JP2016154526A - Composition for lactic acid bacteria proliferation - Google Patents
Composition for lactic acid bacteria proliferation Download PDFInfo
- Publication number
- JP2016154526A JP2016154526A JP2015133930A JP2015133930A JP2016154526A JP 2016154526 A JP2016154526 A JP 2016154526A JP 2015133930 A JP2015133930 A JP 2015133930A JP 2015133930 A JP2015133930 A JP 2015133930A JP 2016154526 A JP2016154526 A JP 2016154526A
- Authority
- JP
- Japan
- Prior art keywords
- lactic acid
- acid bacteria
- barley
- composition
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
Description
本発明は、大麦の茎及び／又は葉の乾燥粉末を用いた乳酸菌増殖用組成物に関する。 The present invention relates to a composition for growing lactic acid bacteria using a dry powder of barley stems and / or leaves.
大麦は中央アジア原産とされ、イネ科に属する一年生又は越年生草本である。大麦は、穂形により、二条大麦と六条大麦などに大別される。二条大麦と六条大麦とでは、穂についている実の列数が異なり、穂を上から見ると二条大麦は２列に、六条大麦は６列に実がついている。六条大麦は、２〜３世紀に朝鮮を経て日本に渡来したとされ、雑穀として利用されるほか、麦茶の原料にも利用されている。一方、二条大麦は日本には欧米から明治時代に導入されたとされ、主に醸造用に用いられている。 Barley is native to Central Asia and is an annual or perennial herb belonging to the grass family. Barley is roughly classified into Nijo barley and Rojo barley depending on the shape of the spike. Nijo Barley and Rokujo Barley have different numbers of rows of fruit on the ears. Looking at the ear from the top, Nijo Barley has fruits in two rows and Rojo Barley has fruits in six rows. Rokujo barley is said to have come to Japan after passing through Korea in the 2nd to 3rd centuries. Nijo barley, on the other hand, was introduced in Japan during the Meiji period from Europe and America, and is mainly used for brewing.
乳酸菌は、グルコースなどの糖類から多量の乳酸を生成する細菌の総称であり、醤油、清酒、味噌などに見出される他、乳製品、穀類、腸などにも分布している。発酵乳、乳酸菌飲料、チーズなどの製造のために、乳酸菌の培養には獣乳を含む培地を使用することが多いが、乳酸菌の栄養要求性が厳格であるため、増殖に適さない菌株が多いという問題がある。このため、乳酸菌を増殖させるための物質として、培地中に、酵母エキス、麦芽エキス、ペプトン、アミノ酸などを添加することが知られているが、高価な上に培養条件が制限され、安価かつ簡便に、乳酸菌を増殖させることが難しいという問題がある。 Lactic acid bacteria are a general term for bacteria that produce a large amount of lactic acid from sugars such as glucose, and are found in soy sauce, sake, miso, etc., and are also distributed in dairy products, cereals, intestines and the like. In order to produce fermented milk, lactic acid bacteria beverages, cheese, etc., culture medium containing animal milk is often used for cultivation of lactic acid bacteria, but because of the strict nutritional requirements of lactic acid bacteria, there are many strains that are not suitable for growth. There is a problem. For this reason, it is known that yeast extract, malt extract, peptone, amino acid, etc. are added to the medium as a substance for growing lactic acid bacteria. In addition, there is a problem that it is difficult to grow lactic acid bacteria.
これらの課題を解決するため、本出願人は、安価かつ簡便に乳酸菌を増殖できる組成物として、特定の六条大麦の茎葉を用いた乳酸菌増殖用組成物を提案した（特許文献１）。 In order to solve these problems, the present applicant has proposed a composition for growing lactic acid bacteria using specific six-rowed barley stalks and leaves as a composition capable of growing lactic acid bacteria easily and inexpensively (Patent Document 1).
特許文献１によると、前記課題を解決しうる大麦の品種は非常に限られていた。しかしながら、大麦の普及品種は収量、病虫害抵抗性等の基準に基づき変遷するため、種子の入手困難性やそれに基づく大麦の茎葉の安定供給の困難性等の問題が生じることから、前記課題を解決しうるような更なる大麦品種の探索が求められていた。一方、大麦には３万種以上の膨大な数の品種が知られており（非特許文献１）、前記課題を解決しうる新たな大麦品種の探索は容易ではなかった。 According to Patent Document 1, barley varieties that can solve the above-mentioned problems are very limited. However, since popular barley varieties change based on standards such as yield and pest resistance, problems such as difficulty in obtaining seeds and difficulty in stable supply of barley foliage arise. There was a need to find more barley varieties that could be done. On the other hand, a huge number of varieties of 30,000 or more are known for barley (Non-patent Document 1), and it has not been easy to search for new barley varieties that can solve the above problems.
本発明の課題は、乳酸菌の増殖促進効果が高い乳酸菌増殖用組成物を提供することにある。 An object of the present invention is to provide a composition for growing lactic acid bacteria having a high effect of promoting the growth of lactic acid bacteria.
本発明者らは、上記課題について鋭意研究したところ、驚くべきことに、３万種以上ある大麦の品種の中でも、マンネンボシ、ダイシモチ、カシマムギ及びはるしずくから選ばれる少なくとも１の品種の大麦の茎葉の乾燥粉末を用いた乳酸菌増殖用組成物は、前記従来の乳酸菌増殖用組成物と比較して、乳酸菌増殖効果が高いことを見出し、本発明を完成させた。 As a result of intensive research on the above-mentioned problems, the present inventors have surprisingly found that among at least 30,000 kinds of barley varieties, at least one varieties of barley foliage selected from Mannenbosi, Daishimochi, Kashimumugi and Harushizuku The composition for growing lactic acid bacteria using this dry powder was found to have a higher effect of growing lactic acid bacteria than the conventional composition for growing lactic acid bacteria, and the present invention was completed.
本発明の要旨は以下のとおりである。
［１］大麦の茎及び／又は葉の乾燥粉末を含有することを特徴とする乳酸菌増殖用組成物であって、
前記大麦が、マンネンボシ、ダイシモチ、カシマムギ及びはるしずくから選ばれる少なくとも１の品種の大麦であり、前記乾燥粉末の安息角が２０°〜８０°である、乳酸菌増殖用組成物。
［２］前記乾燥粉末が粉砕末であって、前記粉砕末が３０〜２５０メッシュを通過するものである、［１］に記載の乳酸菌増殖用組成物。
［３］前記乾燥粉末が粉砕末であって、
前記粉砕末のメディアン径が１６μｍ以上であり、かつ、アスペクト比が２．０以下である、［１］又は［２］のいずれかに記載の乳酸菌増殖用組成物。
［４］前記乾燥粉末が粉砕末であって、
前記粉砕末のメディアン径が２０μｍ未満であり、かつ、ロジン・ラムラー式による分布定数ｎが１．６以上である、［１］又は［２］のいずれかに記載の乳酸菌増殖用組成物。
The gist of the present invention is as follows.
[1] A composition for growing lactic acid bacteria, comprising a dry powder of barley stems and / or leaves,
A composition for growing lactic acid bacteria, wherein the barley is at least one variety of barley selected from mannenbosi, daishimochi, oak and wheat, and the angle of repose of the dry powder is 20 ° to 80 °.
[2] The composition for growing lactic acid bacteria according to [1], wherein the dry powder is pulverized powder, and the pulverized powder passes through 30 to 250 mesh.
[3] The dry powder is pulverized powder,
The composition for growing lactic acid bacteria according to either [1] or [2], wherein the median diameter of the pulverized powder is 16 μm or more and the aspect ratio is 2.0 or less.
[4] The dry powder is pulverized powder,
The composition for growing lactic acid bacteria according to any one of [1] and [2], wherein the median diameter of the pulverized powder is less than 20 μm, and the distribution constant n according to the Rosin-Rammler equation is 1.6 or more.
本発明によれば、マンネンボシ、ダイシモチ、カシマムギ及びはるしずくから選ばれる少なくとも１の品種の大麦の茎葉の乾燥粉末を用いた乳酸菌増殖用組成物は、乳酸菌を良好に増殖させることができる。 According to the present invention, the composition for growing lactic acid bacteria using a dry powder of barley stalks and leaves of at least one variety selected from mannenbosi, daishimochi, oak and wheat can cultivate lactic acid bacteria satisfactorily.
以下、本発明を詳細に説明する。 Hereinafter, the present invention will be described in detail.
［１．大麦の茎及び／又は葉の乾燥粉末］
大麦の品種としては実に３万種以上の品種があることが知られているところ、本発明においては、マンネンボシ、ダイシモチ、カシマムギ及びはるしずく（以下、「特定品種」ともいう）から選ばれる少なくとも１の大麦の茎及び／又は葉の乾燥粉末を用いる。
[1. Barley stem and / or leaf dry powder]
It is known that there are actually more than 30,000 varieties as barley varieties. In the present invention, at least selected from Mannenbosi, Daishimochi, Kashimumugi, and Harusu Shizuku (hereinafter also referred to as “specific varieties”). 1 dry barley stem and / or leaf powder.
