JP2015513666A5 - - Google Patents
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- JP2015513666A5 JP2015513666A5 JP2014557833A JP2014557833A JP2015513666A5 JP 2015513666 A5 JP2015513666 A5 JP 2015513666A5 JP 2014557833 A JP2014557833 A JP 2014557833A JP 2014557833 A JP2014557833 A JP 2014557833A JP 2015513666 A5 JP2015513666 A5 JP 2015513666A5
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| WO2015065995A1 (en) * | 2013-10-28 | 2015-05-07 | Trustees Of Boston University | Nanoparticles for self referencing calibration |
| RU2732791C2 (ru) * | 2014-12-31 | 2020-09-22 | Конинклейке Филипс Н.В. | Иммобилизация модифицированного олигонуклеотида на полимерном субстрате посредством физической адсорбции |
| JP2018503830A (ja) * | 2015-02-02 | 2018-02-08 | ツー ポア ガイズ インコーポレイテッド | バイオマーカー検出のための不安定なリンカー |
| US20190127540A1 (en) * | 2017-10-31 | 2019-05-02 | Tokyo Ohka Kogyo Co., Ltd. | Surface treatment method, surface treatment liquid, and surface-treated article |
| WO2019241794A1 (en) * | 2018-06-16 | 2019-12-19 | Hacker Kevin Jay | Microorganism identification and characterization |
| CN111620985B (zh) * | 2019-02-28 | 2023-08-08 | 康码（上海）生物科技有限公司 | 共价固定dna的水凝胶及其应用 |
| US11725073B2 (en) | 2020-12-29 | 2023-08-15 | Hongene Biotech Corporation | Compositions and methods for liquid phase oligonucleotide synthesis |
| KR102814714B1 (ko) * | 2020-12-31 | 2025-05-29 | 주식회사 아이센스 | 펜타플루오로페닐 에스터를 포함하는 고분자 및 이를 포함하는 전기화학적 바이오센서 |
| MX2023011749A (es) * | 2021-04-30 | 2024-01-12 | Illumina Cambridge Ltd | Celda de flujo y metodos. |
| US11851454B2 (en) | 2021-12-30 | 2023-12-26 | Hongene Biotech Corporation | Compositions and methods for liquid phase oligonucleotide synthesis |
| CN119998357A (zh) | 2022-06-21 | 2025-05-13 | 兆维生物科技公司 | 用于液相寡核苷酸合成的组合物和方法 |
| WO2024137756A1 (en) | 2022-12-23 | 2024-06-27 | Hongene Biotech Corporation | Compounds and methods for liquid phase oligonucleotide synthesis |
| CN116425192B (zh) * | 2023-02-06 | 2023-11-14 | 北京伽瓦新材料科技有限公司 | Ps-pvd专用粉体材料、制备方法及应用 |
Family Cites Families (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3996345A (en) | 1974-08-12 | 1976-12-07 | Syva Company | Fluorescence quenching with immunological pairs in immunoassays |
| US4351760A (en) | 1979-09-07 | 1982-09-28 | Syva Company | Novel alkyl substituted fluorescent compounds and polyamino acid conjugates |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4739044A (en) | 1985-06-13 | 1988-04-19 | Amgen | Method for derivitization of polynucleotides |
| US4757141A (en) | 1985-08-26 | 1988-07-12 | Applied Biosystems, Incorporated | Amino-derivatized phosphite and phosphate linking agents, phosphoramidite precursors, and useful conjugates thereof |
| US5231191A (en) | 1987-12-24 | 1993-07-27 | Applied Biosystems, Inc. | Rhodamine phosphoramidite compounds |
| US4997928A (en) | 1988-09-15 | 1991-03-05 | E. I. Du Pont De Nemours And Company | Fluorescent reagents for the preparation of 5'-tagged oligonucleotides |
| WO1998022542A2 (en) * | 1996-11-08 | 1998-05-28 | Ikonos Corporation | Chemical functionalization of surfaces |
| US6060242A (en) | 1997-02-27 | 2000-05-09 | Lorne Park Research, Inc. | PNA diagnostic methods |
| US5932711A (en) | 1997-03-05 | 1999-08-03 | Mosaic Technologies, Inc. | Nucleic acid-containing polymerizable complex |
| US6994972B2 (en) | 1999-09-02 | 2006-02-07 | Corning Incorporated | Porous substrates for DNA arrays |
| US6790613B1 (en) | 1999-11-12 | 2004-09-14 | Amersham Biosciences Ab | Method of preparing an oligonucleotide array |
| US7217512B2 (en) | 2002-05-09 | 2007-05-15 | Corning Incorporated | Reagent and method for attaching target molecules to a surface |
| US20040076961A1 (en) | 2002-10-21 | 2004-04-22 | Lewis Mark A. | Biomolecule retaining material and methods for attaching biomolecules to a surface |
| JP2004300313A (ja) * | 2003-03-31 | 2004-10-28 | Nof Corp | 撥水撥油性ブロック共重合体およびその製造方法 |
| US7309593B2 (en) * | 2003-10-01 | 2007-12-18 | Surmodics, Inc. | Attachment of molecules to surfaces |
| WO2007041394A2 (en) * | 2005-09-30 | 2007-04-12 | Pacific Biosciences Of California, Inc. | Reactive surfaces, substrates and methods for producing and using same |
| US7781203B2 (en) | 2005-12-29 | 2010-08-24 | Corning Incorporated | Supports for assaying analytes and methods of making and using thereof |
| US8597959B2 (en) * | 2007-04-19 | 2013-12-03 | 3M Innovative Properties Company | Methods of use of solid support material for binding biomolecules |
| JP5601758B2 (ja) * | 2008-03-31 | 2014-10-08 | ダイキン工業株式会社 | 表面処理剤および撥水撥油処理方法 |
| US8470906B2 (en) * | 2008-09-30 | 2013-06-25 | Johnson & Johnson Vision Care, Inc. | Ionic silicone hydrogels having improved hydrolytic stability |
| ES2401965T3 (es) * | 2009-02-19 | 2013-04-25 | Novo Nordisk A/S | Modificación de Factor VIII |
| US8043369B2 (en) * | 2009-06-16 | 2011-10-25 | Bausch & Lomb Incorporated | Biomedical devices |
-
2013
- 2013-02-15 WO PCT/US2013/026455 patent/WO2013123409A1/en not_active Ceased
- 2013-02-15 EP EP13707970.3A patent/EP2814857A1/en not_active Withdrawn
- 2013-02-15 SG SG11201404892UA patent/SG11201404892UA/en unknown
- 2013-02-15 JP JP2014557833A patent/JP2015513666A/ja active Pending
- 2013-02-15 KR KR20147026011A patent/KR20140137366A/ko not_active Withdrawn
- 2013-02-15 HK HK15105826.2A patent/HK1205163A1/xx unknown
- 2013-02-15 US US13/769,123 patent/US20130231260A1/en not_active Abandoned
- 2013-02-15 CN CN201380020388.5A patent/CN104321355A/zh active Pending
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