JP2020033331A - Fabaceae plant growth promoter - Google Patents

Abstract

To provide a component that promotes the growth of Fabaceae plants.SOLUTION: A Fabaceae plant growth promoter has soya saponin and a nonionic surfactant with an HLB of 9-20 as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a component that promotes the growth of legumes.

  BACKGROUND ART Legumes such as soybeans are important plant resources that are widely cultivated and used worldwide as raw materials for food, feed, oils and fats. Various types of legumes are eaten around the world. Increasing the yield of these legumes is an important issue.

  Saponins are a kind of glycosides contained in various plants, and have been used as surfactants, emulsifiers, and the like. The structures of saponins are roughly classified into triterpenoid saponins and steroid saponins, and the types are extremely diverse. The diverse structures of saponins reflect their diverse biological activities. That is, saponins exert various physiological effects on animals and plants, and their effects seem to differ depending on the structure. For example, it has been reported that Kiraya saponin (Patent Literature 1) and saponin extracted from Luffa, Panax ginseng, cucumber, melon, or Amachadium (Patent Literature 2) promote plant growth or increase yield. In addition, it is described that saponins derived from tea seeds promote infection of plants with VA mycorrhizal fungi (Vesicular Arbuscular Mycorrhizae), which promotes plant growth (Patent Document 3). Also described are plant growth regulators such as vegetables belonging to the Compositae, Solanaceae, Brassicaceae, Cruciferae, Cucurbitaceae, Lily family and Apiaceae family that contain tea seed saponin as an active ingredient (Patent Document 4). .

  Soyasaponins are a kind of saponins belonging to the oleanane-type triterpenoid saponins, and are characteristic metabolites contained in legumes. Non-Patent Document 1 describes that DDMP saponin, one of soyasaponins, was involved in removing active oxygen and promoting root elongation in germinated soybeans. Non-Patent Document 2 describes that crude saponin derived from mungbean promoted the germination rate and early growth of mungbean.

  Patent Document 5 discloses that tea saponin and quillaja saponin are used as solubilizers for solubilizing Hiba oil or an acidic oil thereof in a plant growth promoter containing Hiba oil or an acidic oil prepared from the Hiba oil as an active ingredient. And the use of saponins such as soybean saponins and nonionic or anionic surfactants. Regarding the effect of a surfactant on plant growth, for example, Non-Patent Document 3 discloses that a nonionic surfactant Tween 20 (polyoxyethylene sorbitan monolaurate) promotes root elongation on barley seedlings. It has been reported that cucumber seedlings inhibit elongation of the main root. It is also described that another nonionic surfactant, SteroxCD (polyoxyethylene ester), inhibits root elongation in both barley and cucumber seedlings.

JP 2004-121186 A JP-A-61-15806 JP-A-8-23963 JP 2000-119118 A JP 2001-192212 A

Seed Science and Biotechnology, Seed Physiological and Biochemical Research Group (edited), Academic Press, 2009, pp.106-112 Botanical Bulletin of Academia Sinica, 1995, 36 (1): 9-18 Plant Physiology, 1964, 39 (3): 502-507.

  There is a need for more efficient production of legumes, which are important crops, especially for higher yields. The present invention provides a component that promotes the growth of legumes.

  The present inventors have found that the combined use of soyasaponin and a nonionic surfactant promotes the growth of legumes.

Therefore, in one embodiment, the present invention provides a leguminous plant growth promoter comprising soyasaponin and HLB 9-20 nonionic surfactant as active ingredients.
In a further embodiment, the present invention provides a method for promoting the growth of legumes using soyasaponin and a nonionic surfactant of HLB 9-20 as active ingredients.

  ADVANTAGE OF THE INVENTION According to this invention, growth of leguminous plants can be promoted and the productivity of leguminous plants which are important crops can be improved.

The underground dry weight of soybean to which soyasaponin or a surfactant was applied, which was measured in Test 1. The underground dry weight of soybean measured in test 2. The underground dry weight of soybean measured in test 3.

  In the present invention, soyasaponin and a nonionic surfactant are used in combination as active ingredients for promoting the growth of legumes.

  As used herein, the term "promotion of growth" of legumes refers to, for example, an increase in the weight of the above-ground part and the lower part of the legume, an increase in the number of lateral buds, an increase in yield, and promotion of nodulation (for example, Increase or nodule weight increase). As used herein, the term "increase in yield" refers to a group consisting of an increase in the number of flowers, the number of pods, the weight of pods, the weight of each seed, and the total weight of seeds per plant or unit area (so-called seed yield). One or more selected from the following. In soybean, it has been reported that there is a significant correlation between root weight and yield among individuals within the same cultivar (Japanese Society of Breeding and Hokkaido Cultural Studies, 1992, (31): 64). Increasing the root weight, that is, the underground weight, not only directly increases the nutrient supply capacity (source capacity), but also contributes to the prevention of lodging of the above-ground part, so that the yield of legumes can be efficiently reduced. It is expected as a way to increase. In addition, nodules are known as an underground source (nitrogen supply source) characteristic of legumes. In soybean, it has been shown that an increase in the number and weight of nodules leads to an increase in yield (Crop Research Institute Research Report, 2007, (8): 49-108). Techniques that promote nodule formation are expected to be able to achieve increased sales while suppressing the use of chemical fertilizers, and are therefore also desirable from the perspective of establishing a sustainable agricultural production system. Therefore, the term “promotion of growth” of legumes in the present specification is preferably any one selected from the group consisting of an increase in underground weight, an increase in yield, and promotion of nodulation in legumes. This means the increase in the underground weight of legumes.

  In the present specification, the “aboveground part” and the “underground part” of the leguminous plant are each higher than the upper surface of the cultivation base material (eg, soil, water, nutrient solution, medium, etc.) in the plant of the leguminous plant. Refers to the upper and lower parts.

  Examples of the “legumes” include soybean (Glycine) genus plants, common bean (Phaseolus) genus plants, chickpea (Cicer) genus plants, pea (Pisum) genus plants, lentil (Lens) genus plants, and legume (Cajanus) genus. Plants, plants of the genus Vicia, plants of the genus Arachis, plants of the genus Medicago, plants of the genus Neptunia, plants of the genus Trigonella, plants of the genus Psphocarpus, and the like. Preferred examples include soybean plants, haricot plants, chickpea plants, pea plants, lentil plants, pigeonpea plants, fava beans plants, and peanut plants, more preferred examples and Te is soybean plant of the genus Phaseolus plants, chickpea plants of the genus, and pea plants of the genus, and examples of the more preferred examples include soybean plants of the genus.

  Examples of soybean plants include soybean (Glycine max), examples of common bean plants include common beans (Phaseolus vulgaris), examples of chickpea plants include chickpeas (Cicer arietinum), and examples of pea plants Peas (bean seedlings) (Pisum sativum), Lentil (Lens culinaris) as an example of Lentil plants, Lentil (Cajanus cajan) as an example of Lentil plants, As examples of peanut plants, Vicia faba, Peanut plants, Peanuts (Arachis hypogaea), and Astragalus plants, Astragalus (Alfalfa) (Medicago sativa), As an example of Magnolia spp. For example, fenugreek (Neptunia oleracea) is an example of a fenugreek plant, and fenugreek (Trigonella foenum-graecum) is an example. Examples of winged bean plants of the genus, winged bean (Psophocarpus tetragonolobus) may be mentioned, respectively.

  In a preferred embodiment, the leguminous plants to be promoted by the present invention are soybean plants, kidney beans plants, chickpeas plants, pea plants, lentil plants, legumes plants, broad beans plants, and peanut plants. At least one selected from the group consisting of: In a more preferred embodiment, the leguminous plant to be promoted by the present invention is at least one selected from the group consisting of soybean, kidney bean, chickpea, pea, lentil, pigeon pea, faba bean, and peanut. More preferably, the leguminous plant to be promoted by the present invention is at least one selected from the group consisting of soybean, kidney bean, and chickpea, and more preferably soybean.

  Examples of soyasaponins used as the active ingredient in the present invention include glycosides using soyasapogenol A as aglycone (so-called soyasapogenol A glycoside) and glycosides using soyasapogenol B as aglycone (so-called soyasapogenol B glycoside). No. Of these, soyasapogenol B glycoside is preferred. Examples of soyasapogenol A glycosides include soyasaponin A1 and soyasaponin A2. Examples of soyasapogenol B glycosides include glycosides in which the C-22 position of soyasapogenol B is a hydroxy group and a sugar is bonded to the C-3 position hydroxy group of the soyasapogenol B (so-called group B group). Soyasapogenol) and soyasapogenol B, a sugar is bonded to the C-3 hydroxy group, and 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran- is bonded to the C-22 hydroxy group of the soyasapogenol B. Glycosides to which 4-one is bound (so-called DDMP saponins) and the like are listed, and among them, soyasaponins of Group B group are preferable. The above-mentioned soyasaponins can be used alone or in combination of two or more.

