder, a horizontal hollow bar is laterally attached to the inner wall of the inner cylinder through the horizontal bar slot, and an air injection pipe is installed in the horizontal hollow bar. Attached to the air vent, an air injection pipe extends through the corresponding inner wall of the inner cylinder and is connected to the exhaust valve. The connection base is fixedly attached to the ventilation hole of the horizontal hollow bar, the air guide hole at the bottom of the connection base communicates with the corresponding ventilation hole, the blocking plate is fixedly connected to the connection base, and the connection of the blocking plate The side surface connected to the base communicates with the ventilation hole of the connection base through the intake port, the mounting slots are symmetrically opened on the left and right side surfaces of the blocking plate, and the blocking airbag is adhered to the inner side of the mounting slot. The air guide branch pipe of the shutoff airbag is fixedly connected to the intake port of the shutoff plate, and the outer side of the shutoff airbag extends from the inside of the mounting slot and contacts the corresponding side of the elastic stirring piece.
厳選される前記補強軸受は、内筒体の真右側に位置し、トルク回転軸とシールリングは中間ばめの嵌め合い関係を有し、弾性ゴムスリーブの初期状態が圧縮状態である。 The carefully selected reinforcing bearing is located on the right side of the inner cylindrical body, the torque rotary shaft and the seal ring have a fitting relationship of an intermediate fit, and the elastic rubber sleeve is in a compressed state in an initial state.
厳選される２つの前記アーチ状エッジエアバッグは、材質として軟質耐摩耗性ゴムを使い、アーチ状エッジエアバッグの側壁の厚みが６−８ミリメートルであり、アーチ状エッジエアバッグの内筒体と接触する側辺と内筒体は粘着により接続されている。 The two arched edge airbags that are carefully selected use soft wear-resistant rubber as a material, and the thickness of the side wall of the arched edge airbag is 6-8 mm. The contacting side and the inner cylinder are connected by adhesive.
厳選される前記コントロールパネルは、駆動モーター、トルクモーターおよびサーボモーターに電気的に接続され、コントロールパネルは、駆動モーター、トルクモーターおよびサーボモーターの起動・停止および作動状態を制御することができ、サーボモーターとして、回転数１０−１５ｒ／ｍｉｎの低速正逆回転モーターが使用される。 The carefully selected control panel is electrically connected to the drive motor, the torque motor and the servo motor, and the control panel can control the start/stop and the operating state of the drive motor, the torque motor and the servo motor. As the motor, a low speed forward/reverse rotation motor having a rotation speed of 10-15 r/min is used.
厳選される前記弾性撹拌片は、弾性プラスチック材を使い、断面形状が円弧状であり、弾性撹拌片と内筒体の内側壁との間に隙間を有し、弾性撹拌片は、トルク回転軸から内筒体の内側壁の側辺にかけて厚みが薄くなり、保護シートは弾性撹拌片の内筒体に近い側辺全体を粘着して包み、軟質ゴム材料を使うものである。 The elastic stirring piece that is carefully selected is made of an elastic plastic material, has a circular arc-shaped cross section, and has a gap between the elastic stirring piece and the inner side wall of the inner cylindrical body. To the side of the inner wall of the inner cylindrical body, the protective sheet is made of a soft rubber material by adhesively wrapping the entire side of the elastic stirring piece near the inner cylindrical body.
厳選される前記遮断板は、断面形状がアーチ状で、弾性プラスチック材で製造され、内筒体の内側壁に近い一側から内筒体の内側の一側にかけて厚みが次第に薄くなる。 The carefully selected blocking plate has an arch-shaped cross section and is made of an elastic plastic material, and the thickness gradually decreases from one side close to the inner wall of the inner cylindrical body to one inner side of the inner cylindrical body.
厳選される前記遮断エアバッグは、軟質ゴム材料で製造され、遮断板は接続ベースを介して等距離で横方向中空バーにおいて横方向に配置され、遮断板と弾性撹拌片が交互に配置されて、遮断板とそれと隣接する弾性撹拌片との間に隙間を有する。 The carefully selected shut-off airbag is made of a soft rubber material, the shut-off plates are arranged laterally in the horizontal hollow bar at equal distances through the connection base, and the shut-off plates and the elastic stirring pieces are alternately arranged. There is a gap between the blocking plate and the elastic stirring piece adjacent thereto.
微生物菌株の製造方法であって、：
（1）、基質と製造すべき微生物菌株を仕込み口から内筒体のキャビティに投入して、基質の投入量に応じて内筒体のキャビティにおける基質と微生物菌株の混合スペースを調整し、本体の開閉ドアを開いて、開閉バルブを開き、2本の導気管を給気装置に外付けしてアーチ状エッジエアバッグのキャビティに気体を注入し、アーチ状エッジエアバッグが高圧気体を受けて内へ押圧するような変形を生じ、このように内筒体のキャビティのスペースを減少させ、適切な気圧に調整すると、開閉バルブを閉じて、導気管と給気装置の接続をオフにするステップ（１）と、
（2）、投入された基質の顆粒のサイズに応じて、遮断板とそれと隣接する弾性撹拌片との間の隙間を調整し、排気弁を開き、空気注入管を給気装置に外付けして、給気装置で空気注入管を介して横方向中空バーのキャビティに気体を注入し、高圧気体を横方向中空バーの通気孔、遮断板の吸気口および遮断エアバッグの導気分岐管を介して遮断エアバッグのキャビティに導入し、遮断エアバッグを高圧気体の作用下で拡張させて、遮断板とそれと隣接する弾性撹拌片との間の隙間のサイズを減少させ、適切な隙間に調整すると、排気弁を閉じて、空気注入管と外部との接続をオフにするステップ（２）と、
（3）、コントロールパネルを利用してサーボモーター、駆動モーターおよびトルクモーターを起動させ、サーボモーターの出力軸がカップリングを介してトルク研磨ロッドを回転駆動し、トルク研磨ロッドにおける雄ネジがボールナットを連動させて運動させ、それによってボールナットが駆動モーターを連動して運動させ、サーボモーターの出力軸が正逆回転を切り替えて駆動モーターを往復して上下動させ、駆動モーターの出力軸がカップリングを介してトルク回転軸を回転駆動し、駆動モーターが上下動すると同時に、トルク回転軸が補強軸受を介してスライダーブロックを連動してシュート内に上下動させ、トルク回転軸の運動の安定性を確保し、それと同時に、トルク回転軸が内筒体内における弾性撹拌片を回転駆動し、弾性撹拌片が回転すると同時に、内筒体内における基質と微生物菌株を混合して、弾性撹拌片はトルク回転軸から離れた一側でアーチ状エッジエアバッグの側辺に接触して押圧して、基質の内筒体内での回転スペースを減少させ、トルクモーターの出力軸が噛合歯車によって大歯車を介して内筒体を回転駆動して、内筒体内における基質が自体重力の作用下で反転するようにし、トルク回転軸の回転方向と内筒体の回転方向を逆にすることにより、内筒体の内側に位置する隙間遮断機構と弾性撹拌片を互いにずらして、基質を互いに押し付けて破砕し、基質を微生物菌株とよく混合して培養するステップ（３）を含む。
A method of producing a microbial strain, comprising:
(1) The substrate and the microbial strain to be produced are charged into the cavity of the inner cylinder from the charging port, and the mixing space of the substrate and the microbial strain in the cavity of the inner cylinder is adjusted according to the amount of the substrate charged. Open the open/close door, open the open/close valve, externally attach two air guide tubes to the air supply device, inject gas into the cavity of the arched edge airbag, and the arched edge airbag receives the high pressure gas. When the inner space of the cavity of the inner cylinder is reduced and the pressure is adjusted to an appropriate pressure, the opening/closing valve is closed and the connection between the air guide tube and the air supply device is turned off. (1),
