JP2018148933A5 - - Google Patents

Info

Publication number

JP2018148933A5

JP2018148933A5 JP2018128583A JP2018128583A JP2018148933A5 JP 2018148933 A5 JP2018148933 A5 JP 2018148933A5 JP 2018128583 A JP2018128583 A JP 2018128583A JP 2018128583 A JP2018128583 A JP 2018128583A JP 2018148933 A5 JP2018148933 A5 JP 2018148933A5

Authority

JP

Japan

Prior art keywords

pluripotent stem

primate pluripotent

suspension

cell

cell assembly

Prior art date

2018-07-05

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
• Global Dossier
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• 241000288906 Primates Species 0.000 claims 18
• 210000004027 cell Anatomy 0.000 claims 13
• 239000000725 suspension Substances 0.000 claims 8
• 210000001778 pluripotent stem cell Anatomy 0.000 claims 7
• 230000001464 adherent effect Effects 0.000 claims 4
• 239000006285 cell suspension Substances 0.000 claims 3
• 230000004069 differentiation Effects 0.000 claims 3
• 210000004039 endoderm cell Anatomy 0.000 claims 3
• 102100023915 Insulin Human genes 0.000 claims 2
• 102100041030 Pancreas/duodenum homeobox protein 1 Human genes 0.000 claims 2
• 101710183548 Pyridoxal 5'-phosphate synthase subunit PdxS Proteins 0.000 claims 2
• 102000043168 TGF-beta family Human genes 0.000 claims 2
• 108091085018 TGF-beta family Proteins 0.000 claims 2
• 238000004113 cell culture Methods 0.000 claims 2
• 239000003102 growth factor Substances 0.000 claims 2
• NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
• 229940088872 Apoptosis inhibitor Drugs 0.000 claims 1
• 101100518002 Danio rerio nkx2.2a gene Proteins 0.000 claims 1
• 102000009024 Epidermal Growth Factor Human genes 0.000 claims 1
• 101800003838 Epidermal growth factor Proteins 0.000 claims 1
• 108050007372 Fibroblast Growth Factor Proteins 0.000 claims 1
• 102000018233 Fibroblast Growth Factor Human genes 0.000 claims 1
• 102100022123 Hepatocyte nuclear factor 1-beta Human genes 0.000 claims 1
• 102100029284 Hepatocyte nuclear factor 3-beta Human genes 0.000 claims 1
• 108700014808 Homeobox Protein Nkx-2.2 Proteins 0.000 claims 1
• 102100027886 Homeobox protein Nkx-2.2 Human genes 0.000 claims 1
• 102100028096 Homeobox protein Nkx-6.2 Human genes 0.000 claims 1
• 101001045758 Homo sapiens Hepatocyte nuclear factor 1-beta Proteins 0.000 claims 1
• 101001062347 Homo sapiens Hepatocyte nuclear factor 3-beta Proteins 0.000 claims 1
• 101000578254 Homo sapiens Homeobox protein Nkx-6.1 Proteins 0.000 claims 1
• 101000578258 Homo sapiens Homeobox protein Nkx-6.2 Proteins 0.000 claims 1
• 101000976075 Homo sapiens Insulin Proteins 0.000 claims 1
• 101100460496 Homo sapiens NKX2-2 gene Proteins 0.000 claims 1
• 101000738523 Homo sapiens Pancreas transcription factor 1 subunit alpha Proteins 0.000 claims 1
• 101000652324 Homo sapiens Transcription factor SOX-17 Proteins 0.000 claims 1
• 101000642523 Homo sapiens Transcription factor SOX-7 Proteins 0.000 claims 1
• 108090001061 Insulin Proteins 0.000 claims 1
• 108090000723 Insulin-Like Growth Factor I Proteins 0.000 claims 1
• 102000004218 Insulin-Like Growth Factor I Human genes 0.000 claims 1
• 102100037878 Pancreas transcription factor 1 subunit alpha Human genes 0.000 claims 1
• 102100030243 Transcription factor SOX-17 Human genes 0.000 claims 1
• 102100036730 Transcription factor SOX-7 Human genes 0.000 claims 1
• 108050003627 Wnt Proteins 0.000 claims 1
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• 238000013019 agitation Methods 0.000 claims 1
• SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 claims 1
• 239000005557 antagonist Substances 0.000 claims 1
• 239000000158 apoptosis inhibitor Substances 0.000 claims 1
• FOIVPCKZDPCJJY-JQIJEIRASA-N arotinoid acid Chemical compound C=1C=C(C(CCC2(C)C)(C)C)C2=CC=1C(/C)=C/C1=CC=C(C(O)=O)C=C1 FOIVPCKZDPCJJY-JQIJEIRASA-N 0.000 claims 1
• 210000003981 ectoderm Anatomy 0.000 claims 1
• 210000001671 embryonic stem cell Anatomy 0.000 claims 1
• 210000001900 endoderm Anatomy 0.000 claims 1
• 230000007613 environmental effect Effects 0.000 claims 1
• 229940116977 epidermal growth factor Drugs 0.000 claims 1
• 229940126864 fibroblast growth factor Drugs 0.000 claims 1
• 239000012530 fluid Substances 0.000 claims 1
• 239000003112 inhibitor Substances 0.000 claims 1
• 229940125396 insulin Drugs 0.000 claims 1
• 210000003716 mesoderm Anatomy 0.000 claims 1
• 238000000034 method Methods 0.000 claims 1
• 235000015097 nutrients Nutrition 0.000 claims 1
• 229930002330 retinoic acid Natural products 0.000 claims 1
• 239000003590 rho kinase inhibitor Substances 0.000 claims 1
• 238000013341 scale-up Methods 0.000 claims 1
• 238000004114 suspension culture Methods 0.000 claims 1
• 229960001727 tretinoin Drugs 0.000 claims 1
• 210000002438 upper gastrointestinal tract Anatomy 0.000 claims 1
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