JP2016166774A - Dispensation device, analyzer having the same, and dispensation method - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a dispensation device capable of satisfactorily determining, with an inexpensive configuration, whether or not a temperature of a reagent is appropriate, and also to provide an analyzer having the dispensation device, and a dispensation method.SOLUTION: A temperature sensor 52 for detecting a temperature of a probe 5 is used to detect the temperature of the probe 5 when absorbing or discharging a reagent by the probe 5, and according to the temperature, there is determined whether or not a temperature of the reagent is appropriate. In a configuration where the probe 5 is to be heated, usually, the temperature sensor 52 for detecting the temperature of the probe 5 is installed, therefore the temperature sensor 52 can be used to determine whether or not the temperature of the reagent is appropriate.SELECTED DRAWING: Figure 3

Description

  The present invention relates to a dispensing apparatus for aspirating a reagent with a heated probe, an analyzer equipped with the same, and a dispensing method.

  When measuring the blood coagulation reaction, a sample is mixed with a reagent, and the sample is maintained at a temperature close to human body temperature (for example, 37 ° C.). Usually, the reagent is cooled to maintain its stability and kept at a temperature lower than the human body temperature (for example, 6 ° C.).

  The reagent is aspirated by, for example, a probe and mixed by being discharged into the sample. The probe is heated, and the reagent sucked into the probe is heated to a temperature close to human body temperature (for example, 37 ° C.) and then discharged into the sample. Specifically, based on a detection signal from a temperature sensor that detects the temperature of the probe, feedback control is performed so that the temperature of the probe approaches the set temperature (see, for example, Patent Document 1 below). .

Japanese Patent Laid-Open No. 11-153603

  However, in the conventional configuration as described above, for example, when the cooling unit for cooling the reagent is out of order, the temperature of the reagent when discharged from the probe may be too high. That is, in the case of a configuration in which the reagent aspirated to the probe is heated for a predetermined time and then discharged, the temperature of the reagent when discharged from the probe depends on the set temperature of the probe and the temperature of the reagent before aspiration. It becomes. Therefore, when the temperature of the reagent at the time of aspiration is high due to a failure of the cooling unit, the temperature of the reagent discharged from the probe after a certain time from the aspiration may become too high.

  The blood coagulation reaction has a short measurement time, for example, about 20 seconds, and is highly temperature-dependent. Therefore, if the temperature of the reagent when it is discharged from the probe and mixed with the sample is high, the coagulation time is shortened and measured. The result will be greatly affected. In addition, even when the temperature of the reagent when it is discharged from the probe and mixed with the sample is low, the coagulation time is extended, so that the measurement result is similarly greatly affected.

  Therefore, it is conceivable to determine whether or not the temperature of the reagent is appropriate by directly detecting the temperature of the reagent in the cooling unit with a temperature sensor. However, when the temperature sensor is brought into contact with the reagent, there is a possibility that foreign substances are mixed in the reagent. Further, when a plurality of types of reagents are used, it is necessary to provide a temperature sensor for each reagent or to detect the temperature of each reagent by washing the temperature sensor.

  For the above reasons, it is difficult to directly detect the temperature of the reagent with a temperature sensor. In addition, it is conceivable to provide a sensor that detects the temperature of the reagent in a non-contact manner. However, when such an expensive sensor is separately provided, there is a problem that the manufacturing cost increases.

  The present invention has been made in view of the above circumstances, and a dispensing device that can satisfactorily determine whether or not the temperature of a reagent is appropriate with an inexpensive configuration, an analyzer equipped with the same, and dispensing It aims to provide a method.

  The dispensing apparatus according to the present invention includes a probe, a heating unit, a temperature sensor, a probe control unit, and a temperature determination unit. The probe aspirates a reagent. The heating unit heats the probe. The temperature sensor detects the temperature of the probe. The probe control unit controls aspiration and discharge of the reagent by the probe. The temperature determination unit determines whether the temperature of the reagent is appropriate based on the temperature of the probe detected by the temperature sensor when the reagent is aspirated or discharged by the probe.

