JP2017527281A - 物体の分画のための装置および分画方法 - Google Patents
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Abstract
Description
−分画チャンネルの同じ体積(標準形状で4cm/流体レールで4cm);
−分離のための同じ流体流量(流体ガイドの流量を除いて、分離流れとして0.8ml/min);
−注入された同じサンプル。
a)移動相に分散される細胞材料サンプルの調製;
b)生理食塩水および生体適合性溶液(移動相)連続流れによって、ほぼ1つの毛細管チャンネルを備える適切な分画装置の横断面に垂直な方向への、サンプルの注入;
c)装置へのサンプルの溶出;
d)開始サンプルから出発して集団または亜集団において単離された異なる細胞種によって構成される分画収集。
Claims (11)
- 分散液体中の分散相の動的分画のための装置であって:
分画チャンネル(221、212、222)と、
そこを介して第1の閉込め流体が分画チャンネルに注入可能な第1の注入ポート(321)と、そこを介して第2の閉込め流体が分画チャンネル(221、212、222)に注入可能な第2の注入ポート(321)と、
第1および第2の注入ポート(321)の間に設けられた第3の注入ポート(311)であって、そこを介して分散相を移送するための溶出流体が分画チャンネル(221、212、222)に注入可能な第3の注入ポート(311)と、を備え、
分画チャンネル(221、212、222)の第1の端部は、それぞれ第1から第3の注入ポート(311、321)に対応する第1から第3の末端部(241、251)を含み、
第1から第3の末端部(241、251)は、第1および第2の閉込め流体がそれぞれ第1および第2の所定流量を有すると共に溶出流体が第3の所定流量を有するような寸法であり、第1および第2の閉込め流体の間に溶出流体を閉じ込めるために、第3の所定流量が第1および第2の流量より大きい、装置において:
第1および第2の注入ポート(321)にそれぞれ対応する第1および第2の末端部(251)の基部の幅は、第3の注入ポート(311)に対応する第3の末端部(241)の基部の幅の25%から50%の範囲内である、ことを特徴とする装置。 - 第1から第3の末端部(241、251)は、それぞれの長手軸が互いにかつ分画チャンネル(221、212、222)の長手軸に平行であり、溶出流体が第1および第2の閉込め流体に平行に流れるよう、に構成されていることを特徴とする請求項1に記載の装置。
- 第1から第3の末端部(241、251)は、アーチ形状であることを特徴とする請求項1または2に記載の装置。
- 分画チャンネル(221、212、222)に分散流体中の分散相を注入するためのサンプル注入ポート(331)をさらに含み、サンプル注入ポート(331)が、分画チャンネル(221、212、222)内の溶出流体中への分散相の注入を可能とするように配置されていることを特徴とする請求項1〜3のいずれか1項に記載の装置。
- 第1から第3の注入ポート(311、321)と流体連通し、第3の所定の流量で溶出流体の流れを制御するように適合された、少なくとも1つの第1の流れ制御手段(441)をさらに備え、
少なくとも1つの第1の流れ制御手段(441)が、第1の所定の流量で第1の閉込め流体の流れを制御し、第2の所定の流量で第2の閉込め流体の流れを制御するように、さらに構成されることを特徴とする請求項1〜4のいずれか1項に記載の装置。 - 分散流体中の分散相の動的分画のための方法であって:
第1の注入ポート(321)を介する第1の閉込め流体および第2の注入ポート(321)を介する第2の閉込め流体を、分画チャンネル(221、212、222)に注入するステップであって、第1および第2の閉込め流体がそれぞれ第1および第2の所定の流量で供給されるステップと、
第1および第2の注入ポート(321)の間に設けられた第3の注入ポート(311)を介して、移動相を供給するための溶出流体を分画チャンネル(221、212、222)に注入するステップであって、溶出流体が第3の所定の流量で供給されるステップと、を備え、
第1および第2の閉込め流体の間に溶出流体を閉じ込めるために、第3の所定流量が第1および第2の所定流量より大きい、方法において:
第1および第2の閉込め流体の第1および第2の所定流量が、溶出流体の第3の流量の5%から25%の範囲内であることを特徴とする方法。 - 第1および第2の閉込め流体および溶出流体が、それぞれ、分画チャンネル(221、212、222)の第1の端部の第1から第3の末端部(241、251)に対応して、分画チャンネル(221、212、222)内に注入されることを特徴とする請求項6に記載の方法。
- 第1および第2の閉込め流体の第1および第2の所定流量が、第3の所定流量の10%であることを特徴とする請求項6または7に記載の方法。
- 分散流体中の分散相のサンプルを調製するステップと、
分画チャンネル(221、212、222)を介して流れる溶出流体中に、サンプル注入ポート(331)を介して、調製したサンプルを導入するステップと、
分画チャンネル(221、212、222)から調整したサンプルを溶出するステップと、
調整されたサンプルを導入するステップの後であって調整したサンプルを溶出するステップの前に、溶出流体の流れを中断するステップと、
