JP2017176167A - Filter for germ-free animal breeding device, sterilization can, and conveyance can - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an improved filter, a sterilization can, and a conveyance can that can be used for a germ-free animal breeding device.SOLUTION: There is provided a filter for a germ-free animal breeding device including a HEPA filter protected by a protective agent or a protective frame. There is also provided a sterilization can and a conveyance can for a germ-free animal breeding device including a HEPA filter, and a germ-free animal breeding system. There is further provided a conveyance can for a germ-free animal breeding device, in which at least one of upper and lower faces is made of transparent polycarbonate, and which has a filter on the side face.SELECTED DRAWING: Figure 1

Description

  The present invention relates to a filter for an aseptic animal breeding apparatus, a sterilized can and a transport can.

  Conventionally, aseptic animal breeding apparatuses have been used for the purpose of breeding sterile animals and the like. The aseptic animal breeding apparatus is also called an isolator, a vinyl isolator or the like.

  The vinyl isolator usually includes an air supply port, a breeding room, and an exhaust port. The air supplied from the air supply port needs to be sterile, and a filter is generally used for that purpose. The filter removes microorganisms and viruses in the outside air to realize sterility. In addition, the aseptic animal breeding apparatus is used together with a sterilized can in order to take in and out the contents. The sterilization can is attached to the device so that the contents can be placed in a sterile animal breeding device or the content of the device can be transferred to the sterilization can while maintaining the sterilized state. is there. Furthermore, aseptic animal breeding devices are used with transport cans to transport and remove sterile animals. The transport can can still be attached to the device so that aseptic animals can be transferred to and removed from the device while maintaining aseptic conditions.

  As a filter medium for a filter for an aseptic animal breeding apparatus, a filter medium FG-50 (manufactured by American Air Filter) has been traditionally used in the industry. This has been consistently used since the Showa 30s since the introduction of vinyl isolator technology from the United States. FG-50 has been used for sterilized cans and transport cans as well.

  This FG-50 filter has come to be used for aseptic animal breeding, as a filter for a sterile steel tank, in order to equalize the heat of nichrome wire with glass wool, FG-50 was wrapped with nichrome wire and used. This is because sterility is maintained even if a power failure occurs. Since then, vinyl isolators continue to use FG-50 as a filter medium. It was not selected based on dust efficiency (dust collection efficiency).

  Thereafter, high performance filters (also referred to as HEPA filters) were developed, and many studies on dust (clean rooms, etc.) have been conducted. This is used as a filter for a clean room, and is used for manufacturing semiconductors, liquid crystal parts, pharmaceuticals, foods, and the like that require high performance. Some air purifiers use this filter. Some home vacuum cleaners have adopted this filter from the viewpoint of preventing allergies from house dust. However, in spite of being diverted to such various uses, such a high-performance filter has not been studied in the aseptic animal industry, and the proven FG-50 continues to be used. Since the HEPA filter is easily damaged and difficult to handle as it is, it is generally provided in a state of being incorporated in a protective structure, for example, in the form of a cartridge.

  When a large isolator having a volume 10 times that of a normal isolator was developed, an attempt was made to use a high-performance filter. However, high-performance filters can only be obtained as a folded product for indoor use, and the pressure loss of the filter medium itself is expected to be high, and it is easy to be damaged. There was a problem, and it was not possible to use it for a normal small isolator.

  Since the dust collection efficiency of FG-50 is not so high, some filter researchers questioned its performance. However, when FG-50 is used in quadruplicity, sterility can be realized in practice, and viruses and bacteria are not detected.

  There is a need for improved filters for vinyl isolators. Further, the FG-50 filter is relatively expensive, and a cheaper filter for a vinyl isolator is demanded. The same applies to sterilized cans and transport cans for aseptic animal breeding equipment.

  Conventional transport cans (so-called paper cans) made of tin on the upper and lower surfaces have a problem that the contents cannot be seen from the outside. In particular, regarding the transportation of sterile animals, it is necessary to check the contents at the time of censorship, but there is a problem that the aseptic environment cannot be maintained if the upper and lower surfaces are opened.

  An object of the present invention is to provide a filter, a sterilization can, and a transport can for an aseptic animal breeding apparatus that solve the above-described problems.

  In the development and domestic production of vinyl isolators, the present inventors have examined various materials and parts, but there is no single equivalent, and currently imported products continue to be used. High-performance filters are generally only available as indoor folded products.