  In the present specification, the soyasaponin of the group B group refers to a soyasapogenol B represented by the following formula (I) in which the C-22 position of the soyasapogenol B is a hydroxy group, and a sugar is bonded to the C-3 position hydroxy group of the soyasapogenol B It is a glycoside of soyasapogenol B. In the present specification, DDMP saponin in which 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one is bonded to the hydroxy group at position C-22 of soyasapogenol B is soyasaponin belonging to Group B. Is not classified.

  In the formula (I), R represents a sugar residue or a sugar chain.

  In the formula (I), examples of the sugar residue represented by R include glucuronic acid, galactose, glucose, rhamnose, and arabinose, and examples of the sugar chain represented by R include rhamnose (1 → 2) Galactose (1 → 2) glucuronic acid (1 → 3), rhamnose (1 → 2) arabinose (1 → 2) glucuronic acid (1 → 3), glucose (1 → 2) galactose (1 → 2) glucuronic acid (1 → 3).

  Examples of the compound represented by the formula (I) include preferably soyasaponin Bb (also referred to as soyasaponin I), soyasaponin Bc (also referred to as soyasaponin II) and soyasaponin Ba (also referred to as soyasaponin V), and more preferably soyasaponin Bb. Is mentioned. The structures of soyasaponin Bb, soyasaponin Bc and soyasaponin Ba are represented by the following formulas (II), (III) and (IV), respectively.

  Therefore, the soyasaponol used in the present invention is preferably a soyasapogenol B glycoside wherein the C-22 position of the soyasapogenol B is a hydroxy group and the sugar is bonded to the C-3 position hydroxy group of the soyasapogenol B. (That is, soyasaponin of group B), preferably one or more selected from the group consisting of the compounds represented by the above formula (I), and more preferably a group consisting of soyasaponin Bb, soyasaponin Bc and soyasaponin Ba At least one selected from the group consisting of soyasaponin Bb.

  Soyasaponin can be prepared by extracting or purifying from legumes such as soybean, or secreting and producing from legumes such as soybean. For example, when extracting or purifying from legumes, soyasaponin is obtained by crushing legume seeds such as dried soybeans, extracting with a solvent such as ethanol, and further purifying through column or resin if necessary. Can be isolated. Further, for example, when secretory production is performed from legumes, soyasaponin secreted in a hydroponic culture solution of legumes such as soybean can be used. Alternatively, a commercially available soyasaponin (eg, available from ChromaDex, Inc.) or a soybean saponin formulation can be used. Among the commercially available soybean saponin preparations, those containing abundant soyasapogenol B glycosides and soyasaponins of the group B group are preferred. Commercially available soybean saponin preparations rich in soyasaponin of Group B are available, for example, from Wako Pure Chemical Industries, Ltd., Access One, J-Oil Mills, Fuji Oil Co., Ltd., Tokiwa Co., Ltd. It is available from the Institute for Plant Chemistry, FAP Japan, and the like.

Examples of the nonionic surfactant used as an active ingredient in the present invention include polyoxyethylene alkyl ether, polyoxyalkylene derivative, polyoxyethylene sorbitan fatty acid ester, polyoxyethylene sorbitol fatty acid ester, polyoxyethylene fatty acid ester And the like.
Examples of the polyoxyethylene alkyl ether include polyoxyethylene (C11-20) alkyl ethers having an average EO addition mole number of 1 to 50, and more preferably polyoxyethylenes having an average EO addition mole number of 3 to 20 ( C11-15) alkyl ethers.
Examples of the polyoxyalkylene derivative include a polyoxyethylene alkylene alkyl ether having an average EO addition mole number of 1 to 20, a polyoxyethylene distyrenated phenyl ether having an average EO addition mole number of 1 to 20, and an average EO addition mole number of 1 To 20 polyoxyethylene tribenzyl phenyl ethers.
Examples of the polyoxyethylene sorbitan fatty acid ester include polyoxyethylene sorbitan mono (C12-18) fatty acid ester having an average EO addition mole number of 10 to 30 and polyoxyethylene sorbitan tri (C12) having an average EO addition mole number of 10 to 30. 18) Fatty acid esters, such as polyoxyethylene sorbitan coconut oil fatty acid ester having an average EO addition mole number of 10 to 30 and polyoxyethylene sorbitan monolaurate having an average EO addition mole number of 10 to 30 are preferable.
Examples of the polyoxyethylene sorbitol fatty acid ester include polyoxyethylene sorbite tetraoleate having an average number of moles of added EO of 10 to 30.
Examples of the polyoxyethylene fatty acid ester include polyoxyethylene glycol mono (C12-18) fatty acid ester having an average EO addition mole number of 10-30, and polyoxyethylene glycol di (C12-18) having an average EO addition mole number of 10-30. ) Fatty acid esters and polyoxyethylene hydrogenated castor oil having an average number of moles of added EO of 10 to 30.
The nonionic surfactant used as an active ingredient in the present invention described above may be HLB of griffin (hereinafter, simply referred to as HLB) 9 to 20, and preferably HLB 10 to 19.
In the present invention, the above-mentioned nonionic surfactants can be used alone or in combination of two or more.

  The nonionic surfactants listed above are commercially available. For example, an emulgen (registered trademark) series is used as a polyoxyethylene alkyl ether, and an emulgen (registered trademark) LS series, an emulgen (registered trademark) MS series, and an emulgen (registered trademark) A series are used as polyoxyalkylene derivatives. The Reodol (registered trademark) series and the Reodol (registered trademark) TW series are commercially available as sorbitol fatty acid esters and polyoxyethylene sorbitan fatty acid esters, and the Emanone (registered trademark) series is commercially available as polyoxyethylene fatty acid esters. These commercially available surfactants are available from Kao Corporation.

  In a preferred embodiment, the nonionic surfactants used as active ingredients in the present invention are polyoxyethylene alkyl ethers of HLB 9-20, preferably HLB 10-19, and polyoxyethylene alkyl ethers of HLB 9-20, preferably HLB 10-19 It is at least one selected from the group consisting of oxyethylene sorbitan fatty acid esters. In another preferred embodiment, the nonionic surfactant used as an active ingredient in the present invention is a polyoxyethylene alkyl ether of HLB 9-20, preferably HLB 10-19, or HLB 9-20, preferably HLB 10-19. Is a polyoxyethylene sorbitan fatty acid ester. As the polyoxyethylene alkyl ether, polyoxyethylene (1 to 50) C11 to 20 alkyl ether is preferable, polyoxyethylene (3 to 20) C11 to 15 alkyl ether is more preferable, and polyoxyethylene (7) C11 to 15 alkyl ethers are more preferable, and as the polyoxyethylene sorbitan fatty acid ester, polyoxyethylene (10-30) sorbitan mono C12-18 fatty acid ester is preferable, and polyoxyethylene (10-30) sorbitan coconut oil fatty acid ester is more preferable. Preferably, polyoxyethylene (20) sorbitan monolaurate is more preferable (the number in parentheses represents the average number of moles of EO added). In a further preferred embodiment, the nonionic surfactant used as an active ingredient in the present invention is polyoxyethylene (7) C11-15 alkyl ether (for example, Emulgen (registered trademark) 707; HLB 12.1), polyoxyethylene (7). Ethylene (20) sorbitan monolaurate (for example, Rheodol® TW-L120; HLB 16.7), or a combination thereof.

  In the present invention, the soyasaponin and the nonionic surfactant may be further used in combination with a microbial material. The microbial material is preferably a rhizobial material. The species of rhizobia contained in the rhizobial material can be selected according to the legume to be cultivated. Examples of rhizobia and legumes to be included in the rhizobial agent used in the present invention are shown in Table 1 below. In this specification, the microorganisms mixed in the microorganism material are also referred to as microbial cells.

