JP2018080116A - Fucoxanthin-containing composition - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a fucoxanthin-containing composition in which the heat stability of fucoxanthin is improved.SOLUTION: A fucoxanthin-containing composition contains fucoxanthin or a derivative thereof, and Euterpe oleracea pulp or Euterpe oleracea extract.SELECTED DRAWING: None

Description

  The present invention relates to a fucoxanthin-containing composition with improved thermal stability of fucoxanthin.

  Fucoxanthin is a carotenoid pigment having physiological activity such as anti-obesity action and anti-diabetic action. Fucoxanthin is easily decomposed by heat and light, etc., so when using it in health foods, confectionery, beverages, etc., the content of fucoxanthin decreases during the flow process during heat sterilization. was there.

  Until now, the technology which stabilizes fucoxanthin by mix | blending tocopherol, ascorbic acid, etc. with respect to fucoxanthin, and disperse | distributing it to vegetable oil exists (patent document 1). However, the above technique has a problem that it is difficult to use for soft drinks, confectionery and the like because it is dispersed in oil, and it cannot be said that the above problem has been completely solved.

International Publication WO2011 / 058773

  An object of the present invention is to provide a fucoxanthin-containing composition with improved thermal stability of fucoxanthin.

  As a result of intensive studies to solve the above problems, the present inventors have found that the thermal stability of fucoxanthin can be improved by adding acai pulp or acai extract to fucoxanthin. The invention has been completed.

That is, according to the present invention, the following inventions are provided.
(1) A fucoxanthin-containing composition comprising fucoxanthin or a derivative thereof and acai pulp or acai extract.
(2) The fucoxanthin-containing composition according to (1), wherein the fucoxanthin is derived from microalgae.
(3) The fucoxanthin-containing composition according to (1) or (2), wherein the fucoxanthin is water-solubilized fucoxanthin.
(4) The fucoxanthin-containing composition according to any one of (1) to (3), which is an aqueous solution or a dry solid.

   According to the present invention, the thermal stability of fucoxanthin can be improved by blending acai fruit pulverized pulp or extract containing an antioxidant component. In addition, oil can only be used for blending into soft capsules and the like, but by using the present invention, it becomes possible to blend fucoxanthin into health foods, confectionery, beverages, etc. in a stabilized state. .

FIG. 1 shows the results of a thermal stability test of a water-solubilized fucoxanthin solution using various fruit pulps. FIG. 2 shows the result of the thermal stability test of the water-solubilized fucoxanthin solution at 40 ° C. FIG. 3 shows the results of a thermal stability test of a water-solubilized fucoxanthin solution at 60 ° C. FIG. 4 shows the results of a thermal stability test of a water-solubilized fucoxanthin solution at 90 ° C.

Hereinafter, the present invention will be described more specifically.
The fucoxanthin-containing composition of the present invention contains fucoxanthin or a derivative thereof and acai pulp or acai extract.

(1) About Fucoxanthin Fucoxanthin is acetic acid [(1S, 3R) -3-hydroxy-4-[(3E, 5E, 7E, 9E, 11E, 13E, 15E) -18-[(1S, 4S, 6R ) -4-Hydroxy-2,2,6-trimethyl-7-oxabicyclo [4.1.0] heptan-1-yl] -3,7,12,16-tetramethyl c-17-oxooctadeca- 1,3,5,7,9,11,13,15-octenyldene] -3,5,5-trimethylcyclohexyl] ester. In the present invention, a derivative of fucoxanthin may be used. Examples of fucoxanthin derivatives include fucoxanthinol, amarothiaxanthine, 19′-hexanoyloxyfucoxanthin, 19′-hexanoyloxy-4-ketofucoxanthin and the like.

  Fucoxanthin is derived from natural products (for example, those derived from brown algae such as kelp, hijiki or wakame, or those derived from microalgae), chemically synthesized fucoxanthin, fucoxanthin produced by gene recombination Any of these may be used. Two or more fucoxanthins having different production methods may be used in combination. Among these, it is preferable to use fucoxanthin derived from microalgae.