  Therefore, the present inventors have succeeded in obtaining a filter material of a specially designed high performance filter. When this and a performance test with FG-50 (quadruple) were conducted, it was found that the HEPA filter medium had a slight reduction in ventilation, but the dust collection efficiency was dramatically improved compared to FG-50. This completed the present invention. Furthermore, the present inventors conducted a performance test with FG-50 using a filter material of a specially designed high-performance filter for sterilized cans and transport cans, and the dust collection efficiency was dramatically improved. I understood. This completed the present invention. Furthermore, the present inventors have developed a transport can whose contents can be confirmed from the outside by using either or both of the upper and lower surfaces of the transport can as a polycarbonate material, and also developed a combination of this with HEPA. did. That is, the present invention includes the following embodiments.

[1] A filter for an aseptic animal breeding apparatus provided with a flat HEPA filter whose both surfaces are protected by a protective agent.
[2] A filter for aseptic animal breeding equipment, comprising a columnar HEPA filter protected by a cylindrical protective frame.
[3] A sterile animal breeding device comprising the filter according to 1 or 2.
[4] A sterilized can for aseptic animal breeding equipment equipped with a HEPA filter.
[5] A transport can for an aseptic animal breeding apparatus, wherein at least one of the upper and lower surfaces is made of transparent polycarbonate and has a filter on a side surface.
[6] The transport can according to 5, wherein the filter is a HEPA filter.
[7] The transport can according to 5, wherein the filter is an FG-50 filter.
[8] A sterile animal breeding system comprising the apparatus according to 3, and the sterilized can according to 4, and / or the transport can according to 5, 6 or 7.
[9] A sterile animal breeding method using the filter according to 1 or 2, the device according to 3, the sterilized can according to 4, the transport can according to 5, 6 or 7, or the system according to 8.

  This specification includes the disclosure of Japanese Patent Application No. 2016-063934, which is the basis of the priority of the present application.

  By using the filter of the present invention, ventilation can be performed with high dust collection efficiency. The filter and the sterilized can of the present invention can be used for an aseptic animal breeding apparatus. The transport can of the present invention can be used for transporting aseptically bred animals while allowing the contents of the animals to be confirmed from the outside.

It is a figure of the planar filter of this invention. It is a figure of the cylindrical filter of this invention. It is a figure of the components of a sterilization can. Reference numeral 1 denotes a main body (body portion). 2 is a filter-retaining screen. 3 is a legged band. 4 is a fastening band for the same part. 5 is a bottom filter holding ring. 6 is a drawer net. Reference numeral 7 denotes an attached screw. It is a figure of a sterilization can body. It is a completion drawing of a sterilization can. It is a figure of winding of a filter. It is a figure of fixation of the filter with a polytape. It is a figure of adhesion of A and B part in a polytape. It is a figure of attachment of a protection net. It is a figure of attachment of a ring. It is a figure of the assembly of a bottom part. It is a figure of cutting of a filter. It is sectional drawing of a transport can. It is a perspective view of the main body (before filter attachment) of a transport can. 2 is an upper or lower paper can part, and 3 is a wire mesh part.

  In certain embodiments, the present invention provides a filter for a sterile animal breeding device. The filter of the present invention may be a planar HEPA filter whose both surfaces are protected with a protective agent, or a columnar HEPA filter protected by a cylindrical protective frame. Here, “both surfaces” of the planar filter refers to both the surface on the air duct side and the surface on the isolator air inlet side.

The HEPA (High Efficiency Particulate Arrestance) filter is a filter that can capture particles with high efficiency. According to the JIS Z 8122 standard, the particle collection rate is 99.97% or more for particles with a rated air volume of 0.3 μm. And an air filter having an initial pressure loss of 245 Pa or less ”. That is, the filter of the present invention conforms to this standard. The HEPA filter of the present invention has a collection efficiency of 99.97% or more, 99.98% or more, for example 99.99% or more, and an initial pressure loss of 245 Pa with respect to particles having a rated air volume of 0.3 μm. In the following, 200 Pa or less, 150 Pa or less, 100 Pa or less, 50 Pa or less, for example, 45 Pa or less can be used. The performance can be evaluated at a treatment air volume of 0.140 to 0.150 m ³ / min, for example, 0.147 m ³ / min. FG-50 is a neutral filter having glass fibers, and its particle collection rate is 95% or more with respect to particles having a rated air volume of 0.5 μm. In one embodiment, when referring to an FG-50 filter, this is synonymous with a neutral filter having glass fibers having a particle collection rate of 95% or more with respect to particles having a rated air volume of 0.5 μm (also an implementation). If the neutral filter in the form has an initial pressure loss, it is the same as that of FG-50 manufactured by American Air Filter).