One of the above rhizobial species may be used alone for each target legume, or two or more of them may be used in combination. For example, among the rhizobia listed above,
For soybean plants, at least one selected from the group consisting of Bradyrhizobium and Ensifer (Sinorhizobium) is preferable, and Bradyrhizobium is more preferable. As the genus Bradyrhizobium, one or more selected from the group consisting of Bradyrhizobium japonicum, Bradyrhizobium diazoefficiens and Bradyrhizobium elkanii is preferable, and one or more selected from the group consisting of Bradyrhizobium japonicum and Bradyrhizobium diazoefficiens is more preferable. As the genus Ensifer (Sinorhizobium), Ensifer (Sinorhizobium) fredii is preferable;
For the plants of the genus Bean, the genus of Pea, the genus of Vicia, the genus of lentil, and the genus of legume, the genus Rhizobium is preferred. As the genus Rhizobium, one or more selected from the group consisting of Rhizobium leguminosarum, Rhizobium gallicum and Rhizobium giardinii is preferable, and Rhizobium leguminosarum is more preferable;
-For chickpea plants, the genus Mesorhizobium is preferable, and as the genus Mesorhizobium, one or more selected from the group consisting of Mesorhizobium ciceri and Mesorhizobium mediterraneum is preferable, and Mesorhizobium ciceri is more preferable;
-For peanut plants, Bradyrhizobium species are preferred. Preferred species of Bradyrhizobium species are the same as in the case of soybean plants.

  Alternatively, as the microorganism material, a material of a microorganism species other than rhizobia may be used, or a material combining a microorganism species other than rhizobia and a rhizobia may be used. Microorganism species other than rhizobia include, for example, plant growth promoting rhizosphere bacteria and plant growth promoting fungi. Examples of the plant growth-promoting rhizosphere bacteria include, for example, Bacillus, Pseudomonas, Azospirillum, Burkholderia, Enterobacter, Talaromyces, Arthrobacter, Agrobacterium, Corynebacterium, Erwinia Fungi, Psychrobacter spp., Serratia spp. And Rhodococcus spp .; Examples of the plant growth promoting fungi include Penicillium spp., Trichoderma sp., Fusarium sp., Phoma sp., Glomus sp., Acaulospora sp. , Entrophospora, Gigaspora, Scutellospora and Aspergillus.

  In the present invention, soyasaponin and the nonionic surfactant may be used in combination with other components for promoting the growth of legumes. Other components for promoting leguminous plant growth include, for example, plant essential nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydric alcohols, food additives, and microbial extracts Plant hormones, nod factors or lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitin compounds, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, caliquine, acyl-homoserine lactone derivatives, betaine compounds, One or more selected from phenol compounds, catechins, iron phosphate, and the like.

The essential nutrients of the plant include, for example, nitrogen, phosphorus, potassium, calcium, sulfur, magnesium, boron, chlorine, manganese, iron, zinc, copper, molybdenum, nickel and the like; and the flavonoids include, for example, genistin, daidzin , Genistein, daidzein and the like; examples of the organic acid include citric acid, oxalic acid, ferulic acid, caffeic acid, malic acid, malonic acid, picidic acid, mugineic acid, dehydroxymugineic acid, hydroxymugineic acid, and acetic acid Such amino acids include, for example, serine, proline, leucine, isoleucine, methionine, cysteine, tryptophan, asparagine, glutamine, aspartic acid, glutamic acid, 5-aminolevulinic acid; and the peptide; Gluta for example Nucleosides, glycopeptides, soybean protein degradation products, plant-derived polypeptides, and the like; the nucleosides include, for example, inosine, guanosine, and uridine; the nucleotides include, for example, inosine, guanylic acid, and uridylic acid The nucleobase includes, for example, hypoxanthine, guanine, uracil and the like; the saccharide includes, for example, trehalose, sucrose, glucose, maltose; and the monohydric alcohol includes, for example, alcohol lauryl Alcohol, cetyl alcohol, stearyl alcohol, oleyl alcohol and the like; the food additive includes, for example, chitosan; the microbial extract includes, for example, yeast extract; For example, indole-3-acetic acid, indole-3-butyric acid, naphthalene acetic acid, naphthoxyacetic acid, phenylacetic acid, 2,4-dichlorophenoxyacetic acid, 2,4,5-trichlorophenoxyacetic acid, zeatin, kinetin, benzyladenine, thidiazuron, gibberellin , Strigolactone, jasmonic acid, methyl jasmonate, and the like; examples of the nod factor, that is, lipo-chitooligosaccharide, include NodRM, NodRM-1, NodRM-3, and US Patent Nos. 5,175,149 and 5, , 321, 011, BjNod-V (Ac, C18: 1), BjNod-V (C16: 1) and BjNod-V (Ac, C16: 0), And My2010 described in WO2010 / 049751 Examples of the synthetic lipo-chitooligosaccharide include the synthetic LCO compound described in WO2005 / 063784, and AcNodRM-1 and AcNodRM- described in US Pat. No. 5,545,718. 3 and the like; the chitooligosaccharides include, for example, oligo-N-acetylglucosamine; the chitin compounds include chitin and chitosan; the linoleic acid and derivatives thereof include Examples of the linolenic acid or derivatives thereof include, for example, linolenic acid; examples of the calyquine include hydrochloride and hydrobromide; examples of the acyl-homoserine lactone derivative include For example, N-acyl-L Homoserine lactone, N-hexanoyl-L-homoserine lactone, N- (3-oxooctanoyl) -L-homoserine lactone and the like; examples of the betaine compound include N, N, N-trimethylglycine, carnitine and the like. Examples of the phenol compounds include cresol and chlorophenol; examples of the catechins include catechin (C), gallocatechin (GC), catechin gallate (Cg), gallocatechin gallate (GCg), Catechin (EC), epigallocatechin (EGC), epicatechin gallate (ECg), and epigallocatechin gallate (EGCg); such iron phosphates include iron (III) phosphate (FePO ₄ ) Can be

  In the present invention, the microbial material or other components may be contained in a composition containing soyasaponin and / or a nonionic surfactant described below, or separately from the soyasaponin and the nonionic surfactant. It may be applied to legumes. The microbial material or other component may be used in an amount that does not inhibit the legume plant growth promoting effect of soyasaponin and the nonionic surfactant.

  In the present invention, the soyasaponin and the nonionic surfactant may be used in any form as long as they are used in combination with plants. For example, soyasaponin and a nonionic surfactant may each be applied to a plant alone, or a composition comprising soyasaponin or a nonionic surfactant may be applied to a plant. Preferably, a composition comprising soyasaponin and a non-ionic surfactant is applied to the plant. In the following specification, a composition containing the soyasaponin and a nonionic surfactant is referred to as a legume plant growth promotion composition (or simply a growth promotion composition) of the present invention. The composition for promoting growth may include two separate components consisting of a composition containing soyasaponin and a composition containing a nonionic surfactant, and one composition containing soyasaponin and a nonionic surfactant. It may be a composition. Therefore, in the present invention, the composition containing soyasaponin and the composition containing a nonionic surfactant may be separately applied to plants, or one composition containing soyasaponin and a nonionic surfactant. The object may be applied to plants. In the present invention, the soyasaponin and the nonionic surfactant can each have an arbitrary form such as a block, a powder, a granule, a liquid, and a gel. In the present invention, soyasaponin and the nonionic surfactant can be provided as agricultural or horticultural materials.

  When using soyasaponin or a nonionic surfactant alone, add soyasaponin or a nonionic surfactant to a cultivation base material for cultivating legumes, such as soil, a medium, and a solution for hydroponics. Alternatively, water or an additive containing soyasaponin or a nonionic surfactant may be prepared, and the water or the additive may be added to the cultivation base material for legumes. Alternatively, water or an additive containing soyasaponin or a nonionic surfactant may be prepared, and the water or the additive may be provided to a seed or a plant of a leguminous plant.

  Examples of the leguminous plant growth-promoting composition of the present invention include, for example, cultivation bases containing soyasaponin and a nonionic surfactant (for example, agricultural or horticultural soil, cultivated soil, medium, Solution, water, etc.), fertilizers, water for watering, microbial materials such as rhizobial materials, soil conditioners, pesticides, seeding materials, plant supplements (eg, activators, nutrients, etc.), and the like. But not limited to these.

  Fertilizers, microbial materials, soil improvers, sowing materials, and plant supplements containing soyasaponin and a nonionic surfactant are preferred because they contribute to the improvement of soil for cultivating legumes. The fertilizer, microbial material, soil conditioner, sowing material, and plant supplement may be solid or liquid. When the fertilizer, microbial material, soil conditioner, seeding material, or plant supplement is solid, it can be in the form of a block, powder, granules, etc., but is preferably powder or granules. The fertilizer, microbial material, soil conditioner, sowing material, and plant supplement include fertilizer, microbial material, soil conditioner usually used for cultivation of leguminous plants, in addition to containing soyasaponin and a nonionic surfactant. It can include ingredients, sowing materials, and plant supplement components.