特定品種の大麦は、例えば精麦用として、具体的には、麦味噌、麦茶、焼酎、ビールなどの原料として一般的に用いられているものである。本発明においては、これらの品種のうち１種を単独で、又は２種以上を組み合わせて用いることができる。大麦の茎葉の乾燥粉末は、大麦の葉、茎又はその両方であり、葉及び茎はそれぞれその一部又は全部であってもよい。 Specific varieties of barley are, for example, generally used as raw materials for wheat, such as barley miso, barley tea, shochu, and beer. In the present invention, one of these varieties can be used alone, or two or more can be used in combination. The dry powder of barley stalks and leaves may be barley leaves, stems, or both, and the leaves and stems may be part or all of each.
特定品種の大麦の茎葉は、成熟期前、すなわち分けつ開始期から出穂開始前期に収穫されることが好ましい。大麦の茎葉は、収穫後、直ちに処理されることが好ましい。処理までに時間を要する場合、大麦の茎葉の変質を防ぐために低温貯蔵などの当業者が通常用いる貯蔵手段により貯蔵される。 The barley stalks and leaves of a specific variety are preferably harvested before the maturity period, that is, from the beginning of the splitting to the early stage of heading. Barley foliage is preferably processed immediately after harvesting. When processing takes time, it is stored by storage means commonly used by those skilled in the art, such as low-temperature storage, to prevent barley foliage alteration.
本発明で用いる特定品種の大麦の茎葉として、該茎葉から得られる各種の加工物、すなわち、特定品種の大麦の加工物を用いることができる。そのような加工物としては、例えば、茎葉の乾燥粉末、茎葉の粉砕物及びその乾燥粉末（以下、粉砕物の乾燥粉末のことを「粉砕末」ともいう）、茎葉の細片化物及びその乾燥粉末（以下、細片化物の乾燥粉末のことを「細片化末」ともいう）、茎葉の搾汁及びその乾燥粉末（以下、搾汁の乾燥粉末のことを「搾汁末」ともいう）、茎葉のエキス及びその乾燥粉末（以下、エキスの乾燥粉末のことを「エキス末」ともいう）などが挙げられる。 As the barley foliage of the specific variety used in the present invention, various processed products obtained from the foliage, that is, the processed barley of a specific variety can be used. Such processed products include, for example, dry powder of foliage, pulverized powder of foliage and dry powder thereof (hereinafter, the dry powder of pulverized material is also referred to as “pulverized powder”), fragmented foliage of foliage and dry Powders (hereinafter referred to as “dried powder”), squeezed leaves and leaves and dried powder thereof (hereinafter referred to as “squeezed powder”) And extract of stems and leaves and dry powder thereof (hereinafter, dry powder of extract is also referred to as “extract powder”).
大麦の茎葉を粉砕物及びその乾燥粉末化するには従来公知の方法を用いることができる。そのような方法としては、大麦の茎葉に対して、乾燥処理及び粉砕処理を組み合わせた方法を用いることができる。乾燥処理及び粉砕処理はいずれを先に行ってもよいが、乾燥処理を先に行うことが好ましい。乾燥粉末化は、この方法に、さらに必要に応じブランチング処理、殺菌処理などの処理から選ばれる１種又は２種以上の処理を組み合わせてもよい。また、粉砕処理を行う回数は１回でも、２回以上の処理を組合せてもよいが、粗粉砕処理を行った後に、より細かく粉砕する微粉砕処理を組合せることが好ましい。 Conventionally known methods can be used to pulverize barley foliage and pulverize it into dry powder. As such a method, a method combining drying treatment and pulverization treatment on barley foliage can be used. Either the drying process or the pulverization process may be performed first, but the drying process is preferably performed first. Dry powdering may be combined with one or more treatments selected from treatments such as blanching treatment and sterilization treatment, if necessary. In addition, the number of times of pulverization may be one time or a combination of two or more processes, but it is preferable to combine a fine pulverization process that finely pulverizes after the coarse pulverization process.
ブランチング処理とは、茎葉の緑色を鮮やかに保つための処理であり、ブランチング処理の方法としては、熱水処理や蒸煮処理などが挙げられる。 The blanching process is a process for keeping the green color of the foliage vivid, and examples of the blanching process include a hot water process and a steaming process.
熱水処理としては、例えば、８０〜１００℃、好ましくは９０〜１００℃の熱水又は水蒸気中で、大麦の茎葉を６０〜１８０秒間、好ましくは９０〜１２０秒間処理する方法などが挙げられる。また、熱水処理に際して、炭酸マグネシウムなどの炭酸塩や炭酸水素ナトリウムなどの炭酸水素塩を用いることが好ましく、炭酸水素の塩を熱水に溶解することにより、大麦の茎葉の緑色をより鮮やかにすることができる。 Examples of the hot water treatment include a method of treating barley foliage in hot water or steam at 80 to 100 ° C., preferably 90 to 100 ° C. for 60 to 180 seconds, preferably 90 to 120 seconds. Further, in the hot water treatment, it is preferable to use a carbonate such as magnesium carbonate or a hydrogen carbonate such as sodium hydrogen carbonate. By dissolving the hydrogen carbonate salt in hot water, the green color of the barley foliage is more vivid. can do.
蒸煮処理としては、常圧又は加圧下において、大麦の茎葉を水蒸気により蒸煮する処理と冷却する処理とを繰り返す間歇的蒸煮処理が好ましい。間歇的蒸煮処理において、水蒸気により蒸煮する処理は、例えば、２０〜４０秒間、好ましくは３０秒間行われる。蒸煮処理後の冷却処理は、直ちに行われることが好ましく、その方法は特に限定されないが、冷水への浸漬、冷蔵、冷風による冷却、温風による気化冷却、温風と冷風とを組み合わせた気化冷却などが用いられる。このうち温風と冷風とを組み合わせた気化冷却が好ましい。このような冷却処理は、大麦の茎葉の品温が、好ましくは６０℃以下、より好ましくは５０℃以下、さらに好ましくは４０℃以下となるように行われる。また、ビタミン、ミネラル、葉緑素などの栄養成分に富んだ大麦の茎葉の粉末を製造するためには、間歇的蒸煮処理を２〜５回繰り返すことが好ましい。 The steaming treatment is preferably an intermittent steaming treatment in which barley stems and leaves are steamed and steamed under normal pressure or pressure. In the intermittent steaming process, the steaming process is performed, for example, for 20 to 40 seconds, preferably 30 seconds. The cooling treatment after the steaming treatment is preferably performed immediately, and the method is not particularly limited, but it is immersed in cold water, refrigerated, cooling with cold air, evaporative cooling with hot air, evaporative cooling combining hot air and cold air Etc. are used. Among these, evaporative cooling combining hot air and cold air is preferable. Such cooling treatment is performed such that the barley foliage has an article temperature of preferably 60 ° C. or lower, more preferably 50 ° C. or lower, and even more preferably 40 ° C. or lower. In order to produce barley stalk and leaf powder rich in nutrients such as vitamins, minerals, and chlorophyll, it is preferable to repeat the intermittent steaming treatment 2 to 5 times.
殺菌処理は当業者に通常知られている処理であれば特に限定されないが、例えば、温度、圧力、電磁波、薬剤などを用いて物理的又は化学的に微生物を殺滅させる処理であるということができる。乾燥処理及び粉砕処理に追加してブランチング処理を行う場合、ブランチング処理は乾燥処理の前に行われることが好ましい。また乾燥処理及び粉砕処理に追加して殺菌処理を行う場合、殺菌処理は、乾燥処理の後か、粉砕処理の前又は後に行われることが好ましい。 The sterilization treatment is not particularly limited as long as it is a treatment generally known to those skilled in the art. For example, the sterilization treatment is a treatment that physically or chemically kills microorganisms using temperature, pressure, electromagnetic waves, chemicals, or the like. it can. When the branching process is performed in addition to the drying process and the pulverization process, the branching process is preferably performed before the drying process. Moreover, when performing a sterilization treatment in addition to the drying treatment and the pulverization treatment, the sterilization treatment is preferably performed after the drying treatment or before or after the pulverization treatment.
