JP2015505241A - 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット - Google Patents

生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット Download PDF

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JP2015505241A
JP2015505241A JP2014546191A JP2014546191A JP2015505241A JP 2015505241 A JP2015505241 A JP 2015505241A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2015505241 A JP2015505241 A JP 2015505241A
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nucleic acid
target nucleic
probe
sample
hybridization
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JP2015505241A5 (enExample
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オーリッヒ−コスタ,ジョーン
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セレー,インコーポレイテッド
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014546191A 2011-12-12 2012-12-12 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット Pending JP2015505241A (ja)

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Application Number Priority Date Filing Date Title
US201161569656P 2011-12-12 2011-12-12
US61/569,656 2011-12-12
PCT/US2012/069178 WO2013090386A2 (en) 2011-12-12 2012-12-12 Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample

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JP2015505241A true JP2015505241A (ja) 2015-02-19
JP2015505241A5 JP2015505241A5 (enExample) 2016-01-28

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JP2014546191A Pending JP2015505241A (ja) 2011-12-12 2012-12-12 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット

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US (2) US9145584B2 (enExample)
EP (1) EP2802671B1 (enExample)
JP (1) JP2015505241A (enExample)
CN (2) CN104145027A (enExample)
BR (1) BR112014014307A2 (enExample)
DK (1) DK2802671T3 (enExample)
WO (1) WO2013090386A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9145584B2 (en) 2011-12-12 2015-09-29 Cellay, Inc. Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
US20140161972A1 (en) * 2012-12-09 2014-06-12 National Sun Yat-Sen University Method for forming conductive film at room temperature
AU2014346735A1 (en) 2013-11-07 2016-06-09 Cellay, Inc. Methods and kits for detecting a prostate carcinoma and predicting disease outcomes for prostate cancers
US11021739B2 (en) * 2015-02-04 2021-06-01 Alma Mater Studiorum Uneverseta Di Bologna Additive for accelerating hybridization
CN108779490A (zh) * 2016-01-08 2018-11-09 雅培分子公司 包含硫氰酸胍的杂交缓冲液
US10577645B2 (en) * 2016-03-18 2020-03-03 Norgen Biotek Corp. Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA
KR102026683B1 (ko) * 2016-08-24 2019-09-30 울산대학교 산학협력단 Dtbp를 이용한 미생물 농축 또는 핵산 추출 방법
WO2021061841A1 (en) * 2019-09-23 2021-04-01 Element Biosciences, Inc. Methods for cellularly addressable nucleic acid sequencing
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
CA3264896A1 (en) 2022-08-15 2024-02-22 Element Biosciences, Inc. SPATIAL RESOLUTION SURFACE CAPTURE OF NUCLEIC ACIDS

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523100A (ja) * 2003-04-03 2006-10-12 モナリザ メディカル, リミテッド 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断
JP2008535508A (ja) * 2005-04-14 2008-09-04 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753433A (en) * 1909-12-05 1998-05-19 Boehringer Mannheim Gmbh Method for the sensitive detection of nucleic acids
US5753437A (en) 1987-10-13 1998-05-19 The United States Of America As Represented By The Department Of Health & Human Services Method of diagnosing cancer susceptibility or metastatic potential
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
NL9001639A (nl) 1990-07-19 1992-02-17 Amc Amsterdam Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen.
US5714327A (en) 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
US5719024A (en) 1993-08-18 1998-02-17 Applied Spectral Imaging Ltd. Method for chromosome classification by decorrelation statistical analysis and hardware therefore
NZ307633A (en) 1995-05-09 2000-01-28 Kreatech Biotechnology Bv Methods for the production of platinum-based linkers between labels and bio-organic molecules, for labelling bio-organic molecules, for detecting
WO1997004133A1 (en) 1995-07-21 1997-02-06 Regents Of The University Of Minnesota Analysis of alpha integrins for the diagnosis of diabetic nephropathy
AU741839B2 (en) 1996-10-04 2001-12-13 Novo Nordisk A/S N-substituted azaheterocyclic compounds
US20020197656A1 (en) 1999-12-17 2002-12-26 Ronghao Li Cell arrays and the uses thereof
US20040115692A1 (en) 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
US6617148B2 (en) 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
US6825330B2 (en) 2001-03-02 2004-11-30 Stratagene California Compositions and methods using platinum compounds for nucleic acid labeling
EP1590661A4 (en) 2002-11-05 2006-07-26 Roskamp Res Llc GENE OF PROTEIN-4 (OATPRP4) BITOURETTE SYNDROME AND RELATED DISEASES RELATED TO THE POLYPEPTIDE FOR DENTRANSPORT OF ORGANIC ANIONS
US6995020B2 (en) 2003-07-21 2006-02-07 Aureon Laboratories, Inc. Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization
WO2005121373A2 (en) 2004-06-03 2005-12-22 Advandx, Inc. Hybridization of pna probes in alcohol solutions
US20090215643A1 (en) * 2007-07-26 2009-08-27 Cellay, Llc Highly Visible Chromosome-Specific Probes and Related Methods
US20090036664A1 (en) 2007-07-31 2009-02-05 Brian Jon Peter Complex oligonucleotide primer mix
WO2009041917A1 (en) * 2007-09-28 2009-04-02 Agency For Science, Technology And Research Method of electrically detecting a nucleic acid molecule
CN101363048B (zh) * 2008-08-29 2011-11-23 芮屈生物技术(上海)有限公司 一种nkx2-8基因的原位杂交检测试剂盒及其检测方法和应用
DK2576830T3 (en) 2010-06-03 2014-12-08 Cellay Inc Methods for in situ detection of nucleotide sequences
US9145584B2 (en) 2011-12-12 2015-09-29 Cellay, Inc. Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523100A (ja) * 2003-04-03 2006-10-12 モナリザ メディカル, リミテッド 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断
JP2008535508A (ja) * 2005-04-14 2008-09-04 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. EXP. CLIN. CANCER RES., vol. 30:38, JPN6016035352, 2011, ISSN: 0003399866 *

Also Published As

Publication number Publication date
WO2013090386A3 (en) 2013-08-22
US20130149705A1 (en) 2013-06-13
US10400275B2 (en) 2019-09-03
EP2802671B1 (en) 2018-07-18
DK2802671T3 (en) 2018-11-05
BR112014014307A2 (pt) 2019-09-24
WO2013090386A2 (en) 2013-06-20
CN105385757B (zh) 2020-04-03
US9145584B2 (en) 2015-09-29
EP2802671A2 (en) 2014-11-19
CN105385757A (zh) 2016-03-09
CN104145027A (zh) 2014-11-12
US20150368701A1 (en) 2015-12-24

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