JP2017511155A5 - - Google Patents

Download PDF

Info

Publication number
JP2017511155A5
JP2017511155A5 JP2017505732A JP2017505732A JP2017511155A5 JP 2017511155 A5 JP2017511155 A5 JP 2017511155A5 JP 2017505732 A JP2017505732 A JP 2017505732A JP 2017505732 A JP2017505732 A JP 2017505732A JP 2017511155 A5 JP2017511155 A5 JP 2017511155A5
Authority
JP
Japan
Prior art keywords
cas
bacterium
crrna
pam
deletion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017505732A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017511155A (ja
JP6621464B2 (ja
Filing date
Publication date
Priority claimed from GBGB1406968.6A external-priority patent/GB201406968D0/en
Application filed filed Critical
Publication of JP2017511155A publication Critical patent/JP2017511155A/ja
Publication of JP2017511155A5 publication Critical patent/JP2017511155A5/ja
Application granted granted Critical
Publication of JP6621464B2 publication Critical patent/JP6621464B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017505732A 2014-04-17 2015-04-16 欠失突然変異 Expired - Fee Related JP6621464B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1406968.6 2014-04-17
GBGB1406968.6A GB201406968D0 (en) 2014-04-17 2014-04-17 Deletion mutants
PCT/GB2015/051152 WO2015159086A1 (en) 2014-04-17 2015-04-16 Deletion mutations

Publications (3)

Publication Number Publication Date
JP2017511155A JP2017511155A (ja) 2017-04-20
JP2017511155A5 true JP2017511155A5 (enExample) 2018-05-31
JP6621464B2 JP6621464B2 (ja) 2019-12-18

Family

ID=50928940

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2017505732A Expired - Fee Related JP6621464B2 (ja) 2014-04-17 2015-04-16 欠失突然変異

Country Status (9)

Country Link
US (1) US10550396B2 (enExample)
EP (1) EP3132035B8 (enExample)
JP (1) JP6621464B2 (enExample)
KR (1) KR20170020320A (enExample)
CN (1) CN106460001A (enExample)
BR (1) BR112016023559A2 (enExample)
CA (1) CA2945572A1 (enExample)
GB (4) GB201406968D0 (enExample)
WO (1) WO2015159086A1 (enExample)

Families Citing this family (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013066438A2 (en) 2011-07-22 2013-05-10 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
DK2986729T3 (en) 2013-04-16 2018-10-29 Regeneron Pharma TARGETED MODIFICATION OF ROOT THROUGH
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
WO2015066119A1 (en) 2013-10-30 2015-05-07 North Carolina State University Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
WO2015088643A1 (en) 2013-12-11 2015-06-18 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a genome
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
US10787654B2 (en) 2014-01-24 2020-09-29 North Carolina State University Methods and compositions for sequence guiding Cas9 targeting
EP4063503A1 (en) 2014-02-11 2022-09-28 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
EP3129490A4 (en) 2014-04-08 2017-10-04 North Carolina State University Methods and compositions for rna-directed repression of transcription using crispr-associated genes
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
US10450584B2 (en) 2014-08-28 2019-10-22 North Carolina State University Cas9 proteins and guiding features for DNA targeting and genome editing
CA2968440A1 (en) 2014-11-21 2016-05-26 Regeneron Pharmaceuticals, Inc. Methods and compositions for targeted genetic modification using paired guide rnas
KR20200091499A (ko) 2015-05-06 2020-07-30 스니프르 테크놀로지스 리미티드 미생물 개체군 변경 및 미생물군 변형
WO2016196361A1 (en) 2015-05-29 2016-12-08 North Carolina State University Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids
JP7051438B2 (ja) 2015-06-15 2022-04-11 ノース カロライナ ステート ユニバーシティ 核酸およびｒｎａに基づく抗菌剤の効率的な送達のための方法および組成物
WO2017058751A1 (en) 2015-09-28 2017-04-06 North Carolina State University Methods and compositions for sequence specific antimicrobials
SG10202104041PA (en) 2015-10-23 2021-06-29 Harvard College Nucleobase editors and uses thereof
WO2017112620A1 (en) 2015-12-22 2017-06-29 North Carolina State University Methods and compositions for delivery of crispr based antimicrobials
AU2017260714B2 (en) * 2016-05-01 2023-10-05 Duane CHUNG Harnessing heterologous and endogenous CRISPR-Cas machineries for efficient markerless genome editing in clostridium
US20170332610A1 (en) 2016-05-20 2017-11-23 Regeneron Pharmaceuticals, Inc. Methods for breaking immunological tolerance using multiple guide rnas
GB201609811D0 (en) 2016-06-05 2016-07-20 Snipr Technologies Ltd Methods, cells, systems, arrays, RNA and kits
ES2915562T3 (es) 2016-06-24 2022-06-23 Univ Colorado Regents Métodos para generar bibliotecas combinatorias con código de barras
KR20250103795A (ko) 2016-08-03 2025-07-07 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
SG11201903089RA (en) 2016-10-14 2019-05-30 Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
EP3592381A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Cancer vaccine
US11898179B2 (en) 2017-03-09 2024-02-13 President And Fellows Of Harvard College Suppression of pain by gene editing
WO2018165629A1 (en) 2017-03-10 2018-09-13 President And Fellows Of Harvard College Cytosine to guanine base editor
WO2018176009A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
GB201712733D0 (en) 2017-08-08 2017-09-20 Snipr Tech Ltd Methods & cells
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
US10760075B2 (en) 2018-04-30 2020-09-01 Snipr Biome Aps Treating and preventing microbial infections
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020072250A1 (en) 2018-10-01 2020-04-09 North Carolina State University Recombinant type i crispr-cas system and uses thereof for genome modification and alteration of expression
US10711267B2 (en) 2018-10-01 2020-07-14 North Carolina State University Recombinant type I CRISPR-Cas system
US12264330B2 (en) 2018-10-01 2025-04-01 North Carolina State University Recombinant type I CRISPR-Cas system and uses thereof for killing target cells
WO2020072253A1 (en) 2018-10-01 2020-04-09 North Carolina State University Recombinant type i crispr-cas system and uses thereof for screening for variant cells
US11851663B2 (en) 2018-10-14 2023-12-26 Snipr Biome Aps Single-vector type I vectors
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US11142751B2 (en) 2019-03-07 2021-10-12 Auburn University CRISPR-cas system for Clostridium genome engineering and recombinant strains produced thereof
EP3942043A2 (en) 2019-03-19 2022-01-26 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
WO2020214842A1 (en) 2019-04-17 2020-10-22 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
FR3096373B1 (fr) * 2019-05-24 2024-09-13 Ifp Energies Now Outil genetique optimisé pour modifier les bacteries
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
BR112022022603A2 (pt) 2020-05-08 2023-01-17 Broad Inst Inc Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla
GB202209518D0 (en) 2022-06-29 2022-08-10 Snipr Biome Aps Treating & preventing E coli infections

