JP2016519950A - 操作されたインテインを使用してペプチドを生産する方法 - Google Patents

操作されたインテインを使用してペプチドを生産する方法 Download PDF

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JP2016519950A
JP2016519950A JP2016516145A JP2016516145A JP2016519950A JP 2016519950 A JP2016519950 A JP 2016519950A JP 2016516145 A JP2016516145 A JP 2016516145A JP 2016516145 A JP2016516145 A JP 2016516145A JP 2016519950 A JP2016519950 A JP 2016519950A
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Prior art keywords
intein
seq
fusion protein
peptide
target peptide
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Japanese (ja)
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JP2016519950A5 (enExample
Inventor
アラン・クリスチャン・ショー
イェンス・クリスチャン・ノリルド
ルイーセ・アルベルトセン
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Novo Nordisk AS
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Novo Nordisk AS
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/006—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length of peptides containing derivatised side chain amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K7/00—Peptides having 5 to 20 amino acids in a fully defined sequence; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
JP2016516145A 2013-05-31 2014-05-28 操作されたインテインを使用してペプチドを生産する方法 Withdrawn JP2016519950A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP13170009 2013-05-31
EP13170009.8 2013-05-31
US201361833046P 2013-06-10 2013-06-10
US61/833,046 2013-06-10
PCT/EP2014/061048 WO2014191455A1 (en) 2013-05-31 2014-05-28 Methods for producing peptides using engineered inteins

Publications (2)

Publication Number Publication Date
JP2016519950A true JP2016519950A (ja) 2016-07-11
JP2016519950A5 JP2016519950A5 (enExample) 2017-07-13

Family

ID=48536725

Family Applications (1)

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JP2016516145A Withdrawn JP2016519950A (ja) 2013-05-31 2014-05-28 操作されたインテインを使用してペプチドを生産する方法

Country Status (5)

Country Link
US (1) US9670257B2 (enExample)
EP (1) EP3003357A1 (enExample)
JP (1) JP2016519950A (enExample)
CN (1) CN105263509A (enExample)
WO (1) WO2014191455A1 (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9771412B2 (en) * 2014-10-31 2017-09-26 Wisconsin Alumni Research Foundation Engineered intein for improved production of protein-intein fusions
SI3215614T1 (sl) 2014-11-03 2019-11-29 Merck Patent Gmbh Topni fuzijski proteini inteina in postopki za čiščenje biomolekul
US11136360B2 (en) 2018-12-19 2021-10-05 Tsinghua University Fusion proteins comprising a Mtu ΔI-CM intein variant and methods of protein purification
WO2021047559A1 (zh) * 2019-09-09 2021-03-18 武汉友芝友生物制药有限公司 一种断裂型内含肽、使用其的重组多肽的制备方法
GB202300816D0 (en) 2023-01-19 2023-03-08 Isomerase Therapeutics Ltd Novel method

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9708918D0 (en) 1997-05-01 1997-06-25 Ppl Therapeutics Scotland Ltd Methods
EP1090132A1 (en) 1998-06-26 2001-04-11 PPL Therapeutics (Scotland) Limited Methods for production of recombinant polypeptides
DE69931382T2 (de) 1998-09-30 2007-05-03 New England Biolabs, Inc., Ipswich Intein vermittelte peptidligation
FI20021726A0 (fi) 2002-09-27 2002-09-27 Ctt Cancer Targeting Tech Oy Menetelmä peptidien tuottamiseksi
WO2012036962A2 (en) 2010-09-13 2012-03-22 Amylin Pharmaceuticals, Inc. C-terminal amidation of polypeptides
EP2665744B1 (en) 2011-01-20 2015-04-08 University Of Rochester Macrocyclic compounds with a hybrid peptidic/non-peptidic backbone and methods for their preparation
EP2761006B1 (en) 2011-09-28 2016-12-14 Zera Intein Protein Solutions, S.L. Split inteins and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J. BACTERIOLOGY, VOL. 179, NO. 20, (1997), P. 6378-6382, JPN6018010408, ISSN: 0003763042 *
J. MOL. BIOL., VOL. 354, (2005), P. 916-926, JPN6018010407, ISSN: 0003763040 *
NATURE BIOTECHNOLOGY, VOL. 19, (2001), P. 974-977, JPN6018010410, ISSN: 0003763043 *
PROTEIN EXPRESSION AND PURIFICATION, VOL. 50, (2006), P. 74-81, JPN6018010405, ISSN: 0003763041 *

Also Published As

Publication number Publication date
WO2014191455A1 (en) 2014-12-04
US9670257B2 (en) 2017-06-06
CN105263509A (zh) 2016-01-20
EP3003357A1 (en) 2016-04-13
US20160096872A1 (en) 2016-04-07

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