乾燥処理は特に限定されないが、例えば、大麦の茎葉の水分含量が１０％以下、好ましくは５％以下となるように乾燥する処理が挙げられる。乾燥処理は、例えば、熱風乾燥、高圧蒸気乾燥、電磁波乾燥、凍結乾燥などの当業者に公知の任意の方法により行われ得る。加熱による乾燥は、例えば、４０℃〜１４０℃、好ましくは８０℃〜１３０℃にて加温により茎葉が変色しない温度及び時間で行われ得る。 Although a drying process is not specifically limited, For example, the process dried so that the moisture content of barley foliage may be 10% or less, Preferably it is 5% or less is mentioned. The drying treatment can be performed by any method known to those skilled in the art, such as hot air drying, high pressure steam drying, electromagnetic wave drying, freeze drying, and the like. Drying by heating can be performed, for example, at a temperature and a time at which the foliage is not discolored by heating at 40 ° C to 140 ° C, preferably 80 ° C to 130 ° C.
粉砕処理は特に限定されないが、例えば、クラッシャー、ミル、ブレンダー、石臼などの粉砕用の機器や器具などを用いて、当業者が通常使用する任意の方法により植物体を粉砕する処理が挙げられる。粉砕された大麦の茎葉は、必要に応じて篩にかけられ、例えば、３０〜２５０メッシュを通過するものを大麦の茎葉の粉末として用いることが好ましい。粒径が２５０メッシュ通過のもの以下とすることで、さらなる加工時に大麦の茎葉の粉末が取り扱いやすくなり、粒径が３０メッシュ通過以上のものとすることで、大麦の茎葉の粉末と他の素材との均一な混合が容易になる。 The pulverization process is not particularly limited, and examples thereof include a process of pulverizing a plant body by an arbitrary method commonly used by those skilled in the art using a crusher, a mill, a blender, a millstone, or the like. The crushed barley foliage is sieved as necessary. For example, it is preferable to use a barley foliage powder that passes through 30 to 250 mesh. By making the particle size below 250 mesh, the barley foliage powder becomes easier to handle during further processing, and by making the particle size above 30 mesh, the barley foliage powder and other materials Uniform mixing with is easy.
具体的な粉砕末の製造方法としては、例えば、大麦の茎葉を切断した後、ブランチング処理を行い、次いで水分含量が１０質量％以下、好ましくは５質量％以下となるように乾燥し、その後粉砕する方法が挙げられる（特開２００４−０００２１０号公報を参照）。この他にも、例えば、大麦の茎葉を切断した後、ブランチング処理を行い、次いで揉捻し、その後、乾燥し、粉砕する方法（特開２００２−０６５２０４号公報、特許第３４２８９５６号公報を参照）；大麦の茎葉を乾燥し、粗粉砕した後、１１０℃以上で加熱し、さらに微粉砕する方法（特開２００３−０３３１５１号公報、特許第３２７７１８１号公報を参照）などが挙げられる。 As a specific method for producing a pulverized powder, for example, after cutting barley foliage, it is subjected to a blanching treatment, and then dried so that the water content is 10% by mass or less, preferably 5% by mass or less. The method of grind | pulverizing is mentioned (refer Unexamined-Japanese-Patent No. 2004-02010). In addition to this, for example, after cutting barley stalks and leaves, a branching treatment is performed, followed by twisting, and then drying and pulverization (see Japanese Patent Application Laid-Open No. 2002-0665204 and Japanese Patent No. 3428956). A method in which barley foliage is dried and coarsely pulverized, heated at 110 ° C. or higher, and further finely pulverized (see Japanese Patent Application Laid-Open Nos. 2003-033151 and 3277181).
大麦の茎葉を細片化する方法は特に限定されないが、例えば、スライス、破砕、細断などの当業者が植物体を細片化する際に通常使用する方法を用いることができる。細片化の一例として、スラリー化してもよい。スラリー化は、大麦の茎葉をミキサー、ジューサー、ブレンダー、マスコロイダーなどにかけ、大麦の茎葉をどろどろした粥状（液体と固体との懸濁液）にすることにより行う。このようにスラリー化することにより、茎葉は、細片の８０質量％以上が、好ましくは平均径１ｍｍ以下、より好ましくは０.５ｍｍ以下、さらに好ましくは０.１ｍｍ以下、なおさらに好ましくは０．０５ｍｍ以下となるように細片化され、流動性を有するようになる。細片化物は凍結乾燥や熱風乾燥などの処理を行い、乾燥粉末（細片化末）とすることもできる。 Although the method of fragmenting barley stalks and leaves is not particularly limited, for example, methods commonly used by those skilled in the art, such as slicing, crushing, and chopping, to fragment a plant can be used. As an example of fragmentation, a slurry may be used. Slurry is performed by putting barley stalks and leaves in a mixer, juicer, blender, mascolloider, etc., and forming barley stalks and leaves in a crumpled shape (suspension of liquid and solid). By slurrying in this way, 80% by mass or more of the foliage of the foliage is preferably 1 mm or less, more preferably 0.5 mm or less, even more preferably 0.1 mm or less, and still more preferably 0.8% or less. It is fragmented to be 05 mm or less, and has fluidity. The shredded product can be processed into freeze-dried powder (splintered powder) by subjecting it to freeze-drying or hot-air drying.
大麦の茎葉の搾汁液を得る方法は特に限定されないが、例えば、大麦の茎葉又はその細片化物を圧搾する方法、大麦の茎葉の細片化物を遠心やろ過する方法などを挙げることができる。具体的な搾汁液の製造方法の例としては、ミキサー、ジューサーなどの機械的破砕手段によって搾汁し、必要に応じて、篩別、濾過などの手段によって粗固形分を除去することにより搾汁液を得る方法が挙げられる。より具体的には、特開平０８−２４５４０８号公報、特開平０９−０４７２５２号公報、特開平５−７４７１号公報、特開平４−３４１１５３号公報などに記載の方法が挙げられ、これらの公知の方法を当業者が適宜選択して実施できる。搾汁液は、必要に応じて濃縮してもよいし、凍結乾燥や熱風乾燥、噴霧乾燥などの処理を行い、乾燥粉末（搾汁末）とすることもできる。 The method for obtaining the barley foliage juice is not particularly limited, and examples thereof include a method of squeezing barley foliage or fragments thereof, and a method of centrifuging or filtering barley foliage fragments. As a specific example of the method for producing the juice, the juice is squeezed by a mechanical crushing means such as a mixer or a juicer, and if necessary, the crude solid is removed by means such as sieving or filtration. The method of obtaining is mentioned. More specifically, the methods described in JP-A-08-245408, JP-A-09-047252, JP-A-5-7471, JP-A-4-341153 and the like can be mentioned. A person skilled in the art can appropriately select and carry out the method. The squeezed liquid may be concentrated as necessary, or may be processed into freeze-dried powder (squeezed powder) by lyophilization, hot air drying, spray drying, or the like.
大麦の茎葉のエキスを得る方法は特に限定されないが、例えば、大麦の茎葉又はその細片化物に、エタノール、水、含水エタノールなどの当業者が通常用いる抽出溶媒を加え、必要に応じて撹拌や加温して抽出する方法などを挙げることができる。抽出物は、必要に応じて濃縮してもよいし、凍結乾燥や熱風乾燥、噴霧乾燥などの処理を行い、乾燥粉末（エキス末）とすることもできる。 A method for obtaining an extract of barley foliage is not particularly limited. For example, an extraction solvent commonly used by those skilled in the art, such as ethanol, water, hydrous ethanol, or the like, is added to barley foliage or a fragmented product thereof. Examples of the method include extraction by heating. The extract may be concentrated as necessary, or may be processed into freeze powder (extract powder) by subjecting it to freeze drying, hot air drying, spray drying or the like.
大麦の茎葉の乾燥粉末の特性は特に限定されないが、例えば、その安息角について、２０°〜８０°が好ましく、３０°〜７０°がより好ましく、４０°〜６０°がさらに好ましく、４５°〜５５°がなおさらに好ましい。なお、安息角の測定方法は、安息角測定器（アズワン、ＡＳＫ−０１）を用いて、サンプル約５０ｇを高度１２ｃｍから半径４．３ｃｍのシャーレ中央に落下させ、次いで山型に堆積したサンプルの高さを測定し、次いでシャーレの半径及び堆積したサンプルの高さから下記式にて安息角を算出できる。
安息角＝ｔａｎ−１（ｂ／ａ）×１８０÷π（式中、ａ＝シャーレ半径、ｂ＝堆積したサンプルの高さを表わす。）
The characteristics of the dry powder of barley foliage are not particularly limited. For example, the angle of repose is preferably 20 ° to 80 °, more preferably 30 ° to 70 °, still more preferably 40 ° to 60 °, and more preferably 45 ° to Even more preferred is 55 °. The angle of repose is measured using an angle of repose measuring instrument (As One, ASK-01) by dropping about 50 g of sample into the center of a petri dish having an altitude of 12 cm to a radius of 4.3 cm, and then depositing the sample in a mountain shape. After measuring the height, the angle of repose can be calculated by the following formula from the radius of the petri dish and the height of the deposited sample.