  Cultivation base materials, fertilizers, microbial materials such as rhizobial materials, soil conditioners, agrochemicals, seeding materials, and plant supplements containing the soyasaponin and nonionic surfactants can be obtained from ordinary cultivation base materials (eg, Or horticultural soil, cultivation soil, medium, nutrient solution, water, etc.), fertilizers, microbial materials such as rhizobial materials, soil improvers, pesticides, seeding materials, plant supplements (eg, activators) , Nutrients, etc.) and soyasaponin and a nonionic surfactant.

  The concentration of soyasaponin in the composition for promoting growth of the present invention may be, for example, preferably 0.1% in the total amount of the composition when the composition is a fertilizer, a microbial material, a soil conditioner, a sowing material, a plant supplement, or the like. 0005% by mass or more, more preferably 0.005% by mass or more, and preferably 80% by mass or less, more preferably 50% by mass or less, still more preferably 5% by mass or less, and still more preferably 0.5% by mass. Or 0.005 to 80% by mass, more preferably 0.0005 to 50% by mass, 0.0005 to 5% by mass, 0.0005 to 0.5% by mass in the total amount of the composition. % By mass, 0.005 to 80% by mass, 0.005 to 50% by mass, 0.005 to 5% by mass or 0.005 to 0.5% by mass. Alternatively, when the composition is a cultivation base material, the concentration of soyasaponin in the composition is preferably 0.01 mass ppm or more, more preferably 0.1 mass ppm or more, in the total amount of the composition, And preferably 100 ppm by mass or less, more preferably 10 ppm by mass or less, still more preferably 5 ppm by mass or less, still more preferably 2 ppm by mass or less, or in the total amount of the composition, preferably 0.01 to 100 mass ppm, more preferably 0.01 to 10 mass ppm, 0.01 to 5 mass ppm, 0.01 to 2 mass ppm, 0.1 to 100 mass ppm, 0.1 to 10 mass ppm, 0.1 -5 mass ppm or 0.1-2 mass ppm.

  The concentration of the nonionic surfactant in the composition for promoting growth of the present invention is, for example, when the composition is a fertilizer, a microbial material, a soil conditioner, a seeding material, a plant supplement, or the like, in the total amount of the composition. , Preferably 0.0005% by mass or more, more preferably 0.005% by mass or more, and preferably 80% by mass or less, more preferably 50% by mass or less, still more preferably 5% by mass or less, further preferably 0.5% by mass or less, or preferably 0.0005 to 80% by mass, more preferably 0.0005 to 50% by mass, 0.0005 to 5% by mass, and 0. 0005 to 0.5% by mass, 0.005 to 80% by mass, 0.005 to 50% by mass, 0.005 to 5% by mass or 0.005 to 0.5% by mass. Alternatively, when the composition is a cultivation base material, the concentration of the nonionic surfactant in the composition is preferably 0.01% by mass or more, more preferably 0.1% by mass, in the total amount of the composition. Or more, preferably 100 mass ppm or less, more preferably 10 mass ppm or less, still more preferably 5 mass ppm or less, still more preferably 2 mass ppm or less, or in the total amount of the composition, Is 0.01 to 100 mass ppm, more preferably 0.01 to 10 mass ppm, 0.01 to 5 mass ppm, 0.01 to 2 mass ppm, 0.1 to 100 mass ppm, 0.1 to 10 mass ppm, 0.1 to 5 ppm by mass or 0.1 to 2 ppm by mass.

  The composition for promoting growth of the present invention can contain any of the above-mentioned concentrations of soyasaponin and any of the above-mentioned concentrations of nonionic surfactant in any combination. As an example of the ratio of the mass of the nonionic surfactant to the mass of soyasaponin, preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times, more preferably Is 0.3 to 3 times, and more preferably 0.5 to 2 times. Preferred examples of the concentration include 0.0005 to 80% by mass of soyasaponin and 0.0005 to 80% by mass of a nonionic surfactant, 0.0005 to 50% by mass of soyasaponin and 0.0005% by mass of a nonionic surfactant. 50 to 50% by mass, soyasaponin 0.0005 to 5% by mass and nonionic surfactant 0.0005 to 5% by mass, soyasaponin 0.0005 to 0.5% by mass and nonionic surfactant 0.0005 to 0% 0.5 mass%, soyasaponin 0.005 to 80 mass% and nonionic surfactant 0.005 to 80 mass%, soyasaponin 0.005 to 50 mass% and nonionic surfactant 0.005 to 50 mass% 0.005 to 5% by mass of soyasaponin and 0.005 to 5% by mass of a nonionic surfactant, and 0.005 to 0.5% by mass of soyasaponin and Etc. On surfactant 0.005% by mass can be mentioned.

  The amount of soyasaponin used for promoting the growth of legumes according to the present invention is preferably 0.01 to 100 ppm by mass, more preferably, as the concentration in the cultivation base material for cultivating the legumes as described above. Is 0.01 to 50 mass ppm, 0.01 to 20 mass ppm, 0.01 to 13 mass ppm, 0.1 to 10 mass ppm, 0.1 to 5 mass ppm or 0.1 to 2 mass ppm I just need. For example, the amount of soyasaponin used per liter of cultivation base material is preferably 0.01 to 100 mg, more preferably 0.01 to 50 mg, 0.01 to 20 mg, 0.01 to 13 mg, and 0.1 to 10 mg. , 0.1 to 5 mg or 0.1 to 2 mg. If legumes are cultivated in soil, preferably 10 to 10 kg of land, more preferably 0.001 to 10 kg, more preferably 0.001 to 5 kg, 0.001 to 2 kg, 0.001 to 1.3 kg, 0 Soyasaponin may be added to the soil in an amount of 0.01-1 kg, 0.01-0.5 kg, or 0.01-0.2 kg.

  The amount of the nonionic surfactant for promoting the growth of legumes according to the present invention is preferably 0.01 to 100 as the concentration in the cultivation base material for cultivating the legumes as described above. Mass ppm, more preferably 0.01 to 50 mass ppm, 0.01 to 20 mass ppm, 0.01 to 13 mass ppm, 0.1 to 10 mass ppm, 0.1 to 5 mass ppm or 0.1 to What is necessary is just 2 mass ppm. For example, the amount of the nonionic surfactant used per liter of cultivation base material is preferably 0.01 to 100 mg, more preferably 0.01 to 50 mg, 0.01 to 20 mg, and 0.01 to 13 mg. It may be 0.1 to 10 mg, 0.1 to 5 mg or 0.1 to 2 mg. If legumes are cultivated in soil, preferably 10 to 10 kg of land, more preferably 0.001 to 10 kg, more preferably 0.001 to 5 kg, 0.001 to 2 kg, 0.001 to 1.3 kg, 0 The nonionic surfactant may be added to the soil in an amount of 0.01-1 kg, 0.01-0.5 kg, or 0.01-0.2 kg.

  In promoting the growth of legumes according to the present invention, any of the above-mentioned amounts of soyasaponin and any of the above-mentioned nonionic surfactants can be used in any combination. Preferred examples of the amount used include, as the concentration in the cultivation base material, soyasaponin 0.01 to 100 mass ppm and a nonionic surfactant 0.01 to 100 mass ppm, soyasaponin 0.01 to 50 mass ppm and a non-ionic surfactant. 0.01-50 mass ppm of ionic surfactant, 0.01-20 mass ppm of soyasaponin and 0.01-20 mass ppm of nonionic surfactant, 0.01-13 mass ppm of soyasaponin and nonionic surfactant 0.01 to 13 mass ppm, soyasaponin 0.1 to 10 mass ppm and nonionic surfactant 0.1 to 10 mass ppm, soyasaponin 0.1 to 5 mass ppm and nonionic surfactant 0.1 -5 mass ppm, soyasaponin 0.1-2 mass ppm, and nonionic surfactant 0.1-2 mass ppm. Alternatively, as an example of a preferable amount of the legume when cultivating the leguminous plant, 0.001 to 10 kg of soyasaponin, 0.001 to 10 kg of a nonionic surfactant, and 0.001 to 5 kg of soyasaponin per 10 ares of land And 0.001 to 5 kg of nonionic surfactant, 0.001 to 2 kg of soyasaponin and 0.001 to 2 kg of nonionic surfactant, 0.001 to 1.3 kg of soyasaponin and 0.001 of nonionic surfactant 1.3 kg, soyasaponin 0.01-1 kg and nonionic surfactant 0.01-1 kg, soyasaponin 0.01-0.5 kg and nonionic surfactant 0.01-0.5 kg, and soyasaponin 0 0.01 to 0.2 kg and 0.01 to 0.2 kg of a nonionic surfactant. As an example of the ratio of the mass of the nonionic surfactant to the mass of soyasaponin in the cultivation base material, preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 times The ratio is from 10 to 10, more preferably from 0.3 to 3, and still more preferably from 0.5 to 2.