(2) Adjust the gap between the shut-off plate and the adjacent elastic stirrer piece according to the size of the introduced substrate granules, open the exhaust valve, and attach the air injection pipe to the air supply device externally. The air supply device injects gas into the cavity of the horizontal hollow bar through the air injection pipe, and the high pressure gas is passed through the ventilation hole of the horizontal hollow bar, the intake port of the blocking plate and the air guide branch pipe of the blocking airbag. Through the cavity of the shut-off air bag and expand the shut-off air bag under the action of high-pressure gas to reduce the size of the gap between the shut-off plate and the adjacent elastic stirring piece, and adjust it to an appropriate gap. Then, closing the exhaust valve and turning off the connection between the air injection pipe and the outside (2),
(3) Using the control panel, the servo motor, drive motor and torque motor are started, the output shaft of the servo motor drives the torque polishing rod to rotate through the coupling, and the male screw on the torque polishing rod is the ball nut. The ball nut moves the drive motor in conjunction with it, and the output shaft of the servo motor switches forward and reverse rotation to reciprocate the drive motor to move up and down. The torque rotary shaft is driven to rotate through the ring, and the drive motor moves up and down. At the same time, the torque rotary shaft moves up and down in the chute in conjunction with the slider block via the reinforcing bearings, which stabilizes the motion of the torque rotary shaft. At the same time, the torque rotating shaft rotationally drives the elastic stirring piece in the inner cylinder, and at the same time the elastic stirring piece rotates, the substrate and the microbial strain in the inner cylinder are mixed, and the elastic stirring piece torque-rotates. One side away from the shaft touches and presses against the side of the arched edge airbag to reduce the rotational space within the inner cylinder of the substrate, and the output shaft of the torque motor through the gear wheel via the mesh gear. By rotating the inner cylinder so that the substrate in the inner cylinder reverses itself under the action of gravity, and by reversing the rotation direction of the torque rotation shaft and the rotation direction of the inner cylinder, The method includes a step (3) in which the gap blocking mechanism and the elastic stirring piece located inside are displaced from each other, the substrates are pressed against each other to be crushed, and the substrate is well mixed with the microbial strain and cultured.
従来技術に比べて、
（１）、該微生物菌株の製造装置および方法では、内筒体と内部押圧機構を設置して協力させることによって、装置の使用にあたって、基質顆粒の量に応じて内筒体内における基質が回転するスペースの大きさを調整することができる。開閉バルブを開いて、2本の導気管を給気装置に外付けしてアーチ状エッジエアバッグのキャビティに気体を注入し、アーチ状エッジエアバッグが高圧気体を受けて内へ押圧するような変形を生じ、それにより内筒体のキャビティのスペースを減少させる。装置が作動するとき、基質が重力の作用下で内筒体において反転して、大顆粒の顆粒基質が遮断板間の隙間により遮断されて小顆粒の顆粒基質と分離し、弾性撹拌片がトルク回転軸の回転による作用下で遮断板と交差して重なると、遮断板の間における遮断された大顆粒異物を弾性撹拌片で圧潰し、このように微生物菌株を破砕した基質とよく混合し、微生物菌株の基質での栄養素吸収率の向上に寄与するとともに、弾性撹拌片の回転による作用下で、弾性撹拌片の比較的薄い側辺が保護シートを介してアーチ状エッジエアバッグのアーチ状の内側辺に接触して押圧し、弾性撹拌片がアーチ状エッジエアバッグに接触した直後、弾性撹拌片による押圧により接触していないアーチ状エッジエアバッグの部分が押圧部分の気圧の作用により拡張されて、基質の内筒体内での反転スペースを減少させ、弾性撹拌片が回転して撹拌するに伴い、弾性撹拌片の外側辺による、内筒体の内側壁の回転方向における撹拌がある程度アーチ状に折り曲げられて、顆粒基質を段階的に押圧して、それによって基質を微生物菌株と効果的に混合するという目的を達成させる。
（２）、該微生物菌株の製造装置および方法では、弾性撹拌片と隙間遮断機構を設置して互いに協力させることによって、装置の使用にあたって、投入される基質の顆粒のサイズに応じて、遮断板同士が離れた隙間を調整して、排気弁を開き、空気注入管を給気装置に外付けして、給気装置で空気注入管を介して横方向中空バーのキャビティに気体を注入し、高圧気体を横方向中空バーの通気孔、遮断板の吸気口および遮断エアバッグの導気分岐管を介して遮断エアバッグのキャビティに導入し、遮断エアバッグが高圧気体の作用で拡張し、このように遮断板とそれと隣接する弾性撹拌片との間の隙間の大きさを減少させ、適切な隙間に調整されると、装置が作動するとき、弾性撹拌片は、遮断板の両側辺に気体を注入して拡張させた遮断エアバッグと接触し、それによって大顆粒基質が遮断板、弾性撹拌片および遮断エアバッグによる互いのソフト押圧の作用で再破砕され、このように、装置が微生物菌株を各種顆粒基質において混合する場合の均一混合率を高め、また、排気弁の使用により、弾性撹拌片が遮断エアバッグに押圧するように接触するとき、遮断エアバッグ内の気圧の単位面積当たりの圧力の安定性を確保し、互いに押圧するとき、単位面積当たりの圧力が高すぎることにより遮断エアバッグが破損されることが避けられ、排気弁により遮断エアバッグ内の単位面積当たりの圧力の安定性が確保され、さらに機構同士が安定して協力して使用されることが確保される。
（３）、該微生物菌株の製造装置および方法では、内筒体とサーボモーターを設置して協力させることによって、装置の使用にあたって、サーボモーターの出力軸が正逆回転して回転方向を切り替えることで、ボールナットが雄ネジの作用で駆動モーターを連動して上下動させ、駆動モーターの出力軸がカップリングを介してトルク回転軸を回転駆動し、駆動モーターが上下動すると同時に、カップリングを介してトルク回転軸を連動して上下動かし、それによってトルク回転軸が補強軸受を介してスライダーブロックを連動してシュート内で運動させ、トルク回転軸の回転安定性を確保し、また、トルク回転軸が上下動すると同時に、トルク回転軸のシールリングの内側壁に接触する側辺が対向運動し、弾性ゴムスリーブが圧縮または引張られ、それによって、内筒体に基質を投入して混合するときのシール性および各機構同士の協力の安定性を確保し、さらに破砕された顆粒基質と微生物菌株をよく混合する。
Compared with the conventional technology,
(1) In the apparatus and method for producing the microbial strain, the inner cylinder and the internal pressing mechanism are installed and cooperated with each other, whereby the substrate in the inner cylinder rotates according to the amount of the substrate granules when the apparatus is used. You can adjust the size of the space. Open the on-off valve, attach two air guide tubes to the air supply device, inject gas into the cavity of the arched edge airbag, and the arched edge airbag receives high pressure gas and presses it inward. Deformation occurs, which reduces the space of the cavity of the inner cylinder. When the device is activated, the matrix is inverted in the inner cylinder under the action of gravity, the granule matrix of the large granules is blocked by the gap between the barrier plates and separated from the granule matrix of the small granules, and the elastic stirrer strips torque. When it intersects and overlaps with the blocking plate under the action of the rotation of the rotating shaft, the blocked large granular foreign matter between the blocking plates is crushed with an elastic stirring piece, and thus mixed well with the crushed substrate of the microbial strain, the microbial strain In addition to contributing to the improvement of the nutrient absorption rate in the substrate, the relatively thin side of the elastic stirrer piece is inserted into the arched inner side of the airbag via the protective sheet under the action of the rotation of the elastic stirrer piece. Immediately after the elastic stirring piece comes into contact with and presses against the arched edge airbag, the portion of the arched edge airbag that is not in contact due to the pressing by the elastic stirring piece is expanded by the action of the atmospheric pressure of the pressing portion, The inversion space of the substrate in the inner cylinder is reduced, and as the elastic stirring piece rotates and stirs, the stirring in the rotation direction of the inner wall of the inner cylinder by the outer side of the elastic stirring piece is bent to an arc shape to some extent. The granular substrate is gradually pressed, thereby achieving the purpose of effectively mixing the substrate with the microbial strain.