  According to such a configuration, the temperature of the probe is detected at the time of aspiration or discharge of the reagent by the probe using the temperature sensor that detects the temperature of the probe, and whether or not the temperature of the reagent is appropriate is determined based on the temperature. Can be determined. That is, the temperature of the reagent can be indirectly detected based on the temperature of the probe, instead of directly detecting the temperature of the reagent.

  In the case of a configuration in which the probe is heated, a temperature sensor that detects the temperature of the probe is usually provided, so that it can be determined whether or not the temperature of the reagent is appropriate using the temperature sensor. . Therefore, it is possible to satisfactorily determine whether or not the temperature of the reagent is appropriate with an inexpensive configuration.

  The dispensing apparatus may further include a cooling unit that cools the reagent. In this case, the temperature determination unit determines that the temperature of the reagent is not appropriate when the amount of decrease in the temperature of the probe detected by the temperature sensor when the reagent is aspirated by the probe is less than a threshold value. Also good.

  According to such a configuration, it is possible to satisfactorily determine whether or not the temperature of the reagent is appropriate based on the amount of decrease in the temperature of the probe detected by the temperature sensor when the reagent is aspirated by the probe. That is, when the reagent is cooled to an appropriate temperature, the amount of decrease in the temperature of the probe when the reagent is aspirated with the probe is relatively large. When the reagent temperature is high, the amount of decrease in the probe temperature when the reagent is aspirated by the probe is relatively small. By using such a decrease amount of the probe temperature as a reference, it is possible to satisfactorily determine whether or not the temperature of the reagent is appropriate.

  The dispensing apparatus may further include a notification unit that notifies the fact when the temperature determination unit determines that the temperature of the reagent is not appropriate.

  According to such a configuration, when it is determined that the temperature of the reagent is not appropriate, the operator is notified of this, so that the operator can confirm that the measurement result of the sample mixed with the reagent is not appropriate. Can be recognized. It is also possible to prevent an operator who has recognized that the temperature of the reagent is not appropriate from performing an inappropriate measurement in advance by stopping the operation of the dispensing device.

  The dispensing apparatus may further include a restriction processing unit that restricts the discharge of the reagent by the probe control unit when the temperature determination unit determines that the temperature of the reagent is not appropriate.

  According to such a configuration, when it is determined that the temperature of the reagent is not appropriate, the discharge of the reagent is automatically restricted, so that the reagent is not mixed with the sample. Therefore, it is possible to reliably prevent an inappropriate measurement from being performed.

  The analyzer according to the present invention includes the dispensing device and a mixing unit that mixes the reagent discharged from the probe with a sample.

  The dispensing method according to the present invention includes a suction step, a discharge step, a temperature detection step, and a temperature determination step. In the aspirating step, the reagent is aspirated by the heated probe. In the discharge step, the reagent sucked in the suction step is discharged from the probe. In the temperature detection step, the temperature of the probe is detected when the reagent is aspirated or discharged by the probe. In the temperature determining step, it is determined whether the temperature of the reagent is appropriate based on the temperature of the probe detected in the temperature detecting step.

  The reagent may be cooled. In this case, in the temperature determination step, it is determined that the temperature of the reagent is not appropriate when the amount of decrease in the probe temperature detected in the temperature detection step when the reagent is aspirated by the probe is less than a threshold value. May be.

  The dispensing method may further include a notification step of notifying that when the temperature determination step determines that the temperature of the reagent is not appropriate.

  The dispensing method may further include a restriction processing step for restricting the discharge of the reagent in the discharge step when it is determined in the temperature determination step that the temperature of the reagent is not appropriate.