  When the growth promoting composition of the present invention is used, the amount used depends on the concentrations of soyasaponin and the nonionic surfactant contained in the composition. If the composition is a fertilizer, a microbial material, a soil conditioner, a seeding material, or a plant supplement, the concentration of soyasaponin contained in the composition is 0.005% by mass, and a nonionic surfactant is used. When the concentration is 0.005% by mass, the use amount of the composition per 10 ares of land is preferably 20 to 200,000 kg, more preferably 20 to 100,000 kg, 20 to 40,000 kg, and 20 to 26 kg. 2,000 kg, 200-20,000 kg, 200-10,000 kg or 200-4,000 kg.

  In the present invention, examples of the method of cultivating legumes include hydroponic cultivation, spray cultivation, sand cultivation, gravel cultivation and other nutrient cultivation, soil cultivation, and nutrient cultivation. Soil cultivation is preferred. For soil cultivation, it is preferable to use agricultural or horticultural soil or cultivation soil. The soil or cultivation soil used for soil cultivation is preferably obtained by performing soil improvement such as agglomeration treatment. In addition, as a soil improver for the agglomeration treatment of soil or cultivated soil, a soil improver containing an alkali-treated lignin as an active ingredient described in JP-A-2017-190448 is preferable.

  The cultivation of legumes in the present invention is preferably indoor cultivation from the viewpoint of environmental stability, but is more preferably outdoor cultivation from the viewpoint of ensuring yield. Regardless of the method used for cultivation, in the method for promoting the growth of legumes according to the present invention, soyasaponin and a nonionic surfactant are used as active ingredients. In particular, legumes to which soyasaponin and a nonionic surfactant have been applied are grown. In one embodiment of the method of the present invention, the soyasaponin and the nonionic surfactant are preferably added to a cultivation substrate (for example, soil, cultivation soil, a medium, a solution for nutrient solution, water, etc.). If legumes are cultivated by a normal procedure on a cultivation base material containing soyasaponin and a nonionic surfactant, the growth promotion effect of legumes by soyasaponin of Group B can be obtained. For example, in soil cultivation, fertilizers, rhizobial materials, and the like that are usually applied to legumes may be added to the soil, if necessary, and soyasaponin and a nonionic surfactant may be applied thereto. Alternatively, when soyasaponin and a nonionic surfactant are used in the form of a fertilizer or a microbial material, it is not necessary to separately add a fertilizer or a microbial material, but in combination with the addition of another fertilizer or a microbial material. Is also good. If legumes are cultivated in the soil to which the prepared soyasaponin and the nonionic surfactant are added by a usual procedure, the growth promoting effect of the soyasaponin and the nonionic surfactant can be obtained. In the case of hydroponics, soyasaponin and a nonionic surfactant may be added to the nutrient solution. The addition of soyasaponin and the nonionic surfactant to the cultivation base material is preferably before sowing, but may be added after sowing, or may be added both before and after sowing.

  In another embodiment of the method of the present invention, the soyasaponin or non-ionic surfactant may be provided by applying or smearing the seed before sowing (eg as a seed dressing). For example, if a seed to which soyasaponin and a nonionic surfactant are added is cultivated by a normal procedure on a cultivation base material (for example, soil, cultivation soil, a medium, a solution for nutrient solution, water, etc.), the soyasaponin and the nonionic surfactant are added. The growth promoting effect of the surfactant can be obtained. Alternatively, the seed to which soyasaponin has been added may be cultured on a cultivation substrate containing a nonionic surfactant, or the seed to which a nonionic surfactant has been added may be cultured on a cultivation substrate containing soyasaponin. . In yet another embodiment, the addition of soyasaponin or a nonionic surfactant to the cultivation base and the addition of seed to the seed before sowing so that the plant can be applied with both soyasaponin and a nonionic surfactant. It may be combined with application or smearing of soyasaponin or a nonionic surfactant.

  In a further embodiment of the method of the present invention, the soyasaponin and the nonionic surfactant may be provided by direct spraying, spraying or applying (eg, foliar spraying) to the leguminous plant. If the leguminous plant to which the soyasaponin and the nonionic surfactant are added is cultivated by a usual procedure, the growth promoting effect can be obtained. In yet another embodiment, the addition of soyasaponin and a nonionic surfactant to the above-described cultivation base material (eg, soil, cultivation soil, medium, nutrient solution, water, etc.), or seed before sowing The application or smearing of soyasaponin on the plant may be combined with the direct application, spraying or application of soyasaponin and a nonionic surfactant on the plant of the legume.

  The cultivation period of the leguminous plant according to the method of the present invention is preferably at least 20 days after seeding, and more preferably until the time when the seeds can be harvested. However, the time required until the seed can be harvested differs depending on the type of legume and the cultivation environment.

  All patents, non-patent references, and other publications cited herein are incorporated by reference in their entirety.

  As exemplary embodiments of the present invention, the following materials, manufacturing methods, applications, methods, and the like are further disclosed herein. However, the present invention is not limited to these embodiments.

[1] A leguminous plant growth promoter comprising soyasaponin and HLB 9 to 20 nonionic surfactants as active ingredients.
[2] The nonionic surfactant is:
Preferably, at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters,
More preferably, at least one selected from the group consisting of polyoxyethylene alkyl ether and polyoxyethylene sorbitan fatty acid ester,
More preferably, polyoxyethylene alkyl ether,
[1] The leguminous plant growth promoter according to [1].
[3] Preferably, the legume plant growth promoter according to [1] or [2], wherein the nonionic surfactant is HLB 10 to 19.
[4] the soyasaponin is
Preferably, it is soyasapogenol B glycoside,
More preferably, a glycoside of soyasapogenol B, wherein the C-22 position of the soyasapogenol B is a hydroxy group, and a sugar is bonded to the hydroxy group at the C-3 position of the soyasapogenol B Yes,
More preferably, it is soyasaponin Bb,
The agent for promoting the growth of legumes according to any one of [1] to [3].
[5] The legume plant is
Preferably, it is at least one selected from the group consisting of a soybean plant, a kidney bean plant, a chickpea plant, a pea plant, a lentil plant, a legume plant, a fava bean plant and a peanut plant,
More preferably, it is at least one selected from the group consisting of soybeans, kidney beans, chickpeas, peas, lentils, pigeonpeas, fava beans and peanuts.
The legume plant growth promoter according to any one of [1] to [4].
[6] Preferably, the cultivation base for legumes, pesticides, fertilizers, microbial materials, soil conditioners, seeding materials or plant supplements, according to any one of [1] to [5] Legume plant growth promoter.
[7] Preferably, microbial materials, plant essential nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydric alcohols, nonionic surfactants, food additives, microbial extracts Plant hormones, nod factors or lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitin compounds, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, caliquine, acyl-homoserine lactone derivatives, betaine compounds, The leguminous plant growth promoter according to any one of [1] to [6], further comprising at least one selected from the group consisting of phenolic compounds, catechins, and iron phosphate.
[8] Preferably, the legume plant growth promoter according to any one of [1] to [7], comprising the soyasaponin and a nonionic surfactant at the following concentrations:
0.005 to 80% by mass of soyasaponin and 0.0005 to 80% by mass of nonionic surfactant; 0.0005 to 50% by mass of soyasaponin and 0.0005 to 50% by mass of nonionic surfactant; 0.0005 of Soyasaponin 0.005 to 5% by mass of non-ionic surfactant; 0.0005 to 0.5% by mass of soyasaponin and 0.0005 to 0.5% by mass of non-ionic surfactant; 0.005% by mass of soyasaponin -80 mass% and nonionic surfactant 0.005 to 80 mass%; soyasaponin 0.005 to 50 mass% and nonionic surfactant 0.005 to 50 mass%; soyasaponin 0.005 to 5 mass% And 0.005 to 5% by mass of a nonionic surfactant; or 0.005 to 0.5% by mass of soyasaponin and 0.005 to 0% by mass of a nonionic surfactant It is 5% by mass,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[9] The leguminous plant growth promoter according to [8], which is preferably a fertilizer, a microbial material, a soil conditioner, a seeding material or a plant supplement.
[10] Preferably, the leguminous plant according to any one of [1] to [7], which is a cultivation base material for leguminous plants containing the soyasaponin and a nonionic surfactant at the following concentrations. Growth promoter:
0.01 to 100 mass ppm of soyasaponin and 0.01 to 100 ppm by mass of nonionic surfactant; 0.01 to 50 massppm of soyasaponin and 0.01 to 50 ppm by mass of nonionic surfactant; 0.01 of soyasaponin -20 mass ppm and non-ionic surfactant 0.01-20 mass ppm; soyasaponin 0.01-13 mass ppm and non-ionic surfactant 0.01-13 mass ppm; soyasaponin 0.1-10 mass ppm And 0.1 to 10 mass ppm of non-ionic surfactant; 0.1 to 5 mass ppm of soyasaponin and 0.1 to 5 mass ppm of non-ionic surfactant; or 0.1 to 2 mass ppm of soyasaponin and non-ionic 0.1 to 2 mass ppm of the ionic surfactant,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, further preferably 0.5 to 2 times.
[11] Preferably, the legume plant growth promoter according to [10], wherein the cultivation base material is soil, cultivated soil, a medium, a solution for nutrient solution cultivation or water.
[12] Preferably, the legume plant growth promoter according to any one of [1] to [11], wherein the growth promotion is an increase in underground weight.