(2) In the apparatus and method for producing a microbial strain, an elastic stirring piece and a gap blocking mechanism are installed to cooperate with each other, so that when the apparatus is used, a blocking plate is provided according to the size of the substrate granules to be charged. Adjust the gap apart from each other, open the exhaust valve, externally attach the air injection pipe to the air supply device, inject gas into the cavity of the horizontal hollow bar via the air injection pipe in the air supply device, The high-pressure gas is introduced into the cavity of the barrier airbag through the ventilation hole of the horizontal hollow bar, the intake port of the barrier plate and the air guide branch pipe of the barrier airbag, and the barrier airbag is expanded by the action of the high-pressure gas. As the size of the gap between the blocking plate and its adjacent elastic stirrer is reduced and adjusted to the appropriate gap, the elastic stirrer will not be able to reach the gas on both sides of the blocking plate when the device is operated. In contact with an expanded barrier air bag, whereby the large granular substrate is re-crushed by the action of the soft pressing of the barrier plate, elastic stirrer piece and barrier air bag against each other, thus the device is In order to increase the uniform mixing rate in the case of mixing in various granular substrates, and when the elastic stirring piece comes into contact with the blocking airbag so as to press it by using an exhaust valve, Ensures pressure stability and avoids damaging the shutoff airbag due to too high pressure per unit area when pressing against each other, and exhaust valve stabilizes pressure per unit area in the shutoff airbag It is ensured that the mechanism is used and that the mechanisms are used in cooperation with each other in a stable manner.
(3) In the apparatus and method for producing the microbial strain, the inner cylinder and the servo motor are installed to cooperate with each other, and the output shaft of the servo motor rotates forward and backward to switch the rotation direction when the apparatus is used. Then, the ball nut moves the drive motor up and down by the action of the male screw, and the output shaft of the drive motor rotationally drives the torque rotation shaft through the coupling. The torque rotary shaft is moved up and down in conjunction with each other, whereby the torque rotary shaft interlocks with the slider block through the reinforcing bearing to move in the chute, ensuring the rotational stability of the torque rotary shaft, and also the torque rotation. At the same time as the shaft moves up and down, the side contacting the inner wall of the seal ring of the torque rotating shaft moves oppositely, and the elastic rubber sleeve is compressed or pulled, whereby the substrate is put into the inner cylinder and mixed. It secures the sealing property of and the stability of cooperation between each mechanism, and further mixes the crushed granular substrate and the microbial strain well.
図１−６に示されるように、本発明は、以下の技術案を提供する。本体１、軸受ホルダー２、内筒体３、仕込み口４、大歯車５、トルクモーター６、噛合歯車７、サーボモーター８、トルク研磨ロッド９、雄ネジ１０、ボールナット１１、２つのロケートリング１２、駆動モーター１３、トルク回転軸１４、アダプタプレート１５、シュート１６、補強軸受１７、スライダーブロック１８、弾性撹拌片１９、２つの内部押圧機構２０、隙間遮断機構２１、コントロールパネル２２、弾性ゴムスリーブ２３およびシールリング２４を含む微生物菌株の製造装置であって、軸受ホルダー２はボルトにより本体１の内側の底部に固定して取り付けられ、本体１の正面に開閉ドアが設置され、内筒体３は本体１の内側に設置されかつ軸受ホルダー２の軸受内輪を貫通し、仕込み口４は内筒体３の左側に設置されかつ本体１の左側の内壁を貫通し、大歯車５は内筒体３に挿通され、トルクモーター６は固定台を介して本体１の内側に固定して取り付けられ、噛合歯車７は大歯車５の歯と噛み合ってかつトルクモーター６の出力軸に套設され、噛合歯車７は大歯車５を回転駆動し、内筒体３の回転数を効果的に低下させる。保障機構の間にサーボモーター８はボルトにより本体１の内側の最上部に固定して取り付けられ、サーボモーター８の出力軸がカップリングを介してトルク研磨ロッド９に接続され、トルク研磨ロッド９の末端が軸受ベースを介して本体１の内側の底部に移動可能に接続され、雄ネジ１０はトルク研磨ロッド９の円弧状側面に形成されており、ボールナット１１はトルク研磨ロッド９の雄ネジ１０部分に螺合され、２つのロケートリング１２はそれぞれトルク研磨ロッド９の雄ネジ１０部分の両端に挿通され、ロケートリング１２はボールナット１１を制限し、駆動モーター１３はボールナット１１の左側面に固定して取り付けられ、駆動モーター１３として、回転数１０−１５ｒ／ｍｉｎの低速駆動モーターが使用される。駆動モーター１３の出力軸がカップリングを介してトルク回転軸１４に接続され、アダプタプレート１５は本体１の内側の最上部と底部の間に固定して取り付けられ、シュート１６はアダプタプレート１５の側面に開けられ、スライダーブロック１８はシュート１６の内側に移動可能に接続され、補強軸受１７はスライダーブロック１８の内側に固定して取り付けられ、弾性ゴムスリーブ２３は内筒体３の右端面の貫通孔の内側に固定して取り付けられ、シールリング２４は弾性ゴムスリーブ２３の内側に固定して取り付けられ、トルク回転軸１４の駆動モーター１３から離れた一端が補強軸受１７とシールリング２４の内側を貫通して内筒体３の内側まで延伸している。補強軸受１７は内筒体３の真右側に位置し、トルク回転軸１４とシールリング２４は中間ばめの嵌合関係を有し、弾性ゴムスリーブ２３の初期状態が圧縮状態である。弾性撹拌片１９はトルク回転軸１４の内筒体３の内側に位置する部分に均等に取り付けられ、２つの内部押圧機構２０はいずれも内筒体３の内側に横方向に取り付けられ、隙間遮断機構２１は横方向に内筒体３の内側に取り付けられ、コントロールパネル２２は本体１の左側に設置される。コントロールパネル２２は、駆動モーター１３、トルクモーター６およびサーボモーター８に電気的に接続され、コントロールパネル２２は、駆動モーター１３、トルクモーター６およびサーボモーター８の起動・停止および作動状態を制御することができ、サーボモーター８として、回転数１０−１５ｒ／ｍｉｎの低速正逆回転モーターが使用される。 As shown in FIGS. 1-6, the present invention provides the following technical solutions. Main body 1, bearing holder 2, inner cylinder 3, charging port 4, large gear 5, torque motor 6, meshing gear 7, servo motor 8, torque polishing rod 9, male screw 10, ball nut 11, two locate rings 12 , Drive motor 13, torque rotary shaft 14, adapter plate 15, chute 16, reinforcing bearing 17, slider block 18, elastic stirring piece 19, two internal pressing mechanisms 20, gap blocking mechanism 21, control panel 22, elastic rubber sleeve 23. The bearing holder 2 is fixedly attached to the inner bottom of the main body 1 by a bolt, the opening/closing door is installed in front of the main body 1, and the inner cylindrical body 3 is It is installed inside the main body 1 and penetrates the bearing inner ring