JP2019151559A - Bifidobacteria proliferation promoter - Google Patents

Abstract

To provide a novel material that can promote the proliferation of bifidobacteria.SOLUTION: A bifidobacteria proliferation promoter contains a wakame seaweed processed product, wakame seaweed-derived protein or wakame seaweed-derived peptide as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a bifidobacteria growth promoter.

  Bifidobacteria are good bacteria that are abundant in the human intestine, and are one of the preferred species in the intestinal flora. Bifidobacteria can normalize the intestinal flora by producing organic acids such as lactic acid, acetic acid, and butyric acid using carbohydrates and dietary fiber in the intestine, and suppressing the growth of bad bacteria. Are known. In addition, it has been reported that it exerts a regulatory function for intestinal immunity and that the number of bifidobacteria decreases due to aging, diet, and stress, so that humans can maintain their health. It is important to maintain bifidobacteria predominately.

  As compounds and materials that promote the growth of bifidobacteria, many have been known, such as fructooligosaccharides, galactooligosaccharides, isomaltoligosaccharides, cyclodextrins, konjac mannans, soy milk, etc., besides these, for example, broccoli, Cauliflower, kale, nazuna, suzushiro, hazazao, tagarashi, taisei, obata natsubana, yamagarashi, sagarashi, takana, karashina, wasabi, yurisawabi, hinano, immediately Bifidobacterium growth promoter (patent document 1), sweet basil, oregano containing 1 or 2 or more types selected from the group consisting of na, turnip, oilseed, cabbage, spinach, komatsuna, celery, parsley, lettuce and apple , Capsicum, bittern, oyster, chrysanthemum, snow white vegetable, Mizuna, arugula, okajiki, scampo, Bifidobacteria growth promoter (Patent Document 2), Bifidobacterium longum, and Bifidobacterium containing one or more selected from the group consisting of bats, tsurmurasaki and cypress A composition for growing bifidobacteria for growing at least one bifidobacteria selected from Bifidobacterium adolescentens, comprising barley stem and / or leaf pulverized material and / or dry powder thereof A bifidobacteria growth composition (Patent Document 3) and the like are known.

  Under such circumstances, further new materials that can promote the growth of bifidobacteria have been demanded.

JP-A-11-266860 JP 2006-333837 A JP 2017-147990 A

  An object of the present invention is to provide a novel material that can promote the growth of bifidobacteria.

  As a result of intensive studies on the above problems, the present inventors have found that a processed product of seaweed and a seaweed-derived protein promote the growth of bifidobacteria, and have made the present invention based on this finding. .

That is, the present invention
(1) A bifidobacterial growth promoter characterized by comprising a seaweed processed product as an active ingredient,
(2) A bifidobacterial growth promoter characterized by comprising a protein derived from wakame as an active ingredient,
(3) A growth promoter for bifidobacteria characterized by comprising a wakame-derived peptide as an active ingredient,
(4) The bifidobacteria growth promoter according to any one of (1) to (3) above, wherein the target bifidobacteria is Bifidobacterium longum.
It is made up of.

  The bifidobacteria growth promoter of the present invention can promote the growth of bifidobacteria.

  The seaweed referred to in the present invention is a seaweed taxonomically belonging to the genus Wakame from the order of the Clevisidae. As a kind of seaweed, there are wakame (Undaria pinnatifida), hirome (U. undarioides), and blue wakame (U. peterseniana), and any of them is preferably used in the present invention. Moreover, there is no restriction | limiting in particular in the part of the seaweed used for this invention, You may use any, such as a leaf and a bud.

  The bifidobacteria growth promoter of the present invention comprises a seaweed processed product, a seaweed-derived protein or a seaweed-derived peptide as an active ingredient.

  The seaweed processed product used in the present invention is a seaweed processed product and is not particularly limited as long as it is ingestible as an edible material. For example, dried seaweed (for example, dried cut seaweed, dried seaweed, etc.), Examples include boiled salted seaweed, salted seaweed, desalted seaweed or seaweed-removed seaweed. Here, desalted seaweed is obtained by desalinating seaweed, dried seaweed, boiled salted seaweed or salted seaweed with a water-containing ethanol solution. Dietary fiber-removed seaweed is obtained by removing dietary fiber from seaweed, dried seaweed, boiled salted seaweed, salted seaweed or desalted seaweed. Hereinafter, the outline of the manufacturing method of a desalinated seaweed and a dietary fiber removal seaweed is shown.

