JP2015505241A - 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット - Google Patents

生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット Download PDF

Info

Publication number
JP2015505241A
JP2015505241A JP2014546191A JP2014546191A JP2015505241A JP 2015505241 A JP2015505241 A JP 2015505241A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2014546191 A JP2014546191 A JP 2014546191A JP 2015505241 A JP2015505241 A JP 2015505241A
Authority
JP
Japan
Prior art keywords
nucleic acid
target nucleic
probe
sample
hybridization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2014546191A
Other languages
English (en)
Japanese (ja)
Other versions
JP2015505241A5 (enExample
Inventor
オーリッヒ−コスタ,ジョーン
Original Assignee
セレー,インコーポレイテッド
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by セレー,インコーポレイテッド filed Critical セレー,インコーポレイテッド
Publication of JP2015505241A publication Critical patent/JP2015505241A/ja
Publication of JP2015505241A5 publication Critical patent/JP2015505241A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2014546191A 2011-12-12 2012-12-12 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット Pending JP2015505241A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161569656P 2011-12-12 2011-12-12
US61/569,656 2011-12-12
PCT/US2012/069178 WO2013090386A2 (en) 2011-12-12 2012-12-12 Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample

Publications (2)

Publication Number Publication Date
JP2015505241A true JP2015505241A (ja) 2015-02-19
JP2015505241A5 JP2015505241A5 (enExample) 2016-01-28

Family

ID=47436256

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014546191A Pending JP2015505241A (ja) 2011-12-12 2012-12-12 生物学的試料中の標的核酸の室温インサイチュ検出のための方法およびキット

Country Status (7)

Country Link
US (2) US9145584B2 (enExample)
EP (1) EP2802671B1 (enExample)
JP (1) JP2015505241A (enExample)
CN (2) CN105385757B (enExample)
BR (1) BR112014014307A2 (enExample)
DK (1) DK2802671T3 (enExample)
WO (1) WO2013090386A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9145584B2 (en) 2011-12-12 2015-09-29 Cellay, Inc. Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
US20140161972A1 (en) * 2012-12-09 2014-06-12 National Sun Yat-Sen University Method for forming conductive film at room temperature
AU2014346735A1 (en) 2013-11-07 2016-06-09 Cellay, Inc. Methods and kits for detecting a prostate carcinoma and predicting disease outcomes for prostate cancers
CN107406877B (zh) * 2015-02-04 2021-06-18 博洛尼亚大学 用于加快杂交反应的添加剂
US10457981B2 (en) * 2016-01-08 2019-10-29 Abbott Molecular Inc. Hybridization buffers
US10577645B2 (en) * 2016-03-18 2020-03-03 Norgen Biotek Corp. Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA
EP3505628B1 (en) * 2016-08-24 2023-05-03 Infusion Tech Method for concentrating microorganism or extracting nucleic acid using dtbp
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
AU2020354551A1 (en) * 2019-09-23 2022-04-14 Element Biosciences, Inc. Methods for cellularly addressable nucleic acid sequencing
AU2023325064A1 (en) 2022-08-15 2025-03-06 Element Biosciences, Inc. Spatially resolved surface capture of nucleic acids