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NO323345B1 (no) 2002-01-07 2007-04-02 Norwegian Inst Of Fisheries An Forbedring av fiskeprodukter og fôr
GB0612301D0 (en) 2006-06-21 2006-08-02 Morvus Technology Ltd DNA molecules and methods
WO2008040387A1 (en) 2006-10-03 2008-04-10 Metabolic Explorer Process for chromosomal integration and dna sequence replacement in clostridia
AU2008335178A1 (en) 2007-12-10 2009-06-18 Ablexis Llc Methods for sequential replacement of targeted region by homologous recombination
GB0802842D0 (en) 2008-02-15 2008-03-26 Univ Nottingham Synthetic operon construction in clostridia
WO2009137778A2 (en) 2008-05-08 2009-11-12 Northwestern University Methods and compositions for genetically engineering clostridia species
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
WO2010127111A1 (en) 2009-04-30 2010-11-04 The Trustees Of Comlumbia University In The City Of New York In vivo assembly of dna via homologous recombination
WO2013133882A2 (en) 2011-12-16 2013-09-12 University Of Delaware Recombinant clostridium organism and method for isolation of double-crossover allelic exchange mutants
WO2013141680A1 (en) * 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
CN114634950A (zh) 2012-12-12 2022-06-17 布罗德研究所有限公司 用于序列操纵的crispr-cas组分系统、方法以及组合物
US10612043B2 (en) * 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
ES2670531T3 (es) * 2013-05-29 2018-05-30 Cellectis S.A. Un método para producir una escisión de ADN precisa utilizando la actividad nickasa de Cas9
ES2844174T3 (es) 2013-09-18 2021-07-21 Kymab Ltd Métodos, células y organismos
EP4063503A1 (en) 2014-02-11 2022-09-28 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering

Similar Documents

Publication Publication Date Title
JP2017511156A5 (enExample)
Song et al. CRISPR-Cas9D10A nickase-assisted genome editing in Lactobacillus casei
McAllister et al. CRISPR genome editing systems in the genus Clostridium: a timely advancement
JP6621464B2 (ja) 欠失突然変異
Huang et al. Development of a RecE/T‐assisted CRISPR–Cas9 toolbox for Lactobacillus
Li et al. CRISPR‐based genome editing and expression control systems in Clostridium acetobutylicum and Clostridium beijerinckii
US9988637B2 (en) Cas9 plasmid, genome editing system and method of Escherichia coli
Wang et al. Markerless chromosomal gene deletion in Clostridium beijerinckii using CRISPR/Cas9 system
US11946067B2 (en) Optimized genetic tool for modifying Clostridium bacteria
Ehsaan et al. Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824
CN101528930B (zh) 用于在梭菌属中进行染色体整合和dna序列替换的方法
EP3778867A1 (en) Phage resistant lactic acid bacteria
JP6473510B2 (ja) ビブリオで汎用される遺伝子ノックアウト用の自殺ベクターおよびその利用
Zhang et al. Markerless genome editing in Clostridium beijerinckii using the CRISPR-Cpf1 system
Pyne et al. Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme
JP2011512153A5 (enExample)
Tejada et al. Prevention and cure of murine C. difficile infection by a Lachnospiraceae strain
Brumwell et al. Slicer: A seamless gene deletion method for Deinococcus radiodurans
JP7555599B2 (ja) 遺伝子的に改変されたｃｌｏｓｔｒｉｄｉｕｍ細菌の、調製およびその使用。
CN105483034A (zh) 一种转换酵母交配型的方法
Patinios et al. Multiplex genome engineering in Clostridium beijerinckii NCIMB 8052 using CRISPR-Cas12a
JP7661241B2 (ja) 細菌を改変するための最適化された遺伝子ツール
CN112029699B (zh) 一种基于内源CRISPR-Cas系统的丁酸梭菌基因编辑系统及其应用
Little et al. The butanol producing microbe Clostridium beijerinckii NCIMB 14988 manipulated using forward and reverse genetic tools
Vento et al. Genome editing with Cas9 in lactobacilli