<Method of manufacturing desalted seaweed>
First, the seaweed is washed with hydrous ethanol having a concentration of 10 to 60% (v / v). The washing method is not particularly limited, and examples thereof include a method of immersing seaweed in hydrous ethanol and stirring, a method of treating with ultrasonic waves, and the like. Further, the shape of the seaweed to be washed is not particularly limited, but from the viewpoint of the efficiency of the washing treatment, those that are cut into chips are preferable. Moreover, what is necessary is just to set washing | cleaning time suitably according to the washing | cleaning method, the shape of a seaweed, etc. For example, if it is the method of immersing and stirring the seaweed cut | disconnected in the shape of a chip | tip, it shall be 5-60 minutes Can do. Moreover, what is necessary is just to set the washing | cleaning temperature to the grade which does not affect the outflow of the active ingredient contained in a seaweed, for example, it is preferable that it is 0-30 degreeC. Moreover, after washing | cleaning, you may implement the operation which wash | cleans the wakame filtered again with water-containing ethanol once to 3 times. Further, after washing, the filtered seaweed may be dried and pulverized by a method known per se.

<Method for manufacturing dietary fiber-removed wakame>
First, water is added to the desalted seaweed and stirred, the resulting mixture is centrifuged, the supernatant is discarded, and the precipitate is recovered. Although there is no restriction | limiting in particular in the shape of desalted seaweed, From the viewpoint of the removal efficiency of dietary fiber, what was grind | pulverized to the powder form is preferable. Moreover, stirring conditions can be made into 80-100 degreeC for 5 minutes-10 hours.
Next, the collected precipitate is brought into contact with the alkali metal salt. As the alkali metal salt, for example, an alkali metal salt of an organic acid is preferable, and an alkali metal salt of citric acid (for example, sodium citrate) is more preferable. The amount of the alkali metal salt relative to the precipitate varies depending on the type of the alkali metal salt and the like. For example, when trisodium citrate is used, 100 to 3000 parts by weight, preferably 100 parts by weight of the solid content of the precipitate. Is 200-1500 parts by mass. As the contact method, for example, it is preferable to prepare an aqueous solution of an alkali metal salt, add this to the precipitate, and stir. Moreover, stirring conditions can be made into 2 minutes-4 hours at room temperature (0-30 degreeC)-50 degreeC.
After the contact, the resulting mixture is centrifuged, the supernatant is discarded, and the precipitate is recovered to obtain a dietary fiber removed wakame. The resulting dietary fiber-removed wakame may be dried and pulverized by a method known per se.

  In the process of producing a seaweed processed product (for example, dietary fiber-removed seaweed), a water-soluble extract (hereinafter referred to as “water-soluble seaweed extract”) may be obtained as a by-product. Since this water-soluble wakame extract does not sufficiently exhibit the effect as an active ingredient of the bifidobacteria growth promoter, it is not included in the processed seaweed product used in the present invention.

  The wakame-derived protein used in the present invention (hereinafter also referred to as “wakame protein”) is not particularly limited as long as it is a protein separated from wakame. The outline of the method for producing wakame protein will be described below.

<Method for producing wakame protein>
The seaweed protein can be produced by separating the protein from seaweed or seaweed processed product by a protein separation method known per se. Examples of protein separation methods include (1) softening the tissue by immersing in a pH 3.0 to 5.0 buffer solution, and elution / removal of saccharides, pigments, etc., followed by dissolution using alkalis, A method comprising an acid precipitation step in which an acid is added to adjust the pH to 4.5 to 5.0 and recovered as a precipitate; (2) extraction with an aqueous extractant having a pH higher than the isoelectric point; Isoprecipitation method for precipitating and recovering proteins by acid precipitation treatment that adjusts pH to around the isoelectric point, (3) ion exchanger adsorption method, (4) membrane separation method, (5) alginate lyase, etc. The supernatant is discarded and the precipitate is collected to remove the viscous polysaccharide, and the precipitate is washed with ethanol to remove the pigment, lipid, etc. The method of isolate | separating protein by removing is mentioned. Especially, in this invention, since the method of said (5) can be implemented simply and a wakame protein with favorable flavor is obtained, it is preferable. The seaweed protein separated by the method (5) is insoluble. Insoluble means not soluble in any of water, ethanol and oil.