Angle of repose = tan−1 (b / a) × 180 ÷ π (where, a = the petri dish radius, b = the height of the deposited sample)
大麦の茎葉の粉砕末の特性は特に限定されないが、例えば、メディアン径が１６μｍ以上であり、かつ、アスペクト比が２．０以下であり、前記メディアン径が２０μｍ以上であることが好ましい。特定の粒径とアスペクト比との組み合わせを有する大麦の茎葉の粉砕末を利用することにより、嗜好性に優れたものとできる。 Although the characteristics of the pulverized powder of barley foliage are not particularly limited, for example, the median diameter is preferably 16 μm or more, the aspect ratio is 2.0 or less, and the median diameter is preferably 20 μm or more. By using the pulverized powder of barley stalks and leaves having a specific particle size and aspect ratio combination, the palatability can be improved.
また、大麦の茎葉の粉砕末の別の特性として、例えばメディアン径が２０μｍ未満であり、かつ、ロジン・ラムラー式による分布定数ｎが１．６以上であり、前記メディアン径が１６μｍ未満であることが好ましい。特定の粒径とロジン・ラムラー式による分布定数ｎの組み合わせを有する大麦の茎葉の粉砕末を利用することにより、嗜好性に優れたものとできる。 Further, as another characteristic of the barley stalks and leaves, for example, the median diameter is less than 20 μm, the distribution constant n by the rosin-Rammler equation is 1.6 or more, and the median diameter is less than 16 μm. Is preferred. By using a pulverized powder of barley stalks and leaves having a specific particle size and a distribution constant n according to the Rosin-Rammler equation, the palatability can be improved.
ここで、ロジン・ラムラー式による分布定数ｎは、粒度分布に関するロジン・ラムラー（Ｒｏｓｉｎ−Ｒａｍｍｌｅｒ）式における定数ｎであり、粒度の均一性を表わす値である。 Here, the distribution constant n according to the Rosin-Rammler equation is a constant n in the Rosin-Ramler equation relating to the particle size distribution, and is a value representing the uniformity of the particle size.
ロジン・ラムラー式は、下記式（１）で表わされる。
Ｒ＝１００ｅｘｐ（−βＤｎ） （１）
式中、Ｄは粒径を表し、ＲはＤ（粒径）より大きな粒子の全粒子に対する百分率（％）を表わし、βは粒度特性係数を表わし、ｎは分布定数を表わす。
The Rosin-Rammler formula is represented by the following formula (1).
R = 100exp (−βDn) (1)
In the formula, D represents a particle size, R represents a percentage (%) of particles larger than D (particle size) to all particles, β represents a particle size characteristic coefficient, and n represents a distribution constant.
ここで、β＝１／Ｄｅｎとおくと、上記式（１）は
Ｒ＝１００ｅｘｐ｛−（Ｄ／Ｄｅ）ｎ ｝ （２）
のように書き換えられる。Ｄｅは粒度特性係数である。
Here, assuming that β = 1 / Den, the above formula (1) is R = 100exp {− (D / De) n} (2)
Can be rewritten as De is a particle size characteristic coefficient.
さらに、上記式（２）を書き換えると下記式（３）が得られる。
ｌｏｇ｛ｌｏｇ（１００／Ｒ）｝＝ｎｌｏｇＤ＋Ｃ （３）
ただし、Ｃ＝ｌｏｇ（ｌｏｇｅ）−ｎｌｏｇＤｅである。
Furthermore, when the above equation (2) is rewritten, the following equation (3) is obtained.
log {log (100 / R)} = nlogD + C (3)
However, C = log (log) -nlogDe.
上記式（３）から、ｘ軸にｌｏｇＤ、ｙ軸にｌｏｇ｛ｌｏｇ（１００／Ｒ）｝の目盛をつけたロジン・ラムラー（ＲＲ）線図にそれらの関係をプロットするとほぼ直線となる。その直線の勾配（分布定数ｎ）は粒度の均一性の度合いを表し、分布定数ｎの数値が大きいほど、粒子径範囲は狭く、粒子の大きさが揃っていることから、粒度の均一性に優れていると評価できる。 From the above equation (3), when the relationship is plotted on a Rosin-Rammler (RR) diagram with log D on the x-axis and log {log (100 / R)} on the y-axis, it becomes a substantially straight line. The slope of the straight line (distribution constant n) represents the degree of uniformity of particle size. The larger the value of distribution constant n, the narrower the particle size range and the uniform particle size. It can be evaluated as excellent.
本発明に用いられる特定品種の大麦の茎葉の乾燥粉末は、水不溶性食物繊維を含み得る。乾燥粉末に含まれる水不溶性食物繊維は、乾燥質量換算で２０質量％以上、好ましくは３０質量％以上含有することが好ましく、２０〜８０質量％、好ましくは３０〜７０質量％、より好ましくは３５〜６０質量％含有することがより好ましい。 The dry powder of barley foliage of a specific variety used in the present invention may contain water-insoluble dietary fiber. The water-insoluble dietary fiber contained in the dry powder is contained in an amount of 20% by mass or more, preferably 30% by mass or more, in terms of dry mass, preferably 20 to 80% by mass, preferably 30 to 70% by mass, more preferably 35%. It is more preferable to contain -60 mass%.
［２．乳酸菌増殖用組成物］
本発明は、大麦の茎葉の乾燥粉末を含有する乳酸菌増殖用組成物に関するものである。
[2. Composition for Lactic Acid Bacteria Growth]
The present invention relates to a composition for growing lactic acid bacteria containing a dry powder of barley foliage.
本発明の大麦の茎葉の乾燥粉末は、乳酸菌を培養し増殖させる作用を有する（以下「乳酸菌増殖用組成物」ともいう）。本発明の乳酸菌増殖用組成物に含有される大麦は、マンネンボシ、ダイシモチ、カシマムギ及びはるしずく（特定品種）から選ばれる少なくとも１の品種である。 The dry powder of barley foliage of the present invention has the effect of culturing and growing lactic acid bacteria (hereinafter also referred to as “composition for lactic acid bacteria growth”). The barley contained in the composition for growing lactic acid bacteria of the present invention is at least one variety selected from Mannenbosi, Daishimochi, Kashimumugi, and Harusu Shizuku (specific variety).
本発明においては、加工、貯蔵、運搬などの容易性や使用形態の汎用性といった観点から、大麦の茎葉の乾燥粉末、粉砕末、搾汁末、細片化末、エキス末であることが好ましく、特に、茎葉の粉砕末を用いることが、安価でかつ効率良く乳酸菌を増殖できるため、好ましい。 In the present invention, from the viewpoints of ease of processing, storage, transportation, etc. and versatility of usage forms, it is preferably a dry powder of barley foliage, pulverized powder, squeezed powder, shredded powder, and extract powder. In particular, it is preferable to use pulverized powder of stems and leaves because lactic acid bacteria can be propagated inexpensively and efficiently.
本発明の乳酸菌増殖用組成物は、特定品種の大麦の茎葉の乾燥粉末を含有することによって、乳酸菌を良好に増殖させることができる。乳酸菌増殖用組成物は、大麦の茎葉の乾燥粉末以外の成分として、オリゴ糖、水溶性食物繊維、ビタミン類、アミノ酸、乳酸菌等のその他の成分を含むことができる。 The composition for growing lactic acid bacteria of the present invention can grow lactic acid bacteria well by containing a dry powder of barley stalks and leaves of a specific variety. The composition for growing lactic acid bacteria can contain other components such as oligosaccharides, water-soluble dietary fibers, vitamins, amino acids, and lactic acid bacteria as components other than the dry powder of barley foliage.
本発明の乳酸菌増殖用組成物の固形分中、特定品種の大麦の茎葉の乾燥粉末の含有量は、下限値としては、０．１質量％以上が好ましく、０．５質量％以上がより好ましく、１質量％以上がさらに好ましく、１０質量％以上がなおさらに好ましく、２０質量％以上が特に好ましい。特定品種の大麦の茎葉の乾燥粉末の含有量が０．１質量％より少ない場合、本発明の効果が十分に発揮されない場合がある。本発明の乳酸菌増殖用組成物の一実施態様において、例えば、特定品種の大麦の茎葉の乾燥粉末の含有量は、例えば、その他の成分を併用する場合は全体量に対して、１０〜９０質量％であり、好ましくは２０〜８０質量％である。 In the solid content of the composition for growing lactic acid bacteria of the present invention, the content of the dry powder of barley foliage of a specific variety is preferably 0.1% by mass or more, more preferably 0.5% by mass or more as the lower limit. 1 mass% or more is further more preferable, 10 mass% or more is still more preferable, and 20 mass% or more is especially preferable. When the content of the dry powder of the barley foliage of a specific variety is less than 0.1% by mass, the effects of the present invention may not be sufficiently exhibited. In one embodiment of the composition for growing lactic acid bacteria of the present invention, for example, the content of dry powder of barley foliage of a specific variety is, for example, 10 to 90 mass relative to the total amount when other components are used in combination. %, Preferably 20 to 80% by mass.
