JP2020037533A - Skin external preparation - Google Patents

Abstract

To provide a skin external preparation that uses dead cells of lactic acid bacteria, oligosaccharides and bittern in combination, thereby improving moisture retention of skin.SOLUTION: A skin external preparation contains the following (A)-(C): (A) dead cells of lactic acid bacteria, (B) oligosaccharides, and (C) bittern.SELECTED DRAWING: Figure 1

Description

  The present invention relates to an external preparation for skin comprising a combination of killed cells of lactic acid bacteria, oligosaccharides and bittern.

It is known that the cells of Lactococci Enterococcus faecalis EC-12, especially the heat-killed cells, have the effect of promoting the production of glycerol derived from Staphylococcus epidermidis and the production of antimicrobial peptides derived from skin epidermal keratinocytes. Have been. (Patent Document 1)
It is known that gluco-oligosaccharide has an action as a control agent for bacteria resident on the skin and an action to promote attachment of Staphylococcus epidermidis to the skin surface. (Patent Document 2)
It is known that an ecosystem balance regulator of skin-resident bacteria containing bittern as an active ingredient does not have a bactericidal effect on beneficial bacteria, Staphylococcus epidermidis. (Patent Document 3)

JP, 2017-101006, A JP 2005-2087 A JP 2005-139075 A

  The killed cells of lactic acid bacteria were insoluble and it was difficult to mix them in a large amount in a skin external preparation. The use of a large amount of oligosaccharides affected the feeling of use such as stickiness. It is also reported that bittern requires a large amount of the compound to exert its effect. An object of the present invention is to provide an external preparation for skin that improves the moisturizing property of the skin by using a killed lactic acid bacterium, an oligosaccharide, and a bittern.

The present invention
A skin external preparation (A) containing killed cells of lactic acid bacteria (B) oligosaccharide (C) bittern containing the following (A) to (C):

  By using the killed lactic acid bacteria, oligosaccharides and bittern in the skin external preparation of the present invention in combination, Staphylococcus epidermidis, a useful resident bacterium, multiplies, and the moisturizing power of the skin is synergistically improved.

FIG. 1 is a diagram showing that the growth ratio of Staphylococcus epidermidis is synergistically improved by using three components in combination. FIG. 2: is a figure which shows that a skin horny layer water content improves synergistically by using 3 components together. FIG. 3 is a diagram showing that the transepidermal water loss is synergistically reduced by using three components in combination.

  Hereinafter, embodiments for carrying out the present invention will be described.

  The external preparation for skin of the present invention can be used for any of cosmetics, quasi-drugs, pharmaceuticals and the like.

  The present invention is an external preparation for skin containing dead cells of lactic acid bacteria as an essential component.

The lactic acid bacteria include Streptococcus, Lactococcus, Enterococcus, Lactobacillus, Pediococcus, Leuconostoc, and Weissella. It is preferably at least one strain selected from the group consisting of the genus and the genus Bifidobacterium. Particularly preferred is Enterococcus faecalis.

  The method for culturing lactic acid bacterium is not particularly limited, including a conventionally known method for culturing lactic acid bacterium. Heat-killed cells can be produced by the following method. From the culture obtained by culturing the lactic acid bacterium according to a conventional method, for example, the cells are collected by filtration, centrifugation, etc., washed with water, suspended in water, etc., and heat-treated. It can be adjusted by concentrating and drying. It may be carried out using a commonly used spray dryer, freeze dryer or the like. In some cases, before or after sterilization by heating or before or after drying, enzyme treatment, surfactant treatment, grinding and pulverization can also be performed, and those obtained by these treatments can be used in the present invention. Of dead cells.

  The water used for washing and suspension includes pure water, tap water, well water, mineral spring water, mineral water, hot spring water, spring water, fresh water, and the like, as well as those subjected to various treatments. Examples of the treatment applied to water include purification, heating, sterilization, filtration, ion exchange, adjustment of osmotic pressure, buffering, and the like. Therefore, the water that can be used as an extraction solvent in the present invention includes purified water, heat-treated water, ion-exchanged water, physiological saline, various buffers, and the like.