  In the method for producing seaweed protein, it is preferable to use desalted seaweed as a raw material for seaweed protein, since the extraction rate of protein increases and seaweed protein having a reduced smell unique to seaweed is obtained. Further, in the above method for producing wakame protein, it is preferable to remove water-soluble components such as cellulose by treating with an enzyme such as cellulase because the extraction rate of wakame protein increases. The temperature and pH of the enzyme treatment with alginate lyase, cellulase, etc. may be within the range that is commonly accepted, but the reaction time can be shortened and the enzyme pH can be reduced at the optimum temperature and pH of the enzyme. Desirable for stability.

  Here, the purity of the seaweed protein is determined by the Kjeldahl method (edited by the New Food Analysis Method Editorial Committee, Japan Food Science and Technology Association, “New Food Analysis Method”, published by Kozo, November 30, 1996, p. 33-38. ) To measure the total nitrogen content of the sample and multiply by a coefficient of 6.25 to obtain the protein content.

  The peptide derived from seaweed used in the present invention (hereinafter, also referred to as “wakame peptide”) is not particularly limited as long as it is a peptide obtained by degrading seaweed protein, for example. The outline of the method for producing the seaweed peptide is shown below.

<Method for producing wakame peptide>
A seaweed peptide is manufactured by fractionating from the processed material obtained by processing a seaweed protein with a proteolytic enzyme, for example. Examples of the proteolytic enzyme include protease, trypsin, pancreatin, chymotrypsin, pepsin, papain, etc. Among them, protease is preferably used. The amount of proteolytic enzyme used and the temperature and pH of the treatment with the proteolytic enzyme may be within the conditions that are commonly accepted, but the enzyme is used at the optimal amount, temperature, and pH. This is desirable for shortening the reaction time and stability of the enzyme. There is no particular limitation on the method for separating the peptide from the processed product obtained by the treatment with the proteolytic enzyme. For example, the processed product is centrifuged to remove the undegraded protein, and the supernatant is obtained. A method of obtaining a peptide having a molecular weight of 10,000 or less by treating the supernatant with an ultrafiltration membrane can be mentioned.

  Here, the wakame peptide obtained by the above-described method contains a plurality of specific types of dipeptides as described in JP-A-2003-128694, and at least Val-Tyr, Ile-Tyr and Phe- It contains a hydrophobic amino acid-tyrosine such as Tyr.

  The bifidobacteria growth promoter of the present invention is the above-mentioned processed seaweed product, seaweed protein or seaweed peptide as it is, or a pharmaceutically acceptable additive, food material, food material, and if necessary, a food additive. According to a conventional method, it mixes suitably, For example, it manufactures as a pharmaceutical, a quasi-drug, or food-drinks (a health food, a food for specific health, or a functional indication food is included) aiming at promotion of bifidobacteria growth. Examples of the pharmaceutical or quasi-drug include oral forms such as powders, granules, tablets, microcapsules, soft capsules, hard capsules, syrups, and elixirs. Examples of the food and drink include various food forms such as a solid food, a cream-like or jam-like semi-solid food, a gel-like food, and a beverage. Specific examples include soft drinks, dairy drinks, supplements, cookies, pudding, jelly confectionery, candy, drop, chewing gum, chocolate, yogurt, ice cream, margarine, shortening, mayonnaise or dressing.

  Examples of the additive, food material, food raw material or food additive used in the manufacture of the above-mentioned pharmaceutical, quasi-drug or food and drink include excipients (lactose, maltose, dextrin, corn starch, crystalline cellulose, etc.) Reagents (magnesium stearate, sucrose fatty acid ester, glycerin fatty acid ester, etc.), disintegrants (carboxymethylcellulose calcium, anhydrous calcium hydrogen phosphate, calcium carbonate, etc.), binders (starch paste, hydroxypropyl cellulose, gum arabic) Liquids, etc.), solubilizers (gum arabic, polysorbate 80, etc.), sweeteners (sugar, fructose, glucose liquid sugar, honey, aspartame, etc.), colorants (β-carotene, edible tar dye, riboflavin, etc.), preservatives (Sorbic acid, methyl paraoxybenzoate, sodium sulfite, etc. , Thickeners (sodium alginate, sodium carboxymethylcellulose, sodium polyacrylate, etc.), antioxidants (BHT, BHA, ascorbic acid, tocopherols, etc.), fragrances (mint, strawberry fragrance, etc.), acidulants (citric acid, lactose, etc.) , DL-malic acid, etc.), seasonings (DL-alanine, sodium 5'-inosinate, sodium L-glutamate, etc.), emulsifiers (glycerin fatty acid ester, sucrose fatty acid ester, etc.), pH adjusters (citric acid, citric acid, etc.) Acid trisodium), vitamins, minerals, amino acids and the like.