of the bearing holder 2, the charging port 4 is installed on the left side of the inner cylinder 3 and penetrates the inner wall on the left side of the main body 1, and the large gear 5 is the inner cylinder 3 , The torque motor 6 is fixedly attached to the inside of the main body 1 via a fixing base, and the meshing gear 7 meshes with the teeth of the large gear 5 and is installed on the output shaft of the torque motor 6, Reference numeral 7 rotationally drives the large gear 5 to effectively reduce the rotational speed of the inner cylindrical body 3. The servomotor 8 is fixedly attached to the innermost top portion of the main body 1 by a bolt during the guarantee mechanism, and the output shaft of the servomotor 8 is connected to the torque polishing rod 9 via a coupling, and The end is movably connected to the inner bottom of the body 1 via a bearing base, the male screw 10 is formed on the arcuate side surface of the torque polishing rod 9, and the ball nut 11 is the male screw 10 of the torque polishing rod 9. The two locate rings 12 are screwed into the respective parts, and the two locate rings 12 are respectively inserted into both ends of the male screw 10 part of the torque polishing rod 9, the locate ring 12 limits the ball nut 11, and the drive motor 13 is located on the left side surface of the ball nut 11. A low-speed drive motor having a rotational speed of 10-15 r/min is used as the drive motor 13 which is fixedly attached. The output shaft of the drive motor 13 is connected to the torque rotation shaft 14 via a coupling, the adapter plate 15 is fixedly attached between the top and bottom inside the main body 1, and the chute 16 is a side surface of the adapter plate 15. The slider block 18 is movably connected to the inside of the chute 16, the reinforcing bearing 17 is fixedly attached to the inside of the slider block 18, and the elastic rubber sleeve 23 is a through hole in the right end surface of the inner cylindrical body 3. The seal ring 24 is fixedly attached to the inside of the elastic rubber sleeve 23, and one end of the torque rotation shaft 14 away from the drive motor 13 penetrates the reinforcing bearing 17 and the inside of the seal ring 24. And extends to the inner side of the inner cylindrical body 3. The reinforcing bearing 17 is located on the right side of the inner cylindrical body 3, the torque rotary shaft 14 and the seal ring 24 have a fitting relationship of intermediate fitting, and the elastic rubber sleeve 23 is in an initial compressed state. The elastic stirring pieces 19 are evenly attached to the portion of the torque rotating shaft 14 located inside the inner tubular body 3, and the two internal pressing mechanisms 20 are both attached laterally inside the inner tubular body 3 to prevent gaps. The mechanism 21 is laterally mounted inside the inner cylindrical body 3, and the control panel 22 is installed on the left side of the main body 1. The control panel 22 is electrically connected to the drive motor 13, the torque motor 6, and the servo motor 8, and the control panel 22 controls start/stop and operating states of the drive motor 13, the torque motor 6, and the servo motor 8. As the servo motor 8, a low speed forward/reverse rotation motor having a rotation speed of 10-15 r/min is used.
弾性撹拌片１９のトルク回転軸１４から離れた側辺に保護シート１９１が粘着されている。弾性撹拌片１９は、材質として弾性プラスチックを使い、断面形状が円弧状であり、弾性撹拌片１９と内筒体３の内側壁との間に隙間を有し、弾性撹拌片１９はトルク回転軸１４から内筒体３の内側壁の側辺にかけて厚みが薄くなり、保護シート１９１は弾性撹拌片１９の内筒体３に近い側辺全体を粘着して包み、軟質ゴム材料を用いるものである。 A protective sheet 191 is adhered to the side of the elastic stirring piece 19 that is away from the torque rotation shaft 14. The elastic stirring piece 19 uses elastic plastic as a material, has an arc-shaped cross section, and has a gap between the elastic stirring piece 19 and the inner side wall of the inner cylindrical body 3, and the elastic stirring piece 19 is a torque rotating shaft. The thickness decreases from 14 to the side of the inner side wall of the inner cylindrical body 3, and the protective sheet 191 adheres and wraps the entire side of the elastic stirring piece 19 near the inner cylindrical body 3, and uses a soft rubber material. ..
２つの内部押圧機構２０は、２つのアーチ状エッジエアバッグ２０１、2本の導気管２０２および２つの開閉バルブ２０３を含み、２つのアーチ状エッジエアバッグ２０１がそれぞれ内筒体３の内側壁に粘着され、2本の導気管２０２がそれぞれ２つのアーチ状エッジエアバッグ２０１の導気口に設置され、2本の導気管２０２がそれぞれに内筒体３の対応内側壁を貫通して本体１のキャビティまで伸びており、２つの開閉バルブ２０３がそれぞれ2本の導気管２０２に取り付けられる。２つのアーチ状エッジエアバッグ２０１は、材質として軟質耐摩耗性ゴムを使い、アーチ状エッジエアバッグ２０１の側壁の厚みが６−８ミリメートルであり、アーチ状エッジエアバッグ２０１の内筒体３と接触する側辺と内筒体３は粘着により接続されている。 The two internal pressing mechanisms 20 include two arched edge airbags 201, two air guide tubes 202 and two opening/closing valves 203, and the two arched edge airbags 201 are respectively provided on the inner wall of the inner tubular body 3. The two air guide pipes 202 are attached to the air inlets of the two arch-shaped edge airbags 201, respectively, and the two air guide pipes 202 respectively penetrate the corresponding inner wall of the inner cylindrical body 3 and the main body 1 The two open/close valves 203 are attached to the two air guide tubes 202, respectively. The two arched edge airbags 201 use soft wear-resistant rubber as a material, the side walls of the arched edge airbag 201 have a thickness of 6-8 mm, and the inner tubular body 3 of the arched edge airbag 201 is The contacting side and the inner cylinder 3 are connected by adhesion.