乳酸菌としては、糖類から多量の乳酸を生成する細菌であれば、特に限定されず、目的に応じて適宜選択することができ、例えば、Bifidobacterium bifidum、Bifidobacterium breve、Bifidobacterium infantis、Bifidobacterium lactis、Bifidobacterium longum、Bifidobacterium adolescentis、Bifidobacterium mongoliense、Lactbacillus brevis、Lactbacillus gasseri、Lactobacillus acidophilus、Lactobacillus buchneri、Lactobacillus bulgaricus、Lactobacillus delburvecki、Lactobacillus casei、Lactobacillus crispatus、Lactobacillus curvatus、Lactobacillus halivaticus、Lactobacillus pentosus、Lactobacillus plantarum、Lactobacilus paracasei、Lactobacillus rhamnosus、Lactobacillus salivarius、Lactobacillus sporogenes、Lactobacillus sakei、Lactobacillus fructivorans、Lactobacillus hilgardii、Lactobacillus reuteri、Lactobacillus fermentum、Enterococcus faecalis(Streptococcus faecalis と称されることもある)、Enterococcus faesium（Streptococcus faesiumと称されることもある)、Streptococcus thermophilus、Lactococcus lactis(Streptococcus lactisと称されることもある)、Leuconostoc mesenteroides、Leuconostoc oenos、Pediococcus acidilactici、Pediococcus pentosaceus、Staphylococcus carnosus、Staphylococcus xylosus、Tetragenococcus halophilus、Bacillus coagulans、及びBacillus mesentericusなどが挙げられ、Bifidobacterium bifidum、Bifidobacterium breve、Bifidobacterium longum、Lactbacillus gasseri、Lactobacillus acidophilus、Lactobacillus casei、Lactobacillus plantarum、Lactobacilus paracasei、Lactobacillus plantarum、Lactobacillus fermentum、Lactobacilus paracasei、Enterococcus faecalis （Streptococcus faecalis)、Enterococcus faesium（Streptococcus faesium)、Streptococcus thermophilus、Lactococcus lactis(Streptococcus lactis)、Bacillus coagulans、Bacillus mesentericusが好ましい。これらは、１種を単独で使用してもよいし、２種以上を併用してもよい。乳酸菌増殖用組成物が乳酸菌を含有する場合、この乳酸菌は乳酸菌増殖用組成物が増殖対象とする乳酸菌と同一種のものであってもよく、異なる種のものであってもよい。 The lactic acid bacteria are not particularly limited as long as they produce a large amount of lactic acid from saccharides, and can be appropriately selected according to the purpose.For example, Bifidobacterium bifidum, Bifidobacterium breve, Bifidobacterium infantis, Bifidobacterium lactis, Bifidobacterium longum, Bifidobacterium adolescentis, Bifidobacterium mongoliense, Lactbacillus brevis, Lactbacillus gasseri, Lactobacillus acidophilus, Lactobacillus buchneri, Lactobacillus bulgaricus, Lactobacillus delburvecki, Lactobacillus casei, Lactobacillus crispatus, Lactobacillus curvatus, Lactobacillus halivaticus, Lactobacillus pentosus, Lactobacillus plantarum, Lactobacilus paracasei, Lactobacillus rhamnosus, Lactobacillus salivarius , Lactobacillus sporogenes, Lactobacillus sakei, Lactobacillus fructivorans, Lactobacillus hilgardii, Lactobacillus reuteri, Lactobacillus fermentum, Enterococcus faecalis (sometimes called Streptococcus faecalis), Enterococcus faesiu m (sometimes called Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (sometimes called Streptococcus lactis), Leuconostoc mesenteroides, Leuconostoc oenos, Pediococcus acidilactici, Pediococcus pentoscusus, carnosocus , Bacillus coagulans, Bacillus mesentericus, etc. ), Enterococcus faesium (Streptococcus faesium), Streptococcus thermophilus, Lactococcus lactis (Streptococcus lactis), Bacillus coagulans, Bacillus mesentericus. These may be used individually by 1 type and may use 2 or more types together. When the composition for lactic acid bacteria growth contains lactic acid bacteria, the lactic acid bacteria may be of the same type as the lactic acid bacteria to be propagated by the composition for lactic acid bacteria growth, or may be of a different type.
乳酸菌の性質は特に限定されず、目的に応じて適宜選択することができ、例えば、耐熱性、耐酸性、耐糖性、耐塩性、有胞子性などが挙げられる。 The properties of lactic acid bacteria are not particularly limited and can be appropriately selected depending on the purpose. Examples thereof include heat resistance, acid resistance, sugar resistance, salt resistance, and spore resistance.
乳酸菌の入手方法は特に限定されず、目的に応じて適宜選択することができ、例えば、ヨーグルトや野菜などの食品から単離された乳酸菌や市販品を用いてもよい。 The method for obtaining lactic acid bacteria is not particularly limited, and can be appropriately selected according to the purpose. For example, lactic acid bacteria isolated from foods such as yogurt and vegetables or commercially available products may be used.
例えば、乳酸菌増殖用組成物を、後述するように乳酸菌用培地及び乳酸菌の培養方法に用いる場合は、無機塩類を含む溶媒に、固体の形態の乳酸菌増殖用組成物を分散又は溶解して分散液又は溶解液などの液体の形態の乳酸菌増殖用組成物を調製する方法などが挙げられる。この場合、任意成分を溶媒に分散又は溶解するタイミングは、大麦の茎葉の加工物の溶媒への分散又は溶解と同時である必要はなく、大麦の茎葉の加工物を分散又は溶解した前、又は後のいずれであってもよい。 For example, when the composition for growing lactic acid bacteria is used in a culture medium for lactic acid bacteria and a method for cultivating lactic acid bacteria as will be described later, the composition for growing lactic acid bacteria in a solid form is dispersed or dissolved in a solvent containing inorganic salts. Alternatively, a method of preparing a composition for growing lactic acid bacteria in the form of a liquid such as a lysis solution may be mentioned. In this case, the timing of dispersing or dissolving the optional components in the solvent need not be the same as the dispersion or dissolution of the barley foliage processed product in the solvent, or before the barley foliage processed product is dispersed or dissolved, or Any of later may be sufficient.
無機塩類を含む溶媒は特に限定されず、目的に応じて適宜選択することができ、例えば、生理食塩水、リン酸緩衝液、リン酸緩衝生理食塩水などが挙げられるが、これらの中でも、リン酸緩衝生理食塩水（以下「ＰＢＳ」ともいう。）が好ましい。このような乳酸菌増殖用組成物は、以下に示す、乳酸菌用培地及び乳酸菌の培養方法において好適に用いることができる。 The solvent containing the inorganic salt is not particularly limited and can be appropriately selected depending on the purpose. Examples thereof include physiological saline, phosphate buffer, phosphate buffered saline, etc. Acid buffered saline (hereinafter also referred to as “PBS”) is preferred. Such a composition for growing lactic acid bacteria can be suitably used in the following culture medium for lactic acid bacteria and culturing method for lactic acid bacteria.
（乳酸菌用培地及び乳酸菌の培養方法）
乳酸菌用培地は、乳酸菌増殖用組成物を含有してなり、さらに必要に応じて乳酸菌の生育に好適な成分を含有してなる。乳酸菌の培養方法は、乳酸菌用培地を用いて乳酸菌を培養する方法である。
(Medium for lactic acid bacteria and culture method of lactic acid bacteria)
The medium for lactic acid bacteria contains a composition for growing lactic acid bacteria, and further contains components suitable for the growth of lactic acid bacteria as necessary. The method for culturing lactic acid bacteria is a method for culturing lactic acid bacteria using a medium for lactic acid bacteria.
乳酸菌増殖用組成物及び乳酸菌用培地により増殖される乳酸菌としては、糖類から多量の乳酸を生成する細菌であれば、特に限定されず、目的に応じて適宜選択することができ、上記の乳酸菌などが挙げられる。 The lactic acid bacteria grown on the lactic acid bacteria growth composition and the lactic acid bacteria culture medium are not particularly limited as long as they are bacteria that produce a large amount of lactic acid from saccharides, and can be appropriately selected according to the purpose. Is mentioned.
