JP2017513525A - 液滴タグ化のためのシステムおよび方法 - Google Patents

液滴タグ化のためのシステムおよび方法 Download PDF

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JP2017513525A
JP2017513525A JP2017506636A JP2017506636A JP2017513525A JP 2017513525 A JP2017513525 A JP 2017513525A JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017506636 A JP2017506636 A JP 2017506636A JP 2017513525 A JP2017513525 A JP 2017513525A
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droplet
nucleic acid
droplets
cells
molecular species
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Japanese (ja)
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JP2017513525A5 (OSRAM
Inventor
ブラッドリー・イー・バーンスタイン
ロバート・ニコル
デイビッド・エイ・ウェイツ
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General Hospital Corp
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General Hospital Corp
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00583—Features relative to the processes being carried out
    • B01J2219/00599—Solution-phase processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/00722—Nucleotides
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00718—Type of compounds synthesised
    • B01J2219/0072—Organic compounds
    • B01J2219/0074—Biological products
    • B01J2219/00743—Cells
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/08—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support
    • C40B50/10—Liquid phase synthesis, i.e. wherein all library building blocks are in liquid phase or in solution during library creation; Particular methods of cleavage from the liquid support involving encoding steps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1028—Sorting particles

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  • Life Sciences & Earth Sciences (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
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  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Dispersion Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2017506636A 2014-04-17 2015-04-17 液滴タグ化のためのシステムおよび方法 Pending JP2017513525A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461981123P 2014-04-17 2014-04-17
US61/981,123 2014-04-17
PCT/US2015/026338 WO2015161177A1 (en) 2014-04-17 2015-04-17 Systems and methods for droplet tagging

Publications (2)

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JP2017513525A true JP2017513525A (ja) 2017-06-01
JP2017513525A5 JP2017513525A5 (OSRAM) 2018-05-31

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JP2017506636A Pending JP2017513525A (ja) 2014-04-17 2015-04-17 液滴タグ化のためのシステムおよび方法

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US (1) US20170028377A1 (OSRAM)
EP (1) EP3132072B1 (OSRAM)
JP (1) JP2017513525A (OSRAM)
CN (1) CN106661763A (OSRAM)
AU (1) AU2015247416A1 (OSRAM)
CA (1) CA2945794A1 (OSRAM)
WO (1) WO2015161177A1 (OSRAM)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LT3305918T (lt) 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
US20150298091A1 (en) 2014-04-21 2015-10-22 President And Fellows Of Harvard College Systems and methods for barcoding nucleic acids
CN107614096A (zh) 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
WO2016168584A1 (en) 2015-04-17 2016-10-20 President And Fellows Of Harvard College Barcoding systems and methods for gene sequencing and other applications
WO2018170515A1 (en) 2017-03-17 2018-09-20 The Broad Institute, Inc. Methods for identifying and modulating co-occurant cellular phenotypes
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US12297426B2 (en) 2019-10-01 2025-05-13 The Broad Institute, Inc. DNA damage response signature guided rational design of CRISPR-based systems and therapies
CA3157359A1 (en) 2019-10-10 2021-04-15 1859, Inc. Methods and systems for microfluidic screening
CN119654676A (zh) * 2022-08-23 2025-03-18 徕卡显微系统Cms有限公司 连接物、标志物、捕获构建体和数据存储装置
WO2024105001A1 (en) * 2022-11-18 2024-05-23 Leica Microsystems Cms Gmbh Method for sequencing
WO2025054490A1 (en) * 2023-09-08 2025-03-13 Rasyn, Llc Multi-omic analysis using beyond-poisson droplet loading

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007503984A (ja) * 2003-08-27 2007-03-01 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体種の電子的制御
JP2011509075A (ja) * 2007-12-21 2011-03-24 プレジデント アンド フェロウズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
JP2015528283A (ja) * 2012-08-14 2015-09-28 テンエックス・ジェノミクス・インコーポレイテッド マイクロカプセル組成物および方法

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US8592150B2 (en) * 2007-12-05 2013-11-26 Complete Genomics, Inc. Methods and compositions for long fragment read sequencing
US8367370B2 (en) * 2008-02-11 2013-02-05 Wheeler Aaron R Droplet-based cell culture and cell assays using digital microfluidics
US8808986B2 (en) * 2008-08-27 2014-08-19 Gen9, Inc. Methods and devices for high fidelity polynucleotide synthesis
WO2012019765A1 (en) * 2010-08-10 2012-02-16 European Molecular Biology Laboratory (Embl) Methods and systems for tracking samples and sample combinations
LT3305918T (lt) * 2012-03-05 2020-09-25 President And Fellows Of Harvard College Būdai, skirti epigenetinių sekų sudarymui
WO2014047561A1 (en) * 2012-09-21 2014-03-27 The Broad Institute Inc. Compositions and methods for labeling of agents

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007503984A (ja) * 2003-08-27 2007-03-01 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体種の電子的制御
JP2011509075A (ja) * 2007-12-21 2011-03-24 プレジデント アンド フェロウズ オブ ハーバード カレッジ 核酸の配列決定のためのシステムおよび方法
US20120220494A1 (en) * 2011-02-18 2012-08-30 Raindance Technolgies, Inc. Compositions and methods for molecular labeling
JP2015528283A (ja) * 2012-08-14 2015-09-28 テンエックス・ジェノミクス・インコーポレイテッド マイクロカプセル組成物および方法

Also Published As

Publication number Publication date
US20170028377A1 (en) 2017-02-02
AU2015247416A1 (en) 2016-11-03
EP3132072A1 (en) 2017-02-22
EP3132072B1 (en) 2019-12-04
CA2945794A1 (en) 2015-10-22
CN106661763A (zh) 2017-05-10
EP3132072A4 (en) 2017-11-29
WO2015161177A1 (en) 2015-10-22

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