  In the present invention, the amount of the killed lactic acid bacteria in the preparation is usually 0.01 to 2% by mass, particularly preferably 0.01 to 1% by mass.

  The external preparation for skin of the present invention contains an oligosaccharide. By blending the oligosaccharide, the moisturizing effect can be improved.

  Examples of the oligosaccharide used in the present invention include xylo-oligosaccharides such as xylobiose, xylotriose, xylotetraose, and xylopentaose, galacto-oligosaccharides such as agarobiose and carabiose, glucotetraose, maltose, maltotriose, maltotetraose, and maltose. Glucooligosaccharides such as pentaose, isomaltose, sophorose, cellobiose, cellotriose, cellotetraose, cellopentaose, trehalose, neotrehalose, and isotrehalose; mannooligosaccharides; inulobiose; inulotriose; inulotetraose; inulotetraose; Homo-oligosaccharides such as fructooligosaccharides, bicyanose, isoprimeverose, sambubiose, primeverose, lycotetraose, sorabiose, Biose, manninotriose, lactose, lycobiose, lycotriose, epicellobiose, sucrose, turanose, maltulose, isokestose, erulose, kestose, gentianose, lactulose, epigentibiose, isorikunose, umbelliferose, sesamorse, raffinose Hetero-oligosaccharides such as robinobiose, silanobiose, rutinose, cacotriose, solatriose, and glycosyltrehalose.

  Glucoligosaccharide is preferably used as the oligosaccharide. Among them, glucotetraose is preferred. Glucotetraose is obtained from the natural sugars maltose and sucrose by an immobilized enzyme method. For example, (O-α-D-glucopyranosyl- (1 → 2))-(O-α-D-glucopyranosyl- (1 → 6))-(O-α-D-glucopyranosyl- (1 → 4))- (O-α-D-glucopyranose). Such a gluco-oligosaccharide is commercially available, for example, as BIOECOLIA (registered trademark: Solabia).

  The amount of the oligosaccharide to be added to the preparation in the present invention is usually 0.01 to 20% by mass, particularly preferably 0.01 to 10% by mass.

  As the bittern used in the present invention, in producing salt from seawater, a liquid obtained by crystallizing salt or a dried product thereof, or a product obtained by artificially mixing a mineral component is used. The composition of bittern varies depending on the locality of production, differences in salt production methods (ion exchange membrane method, solar method, salt field method or evaporation method), temperature during salt crystallization, salt concentration and pressure during salt crystallization and other conditions. However, bittern prepared using seawater collected from the sea area around the Tokara Islands or the sea area around Minami Daito Island has no contamination of contaminants such as organic phosphate compounds contained in the seawater, and a high effect can be obtained.

  The amount of bittern to be added to the preparation in the present invention is usually 0.0001 to 5% by mass, particularly preferably 0.01 to 3% by mass.

  In addition to the above-mentioned essential components, the skin external preparation of the present invention usually contains an aqueous component, an oily component, a humectant, a pigment, a surfactant, an ultraviolet absorber, and a thickener, which are usually added to the skin external preparation as needed. Agents, beauty ingredients, fragrances, polymeric substances, antibacterial and antimicrobial agents, alcohols, powders, scrubbing agents, biological components and the like can be appropriately compounded.

  The external preparation for skin of the present invention can be used, for example, in the form of lotions, emulsions, and ointments. Further, the method for producing the external preparation for skin of the present invention does not matter.

  The effect of the external preparation for skin of the present invention was measured as follows.

Examples and comparative examples having the compositions shown in Table 1 were prepared, and their effects on Staphylococcus epidermidis were examined.
[Test substance]
Lactobacilli killed cells: La Flora EC-12 (manufacturer: Ichimaru Falcos Co., Ltd.) was used.
Oligosaccharide: BIOECOLIA (manufacturer: Solabia) was used.
Nigari: Tokara Nigari N (manufacturer: Noevir) was used.
[Test strain]
Staphylococcus epidermidis (NBRC No. 12993) was adopted as a beneficial bacterium.