をさらに備えることを特徴とする請求項6〜8のいずれか1項に記載の方法。 - 分画条件および/または注入されたサンプルの種類に基づいて、溶出流体および/または第1および第2の閉込め流体の流量を設定するステップ、をさらに備えることを特徴とする請求項6〜9のいずれか1項に記載の方法。
- 分散相は、生物学的物質、好ましくは細胞および/または幹細胞を含むことを特徴とする請求項6〜10のいずれか1項に記載の方法。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
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ITVI2014A000214 | 2014-08-20 | ||
ITVI20140214 | 2014-08-20 | ||
PCT/IB2015/056195 WO2016027204A1 (en) | 2014-08-20 | 2015-08-14 | Device for the fractionation of objects and fractionation method |
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JP2017527281A true JP2017527281A (ja) | 2017-09-21 |
JP6487035B2 JP6487035B2 (ja) | 2019-03-20 |
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US (1) | US10788463B2 (ja) |
EP (2) | EP4155725A1 (ja) |
JP (1) | JP6487035B2 (ja) |
KR (1) | KR101984559B1 (ja) |
CN (1) | CN107076708B (ja) |
CA (1) | CA2958118C (ja) |
DK (1) | DK3183568T3 (ja) |
ES (1) | ES2936343T3 (ja) |
FI (1) | FI3183568T3 (ja) |
HR (1) | HRP20230049T1 (ja) |
HU (1) | HUE060950T2 (ja) |
LT (1) | LT3183568T (ja) |
PL (1) | PL3183568T3 (ja) |
PT (1) | PT3183568T (ja) |
RS (1) | RS63882B1 (ja) |
SI (1) | SI3183568T1 (ja) |
WO (1) | WO2016027204A1 (ja) |
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WO2019198178A1 (ja) * | 2018-04-11 | 2019-10-17 | 株式会社島津製作所 | フィールドフローフラクショネーション装置 |
US11971392B2 (en) * | 2019-06-25 | 2024-04-30 | Wyatt Technology, Llc | Sealing structure for a field flow fractionator |
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Publication number | Publication date |
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US10788463B2 (en) | 2020-09-29 |
KR20170039742A (ko) | 2017-04-11 |
EP3183568A1 (en) | 2017-06-28 |
EP4155725A1 (en) | 2023-03-29 |
PT3183568T (pt) | 2023-01-16 |
KR101984559B1 (ko) | 2019-05-31 |
CA2958118C (en) | 2019-05-21 |
EP3183568B1 (en) | 2022-10-26 |
US20170241959A1 (en) | 2017-08-24 |
JP6487035B2 (ja) | 2019-03-20 |
LT3183568T (lt) | 2023-01-25 |
HRP20230049T1 (hr) | 2023-03-03 |
FI3183568T3 (fi) | 2023-01-31 |
PL3183568T3 (pl) | 2023-03-27 |
CN107076708B (zh) | 2020-02-28 |
CN107076708A (zh) | 2017-08-18 |
ES2936343T3 (es) | 2023-03-16 |
WO2016027204A1 (en) | 2016-02-25 |
CA2958118A1 (en) | 2016-02-25 |
HUE060950T2 (hu) | 2023-04-28 |
SI3183568T1 (sl) | 2023-02-28 |
DK3183568T3 (da) | 2023-01-30 |
RS63882B1 (sr) | 2023-02-28 |
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