[13] Use of a nonionic surfactant of soyasaponin and HLB 9 to 20 for producing a leguminous plant growth promoter.
[14] The nonionic surfactant is:
Preferably, at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters,
More preferably, at least one selected from the group consisting of polyoxyethylene alkyl ether and polyoxyethylene sorbitan fatty acid ester,
More preferably, polyoxyethylene alkyl ether,
[13] Use according to the above.
[15] Preferably, the use according to [13] or [14], wherein the nonionic surfactant is HLB 10 to 19.
[16] The soyasaponin is
Preferably, it is soyasapogenol B glycoside,
More preferably, a glycoside of soyasapogenol B, wherein the C-22 position of the soyasapogenol B is a hydroxy group, and a sugar is bonded to the hydroxy group at the C-3 position of the soyasapogenol B Yes,
More preferably, it is soyasaponin Bb,
Use according to any one of [13] to [15].
[17] The legume plant,
Preferably, it is at least one selected from the group consisting of a soybean plant, a kidney bean plant, a chickpea plant, a pea plant, a lentil plant, a legume plant, a fava bean plant and a peanut plant,
More preferably, it is at least one selected from the group consisting of soybeans, kidney beans, chickpeas, peas, lentils, pigeonpeas, fava beans and peanuts.
Use according to any one of [13] to [16].
[18] Preferably, the leguminous plant growth promoter is a cultivation base material for a leguminous plant, a pesticide, a fertilizer, a microbial material, a soil improving agent, a seeding material or a plant supplement, [13] to [ [17] The use according to any one of [17].
[19] Preferably, the legume plant growth promoter is a microorganism material, plant essential nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleic acid bases, sugars, monohydric alcohols, nonionic surfactants Agents, food additives, microbial extracts, plant hormones, nod factors or lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitinic compounds, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, calykin, The use according to any one of [13] to [18], further comprising at least one selected from the group consisting of an acyl-homoserine lactone derivative, a betaine compound, a phenolic compound, a catechin, and iron phosphate. .
[20] The use according to any one of [13] to [19], wherein the legume plant growth promoter contains the soyasaponin and a nonionic surfactant at the following concentrations:
0.005 to 80% by mass of soyasaponin and 0.0005 to 80% by mass of nonionic surfactant; 0.0005 to 50% by mass of soyasaponin and 0.0005 to 50% by mass of nonionic surfactant; 0.0005 of Soyasaponin 0.005 to 5% by mass of non-ionic surfactant; 0.0005 to 0.5% by mass of soyasaponin and 0.0005 to 0.5% by mass of non-ionic surfactant; 0.005% by mass of soyasaponin -80 mass% and nonionic surfactant 0.005 to 80 mass%; soyasaponin 0.005 to 50 mass% and nonionic surfactant 0.005 to 50 mass%; soyasaponin 0.005 to 5 mass% And 0.005 to 5% by mass of a nonionic surfactant; or 0.005 to 0.5% by mass of soyasaponin and 0.005 to 0% by mass of a nonionic surfactant It is 5% by mass,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[21] The use according to [20], wherein the legume plant growth promoter is preferably a fertilizer, a microbial material, a soil conditioner, a seeding material, or a plant supplement.
[22] Preferably, the leguminous plant growth promoter is a cultivation substrate for legumes containing the soyasaponin and a nonionic surfactant at the following concentrations, [13] to [19]. Use according to any one of the following:
0.01 to 100 mass ppm of soyasaponin and 0.01 to 100 ppm by mass of nonionic surfactant; 0.01 to 50 massppm of soyasaponin and 0.01 to 50 ppm by mass of nonionic surfactant; 0.01 of soyasaponin -20 mass ppm and non-ionic surfactant 0.01-20 mass ppm; soyasaponin 0.01-13 mass ppm and non-ionic surfactant 0.01-13 mass ppm; soyasaponin 0.1-10 mass ppm And 0.1 to 10 mass ppm of non-ionic surfactant; 0.1 to 5 mass ppm of soyasaponin and 0.1 to 5 mass ppm of non-ionic surfactant; or 0.1 to 2 mass ppm of soyasaponin and non-ionic 0.1 to 2 mass ppm of the ionic surfactant,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[23] The use according to [22], wherein the cultivation base material is preferably soil, cultivated soil, a medium, a solution for nutrient solution cultivation or water.
[24] The use according to any one of [13] to [23], wherein the growth promotion is an increase in underground weight.

[25] Use of a nonionic surfactant of soyasaponin and HLB 9 to 20 for promoting legume plant growth.
[26] the nonionic surfactant is:
Preferably, at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters,
More preferably, at least one selected from the group consisting of polyoxyethylene alkyl ether and polyoxyethylene sorbitan fatty acid ester,
More preferably, polyoxyethylene alkyl ether,
[25] Use according to the above.
[27] The use according to [25] or [26], wherein the nonionic surfactant is preferably HLB 10 to 19.
[28] The soyasaponin is
Preferably, it is soyasapogenol B glycoside,
More preferably, a glycoside of soyasapogenol B, wherein the C-22 position of the soyasapogenol B is a hydroxy group, and a sugar is bonded to the hydroxy group at the C-3 position of the soyasapogenol B Yes,
More preferably, it is soyasaponin Bb,
Use according to any one of [25] to [27].
[29] the legume plant,
Preferably, it is at least one selected from the group consisting of a soybean plant, a kidney bean plant, a chickpea plant, a pea plant, a lentil plant, a legume plant, a fava bean plant and a peanut plant,
More preferably, it is at least one selected from the group consisting of soybeans, kidney beans, chickpeas, peas, lentils, pigeonpeas, fava beans and peanuts.
Use according to any one of [25] to [28].
[30] Preferably, the soyasaponin and the nonionic surfactant are contained in a composition, and the composition is a cultivation base material for legumes, a pesticide, a fertilizer, a microbial material, a soil conditioner, a sowing agent, Use according to any one of [25] to [29], which is a material or a plant supplement.
[31] Preferably, the composition is a microorganism material, plant essential nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydric alcohols, nonionic surfactants, food additives Products, microbial extracts, plant hormones, nod factors, ie lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitin compounds, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, caliquine, acyl-homoserine lactone The use according to [30], further comprising one or more selected from the group consisting of derivatives, betaine compounds, phenolic compounds, catechins, and iron phosphate.
[32] Preferably, the composition according to [30] or [31], wherein the composition contains the soyasaponin and a nonionic surfactant at the following concentrations:
0.005 to 80% by mass of soyasaponin and 0.0005 to 80% by mass of nonionic surfactant; 0.0005 to 50% by mass of soyasaponin and 0.0005 to 50% by mass of nonionic surfactant; 0.0005 of Soyasaponin 0.005 to 5% by mass of non-ionic surfactant; 0.0005 to 0.5% by mass of soyasaponin and 0.0005 to 0.5% by mass of non-ionic surfactant; 0.005% by mass of soyasaponin -80 mass% and nonionic surfactant 0.005 to 80 mass%; soyasaponin 0.005 to 50 mass% and nonionic surfactant 0.005 to 50 mass%; soyasaponin 0.005 to 5 mass% And 0.005 to 5% by mass of a nonionic surfactant; or 0.005 to 0.5% by mass of soyasaponin and 0.005 to 0% by mass of a nonionic surfactant It is 5% by mass,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[33] Preferably, the use according to [32], wherein the composition is a fertilizer, a microbial material, a soil conditioner, a sowing material, or a plant supplement.
[34] The use according to [30] or [31], wherein the composition is a cultivation base material for legumes containing the soyasaponin and a nonionic surfactant at the following concentrations.
0.01 to 100 mass ppm of soyasaponin and 0.01 to 100 ppm by mass of nonionic surfactant; 0.01 to 50 massppm of soyasaponin and 0.01 to 50 ppm by mass of nonionic surfactant; 0.01 of soyasaponin -20 mass ppm and non-ionic surfactant 0.01-20 mass ppm; soyasaponin 0.01-13 mass ppm and non-ionic surfactant 0.01-13 mass ppm; soyasaponin 0.1-10 mass ppm And 0.1 to 10 mass ppm of non-ionic surfactant; 0.1 to 5 mass ppm of soyasaponin and 0.1 to 5 mass ppm of non-ionic surfactant; or 0.1 to 2 mass ppm of soyasaponin and non-ionic 0.1 to 2 mass ppm of the ionic surfactant,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[35] The use according to [34], wherein the cultivation base material is preferably soil, cultivated soil, a medium, a solution for nutrient cultivation or water.
[36] Preferably, the use according to any one of [25] to [35], wherein the growth promotion is an increase in underground weight.

[37] A method for cultivating a legume plant using a soyasaponin and a nonionic surfactant of HLB 9 to 20 as active ingredients.
[38] A method for promoting the growth of legumes, using a soyasaponin and a nonionic surfactant of HLB 9-20 as active ingredients.
[39] The method according to [37] or [38], preferably comprising applying the soyasaponin and the nonionic surfactant to the leguminous plant and then cultivating the same.
[40] The non-ionic surfactant,
Preferably, at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters,
More preferably, at least one selected from the group consisting of polyoxyethylene alkyl ether and polyoxyethylene sorbitan fatty acid ester,
More preferably, polyoxyethylene alkyl ether,
The method according to any one of [37] to [39].
[41] Preferably, the method according to any one of [37] to [40], wherein the nonionic surfactant is HLB 10 to 19.
[42] The soyasaponin is
Preferably, it is soyasapogenol B glycoside,
More preferably, a glycoside of soyasapogenol B, wherein the C-22 position of the soyasapogenol B is a hydroxy group, and a sugar is bonded to the hydroxy group at the C-3 position of the soyasapogenol B Yes,
More preferably, it is soyasaponin Bb,
The method according to any one of [37] to [41].
[43] the legume plant is
Preferably, it is at least one selected from the group consisting of a soybean plant, a kidney bean plant, a chickpea plant, a pea plant, a lentil plant, a legume plant, a fava bean plant and a peanut plant,
More preferably, it is at least one selected from the group consisting of soybeans, kidney beans, chickpeas, peas, lentils, pigeonpeas, fava beans and peanuts.
The method according to any one of [37] to [42].
[44] Preferably, the soyasaponin and the nonionic surfactant are contained in a composition, and the composition is a cultivation base material for a leguminous plant, a pesticide, a fertilizer, a microbial material, a soil conditioner, a seeding agent. The method according to any one of [37] to [43], which is a material or a plant supplement.
[45] Preferably, the composition is a microorganism material, plant essential nutrients, flavonoids, organic acids, amino acids, peptides, nucleosides, nucleotides, nucleobases, sugars, monohydric alcohols, nonionic surfactants, food additives Products, microbial extracts, plant hormones, nod factors, ie lipo-chitooligosaccharides, synthetic lipo-chitooligosaccharides, chitooligosaccharides, chitin compounds, linoleic acid or derivatives thereof, linolenic acid or derivatives thereof, caliquine, acyl-homoserine lactone [44] The method according to [44], further comprising at least one selected from the group consisting of a derivative, a betaine compound, a phenol compound, a catechin, and iron phosphate.
[46] Preferably, the composition according to [44] or [45], wherein the composition contains the soyasaponin and a nonionic surfactant at the following concentrations:
0.005 to 80% by mass of soyasaponin and 0.0005 to 80% by mass of nonionic surfactant; 0.0005 to 50% by mass of soyasaponin and 0.0005 to 50% by mass of nonionic surfactant; 0.0005 of Soyasaponin 0.005 to 5% by mass of non-ionic surfactant; 0.0005 to 0.5% by mass of soyasaponin and 0.0005 to 0.5% by mass of non-ionic surfactant; 0.005% by mass of soyasaponin -80 mass% and nonionic surfactant 0.005 to 80 mass%; soyasaponin 0.005 to 50 mass% and nonionic surfactant 0.005 to 50 mass%; soyasaponin 0.005 to 5 mass% And 0.005 to 5% by mass of a nonionic surfactant; or 0.005 to 0.5% by mass of soyasaponin and 0.005 to 0% by mass of a nonionic surfactant It is 5% by mass,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[47] The method according to [46], wherein the composition is preferably a fertilizer, a microbial material, a soil conditioner, a sowing material, or a plant supplement.
[48] The method according to [44] or [45], wherein the composition is a cultivation base material for legumes containing the soyasaponin and a nonionic surfactant at the following concentrations.
0.01 to 100 mass ppm of soyasaponin and 0.01 to 100 ppm by mass of nonionic surfactant; 0.01 to 50 massppm of soyasaponin and 0.01 to 50 ppm by mass of nonionic surfactant; 0.01 of soyasaponin -20 mass ppm and non-ionic surfactant 0.01-20 mass ppm; soyasaponin 0.01-13 mass ppm and non-ionic surfactant 0.01-13 mass ppm; soyasaponin 0.1-10 mass ppm And 0.1 to 10 mass ppm of non-ionic surfactant; 0.1 to 5 mass ppm of soyasaponin and 0.1 to 5 mass ppm of non-ionic surfactant; or 0.1 to 2 mass ppm of soyasaponin and non-ionic 0.1 to 2 mass ppm of the ionic surfactant,
In each of the concentration ranges, the mass ratio of the nonionic surfactant to the mass of the soyasaponin is preferably 0.001 to 1000 times, more preferably 0.01 to 100 times, more preferably 0.1 to 10 times. Times, more preferably 0.3 to 3 times, more preferably 0.5 to 2 times.
[49] The method according to [48], wherein the cultivation base material is preferably soil, cultivated soil, a medium, a solution for nutrient cultivation or water.
[50] Preferably, the method according to any one of [38] to [49], wherein the growth promotion is an increase in underground weight.

  Hereinafter, the present invention will be described in more detail with reference to Examples, but the present invention is not limited thereto. In the following examples,% means mass% and ppm means mass ppm unless otherwise specified.

Soyasaponin In the following Test Examples 1 to 3, a commercially available soybean saponin preparation (Soybean Saponin 80; Access One, hereinafter referred to as SP80) was used as Soyasaponin. As a result of quantifying soyasaponin in SP80 by LC-MS analysis according to the following procedure, the total content of soyasaponin in SP80 was 57% and the content of soyasaponin Bb was 27%.

<LC-MS analysis conditions>
As an HPLC apparatus and a mass spectrometer, an Agilent 1260 Infinity LC system (Agilent Technology Co., Ltd.) and a TripleTOF4600 system (ABS CYX Co., Ltd.) were used, respectively. The column used was CAPCELL CORE C18 (2.1 × 100 mm, 2.7 μm) (Shiseido Co., Ltd.). As eluents, A: 0.1 v / v% formic acid aqueous solution, B: acetonitrile containing 0.1 v / v% formic acid, gradient conditions: 0 min to 0.1 min / 1 v / v% B → 0.1 min １３13 min / 1 to 99.5 v / v% B, and the flow rate was 0.5 mL / min. The ionization method was ESI (positive mode), and the measurement range was m / z 100-1550.
<Standard product>
Standard products include soyasaponin A1 (NP-000108, AnalytiConDiscovery), soyasaponin A2 (NP-001666, AnalytiCon Discovery), soyasaponin Bb (soyasaponin I # P2505, soyasaponin B Co., Ltd., soyasaponin B) -000100, AnalytiCon Discovery), soyasaponin Ba (soyasaponin V # P2506, Tokiwa Plant Chemical Research Co., Ltd.), and dehydrosoyasaponin I (NP-017040, AnalytiCon Discovery) were used. The standard product was subjected to LC-MS analysis to prepare a calibration curve.
<Quantitative analysis>
SP80 was analyzed by LC-MS to identify soyasaponins A1, A2, Bb, Bc, Ba, and dehydrosoyasaponin I based on HPLC retention time, accurate mass, and MS / MS spectrum. Based on the calibration curve, the content of each soyasaponin in SP80 was quantified. Table 2 shows the quantitative values.

