JP2017109942A - Composition for promoting knee joint function improvement action and skeletal muscle function enhancement action by resistance training, comprising oleanane-type triterpene as active ingredient, and using no training equipment - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for promoting a knee joint function improvement action by resistance training using no training equipment, and a composition for promoting a skeletal muscle function enhancement action by resistance training using no training equipment.SOLUTION: The problems is solved by employing oleanane-type triterpene as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a composition for promoting the knee joint function improving action by resistance training without using a training device containing oleanane type triterpene as an active ingredient. The present invention also relates to a composition for promoting skeletal muscle function enhancing action by resistance training without using a training device containing oleanane type triterpene as an active ingredient.

Vertebrates including humans are at risk of developing motor organ syndrome (also known as locomotive syndrome) due to aging and lifestyle habits. The musculoskeletal syndrome was defined by the Japanese Society of Clinical Orthopedic Surgery as “a state in which the risk of needing long-term care due to musculoskeletal decline or disability” increases. There are many cases of musculoskeletal syndrome, such as traumatic arthropathy due to traumatic causes such as injury, rheumatoid arthritis which is a kind of autoimmune disease, degenerative spondylosis due to degeneration or deformation of intervertebral disc, muscle with aging These include reduction, neuropathy due to stenosis, bone abnormalities due to fractures and osteoporosis, osteoarthritis, and the like.
Almost all joints of vertebrates have cartilage, which is important for providing smooth joint movement such as joint flexibility and compressibility. Many symptoms are caused, including decreased sex, inflammation of joints and surrounding tissues, and pain. Examples of such symptoms include osteoarthritis. Osteoarthritis tends to develop in weight-bearing joints such as knees, hips and ankles. Although traumatic joint damage can be the cause, most are due to cartilage wear and degeneration and aging with age.
The International Society for Arthritis recommends resistance training as a coping therapy for people with knee pain (Non-patent Document 1). Resistance training is an isometric exercise and / or an isotonic exercise that applies a load (resistance) to local or whole body muscle groups to improve skeletal muscle functions such as muscle strength, muscle power, and muscle endurance. Here, the isometric exercise is a static muscular strength strengthening method characterized by applying a certain load to the muscle without moving the joint, and contracting without changing the length of the muscle. Isotonic exercise is a dynamic muscle strength strengthening method characterized by moving a joint, applying a certain load to the muscle, and contracting while changing the length of the muscle.
Resistance training is broadly divided into a mode using a training device and a mode not using such a training device. The former is a facility use type such as a training gym or a medical institution. The latter is a facility-free type that does not require any place, and has the advantage that it can be easily done without spending money at home or in a park.
In an arthritis patient or a victim or a potential victim (hereinafter referred to as an arthropathy patient) having knee pain, a light load resistance training that can be performed without pain as much as possible is preferable, for example, self-weight exercise, tube exercise, Examples include martial arts and body exercises, and underwater exercises. As a result, when the muscles around the joint are strengthened and the force to support the joint is increased, the sense of stability of the joint is increased, so the burden on the joint is reduced, and as a result, the pain of the joint can be alleviated.

Triterpenes are a group of compounds widely distributed in the plant kingdom, and are a group of compounds classified into oleanane type triterpenes and ursan type triterpenes. The oleanane type triterpenes include maslinic acid, oleanolic acid, and the like, and the ursan type triterpenes include ursolic acid, corosolic acid, tormic acid, and the like.
Plant-derived oleanane-type triterpenes have recently attracted attention as functional components having physiological activity, and maslinic acid inhibits colon cancer (non-patent document 2), skin whitening (patent document 1), and osteoclast differentiation inhibition. And bone resorption activity effect (Patent Document 2), obesity prevention effect (Patent Document 3), composition containing indigestible dextrin, corosolic acid, maslinic acid and tormentic acid as active ingredients (Patent Document 4) ) And oleanolic acid have been reported to have anti-caries (prevention of dental caries) (Patent Document 5), anti-cancer action (Patent Document 6) and the like.
However, it has not been known that oleanane-type triterpenes have the effect of improving knee joint function improvement or skeletal muscle function enhancement by resistance training without using a training device.