  In certain embodiments, the shape of the filter media portion of the filter of the present invention can be flat. The shape of the plane may be circular or polygonal. Preferably the planar filter is circular. In an embodiment where the filter of the present invention is planar, the HEPA filter portion can be protected on both sides by a protective agent. The protective agent is sometimes called a prefilter. The filter material of the prefilter can be glass mat, glass fiber, glass wool, rock wool or the like. The filter medium of the HEPA filter can be glass paper.

  In a certain embodiment, the shape of the filter medium part of the filter of this invention can be made into a column shape. In the embodiment in which the filter of the present invention is cylindrical, the filter medium portion of the filter can be protected on the outer side by a cylindrical protective frame. The filter medium of the HEPA filter can be glass paper. The protective frame can be made of aluminum, plastic, stainless steel, resin, or the like. The protective frame and the HEPA filter can be bonded with an adhesive. As the adhesive, various resins such as a urethane resin can be used. The cylindrical filter can have a packing for connection. The packing can be a silicone gasket.

The cylindrical filter of the present invention has a collection efficiency of 99.97% or more, 99.98% or more with respect to particles having a rated air volume of 0.3 μm when evaluated with the HEPA filter protected by a protective frame. For example, a material having a pressure loss of 99.99% or more and an initial pressure loss of 245 Pa or less, 200 Pa or less, 150 Pa or less, 100 Pa or less, 50 Pa or less, for example 45 Pa or less can be used. The performance can be evaluated at a treatment air volume of 0.140 to 0.150 m ³ / min, for example, 0.147 m ³ / min.

  The size and dimensions of the filter are not particularly limited as long as they can be used in a normal aseptic breeding apparatus. The planar filter can be assembled as a disk filter, or may be assembled as a square filter or the like. Multiple filters can also be used in series or in parallel.

  The performance evaluation of the filter can be performed by measuring the removal rate of 0.3 μm particles at the rated air volume and the pressure loss. The air can be blown by a normal isolator blower. For convenience, the blower wind tunnel side of the filter is called the filter primary side, and the isolator air inlet side is called the filter secondary side. The dust collection efficiency is evaluated by measuring the number of dust on the primary and secondary sides of the filter.

  The filter of the present invention can be used for filtering air supplied to a vinyl isolator. The vinyl isolator may be a normal one or a small one.

  In one embodiment, the present invention provides a sterilization can for a sterile animal breeding device. The sterilization can is made of a material that can be sterilized by autoclave, such as stainless steel. At least one of the outer surfaces of the sterilization can includes an openable / closable mechanism for attaching the sterilization can to the aseptic breeding apparatus. When the sterilized can is attached to the aseptic breeding apparatus, the contents such as food and water can be transferred into the breeding apparatus, and the filth and waste in the breeding apparatus can be transferred to the sterilized can. The sterilization can of this invention is equipped with the HEPA filter protected with the protective agent. The sterilization can of the present invention may have any shape as long as it can be attached to an aseptic breeding apparatus. One or more of the outer surfaces of the sterilization can other than the surface to be attached to the aseptic breeding apparatus have a HEPA filter.

  In one embodiment, the present invention provides a transport can for a sterile animal breeding device. The transport can is made of a material that can be autoclaved, such as stainless steel. At least one of the outer surfaces of the transport can includes an openable and closable mechanism for attaching the transport can to the aseptic breeding apparatus. The openable / closable mechanism can be a lid. When the transport can is attached to the aseptic breeding apparatus, the animal in the breeding apparatus can be transferred to the transport can, or the breeding animal in the transport can can be transferred to the (another) breeding apparatus. The transport can of the present invention includes a filter protected with a protective agent. The filter can be a HEPA filter or an FG-50 filter. The protective agent may be a cloth material or a prefilter such as glass mat, glass fiber, glass wool, rock wool or the like. The transport can of the present invention may have any shape as long as it can be attached to an aseptic breeding apparatus, for example, a cylinder, a cylinder, a quadrangular prism, a quadrangular cylinder, a polygonal cylinder, a polygonal cylinder, an oblique cylinder, an oblique cylinder, an oblique cylinder, The shape may be a slanted cylinder or the like. In an embodiment, the shape of the transport can is a cylindrical shape. The transport can has a size and dimensions that can accommodate one or more domestic animals. At least one of the outer surfaces of the transport can other than the surface to be attached to the aseptic breeding apparatus is provided with a filter protected with a protective agent. The filter can be a HEPA filter or an FG-50 filter.

