DK2802671T3 - METHODS AND KITS FOR ROOM TEMPERATURE-IN SITU DETECTION OF A TARGET-NUCLEIC ACID IN A BIOLOGICAL SAMPLE - Google Patents
METHODS AND KITS FOR ROOM TEMPERATURE-IN SITU DETECTION OF A TARGET-NUCLEIC ACID IN A BIOLOGICAL SAMPLE Download PDFInfo
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- DK2802671T3 DK2802671T3 DK12806823.6T DK12806823T DK2802671T3 DK 2802671 T3 DK2802671 T3 DK 2802671T3 DK 12806823 T DK12806823 T DK 12806823T DK 2802671 T3 DK2802671 T3 DK 2802671T3
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- Prior art keywords
- nucleic acid
- target nucleic
- sample
- probe
- hybridization
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- WGTODYJZXSJIAG-UHFFFAOYSA-N tetramethylrhodamine chloride Chemical compound [Cl-].C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C(O)=O WGTODYJZXSJIAG-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6841—In situ hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161569656P | 2011-12-12 | 2011-12-12 | |
| PCT/US2012/069178 WO2013090386A2 (en) | 2011-12-12 | 2012-12-12 | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2802671T3 true DK2802671T3 (en) | 2018-11-05 |
Family
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|---|---|
| US (2) | US9145584B2 (enExample) |
| EP (1) | EP2802671B1 (enExample) |
| JP (1) | JP2015505241A (enExample) |
| CN (2) | CN105385757B (enExample) |
| BR (1) | BR112014014307A2 (enExample) |
| DK (1) | DK2802671T3 (enExample) |
| WO (1) | WO2013090386A2 (enExample) |
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| EP2802671B1 (en) | 2011-12-12 | 2018-07-18 | Cellay, Inc. | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample |
| US20140161972A1 (en) * | 2012-12-09 | 2014-06-12 | National Sun Yat-Sen University | Method for forming conductive film at room temperature |
| EP3066214B1 (en) | 2013-11-07 | 2019-10-02 | Cellay, Inc. | Methods and kits for detecting a prostate carcinoma and predicting disease outcomes for prostate cancers |
| ES2796701T3 (es) * | 2015-02-04 | 2020-11-30 | Univ Bologna Alma Mater Studiorum | Aditivo para acelerar la hibridación |
| JP2019502386A (ja) * | 2016-01-08 | 2019-01-31 | アボツト・モレキユラー・インコーポレイテツド | グアニジウムチオシアネートを含むハイブリダイゼーション緩衝液 |
| US10577645B2 (en) * | 2016-03-18 | 2020-03-03 | Norgen Biotek Corp. | Methods and kits for improving global gene expression analysis of human blood, plasma and/or serum derived RNA |
| EP3505628B1 (en) | 2016-08-24 | 2023-05-03 | Infusion Tech | Method for concentrating microorganism or extracting nucleic acid using dtbp |
| US11287422B2 (en) | 2019-09-23 | 2022-03-29 | Element Biosciences, Inc. | Multivalent binding composition for nucleic acid analysis |
| GB2635867B (en) * | 2019-09-23 | 2025-12-03 | Element Biosciences Inc | Methods for cellularly addressable nucleic acid sequencing |
Family Cites Families (24)
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| US5753433A (en) * | 1909-12-05 | 1998-05-19 | Boehringer Mannheim Gmbh | Method for the sensitive detection of nucleic acids |
| US5753437A (en) | 1987-10-13 | 1998-05-19 | The United States Of America As Represented By The Department Of Health & Human Services | Method of diagnosing cancer susceptibility or metastatic potential |
| US5082830A (en) | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| NL9001639A (nl) | 1990-07-19 | 1992-02-17 | Amc Amsterdam | Pt-houdende verbinding, werkwijze voor de bereiding ervan, alsmede toepassing van dergelijke verbindingen. |
| US5714327A (en) | 1990-07-19 | 1998-02-03 | Kreatech Diagnostics | Platinum-containing compounds, methods for their preparation and applications thereof |
| US5719024A (en) | 1993-08-18 | 1998-02-17 | Applied Spectral Imaging Ltd. | Method for chromosome classification by decorrelation statistical analysis and hardware therefore |
| PT1019420E (pt) | 1995-05-09 | 2003-12-31 | Kreatech Biotech Bv | Metodos para a producao de ligandos baseados em platina para ligacao entre marcadores e moleculas bio-orgaicas na marcacao de moleculas bio-organicas com o fim de detectar substancias biologicas de interesse e conjuntos ("kits") de ensaios de diagnosticos |