[Evaluation of Staphylococcus epidermidis multiplication effect]
Into a 96-well microplate, 100 μL of a test substance solution diluted to a predetermined concentration in a normal bouillon medium (Eiken Chemical) is added. Subsequently, 100 μL of Staphylococcus epidermidis adjusted to OD ₆₅₀ = 0.05 was added dropwise. After shaking culture at 37 ° C. for 6 hours, measurement was performed at OD 650 nm, and the viable cell count of each bacterium was measured. The growth effect on bacteria was evaluated by a relative value when Comparative Example 4 was set to 100. Table 1 shows the results.

[Usage test]
Examples and comparative examples having the compositions shown in Table 1 were prepared, and use tests were performed.
Three males aged 27 to 44 (mean age 38 years) were used as test subjects, and 9 μL of a skin external preparation was dropped and used twice daily in the morning and evening on a 3 cm × 3 cm area of the forearm. Before and one week after use, the water content of the epidermal skin layer and the transepidermal water loss were measured.

[Measuring method]
(1) Acclimation After cleaning the forearm, the subject was acclimated for 15 minutes in a room conditioned at a temperature of 21 ° C. and a humidity of 50% to prepare initial conditions and measure.

(2) Skin superficial stratum corneum water content Using SKICON-200EX, 5 points of the forearm were measured, and the average value was defined as the skin superficial stratum corneum water content. Relative values were calculated assuming that the water content of the skin surface horny layer before the use test was 1, and the results are shown in Table 1.

(3) Transepidermal Water Evaporation Two points on the forearm were measured using a Vapometer, and the average value was taken as the transepidermal water evaporation. Relative values were calculated assuming that the transepidermal water loss before the use test was 1, and the results are shown in Table 1.

  As shown in Table 1, the growth ratio of Staphylococcus epidermidis is synergistic by applying the three components in combination, as compared with the case of applying alone (the amount of each active ingredient is tripled). Had improved.

  As shown in Table 1, when the three components are applied in combination, the skin surface horny layer water content is synergistic as compared with the case where the three components are applied alone (the amount of each active ingredient is tripled). Had improved.

  As shown in Table 1, the transepidermal water loss was synergistically achieved by applying the three components in combination, as compared to the case of applying alone (the amount of each active ingredient was tripled). Had decreased.

Example 2 Emulsion (1) Squalane 10.0 (% by mass)
(2) Methylphenyl polysiloxane 4.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Polyoxyethylene monostearate
Sorbitan (20EO) 1.3
(6) Sorbitan monostearate 1.0
(7) Glycerin 4.0
(8) Methyl paraoxybenzoate 0.1
(9) Carboxyvinyl polymer 0.15
(10) 100 sets of purified water (11) Arginine (1% by mass aqueous solution) 20.0
(12) Dead cells of lactic acid bacteria 0.1
(13) Oligosaccharide 0.2
(14) Nigari 0.8
Production method: The oil phase components (1) to (6) are dissolved by heating at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added thereto with stirring, and the mixture is uniformly emulsified by a homogenizer. After the emulsification is completed, cooling is started, and (11) to (14) are sequentially added and uniformly mixed.

[Example 3] Lotion (1) Ethanol 15.0 (% by mass)
(2) Polyoxyethylene (40EO) hydrogenated castor oil 0.3
(3) Fragrance 0.1
(4) 100 sets of purified water (5) Citric acid 0.02
(6) Sodium citrate 0.1
(7) Glycerin 1.0
(8) Hydroxyethyl cellulose 0.1
(9) Lactic acid bacteria dead cells 0.05
(10) Oligosaccharide 0.1
(11) Bittern (from Minamidaitoshima) 1.0
Production method: (2) and (3) are dissolved in (1). After dissolution, (4) to (8) are sequentially added, and then the mixture is sufficiently stirred, and (9) to (11) are added and uniformly mixed.