  Fucoxanthin derived from microalgae can be obtained by extracting fucoxanthin from microalgae. Extraction can be performed, for example, by adding ethanol or an ethanol solution to microalgae to extract fucoxanthin. Since the fucoxanthin extract obtained as described above contains impurities such as carotenoids other than fucoxanthin, lipids and polysaccharides, liquid-liquid distribution using an appropriate solvent, extraction such as supercritical fluid extraction, Fucoxanthin may be purified by crystallization, precipitation, chromatography, or a combination thereof.

  In the present invention, examples of the microalgae include, but are not particularly limited to, diatoms, haptophytes, pingiosae, golden algae.

The microalgae belonging to the diatom class include the genus Chaetoceros, Phaeodactylum, Cyclotella, Skeletonema, Odontella, or Nitella. Although microalgae is mentioned, it is not specifically limited.
Examples of the microalgae belonging to the haptophyceae class include, but are not particularly limited to, microalgae belonging to the genus Isochrysis or Pavlova.

  Examples of the microalgae belonging to the class Pinguiophyta include, but are not particularly limited to, microalgae belonging to the genus Pinuiochrysis, Phaeomonas, or Polypodochrysis.

  Examples of the microalgae belonging to the golden alga class include, but are not limited to, microalgae belonging to the genus Ochromonas, Dinobryon, Poteriochromonas, or Epipyxis. .

  The method for extracting fucoxanthin used in the present invention from microalgae is not particularly limited. The state of the microalgae as the raw material may be wet or dry. When using the dried raw material, it is preferable to crush.

The medium for culturing the microalgae is not particularly limited, and for example, artificial seawater or a seawater to which nutrient salts such as nitrogen and phosphoric acid, vitamins, and trace elements are added may be used.
Culturing can be performed under light irradiation conditions. Irradiation may be continuous irradiation or photoperiodic irradiation. Extraction of fucoxanthin from the cultured microalgae can be performed by a conventional method.

  Although the solvent for extracting fucoxanthin from microalgae is not particularly limited, an organic solvent can be used, for example, alcohols such as methanol, ethanol, propanol, isopropanol, n-butanol, and ketones such as methyl ethyl ketone and acetone. , Esters such as methyl acetate and ethyl acetate, organochlorine hydrocarbons such as chloroform, aliphatic hydrocarbons such as hexane, aromatic hydrocarbons such as benzene and toluene, and the like can be used. Two or more solvents can be used in combination.

  The solvent can be used in a ratio of 1: 100 to 100: 1, preferably 1:50 to 50: 1, with respect to the mass of the dried microalgae. Extraction using a solvent can be performed by a conventional method. For example, when ethanol is used as a solvent, the temperature is 0 ° C. to 60 ° C., preferably 5 ° C. to 40 ° C., for 0.5 hour to 100 hours. Preferably, extraction can be performed for 12 hours to 72 hours. In extraction, if necessary, stirring may be performed with ultrasonic waves, a stirrer, or the like. Fucoxanthin can be separated and purified by using the obtained solvent extract as it is or after removing the residue, and using ion exchange chromatography or reverse phase chromatography using a liquid high performance chromatograph.

  Powdery fucoxanthin can be obtained by removing the solvent from the extract or purified solution obtained above. The method for removing the solvent is not particularly limited, and a known method such as distillation under reduced pressure can be used.

  The fucoxanthin in the present invention may be water-solubilized fucoxanthin. Examples of the water-solubilized fucoxanthin include fucoxanthin clathrated with γ-cyclodextrin, but are not particularly limited.

  The fucoxanthin content in the composition of the present invention is not particularly limited, but is generally 0.0001 mg / mL to 1 mg / mL, preferably 0.001 mg / mL to 0.1 mg / mL.

(2) About Acai Pulp or Acai Extract Acai (Japanese name: Wakaba cabbage palm, scientific name: Euterpe oleracea) is a palm family plant native to Brazil. The fruit looks like blueberries and is edible. The site of acai used in the present invention is not particularly limited as long as it contains acai fruit.

  Acai pulp is fruit juice containing water-insoluble solid components obtained from acai fruit. Acai pulp is obtained by squeezing the fruit of acai, but if necessary, the husks and seeds may be removed from the fruit before squeezing. The seed may be removed by filtration or the like.