Test 1
As the soybean seed, “Fukuyutaka” (purchased from Nikko Seedling Co., Ltd.) was used. Leonard jar (Soil Science and Plant Nutrition, 1983, 29: 97-100) is filled with about 400 mL of cultivation soil (Takii hydrous cell medium cultivation medium fertilizer type: vermiculite = 1: 1 (volume ratio)), and about 1 cm from the cultivation surface Seeds were sown one by one at a depth of.

YM (Yeast Extract Mannitol) medium (K ₂ HPO ₄ 0.5 g, MgSO ₄ .7H ₂ O 0.2 g, NaCl 0.1 g, Yeast Extract 0.4 g, Mannitol 10 g, distilled water 1 L (pH 6.8)) 1.5% agar (Wako Pure Chemical Industries, Ltd.) was added to prepare an agar medium, and the rhizobium Bradyrhizobium japonicum NBRC14783T strain was grown. The grown rhizobia was inoculated with one platinum loop into 5 mL of YM medium prepared in a 50 mL test tube, and cultured at 30 ° C. for 24 hours at a shaking speed of 250 rpm. 1 mL of the obtained rhizobial culture solution was inoculated into 50 mL of a liquid YM medium of the same composition prepared in a 500 mL volume Sakaguchi flask, and cultured with shaking for about 72 hours. A rhizobial culture solution having a cell turbidity OD600 of about 0.3 was prepared.

  1 mL of the prepared rhizobial culture solution was added dropwise to the seeds sown using a micropipettor. Subsequently, 0.1 mL of a 500 ppm SP80 milliQ aqueous solution (SP80 application section), 0.1 mL of a 500 ppm surfactant suspension (surfactant application section), or 0.1 mL each of the SP80 aqueous solution and the surfactant suspension (Combination application section) was dropped on seeds using a micropipettor. Only the rhizobial culture solution was dropped into the non-application section. Examples of the surfactant include nonionic surfactants such as polyoxyethylene sorbitan monolaurate (“Reodol (registered trademark) TW-L120”; Kao Corporation, HLB = 16.7) or polyoxyethylene alkyl ether (“Emulgen (registered trademark) 707”; Kao Corporation, HLB = 12.1) or a polycarboxylic acid type anionic surfactant (“Demol (registered trademark) P”; Kao Corporation) Using. The SP80 and the surfactant concentration in the cultivated soil were 0.125 ppm, respectively, assuming that they were uniformly diffused in the filled cultivated soil.

The cultivation was performed in an artificial weather device (LPH-411SP, Nippon Medical Chemical Co., Ltd.). Light conditions were set under a fluorescent lamp for a light period of 12 hours (light intensity of 130 μmol / m ² / s) / dark period of 12 hours and a temperature of 25 ° C./20° C. Water was supplied by supplying Milli-Q water appropriately.

  35 days after sowing, the plant from which the soil was washed away was cut into an aboveground part and an underground part, and dried in a dryer (100 ° C., 48 hours), and then the underground dry weight of the plant was measured (n = 4 to 4). 7). The results are shown in Table 3 and FIG. The underground dry weight of the plant increased by about 8% in the SP80-applied section compared to the non-applied section, but decreased in all the surfactant-applied sections. The combination of SP80 and non-ionic surfactant resulted in an underground dry weight of about 22% (SP80 + Rhodol® TW-L120) and about 22% (SP80 + Emulgen® ) 707) Increased. On the other hand, in the combination of SP80 and an anionic surfactant (SP80 + Demol (registered trademark) P), the increase in the underground dry weight was almost the same as that in the SP80 application section (about 10%).

Test 2
As the soybean seed, “Fukuyutaka” (purchased from Nikko Seedling Co., Ltd.) was used. A mixed solution of SP80 MilliQ aqueous solution and HLB 8.1 nonionic surfactant (polyoxyethylene lauryl ether, "Emulgen (registered trademark) 103"; Kao Corporation) (final concentration: 500 ppm of SP80, surface activity) Agent 500 ppm). To 5 g of soybean seeds, 100 mg of rhizobial preparation (“Mamezo” (Tokachi Agricultural Cooperative Association)) and 200 μL of the mixed solution were added, mixed in a 25 mL centrifuge tube, and seed-dressed. To the non-application area, 200 μL of Milli-Q water was added instead of the mixed suspension. After the seed dressing, seeds were sown one by one at a depth of about 1 cm from the surface of the cultivation soil in a Leonard jar filled with cultivation soil (Takiicell cultivation initial fertilization type TM-1, Takii seedling Co., Ltd.) of about 400 mL. The concentrations of SP80 and surfactant in the soil were 0.01 ppm, respectively. Cultivation conditions were the same as in Test 1.

  25 days after sowing, the underground dry weight of the plant was measured (n = 3 to 5). The dry weight of the underground part in the mixed liquid application area was reduced by about 10% compared to the non-application area (Table 4 and FIG. 2).

Test 3
As the soybean seed, “Fukuyutaka” (purchased from Nikko Seedling Co., Ltd.) was used. Leonard jar (Soil Science and Plant Nutrition, 1983, 29: 97-100) is filled with about 400 mL of cultivation soil (Takii hydrous cell medium cultivation medium fertilizer type: vermiculite = 1: 1 (volume ratio)), and about 1 cm from the cultivation surface 3 seeds were sown at a depth of. A rhizobial culture solution was prepared in the same procedure as in Test 1. To the sown seeds, 1 mL of the prepared rhizobial culture solution was dropped using a micropipettor.

  A mixed solution of SP80 milliQ aqueous solution and HLB 8.6 nonionic surfactant (sorbitan monolaurate, "Reodol (registered trademark) SP-L10"; Kao Corporation) (final concentration: SP80 500 ppm, interface Activator (500 ppm). 200 μL of the mixed solution was dropped on the seeds to which the rhizobial culture solution had been dropped, and soybean seeds were cultivated in the same procedure as in Test 1. 200 μL of a 500 ppm SP80 milliQ aqueous solution was dropped into the SP80 application section. Only the rhizobial culture solution was dropped into the non-application section. The concentrations of the soybean saponin preparation and the surfactant in the cultivation soil were each 0.25 ppm. Germinated plants were thinned out to one strain in each jar.

  27 days after sowing, the underground dry weight of the plants was measured (n = 4). The underground dry weight of the SP80 application plot increased by about 10% compared to the non-application plot. The underground dry weight of the mixed solution application section was almost equal to that of the SP80 application section (Table 5 and FIG. 3).

Claims (11)

  A leguminous plant growth promoter comprising soyasaponin and a nonionic surfactant of HLB 9 to 20 as active ingredients.   The nonionic surfactant is at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters The leguminous plant growth promoter according to claim 1, which is:   The leguminous plant growth promoter according to claim 2, wherein the nonionic surfactant is a polyoxyethylene alkyl ether.   The leguminous plant growth promoter according to any one of claims 1 to 3, wherein the soyasaponin is a soyasapogenol B glycoside.   The leguminous plant is at least one selected from the group consisting of soybean plants, common bean plants, chickpea plants, pea genus plants, lentil plants, legumes plants, faba bean plants and peanut plants. The leguminous plant growth promoter according to any one of claims 1 to 4.   The leguminous plant growth promoter according to any one of claims 1 to 5, which is a cultivation base material, a pesticide, a fertilizer, a microbial material, a soil improving agent, a seeding material or a plant supplement for leguminous plants.   A method for promoting the growth of leguminous plants, using soyasaponin and HLB 9 to 20 nonionic surfactants as active ingredients.   The nonionic surfactant is at least one selected from the group consisting of polyoxyethylene alkyl ethers, polyoxyalkylene derivatives, polyoxyethylene sorbitan fatty acid esters, polyoxyethylene sorbitol fatty acid esters, and polyoxyethylene fatty acid esters The method of claim 7, wherein   9. The method according to claim 8, wherein said nonionic surfactant is a polyoxyethylene alkyl ether.   The method according to any one of claims 7 to 9, wherein the soyasaponin is a soyasapogenol B glycoside.   The leguminous plant is at least one selected from the group consisting of soybean plants, common bean plants, chickpea plants, pea genus plants, lentil plants, legumes plants, faba bean plants and peanut plants. A method according to any one of claims 7 to 10.