JP2015521036A - イソプレン生産用のマメ科植物イソプレン合成酵素 - Google Patents

イソプレン生産用のマメ科植物イソプレン合成酵素 Download PDF

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JP2015521036A
JP2015521036A JP2015510461A JP2015510461A JP2015521036A JP 2015521036 A JP2015521036 A JP 2015521036A JP 2015510461 A JP2015510461 A JP 2015510461A JP 2015510461 A JP2015510461 A JP 2015510461A JP 2015521036 A JP2015521036 A JP 2015521036A
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polypeptide
isoprene
isoprene synthase
activity
host cell
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JP2015510461A
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Japanese (ja)
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JP2015521036A5 (enExample
Inventor
ビーティー、マリー・ケイ
ヘイズ、ケビン
フー、ジェンリン
メイヤー、デービッド・ジェイ
ナンナパネニ、キショー
ライフ、クリストファー・エル
ウェルス、デレク・エイチ
ザストロー−ヘイズ、ジーナ・エム
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Goodyear Tire and Rubber Co
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Goodyear Tire and Rubber Co
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Publication of JP2015521036A publication Critical patent/JP2015521036A/ja
Publication of JP2015521036A5 publication Critical patent/JP2015521036A5/ja
Withdrawn legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03027—Isoprene synthase (4.2.3.27)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
JP2015510461A 2012-05-02 2013-05-02 イソプレン生産用のマメ科植物イソプレン合成酵素 Withdrawn JP2015521036A (ja)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261641861P 2012-05-02 2012-05-02
US61/641,861 2012-05-02
US13/802,360 US8895277B2 (en) 2012-05-02 2013-03-13 Legume isoprene synthase for production of isoprene
US13/802,360 2013-03-13
PCT/US2013/039315 WO2013166320A1 (en) 2012-05-02 2013-05-02 Legume isoprene synthase for production of isoprene

Publications (2)

Publication Number Publication Date
JP2015521036A true JP2015521036A (ja) 2015-07-27
JP2015521036A5 JP2015521036A5 (enExample) 2016-06-30

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JP2015510461A Withdrawn JP2015521036A (ja) 2012-05-02 2013-05-02 イソプレン生産用のマメ科植物イソプレン合成酵素

Country Status (5)

Country Link
US (1) US8895277B2 (enExample)
EP (1) EP2844664A1 (enExample)
JP (1) JP2015521036A (enExample)
BR (1) BR112014027488A2 (enExample)
WO (1) WO2013166320A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017022856A1 (ja) * 2015-08-05 2017-02-09 味の素株式会社 イソプレンモノマーの製造方法
JP2021136905A (ja) * 2020-03-04 2021-09-16 国立大学法人千葉大学 変異型イソプレン合成酵素及びそのスクリーニング方法

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8647642B2 (en) 2008-09-18 2014-02-11 Aviex Technologies, Llc Live bacterial vaccines resistant to carbon dioxide (CO2), acidic PH and/or osmolarity for viral infection prophylaxis or treatment
US8962296B2 (en) * 2012-05-30 2015-02-24 Bridgestone Corporation Isoprene synthase and gene encoding the same, and method for producing isoprene monomer
JP6169073B2 (ja) * 2012-05-30 2017-07-26 株式会社ブリヂストン イソプレンシンターゼおよびそれをコードするポリヌクレオチド、ならびにイソプレンモノマーの製造方法
MY172689A (en) 2013-11-22 2019-12-10 Ajinomoto Kk Modified isoprene synthase
US10648004B2 (en) 2014-02-20 2020-05-12 Danisco Us Inc. Recombinant microorganisms for the enhanced production of mevalonate, isoprene, isoprenoid precursors, isoprenoids, and acetyl-CoA-derived products
KR102330593B1 (ko) 2014-07-28 2021-11-26 에스케이이노베이션 주식회사 신규 이소프렌 신타아제 및 이를 이용한 이소프렌의 제조방법
SG11201703132UA (en) 2014-10-22 2017-05-30 Temasek Life Sciences Lab Ltd Terpene synthases from ylang ylang (cananga odorata var. fruticosa)
WO2016129637A1 (ja) * 2015-02-13 2016-08-18 積水化学工業株式会社 核酸、融合タンパク質、組換え細胞、並びに、イソプレン又は環式テルペンの生産方法
US10676723B2 (en) 2015-05-11 2020-06-09 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
KR102546640B1 (ko) * 2016-01-26 2023-06-22 에스케이이노베이션 주식회사 재조합 호염성 메탄균주를 이용한 이소프렌의 제조방법
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
WO2019006257A1 (en) * 2017-06-30 2019-01-03 Invista North America .S.A.R.L. METHODS, SYNTHETIC HOSTS AND REAGENTS FOR HYDROCARBON BIOSYNTHESIS
US11634733B2 (en) 2017-06-30 2023-04-25 Inv Nylon Chemicals Americas, Llc Methods, materials, synthetic hosts and reagents for the biosynthesis of hydrocarbons and derivatives thereof
US11505809B2 (en) 2017-09-28 2022-11-22 Inv Nylon Chemicals Americas Llc Organisms and biosynthetic processes for hydrocarbon synthesis

