JP2017109941A - Composition for promoting knee joint function improvement action and skeletal muscle function enhancement action by resistance training, comprising oleanane-type triterpene as active ingredient - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for promoting a knee joint function improvement action by resistance training and a composition for promoting a skeletal muscle function enhancement action by resistance training.SOLUTION: The problems is solved by employing oleanane-type triterpene as an active ingredient.SELECTED DRAWING: None

Description

  The present invention relates to a composition for promoting the knee joint function improving action by resistance training containing oleanane type triterpene as an active ingredient. The present invention also relates to a composition for promoting skeletal muscle function enhancing action by resistance training containing oleanane type triterpene as an active ingredient.

Vertebrates including humans are at risk of developing motor organ syndrome (also known as locomotive syndrome) due to aging and lifestyle habits. The musculoskeletal syndrome was defined by the Japanese Society of Clinical Orthopedic Surgery as “a state in which the risk of needing long-term care due to musculoskeletal decline or disability” increases. There are many cases of musculoskeletal syndrome, such as traumatic arthropathy due to traumatic causes such as injury, rheumatoid arthritis which is a kind of autoimmune disease, degenerative spondylosis due to degeneration or deformation of intervertebral disc, muscle with aging These include reduction, neuropathy due to stenosis, bone abnormalities due to fractures and osteoporosis, osteoarthritis, and the like.
Almost all joints of vertebrates have cartilage, which is important for providing smooth joint movement such as joint flexibility and compressibility. Many symptoms are caused, including decreased sex, inflammation of joints and surrounding tissues, and pain. Examples of such symptoms include osteoarthritis. Osteoarthritis tends to develop in weight-bearing joints such as knees, hips and ankles. Although traumatic joint damage can be the cause, most are due to cartilage wear and degeneration and aging with age.
The International Society for Arthritis recommends resistance training as a coping therapy for people with knee pain (Non-patent Document 1). Resistance training is an isometric exercise and / or an isotonic exercise that applies a load (resistance) to local or whole body muscle groups to improve skeletal muscle functions such as muscle strength, muscle power, and muscle endurance. Here, the isometric exercise is a static muscular strength strengthening method characterized by applying a certain load to the muscle without moving the joint, and contracting without changing the length of the muscle. Isotonic exercise is a dynamic muscle strength strengthening method characterized by moving a joint, applying a certain load to the muscle, and contracting while changing the length of the muscle.
Resistance training is broadly divided into a mode using a training device and a mode not using such a training device. The former is a facility use type such as a training gym or a medical institution, and the latter is a facility unnecessary type that does not choose a place.
For an arthritic patient or an injured person or a potential injured person (hereinafter referred to as an arthritic patient) having knee pain, mild load resistance training that can be performed without pain as much as possible is preferable. As a result, when the muscles around the joint are strengthened and the force to support the joint is increased, the sense of stability of the joint is increased, so the burden on the joint is reduced, and as a result, the pain of the joint can be alleviated.
In recent years, whole body vibration training has attracted attention as a new resistance training method. Whole body vibration training uses a device that vibrates at high speed as a training device, and passively and continuously causes muscle contraction due to high-speed vibration when performing isometric or isotonic exercise with a light load. Training (this phenomenon is called tension vibration reflex muscle contraction). In general resistance training, it is necessary to apply a load and actively cause muscle contraction, which often causes pain for arthritis patients and the like. Whole body vibration training is a passive arterial contraction and is therefore a good treatment for arthropathy that is painless and greatly reduced.
So far, reports on the effects of whole body vibration training for patients with knee osteoarthritis have been seen mainly in Europe and the United States (Non-patent Document 2). In addition, it has been shown that the practice of whole body vibration training in Japanese middle-aged and elderly women with knee osteoarthritis and knee pain may be effective in improving knee function and physical function mainly in the lower limbs (Non-Patent Documents 3 and 4).

Triterpenes are a group of compounds widely distributed in the plant kingdom, and are a group of compounds classified into oleanane type triterpenes and ursan type triterpenes. The oleanane type triterpenes include maslinic acid, oleanolic acid, and the like, and the ursan type triterpenes include ursolic acid, corosolic acid, tormic acid, and the like.
Plant-derived oleanane-type triterpenes have recently attracted attention as functional components having physiological activity. Maslinic acid inhibits colon cancer (non-patent document 5), skin whitening (patent document 1), and osteoclast differentiation inhibition. And bone resorption activity effect (Patent Document 2), obesity prevention effect (Patent Document 3), composition containing indigestible dextrin, corosolic acid, maslinic acid and tormentic acid as active ingredients (Patent Document 4) ) And oleanolic acid have been reported to have anti-caries (prevention of dental caries) (Patent Document 5), anti-cancer action (Patent Document 6) and the like.
However, it has not been known that oleanane-type triterpenes have an effect of improving knee joint function improvement or skeletal muscle function enhancement by resistance training.

International Publication 2002/043736 JP2014-141444 International Publication 2003/011267 JP 2006-347967 A JP-A-1-290619 JP-A-8-111986

Zhang W et al., Osteoarthritis Cartilage. 2008 16: 137-162 Simao AP et al., Arch Phys Med Rehabil. 2012 93: 1692-1700 Avelar NC et al., J Altern Complement Med. 2011 17: 1149-1155 Jieun Yoon et al., Physical Fitness. 2014 Volume 63 Issue 4: 371-382 Juan ME et al., J Nutr., 136 (10), 2553-2557, 2006

  An object of the present invention is to provide a composition for promoting the knee joint function improving action by resistance training. Another object of the present invention is to provide a composition for promoting the skeletal muscle function enhancing action by resistance training.

The present inventors, when the subject is taking resistance training, ingesting oleanane-type triterpene, promotes the knee joint function improvement action compared to the non-intake group, and also enhances the skeletal muscle function enhancement action Found to promote.
That is, the present invention is as follows.
[1] A composition for promoting knee joint function improving action by resistance training, comprising oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient,
[2] A composition for promoting enhancement of skeletal muscle function by resistance training, comprising oleanane-type triterpene or a pharmacologically acceptable salt thereof as an active ingredient,
[3] The composition according to [1] or [2], wherein the resistance training is resistance training using a training device,
[4] The composition according to any one of [1] to [4], wherein the training device is a high-speed vibration device, and the composition [5] skeletal muscle is lower limb muscle.
[6] The composition according to any one of [1] to [5], wherein the oleanane-type triterpene is selected from maslinic acid and oleanolic acid or a mixture thereof.

  The composition containing the oleanane type triterpene of the present invention as an active ingredient can promote the knee joint function improving action and the skeletal muscle function enhancing action during resistance training, and can improve the body function safely even in arthropathy patients etc. I can do it.

It is a HPLC analysis chart of the liquid containing triterpenes obtained from olive fruit. The peak of A is maslinic acid, and the peak of B is oleanolic acid. The peak was identified by comparing the retention time with a standard product and by LC / MS analysis. The quantitative value was calculated based on the peak area of the standard product.

  In the present invention, resistance training is an isometric exercise and / or an isometric exercise that applies a load (resistance) to local or whole body muscle groups to improve skeletal muscle functions such as muscle strength, muscle power, and muscle endurance. Say. Here, the isometric exercise is a static muscular strength strengthening method characterized by applying a certain load to the muscle without moving the joint, and contracting without changing the length of the muscle. Isotonic exercise is a dynamic muscle strength strengthening method characterized by moving a joint, applying a certain load to the muscle, and contracting while changing the length of the muscle.

  Resistance training is roughly classified into a mode using training equipment such as a training bench, weight machine, exercise bike, walking machine, rowing machine, and vibration machine, and a mode not using such training equipment. The former is a facility-use type such as a training gym or a medical institution, and the latter is a facility-unnecessary type that does not choose a place.

  As resistance training in a mode that does not use training equipment, for example, weight training, type exercises such as martial arts and body surgery, underwater exercise, and equipment such as dumbbells, wrist weights, ankle weights, training tubes, balance balls, and jump ropes were used. Resistance training.

  In the present invention, the type of resistance training is not particularly limited, but from the viewpoint of less burden on the knee joint, self-weight exercise, underwater exercise, type exercise such as martial arts and body surgery, and whole body vibration training are preferable, and whole body vibration training is most preferable. It is.

  Here, the self-weight exercise is an isometric exercise and / or an isometric exercise characterized by using the weight of the body as a load without using a training device or equipment.

  Underwater exercise is an isometric exercise characterized by lightening the burden on the legs and legs due to buoyancy and utilizing the resistance of water as a load.

  The martial arts and body type exercises such as tai chi performed for health purpose are said to be an aspect of bodywork like yoga and pilates, etc., but the slow movement keeps muscle tension for a long time. Therefore, it can be categorized into a resistance training weight system.

  Whole body vibration training is a training that passively and continuously causes muscle contraction due to high-speed vibration when performing isometric exercise and / or isotonic exercise using a device that vibrates at high speed as a training device. Although the high-speed vibration apparatus used for the whole body vibration training is not particularly limited, for example, PowerPlate Pro5 (Power Plate International LTD, London, UK) can be used.

  The whole body vibration training program is not particularly limited, and is composed of, for example, three categories of warm-up, resistance, and cool-down. The warm-up is preferably composed of several postures (stretching muscles) with muscles and tendons stretched. The resistance is preferably composed of several postures (isometric exercise and / or isotonic exercise) that give a load to the body, and it is preferable to sequentially shift from a posture with a small load to a posture with a large load. . It is preferable that the cool-down is composed of several postures (muscle relaxation) that remove power from the whole body.

  In the whole body vibration training, the vibration frequency, the vibration amplitude, and the vibration time for one kind of posture can be appropriately changed, but the vibration frequency is preferably 25 to 50 Hz, more preferably 30 to 40 Hz. preferable. The amplitude of vibration is preferably 1 to 10 mm, and more preferably 2.5 to 4 mm. In addition, for one kind of posture, the vibration time is preferably 30 to 540 seconds, and more preferably 30 to 60 seconds.

  The period, frequency, and configuration of resistance training are not particularly limited as long as the effect of improving knee joint function and skeletal muscle function is enhanced by training, but for example, 4 to 50 weeks, preferably 10 to 30 weeks. Can be carried out at a frequency of 1 to 4 times a week, preferably 2 to 3 times a week over a period of.

The composition of the present invention contains oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.
In the present invention, a triterpene is a compound having a basic skeleton having 30 carbon atoms, and a typical example thereof is a pentacyclic triterpene. Here, the pentacyclic triterpene is a kind of triterpenes, and is a pentacyclic compound composed of 6 isoprene units, which basically has 30 carbon atoms, but is rearranged, oxidized, Also included are those that are desorbed or alkylated and have about the number of carbon atoms.
These can be obtained from natural plants or artificially. Moreover, a commercial item can also be utilized suitably. Pentacyclic triterpenes are generally classified according to their skeleton. Examples include oleanane type triterpenes, ursan type triterpenes, lupine type triterpenes, hopane type triterpenes, seratane type triterpenes, freederan type triterpenes, taraxelan type triterpenes, taraxastane type triterpenes, multiflorane type triterpenes, germanic type triterpenes and the like.
Among pentacyclic triterpenes, for example, representative examples of oleanane-type triterpenes include maslinic acid and oleanolic acid.

  Maslinic acid is contained in olive fruits and leaves, jujube, almonds, banaba leaves, sage, apples, cranberries, karin and the like. Oleanolic acid is olive fruit and leaves, grape, beet, jujube, almond, banaba leaf, sage, hawthorn, raspberry, karin, rosemary leaf, guava, perilla leaf, blueberry, prune, loquat, pomegranate, lemon balm, basil, rose Included in hips, oysters, assemblies, etc. Ursolic acid is found in olive leaves, almonds, apples, cranberries, hawthorn, raspberries, quince, rosemary leaves, guava, perilla leaves, blueberries, prunes, loquat, pomegranates, lemon balm, basil, rose hips, oysters, sea bream, bearberry It is.

  In the present invention, the oleanane-type triterpene is preferably selected from maslinic acid, oleanolic acid, and mixtures thereof.

These oleanane-type triterpenes can be obtained by extraction and purification from the above-mentioned plants by a known method, and can also be obtained by chemical synthesis. The oleanane triterpene may be a single component or a mixture of several components. Moreover, it can also be used in the form of a pharmacologically acceptable salt and / or derivative.
The oleanane-type triterpene may be used in the form of the plant extract. It is particularly preferable to use the form of an olive fruit extract because it has a long experience of food, is rich in oleanane-type triterpenes, and can be used after oil extraction.

As a method for producing oleanane type triterpenes, for example, dried, semi-dried or undried olive fruits, olive oil milled rice produced in the oil extraction process, starting materials, lower alcohols (ethanol, Methanol, n-propanol, isopropanol, n-butanol, etc.), or a method of extracting triterpene with a water-containing alcohol thereof. If necessary, saponification treatment and neutralization are performed, and octadecyl silica (ODS), silica gel, synthetic adsorbent, etc. are used as the adsorbent, and fractionated and purified to contain maslinic acid and / or oleanolic acid as the main components. You may obtain the mixture of the triterpene containing as.
When other plants are used as starting materials, oleanane-type triterpenes can be obtained by a method suitable for the plant used as the raw material. Moreover, the kind and component ratio of the component of the oleanane type | mold triterpene obtained depend on the plant used as a raw material.

The intake amount of oleanane-type triterpenes varies depending on the age, sex, symptom level, and intake form of patients with arthropathy to be ingested. Is 1.0 mg maslinic acid equivalent / kg or more. There is no particular upper limit on the amount of intake, but if it exceeds 60 mg maslinic acid equivalent / kg, it is not preferable from the viewpoint of product cost.
The maslinic acid equivalent represents the amount of a mixture of maslinic acid and a compound other than maslinic acid by the mass of maslinic acid contained in the mixture. Specifically, 1.0 mg maslinic acid equivalent triterpene derived from olive fruit includes oleanolic acid containing 1.0 mg maslinic acid and other peak compounds other than maslinic acid shown in the chart of FIG. Means the amount of the mixture.

  The oleanane type triterpene is taken in 1 to 3 times during the period of resistance training, preferably 4 to 50 weeks, more preferably 10 to 30 weeks according to the period of resistance training. During the resistance training period, it is preferable to take every day regardless of the frequency of the resistance training, that is, whether the resistance training is performed or not. As for the timing of ingestion, when it is a day with resistance training, it does not matter before and after the resistance training performed on that day.

  The composition of the present invention can be formulated into an arbitrary form together with an auxiliary agent into a pharmaceutical product that can be administered orally or parenterally. For example, the oral dosage form can be a solid dosage form such as a tablet, an orally disintegrating tablet, a capsule, a granule or a fine granule, or a liquid dosage form such as a syrup or suspension. The parenteral dosage form is administered in the form of injections, eye drops, nasal drops, patches, suppositories, and external preparations for skin. Pharmaceutical products include quasi-drugs.

  In the case of a solid dosage form, it may contain an appropriate amount of various additives used for the production of general preparations. Examples of such additives include excipients, binders, acidulants, foaming agents, artificial sweeteners, fragrances, lubricants, colorants, stabilizers, pH adjusters, and surfactants.

  In the case of a liquid dosage form, the composition of the present invention is prepared by a conventional method in the presence of a pH adjusting agent, a buffering agent, a solubilizing agent, a suspending agent, etc., an isotonic agent, a stabilizing agent, a preservative and the like. Can be formulated.

  The form of the external preparation for skin is not particularly limited. For example, cosmetics such as emulsions, creams, lotions, packs, dispersions, cleaning agents, makeup cosmetics, scalp / hair products, ointments, creams, and external use It can be a pharmaceutical such as a liquid. In addition to the above components, components commonly used in skin external preparations such as cosmetics and pharmaceuticals, such as whitening agents, moisturizers, various skin nutritional components, ultraviolet absorbers, antioxidants, oily components, surfactants, thickeners , Alcohols, coloring agents, water, preservatives, fragrances and the like can be appropriately blended as necessary.

  The composition of the present invention is prepared as a functional food, health food, food for specified health use, health function food such as functional nutrition food, special use food (for example, food for patients), health supplement, supplement, etc. Also good. Examples of supplements include tablets, rounds, capsules (including hard capsules, soft capsules, and microcapsules), powders, granules, fine granules, troches, and liquids, as well as various additives used in the production of general supplements. (Including syrup, milk, and suspension).

  The composition of this invention can be mix | blended with a foodstuff. There are no particular limitations on the foods that can be blended, for example, confectionery such as candy, gummi, chewing gum, etc .; confectionery such as cookies, crackers, biscuits, chocolate, pudding, jelly, snacks, rice confectionery, buns, and sheep candy; ice cream , Ice candy, sorbet, gelato and other frozen desserts; bakery foods such as donuts, cakes, bread, French bread, croissants; noodles such as udon, buckwheat, Chinese noodles, kishimen, etc .; cooked rice such as white rice, red rice, pilaf; curry, stew Sauces such as dressings; kneaded products such as ham, sausage, kamaboko, chikuwa, fish sausage; various side dishes such as tempura, croquette, hamburger; beverages such as juice and tea.

  EXAMPLES Examples will be shown below for specifically explaining the present invention, but the present invention is not limited to the following examples.

Production Example 1 [Method for producing olive fruit-derived oleanane-type triterpene]
An olive fruit oil lees from which olive oil was removed by a known oil extraction method such as pressing was obtained. After adding 250 g of 90% aqueous ethanol (v / v) to 100 g of this olive fruit oil cake and heating at 70 ° C. with stirring for 2 hours, the liquid portion obtained by solid-liquid separation was distilled off under reduced pressure using an evaporator or the like. A concentrated solution was obtained. This concentrated solution was dispersed in water, subjected to column chromatography using synthetic adsorption resin Amberlite XAD4 (manufactured by Organo) as a carrier, and increased by 10% stepwise elution from 50% aqueous ethanol to 100% ethanol. A content fraction was obtained and distilled under reduced pressure to obtain a liquid olive fruit-derived oleanane-type triterpene. This triterpene-containing liquid contained maslinic acid and oleanolic acid in a ratio of 10: 4 (FIG. 1). The peak was identified by comparing the retention time with a standard product and by LC / MS analysis. The quantitative value was calculated based on the peak area of the standard product.
Γ-Cyclodextrin was added and mixed so that the maslinic acid content was 10% by weight, dispersed in water, and spray-dried to obtain a powdered olive fruit-derived oleanane-type triterpene.

Production Example 2 [Production of triterpene-containing supplement]
Raw materials were mixed with the formulation shown in Table 1, and filled into gelatin hard capsules so that the inner volume of the mixture was 250 mg. The maslinic acid content per capsule obtained in this test example is 17 mg, and the oleanolic acid content is 6.8 mg.

Test Example 1 [Evaluation of effects on whole body vibration training in patients with arthropathy]
(1) Clinical Trial Method and Criteria for Determination of Effect / Subject Selection The subjects were arthritic patients with pain in knees aged 65 to 80 who did not use a pacemaker and were not prohibited from exercising by doctors.
In carrying out resistance training, it was randomly divided into two groups: 20 non-intake group supplements containing oleanane-type triterpenes (comparative example) and 16 ingestion group (examples). The average age of the non-ingestion group was 71.1 years, with 5 men and 15 women. The average age of the intake group was 71.0 years old, with 5 men and 11 women. None of the subjects experienced retirement or physical abnormalities during resistance training.
(2) Oleanan-type triterpene-containing supplement intake method In the oleanane-type triterpene-containing supplement intake group, the capsules of the examples described in Table 1 were made once a day before breakfast (51 mg / day as maslinic acid), Ingested daily for 20 weeks. In the non-ingestion group, the capsules of Comparative Examples in Table 1 were ingested similarly.

(3) Resistance training Whole body vibration training was adopted in this test. Whole-body vibration training causes passive muscle contraction due to high-speed vibration, stimulating muscles to be equal to or better than performing active load training, even though the subject is in a static unloaded state can do.
Whole body vibration training was performed every Monday and Friday for a period of 20 weeks. The whole body vibration training program was composed of three categories of warm-up, resistance, and cool-down (Table 2), all of which were performed using PowerPlate Pro5 (Power Plate International LTD, London, UK). Warming up is composed of six postures (stretching muscles) with muscles and tendons stretched. The resistance is composed of six types of postures (isometric exercise or isometric exercise) that apply a load to the body, and sequentially shifts from R1 to R6 from a low-load posture to a high-load posture. The cool-down consists of four postures (muscle relaxation) that draws power from the whole body. The subject does not need to actively move in any posture except R4.
In the whole body vibration training, the vibration frequency and amplitude were 30 Hz and 2.5 mm, and the vibration time was 30 seconds for one kind of posture. In the warm-up and cool-down, W1 to W6 and C1 to C4 were implemented throughout the period. In resistance, R1 to R4 were performed in the 1st to 4th weeks, and R1 to R6 were performed in the 5th to 20th weeks.

^*注１：股関節屈曲、膝伸展、膝屈曲、股関節伸展の各姿勢を４秒間ずつ繰り返す等尺性運動と等張性運動の組合せ。

^* Note 1: Combination of isometric and isotonic exercises that repeats hip flexion, knee extension, knee flexion, and hip extension postures for 4 seconds each.

(4) Test result 1: Effect of oleanane-type triterpene on whole body vibration training by JOA score JOA score (Japanese Orthopaedic Association Score) is an objective and medical evaluation criteria for each joint function established by the Japanese Orthopedic Association It is. In the present invention, a knee joint JOA score (Table 3) was adopted, and knee joint function was evaluated before and after the start of whole body vibration training. It should be noted that the four areas of “pain / walking”, “pain / stepping ability”, “flexion angle and stiffness / high contracture (range of motion), and“ swelling ”are evaluated with 0-100 points, the higher the score. The function is good.

※腰野富久ほか：OA 膝治療成績判定基準．日整会誌,62：901〜902,1988

* Toshihisa Kumano et al .: OA knee treatment results criteria. Daily Journal, 62: 901-902,1988

The subjects who took the supplements of the comparative example and the example were evaluated for knee function by JOA score 7 days before the start of whole body vibration training and 14 days after the end. The results are shown in Table 4. Since the test subject had mild knee osteoarthritis, the scores for evaluation items 1, 3, and 4 before whole body vibration training were high and did not have a significant impact on normal life. There was a problem. After the whole body vibration training, in the non-intake group of the comparative example, the knee function tended to improve, and the improvement rate of the evaluation item 2 was 115%. In the intake group of the example, the knee function tended to have a higher improvement rate than the comparative example, and the improvement rate of the evaluation item 2 was particularly superior.
Of the 16 intake groups in the examples, 2 could not be measured due to non-physical reasons on the JOA score measurement date.

改善率 ＝ 全身振動トレーニング終了後の値／同開始前の値 × １００
Ｐ値は、全身振動トレーニング終了後／同開始前の優位差
関節数は、被験者の膝の痛みが両膝の場合には２、片膝の場合には１とカウントした。内訳は、比較例２０人の内５人が両膝、１５人が片膝であり、実施例１４人の内２人が両膝、１２人が片膝であった。

Improvement rate = value after completion of whole body vibration training / value before the start × 100
The P value was counted as 2 when the subject's knee pain was in both knees and 1 when the subject's knee pain was in one knee. The breakdown was that 5 out of 20 comparative examples were both knees, 15 were single knees, 2 of 14 examples were both knees, and 12 were single knees.

(5) Test result 2: Effect of oleanane type triterpene on whole body vibration training by lower limb muscle function evaluation Using a multipurpose muscle function evaluation exercise device (Biodex System 4, manufactured by Biodex), the knee joint was extended and bent. The muscle function was measured 7 days before the start of whole body vibration training and 14 days after the end.
The results are shown in Table 5. The oleanane-type triterpene intake group (Example) was significantly improved before and after the whole body vibration training in the evaluation items 1 to 3. The improvement rate of the evaluation items 1 to 3 of the intake group (Example) was superior to the improvement rate of the non-intake group (Comparative Example).

(Explanation of terms and evaluation items in Table 5)
Improvement rate: Value after end of whole body vibration training / Value before start x 100
P value: Predominant difference after completion of whole body vibration training / before the start: Number of joints was counted as 2 when the knee pain of the subject was both knees, and 1 when it was one knee. Breakdown was that 5 out of 20 comparative examples were both knees, 15 were single knees, 3 of 16 examples were both knees, and 13 were single knees.
Isokinetic knee movement: An isotonic movement in which the angular movement speed of the knee joint is controlled to a constant speed and the knee joint is repeatedly extended and bent. The muscle function of the knee can be evaluated by measuring the torque and power exerted during the isokinetic knee exercise. The evaluation items for isokinetic knee movement were measured by extending the knee from 90 degrees to 180 degrees (straightly stretched) at a speed of 60 degrees / second and bending movement in the opposite direction.

  From the above test results 1 and 2, it was shown that the composition containing the oleanane-type triterpene of the present invention as an active ingredient is effective for improving the knee joint function improvement and skeletal muscle function enhancement by resistance training. As for other safety, no bloody test, urine test, physical measurement and medical examination showed any medically problematic changes due to the ingestion of olive fruit extract.

Claims (6)

  A composition for promoting knee joint function improving action by resistance training, comprising oleanane-type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.   A composition for promoting enhancement of skeletal muscle function by resistance training, comprising oleanane type triterpene or a pharmacologically acceptable salt thereof as an active ingredient.   The composition according to claim 1 or 2, wherein the resistance training is resistance training using a training device.   4. The composition of claim 3, wherein the training equipment is a high speed vibration device.   The composition according to any one of claims 1 to 4, wherein the skeletal muscle is lower limb muscle.   The composition according to any one of claims 1 to 5, wherein the oleanane type triterpene is selected from maslinic acid and oleanolic acid or a mixture thereof.

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