JP2015530074A - 生物学的マイクロ物体の結合 - Google Patents

生物学的マイクロ物体の結合 Download PDF

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Publication number
JP2015530074A
JP2015530074A JP2015521839A JP2015521839A JP2015530074A JP 2015530074 A JP2015530074 A JP 2015530074A JP 2015521839 A JP2015521839 A JP 2015521839A JP 2015521839 A JP2015521839 A JP 2015521839A JP 2015530074 A JP2015530074 A JP 2015530074A
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micro
objects
channel
biological
micro object
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JP2015521839A
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English (en)
Japanese (ja)
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JP2015530074A5 (enExample
Inventor
ウー，ミン，シー．
チャップマン，ケヴィン，ティー．
カンドロス，イゴール，ワイ．
マチュー，ガエタン，エル．
ショート，スティーブン，ダブリュー．
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バークレー ライツ，インコーポレイテッド
バークレー ライツ，インコーポレイテッド
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Publication of JP2015530074A publication Critical patent/JP2015530074A/ja
Publication of JP2015530074A5 publication Critical patent/JP2015530074A5/ja
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0454—Moving fluids with specific forces or mechanical means specific forces radiation pressure, optical tweezers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Fluid Mechanics (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2015521839A 2012-07-13 2013-07-12 生物学的マイクロ物体の結合 Pending JP2015530074A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261671499P 2012-07-13 2012-07-13
US61/671,499 2012-07-13
US201261720956P 2012-10-31 2012-10-31
US61/720,956 2012-10-31
PCT/US2013/050269 WO2014011985A1 (en) 2012-07-13 2013-07-12 Combining biological micro-objects

Publications (2)

Publication Number Publication Date
JP2015530074A true JP2015530074A (ja) 2015-10-15
JP2015530074A5 JP2015530074A5 (enExample) 2016-09-01

Family

ID=49914304

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2015521839A Pending JP2015530074A (ja) 2012-07-13 2013-07-12 生物学的マイクロ物体の結合

Country Status (4)

Country Link
US (1) US20140017791A1 (enExample)
EP (1) EP2872615A4 (enExample)
JP (1) JP2015530074A (enExample)
WO (1) WO2014011985A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10973227B2 (en) 2015-04-22 2021-04-13 Berkeley Lights, Inc. Freezing and archiving cells on a microfluidic device
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. METHODS, SYSTEMS AND DEVICES FOR SELECTION AND PRODUCTION OF GENERICALLY WORKED CLONES
TWI795380B (zh) * 2016-12-01 2023-03-11 美商伯克利之光生命科技公司 針對微流體裝置中的微物件之自動偵測及重定位
JP7356351B2 (ja) 2016-12-12 2023-10-04 エクセラ・バイオサイエンシーズ・インコーポレイテッド マイクロキャピラリーアレイを使用したスクリーニングのための方法およびシステム
WO2018125832A1 (en) 2016-12-30 2018-07-05 xCella Biosciences, Inc. Multi-stage sample recovery system
CA3048645A1 (en) 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
JP7227162B2 (ja) 2017-06-06 2023-02-21 ザイマージェン インコーポレイテッド 真菌株を改良するためのｈｔｐゲノム操作プラットフォーム
SG11202011180UA (en) 2018-05-31 2020-12-30 Berkeley Lights Inc Automated detection and characterization of micro-objects in microfluidic devices
JP2021526799A (ja) 2018-06-06 2021-10-11 ザイマージェン インコーポレイテッド 発酵および産生中の真菌の形態を制御するためのシグナル伝達に関与する遺伝子の操作
ES3039910T3 (en) 2018-12-06 2025-10-27 Xcella Biosciences Inc Lateral loading of microcapillary arrays
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7901939B2 (en) * 2002-05-09 2011-03-08 University Of Chicago Method for performing crystallization and reactions in pressure-driven fluid plugs
US6958132B2 (en) * 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
WO2004103565A2 (de) * 2003-05-19 2004-12-02 Hans-Knöll-Institut für Naturstoff-Forschung e.V. Vorrichtung und verfahren zur strukturierung von flüssigkeiten und zum zudosieren von reaktionsflüssigkeiten zu in separationsmedium eingebetteten flüssigkeitskompartimenten
US20100273681A1 (en) * 2009-04-27 2010-10-28 Wisconsin Alumni Research Foundation Combinatorial chemistry reaction cell with optical tweezers

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007537729A (ja) * 2004-04-12 2007-12-27 ザ・レジェンツ・オブ・ザ・ユニバーシティ・オブ・カリフォルニア 微粒子及び細胞の操作用の光電子ピンセット

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KIRSCHBAUM, M., ET AL.: "Highly controlled electrofusion of individually selected cells in dielectrophoretic field cages", LAB ON A CHIP, vol. 12, no. 3, JPN6017017275, 2012, pages 443 - 450, ISSN: 0003557811 *
NING HU, N., ET AL.: "A high-throughput dielectrophoresis-based cell electrofusion microfluidic device", ELECTROPHORESIS, vol. 32, no. 18, JPN6017017273, 2011, pages 2488 - 2495, ISSN: 0003557810 *

Also Published As

Publication number Publication date
WO2014011985A1 (en) 2014-01-16
EP2872615A1 (en) 2015-05-20
EP2872615A4 (en) 2016-03-30
US20140017791A1 (en) 2014-01-16

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