乳酸菌用培地における培地成分としては、乳酸菌増殖用組成物を含有するものであれば、特に限定されず、通常乳酸菌に使用される培地の成分の中から、目的に応じて適宜選択することができ、例えば、グルコース、オリゴ糖などの炭素源；ポリペプトン、酵母エキス、カゼインなどの窒素源；リン酸ナトリウム、リン酸カリウム、塩化ナトリウム、炭酸カルシウム、硫酸アンモニウムなどの無機塩類やリン酸緩衝生理食塩水などの乳酸菌の生育に好適な成分などが挙げられるが、リン酸緩衝生理食塩水とマンネンボシ、ダイシモチ、カシマムギ及びはるしずくから選ばれる少なくとも１の特定品種の大麦の茎葉の乾燥粉末を含有する乳酸菌増殖用組成物とからなる液体培地が、好適に乳酸菌を増殖させることができる点で好ましい。また、乳酸菌増殖用組成物を含む液体培地を、前培養において用いてもよく、本培養において用いてもよい。 The medium component in the medium for lactic acid bacteria is not particularly limited as long as it contains a composition for growing lactic acid bacteria, and can be appropriately selected from the components of the medium normally used for lactic acid bacteria according to the purpose. For example, carbon sources such as glucose and oligosaccharides; nitrogen sources such as polypeptone, yeast extract and casein; inorganic salts such as sodium phosphate, potassium phosphate, sodium chloride, calcium carbonate, ammonium sulfate, and phosphate buffered saline Ingredients suitable for the growth of lactic acid bacteria include, but are not limited to, a phosphate buffered saline solution and a lactic acid bacteria growth containing a dry powder of barley stems and leaves of at least one specific variety selected from Mannenbosi, Daishimochi, Kashimumugi and Harushizuku A liquid medium comprising the composition for use is preferable in that lactic acid bacteria can be suitably grown. Moreover, the liquid culture medium containing the composition for lactic acid bacteria growth may be used in preculture, and may be used in main culture.
乳酸菌用培地における特定品種の大麦の茎葉の乾燥粉末の含有量は特に限定されず、目的に応じて適宜選択することができるが、下限値としては、０．００２質量％以上が好ましく、０．００３質量％以上がより好ましく、０．０１質量％以上がさらに好ましく、０．１質量％以上がなおさらに好ましく、上限値としては、９０質量％以下が好ましい。特定品種の大麦の茎葉の乾燥粉末の含有量が０．００２質量％未満であると、乳酸菌が増殖されにくいことがある。 The content of the dry powder of barley foliage of a specific variety in the medium for lactic acid bacteria is not particularly limited and can be appropriately selected according to the purpose. However, the lower limit is preferably 0.002% by mass or more. 003% by mass or more is more preferable, 0.01% by mass or more is more preferable, 0.1% by mass or more is still more preferable, and the upper limit is preferably 90% by mass or less. If the content of the dry powder of the barley foliage of a specific variety is less than 0.002% by mass, lactic acid bacteria may be difficult to grow.
乳酸菌用培地における滅菌条件としては、特定品種の大麦の茎葉の乾燥粉末に変質が生じず、乳酸菌に用いることができる培地を滅菌できる条件であれば、特に限定されず、目的に応じて適宜選択することができるが、１１０℃〜１３０℃、１０分間〜３０分間で高圧蒸気滅菌することが好ましい。 The sterilization conditions in the medium for lactic acid bacteria are not particularly limited as long as the conditions do not cause alteration in the dry powder of barley stalks and leaves of a particular variety and can be used for lactic acid bacteria, and are appropriately selected depending on the purpose. However, it is preferable to perform autoclaving at 110 to 130 ° C. for 10 to 30 minutes.
乳酸菌の培養条件（培地中のｐＨ、溶存酸素、培養温度、培養時間など）としては、通常乳酸菌に使用される培養条件であれば、特に限定されず、目的に応じて適宜選択することができ、例えば、乳酸菌の科学と技術（乳酸菌研究集談会 編）などに記載の培養条件などが挙げられる。 The culture conditions for lactic acid bacteria (pH in the medium, dissolved oxygen, culture temperature, culture time, etc.) are not particularly limited as long as they are culture conditions normally used for lactic acid bacteria, and can be appropriately selected according to the purpose. Examples thereof include culture conditions described in the science and technology of lactic acid bacteria (edited by lactic acid bacteria research conference).
本発明の飲食用組成物を用いた乳酸菌増殖用組成物は、生体内において乳酸菌を増殖させるものであり得る。乳酸菌増殖用組成物は、生体外において乳酸菌を増殖促進させるものであってもよい。 The composition for growing lactic acid bacteria using the composition for eating and drinking according to the present invention can grow lactic acid bacteria in vivo. The composition for growing lactic acid bacteria may promote the growth of lactic acid bacteria in vitro.
乳酸菌増殖用組成物は、これを用いることにより、安価かつ簡便に、乳酸菌を増殖させることができ、しかも従来の大麦品種の茎葉を用いた乳酸菌増殖用組成物と比較して、乳酸菌の増殖促進効果が高いものとなるので、例えば、乳酸菌を含有する素材、食品、食品素材、食品組成物などの添加物として好適に利用することができる。 By using this composition for growing lactic acid bacteria, it is possible to grow lactic acid bacteria inexpensively and easily, and also, compared with the conventional composition for growing lactic acid bacteria using barley varieties, the growth of lactic acid bacteria is promoted. Since the effect is high, for example, it can be suitably used as an additive such as a material containing lactic acid bacteria, a food, a food material, or a food composition.
同様に、乳酸菌増殖用組成物を含有する乳酸菌用培地は、これを用いることにより、安価かつ簡便に、乳酸菌を増殖させることができ、しかも従来の大麦品種の茎葉を用いた乳酸菌増殖用培地と比較して、乳酸菌の増殖促進効果が高いものとなるので、乳酸菌の培養に好適に用いることができ、安全性にも優れるため、例えば、乳酸菌を含有する素材、食品、食品素材、食品組成物などの添加物として好適に利用することができる。 Similarly, the medium for lactic acid bacteria containing the composition for growing lactic acid bacteria can be used to grow lactic acid bacteria inexpensively and easily, and further, a medium for growing lactic acid bacteria using the barley varieties of conventional barley varieties. In comparison, since the effect of promoting the growth of lactic acid bacteria is high, it can be suitably used for culturing lactic acid bacteria and is excellent in safety. For example, materials, foods, food materials, food compositions containing lactic acid bacteria It can utilize suitably as additives, such as.
以下、本発明を実施例によりさらに詳細に説明するが、本発明はこれら実施例に限定されるものではなく、本発明の課題を解決し得る限り、本発明は種々の態様をとることができる。 Hereinafter, the present invention will be described in more detail with reference to examples. However, the present invention is not limited to these examples, and the present invention can take various modes as long as the problems of the present invention can be solved. .
［製造例］
製造例１．大麦茎葉の粉砕末試料
原料として、出穂前に刈り取ったニシノホシの茎葉を用いた。これを水洗いし、付着した泥などを除去し、５〜１０ｃｍ程度の大きさに切断する前処理を行った。前処理した茎葉を、９０〜１００℃の熱湯で９０秒間〜１２０秒間、１回のみブランチング処理し、その後、冷水で冷却した。続いて、得られた茎葉を、水分量が５質量％以下となるまで、乾燥機中で、２０分間〜１８０分間、８０℃〜１３０℃の温風にて乾燥させた。乾燥した茎葉を約１ｍｍの大きさに粉砕処理した。得られた大麦の茎葉を、２００メッシュ区分を９０％以上が通過するように粉砕処理し、ニシノホシ茎葉の粉砕末試料を得た。
[Production example]
Production Example 1 Nishinohoshi foliage cut before heading was used as a raw material for pulverized barley foliage. This was washed with water to remove adhering mud and the like, and a pretreatment was performed to cut it to a size of about 5 to 10 cm. The pretreated stalks and leaves were blanched only once with hot water at 90 to 100 ° C. for 90 to 120 seconds, and then cooled with cold water. Subsequently, the obtained foliage was dried with warm air at 80 ° C. to 130 ° C. for 20 minutes to 180 minutes in a dryer until the water content became 5% by mass or less. The dried foliage was pulverized to a size of about 1 mm. The obtained barley stalks and leaves were pulverized so that 90% or more passed through the 200 mesh section, and a crushed powder sample of Nishinohoshi stalks and leaves was obtained.
ニシノホシの代わりに、マンネンボシ、はるしずく、ダイシモチ、カシマムギ及びシュンライを用いた以外は、ニシノホシ茎葉の粉砕末試料を得たのと同様にして、各大麦品種茎葉の粉砕末試料を得た。 A ground powder sample of each barley variety stem and leaf was obtained in the same manner as the ground powder sample of Nishinohoshi stem and leaf except that Mannenbosi, Harushizuku, Daishimochi, Kashimamugi and Shunrai were used instead of Nishinohoshi.