Family Cites Families (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8407828D0 (en) 1984-03-27 1984-05-02 Ontario Research Foundation In situ preparation
US4570029A (en) 1985-03-04 1986-02-11 Uop Inc. Process for separating isoprene
US5849970A (en) 1995-06-23 1998-12-15 The Regents Of The University Of Colorado Materials and methods for the bacterial production of isoprene
DE19629568C1 (de) 1996-07-15 1998-01-08 Fraunhofer Ges Forschung Verfahren zur Herstellung von Isopren
JP3666484B2 (ja) * 2001-06-21 2005-06-29 東洋ゴム工業株式会社 液封入式防振装置
US20050287655A1 (en) 2002-05-10 2005-12-29 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
AU2003287028B2 (en) 2002-10-04 2008-09-04 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
RU2004124226A (ru) 2004-08-10 2006-01-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) Использование фосфокетолазы для продукции полезных метаболитов
CA2651747C (en) 2006-05-26 2017-10-24 Amyris Biotechnologies, Inc. Production of isoprenoids
US7947478B2 (en) * 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
JP4986547B2 (ja) 2006-09-04 2012-07-25 株式会社Ａｄｅｋａ 新規なアセトアセチルＣｏＡ合成酵素、それをコードするＤＮＡ配列、当該酵素の製造方法および当該酵素を利用したメバロン酸の製造方法
BRPI0823506A8 (pt) 2007-12-13 2018-05-15 Danisco Us Inc Genencor Div composições e métodos para a produção de isoprene
WO2009100231A2 (en) 2008-02-06 2009-08-13 The Regents Of The University Of California Short chain volatile isoprene hydrocarbon production using the mevalonic acid pathway in genetically engineered yeast and fungi
JP2011518564A (ja) 2008-04-23 2011-06-30 ダニスコ・ユーエス・インク 改良された微生物によるイソプレン産出用のイソプレンシンターゼ変異体
US7632995B2 (en) 2008-05-09 2009-12-15 Monsanto Technology Llc Plants and seeds of hybrid corn variety CH852179
EP2310490B1 (en) 2008-07-02 2019-01-02 Danisco US Inc. Methods for producing isoprene
HU3516U (en) 2008-07-31 2008-11-28 Zoltan Kuthi Anti-theft protection of draining for fuel tanks
CA2737082A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Increased isoprene production using mevalonate kinase and isoprene synthase
CA2737223A1 (en) 2008-09-15 2010-03-18 Danisco Us Inc. Reduction of carbon dioxide emission during isoprene production by fermentation
MY156562A (en) 2008-09-15 2016-02-26 Danisco Us Inc Increased isoprene production using the archaeal lower mevalonate pathway
BRPI0918936A2 (pt) 2008-09-15 2019-09-24 Danisco Us Inc conversão de derivados de prenila em isopreno
WO2010031079A1 (en) * 2008-09-15 2010-03-18 Danisco Us Inc. Systems using cell culture for production of isoprene
CN102333866B (zh) 2008-12-30 2015-04-29 丹尼斯科美国公司 生产异戊二烯和共同产物的方法
US20100297749A1 (en) 2009-04-21 2010-11-25 Sapphire Energy, Inc. Methods and systems for biofuel production
EP2421966A2 (en) * 2009-04-23 2012-02-29 Danisco US Inc. Three-dimensional structure of isoprene synthase and its use thereof for generating variants
JP4760951B2 (ja) 2009-05-08 2011-08-31 トヨタ自動車株式会社 ブタノール生産能を有する組換え微生物及びブタノールの製造方法
TWI434921B (zh) 2009-06-17 2014-04-21 Danisco Us Inc 從生物異戊二烯組合物製造燃料成分之方法及系統
TW201412988A (zh) 2009-06-17 2014-04-01 Danisco Us Inc 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
CA2773675A1 (en) 2009-09-15 2011-03-24 Sapphire Energy, Inc. A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN102884028B (zh) 2009-12-18 2015-08-19 丹尼斯科美国公司 从可再生资源中纯化异戊二烯
AU2010336342B2 (en) 2009-12-23 2015-02-26 Danisco Us Inc. Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (PGL)
US8871488B2 (en) 2010-06-18 2014-10-28 Butamax Advanced Biofuels Llc Recombinant host cells comprising phosphoketolases
JP2014502148A (ja) * 2010-10-27 2014-01-30 ダニスコ・ユーエス・インク イソプレン生産の向上を目的としたイソプレン合成酵素変異体

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017022856A1 (ja) * 2015-08-05 2017-02-09 味の素株式会社 イソプレンモノマーの製造方法
JP2021136905A (ja) * 2020-03-04 2021-09-16 国立大学法人千葉大学 変異型イソプレン合成酵素及びそのスクリーニング方法
JP7565569B2 (ja) 2020-03-04 2024-10-11 国立大学法人千葉大学 変異型イソプレン合成酵素及びそのスクリーニング方法

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Publication number Publication date
EP2844664A1 (en) 2015-03-11
BR112014027488A2 (pt) 2017-08-08
US20130330709A1 (en) 2013-12-12
WO2013166320A1 (en) 2013-11-07
US8895277B2 (en) 2014-11-25

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