JP2017060502A5 - - Google Patents
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- Publication number
- JP2017060502A5 JP2017060502A5 JP2016226816A JP2016226816A JP2017060502A5 JP 2017060502 A5 JP2017060502 A5 JP 2017060502A5 JP 2016226816 A JP2016226816 A JP 2016226816A JP 2016226816 A JP2016226816 A JP 2016226816A JP 2017060502 A5 JP2017060502 A5 JP 2017060502A5
- Authority
- JP
- Japan
- Prior art keywords
- promoter
- gene
- nucleic acid
- acid construct
- allele
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108090000623 proteins and genes Proteins 0.000 claims 22
- 238000000034 method Methods 0.000 claims 15
- 108700028369 Alleles Proteins 0.000 claims 9
- 239000003550 marker Substances 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 9
- 102000039446 nucleic acids Human genes 0.000 claims 9
- 150000007523 nucleic acids Chemical class 0.000 claims 9
- 230000006798 recombination Effects 0.000 claims 9
- 238000005215 recombination Methods 0.000 claims 9
- 238000011144 upstream manufacturing Methods 0.000 claims 8
- 108010052160 Site-specific recombinase Proteins 0.000 claims 6
- 102000018120 Recombinases Human genes 0.000 claims 5
- 108010091086 Recombinases Proteins 0.000 claims 5
- 108700008625 Reporter Genes Proteins 0.000 claims 5
- 210000004027 cell Anatomy 0.000 claims 5
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 5
- 108700019146 Transgenes Proteins 0.000 claims 4
- 210000001082 somatic cell Anatomy 0.000 claims 4
- 108010043121 Green Fluorescent Proteins Proteins 0.000 claims 3
- 102000004144 Green Fluorescent Proteins Human genes 0.000 claims 3
- 239000005090 green fluorescent protein Substances 0.000 claims 3
- 230000035897 transcription Effects 0.000 claims 3
- 238000013518 transcription Methods 0.000 claims 3
- LRFVTYWOQMYALW-UHFFFAOYSA-N 9H-xanthine Chemical compound O=C1NC(=O)NC2=C1NC=N2 LRFVTYWOQMYALW-UHFFFAOYSA-N 0.000 claims 2
- 102000003839 Human Proteins Human genes 0.000 claims 2
- 108090000144 Human Proteins Proteins 0.000 claims 2
- 108091081024 Start codon Proteins 0.000 claims 2
- 108010082025 cyan fluorescent protein Proteins 0.000 claims 2
- 239000003814 drug Substances 0.000 claims 2
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 claims 2
- 229940124597 therapeutic agent Drugs 0.000 claims 2
- 108091005957 yellow fluorescent proteins Proteins 0.000 claims 2
- 102000007469 Actins Human genes 0.000 claims 1
- 108010085238 Actins Proteins 0.000 claims 1
- 102100036475 Alanine aminotransferase 1 Human genes 0.000 claims 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 claims 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 claims 1
- 108010045123 Blasticidin-S deaminase Proteins 0.000 claims 1
- 108020004705 Codon Proteins 0.000 claims 1
- 208000035240 Disease Resistance Diseases 0.000 claims 1
- 101001091269 Escherichia coli Hygromycin-B 4-O-kinase Proteins 0.000 claims 1
- 108010060309 Glucuronidase Proteins 0.000 claims 1
- 102000053187 Glucuronidase Human genes 0.000 claims 1
- 102000002265 Human Growth Hormone Human genes 0.000 claims 1
- 108010000521 Human Growth Hormone Proteins 0.000 claims 1
- 239000000854 Human Growth Hormone Substances 0.000 claims 1
- 102000008100 Human Serum Albumin Human genes 0.000 claims 1
- 108091006905 Human Serum Albumin Proteins 0.000 claims 1
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 claims 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 claims 1
- 108090001061 Insulin Proteins 0.000 claims 1
- 102000004877 Insulin Human genes 0.000 claims 1
- 108010025815 Kanamycin Kinase Proteins 0.000 claims 1
- 108090000942 Lactalbumin Proteins 0.000 claims 1
- 102000004407 Lactalbumin Human genes 0.000 claims 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 claims 1
- 108060001084 Luciferase Proteins 0.000 claims 1
- 239000005089 Luciferase Substances 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims 1
- 241000700584 Simplexvirus Species 0.000 claims 1
- 101001091268 Streptomyces hygroscopicus Hygromycin-B 7''-O-kinase Proteins 0.000 claims 1
- 108091036066 Three prime untranslated region Proteins 0.000 claims 1
- 102000006601 Thymidine Kinase Human genes 0.000 claims 1
- 108020004440 Thymidine kinase Proteins 0.000 claims 1
- 108090000901 Transferrin Proteins 0.000 claims 1
- 102000004338 Transferrin Human genes 0.000 claims 1
- 108010027570 Xanthine phosphoribosyltransferase Proteins 0.000 claims 1
- 108010005774 beta-Galactosidase Proteins 0.000 claims 1
- 102000005936 beta-Galactosidase Human genes 0.000 claims 1
- 239000003114 blood coagulation factor Substances 0.000 claims 1
- 108010048367 enhanced green fluorescent protein Proteins 0.000 claims 1
- 108010021843 fluorescent protein 583 Proteins 0.000 claims 1
- 230000006801 homologous recombination Effects 0.000 claims 1