［実施例１．乳酸菌増殖評価］
製造例１に記載の方法にてマンネンボシ、はるしずく、ダイシモチ、カシマムギ、ニシノホシ及びシュンライの茎葉の粉砕末試料を得、得られた各大麦の粉砕末試料について、生体内での乳酸菌増殖効果のモデル試験として、以下の乳酸菌増殖試験を実施した。本発明の実施例として、マンネンボシ、はるしずく、ダイシモチ及びカシマムギを用い、比較例としてニシノホシ及びシュンライを用いた。
[Example 1. Lactic acid bacteria growth evaluation]
By the method described in Production Example 1, pulverized powder samples of stalks and leaves of Mannenbosi, Harushizuku, Daishimochi, Kishimugi, Nishinohoshi and Shunrai are obtained, and the obtained pulverized powder samples of each barley have an effect of growing lactic acid bacteria in vivo. The following lactic acid bacteria growth test was performed as a model test. As an example of the present invention, Mannenboshi, Harushizuku, Daishimochi and Kashimumugi were used, and Nishinohoshi and Shunrai were used as comparative examples.
（乳酸菌用培地の作成）
各粉末試料０．１ｇをそれぞれ試験管に量りとり、食品衛生検査指針に準じて作製したリン酸緩衝生理食塩水（ＰＢＳ）１０ｍＬで懸濁させた。これをオートクレーブにて１２１℃、２０ｍｉｎの条件で滅菌し、乳酸菌用培地を得た。
(Creation of medium for lactic acid bacteria)
0.1 g of each powder sample was weighed into a test tube and suspended in 10 mL of phosphate buffered saline (PBS) prepared according to the food hygiene inspection guidelines. This was sterilized in an autoclave at 121 ° C. for 20 minutes to obtain a medium for lactic acid bacteria.
（乳酸菌の培養）
乳酸菌として、ＣＥＬＬ ＢＩＯＴＥＣＨ製 Enterococcus faecalis（Streptococcus faecalis）の乾燥菌体（白色微粉末） １×１０11個／ｇを使用した。この乾燥菌体約１０ｍｇを試験管にとり、ＭＲＳ Ｂｒｏｔｈ（ＭＥＲＣＫ社製）１０ｍＬで懸濁させ、３７℃で約１８時間培養した。得られた懸濁液を１００００倍まで段階希釈したもの０．１ｍＬを、前記で得られた各乳酸菌用培地１０ｍＬに添加し、３７℃、４時間の条件で静置培養を行った。これらの培養液について、培養開始より０、４時間時点における菌数測定（ＣＦＵ／ｍＬ）を行った。ＣＦＵ測定は、具体的には、ＭＲＳ Ａｇｅｒ（ＭＥＲＣＫ社製）の寒天プレートに、適宜希釈した各培養時点の培養液１００μｌを付したものを、３５℃で２４時間インキュベートした後、コロニー数を計数することによって行った。菌数測定値（ＣＦＵ／ｍＬ）を、乳酸菌数として下記の表１に示す。
(Cultivation of lactic acid bacteria)
As the lactic acid bacteria, 1 × 10 11 cells / g of dry cells (white fine powder) of Enterococcus faecalis (Streptococcus faecalis) manufactured by CELL BIOTECH were used. About 10 mg of the dried cells were taken in a test tube, suspended in 10 mL of MRS Broth (MERCK), and cultured at 37 ° C. for about 18 hours. 0.1 mL of the suspension obtained by serial dilution up to 10,000 times was added to 10 mL of each lactic acid bacteria medium obtained above, and static culture was performed at 37 ° C. for 4 hours. For these cultures, the number of bacteria (CFU / mL) was measured at 0 and 4 hours from the start of culture. Specifically, the CFU measurement was performed by incubating 100 μl of an appropriately diluted culture solution at each culture point on an agar plate of MRS Ager (manufactured by MERCK) for 24 hours at 35 ° C., and then counting the number of colonies. Went by. The measured number of bacteria (CFU / mL) is shown in Table 1 below as the number of lactic acid bacteria.
表１に示す結果から、比較例であるニシノホシ及びシュンライの茎葉の粉砕末と比較して、本願発明の実施例であるマンネンボシ、はるしずく、ダイシモチ及びカシマムギの茎葉の粉砕末を用いて乳酸菌を培養すると、乳酸菌が良好に増殖することがわかった。特に、乳酸菌増殖効果が知られている大麦の品種であるシュンライと比較して、本発明の実施例であるマンネンボシ、はるしずく、ダイシモチ及びカシマムギは１．７〜６．３倍、増加しており、これらの大麦の茎葉から得られる粉砕末は、優れた乳酸菌増殖能を有することがわかった。 From the results shown in Table 1, compared with the ground powder of Nishinohoshi and Shunrai, which are comparative examples, the lactic acid bacteria were pulverized using the ground powder of Mannenbosi, Harushizuku, Daishimochi, and Koshimugi, which are examples of the present invention. It was found that lactic acid bacteria proliferate well when cultured. In particular, compared to Shunrai, which is a barley variety known to have a lactic acid bacteria growth effect, Mannenbosi, Harushizuku, Daishimochi, and Koshimagi, which are examples of the present invention, are increased by 1.7 to 6.3 times. It was found that the pulverized powder obtained from the barley foliage had an excellent ability to grow lactic acid bacteria.
本発明によれば、安価かつ簡便に乳酸菌を増殖させることができる乳酸菌増殖促進組成物を提供することができる。 According to the present invention, it is possible to provide a composition for promoting the growth of lactic acid bacteria capable of growing lactic acid bacteria easily and inexpensively.
Claims (4)
前記大麦が、マンネンボシ、ダイシモチ、カシマムギ及びはるしずくから選ばれる少なくとも１の品種の大麦であり、前記乾燥粉末の安息角が２０°〜８０°である、乳酸菌増殖用組成物。 A composition for growing lactic acid bacteria, comprising a dry powder of barley stems and / or leaves,
A composition for growing lactic acid bacteria, wherein the barley is at least one variety of barley selected from mannenbosi, daisimochi, oak and wheat, and the repose angle of the dry powder is 20 ° to 80 °.
前記粉砕末のメディアン径が１６μｍ以上であり、かつ、アスペクト比が２．０以下である、請求項１又は２のいずれかに記載の乳酸菌増殖用組成物。 The dry powder is pulverized powder,
The composition for growing lactic acid bacteria according to claim 1 or 2, wherein a median diameter of the pulverized powder is 16 µm or more and an aspect ratio is 2.0 or less.
前記粉砕末のメディアン径が２０μｍ未満であり、かつ、ロジン・ラムラー式による分布定数ｎが１．６以上である、請求項１又は２のいずれかに記載の乳酸菌増殖用組成物。 The dry powder is pulverized powder,
The composition for growing lactic acid bacteria according to claim 1, wherein the median diameter of the pulverized powder is less than 20 μm, and the distribution constant n according to the rosin-Rammler equation is 1.6 or more.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015133930A JP5866744B1 (en) | 2015-02-19 | 2015-07-02 | Composition for growing lactic acid bacteria |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2015030992 | 2015-02-19 | ||
| JP2015030992 | 2015-02-19 | ||
| JP2015133930A JP5866744B1 (en) | 2015-02-19 | 2015-07-02 | Composition for growing lactic acid bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP5866744B1 JP5866744B1 (en) | 2016-02-17 |
| JP2016154526A true JP2016154526A (en) | 2016-09-01 |
Family
ID=55347071
Family Applications (5)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015133931A Active JP5887470B1 (en) | 2015-02-19 | 2015-07-02 | Barley stems and / or leaves from Aso |
| JP2015133930A Active JP5866744B1 (en) | 2015-02-19 | 2015-07-02 | Composition for growing lactic acid bacteria |
| JP2015134812A Active JP5887471B1 (en) | 2015-02-19 | 2015-07-03 | Eating and drinking composition |
| JP2015134813A Active JP5906511B1 (en) | 2015-02-19 | 2015-07-03 | Eating and drinking composition |
| JP2016005712A Active JP5986327B2 (en) | 2015-02-19 | 2016-01-15 | Composition for eating and drinking, composition for growing lactic acid bacteria, stem and / or leaf of barley from Aso, barley stem and / or leaf cultivated using black mycelium |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015133931A Active JP5887470B1 (en) | 2015-02-19 | 2015-07-02 | Barley stems and / or leaves from Aso |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015134812A Active JP5887471B1 (en) | 2015-02-19 | 2015-07-03 | Eating and drinking composition |
| JP2015134813A Active JP5906511B1 (en) | 2015-02-19 | 2015-07-03 | Eating and drinking composition |
| JP2016005712A Active JP5986327B2 (en) | 2015-02-19 | 2016-01-15 | Composition for eating and drinking, composition for growing lactic acid bacteria, stem and / or leaf of barley from Aso, barley stem and / or leaf cultivated using black mycelium |
Country Status (1)
| Country | Link |
|---|---|
| JP (5) | JP5887470B1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6664929B2 (en) * | 2015-10-30 | 2020-03-13 | キリンビバレッジ株式会社 | Packaged beverages containing indigestible dextrin |
| JP6089165B1 (en) * | 2016-02-25 | 2017-03-08 | 株式会社東洋新薬 | Bifidobacterium composition |
| JP2018042504A (en) * | 2016-09-14 | 2018-03-22 | 株式会社東洋新薬 | Green leaf processed product |
| JP7321480B2 (en) * | 2016-12-15 | 2023-08-07 | 株式会社東洋新薬 | Composition |
| JP6330121B1 (en) * | 2017-03-31 | 2018-05-30 | 株式会社東洋新薬 | Eating and drinking composition |
| JP6296525B1 (en) * | 2017-05-17 | 2018-03-20 | 株式会社萌芽プランツ | Nijo barley young leaf dried product for green juice |