  When the bifidobacteria growth promoter of the present invention is used as a pharmaceutical, a quasi-drug or a food or drink, in addition to the above-mentioned additive, food material, food raw material or food additive, pharmacologically or food hygienically Other acceptable materials may be appropriately added and mixed by a conventional method. Such a material is not particularly limited, and examples thereof include a coating agent, an absorption accelerator, a stabilizer, a traditional Chinese medicine (herbal medicine extract) and the like.

  The bifidobacteria growth-promoting agent of the present invention has the effect of proliferating various bifidobacteria inhabiting the human intestinal tract, particularly bifidobacteria beneficial to humans, and maintaining and improving the intestinal flora by ingesting orally. Can be expected. In addition, the active ingredient of the bifidobacteria growth promoter of the present invention is derived from wakame, which is widely eaten as a food, and is highly safe when taken orally.

  Further, the bifidobacteria growth promoter of the present invention is added at the time of production of fermented foods and beverages (eg, yogurt, milky beverages, etc.) produced by fermentation of bifidobacteria, so that the growth of bifidobacteria during the production process and It can be used for the purpose of promoting fermentation by bifidobacteria. Furthermore, the bifidobacteria growth promoter of the present invention can be used for the purpose of increasing the culture efficiency of bifidobacteria by adding it to the culture medium for bifidobacterial strain culture.

  The Bifidobacterium species whose growth is promoted by the Bifidobacterium growth promoter of the present invention is not particularly limited. For example, Bifidobacterium longum, Bifidobacterium lactis, Bifido Bifidobacterium infantis, Bifidobacterium adrecentis, Bifidobacterium breve, Bifidobacterium bifidum (Bifidobium) Animalis (Bifidobacterium) M. animalis) and the like, and preferably Bifidobacterium longum.

  The dose of the bifidobacteria growth promoter of the present invention to the living body varies depending on the composition of the bifidobacteria growth promoter, the purpose of administration, the administration route, and the like. For example, when the wakame processed product is orally administered, it is 10 per day for an adult. When the wakame protein is orally administered, the range is 2 to 2000 mg per day for an adult, and when wakame peptide is orally administered, the range is 2 to 2000 mg per day for an adult.

  Hereinafter, the present invention will be specifically described with reference to examples, but the present invention is not limited thereto.

[Manufacture of desalted seaweed]
To 200 g of dried wakame, 4000 mL of 25% (v / v) water-containing ethanol was added, and this was stirred for 60 minutes at a speed “3” using a three-one motor (model: BLh600; manufactured by Shinto Kagaku Co., Ltd.) and washed. After washing, the liquid part was removed by filtration, and 4000 mL of 25% (v / v) hydrous ethanol was added to the solid part, followed by washing under the same conditions. Thereafter, the liquid part was removed by filtration, and the solid part was vacuum-dried and pulverized to obtain about 146.4 g of powdered desalted seaweed. The above operation was repeated twice to obtain about 293 g of powdered desalted seaweed (prototype 1).

[Production of dietary fiber removed wakame]
15000 mL of tap water was added to 50 g of desalted seaweed (prototype 1), stirred at 95 ° C. for 60 minutes, and the resulting mixture was cooled to room temperature. This mixture was centrifuged at 10000 × g and 15 ° C. for 10 minutes, and the supernatant and the precipitate were collected. (The collected supernatant was used in “Production of water-soluble wakame extract” described later.) ). 5000 mL of 2% trisodium citrate aqueous solution was added to the resulting precipitate, and the mixture was stirred at room temperature for 30 minutes, then centrifuged at 10000 × g and 15 ° C. for 10 minutes, the supernatant was discarded, and the precipitate was collected. . The obtained precipitate was freeze-dried and pulverized to obtain 15.5 g of powdered dietary fiber removed wakame (prototype 2).