- 238000002744 homologous recombination Methods 0.000 claims 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 1
- 229940125396 insulin Drugs 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 210000003205 muscle Anatomy 0.000 claims 1
- 101150079312 pgk1 gene Proteins 0.000 claims 1
- 235000018102 proteins Nutrition 0.000 claims 1
- 102000004169 proteins and genes Human genes 0.000 claims 1
- 108010045647 puromycin N-acetyltransferase Proteins 0.000 claims 1
- 230000035945 sensitivity Effects 0.000 claims 1
- 230000001225 therapeutic effect Effects 0.000 claims 1
- 239000012581 transferrin Substances 0.000 claims 1
- 229940075420 xanthine Drugs 0.000 claims 1
- 235000021241 α-lactalbumin Nutrition 0.000 claims 1
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261730771P | 2012-11-28 | 2012-11-28 | |
| US61/730,771 | 2012-11-28 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015544195A Division JP6154479B2 (ja) | 2012-11-28 | 2013-11-26 | 選択マーカーを含まないクローン化された非ヒト動物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017060502A JP2017060502A (ja) | 2017-03-30 |
| JP2017060502A5 true JP2017060502A5 (https=) | 2017-08-03 |
| JP6227747B2 JP6227747B2 (ja) | 2017-11-08 |
Family
ID=49725406
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015544195A Expired - Fee Related JP6154479B2 (ja) | 2012-11-28 | 2013-11-26 | 選択マーカーを含まないクローン化された非ヒト動物 |
| JP2016226816A Expired - Fee Related JP6227747B2 (ja) | 2012-11-28 | 2016-11-22 | 選択マーカーを含まないクローン化された非ヒト動物 |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015544195A Expired - Fee Related JP6154479B2 (ja) | 2012-11-28 | 2013-11-26 | 選択マーカーを含まないクローン化された非ヒト動物 |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20140221734A1 (https=) |
| EP (1) | EP2925122A1 (https=) |
| JP (2) | JP6154479B2 (https=) |
| KR (1) | KR20150089004A (https=) |
| CN (1) | CN104918484A (https=) |
| AU (1) | AU2013352235A1 (https=) |
| BR (1) | BR112015012408A2 (https=) |
| CA (1) | CA2891163A1 (https=) |
| HK (1) | HK1211177A1 (https=) |
| IL (1) | IL238771A0 (https=) |
| MX (1) | MX353279B (https=) |
| MY (1) | MY193790A (https=) |
| RU (1) | RU2015122802A (https=) |
| SG (1) | SG11201503752SA (https=) |
| WO (1) | WO2014085501A1 (https=) |
| ZA (1) | ZA201503305B (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3418379B1 (en) | 2013-09-18 | 2020-12-09 | Kymab Limited | Methods, cells & organisms |
| JP6929527B2 (ja) * | 2015-03-04 | 2021-09-01 | 株式会社ポル・メド・テック | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
| CN105200086A (zh) * | 2015-10-22 | 2015-12-30 | 中国农业科学院北京畜牧兽医研究所 | 一种无选择标记的多基因复合性状基因工程猪的制备方法 |
| JP6928604B2 (ja) * | 2015-11-04 | 2021-09-01 | フェイト セラピューティクス，インコーポレイテッド | 万能性細胞のゲノム改変 |
| JP7504030B2 (ja) | 2018-06-13 | 2024-06-21 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 進行性骨化性線維異形成症のげっ歯類モデル |
| CN109504708A (zh) * | 2018-12-03 | 2019-03-22 | 江苏集萃药康生物科技有限公司 | 一种筛选标记自我删除的基因打靶载体及方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1375654A3 (en) * | 1998-11-02 | 2008-01-16 | GTC Biotherapeutics, Inc. | Transgenic and cloned mammals |
| US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
| JPWO2006035741A1 (ja) * | 2004-09-29 | 2008-05-15 | 伸弥 山中 | Ｅｓ細胞特異的発現遺伝子及びその利用 |
| WO2009121999A1 (es) * | 2008-04-04 | 2009-10-08 | Consejo Superior De Investigaciones Científicas | Promotor especifico de celulas troncales |
| WO2011020005A1 (en) * | 2009-08-14 | 2011-02-17 | Regeneron Pharmaceuticals, Inc. | miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE |
| CN102628061A (zh) * | 2012-04-09 | 2012-08-08 | 西北农林科技大学 | 一种hbd3 乳腺特异性表达载体及构建的重组细胞 |
-
2013
- 2013-11-26 WO PCT/US2013/072088 patent/WO2014085501A1/en not_active Ceased
- 2013-11-26 CA CA2891163A patent/CA2891163A1/en not_active Abandoned
- 2013-11-26 US US14/091,207 patent/US20140221734A1/en not_active Abandoned
- 2013-11-26 EP EP13802213.2A patent/EP2925122A1/en not_active Withdrawn
- 2013-11-26 CN CN201380060720.0A patent/CN104918484A/zh active Pending
- 2013-11-26 MY MYPI2015001216A patent/MY193790A/en unknown
- 2013-11-26 KR KR1020157012263A patent/KR20150089004A/ko not_active Withdrawn
- 2013-11-26 AU AU2013352235A patent/AU2013352235A1/en not_active Abandoned
- 2013-11-26 SG SG11201503752SA patent/SG11201503752SA/en unknown
- 2013-11-26 MX MX2015006810A patent/MX353279B/es active IP Right Grant
- 2013-11-26 JP JP2015544195A patent/JP6154479B2/ja not_active Expired - Fee Related
- 2013-11-26 RU RU2015122802A patent/RU2015122802A/ru not_active Application Discontinuation
- 2013-11-26 BR BR112015012408A patent/BR112015012408A2/pt not_active IP Right Cessation
- 2013-11-26 HK HK15112184.4A patent/HK1211177A1/xx unknown
-
2015
- 2015-05-12 ZA ZA2015/03305A patent/ZA201503305B/en unknown
- 2015-05-12 IL IL238771A patent/IL238771A0/en unknown
-
2016
- 2016-11-22 JP JP2016226816A patent/JP6227747B2/ja not_active Expired - Fee Related
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