JP2016527896A5 - - Google Patents
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- Publication number
- JP2016527896A5 JP2016527896A5 JP2016533915A JP2016533915A JP2016527896A5 JP 2016527896 A5 JP2016527896 A5 JP 2016527896A5 JP 2016533915 A JP2016533915 A JP 2016533915A JP 2016533915 A JP2016533915 A JP 2016533915A JP 2016527896 A5 JP2016527896 A5 JP 2016527896A5
- Authority
- JP
- Japan
- Prior art keywords
- inosine
- dna
- exonuclease
- primer
- primer solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 25
- 229930010555 Inosine Natural products 0.000 claims 15
- 229960003786 inosine Drugs 0.000 claims 15
- 108020004414 DNA Proteins 0.000 claims 11
- 108060002716 Exonuclease Proteins 0.000 claims 9
- 102000013165 exonuclease Human genes 0.000 claims 9
- 230000002950 deficient Effects 0.000 claims 8
- 108010068698 spleen exonuclease Proteins 0.000 claims 8
- 230000004544 DNA amplification Effects 0.000 claims 7
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 7
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 7
- 238000000034 method Methods 0.000 claims 7
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims 6
- 239000011541 reaction mixture Substances 0.000 claims 6
- 239000002773 nucleotide Substances 0.000 claims 5
- 125000003729 nucleotide group Chemical group 0.000 claims 5
- 108091093088 Amplicon Proteins 0.000 claims 4
- 108010042407 Endonucleases Proteins 0.000 claims 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims 4
- 101710163270 Nuclease Proteins 0.000 claims 4
- 108020004707 nucleic acids Proteins 0.000 claims 4
- 102000039446 nucleic acids Human genes 0.000 claims 4
- 150000007523 nucleic acids Chemical class 0.000 claims 4
- 238000006073 displacement reaction Methods 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 2
- 101710116602 DNA-Binding protein G5P Proteins 0.000 claims 2
- 102100031780 Endonuclease Human genes 0.000 claims 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 229920001917 Ficoll Polymers 0.000 claims 2
- 101710162453 Replication factor A Proteins 0.000 claims 2
- 101710176758 Replication protein A 70 kDa DNA-binding subunit Proteins 0.000 claims 2
- 101710176276 SSB protein Proteins 0.000 claims 2
- 101710126859 Single-stranded DNA-binding protein Proteins 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 230000000415 inactivating effect Effects 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 102000053602 DNA Human genes 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000005202 decontamination Methods 0.000 claims 1
- 230000003588 decontaminative effect Effects 0.000 claims 1
- -1 e.g. Chemical group 0.000 claims 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
- 235000011178 triphosphate Nutrition 0.000 claims 1
- 239000001226 triphosphate Substances 0.000 claims 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13/965,696 US20130323795A1 (en) | 2007-01-10 | 2013-08-13 | Endonuclase-assisted isothermal amplification using contamination-free reagents |
| US13/965,696 | 2013-08-13 | ||
| PCT/EP2014/067321 WO2015022359A1 (en) | 2013-08-13 | 2014-08-13 | Endonuclease-assisted isothermal amplification using contamination-free reagents |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019096038A Division JP2019165739A (ja) | 2013-08-13 | 2019-05-22 | コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016527896A JP2016527896A (ja) | 2016-09-15 |
| JP2016527896A5 true JP2016527896A5 (cg-RX-API-DMAC7.html) | 2017-09-21 |
| JP6786390B2 JP6786390B2 (ja) | 2020-11-25 |
Family
ID=51302997
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016533915A Active JP6786390B2 (ja) | 2013-08-13 | 2014-08-13 | コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅 |
| JP2019096038A Pending JP2019165739A (ja) | 2013-08-13 | 2019-05-22 | コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019096038A Pending JP2019165739A (ja) | 2013-08-13 | 2019-05-22 | コンタミネーションフリー試薬を使用したエンドヌクレアーゼ支援等温増幅 |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP3033431B1 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6786390B2 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2015022359A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20130323795A1 (en) | 2007-01-10 | 2013-12-05 | General Electric Company | Endonuclase-assisted isothermal amplification using contamination-free reagents |
| US9938568B2 (en) | 2013-07-26 | 2018-04-10 | General Electric Company | Ligase-assisted nucleic acid circularization and amplification |
| WO2015022359A1 (en) * | 2013-08-13 | 2015-02-19 | General Electric Company | Endonuclease-assisted isothermal amplification using contamination-free reagents |
| WO2016156071A1 (en) | 2015-03-30 | 2016-10-06 | F. Hoffmann-La Roche Ag | Methods to amplify highly uniform and less error prone nucleic acid libraries |
| CN108350492A (zh) * | 2015-11-05 | 2018-07-31 | 通用电气公司 | 连接酶辅助的核酸环化和扩增 |
| JP7096893B2 (ja) * | 2018-02-05 | 2022-07-06 | エフ．ホフマン－ラ ロシュ アーゲー | 単一分子のための一本鎖環状ｄｎａ鋳型の作製 |
| JP2023516827A (ja) * | 2020-03-06 | 2023-04-20 | ライフ テクノロジーズ コーポレイション | 高配列忠実度の核酸合成およびアセンブリ |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2176193A1 (en) * | 1995-05-22 | 1996-11-23 | John Wesley Backus | Methods for polymerase chain reaction preamplification sterilization by exonucleases in the presence of phosphorothioated primers |
| US8361712B2 (en) * | 2007-12-17 | 2013-01-29 | General Electric Company | Contamination-free reagents for nucleic acid amplification |
| WO2006125267A1 (en) * | 2005-05-26 | 2006-11-30 | Human Genetic Signatures Pty Ltd | Isothermal strand displacement amplification using primers containing a non-regular base |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| JP5420412B2 (ja) * | 2006-09-14 | 2014-02-19 | アイビス バイオサイエンシズ インコーポレイティッド | 病原体の同定のための標的全ゲノム増幅方法 |
| US8202972B2 (en) * | 2007-01-10 | 2012-06-19 | General Electric Company | Isothermal DNA amplification |
| CN104583413B (zh) * | 2012-06-29 | 2018-10-19 | 通用电气公司 | 用于自rna模板开始的等温dna扩增试剂盒 |
| WO2015022359A1 (en) * | 2013-08-13 | 2015-02-19 | General Electric Company | Endonuclease-assisted isothermal amplification using contamination-free reagents |
-
2014
- 2014-08-13 WO PCT/EP2014/067321 patent/WO2015022359A1/en not_active Ceased
- 2014-08-13 EP EP14750502.8A patent/EP3033431B1/en active Active
- 2014-08-13 JP JP2016533915A patent/JP6786390B2/ja active Active
-
2019
- 2019-05-22 JP JP2019096038A patent/JP2019165739A/ja active Pending
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