It is a schematic plan view which shows the structural example of the analyzer provided with the dispensing apparatus which concerns on one Embodiment of this invention. It is a schematic sectional drawing for demonstrating the operation | movement at the time of aspirating a reagent from a container, and has shown the state before making the front-end | tip part of a probe approach in a container. It is a schematic sectional drawing for demonstrating the operation | movement at the time of aspirating a reagent from a container, and has shown the state which made the front-end | tip part of a probe approach in a container. It is a block diagram which shows an example of the electrical constitution of a dispensing apparatus. It is a figure which shows an example of the temperature change of a probe when the temperature of a reagent is appropriate. It is a figure which shows an example of the temperature change of a probe when the temperature of a reagent is not appropriate. It is a flowchart which shows an example of the process by the control part at the time of dispensing the reagent in a container.

  FIG. 1 is a schematic plan view showing a configuration example of an analyzer equipped with a dispensing apparatus 100 according to an embodiment of the present invention. This dispensing apparatus 100 is used, for example, when measuring the coagulation reaction of blood. By discharging the reagent sucked from the container 1 to the mixing unit 2, the reagent is applied to the sample in the mixing unit 2. Can be mixed. The mixing unit 2 can measure the blood coagulation reaction by maintaining the sample mixed with the reagent at a temperature close to the human body temperature (for example, 37 ° C.).

  The container 1 containing the reagent is held by the container holding unit 3. In the present embodiment, the container holding part 3 is rotatable about the rotation shaft 31, and a plurality of containers 1 are held side by side in an arc shape around the rotation shaft 31. However, the container holding unit 3 may be configured not to rotate or may be configured to hold only one container 1.

  The container holding unit 3 is cooled by the cooling unit 4. The cooling unit 4 cools the container 1 through the container holding unit 3 to keep the reagent in the container 1 at a temperature lower than the human body temperature (for example, 6 ° C.). Thereby, the stability of the reagent in the container 1 can be maintained, and the sample can be favorably analyzed using the reagent. However, the cooling unit 4 is not limited to a configuration that cools the container 1 via the container holding unit 3, and may be a configuration that directly cools the container 1, for example.

  The reagent in the container 1 is aspirated by the probe 5. The probe 5 is held by an arm 6, and by displacing the arm 6, the tip of the probe 5 can enter the container 1 and the reagent in the container 1 can be aspirated. In the present embodiment, the arm 6 is held so as to be rotatable about the rotation shaft 61 and can be displaced in the vertical direction along the rotation shaft 61.

  The probe 5 held by the arm 6 that rotates about the rotating shaft 61 is displaced in the horizontal direction along an arcuate track L centered on the rotating shaft 61. The container 1 held by the container holding unit 3 can be sequentially transported on the track L of the probe 5 by rotating the container holding unit 3 around the rotation shaft 31. The mixing unit 2 is also located on the track L of the probe 5.

  2A and 2B are schematic cross-sectional views for explaining an operation when a reagent is aspirated from the container 1. When the reagent is aspirated from the container 1, the probe 5 is moved above the container 1 by rotating the arm 6 as shown in FIG. 2A. Thereafter, by displacing the probe 5 downward, as shown in FIG. 2B, the tip of the probe 5 enters the container 1, and the reagent is aspirated with the tip inserted into the reagent in the container 1. .

  Thereafter, the probe 5 is displaced upward and returned to the state shown in FIG. 2A, and then the arm 6 is rotated to move the probe 5 horizontally along the trajectory L to above the mixing unit 2. Then, by displacing the probe 5 downward, the tip of the probe 5 enters the mixing unit 2 and the reagent is discharged into the mixing unit 2 to mix the sample and the reagent in the mixing unit 2. be able to.

  FIG. 3 is a block diagram showing an example of the electrical configuration of the dispensing apparatus 100. As shown in FIG. In addition to the above-described units, the dispensing device 100 includes, for example, a probe driving unit 7, a control unit 8, a display unit 9, and the like. The control unit 8 includes, for example, a CPU (Central Processing Unit), and when the CPU executes a program, the probe control unit 81, the temperature control unit 82, the temperature determination unit 83, the limit processing unit 84, and the display control. It functions as the unit 85 or the like.

  The probe driving unit 7 includes a motor 71, a pump 72, and the like. The probe control unit 81 can drive the motor 71 to rotate the arm 6 around the rotation shaft 61 or move the arm 6 up and down along the rotation shaft 61. In addition, the probe control unit 81 can cause the reagent to be sucked into the probe 5 and the reagent in the probe 5 to be discharged by controlling the driving of the pump 72.

  The probe 5 includes a heating wire 51 and a temperature sensor 52. The heating wire 51 is composed of, for example, a nichrome wire provided inside the probe 5, and the probe 5 can be heated by generating heat by passing a current through the heating wire 51. That is, the heating wire 51 constitutes a heating unit for heating the probe 5. However, the probe 5 may be configured to be heated using a heating unit other than the heating wire 51.

  The temperature sensor 52 detects the temperature of the probe 5. The temperature control unit 82 feedback-controls the amount of current supplied to the heating wire 51 so that the temperature of the probe 5 detected by the temperature sensor 52 approaches the set temperature. The set temperature may be a predetermined constant temperature, or may be configured to be set to an arbitrary temperature by an operator's setting operation.

  The temperature determination unit 83 determines whether the reagent temperature is appropriate based on the temperature of the probe 5 detected by the temperature sensor 52. In the present embodiment, when the reagent is aspirated by the probe 5, the temperature of the probe 5 is detected by the temperature sensor 52, and it is determined whether or not the temperature of the reagent is appropriate based on the detection signal.

  As described above, in the present embodiment, the temperature sensor 52 that detects the temperature of the probe 5 is used to detect the temperature of the probe 5 when the reagent is aspirated by the probe 5, and whether or not the temperature of the reagent is appropriate based on the temperature. Can be determined. That is, the temperature of the reagent can be indirectly detected based on the temperature of the probe 5 instead of directly detecting the temperature of the reagent.

  In the case of the configuration in which the probe 5 is heated as in the present embodiment, since the temperature sensor 52 that normally detects the temperature of the probe 5 is provided, the temperature of the reagent is measured using the temperature sensor 52. Whether or not is appropriate. Therefore, it is possible to satisfactorily determine whether or not the temperature of the reagent is appropriate with an inexpensive configuration. Further, since it is possible to determine whether or not the temperature of the reagent is appropriate when the reagent is being dispensed, it is not necessary to perform the determination separately other than at the time of dispensing the reagent.

  The restriction processing unit 84 restricts the operation of the probe 5 controlled by the probe control unit 81 based on the determination result by the temperature determination unit 83. Specifically, when the temperature determination unit 83 determines that the reagent temperature is not appropriate, the restriction processing unit 84 stops the driving of the motor 71 or the pump 72 by the probe control unit 81, thereby discharging the reagent. Limit. Thereby, since the discharge of the reagent whose temperature is not appropriate is automatically restricted, the reagent is not mixed with the sample, and it is possible to reliably prevent an inappropriate measurement from being performed.

  The display control unit 85 controls the operation of the display unit 9. The display part 9 is comprised, for example with the liquid crystal display, and the various information regarding operation | movement of the dispensing apparatus 100 is displayed. When the temperature determination unit 83 determines that the reagent temperature is not appropriate, the display control unit 85 notifies the operator by displaying the fact on the display unit 9.

  As described above, when it is determined that the temperature of the reagent is not appropriate, the operator is notified of this, so that the operator recognizes that the measurement result of the sample mixed with the reagent is not appropriate. Can do. In addition, it is possible to prevent an operator who recognizes that the temperature of the reagent is not appropriate from performing an inappropriate measurement by stopping the operation of the dispensing apparatus 100 in advance.

  However, the notifying unit for notifying that the temperature of the reagent is not appropriate is not limited to the one configured by the display control unit 85 and the display unit 9. That is, the notification unit may be configured to notify the worker in another mode such as voice when it is determined that the temperature of the reagent is not appropriate.

  FIG. 4A is a diagram illustrating an example of a temperature change of the probe 5 when the temperature of the reagent is appropriate. In this example, the temperature of the probe 5 detected by the temperature sensor 52 until the reagent kept at 6 ° C. as an appropriate temperature is sucked from the container 1 to the probe 5 and discharged from the mixing unit 2 is temporal. Changes are shown.

  The probe 5 is heated by the heating wire 51 so as to be substantially constant between 51.0 and 51.5 ° C., for example. Therefore, when the suction of the reagent at 6 ° C. which is lower than the temperature of the probe 5 is started (timing A), the temperature of the probe 5 gradually decreases. When the aspiration by the probe 5 is completed (timing B), the temperature of the probe 5 becomes the lowest, and thereafter, the temperature of the probe 5 gradually increases until the aspirated reagent is discharged (timing C). It will be.

  In this example, the descending amount from the temperature when the suction by the probe 5 is started (about 51.2 ° C.) to the temperature when the suction by the probe 5 is finished (about 49.2 ° C.) is about 2 ° C. It has become. When the aspirated reagent is discharged from the probe 5 (timing C), the temperature of the probe 5 is about 50.4 ° C.

  FIG. 4B is a diagram illustrating an example of a temperature change of the probe 5 when the temperature of the reagent is not appropriate. In this example, when the reagent is 17 ° C. higher than the appropriate temperature, the probe is detected by the temperature sensor 52 until the reagent is sucked from the container 1 to the probe 5 and discharged from the mixing unit 2. A temperature change of 5 is shown.

  As in the case of FIG. 4A, the probe 5 is heated by the heating wire 51 so as to be substantially constant, for example, between 51.0 and 51.5 ° C. When the suction of the reagent at 17 ° C. which is lower than the temperature of the probe 5 is started (timing A), the temperature of the probe 5 gradually decreases, but since the reagent is higher than the appropriate temperature, the speed is slower than in the case of FIG. As a result, the temperature of the probe 5 decreases. When the aspiration by the probe 5 is completed (timing B), the temperature of the probe 5 becomes the lowest, and thereafter, the temperature of the probe 5 gradually increases until the aspirated reagent is discharged (timing C). It will be.

  In this example, the descending amount from the temperature when the suction by the probe 5 is started (about 51.3 ° C.) to the temperature when the suction by the probe 5 is finished (about 51.0 ° C.) is about 0.3. It is ℃. When the sucked sample is discharged from the probe 5 (timing C), the temperature of the probe 5 is increased to about 51.6 ° C.

  Thus, when the reagent is higher than the appropriate temperature, the temperature decrease amount of the probe 5 detected by the temperature sensor 52 when the reagent is aspirated by the probe 5 is smaller than when the reagent is at the appropriate temperature. Become. That is, when the reagent is cooled to an appropriate temperature, the amount of decrease in the temperature of the probe 5 when the reagent is aspirated by the probe 5 is relatively large. For example, the cooling unit 4 breaks down, When the temperature of the reagent at the time of aspiration is high, the amount of decrease in the temperature of the probe 5 when the reagent is aspirated by the probe 5 is relatively small.

  Therefore, it is possible to determine whether or not the temperature of the reagent is appropriate by comparing the amount of decrease with a threshold value. For example, when the descending amount is less than the threshold value, the temperature determination unit 83 determines that the temperature of the reagent is not appropriate. The threshold is preferably 0.5 to 1.5 ° C, more preferably about 1.0 ° C. Thereby, it is possible to satisfactorily determine whether or not the temperature of the reagent is appropriate based on the amount of decrease in the temperature of the probe 5 detected by the temperature sensor 52 when the probe 5 sucks the reagent.

  FIG. 5 is a flowchart showing an example of processing by the control unit 8 when dispensing the reagent in the container 1. When dispensing the reagent in the container 1, first, the tip of the heated probe 5 is inserted into the reagent in the container 1, and the probe 5 starts aspirating the reagent (step S101). . At this time, the temperature sensor 52 detects the temperature of the probe 5 (step S102).

  Thereafter, when a certain time has passed (Yes in step S103), the aspiration of the reagent by the probe 5 is completed (step S104), whereby a certain amount of the reagent is aspirated into the probe 5. The fixed time is a value that changes according to the amount of the reagent to be aspirated. For example, when the operator sets the amount of the reagent, the fixed time changes according to the set value. When the reagent suction is completed, the temperature of the probe 5 is detected again by the temperature sensor 52 (step S105).

  The process from the start of reagent aspiration (step S101) to the end of aspiration (step S104) constitutes an aspiration step in which the reagent is aspirated by the heated probe 5. Further, the process of detecting the temperature of the reagent in steps S102 and S105 constitutes a temperature detection step of detecting the temperature of the probe 5 when the probe 5 is aspirating the reagent.

  Based on the temperature of the probe 5 thus detected, the amount of decrease from the temperature of the reagent detected in step S102 to the temperature of the reagent detected in step S105 is compared with a predetermined threshold value. (Step S106). This step S106 constitutes a temperature determination step for determining whether or not the temperature of the reagent is appropriate.

  If the descending amount is equal to or greater than the threshold (Yes in step S106), it is determined that the reagent temperature is appropriate, and the arm 6 is moved from the container 1 to the mixing unit 2 (step S107). Then, the reagent and the reagent are mixed in the mixing unit 2 by discharging the reagent from the probe 5 into the mixing unit 2 (step S108: discharge step).

  On the other hand, when the amount of decrease is less than the threshold (No in step S106), it is determined that the temperature of the reagent is not appropriate (step S109). In this case, the operator is notified of the abnormality by displaying on the display unit 9 that the reagent temperature is not appropriate (step S110: notification step). Further, by stopping the driving of the motor 71 and the pump 72 in the probe driving unit 7 thereafter, the discharge of the reagent to the mixing unit 2 is restricted (step S111: restriction process step).

  The limiting process step in step S111 is not limited to the configuration in which both the motor 71 and the pump 72 are stopped. For example, the operation of either the motor 71 or the pump 72 is performed as long as the reagent is not mixed with the sample. It may be configured to stop or change. Even if it is determined that the temperature of the reagent is not appropriate and the reagent is not mixed with the sample, the sample cannot be reused and is discarded.

  However, the process (step S111) for limiting the discharge of the reagent when it is determined that the temperature of the reagent is not appropriate may be omitted. Further, the process of notifying the abnormality when it is determined that the temperature of the reagent is not appropriate (step S110) can be omitted.

  In the above embodiment, the configuration has been described in which it is determined whether or not the temperature of the reagent is appropriate based on the amount of decrease in the temperature of the probe 5 detected by the temperature sensor 52 when the probe 5 sucks the reagent. . However, the present invention is not limited to such a configuration. For example, when the temperature of the probe 5 when the reagent is aspirated by the probe 5 (timing B in FIGS. 4A and 4B) is equal to or higher than a predetermined threshold value, It may be configured to determine that is not appropriate.

  Further, the configuration is not limited to determining whether or not the temperature of the reagent is appropriate when the reagent is aspirated by the probe 5. For example, based on the temperature of the probe 5 detected by the temperature sensor 52 when the reagent is discharged, It may be configured to determine whether the temperature is appropriate. In this case, for example, the temperature of the probe 5 from when the reagent is aspirated by the probe 5 (timing B in FIGS. 4A and 4B) to when the aspirated reagent is discharged (timing C in FIGS. 4A and 4B). The configuration may be such that it is determined whether or not the temperature of the reagent is appropriate based on the amount of the increase. Alternatively, for example, when the temperature of the probe 5 at the time of discharging the reagent from the probe 5 is equal to or higher than a predetermined threshold, it may be determined that the temperature of the reagent is not appropriate.