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523100A (ja) * 2003-04-03 2006-10-12 モナリザ メディカル, リミテッド 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断
JP2008535508A (ja) * 2005-04-14 2008-09-04 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5753433A (en) * 1909-12-05 1998-05-19 Boehringer Mannheim Gmbh Method for the sensitive detection of nucleic acids
US5753437A (en) 1987-10-13 1998-05-19 The United States Of America As Represented By The Department Of Health & Human Services Method of diagnosing cancer susceptibility or metastatic potential
US5082830A (en) 1988-02-26 1992-01-21 Enzo Biochem, Inc. End labeled nucleotide probe
NL9001639A (nl) 1990-07-19 1992-02-17 Amc Amsterdam Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen.
US5714327A (en) 1990-07-19 1998-02-03 Kreatech Diagnostics Platinum-containing compounds, methods for their preparation and applications thereof
US5719024A (en) 1993-08-18 1998-02-17 Applied Spectral Imaging Ltd. Method for chromosome classification by decorrelation statistical analysis and hardware therefore
DE69629403T2 (de) 1995-05-09 2004-06-24 Kreatech Biotechnology B.V. Verfahren zur herstellung von linkern zwischen markierungsgruppen und bio-organischen molekülen auf der basis von platin für die markierung von bio-organischen molekülen zur entdeckung interessanter biologischer substanzen
WO1997004133A1 (en) 1995-07-21 1997-02-06 Regents Of The University Of Minnesota Analysis of alpha integrins for the diagnosis of diabetic nephropathy
PL332593A1 (en) 1996-10-04 1999-09-27 Novo Nordisk As N-substituted azaheterocyclic compounds
US20020197656A1 (en) 1999-12-17 2002-12-26 Ronghao Li Cell arrays and the uses thereof
US20040115692A1 (en) 2000-04-03 2004-06-17 Cytyc Corporation Methods, compositions and apparatuses for detecting a target in a preservative solution
US6617148B2 (en) 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
WO2002069898A2 (en) 2001-03-02 2002-09-12 Stratagene Compositions and methods using platinum compounds for nucleic acid labeling
WO2004042035A2 (en) 2002-11-05 2004-05-21 Roskamp Research Llc Organic anion transport polypeptide related protein-4 (oatprp4) gene in tourette syndrome and related disorders
US6995020B2 (en) * 2003-07-21 2006-02-07 Aureon Laboratories, Inc. Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization
WO2005121373A2 (en) 2004-06-03 2005-12-22 Advandx, Inc. Hybridization of pna probes in alcohol solutions
US20090215643A1 (en) * 2007-07-26 2009-08-27 Cellay, Llc Highly Visible Chromosome-Specific Probes and Related Methods
US20090036664A1 (en) 2007-07-31 2009-02-05 Brian Jon Peter Complex oligonucleotide primer mix
US20100285601A1 (en) * 2007-09-28 2010-11-11 Agency For Science, Technology And Research Method of electrically detecting a nucleic acid molecule
CN101363048B (zh) * 2008-08-29 2011-11-23 芮屈生物技术(上海)有限公司 一种nkx2-8基因的原位杂交检测试剂盒及其检测方法和应用
EP2576830B1 (en) 2010-06-03 2014-08-27 Cellay, Inc. Methods for in situ detection of nucleotide sequences
US9145584B2 (en) 2011-12-12 2015-09-29 Cellay, Inc. Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2006523100A (ja) * 2003-04-03 2006-10-12 モナリザ メディカル, リミテッド 経子宮頸細胞を使用する非侵襲的出生前遺伝子診断
JP2008535508A (ja) * 2005-04-14 2008-09-04 メルク パテント ゲゼルシャフト ミット ベシュレンクテル ハフトング 腫瘍組織における増大したコピー数のegfr遺伝子に基づく抗egfr抗体療法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
J. EXP. CLIN. CANCER RES., vol. 30:38, JPN6016035352, 2011, ISSN: 0003399866 *

Also Published As

Publication number Publication date
WO2013090386A2 (en) 2013-06-20
WO2013090386A3 (en) 2013-08-22
US20130149705A1 (en) 2013-06-13
BR112014014307A2 (pt) 2019-09-24
US9145584B2 (en) 2015-09-29
CN105385757A (zh) 2016-03-09
DK2802671T3 (en) 2018-11-05
CN104145027A (zh) 2014-11-12
EP2802671A2 (en) 2014-11-19
EP2802671B1 (en) 2018-07-18
US20150368701A1 (en) 2015-12-24
US10400275B2 (en) 2019-09-03
CN105385757B (zh) 2020-04-03

Similar Documents

Publication Publication Date Title
US9145584B2 (en) Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample
DK2576830T3 (en) Methods for in situ detection of nucleotide sequences
RU2505609C2 (ru) Композиции и способы гибридизации
EP2914737B1 (en) Methods and kits for performing in situ hybridization
CA2116214C (en) In situ hybridization method
US20240084395A1 (en) Single-stranded oligonucleotide probes for chromosome or gene copy enumeration
EP1356116A2 (en) Hybridization buffers using low molecular weight dextran sulfate and methods for their use
AU2002240180A1 (en) Hybridization buffers using low molecular weight dextran sulfate and methods for their use
US20160046984A1 (en) Robust Detection of Nucleic Acids in Situ
CN108779490A (zh) 包含硫氰酸胍的杂交缓冲液
Kearney et al. Fluorescence in situ hybridization for cancer-related studies
Knoll et al. In situ hybridization and detection using nonisotopic probes
US20230093993A1 (en) Methods And Kits For Detecting A Prostate Carcinoma And Predicting Disease Outcomes For Prostate Cancers
GOSDEN et al. Primed in situ DNA synthesis (PRINS)
Sterchi et al. Molecular pathology 21

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20151204

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20151204

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20160913

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20161212

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20170522