  Acai extract is acai pulp or acai clear fruit juice (clear fruit juice obtained by clarifying acai pulp by microfiltration, enzyme treatment, ultrafiltration, etc.) with water and / or organic solvent. Refers to the extract. Although there is no particular limitation on the extraction method, the extraction can be carried out by stirring or column extraction using water or hot water, or a mixed solvent of water and an organic solvent. As the organic solvent, alcohols, ethers, esters and the like can be used, but water-soluble organic solvents such as ethanol, methanol, acetone and the like are preferable. Two or more organic solvents may be mixed and used. The amount of the solvent used is not particularly limited. For example, the amount of the solvent is not less than 10 times, preferably not less than 20 times, preferably not more than 100 times, with respect to the above-mentioned acai pulp or acai transparent fruit juice (dry weight). . The extraction temperature and extraction time can be appropriately set according to the type of solvent, and are, for example, 10 to 100 ° C. and 30 minutes to 24 hours.

  The extract may be used as an extracted solution, but if necessary, concentration (concentration by organic solvent, vacuum concentration, membrane concentration, etc.), dilution, purification (activated carbon treatment, resin adsorption treatment, ion exchange resin treatment) , Membrane separation, etc.), sterilization and other treatments may be performed. Further, the extracted solution may be subjected to a treatment such as concentration to dryness, spray drying, freeze drying, etc., and used as a dried product.

  The content of acai pulp or acai extract in the composition of the present invention is not particularly limited, but is generally 0.1% by mass to 99% by mass, and preferably 1% by mass to 95% by mass.

(3) About fucoxanthin-containing composition The form of the fucoxanthin-containing composition of the present invention is not particularly limited and may be liquid or solid (for example, dry solid such as dry powder), but preferably liquid (water In a state dissolved or dispersed / emulsified in a liquid, in a state dissolved in an organic solvent, or in the form of an oil), and more preferably an aqueous solution.

  The fucoxanthin-containing composition of the present invention can be used for various applications such as foods and drinks, cosmetics, pharmaceuticals, feeds, and research reagents.

  Examples of food include breads such as bread, butter roll and bagels; pastries such as baked goods, short breads and cakes; noodles such as udon and soba noodles; cooked rice such as rice, rice balls and rice cakes; Rice cakes such as rice cakes, kinako rice cakes and grated rice cakes; Pasta foods such as spaghetti, fettuccine, penne, erique, ravioli and lasagna; And food for the sick or elderly such as mousse, jelly and soup.

  Examples of beverage applications include juices such as vegetable juice, orange juice and apple juice; milk beverages such as milk and fermented milk; alcoholic beverages such as fruit and sake; tea beverages such as green tea, tea and herbal tea be able to.

  Beverages are various additives acceptable for beverages, such as sugar adjusters (sugars such as sucrose, fructose, glucose, lactose, maltose, fructose glucose liquid sugar, oligosaccharides; sugar alcohols such as erythritol, maltitol, xylitol; Indigestible dextrin, dietary fiber such as agar), high intensity sweetener (sucralose, stevia, acesulfame potassium, saccharin sodium, aspartame, glycyrrhizin, dipotassium glycyrrhizinate, thaumatin, etc.), emulsifier (sucrose fatty acid ester, lecithin, etc.), Stabilizers (soy polysaccharides, pectin, carrageenan, gum arabic, gellan gum, xanthan gum, guar gum, carboxymethylcellulose (CMC), etc., antioxidants (tocopherol, cysteine hydrochloride, etc.), milk (raw milk, skimmed milk powder, cream, condensed milk) Etc.), pigment Anthocyanin-based dye, carotenoid-based pigments, safflower yellow pigment, gardenia, caramel, edible tar dyes, etc.), perfumes, preservatives, preservatives, may contain such as antifungal agents.

  Moreover, the utilization as food for specified health, a nutritional supplement, a nutrient, what is called a health food is also mentioned.

