JP2015509724A5 - - Google Patents
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- Publication number
- JP2015509724A5 JP2015509724A5 JP2014559299A JP2014559299A JP2015509724A5 JP 2015509724 A5 JP2015509724 A5 JP 2015509724A5 JP 2014559299 A JP2014559299 A JP 2014559299A JP 2014559299 A JP2014559299 A JP 2014559299A JP 2015509724 A5 JP2015509724 A5 JP 2015509724A5
- Authority
- JP
- Japan
- Prior art keywords
- vdj
- product
- recombinant
- cell population
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 32
- 210000004027 cell Anatomy 0.000 claims 14
- 238000005215 recombination Methods 0.000 claims 9
- 230000006798 recombination Effects 0.000 claims 9
- 239000012634 fragment Substances 0.000 claims 7
- 108091008146 restriction endonucleases Proteins 0.000 claims 7
- 108020004414 DNA Proteins 0.000 claims 6
- 108091034117 Oligonucleotide Proteins 0.000 claims 6
- 101150008942 J gene Proteins 0.000 claims 4
- 108060003951 Immunoglobulin Proteins 0.000 claims 3
- 210000005260 human cell Anatomy 0.000 claims 3
- 102000018358 immunoglobulin Human genes 0.000 claims 3
- 238000012163 sequencing technique Methods 0.000 claims 3
- 238000011144 upstream manufacturing Methods 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- 208000029462 Immunodeficiency disease Diseases 0.000 claims 2
- 101150117115 V gene Proteins 0.000 claims 2
- 238000000527 sonication Methods 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 206010025323 Lymphomas Diseases 0.000 claims 1
- 108020004511 Recombinant DNA Proteins 0.000 claims 1
- 108010090804 Streptavidin Proteins 0.000 claims 1
- 108091008874 T cell receptors Proteins 0.000 claims 1
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims 1
- 210000001744 T-lymphocyte Anatomy 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 239000000427 antigen Substances 0.000 claims 1
- 108091007433 antigens Proteins 0.000 claims 1
- 102000036639 antigens Human genes 0.000 claims 1
- 210000003719 b-lymphocyte Anatomy 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 238000009396 hybridization Methods 0.000 claims 1
- 230000016784 immunoglobulin production Effects 0.000 claims 1
- 238000000338 in vitro Methods 0.000 claims 1
- 208000032839 leukemia Diseases 0.000 claims 1
- 210000000265 leukocyte Anatomy 0.000 claims 1
- 210000004698 lymphocyte Anatomy 0.000 claims 1
- 238000012544 monitoring process Methods 0.000 claims 1
- 238000010839 reverse transcription Methods 0.000 claims 1
- 238000002702 ribosome display Methods 0.000 claims 1
- 238000010008 shearing Methods 0.000 claims 1
- 238000011830 transgenic mouse model Methods 0.000 claims 1
- 230000000007 visual effect Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1203720.6A GB201203720D0 (en) | 2012-03-02 | 2012-03-02 | Method of identifying VDJ recombination products |
| GB1203720.6 | 2012-03-02 | ||
| PCT/GB2013/050516 WO2013128204A1 (en) | 2012-03-02 | 2013-03-01 | Method of identifying vdj recombination products |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2015509724A JP2015509724A (ja) | 2015-04-02 |
| JP2015509724A5 true JP2015509724A5 (enExample) | 2016-04-14 |
| JP6513949B2 JP6513949B2 (ja) | 2019-05-22 |
Family
ID=46003032
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2014559299A Expired - Fee Related JP6513949B2 (ja) | 2012-03-02 | 2013-03-01 | Ｖｄｊ組み換え産物を特定する方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US9797014B2 (enExample) |
| EP (1) | EP2820153B1 (enExample) |
| JP (1) | JP6513949B2 (enExample) |
| GB (1) | GB201203720D0 (enExample) |
| WO (1) | WO2013128204A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3063295B1 (en) | 2013-10-29 | 2017-12-13 | Region Syddanmark | Method for analyzing body fluid samples |
| US12043856B2 (en) | 2016-05-24 | 2024-07-23 | The Translational Genomics Research Institute | Molecular tagging methods and sequencing libraries |
| US11674137B2 (en) * | 2016-05-27 | 2023-06-13 | Haplox Biotechnology (Shenzhen) Co., Ltd. | Adaptor for sequencing DNA at ultratrace level and use thereof |
| ES2977787T3 (es) * | 2017-01-31 | 2024-08-30 | Ludwig Inst For Cancer Res Ltd | Métodos mejorados de secuenciación de receptores de células inmunitarias |
| US20200131564A1 (en) * | 2017-07-07 | 2020-04-30 | Board Of Regents, The University Of Texas System | High-coverage and ultra-accurate immune repertoire sequencing using molecular identifiers |
| EP3695010A4 (en) * | 2017-10-10 | 2021-11-17 | Memorial Sloan-Kettering Cancer Center | PRIMER EXTRACTION AND CLONALITY DETECTION SYSTEM AND METHODS |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2671356B1 (fr) | 1991-01-09 | 1993-04-30 | Inst Nat Sante Rech Med | Procede de description des repertoires d'anticorps (ab) et des recepteurs des cellules t (tcr) du systeme immunitaire d'un individu. |
| US6221600B1 (en) * | 1999-10-08 | 2001-04-24 | Board Of Regents, The University Of Texas System | Combinatorial oligonucleotide PCR: a method for rapid, global expression analysis |
| EP2460889B1 (en) * | 2002-10-11 | 2013-11-20 | Erasmus Universiteit Rotterdam | Nucleic acid amplification primers for PCR-based clonality studies of BCL2-IGH rearrangements |
| US8642513B2 (en) * | 2005-09-15 | 2014-02-04 | Crucell Holland B.V. | Method for preparing immunoglobulin libraries |
| WO2008026927A2 (en) * | 2006-08-30 | 2008-03-06 | Academisch Medisch Centrum | Process for displaying t- and b-cell receptor repertoires |
| CA2796822C (en) * | 2010-05-07 | 2021-10-05 | The Board Of Trustees Of The Leland Standford Junior University | Measurement and comparison of immune diversity by high-throughput sequencing |
| EP2625295B1 (en) * | 2010-10-08 | 2019-03-13 | President and Fellows of Harvard College | High-throughput immune sequencing |
-
2012
- 2012-03-02 GB GBGB1203720.6A patent/GB201203720D0/en not_active Ceased
-
2013
- 2013-03-01 US US14/382,142 patent/US9797014B2/en not_active Expired - Fee Related
- 2013-03-01 WO PCT/GB2013/050516 patent/WO2013128204A1/en not_active Ceased
- 2013-03-01 EP EP13709986.7A patent/EP2820153B1/en not_active Not-in-force
- 2013-03-01 JP JP2014559299A patent/JP6513949B2/ja not_active Expired - Fee Related
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