隙間遮断機構２１は、横方向中空バー２１１、空気注入管２１２、排気弁２１３、接続ベース２１４、遮断板２１５、取付スロット２１６および遮断エアバッグ２１７を含み、内筒体３の内側壁に横方向バースロットが開けられ、横方向中空バー２１１が横方向バースロットを介して横方向に内筒体３の内側壁に取り付けられ、空気注入管２１２が横方向中空バー２１１の導気口に取り付けられ、空気注入管２１２が内筒体３の対応内側壁を貫通しかつ排気弁２１３に接続され、接続ベース２１４が横方向中空バー２１１の通気孔に固定して取り付けられ、接続ベース２１４の底部における導気孔が対応した通気孔に連通し、遮断板２１５が接続ベース２１４に固定して接続され、且つ遮断板２１５の接続ベース２１４に接続された側面が吸気口を通して接続ベース２１４の通気孔と互いに連通しており、取付スロット２１６が遮断板２１５の左右側面に対称的に開けられ、遮断エアバッグ２１７が取付スロット２１６の内側辺に粘着され、遮断エアバッグ２１７の導気分岐管が遮断板２１５の吸気口に固定して接続され、遮断エアバッグ２１７の外側辺が取付スロット２１６の内側から伸びて、対応した弾性撹拌片１９の側辺と接触する。遮断板２１５は、断面形状がアーチ状で、弾性プラスチック材質で製造され、遮断板２１５の内筒体３の内側壁に近い一側から内筒体３の内側の一側にかけて厚みが次第に薄くなる。遮断エアバッグ２１７は、軟質ゴム材料で製造され、遮断板２１５は接続ベース２１４を介して等距離で横方向中空バー２１１において横方向に配置されており、遮断板２１５と弾性撹拌片１９が交互に配置されており、遮断板２１５とそれと隣接する弾性撹拌片１９との間に隙間を有する。 The gap blocking mechanism 21 includes a horizontal hollow bar 211, an air injection pipe 212, an exhaust valve 213, a connection base 214, a blocking plate 215, a mounting slot 216, and a blocking airbag 217, and a horizontal direction on the inner wall of the inner tubular body 3. The bar slot is opened, the horizontal hollow bar 211 is laterally attached to the inner wall of the inner cylinder 3 through the horizontal bar slot, and the air injection pipe 212 is attached to the air inlet of the horizontal hollow bar 211. , The air injection pipe 212 penetrates the corresponding inner wall of the inner cylinder 3 and is connected to the exhaust valve 213, and the connection base 214 is fixedly attached to the ventilation hole of the horizontal hollow bar 211, and at the bottom of the connection base 214. The air guide hole communicates with the corresponding ventilation hole, the blocking plate 215 is fixedly connected to the connection base 214, and the side surface of the blocking plate 215 connected to the connection base 214 is connected to the ventilation hole of the connection base 214 through the intake port. In communication with each other, the mounting slots 216 are symmetrically opened on the left and right side surfaces of the blocking plate 215, the blocking airbag 217 is adhered to the inner side of the mounting slot 216, and the air guiding branch pipe of the blocking airbag 217 is blocked by the blocking plate 215. Is fixedly connected to the intake port of the blocking air bag 217, and the outer side of the shutoff airbag 217 extends from the inside of the mounting slot 216 to come into contact with the corresponding side of the elastic stirring piece 19. The blocking plate 215 has an arch-shaped cross section and is made of an elastic plastic material. The blocking plate 215 has a thickness that gradually decreases from one side of the blocking plate 215 near the inner wall of the inner cylindrical body 3 to one side of the inner cylindrical body 3. .. The blocking airbag 217 is made of a soft rubber material, and the blocking plate 215 is laterally arranged in the horizontal hollow bar 211 at an equal distance via the connection base 214, and the blocking plate 215 and the elastic stirring piece 19 are alternately arranged. And has a gap between the blocking plate 215 and the elastic stirring piece 19 adjacent thereto.
微生物菌株の製造方法であって、：
（1）、基質と製造すべき微生物菌株を仕込み口４から内筒体３のキャビティに投入して、基質の投入量に応じて内筒体３のキャビティにおける基質と微生物菌株の混合スペースを調整し、本体１の開閉ドアを開いて、開閉バルブ２０３を開き、2本の導気管２０２を給気装置に外付けしてアーチ状エッジエアバッグ２０１のキャビティに気体を注入し、アーチ状エッジエアバッグ２０１が高圧気体を受けて内へ押圧するような変形を生じ、このように内筒体３のキャビティのスペースを減少させ、適切な気圧に調整すると、開閉バルブ２０３を閉じて、導気管２０２と給気装置の接続をオフにするステップ（１）と、
（2）、投入された基質の顆粒のサイズに応じて、遮断板２１５とそれと隣接する弾性撹拌片１９との間の隙間を調整し、排気弁２１３を開き、空気注入管２１２を給気装置に外付けして、給気装置で空気注入管２１２を介して横方向中空バー２１１のキャビティに気体を注入し、高圧気体を横方向中空バー２１１の通気孔、遮断板２１５の吸気口および遮断エアバッグ２１７の導気分岐管を介して遮断エアバッグ２１７のキャビティに導入し、遮断エアバッグ２１７を高圧気体の作用下で拡張させて、遮断板２１５とそれと隣接する弾性撹拌片１９との間の隙間のサイズを減少させ、適切な隙間に調整すると、排気弁２１３を閉じて、空気注入管２１２と外部との接続をオフにするステップ（２）と、
（3）、コントロールパネル２２を利用してサーボモーター８、駆動モーター１３およびトルクモーター６を起動させ、サーボモーター８の出力軸がカップリングを介してトルク研磨ロッド９を回転駆動し、トルク研磨ロッド９における雄ネジ１０がボールナット１１を連動して運動させ、それによってボールナット１１が駆動モーター１３を連動して運動させ、サーボモーター８の出力軸が正逆回転を切り替えて駆動モーター１３を往復して上下動させ、駆動モーター１３の出力軸がカップリングを介してトルク回転軸１４を回転駆動し、駆動モーター１３が上下動すると同時に、トルク回転軸１４が補強軸受１７を介してスライダーブロック１８を連動してシュート１６内に上下動させ、トルク回転軸１４の運動の安定性を確保し、それと同時に、トルク回転軸１４が内筒体３内における弾性撹拌片１９を回転駆動し、弾性撹拌片１９が回転すると同時に、内筒体３内における基質と微生物菌株を混合して、弾性撹拌片１９はトルク回転軸１４から離れた一側でアーチ状エッジエアバッグ２０１の側辺に接触して押圧して、基質の内筒体３内での回転スペースを減少させ、トルクモーター６の出力軸が噛合歯車７によって大歯車５を介して内筒体３を回転駆動して、内筒体３内における基質が自体重力の作用下で反転するようにし、トルク回転軸１４の回転方向と内筒体３の回転方向を逆にすることにより、内筒体３の内側に位置する隙間遮断機構２１と弾性撹拌片１９を互いにずらして、基質を互いに押し付けて破砕し、基質を微生物菌株とよく混合して培養するステップ３とを含む。
A method of producing a microbial strain, comprising:
(1) The substrate and the microbial strain to be produced are charged into the cavity of the inner cylinder 3 from the charging port 4, and the mixing space of the substrate and the microbial strain in the cavity of the inner cylinder 3 is adjusted according to the amount of the substrate charged. Then, open the opening/closing door of the main body 1, open the opening/closing valve 203, externally attach the two air guide pipes 202 to the air supply device, inject gas into the cavity of the arched edge airbag 201, When the bag 201 receives a high-pressure gas and is deformed so as to be pressed inward, and thus the space of the cavity of the inner cylindrical body 3 is reduced and the pressure is adjusted to an appropriate atmospheric pressure, the on-off valve 203 is closed and the air guide pipe 202. (1) to turn off the connection between the air supply device and
(2) Adjusting the gap between the blocking plate 215 and the elastic stirring piece 19 adjacent thereto according to the size of the introduced granules of the substrate, opening the exhaust valve 213, and connecting the air injection pipe 212 to the air supply device. Externally, the gas is injected into the cavity of the horizontal hollow bar 211 via the air injection pipe 212 by the air supply device, and the high pressure gas is vented to the horizontal hollow bar 211, the intake port of the blocking plate 215, and cut off. The air is introduced into the cavity of the blocking airbag 217 via the air guiding branch pipe of the airbag 217, and the blocking airbag 217 is expanded under the action of the high-pressure gas so that the blocking plate 215 and the elastic stirring piece 19 adjacent thereto are expanded. When the size of the gap is reduced and adjusted to an appropriate gap, the step (2) of closing the exhaust valve 213 and turning off the connection between the air injection pipe 212 and the outside,
(3) Using the control panel 22, the servo motor 8, the drive motor 13, and the torque motor 6 are activated, and the output shaft of the servo motor 8 rotationally drives the torque polishing rod 9 via the coupling to generate the torque polishing rod. The male screw 10 in 9 causes the ball nut 11 to move in an interlocking manner, whereby the ball nut 11 causes the drive motor 13 to move in an interlocking manner, and the output shaft of the servo motor 8 switches forward and reverse rotations to reciprocate the drive motor 13. Then, the output shaft of the drive motor 13 rotationally drives the torque rotation shaft 14 via the coupling, and the drive motor 13 moves up and down, and at the same time, the torque rotation shaft 14 moves through the reinforcing bearing 17 and the slider block 18 Is moved up and down in the chute 16 in an interlocking manner to ensure the stability of the motion of the torque rotating shaft 14, and at the same time, the torque rotating shaft 14 rotationally drives the elastic stirring piece 19 in the inner cylindrical body 3 to perform elastic stirring. Simultaneously with the rotation of the piece 19, the substrate and the microbial strain in the inner cylinder 3 are mixed, and the elastic stirring piece 19 comes into contact with the side of the arched edge airbag 201 on one side away from the torque rotation shaft 14. By pressing, the rotation space of the substrate in the inner cylindrical body 3 is reduced, and the output shaft of the torque motor 6 rotationally drives the inner cylindrical body 3 via the large gear 5 by the meshing gear 7 to rotate the inner cylindrical body 3 The substrate in the inside is inverted under the action of gravity itself, and the rotation direction of the torque rotating shaft 14 and the rotation direction of the inner cylindrical body 3 are reversed, so that the gap blocking mechanism 21 located inside the inner cylindrical body 3 And step 3 in which the elastic stirring pieces 19 are displaced from each other, the substrates are pressed against each other to be crushed, and the substrate is well mixed with the microbial strain and cultured.
図中では、１本体、２軸受ホルダー、３内筒体、４仕込み口、５大歯車、６トルクモーター、７噛合歯車、８サーボモーター、９トルク研磨ロッド、１０雄ネジ、１１ボールナット、１２ロケートリング、１３駆動モーター、１４トルク回転軸、１５アダプタプレート、１６シュート、１７補強軸受、１８スライダーブロック、１９弾性撹拌片、１９１保護シート、２０内部押圧機構、２０１アーチ状エッジエアバッグ、２０２導気管、２０３開閉バルブ、２１隙間遮断機構、２１１横方向中空バー、２１２空気注入管、２１３排気弁、２１４接続ベース、２１５遮断板、２１６取付スロット、２１７遮断エアバッグ、２２コントロールパネル、２３弾性ゴムスリーブ、２４シールリング。
In the figure, 1 main body, 2 bearing holder, 3 inner cylinder, 4 charging port, 5 large gear, 6 torque motor, 7 meshing gear, 8 servo motor, 9 torque polishing rod, 10 male screw, 11 ball nut, 12 Locate ring, 13 drive motor, 14 torque rotary shaft, 15 adapter plate, 16 chute, 17 reinforcing bearing, 18 slider block, 19 elastic stirring piece, 191 protective sheet, 20 internal pressing mechanism, 201 arched edge airbag, 202 guide Trachea, 203 opening/closing valve, 21 clearance blocking mechanism, 211 lateral hollow bar, 212 air injection tube, 213 exhaust valve, 214 connection base, 215 blocking plate, 216 mounting slot, 217 blocking airbag, 22 control panel, 23 elastic rubber Sleeve, 24 seal ring.
Claims (8)
前記軸受ホルダー（２）はボルトにより本体（１）の内側の底部に固定して取り付けられ、本体（１）の正面に開閉ドアが設置され、内筒体（３）は本体（１）の内側に設置されかつ軸受ホルダー（２）の軸受内輪を貫通し、仕込み口（４）は内筒体（３）の左側に設置されかつ本体（１）の左側の内壁を貫通し、大歯車（５）は内筒体（３）に挿通され、トルクモーター（６）は固定台を介して本体（１）の内側に固定して取り付けられ、噛合歯車（７）は大歯車（５）の歯と噛み合ってかつトルクモーター（６）の出力軸に套設され、サーボモーター（８）はボルトにより本体（１）の内側の最上部に固定して取り付けられ、サーボモーター（８）の出力軸がカップリングを介してトルク研磨ロッド（９）に接続され、トルク研磨ロッド（９）の末端が軸受ベースを介して本体（１）の内側の底部に移動可能に接続され、雄ネジ（１０）はトルク研磨ロッド（９）の円弧状側面に形成されており、ボールナット（１１）はトルク研磨ロッド（９）の雄ネジ（１０）部分に螺合され、２つのロケートリング（１２）はそれぞれトルク研磨ロッド（９）の雄ネジ（１０）部分の両端に挿通され、駆動モーター（１３）はボールナット（１１）の左側面に固定して取り付けられ、駆動モーター（１３）の出力軸がカップリングを介してトルク回転軸（１４）に接続され、アダプタプレート（１５）は本体（１）の内側の最上部と底部の間に固定して取り付けられ、シュート（１６）はアダプタプレート（１５）の側面に開けられ、スライダーブロック（１８）はシュート（１６）の内側に移動可能に接続され、補強軸受（１７）はスライダーブロック（１８）の内側に固定して取り付けられ、弾性ゴムスリーブ（２３）は内筒体（３）の右端面の貫通孔の内側に固定して取り付けられ、シールリング（２４）は弾性ゴムスリーブ（２３）の内側に固定して取り付けられ、トルク回転軸（１４）の駆動モーター（１３）から離れた一端が補強軸受（１７）とシールリング（２４）の内側を貫通して内筒体（３）の内側まで延伸しており、弾性撹拌片（１９）はトルク回転軸（１４）の内筒体（３）の内側に位置する部分に均等に取り付けられ、２つの内部押圧機構（２０）はいずれも横方向に内筒体（３）の内側に取り付けられ、隙間遮断機構（２１）は横方向に内筒体（３）の内側に取り付けられ、コントロールパネル（２２）は本体（１）の左側に設置され、
前記弾性撹拌片（１９）のトルク回転軸（１４）から離れた側辺に保護シート（１９１）が粘着されており、
２つの前記内部押圧機構（２０）は、２つのアーチ状エッジエアバッグ（２０１）、2本の導気管（２０２）および２つの開閉バルブ（２０３）を含み、２つのアーチ状エッジエアバッグ（２０１）がそれぞれ内筒体（３）の内側壁に粘着され、2本の導気管（２０２）がそれぞれ２つのアーチ状エッジエアバッグ（２０１）の導気口に設置され、2本の導気管（２０２）がそれぞれ内筒体（３）の対応内側壁を貫通して本体（１）のキャビティまで伸びており、２つの開閉バルブ（２０３）がそれぞれ2本の導気管（２０２）に取り付けられ、
前記隙間遮断機構（２１）は、横方向中空バー（２１１）、空気注入管（２１２）、排気弁（２１３）、接続ベース（２１４）、遮断板（２１５）、取付スロット（２１６）および遮断エアバッグ（２１７）を含み、内筒体（３）の内側壁に横方向バースロットが開けられ、横方向中空バー（２１１）が横方向バースロットを介して横方向に内筒体（３）の内側壁に取り付けられ、空気注入管（２１２）が横方向中空バー（２１１）の導気口に取り付けられ、空気注入管（２１２）が内筒体（３）の対応内側壁を貫通しかつ排気弁（２１３）に接続され、接続ベース（２１４）が横方向中空バー（２１１）の通気孔に固定して取り付けられ、接続ベース（２１４）の底部における導気孔が対応した通気孔に連通し、遮断板（２１５）が接続ベース（２１４）に固定して接続され、且つ遮断板（２１５）の接続ベース（２１４）に接続された側面が吸気口を通して接続ベース（２１４）の通気孔と互いに連通しており、取付スロット（２１６）が遮断板（２１５）の左右側面に対称的に開けられ、遮断エアバッグ（２１７）が取付スロット（２１６）の内側辺に粘着され、遮断エアバッグ（２１７）の導気分岐管が遮断板（２１５）の吸気口に固定して接続され、遮断エアバッグ（２１７）の外側辺が取付スロット（２１６）の内側から伸びて、対応した弾性撹拌片（１９）の側辺と接触することを特徴とする微生物菌株の製造装置。 Main body (1), bearing holder (2), inner cylinder (3), charging port (4), large gear (5), torque motor (6), meshing gear (7), servomotor (8), torque polishing Rod (9), male screw (10), ball nut (11), two locate rings (12), drive motor (13), torque rotating shaft (14), adapter plate (15), chute (16), reinforcement Bearing (17), slider block (18), elastic stirring piece (19), two internal pressing mechanisms (20), gap blocking mechanism (21), control panel (22), elastic rubber sleeve (23) and seal ring ( 24) A microbial strain producing apparatus including:
The bearing holder (2) is fixedly attached to the inner bottom portion of the main body (1) with a bolt, an opening/closing door is installed in front of the main body (1), and the inner tubular body (3) is inside the main body (1). Installed through the bearing inner ring of the bearing holder (2), the charging port (4) is installed on the left side of the inner cylindrical body (3) and penetrates the inner wall on the left side of the main body (1), ) Is inserted into the inner cylindrical body (3), the torque motor (6) is fixedly attached to the inside of the main body (1) via a fixing base, and the meshing gear (7) is attached to the teeth of the large gear (5). The output shaft of the servomotor (8) is meshed with and mounted on the output shaft of the torque motor (6), and the servomotor (8) is fixedly attached to the uppermost part inside the main body (1) by a bolt, and the output shaft of the servomotor (8) is a cup. It is connected to a torque polishing rod (9) via a ring, the end of the torque polishing rod (9) is movably connected to the inner bottom of the body (1) via a bearing base, and the male screw (10) is torqued. The ball nut (11) is formed on the arcuate side surface of the polishing rod (9), and the ball nut (11) is screwed onto the male screw (10) of the torque polishing rod (9), and the two locate rings (12) are respectively torque-polished. The drive motor (13) is fixedly attached to the left side surface of the ball nut (11) by being inserted into both ends of the male screw (10) part of the rod (9), and the output shaft of the drive motor (13) is coupled. Connected to the torque rotation shaft (14) via the adapter plate (15) fixedly mounted between the top and bottom inside the body (1) and the chute (16) on the side of the adapter plate (15). The slider block (18) is movably connected to the inside of the chute (16), the reinforcing bearing (17) is fixedly attached to the inside of the slider block (18), and the elastic rubber sleeve (23) is The inner ring (3) is fixedly mounted inside the through hole of the right end surface, and the seal ring (24) is fixedly mounted inside the elastic rubber sleeve (23) to drive the torque rotating shaft (14). One end away from the motor (13) penetrates the inside of the reinforcing bearing (17) and the seal ring (24) and extends to the inside of the inner cylindrical body (3), and the elastic stirring piece (19) is a torque rotating shaft. (14) is evenly attached to a portion located inside the inner cylindrical body (3), and the two internal pressing mechanisms (20) are both laterally attached to the inner side of the inner cylindrical body (3) to block the gap. The mechanism (21) is laterally mounted inside the inner cylindrical body (3) and has a control panel (22). ) Is installed on the left side of the body (1),
A protective sheet (191) is adhered to the side of the elastic stirring piece (19) remote from the torque rotation shaft (14),
The two internal pressing mechanisms (20) include two arched edge airbags (201), two air guide tubes (202) and two open/close valves (203), and two arched edge airbags (201). ) Is adhered to the inner wall of the inner cylindrical body (3), and the two air guide tubes (202) are installed at the air inlets of the two arched edge airbags (201), respectively. 202) extend through the corresponding inner side wall of the inner cylindrical body (3) to the cavity of the main body (1), and two open/close valves (203) are attached to the two air guide tubes (202),
The gap blocking mechanism (21) includes a horizontal hollow bar (211), an air injection pipe (212), an exhaust valve (213), a connection base (214), a blocking plate (215), a mounting slot (216) and a blocking air. A lateral bar slot is opened in the inner wall of the inner cylinder (3), including the bag (217), and a horizontal hollow bar (211) is provided laterally of the inner cylinder (3) through the lateral bar slot. Attached to the inner wall, the air injection pipe (212) is attached to the air inlet of the lateral hollow bar (211), the air injection pipe (212) penetrates the corresponding inner wall of the inner cylinder (3) and exhausts. Connected to the valve (213), the connection base (214) is fixedly attached to the ventilation hole of the lateral hollow bar (211), the air guide hole at the bottom of the connection base (214) communicates with the corresponding ventilation hole, The blocking plate (215) is fixedly connected to the connection base (214), and the side surface of the blocking plate (215) connected to the connection base (214) communicates with the ventilation hole of the connection base (214) through the intake port. The mounting slots (216) are symmetrically opened on the left and right sides of the blocking plate (215), and the blocking airbag (217) is adhered to the inner side of the mounting slot (216) to block the blocking airbag (217). Is connected to the intake port of the blocking plate (215) in a fixed manner, the outer side of the blocking airbag (217) extends from the inside of the mounting slot (216), and the corresponding elastic stirring piece (19). An apparatus for producing a microbial strain, which is in contact with the side of the.