  Cosmetics include milk, cream, lotion, beauty liquid, pack, cleanser, foundation, blusher, lipstick and other makeup cosmetics, hair nourishing, hair tonic, shampoo, rinse and other scalp cosmetics, dispersions, ointments, Uses for liquids, aerosols and patches are listed.

  Examples of pharmaceuticals include anti-inflammatory agents, angiogenesis inhibitors, virus-related malignant tumor therapeutic agents, and antioxidant / cell activity activators. Neutral fat absorption regulator, anti-obesity active agent, lipid metabolic disease treatment / prevention, anti-tumor agent, cancer cell growth inhibitor, blood sugar level inhibitor, scalp external medicine, anti-diabetic agent, hyperlipidemia prevention / Therapeutic agents, external preparations for skin, nerve cell protecting agents, animal drugs and the like can be mentioned. Examples of pharmaceutical forms include tablets, powders, capsules and the like, but are not particularly limited.

  Examples of the feed include, but are not particularly limited to, pet feed, livestock feed, and fishery feed. Examples of the feed for pets include feeds for mammals such as dogs, cats, rabbits, hamsters and squirrels; and feeds for birds such as budgerigars, pigeons, bunchos and juvenile pine. Examples of livestock feed include feed for mammals such as cattle, pigs, sheep and goats; and feed for birds such as chickens, chabos, quails, turkeys, ducks, geese and ostriches. Furthermore, examples of the feed for seafood include feed for cultured fish such as red sea bream, yellowtail, eel and shrimp; feed for ornamental fish such as carp, goldfish, discus, arowana and neon tetra.

  The present invention will be specifically described by the following examples, but the present invention is not limited to the examples.

Example 1: Thermal stability test 1 of water-solubilized fucoxanthin solution
A test solution was prepared by adding water to various fruit pulps (manufactured by Flutter Flutter) shown in FIG. 1 and diluting 5 times and removing fibers by centrifugation. Acai 5-fold concentrated extract (manufactured by Flutta Flutta) was diluted with water twice and used as a test solution. As a control, a 10% sucrose solution adjusted to pH 4.0 was used as a test solution, and water-solubilized fucoxanthin powder (manufactured by Phytolock) was added to each test solution at 2 mg / mL (pure fucoxanthin concentration 0.02 mg / mL). Dissolved to a concentration. Dispense 1 mL of these solutions into brown vials, set them in a heat block set at 60 ° C, heat-treat for 0, 1, 5, 10, 20, 40, 60, 120 minutes, cool in the refrigerator, and then with ethanol Diluted 2-fold. At this time, gelatinous substances such as polysaccharides were produced by addition of ethanol, and after removal by centrifugation, the fucoxanthin concentration was measured by high performance liquid chromatography to compare the effect on fucoxanthin residual rate.

  The measurement results are shown in FIG. As shown in FIG. 1, the acai pulp and the acai 5-fold concentrated extract showed the effect of increasing the thermal stability of the water-solubilized fucoxanthin.

Example 2: Thermal stability test 2 of water-solubilized fucoxanthin solution
In the same manner as in the sample preparation method of Example 1 above, Acai pulp (manufactured by Flutter Flutter) was diluted 5 times with water, and the fiber was removed by centrifugation. Acai 5 times concentrated extract (manufactured by Flutter Flutter) Then, a fucoxanthin-containing sample solution of a sucrose solution was prepared and heat-treated at a temperature of 40 ° C., 60 ° C., and 90 ° C. with each n = 3, and subjected to high performance liquid chromatography to examine fucoxanthin residual rate.

  The measurement results are shown in FIGS. As shown in FIGS. 2 to 4, the acai pulp and the acai 5-fold concentrated extract showed the effect of increasing the thermal stability of the water-solubilized fucoxanthin.

Claims (4)

A fucoxanthin-containing composition comprising fucoxanthin or a derivative thereof and acai pulp or acai extract. The fucoxanthin-containing composition according to claim 1, wherein the fucoxanthin is fucoxanthin derived from microalgae. The fucoxanthin-containing composition according to claim 1 or 2, wherein the fucoxanthin is water-solubilized fucoxanthin. The fucoxanthin-containing composition according to any one of claims 1 to 3, which is an aqueous solution or a dry solid.