  In the above embodiment, the configuration for detecting the case where the reagent is higher than the appropriate temperature has been described. However, the present invention is not limited to this. For example, the configuration is such that the case where the reagent is lower than the appropriate temperature is detected. Also good. In this case, for example, when the amount of decrease in the temperature of the probe 5 detected by the temperature sensor 52 when the reagent is aspirated by the probe 5 is equal to or greater than a predetermined threshold, the temperature of the reagent is not appropriate (lower than the appropriate temperature). ) May be determined. Further, for example, when the probe 5 is aspirated by the probe 5 (timing B in FIGS. 4A and 4B) and the temperature of the probe 5 is lower than a predetermined threshold, it is determined that the reagent temperature is not appropriate. It may be a configuration.

  The reagent is not limited to the configuration that is cooled by the cooling unit 4, but may be configured such that the reagent is set in the dispensing device in a cooled state in advance. In other words, it is not a configuration that uses the reagent while keeping the reagent in the dispensing device, but the worker sets the cooled reagent in the dispensing device or automatically transports it to the dispensing device. The configuration may be such that the cooled reagent is used in the dispensing apparatus.

  Moreover, although the above embodiment demonstrated the structure which determines whether the temperature of the reagent attracted | sucked to the probe 5 is appropriate when mixing a reagent with a sample, it is not restricted to this. For example, the temperature of the reagent is set in advance before mixing the reagent with the sample, such as when registering information on the reagent by setting the reagent in the dispensing device or checking the remaining amount of the reagent. If it is the structure which determines whether it is appropriate, it can prevent that a sample is wasted.

DESCRIPTION OF SYMBOLS 1 Container 2 Mixing part 3 Container holding part 4 Cooling part 5 Probe 6 Arm 7 Probe drive part 8 Control part 9 Display part 31 Rotating shaft 51 Heating wire 52 Temperature sensor 61 Rotating shaft 71 Motor 72 Pump 81 Probe control part 82 Temperature control part 83 Temperature Determination Unit 84 Restriction Processing Unit 85 Display Control Unit 100 Dispensing Device

Claims (9)

A probe for aspirating the reagent;
A heating unit for heating the probe;
A temperature sensor for detecting the temperature of the probe;
A probe control unit for controlling the suction and discharge of the reagent by the probe;
Dispensing characterized by comprising a temperature determination unit for determining whether or not the temperature of the reagent is appropriate based on the temperature of the probe detected by the temperature sensor at the time of aspiration or discharge of the reagent by the probe apparatus. A cooling unit for cooling the reagent;
The temperature determination unit determines that the temperature of the reagent is not appropriate when a decrease in the temperature of the probe detected by the temperature sensor when the reagent is aspirated by the probe is less than a threshold value. The dispensing device according to claim 1.   The dispensing apparatus according to claim 1, further comprising a notification unit that notifies the fact when the temperature determination unit determines that the temperature of the reagent is not appropriate.   4. The apparatus according to claim 1, further comprising: a restriction processing unit that restricts discharge of the reagent by the probe control unit when the temperature determination unit determines that the temperature of the reagent is not appropriate. The dispensing device described. A dispensing device according to any one of claims 1 to 4,
An analysis apparatus comprising: a mixing unit that mixes a reagent discharged from the probe with a sample. An aspiration step of aspirating the reagent with a heated probe;
A discharge step of discharging the reagent sucked in the suction step from the probe;
A temperature detection step of detecting the temperature of the probe at the time of aspiration or discharge of the reagent by the probe;
And a temperature determination step of determining whether or not the temperature of the reagent is appropriate based on the temperature of the probe detected in the temperature detection step. The reagent is cooled;
In the temperature determination step, when the amount of decrease in the temperature of the probe detected in the temperature detection step is less than a threshold when the reagent is aspirated by the probe, it is determined that the temperature of the reagent is not appropriate. The dispensing method according to claim 6.   The dispensing method according to claim 6 or 7, further comprising a notifying step of notifying that when the temperature of the reagent is determined to be inappropriate in the temperature determining step.   9. The method according to claim 6, further comprising a restriction processing step for restricting the discharge of the reagent in the discharge step when it is determined in the temperature determination step that the reagent temperature is not appropriate. Dispensing method.

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