  In one embodiment, when the transport can of the present invention is a cylindrical shape, at least one of the upper and lower surfaces is made of polycarbonate, preferably transparent polycarbonate. In one embodiment, the polycarbonate is a flat and single piece with no irregularities on the surface. At least one of the upper and lower surfaces is removable from the transport can body for placing the animal in a sterile animal breeding device.

  In one embodiment, the transport can of the present invention is cylindrical and has the following configuration. That is, a polycarbonate lid 1 may be provided on one or both of the upper and lower surfaces of the transport can body, and the one or both lid portions are removable. The side surface has a filter 4, which is protected with a protective agent 5. The protective agent may be a cloth material or a prefilter. The top and bottom of the filter part can be paper (paper can part 2). The paper material above the filter portion and the paper material below are connected to each other by a member 3 that protects the inside of the filter. The member 3 that protects the inside of the filter can be a wire mesh or punching metal. The wire mesh can be made of stainless steel. In some embodiments, the wire mesh is not made of aluminum. The filter 4 can be a HEPA filter or an FG-50 filter. After attaching the protective agent 5 to protect the filter 4, the protective agent can be adhered and fixed by a fixing means 6 such as glue or adhesive tape. See, for example, FIGS.

  The sterilized can can be attached to the aseptic animal breeding apparatus by the following method, for example. (i) Remove the vinyl isolator outer cap. (ii) Disinfect the steel lock and connecting sleeve. (Iii) Attach a connecting sleeve to the steel reel and fix it with vinyl tape (sealing). (Iv) Disinfect the other part of the connecting sleeve and the Lumirror film part of the sterilization can. (V) Connect the connecting sleeve and the sterilized can and fix them with vinyl tape (sealed). Disinfection can be performed, for example, by spraying with expeller or peracetic acid.

  The transport can can be attached to the aseptic animal breeding apparatus by the following method, for example. (1) Disinfect transport cans and sleeves. (2) Attach a sleeve to the sterilized transport can and seal it with vinyl tape. (3) Remove the outer cap of the isolator. (4) Disinfect the steel lock. (5) Disinfect the inside and outside of the sleeve attached to the transport can. (6) Attach the open side of the sterilized sleeve to the steel lock and seal it with vinyl tape. (7) Gently push the transport can into the steel reel side to release air from the sleeve. (8) Disinfect the inside of the sleeve via the sleeve spray port. Disinfection can be performed by means such as a spray gun. Disinfection is performed until the sleeve becomes cylindrical. (9) Remove the spray gun, gently push the inside of the transport can into the steel lock side, and remove the disinfectant from the sleeve. (10) Repeat (8) above, put a rubber stopper and leave it for several hours. (11) Confirm that there is no dent in the sleeve, and remove the cap in the vinyl isolator. (12) Push the transport can into the isolator through the steel reel lock. (13) Turn over the sleeve around the cap sealing the transport can and expose the cap of the transport can. (14) Take up the tape fixing the cap of the transport can, and then remove the cap of the transport can. (15) Move the animal from the transport can into the device, or move the animal in the device to the transport can. Then, an inner cap is attached to the steel lock and fixed with a rubber band. (16) Remove the sleeve reel tape on the sleeve and remove the transport can. (17) Disinfect the steel lock and outer cap, attach the outer cap to the steel lock, and seal with vinyl tape. (18) Push the outer cap into the steel lock, vent the air inside the steel lock, and spray the disinfectant. (19) Push the inner cap into the steel lock again, remove the air inside the steel lock, spray disinfectant to the extent that the outer cap swells, and seal it with a rubber stopper. (20) After spraying the disinfectant with a means such as a spray gun, put a rubber stopper and leave the inner cap and outer cap inflated for several hours.

  In one embodiment, the present invention provides a sterile animal breeding system for a sterile animal breeding device and having a sterile can and / or a transport can. In one embodiment, the present invention provides a method for raising aseptic animals using the filter, aseptic animal breeding apparatus, sterilized can, transport can or system of the present invention.

  The filter material part of the filter was obtained from NITTA Corporation. Details of the specifications are described in FIG. 1 and FIG.

The disk filter was assembled by the following procedure.
1. Prepare filter media for the disk filter.
2. Prepare to change the disk filter. For this purpose, a disk filter container, a filter medium, and a filter casing are prepared.
3. Place the filter medium on one side of the filter casing. At that time, the filter medium is superimposed on the center of the filter casing. When a filter protected as a filter medium is used, a filter already protected by a protective agent is used as the filter medium at this stage.
4). The other filter casing is overlaid on the filter casing overlaid with the filter medium.
5. Install the disc filter clamping ring.
6). Tighten the screw of the tightening ring with a tool.

  Be careful when handling the filter because the HEPA filter will be damaged if it is pressed or bent strongly.

[Example 1]
Filter for aseptic animal breeding equipment Filter medium, FG-50 (quadruple), HEPA filter medium, new HEPA, cylindrical HEPA are incorporated in the air supply disk filter, exhaust is incorporated FG-50 (quadruple) in the exhaust disk filter . Note that FG-50 (quadruple) is a conventionally used filter medium. The HEPA filter medium in the table is a HEPA filter having no protective agent. The new HEPA in the table is a flat HEPA filter with both sides protected by a protective agent. Cylindrical HEPA is a columnar HEPA filter protected by a cylindrical frame.

  Air was blown using an isolator blower under five voltage conditions of 25V, 40V, 60V, 100V, and 130V. The wind speed, temperature, humidity, pressure of the blower wind tunnel (fluter primary side) and the number of dust at the isolator air inlet (filter secondary side) were continuously measured 11 times per minute. The measurement was performed three times for each voltage, and the average values were compared. The results are shown in Table 1 below.

  As a result, 25V air flow was omitted from the results because the air volume was small. Comparing the average of four conditions of 40 V or higher with FG-50, the air supply air speed was 84% for HEPA filter medium, 81% for new HEPA, and 116% for cylindrical HEPA. The wind tunnel pressure was 100% for HEPA filter media, 101% for new HEPA, and 99% for cylindrical HEPA. The 0.3μ number was 0.4% HEPA filter medium, 0.3% new HEPA, and 0.6% cylindrical HEPA.

As described above, it has been found that the HEPA filter medium significantly improves the dust collection efficiency, although the ventilation amount is slightly reduced as compared with FG-50. In consideration of handling, the new HEPA in which the HEPA filter was protected with a protective agent gave the same results as the unprotected HEPA filter medium. Further, in the filter (cylindrical HEPA) in which the HEPA filter medium was woven into a columnar shape and protected with a cylindrical protective frame, the ventilation amount was improved and the dust collection efficiency was also improved.
The filter of the present invention had unexpectedly low pressure loss.

[Example 2]
Sterilized can for aseptic animal breeding device A sterile can for aseptic animal breeding device was assembled according to the following procedure. The dimensions are an example.
Parts of a sterilization can (an example is shown in FIG. 3)
1 Body (body) Opening: Φ315 × 170 mm (Stainless steel 0.7 mm)
Bottom: Φ315 × 170 mm (Stainless steel 0.7 mm)
Body: Φ315 × 360 mm (Stainless steel 10 mm mesh, wire diameter 1.5 mm)
2 Filter filter Ami Stainless steel (10mm mesh, wire diameter 1.5mm) 450 × 1000 mm
3 Band with leg made of stainless steel (width 30mm × 0.7mm)
4 Tightening band for stainless steel (width 30mm × 0.7mm)
5 Bottom filter retaining ring Made of stainless steel (width 30 mm x 0.7 mm) 12 holes with 10 mm mesh mesh screws 6 drawer nets Stainless steel: 213 mm x 583 mm x 1.3 t (Φ6 mm hole processed)
7 Attached screw Attached screw: For band; M6 x 70mm (with wing nut)
For bottom filter retaining ring: M6 wing nut

Assembling the sterilization can Prepare the sterilization can body. For example, as shown in FIG. 6, an American air filter FG-50 (4,000 mm × 420 mm) is wound about 4 turns around the body of a sterilized can to make it quadruple. Or, instead of FG-50 quadruple winding, a new HEPA filter (filter with a HEPA filter medium sandwiched between them) is wound in a single layer. Wrap gently and slowly to avoid damaging the filter.
When viewed from the direction perpendicular to the longitudinal direction of the sterilization can, the center of the filter is wrapped with polytape and fixed (see FIG. 7). Next, both ends are brought into close contact with the sterilization can with a poly tape (see FIG. 8). When fixing both ends, the tape should be fully adhered (if not enough, there is a possibility of infection).
Place one SUS plate (Steel Special Use Stainless) on the end of the filter, and wrap a net from there (see Fig. 9). Wrap around the sterilization can for about one turn. Both ends of the protective mesh wound around the sterilization can are sandwiched between SUS plates and fixed with a ring (band) (see FIG. 10). Close the bottom of the sterile can with a filter. A protective net is placed on the top and fixed with a nut (see FIG. 11). Cut off the excess filter protruding from the edge of the protective mesh (see FIG. 12). Cut out about 3-5mm from the sterilized can body to leave the filter. Each figure is only an example for explaining assembly, and the present invention is not limited to the shown configuration.