基質と製造すべき微生物菌株を仕込み口（４）から内筒体（３）のキャビティに投入して、基質の投入量に応じて内筒体（３）のキャビティにおける基質と微生物菌株の混合スペースを調整し、本体（１）の開閉ドアを開いて、開閉バルブ（２０３）を開き、2本の導気管（２０２）を給気装置に外付けしてアーチ状エッジエアバッグ（２０１）のキャビティに気体を注入し、アーチ状エッジエアバッグ（２０１）が高圧気体を受けて内へ押圧するような変形を生じ、このように内筒体（３）のキャビティのスペースを減少させ、適切な気圧に調整すると、開閉バルブ（２０３）を閉じて、導気管（２０２）と給気装置の接続をオフにするステップ（１）と、
投入された基質の顆粒のサイズに応じて、遮断板（２１５）とそれと隣接する弾性撹拌片（１９）との間の隙間を調整し、排気弁（２１３）を開き、空気注入管（２１２）を給気装置に外付けして、給気装置で空気注入管（２１２）を介して横方向中空バー（２１１）のキャビティに気体を注入し、高圧気体を横方向中空バー（２１１）の通気孔、遮断板（２１５）の吸気口および遮断エアバッグ（２１７）の導気分岐管を介して遮断エアバッグ（２１７）のキャビティに導入し、遮断エアバッグ（２１７）を高圧気体の作用下で拡張させて、遮断板（２１５）とそれと隣接する弾性撹拌片（１９）との間の隙間のサイズを減少させ、適切な隙間に調整すると、排気弁（２１３）を閉じて、空気注入管（２１２）と外部との接続をオフにするステップ（２）と、
コントロールパネル（２２）を利用してサーボモーター（８）、駆動モーター（１３）およびトルクモーター（６）を起動させ、サーボモーター（８）の出力軸がカップリングを介してトルク研磨ロッド（９）を回転駆動し、トルク研磨ロッド（９）における雄ネジ（１０）がボールナット（１１）を連動して運動させ、それによってボールナット（１１）が駆動モーター（１３）を連動して運動させ、サーボモーター（８）の出力軸が正逆回転を切り替えて駆動モーター（１３）を往復して上下動させ、駆動モーター（１３）の出力軸がカップリングを介してトルク回転軸（１４）を回転駆動し、駆動モーター（１３）が上下動すると同時に、トルク回転軸（１４）が補強軸受（１７）を介してスライダーブロック（１８）を連動してシュート（１６）内に上下動させ、トルク回転軸（１４）の運動の安定性を確保し、それと同時に、トルク回転軸（１４）が内筒体（３）内における弾性撹拌片（１９）を回転駆動し、弾性撹拌片（１９）が回転すると同時に、内筒体（３）内における基質と微生物菌株を混合して、弾性撹拌片（１９）はトルク回転軸（１４）から離れた一側でアーチ状エッジエアバッグ（２０１）の側辺に接触して押圧して、基質の内筒体（３）内での回転スペースを減少させ、トルクモーター（６）の出力軸が噛合歯車（７）によって大歯車（５）を介して内筒体（３）を回転駆動して、内筒体（３）内における基質が自体重力の作用下で反転するようにし、トルク回転軸（１４）の回転方向と内筒体（３）の回転方向を逆にすることにより、内筒体（３）の内側に位置する隙間遮断機構（２１）と弾性撹拌片（１９）を互いにずらして、基質を互いに押し付けて破砕し、基質を微生物菌株とよく混合して培養するステップ（３）とを含む、ことを特徴とする製造方法。
A method of producing a microbial strain, comprising:
The substrate and the microbial strain to be produced are charged into the cavity of the inner cylinder (3) from the charging port (4), and the mixed space of the substrate and the microbial strain in the cavity of the inner cylinder (3) is charged according to the amount of the substrate charged. And open the open/close door of the main body (1), open the open/close valve (203), and attach the two air guide pipes (202) to the air supply device externally to form the cavity of the arched edge airbag (201). Gas is injected into the arched edge airbag (201) to receive high-pressure gas and deform so as to press it inward, thus reducing the space of the cavity of the inner tubular body (3) and reducing the pressure to an appropriate level. When the adjustment is made to step (1), the on-off valve (203) is closed, and the connection between the air guide tube (202) and the air supply device is turned off.
The clearance between the blocking plate (215) and the elastic stirring piece (19) adjacent to the blocking plate (215) is adjusted according to the size of the introduced substrate granules, the exhaust valve (213) is opened, and the air injection pipe (212) is opened. Is externally attached to the air supply device, and gas is injected into the cavity of the horizontal hollow bar (211) through the air injection pipe (212) in the air supply device, and high-pressure gas is passed through the horizontal hollow bar (211). The air is introduced into the cavity of the shutoff air bag (217) through the pores, the intake port of the shutoff plate (215) and the air guiding branch pipe of the shutoff air bag (217), and the shutoff air bag (217) is operated under the action of high pressure gas. When it is expanded to reduce the size of the gap between the blocking plate (215) and the elastic stirring piece (19) adjacent to the blocking plate (215) and adjusted to an appropriate gap, the exhaust valve (213) is closed and the air injection pipe ( 212) and the step (2) of turning off the connection with the outside,
The servomotor (8), the drive motor (13) and the torque motor (6) are activated using the control panel (22), and the output shaft of the servomotor (8) is coupled to the torque polishing rod (9). The male screw (10) of the torque polishing rod (9) moves the ball nut (11) in an interlocking manner, whereby the ball nut (11) moves the drive motor (13) in an interlocking manner. The output shaft of the servo motor (8) switches between forward and reverse rotation to reciprocate the drive motor (13) to move up and down, and the output shaft of the drive motor (13) rotates the torque rotation shaft (14) via a coupling. At the same time as the drive motor (13) moves up and down, the torque rotation shaft (14) moves the slider block (18) up and down in the chute (16) through the reinforcing bearing (17) to rotate the torque. The stability of the movement of the shaft (14) is ensured, and at the same time, the torque rotation shaft (14) rotationally drives the elastic stirring piece (19) in the inner cylindrical body (3), and the elastic stirring piece (19) rotates. At the same time, the substrate in the inner cylinder (3) is mixed with the microbial strain, and the elastic stirring piece (19) is provided on one side away from the torque rotation shaft (14) to the side of the arched edge airbag (201). To press and reduce the rotation space of the substrate in the inner cylinder body (3), and the output shaft of the torque motor (6) is engaged by the meshing gear (7) through the large gear (5) to the inner cylinder. The body (3) is rotationally driven so that the substrate in the inner cylindrical body (3) is inverted under the action of gravity itself, and the rotation direction of the torque rotation shaft (14) and the rotation direction of the inner cylindrical body (3). By reversing, the gap blocking mechanism (21) and the elastic stirring piece (19) located inside the inner tubular body (3) are displaced from each other, and the substrates are pressed against each other to be crushed, and the substrate is often mixed with the microbial strain. And (3) mixing and culturing.
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| CN201811485448.1 | 2018-12-06 | ||
| CN201811485448.1A CN109554276A (en) | 2018-12-06 | 2018-12-06 | A kind of Preparation equipment and method of microbial strains |
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| CN112831398A (en) * | 2021-01-22 | 2021-05-25 | 湖北凌卓生物工程有限公司 | Quick activation propagation device of microbial feed additive |
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- 2018-12-06 CN CN201811485448.1A patent/CN109554276A/en not_active Withdrawn
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Also Published As
| Publication number | Publication date |
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| CN109554276A (en) | 2019-04-02 |
| JP6812498B2 (en) | 2021-01-13 |
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