International Publication 2002/043736 JP2014-141444 International Publication 2003/011267 JP 2006-347967 A JP-A-1-290619 JP-A-8-111986

Zhang W et al., Osteoarthritis Cartilage. 2008 16: 137-162 Juan ME et al., J Nutr., 136 (10), 2553-2557, 2006 Hashimoto H et al., J Orthop Sci (2003) 8: 288-293

  This invention makes it a subject to provide the composition for accelerating | stimulating the knee joint function improvement effect by resistance training which does not use a training apparatus. Another object of the present invention is to provide a composition for promoting skeletal muscle function enhancing action by resistance training without using a training device.

The present inventors promoted the knee joint function improving action when oleanane-type triterpene is ingested compared to the non-ingestion group when the subject is performing resistance training without using a training device, and skeletal muscle It was found that the function enhancement action was promoted.
That is, the present invention is as follows.
[1] A composition containing oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient for promoting the knee joint function improving effect by resistance training without using a training device,
[2] A composition for promoting enhancement of skeletal muscle function by resistance training without using a training device, comprising oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient,
[3] The composition according to [2] above, wherein the enhancement of skeletal muscle function includes enhancing muscle strength and / or increasing muscle mass,
[4] The composition according to any one of [1] to [3], wherein the oleanane-type triterpene is selected from maslinic acid and oleanolic acid or a mixture thereof,
[5] The composition according to [4], wherein the resistance training without using a training device is one or a combination of two or more selected from the group consisting of self-weight exercise, tube exercise, martial arts, or type exercises of body arts.
[6] The composition according to claim 5, wherein the type movement of martial arts or body surgery is tai chi.
[7] A food containing an oleanane-type triterpene or a pharmacologically acceptable salt thereof as an active ingredient, and a label indicating that the action of improving knee joint function by resistance training without using a training device is promoted, and [8 ] Food containing an oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient, and a label indicating that enhancement of skeletal muscle function is promoted by resistance training without using a training device.

  The composition containing the oleanane type triterpene of the present invention as an active ingredient can promote the knee joint function improving action and the skeletal muscle function enhancing action during resistance training without using a training device, and can be safely used in arthritic patients and the like. Function can be improved.

It is a HPLC analysis chart of the liquid containing triterpenes obtained from olive fruit. The peak of A is maslinic acid, and the peak of B is oleanolic acid. The peak was identified by comparing the retention time with a standard product and by LC / MS analysis. The quantitative value was calculated based on the peak area of the standard product.

  In the present invention, resistance training is an isometric exercise and / or an isometric exercise that applies a load (resistance) to local or whole body muscle groups to improve skeletal muscle functions such as muscle strength, muscle power, and muscle endurance. Say. Here, the isometric exercise is a static muscular strength strengthening method characterized by applying a certain load to the muscle without moving the joint, and contracting without changing the length of the muscle. Isotonic exercise is a dynamic muscle strength strengthening method characterized by moving a joint, applying a certain load to the muscle, and contracting while changing the length of the muscle.

  Resistance training is roughly classified into a mode using training equipment such as a training bench, weight machine, exercise bike, walking machine, rowing machine, and vibration machine, and a mode not using such training equipment. The former is a facility-use type such as a training gym or a medical institution, and the latter is a facility-unnecessary type that does not choose a place, and has the advantage that it can be easily performed at home or in a park without cost.

  In the present invention, resistance training that does not use a training device is not particularly limited as long as it is resistance training in a mode that does not use a training device. For example, self-weight exercise, type exercise such as martial arts or body exercise, underwater exercise, dumbbell, wrist Resistance training using equipment such as weights, ankle weights, training tubes, balance balls, and jump ropes.

  Here, the self-weight exercise is an isometric exercise and / or an isometric exercise characterized by using the weight of the body as a load without using a training device or equipment.

  Underwater exercise is an isometric exercise characterized by lightening the burden on the legs and legs due to buoyancy and utilizing the resistance of water as a load.

  The martial arts and body type exercises such as tai chi performed for health purpose are said to be an aspect of bodywork like yoga and pilates, etc., but the slow movement keeps muscle tension for a long time. Therefore, it can be categorized into a resistance training weight system. In the present invention, it is preferable that the type movements such as martial arts and body surgery are tai chi from the viewpoint that the burden on the joint is small.

  In the present invention, resistance training without using a training device can be performed by combining one or more, and preferably one or more selected from the group consisting of self-weight exercise, tube exercise, martial arts, or type exercise of body art It is a combination.

  As for the self-weight exercise, (1) About the triceps surae, lie down on your face, place your hands on the floor at your shoulders, bend your elbows, stretch your arms with your knees straight, straighten from your knees to your head, and slowly return Exercise (2) For the thigh muscle group, open the legs about the shoulder width, open the toes at about 45 degrees, and bend the legs so that the knees sit while spreading in the direction of the toes (make sure the knees do not come out before the toes) , Exercise so that the waist does not exceed 90 degrees), (3) For the large ankle muscles, sleep on your back, open your legs lightly, stand your knees, and straighten from knee to chest (4) For the back muscles, lie down on your back, place your hands on the floor at the chest position, lift your left hand forward and stretch it, lift your right leg straight, and slowly return your legs (alternating legs and legs) To repeat), (5 With regard to the muscles around the knee, sleep on the back, support with the elbows, raise the upper body, raise both knees, lift one leg straight, extend the knees straightly (repeat both legs alternately), (6) body side For muscle groups, lie down on the arm pillow, stand up the pelvis vertically with respect to the floor, raise the legs with the knees stretched, and slowly move back (both legs), (7) supine on the abdominal muscles Sleep, open your legs lightly, put your knees up, place your hands next to your body, raise your upper body as if you look at the chin, and slowly return it. (8) Sleep on your back and support with your elbows Examples include, but are not limited to, raising the upper body, bending the knees, bringing the thighs closer to the chest, and slowly returning them (replacing both feet alternately).

  For tube movement, for example, rubber tube ("Ceraband tube" manufactured by D & M Co., Ltd.) is used. (1) Grasp both ends of the tube with each hand and pull one hand down from a state where both hands are raised. (2) Open the arm up and down in front of the chest, and grasp both ends of the tube with each hand. Pull up one arm at a time so as not to bend the elbow and slowly return it. (3) Stand with your foot open slightly wider than your shoulder width, and stop the end of the tube with one foot. Hold the end of the band with one hand and pull it up until it is level with your shoulders with your elbows extended and slowly return. (4) Stand with both feet aligned, step on the looped band with both feet, and hold the tube slightly forward with the spine stretched. Pull up with your arms stretched and slowly return. (5) Stand with your legs wide open, grasp the left and right sides of the looped tube with both hands, and extend your arms straight forward. Spread your arms in front of your chest without bending your elbows and slowly return. (6) Stand with your feet open to the shoulder width, hold the looped tube with one foot, place the arm on the same side on the body side and grab the band. Pull up backwards slowly so that the elbow is not bent. (7) Hold the middle of the tube with both feet aligned and hold both ends with their hands. Pull it up with just the shoulder force, and slowly return it. (8) Stand with your legs open to the shoulder width, hold the looped band with one leg, and grab the tube on your body side. Bend the elbow and pull it up and slowly return it. (9) Extend both arms straight forward and hold both ends of a short tube. The wrist can be turned outward and returned slowly, but is not limited thereto.

  Tai Chi is preferred as a martial art or body type exercise, and examples of Tai Chi include, but are not limited to, Neng Gong 18 method, 24 formula, 88 formula, and the like. Neriko 18 method (first stage) for the purpose of mild stretch is preferred.

  The period, frequency, and configuration of the resistance training without using the training device are not particularly limited as long as the effects of the knee joint function improving action and the skeletal muscle function enhancing action by the training can be obtained. For example, it can be performed 1 to 7 times a week, preferably 4 to 7 weeks, over a period of 4 to 50 weeks, preferably 8 to 30 weeks. In addition, it can be performed by a program consisting of three categories: preparatory exercise including stretching, recreation and games, main exercise which is resistance training without using training equipment, and organizing exercise including static stretching for the purpose of preparing the whole body. .

The composition of the present invention contains oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.
In the present invention, a triterpene is a compound having a basic skeleton having 30 carbon atoms, and a typical example thereof is a pentacyclic triterpene. Here, the pentacyclic triterpene is a kind of triterpenes, and is a pentacyclic compound composed of 6 isoprene units, which basically has 30 carbon atoms, but is rearranged, oxidized, Also included are those that are desorbed or alkylated and have about the number of carbon atoms.
These can be obtained from natural plants or artificially. Moreover, a commercial item can also be utilized suitably. Pentacyclic triterpenes are generally classified according to their skeleton. Examples include oleanane type triterpenes, ursan type triterpenes, lupine type triterpenes, hopane type triterpenes, seratane type triterpenes, freederan type triterpenes, taraxelan type triterpenes, taraxastane type triterpenes, multiflorane type triterpenes, germanic type triterpenes and the like.
Among pentacyclic triterpenes, for example, representative examples of oleanane-type triterpenes include maslinic acid and oleanolic acid.

  Maslinic acid is contained in olive fruits and leaves, jujube, almonds, banaba leaves, sage, apples, cranberries, karin and the like. Oleanolic acid is olive fruit and leaves, grape, beet, jujube, almond, banaba leaf, sage, hawthorn, raspberry, karin, rosemary leaf, guava, perilla leaf, blueberry, prune, loquat, pomegranate, lemon balm, basil, rose Included in hips, oysters, assemblies, etc. Ursolic acid is found in olive leaves, almonds, apples, cranberries, hawthorn, raspberries, quince, rosemary leaves, guava, perilla leaves, blueberries, prunes, loquat, pomegranates, lemon balm, basil, rose hips, oysters, sea bream, bearberry It is.

  In the present invention, the oleanane-type triterpene is preferably selected from maslinic acid, oleanolic acid, and mixtures thereof.

These oleanane-type triterpenes can be obtained by extraction and purification from the above-mentioned plants by a known method, and can also be obtained by chemical synthesis. The oleanane triterpene may be a single component or a mixture of several components. Moreover, it can also be used in the form of a pharmacologically acceptable salt and / or derivative.
The oleanane-type triterpene may be used in the form of the plant extract. It is particularly preferable to use the form of an olive fruit extract because it has a long experience of food, is rich in oleanane-type triterpenes, and can be used after oil extraction.

As a method for producing oleanane type triterpenes, for example, dried, semi-dried or undried olive fruits, olive oil milled rice produced in the oil extraction process, starting materials, lower alcohols (ethanol, Methanol, n-propanol, isopropanol, n-butanol, etc.), or a method of extracting triterpene with a water-containing alcohol thereof. If necessary, saponification treatment and neutralization are performed, and octadecyl silica (ODS), silica gel, synthetic adsorbent, etc. are used as the adsorbent, and fractionated and purified to contain maslinic acid and / or oleanolic acid as the main components. You may obtain the mixture of the triterpene containing as.
When other plants are used as starting materials, oleanane-type triterpenes can be obtained by a method suitable for the plant used as the raw material. Moreover, the kind and component ratio of the component of the oleanane type | mold triterpene obtained depend on the plant used as a raw material.

The intake amount of oleanane-type triterpenes varies depending on the age, sex, symptom level, and intake form of patients with arthropathy to be ingested. Is 1.0 mg maslinic acid equivalent / kg or more. There is no particular upper limit on the amount of intake, but if it exceeds 60 mg maslinic acid equivalent / kg, it is not preferable from the viewpoint of product cost.
The maslinic acid equivalent represents the amount of a mixture of maslinic acid and a compound other than maslinic acid by the mass of maslinic acid contained in the mixture. Specifically, 1.0 mg maslinic acid equivalent of olive fruit-derived triterpene is a mixture of oleanolic acid and other peak compounds other than maslinic acid shown in the chart of FIG. Means the amount.

  Oleanan type triterpenes are divided into 1 to 3 times during the period of resistance training without using training equipment, preferably 4 to 50 weeks, more preferably 8 to 30 weeks according to the period of resistance training. Ingest. During the resistance training period, it is preferable to take every day regardless of the frequency of the resistance training, that is, whether the resistance training is performed or not. As for the timing of ingestion, when it is a day with resistance training, it does not matter before and after the resistance training performed on that day.

  The composition of the present invention can be formulated into an arbitrary form together with an auxiliary agent into a pharmaceutical product that can be administered orally or parenterally. For example, the oral dosage form can be a solid dosage form such as a tablet, an orally disintegrating tablet, a capsule, a granule or a fine granule, or a liquid dosage form such as a syrup or suspension. The parenteral dosage form is administered in the form of injections, eye drops, nasal drops, patches, suppositories, and external preparations for skin. Pharmaceutical products include quasi-drugs.

  In the case of a solid dosage form, it may contain an appropriate amount of various additives used for the production of general preparations. Examples of such additives include excipients, binders, acidulants, foaming agents, artificial sweeteners, fragrances, lubricants, colorants, stabilizers, pH adjusters, and surfactants.

  In the case of a liquid dosage form, the composition of the present invention is prepared by a conventional method in the presence of a pH adjusting agent, a buffering agent, a solubilizing agent, a suspending agent, etc., an isotonic agent, a stabilizing agent, a preservative and the like. Can be formulated.

  The form of the external preparation for skin is not particularly limited. For example, cosmetics such as emulsions, creams, lotions, packs, dispersions, cleaning agents, makeup cosmetics, scalp / hair products, ointments, creams, and external use It can be a pharmaceutical such as a liquid. In addition to the above components, components commonly used in skin external preparations such as cosmetics and pharmaceuticals, such as whitening agents, moisturizers, various skin nutritional components, ultraviolet absorbers, antioxidants, oily components, surfactants, thickeners , Alcohols, coloring agents, water, preservatives, fragrances and the like can be appropriately blended as necessary.

  The composition of the present invention is prepared as a functional food, health food, food for specified health use, health function food such as functional nutrition food, special use food (for example, food for patients), health supplement, supplement, etc. Also good. As supplements, for example, tablets, rounds, capsules (including hard capsules, soft capsules, microcapsules), powders, granules, fine granules, troches, jelly, together with various additives used in the production of general supplements And liquid (including syrup, milk, and suspension).

  The composition of this invention can be mix | blended with a foodstuff. There are no particular limitations on the foods that can be blended, for example, confectionery such as candy, gummi, chewing gum, etc .; confectionery such as cookies, crackers, biscuits, chocolate, pudding, jelly, snacks, rice confectionery, buns, and sheep candy; ice cream , Ice candy, sorbet, gelato and other frozen desserts; bakery foods such as donuts, cakes, bread, French bread, croissants; noodles such as udon, buckwheat, Chinese noodles, kishimen, etc .; cooked rice such as white rice, red rice, pilaf; curry, stew Sauces such as dressings; kneaded products such as ham, sausage, kamaboko, chikuwa, fish sausage; various side dishes such as tempura, croquette, hamburger; beverages such as juice and tea.

  EXAMPLES Examples will be shown below for specifically explaining the present invention, but the present invention is not limited to the following examples.

Production Example 1 [Method for producing olive fruit-derived oleanane-type triterpene]
An olive fruit oil lees from which olive oil was removed by a known oil extraction method such as pressing was obtained. After adding 250 g of 90% aqueous ethanol (v / v) to 100 g of this olive fruit oil cake and heating at 70 ° C. with stirring for 2 hours, the liquid portion obtained by solid-liquid separation was distilled off under reduced pressure using an evaporator or the like. A concentrated solution was obtained. This concentrated solution was dispersed in water, subjected to column chromatography using synthetic adsorption resin Amberlite XAD4 (manufactured by Organo) as a carrier, and increased by 10% stepwise elution from 50% aqueous ethanol to 100% ethanol. A content fraction was obtained and distilled under reduced pressure to obtain a liquid olive fruit-derived oleanane-type triterpene. This triterpene-containing liquid contained maslinic acid and oleanolic acid in a ratio of 10: 4 (FIG. 1). The peak was identified by comparing the retention time with a standard product and by LC / MS analysis. The quantitative value was calculated based on the peak area of the standard product.
Γ-Cyclodextrin was added and mixed so that the maslinic acid content was 10% by mass, dispersed in water, and then spray-dried to obtain a powdered olive fruit-derived oleanane-type triterpene.

Production Example 2 [Production of triterpene-containing supplement]
The raw materials were mixed with the formulation shown in Table 1 to prepare stick jelly. The content per stick jelly is 10 g, and the contents of maslinic acid and oleanolic acid are 30 mg and 12 mg, respectively.

Table 1 Composition of stick jelly

Test Example 1 [Evaluation of the effect on resistance training without using training equipment in patients with arthropathy]
(1) Criteria for clinical trials and criteria for determination of effects / subject selection (selection criteria)
1) Japanese
2) Over 65 years old
3) We do not receive care certification such as care required
4) Exercise for more than a year, such as stretching
5) A person who has received sufficient explanation about the purpose and content of the study, has the ability to consent, and who volunteered to participate in the study voluntarily after understanding it, who has agreed to participate in the study in writing.
6) A person who can observe the handling of the test article in the prescribed manner
7) Person who can follow the person concerned with the test

(Exclusion criteria)
1) A person who has a disease (heart disease, liver disease, diabetes, etc.) prohibited from exercise by a doctor 2) A person who has been diagnosed with dementia from a doctor / A person who is taking a dementia treatment
3) Those who are allergic to the raw material of the olive fruit extract to be ingested
4) Those who were judged to be inappropriate for the research team to participate in the study Based on the above criteria, subjects were selected from 70-76 year old arthropathy patients etc. who attend the Department of Health Promotion at Senior Colleges.
In carrying out resistance training without using a training device, it was randomly divided into two groups, a non-intake group (comparative example) and an ingestion group (example) of supplements containing oleanane-type triterpenes. There were 20 non-ingestion groups (6 men and 14 women), of whom 1 woman withdrew from training due to poor physical condition. The intake group was 19 people (6 men and 13 women), of whom 2 women left training due to poor physical condition. In addition, the poor physical condition of 3 persons was not specified to be caused by this test.
(2) Intake method of oleanane-type triterpene-containing supplements In the oleanane-type triterpene-containing supplement intake group, two stick jelly of the examples described in Table 1 once a day after breakfast (60 mg / day as maslinic acid), 12 Ingested daily for a week. In the non-ingestion group, the stick jelly of Table 1 Comparative Example was ingested in the same manner.

(3) Resistance training without using training equipment Tai Chi, tube exercise, and self-weight exercise were adopted. The resistance training period for tai chi, tube exercise, and self-weight exercise was 12 weeks, and was attended once a week. The program consists of the three categories of preparation exercise shown in Table 2, main exercise which is resistance training, and organizing exercise.

＊１：ゴム製チューブ（「セラバンドチューブ」D＆M 株式会社デイエム商会製）を使用した Table 2 Training program

* 1: A rubber tube ("Ceraband tube" D & M, manufactured by DIM Co., Ltd.) was used.

(4) Test result 1: Effect of oleanane type triterpene on body composition after resistance training without using training equipment 1) Body composition Body fat percentage and muscle mass were trained using InBody S10 (manufactured by Inbody) Table 3 shows measured values before and after resistance training without using the equipment. The body fat percentage after resistance training hardly changed in the comparative example, but a significant decrease (p <0.05) was observed in the examples. The total body skeletal muscle mass after resistance training was slightly decreased in the comparative example, but a significant increase (p <0.1) was observed in the examples. When the skeletal muscle mass was measured by site, a significant increase (p <0.01) was observed in the right arm, left arm, and trunk muscle of the Examples, and these increases were tested between groups with the comparative example. (P <0.05).

Table 3

(5) Test result 2: Effect of oleanane-type triterpene on muscle strength after resistance training without using training equipment 2) Muscle strength As a representative example of muscle strength, grip strength of the dominant arm was measured using TKK5101 GRIP-D (manufactured by Takei Equipment). did. Table 4 shows the measured values before and after resistance training without using the training equipment. The grip strength after resistance training tended to be strong in the comparative example, but there was no significant difference, and the grip strength was significantly strong in the example (p <0.05).

Table 4

(6) Test result 3: Effect of oleanan type triterpene on knee physical function after resistance training without using training equipment Knee body of “WOMAC version LK3.0 for the Japanese scale” (see Non-Patent Document 3) shown below A questionnaire was conducted on 17 questions related to function and 5 questions related to knee pain for the right and left knees. Total score for knee physical function, total score for knee pain for right knee, and knee pain for left knee The total score was summed up and the total score was taken as the total score. The results are shown in Table 5.

(Question about knee physical function)
Looking back over the past two weeks, please tell us how difficult the following daily operations were due to knee symptoms.
Evaluation item 1. Go down the stairs.
2. Go up the stairs.
3. Get up from the chair.
4). Is standing.
5). Bend over to the floor.
6). Walk on the flat ground.
7). Get on and off the passenger car.
8). Go shopping.
9. Put on socks.
10. Get up from the bed.
11. Take off socks.
12 Lying on the bed.
13. Enter and exit the bathroom.
14 Sitting in a chair.
15. Use a Western-style toilet.
16. Clear up heavy objects.
17. Do housework such as cooking and washing.

(Questions about knee pain)
Looking back over the past two weeks, how much do you feel your knees hurt when you do the following?
Evaluation item 1. How much pain did you feel when walking on flat ground?
2. How painful did you feel when going up and down the stairs?
3. How much pain did you feel when lying on the bed at night?
4). How much pain did you feel when sitting on a chair or lying on the floor?
5). How much pain did you feel when standing upright?

比較例では、レジスタンストレーニング前後で有意に改善した項目はなく、左膝痛において改善傾向にあった。それに対して実施例では、レジスタンストレーニング前後で全体的に改善傾向にあり、特に右膝痛では有意に改善し（ｐ＜０．０５）、比較例との群間比較でも優位な改善（ｐ＜０．０５）であった。 Table 5

In the comparative example, there was no item that significantly improved before and after resistance training, and there was a tendency to improve left knee pain. On the other hand, in the examples, there was an overall improvement trend before and after resistance training, particularly significant improvement in right knee pain (p <0.05), and a significant improvement (p <0.05) in comparison with the comparative example. 0.05).

Claims (8)

  A composition for promoting knee joint function improving action by resistance training without using a training device, comprising oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.   A composition for promoting skeletal muscle function enhancement by resistance training without using a training device, comprising oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.   The composition of claim 2, wherein augmenting skeletal muscle function comprises enhancing muscle strength and / or increasing muscle mass.   The composition according to any one of claims 1 to 3, wherein the oleanane-type triterpene is selected from maslinic acid and oleanolic acid or a mixture thereof.   The composition according to claim 4, wherein the resistance training without using a training device is one or a combination of two or more selected from the group consisting of self-weight exercise, tube exercise, martial art, or type exercise of body art.   The composition according to claim 5, wherein the martial art or body art type movement is Tai Chi.   A food that contains oleanane-type triterpene or a pharmacologically acceptable salt thereof as an active ingredient, and is labeled as promoting the improvement of knee joint function by resistance training without using a training device.   A food comprising an oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient, and a label indicating that the enhancement of skeletal muscle function by resistance training without using a training device is promoted.

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