| WO1997004133A1 (en) | 1995-07-21 | 1997-02-06 | Regents Of The University Of Minnesota | Analysis of alpha integrins for the diagnosis of diabetic nephropathy |
| BR9712202A (pt) | 1996-10-04 | 1999-08-31 | Novo Nordisk As | Composto, processo para preparar o mesmo, composicão farmacêutica, processo para o tratamento de inflamacão neurogênica em um indivíduo em necessidade deste tratamento, e, uso do composto. |
| US20020197656A1 (en) | 1999-12-17 | 2002-12-26 | Ronghao Li | Cell arrays and the uses thereof |
| US20040115692A1 (en) | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
| US6617148B2 (en) | 2000-06-29 | 2003-09-09 | E. I. Du Pont De Nemours And Company | Natural promoters for gene expression and metabolic monitoring in bacillus species |
| EP1373572B1 (en) | 2001-03-02 | 2006-07-26 | Stratagene California | Compositions and methods using platinum compounds for nucleic acid labeling |
| EP1590661A4 (en) | 2002-11-05 | 2006-07-26 | Roskamp Res Llc | GENE OF PROTEIN-4 (OATPRP4) BITOURETTE SYNDROME AND RELATED DISEASES RELATED TO THE POLYPEPTIDE FOR DENTRANSPORT OF ORGANIC ANIONS |
| US20040197832A1 (en) * | 2003-04-03 | 2004-10-07 | Mor Research Applications Ltd. | Non-invasive prenatal genetic diagnosis using transcervical cells |
| US6995020B2 (en) * | 2003-07-21 | 2006-02-07 | Aureon Laboratories, Inc. | Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization |
| EP1766072B1 (en) | 2004-06-03 | 2010-08-04 | AdvanDx, Inc. | Hybridization of pna probes in alcohol solutions |
| RU2007141067A (ru) * | 2005-04-14 | 2009-05-20 | Мерк Патент ГмбХ (DE) | Терапия с помощью антител к egfr, основанная на повышенном числе копий гена egfr в опухолевых тканях |
| EP2944647A1 (en) * | 2007-07-26 | 2015-11-18 | Cellay, Inc. | Highly visible chromosome-specific probes and related methods |
| US20090036664A1 (en) | 2007-07-31 | 2009-02-05 | Brian Jon Peter | Complex oligonucleotide primer mix |
| WO2009041917A1 (en) * | 2007-09-28 | 2009-04-02 | Agency For Science, Technology And Research | Method of electrically detecting a nucleic acid molecule |
| CN101363048B (zh) * | 2008-08-29 | 2011-11-23 | 芮屈生物技术（上海）有限公司 | 一种nkx2-8基因的原位杂交检测试剂盒及其检测方法和应用 |
| US20130203055A1 (en) | 2010-06-03 | 2013-08-08 | Joan Aurich-Costa | Methods and kits for in situ detection of nucleotide sequences |
| EP2802671B1 (en) | 2011-12-12 | 2018-07-18 | Cellay, Inc. | Methods and kits for room temperature in situ detection of a target nucleic acid in a biological sample |
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2012
- 2012-12-12 EP EP12806823.6A patent/EP2802671B1/en not_active Not-in-force
- 2012-12-12 BR BR112014014307A patent/BR112014014307A2/pt not_active IP Right Cessation
- 2012-12-12 CN CN201510835512.4A patent/CN105385757B/zh not_active Expired - Fee Related
- 2012-12-12 DK DK12806823.6T patent/DK2802671T3/en active
- 2012-12-12 CN CN201280069016.7A patent/CN104145027A/zh active Pending
- 2012-12-12 JP JP2014546191A patent/JP2015505241A/ja active Pending
- 2012-12-12 US US13/712,334 patent/US9145584B2/en not_active Expired - Fee Related
- 2012-12-12 WO PCT/US2012/069178 patent/WO2013090386A2/en not_active Ceased
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2015
- 2015-09-03 US US14/844,823 patent/US10400275B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2013090386A3 (en) | 2013-08-22 |
| CN105385757B (zh) | 2020-04-03 |
| BR112014014307A2 (pt) | 2019-09-24 |
| EP2802671A2 (en) | 2014-11-19 |
| CN104145027A (zh) | 2014-11-12 |
| US9145584B2 (en) | 2015-09-29 |
| JP2015505241A (ja) | 2015-02-19 |
| US20150368701A1 (en) | 2015-12-24 |
| WO2013090386A2 (en) | 2013-06-20 |
| CN105385757A (zh) | 2016-03-09 |
| EP2802671B1 (en) | 2018-07-18 |
| US20130149705A1 (en) | 2013-06-13 |
| US10400275B2 (en) | 2019-09-03 |
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