| JP7046302B2 (en) * | 2018-03-29 | 2022-04-04 | 株式会社センリファーム | Drying method and drying equipment for green juice raw materials |
| JP2019180392A (en) * | 2018-03-30 | 2019-10-24 | 株式会社東洋新薬 | Drinking composition for green juice |
| JP6738469B2 (en) * | 2018-07-31 | 2020-08-12 | 株式会社 伊藤園 | Method for producing young barley juice |
| JP6671073B2 (en) * | 2019-06-07 | 2020-03-25 | 株式会社 伊藤園 | Production method of barley young leaf juice |
| JP7211991B2 (en) * | 2020-02-28 | 2023-01-24 | 株式会社 伊藤園 | Method for producing green barley juice |
| JP6945888B1 (en) * | 2020-09-30 | 2021-10-06 | 株式会社東洋新薬 | Composition for improving immune function |
| WO2022209485A1 (en) | 2021-03-29 | 2022-10-06 | デンカ株式会社 | Agent for increasing expression of gene and photosynthesis activating agent |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6115726A (en) * | 1984-06-29 | 1986-01-23 | Shin Nippon Kagaku Kogyo Co Ltd | Method for preventing caking of particulate material |
| JP2008146590A (en) * | 2006-12-13 | 2008-06-26 | Dish Fine:Kk | Image print bookbinding system |
| JP2013031426A (en) * | 2011-06-28 | 2013-02-14 | Toyo Shinyaku Co Ltd | Composition for proliferating lactobacillus, medium for lactobacillus and method for culturing lactobacillus |
| JP2014060948A (en) * | 2012-09-20 | 2014-04-10 | Niigata Univ | Method for determining kinds of beer raw materials and products, and method for evaluating beer quality using the method |
| WO2014136874A1 (en) * | 2013-03-07 | 2014-09-12 | 大塚製薬株式会社 | Food containing barley powder |
| JP2015006171A (en) * | 2013-05-29 | 2015-01-15 | 株式会社東洋新薬 | Composition for lactobacillus multiplication |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4183886B2 (en) * | 2000-05-02 | 2008-11-19 | 株式会社東洋新薬 | Antihypertensive food containing raw material derived from wheat |
| JP3706628B1 (en) * | 2004-07-07 | 2005-10-12 | 株式会社東洋新薬 | Granulated products containing processed wheat leaves |
| JP5337027B2 (en) * | 2007-05-28 | 2013-11-06 | 三菱商事フードテック株式会社 | Spherical mannitol crystal particles |
| JP5131882B1 (en) * | 2012-06-26 | 2013-01-30 | 紀子 石居 | Low sugar food material, fermented dough, bread, confectionery and noodles using low sugar food material |
| WO2014038497A1 (en) * | 2012-09-05 | 2014-03-13 | 株式会社ヤクルト本社 | Noodles containing green leaves of gramineous plant and oligosaccharide |
| JP2015126723A (en) * | 2013-05-29 | 2015-07-09 | 株式会社東洋新薬 | Edible composition, method for improving taste and color of edible composition, composition for growth of lactic acid bacterium, culture method of foliage of barley, and foliage of barley cultured by using andosols or aso-grown soil |
| JP5363674B1 (en) * | 2013-05-29 | 2013-12-11 | 株式会社東洋新薬 | Eating and drinking composition for green juice |
| JP6463083B2 (en) * | 2013-10-31 | 2019-01-30 | 株式会社東洋新薬 | Barley leaves and / or stems cultivated with black soil, barley leaves and / or stems from Aso, food and beverage compositions containing them, and methods for cultivating barley stems and / or leaves |
| JP5681897B1 (en) * | 2013-10-31 | 2015-03-11 | 株式会社東洋新薬 | Aso barley leaves and / or stems and food and drink compositions containing them |
| JP5961238B2 (en) * | 2013-10-31 | 2016-08-02 | 株式会社東洋新薬 | Eating and drinking composition with improved flavor |
-
2015
- 2015-07-02 JP JP2015133931A patent/JP5887470B1/en active Active
- 2015-07-02 JP JP2015133930A patent/JP5866744B1/en active Active
- 2015-07-03 JP JP2015134812A patent/JP5887471B1/en active Active
- 2015-07-03 JP JP2015134813A patent/JP5906511B1/en active Active
-
2016
- 2016-01-15 JP JP2016005712A patent/JP5986327B2/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6115726A (en) * | 1984-06-29 | 1986-01-23 | Shin Nippon Kagaku Kogyo Co Ltd | Method for preventing caking of particulate material |
| JP2008146590A (en) * | 2006-12-13 | 2008-06-26 | Dish Fine:Kk | Image print bookbinding system |
| JP2013031426A (en) * | 2011-06-28 | 2013-02-14 | Toyo Shinyaku Co Ltd | Composition for proliferating lactobacillus, medium for lactobacillus and method for culturing lactobacillus |
| JP2014060948A (en) * | 2012-09-20 | 2014-04-10 | Niigata Univ | Method for determining kinds of beer raw materials and products, and method for evaluating beer quality using the method |
| WO2014136874A1 (en) * | 2013-03-07 | 2014-09-12 | 大塚製薬株式会社 | Food containing barley powder |
| JP2015006171A (en) * | 2013-05-29 | 2015-01-15 | 株式会社東洋新薬 | Composition for lactobacillus multiplication |
Also Published As
| Publication number | Publication date |
|---|---|
| JP5986327B2 (en) | 2016-09-06 |
| JP2016155796A (en) | 2016-09-01 |
| JP5906511B1 (en) | 2016-04-20 |
| JP5866744B1 (en) | 2016-02-17 |
| JP5887470B1 (en) | 2016-03-16 |
| JP2016154528A (en) | 2016-09-01 |
| JP2016154527A (en) | 2016-09-01 |
| JP5887471B1 (en) | 2016-03-16 |
| JP2016154533A (en) | 2016-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5866744B1 (en) | Composition for growing lactic acid bacteria | |
| JP5682082B2 (en) | Composition for growing lactic acid bacteria | |
| KR101759149B1 (en) | Powdery malted rice extract composition | |
| KR101780176B1 (en) | Lactic acid bacterium proliferation promoter | |
| JP5544665B2 (en) | Composition for growing lactic acid bacteria, medium for lactic acid bacteria, and method for culturing lactic acid bacteria | |
| JP2017006113A (en) | Edible composition, lactic acid bacteria propagation composition, stems and/or leaves of barley from aso, and stems and/or leaves of barley cultivated using andosols | |
| CN104651275A (en) | Probiotic culture medium and application thereof | |
| JP2000032909A (en) | Food for improving digestion and absorption by environmental improvement caused by enteric microbe | |
| KR101193338B1 (en) | Food composition comprising lactic acid bacteria fermented sweet potato and preparation method thereof | |
| JP7149011B2 (en) | Antiallergic agent, intestinal immunity enhancer, intestinal adhesion improver for lactic acid bacteria | |
| KR100574413B1 (en) | Process for preparing lactic acid fermented milk from vegetable matter | |
| JP6089165B1 (en) | Bifidobacterium composition | |
| JP3627968B2 (en) | Lactic acid fermented food and method for producing the same | |
| KR102282725B1 (en) | Weissella cibaria strain having rice cake anti-retrogradation activity and use thereof | |
| JP2005006540A (en) | Fermentation promotor and fermented milk | |
| KR101822425B1 (en) | Method of manufacturing bean yogurt using fermented black garlic | |
| KR101822420B1 (en) | Method of manufacturing bean yogurt using fermented red ginseng | |
| JP2015167500A (en) | Health food containing fermented watermelon seed | |
| JP7382654B2 (en) | Composition | |
| KR102080324B1 (en) | Rice yogurts and manufacturing method thereof | |
| Sulabo et al. | Storage stability of freeze-dried Lactobacillus plantarum S20 starter culture as affected by various formulations of drying medium, and its fermentation characteristics in mung bean (Vigna radiata L.) slurry | |
| JP6484302B2 (en) | Oral composition | |
| Nawel et al. | Kinetic study of the growth of Lactobacillus bulgaricus and Streptococcus thermophilus from date syrup variety Hmira and carob pods syrup | |
| Minh et al. | Several technical factors affecting the baby corn beverage production |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20151116 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20151207 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20151218 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5866744 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |