JP2018126125A - 熱対流型ポリメラーゼ連鎖反応の装置 - Google Patents
熱対流型ポリメラーゼ連鎖反応の装置 Download PDFInfo
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Abstract
【解決手段】 該装置は、第1枠体、第2枠体、温度感知装置、電力供給装置、処理装置及び収容空間を含む。該装置は、従来の加熱金属ブロックの代わりに透明導電フィルムを熱供給部として用いるポリメラーゼ連鎖反応又は逆転写ポリメラーゼ連鎖反応装置であって、試薬容器の底部が透明導電フィルムと接触して昇温させることで熱対流サイクルを構築し、ポリメラーゼ連鎖反応又は逆転写ポリメラーゼ連鎖反応を開始させ、行う装置である。本発明は、特異的なプローブ、蛍光物質、光源及び光信号受信部をさらに含むことで、ポリメラーゼ連鎖反応又は逆転写ポリメラーゼ連鎖反応の産物の定量化を行うことができる。
【選択図】 図5
Description
10 第1枠体
101 第1貫通孔
102 試験管
20 第2枠体
201 第2貫通孔
202 挟持凹溝
30 収容空間
40 フォトダイオード
401 第1フォトダイオード
402 第2フォトダイオード
50 透明導電フィルム
501 上平面
502 下平面
60 接点
70 温度センサ
80 光源
90 光源調整部(光源制御部)
100 処理装置
1010 第3枠体
1020 電力供給部
Claims (17)
- 試薬容器を用いて熱対流により定量のポリメラーゼ連鎖反応又は逆転写ポリメラーゼ連鎖反応を行う装置であって、前記試薬容器に反応物及び試薬が収容され、前記試薬は蛍光物質又はプローブを含み、
前記装置は、
(イ)水平方向に設けられ、且つ第1貫通孔を有する第1枠体と、
(ロ)前記第1枠体の下方に設けられ、且つ前記水平方向に略平行となる第2枠体であって、前記第2枠体は第2貫通孔を有し、前記第2枠体は上表面及び下表面を有し、前記第2枠体の下表面は挟持凹溝を含み、前記挟持凹溝は前記水平方向に略平行となり、前記第2貫通孔は前記挟持凹溝と連通する、第2枠体と、
(ハ)前記挟持凹溝内に設けられた温度感知装置であって、前記温度感知装置はガラス、温度センサ及び接点を含み、前記ガラスは上平面及び下平面を含み、前記上平面又は前記下平面は透明導電フィルムを有し、前記ガラスは前記挟持凹溝と略同一の大きさを有し、且つ前記上平面又は前記下平面で前記挟持凹溝に固定され、前記接点は透明導電フィルムが塗布された側と同一の側に設けられ、前記温度センサは上平面又は下平面に設けられ、前記温度センサは前記透明導電フィルムの温度を測定する、温度感知装置と、
(二)電力供給部及び電力制御部を含む電力供給装置であって、前記電力供給部は前記温度感知装置を加熱するように前記接点に電力を供給し、前記電力制御部は出力される電流を制御する、電力供給装置と、
(ホ)前記蛍光物質又は前記プローブを励起する光源と、
(ヘ)蛍光信号を検出して受信する光信号受信部と、
(ト)システムの処理及び制御を行う処理装置と、
(チ)前記第1枠体と前記第2枠体との間に設けられ、且つ前記試薬容器を設置する収容空間であって、前記試薬容器は、前記第1貫通孔を介して前記収容空間に設置されて、前記第2貫通孔及び前記挟持凹溝を介して前記ガラスの上平面と接触する、収容空間と、を含み、
前記電力供給部は前記接点に電流を供給し、前記ガラスを昇温させ、前記温度センサは前記処理装置にこの状態をフィードバックし、前記処理装置は前記電力制御部に電流を供給させないように通知し、この際に反応が開始し、前記光源の励起により、前記蛍光物質又は前記プローブは特定の波長を有する蛍光を放射し、前記光信号受信部は該蛍光を検出し、前記処理装置に結果をフィードバックする、装置。 - 前記装置はリアルタイム定量ポリメラーゼ連鎖反応及びリアルタイム定量逆転写ポリメラーゼ連鎖反応に適用されるものである、請求項1に記載の装置。
- 前記光信号受信部は、フォトダイオード、光電増倍管、電荷結合素子又は相補型金属酸化物半導体である、請求項1に記載の装置。
- 前記光源は、LED又はレーザランプである、請求項1に記載の装置。
- 前記光信号受信部は前記光源と略垂直な角度をなす、請求項1に記載の装置。
- 前記光源のオン/オフを制御する光源制御部をさらに含む、請求項1に記載の装置。
- 前記光源が前記第2貫通孔の直下に位置する場合は、前記光信号受信部は、前記収容空間に位置し、且つ前記光源に略垂直となる、請求項5に記載の装置。
- 前記光源が前記収容空間に位置する場合は、前記光信号受信部は、前記第2貫通孔の下方に位置し、且つ前記光源に略垂直となる、請求項5に記載の装置。
- 前記ガラスに塗布された透明導電フィルムの材料は、酸化スズ、酸化インジウム、酸化亜鉛又は酸化インジウムスズである、請求項1に記載の装置。
- 前記ガラスの他方側には、非特異的な光をフィルタリングする短波長透過フィルタリング物質が塗布されている、請求項9に記載の装置。
- 試薬容器は略垂直な角度で前記第1貫通孔を介して前記収容空間に設置されて、前記第2貫通孔及び前記挟持凹溝を介して前記ガラスの上平面と接触する、請求項1に記載の装置。
- 前記収容空間の数が前記試薬容器の数と同一であり、且つ各前記収容空間の高さが前記試薬容器内に収容された試薬の高さと略同一であり、前記収容空間は、試薬容器においてポリメラーゼ連鎖反応を行う際に、外部温度の干渉を遮断する、請求項1に記載の装置。
- 試薬容器を用いて熱対流によりポリメラーゼ連鎖反応を行う装置であって、前記試薬容器に反応物及び試薬が収容され、
前記装置は、
(イ)水平方向に設けられ、且つ第1貫通孔を有する第1枠体と、
(ロ)前記第1枠体の下方に設けられ、且つ前記水平方向に略平行となる第2枠体であって、前記第2枠体は第2貫通孔を有し、前記第2枠体は上表面及び下表面を有し、前記第2枠体の下表面は挟持凹溝を含み、前記挟持凹溝は前記水平方向に略平行となり、前記第2貫通孔は前記挟持凹溝と連通する、第2枠体と、
(ハ)前記挟持凹溝内に設けられた温度感知装置であって、前記温度感知装置はガラス、温度センサ及び接点を含み、前記ガラスは上平面及び下平面を含み、前記上平面又は前記下平面は透明導電フィルムを有し、前記ガラスは前記挟持凹溝と略同一の大きさを有し、且つ前記上平面又は前記下平面で前記挟持凹溝に固定され、前記接点は透明導電フィルムが塗布された側と同一の側に設けられ、前記温度センサは上平面又は下平面に設けられ、前記温度センサは前記透明導電フィルムの温度を測定する、温度感知装置と、
(二)電力供給部及び電力制御部を含む電力供給装置であって、前記電力供給部は前記温度感知装置を加熱するように前記接点に電力を供給し、前記電力制御部は出力される電流を制御する、電力供給装置と、
(ホ)システムの処理及び制御を行う処理装置と、
(ヘ)前記第1枠体と前記第2枠体との間に設けられ、且つ前記試薬容器を設置する収容空間であって、前記試薬容器は、前記第1貫通孔を介して前記収容空間に設置されて、前記第2貫通孔及び前記挟持凹溝を介して前記ガラスの上平面と接触する、収容空間と、を含み、
前記電力供給部は前記接点に電流を供給し、前記ガラスを昇温させ、前記温度センサは前記処理装置にこの状態をフィードバックし、前記処理装置は前記電力制御部に電流を供給させないように通知し、この際に反応が開始する、装置。 - 前記装置はポリメラーゼ連鎖反応及び逆転写ポリメラーゼ連鎖反応に適用されるものである、請求項13に記載の装置。
- 前記透明導電フィルムの材料は、酸化スズ、酸化インジウム、酸化亜鉛又は酸化インジウムスズである、請求項13に記載の装置。
- 試薬容器は略垂直な角度で前記第1貫通孔を介して前記収容空間に設置されて、前記第2貫通孔及び前記挟持凹溝を介して前記ガラスの上平面と接触する、請求項13に記載の装置。
- 前記収容空間の数が前記試薬容器の数と同一であり、且つ各前記収容空間の高さが前記試薬容器内に収容された試薬の高さと略同一であり、前記収容空間は、試薬容器においてポリメラーゼ連鎖反応を行う際に、外部温度の干渉を遮断する、請求項13に記載の装置。
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