[Manufacture of wakame protein]
8000 mL of tap water, 160 g of trisodium citrate dihydrate, 1.6 g of anhydrous citric acid, 40 mg of alginate lyase (product name: alginate lyase S; 28000 units / g; manufactured by Amano Enzyme) and desalted seaweed (prototype 1) 201.5g was added and it processed at 45 degreeC for 4 hours, stirring. The treated product was centrifuged at 5000 × g and 15 ° C. for 5 minutes, the supernatant was discarded, and the precipitate was collected.
6000 mL of tap water, 80 g of trisodium citrate dihydrate, 0.8 g of anhydrous citric acid and 40 mg of alginate lyase (product name: alginate lyase S; 28000 units / g; manufactured by Amano Enzyme) were added to the resulting precipitate, Treated for 17 hours at 45 ° C. with stirring. The treated product was centrifuged at 5000 × g and 15 ° C. for 5 minutes, the supernatant was discarded, and the precipitate was collected. After adding 3000 mL of ultrapure water to the obtained precipitate and stirring, the precipitate was washed by centrifuging at 5000 × g and 15 ° C. for 5 minutes and collecting the precipitate four times. After washing, the precipitate was freeze-dried and pulverized to obtain 72.6 g of pulverized material. The operation of adding 700 mL of 100% ethanol to the pulverized product and stirring for 10 minutes, filtering to remove the liquid part, and collecting the solid part was repeated 6 times.
The obtained solid part was vacuum-dried at 40 ° C. for 24 hours to obtain 61.9 g of a dried product. After adding 1500 mL of tap water to 61.0 g of the obtained dried product and adjusting the pH to 4.8 by adding 1N hydrochloric acid thereto, 2 g of cellulase (product name: Cellulase T “Amano” 4; manufactured by Amano Enzyme) was added. The mixture was further stirred at 45 ° C. for 5 hours. The treated product was centrifuged at 5000 × g and 15 ° C. for 5 minutes, the supernatant was discarded, and the precipitate was collected.
After adding 1200 mL of ultrapure water to the resulting precipitate and stirring, the precipitate was washed by centrifuging at 5000 × g and 15 ° C. for 5 minutes and collecting the precipitate three times.
After adding 1000 mL of 50% ethanol to the washed precipitate and stirring, the precipitate was further washed by repeating the operation of centrifuging at 5000 × g and 15 ° C. for 5 minutes and collecting the precipitate four times. After adding 1000 mL of 100% ethanol to the washed precipitate and stirring, the precipitate was further washed by repeating the operation of centrifuging at 5000 × g and 15 ° C. for 5 minutes and collecting the precipitate twice.
After washing, the precipitate was vacuum-dried and pulverized to obtain 50.2 g of powdered seaweed protein (prototype 3; purity of about 80%).

[Production of Wakame Peptide]
After adding 2000 mL of 0.1 M citrate buffer to 100 g of dried wakame and adjusting the pH to 7.0, 100 U of alginate lyase (trade name: alginate lyase S; manufactured by Nagase ChemteX Corporation) was added thereto, and then 45 ° C. For 3 hours. Thereafter, the obtained processed product was centrifuged at 3000 × g for 5 minutes, the supernatant was discarded, and the precipitate was collected to obtain 35 g of a composition containing the protein separated from seaweed.
Next, after adding 1500 g of distilled water to the above composition and homogenizing, 10000 U protease (trade name: Protease S “Amano”; Amano Enzyme) was added to adjust the pH to 8.0. , And treated at 72 ° C. for 18 hours. Thereafter, the mixture was centrifuged at 3000 × g for 5 minutes, and the supernatant was collected. The collected supernatant was ultrafiltered with an ultrafiltration membrane (trade name: FB02-FC-FUS0181; manufactured by Daisen Membrane Systems Co., Ltd.), and the permeate was collected, freeze-dried, pulverized, and powdered. About 10 g of Nowakame Peptide (Prototype 4) was obtained.

[Production of water-soluble wakame extract]
A water-soluble seaweed extract was produced using the supernatant collected in the above-mentioned “production of dietary fiber-removed seaweed”. That is, the supernatant was concentrated with a rotary evaporator, freeze-dried and pulverized to obtain 22.0 g of a powdery water-soluble wakame extract (prototype 5).

[Bifidobacteria growth test]