Example 4 Cream (1) Squalane 10.0 (% by mass)
(2) Stearic acid 2.0
(3) Hydrogenated palm kernel oil 0.5
(4) Hydrogenated soybean phospholipid 0.1
(5) Cetanol 3.6
(6) Lipophilic glyceryl monostearate 2.0
(7) Glycerin 10.0
(8) Methyl paraoxybenzoate 0.1
(9) Arginine (20% by mass aqueous solution) 15.0
(10) Purified water 100 combinations (11) Carboxyvinyl polymer (1% by mass aqueous solution) 15.0
(12) Dead lactic acid bacteria 0.3
(13) Oligosaccharide 0.8
(14) Bittern 0.5
Production method: The oil phase components (1) to (6) are dissolved by heating at 80 ° C. On the other hand, the aqueous phase components (7) to (10) are heated and dissolved at 80 ° C. The oil phase component is added thereto with stirring, and the mixture is uniformly emulsified by a homogenizer. After the emulsification is completed, (11) is added, cooling is started, and (12) to (14) are added at 40 ° C. and mixed uniformly.

[Example 5] Serum (1) 100 purified water (% by mass)
(2) Glycerin 10.0
(3) Sucrose fatty acid ester 1.3
(4) Carboxyvinyl polymer (1% by mass aqueous solution) 17.5
(5) Sodium alginate (1% by mass aqueous solution) 15.0
(6) Polyglyceryl monolaurate 1.0
(7) Macadamia nut oil fatty acid phytosteryl 3.0
(8) N-lauroyl-L-glutamic acid di (phytosteryl-2-octyldodecyl) 2.0
(9) Hardened palm oil 2.0
(10) Squalane (derived from olives) 1.0
(11) Behenyl alcohol 0.75
(12) Beeswax 1.0
(13) Jojoba oil 1.0
(14) 1,3-butylene glycol 10.0
(15) L-arginine (10% by mass aqueous solution) 2.0
(16) Killed cells of lactic acid bacteria 0.4
(17) Oligosaccharide 1.0
(18) Nigari 1.2
Production method: The aqueous phase components (1) to (6) are mixed and heated and dissolved at 75 ° C. On the other hand, the oil phase components (7) to (14) are mixed and heated and dissolved at 75 ° C. Subsequently, after preliminarily emulsifying by adding an oil phase component to the water phase component, the mixture is uniformly emulsified by a homomixer. After completion of the emulsification, cooling is started, and (15) is added at 50 ° C. Further cool to 40 ° C., add (16) to (18), and mix uniformly.

Example 6 Aqueous Gel (1) Carboxyvinyl Polymer 0.5 (% by mass)
(2) Purified water 100 combinations (3) Sodium hydroxide (10% by mass aqueous solution) 0.5
(4) Ethanol 10.0
(5) Methyl paraoxybenzoate 0.1
(6) Fragrance 0.1
(7) Lactic acid bacteria dead cells 0.05
(8) Oligosaccharide 0.05
(9) Nigari (from Minamidaitoshima) 0.5
(10) Polyoxyethylene (60EO) hydrogenated castor oil 0.1
Production method: (1) is added to (2), and after stirring uniformly, (3) is added. After stirring uniformly, (5) previously dissolved in (4) is added. After stirring uniformly, (6) to (10) previously mixed are added, and the mixture is stirred and mixed uniformly.

Example 7 Cleansing Charge (1) Squalane 77.0 (% by mass)
(2) Polyoxyethylene glyceryl isostearate 15.0
(3) 100 sets of purified water (4) Killed cells of lactic acid bacteria 0.05
(5) Oligosaccharide 0.8
(6) Bittern (from Minamidaitoshima) 2.0
Production method: (1) and (2) are uniformly dissolved. (3) to (6) are sequentially added thereto, and the mixture is uniformly mixed.

[Example 8] Facial cleansing foam (1) Stearic acid 16.0 (% by mass)
(2) myristic acid 16.0
(3) Lipophilic glyceryl monostearate 2.0
(4) Glycerin 20.0
(5) Sodium hydroxide 7.5
(6) Coconut oil fatty acid amidopropyl betaine 1.0
(7) 100 pieces of purified water (8) Killed cells of lactic acid bacteria 0.3
(9) Oligosaccharide 0.7
(10) Nigari 2.5
Production method: The oil phase components (1) to (4) are heated and dissolved at 80 ° C. On the other hand, the aqueous phase components (5) to (7) are heated and dissolved at 80 ° C., and uniformly mixed and stirred with the oil phase components. Start cooling, add (8) to (10) at 40 ° C, and mix uniformly.