  The filter performance was measured by attaching a blower to the opening in the discharge direction so that the inside of the can has a negative pressure. Air blowing was performed at blower voltages of 40V, 60V, 80V, 100V, and 120V. The wind speed, temperature, humidity, pressure inside the transport can, and the number of 0.3 μm particles in 0.1 cf were measured continuously 11 times a minute. The measurement was performed three times for each voltage, and the average values were compared. The results are shown in Table 2 below.

  As a result, as for the sterilized can filter shown in Table 2, the sterilized can with the new HEPA filter has a gap area calculated from the air volume of 79.6% compared to that of the sterilized can with FG-50, and the can internal pressure is 113.2%, the number of 0.3 μm particles in the can was 0.26%, and dust invasion prevention efficiency was significantly improved. That is, as compared with FG-50, the sterilization can having the HEPA filter dramatically improved the dust collection efficiency.

[Example 3]
Transport can for aseptic animal breeding device A transport can for aseptic animal breeding device was assembled by the following procedure.
A transport can body having two upper and lower can portions fixed by a cylindrical metal net is prepared. An American air filter FG-50 is wound around the body part about twice around the body part between the upper and lower can parts, and is doubled. Or, instead of double winding of FG-50, a new HEPA filter (a filter in which a HEPA filter medium is sandwiched by an emulent) is wound in a single layer. Wrap gently and slowly to avoid damaging the filter. Protect the outside of the filter with a cloth material. The inside of the filter is protected by a metal net. Fix the wound fabric material with polytape or the like while keeping it in close contact.

  The filter performance was measured by attaching a blower to the opening in the discharge direction so that the inside of the can has a negative pressure. Air blowing was performed at blower voltages of 40V, 60V, 80V, 100V, and 120V. The wind speed, temperature, humidity, pressure inside the transport can, and the number of 0.3 μm particles in 0.1 cf were measured continuously 11 times a minute. The measurement was performed three times for each voltage, and the average values were compared. The results are shown in Table 3 below.

  As a result, regarding the transport can filter shown in Table 3, the transport can having the new HEPA has a gap area calculated from the air volume of 86.3% compared to that of the transport can having FG-50, and the can internal pressure is 102. 0.4%, the number of 0.3 μm particles in the can was 0.00%, and the dust prevention efficiency was significantly improved. That is, as compared with FG-50, the transport can having the HEPA filter dramatically improved the dust collection efficiency.

  The filter medium of the present invention can be used in an aseptic animal breeding apparatus. The sterilized can of the present invention can be used for aseptic animal breeding. The transport can of the present invention can be used for transport of aseptically bred animals.

DESCRIPTION OF SYMBOLS 1 Cover 2 Paper can part 3 The member which protects the inside of a filter 4 Filter 5 Protection agent 6 Fixing means

Claims (9)

  A filter for an aseptic animal breeding apparatus, comprising a flat HEPA filter whose both surfaces are protected by a protective agent.   A filter for aseptic animal breeding equipment, comprising a columnar HEPA filter protected by a cylindrical protective frame.   A sterile animal breeding apparatus comprising the filter according to claim 1.   A sterilized can for aseptic animal breeding equipment equipped with a HEPA filter.   A transport can for an aseptic animal breeding apparatus, wherein at least one of the upper and lower surfaces is made of transparent polycarbonate and has a filter on a side surface.   The transport can according to claim 5, wherein the filter is a HEPA filter.   The transport can according to claim 5, wherein the filter is an FG-50 filter.   A sterile animal breeding system comprising the apparatus according to claim 3 and the sterilized can according to claim 4 and / or the transport can according to claim 5, 6 or 7.   Use a filter according to claim 1 or 2, a device according to claim 3, a sterilization can according to claim 4, a transport can according to claim 5, 6 or 7, or a system according to claim 8. Aseptic animal breeding method.

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