(1) Culture of Bifidobacteria Bifidobacterium longum subsp. longum JCM1217 strain was inoculated into BL medium (Nissui Pharmaceutical Co., Ltd.) supplemented with 5% horse blood (manufactured by Kojin Bio Inc.), and anaerobic culture (H ₂ : 10%, CO ₂ : at 37 ° C. for 48 hours). 10%, N ₂ : 80%). The obtained cells are appropriately diluted with physiological saline, smeared on each test medium in a plate in an amount containing 2.0 × 10 ³ cfu, and anaerobically cultured at 37 ° C. for 7 days (H ₂ : 10% , CO ₂ : 10%, N ₂ : 80%).
As a test medium, desalted seaweed (prototype 1), dietary fiber-removed seaweed (prototype 2), seaweed protein (prototype 3), seaweed peptide (prototype 4) or MRS agar medium (Oxoid UK) A water-soluble wakame extract (prototype 5) was added in an amount of 0.8% by mass and subjected to heat sterilization at 121 ° C. for 15 minutes. In addition, as a negative control test medium, an MRS agar medium to which none of the prototypes 1 to 5 was added was similarly prepared. Further, a TOS propionic acid agar medium (manufactured by Yakult Yakuhin Kogyo Co., Ltd.) to which none of the prototypes 1 to 5 was added was also prepared as a positive control test medium.
The composition per 1 L of the above MRS agar medium was 10.0 g of peptone, 8.0 g of Laboremco powder, 4.0 g of yeast extract, 20.0 g of glucose, 1.0 mL of sorbitan monooleate, and 2.0 g of dipotassium hydrogen phosphate. Sodium acetate trihydrate 5.0 g, triammonium citrate 2.0 g, magnesium sulfate heptahydrate 0.2 g, manganese sulfate tetrahydrate 0.05 g, manganese sulfate tetrahydrate 0.05 g, agar 10.0 g. The composition per liter of the TOS propionate agar medium is 10.0 g peptone, 1.0 g yeast extract, 3.0 g potassium dihydrogen phosphate, 4.8 g dipotassium monohydrogen phosphate, 3.0 g ammonium sulfate, sulfuric acid Magnesium heptahydrate 0.2 g, L-cysteine hydrochloride monohydrate 0.5 g, sodium propionate 15.0 g, galactooligosaccharide 10 g, and agar 15.0 g.

(2) Evaluation of growth-promoting effect of bifidobacteria For images obtained by photographing the test medium after culture, image analysis software ImageJ (version 1.4.3.67; National Institutes of Health; http: // rsbweb) .Nih.gov / ij), the growth area of Bifidobacteria grown on each test medium was determined. The ratio of the growth area of each test medium when the growth area of the bifidobacteria grown on the TOS propionate agar medium suitable for the growth of bifidobacteria was 100% was defined as the growth promotion rate (%) of the bifidobacteria. . The results are shown in Table 1.

  From the results in Table 1, the growth promoting effect of bifidobacteria was confirmed in the MRS agar medium to which the prototypes 1 to 4 were added. In particular, the samples to which prototypes 2 and 4 were added had a growth promoting effect that exceeded the positive control suitable for the culture of bifidobacteria. On the other hand, in the MRS agar medium to which the prototype 5 was added, the growth promoting effect of bifidobacteria was negligible and was almost the same as the negative control not suitable for the culture of bifidobacteria. Accordingly, it has been found that desalted seaweed, dietary fiber-removed seaweed, seaweed protein and seaweed peptide (particularly dietary fiber-removed seaweed and seaweed peptide) are useful as active ingredients of a bifidobacteria growth promoter.

[Prescription example of Bifidobacterium growth promoter]
Bifidobacterium growth promoter (capsule) containing desalted seaweed (prototype 1), dietary fiber-removed seaweed (prototype 2), seaweed protein (prototype 3) and seaweed peptide (prototype 4) as active ingredients An example of a prescription at the time of preparing an agent and a tablet) is shown.

Formulation Example 1: Capsule containing desalted seaweed Desalted seaweed (prototype 1) 99% by mass
Triglycerin fatty acid ester 1% by mass

Formulation Example 2: Tablet containing dietary fiber-removed seaweed Dietary fiber-removed seaweed (prototype 2) 55% by mass
20% by mass of crystalline cellulose
Reduced maltose 20% by mass
Triglycerin fatty acid ester 5% by mass

Formulation Example 3: Tablets containing wakame protein Wakame protein (prototype 3) 78% by mass
20% by mass of crystalline cellulose
Triglycerin fatty acid ester 2% by mass

Formulation Example 4: Tablet containing wakame peptide Wakame peptide (prototype 4) 82% by mass
Reduced maltose 15% by mass
Triglycerin fatty acid ester 3% by mass

Claims (4)

  A bifidobacteria growth promoter characterized by comprising a seaweed processed product as an active ingredient.   A bifidobacterial growth promoter characterized by comprising a protein derived from wakame as an active ingredient.   A bifidobacteria growth promoter comprising a wakame-derived peptide as an active ingredient.   The bifidobacteria growth promoter according to any one of claims 1 to 3, wherein the target bifidobacteria is Bifidobacterium longum.