Example 9 Makeup Base Cream (1) Squalane 10.2 (% by mass)
(2) Cetanol 2.0
(3) Glycerin tri-2-ethylhexanoate 2.5
(4) Lipophilic glyceryl monostearate 1.0
(5) Propylene glycol 11.0
(6) Sucrose fatty acid ester 1.3
(7) Purified water 100 sets (8) Titanium oxide 1.0
(9) Bengala 0.1
(10) Yellow iron oxide 0.4
(11) Fragrance 0.1
(12) Dead cells of lactic acid bacteria 0.1
(13) Oligosaccharide 0.05
(14) Nigari 0.8
Production method: The oil phase components (1) to (4) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (5) to (7) are mixed and dissolved by heating at 75 ° C., and the pigments (8) to (10) are added thereto and uniformly dispersed by a homomixer. The oil phase component is added to the water phase component and emulsified by a homomixer. After the completion of the emulsification, cooling is started, and the components (11) to (14) are added at 40 ° C. and uniformly mixed.

Example 10 Emulsion Foundation (1) Methylpolysiloxane 2.0 (% by mass)
(2) Squalane 5.0
(3) Octyldodecyl myristate 5.0
(4) Cetanol 1.0
(5) Polyoxyethylene (20EO)
Sorbitan monostearate 1.3
(6) Sorbitan monostearate 0.7
(7) 1,3-butylene glycol 8.0
(8) Xanthan gum 0.1
(9) Methyl paraoxybenzoate 0.1
(10) Purified water 100 sets (11) Titanium oxide 9.0
(12) Talc 7.4
(13) Bengala 0.5
(14) Yellow iron oxide 1.1
(15) Black iron oxide 0.1
(16) Fragrance 0.1
(17) Killed cells of lactic acid bacteria 0.05
(18) Oligosaccharide 0.1
(19) Nigari (from Minamidaitoshima) 1.0
Production method: The oil phase components (1) to (6) are mixed and dissolved by heating at 75 ° C. On the other hand, the aqueous phase components (7) to (10) are mixed and dissolved by heating at 75 ° C., and the pigments (11) to (15) are added thereto and uniformly dispersed by a homomixer. Add oil phase components and emulsify. After the completion of the emulsification, cooling is started, and the components (16) to (19) are sequentially added at 40 ° C. and uniformly mixed.

[Example 11] Water-in-oil type emollient cream (1) Liquid paraffin 30.0 (% by mass)
(2) Microcrystalline wax 2.0
(3) Vaseline 5.0
(4) Diglycerin oleate 5.0
(5) Nigari 1.4
(6) Propylene glycol 3.0
(7) 1,3-butylene glycol 5.0
(8) Methyl paraoxybenzoate 0.1
(9) Killed lactic acid bacteria 0.5
(10) Oligosaccharide 0.8
(11) Purified water 100 sets (12) Fragrance 0.1
Production method: (5) is dissolved in a part of (11) to 50 ° C., and gradually added to (4) heated to 50 ° C. with stirring. After mixing, the mixture is heated and dissolved at 70 ° C. and uniformly dispersed in (1) to (3). A mixture obtained by heating and dissolving (6) to (10) in the remainder of (11) at 70 ° C. is added thereto with stirring, and emulsified by a homomixer. After completion of the emulsification, cooling is started, (12) is added at 40 ° C., and the mixture is uniformly mixed.

[Example 12] Pack (1) 100 purified water (% by mass)
(2) Polyvinyl alcohol 12.0
(3) Ethanol 17.0
(4) Glycerin 5.0
(5) polyethylene glycol (average molecular weight 1000) 2.0
(6) Killed cells of lactic acid bacteria 0.08
(7) Oligosaccharide 1.0
(8) Bittern (from Minamidaitoshima) 0.5
(9) Fragrance 0.1
Production method: (2) and (3) are mixed, heated to 80 ° C., and dissolved in (1) heated to 80 ° C. After uniform dissolution, (4) and (5) are added, and cooling is started with stirring. Cool to 40 ° C., add (6) to (9), and mix uniformly.

Claims (1)

An external preparation for skin containing the following (A) to (C).
(A) Dead cells of lactic acid bacteria (B) Oligosaccharides (C) Bittern

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