JP2015205856A - 腸管バリア機能亢進剤、腸疾患治療及び予防用医薬組成物 - Google Patents
腸管バリア機能亢進剤、腸疾患治療及び予防用医薬組成物 Download PDFInfo
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Abstract
Description
さらに本発明は、イソマルトメガロ糖を有効成分として含有する腸疾患治療用医薬組成物に関する。
加えて本発明は、イソマルトメガロ糖を有効成分として含有する腸疾患予防用医薬組成物に関する。
本発明の腸管バリア機能亢進剤、腸疾患治療用医薬組成物及び腸疾患予防用医薬組成物においては、前記イソマルトメガロ糖は直鎖タイプのイソマルトメガロ糖であることが効果の観点で好ましい。
[方法]
7週齢のSprague Dawley系雄性ラットを5日間馴化飼育後、麻酔下で管腔内を洗浄し、15cmの空腸結紮ループを作成した。ループ内にはTJ透過マーカーとしてLucifer yellow(LY)とFITC-dextranを添加した試験液(対照)、これに3-4%(W/V)の環状または直鎖状のイソマルトオリゴ糖ないし、環状または直鎖状のイソマルトメガロ糖を添加した試験液を注入し、20分後放血屠殺した。その後直ちに腸ループ内容液、およびループに残存したマーカーを粘膜とともに回収、LYとFITCの蛍光強度を測定し、それぞれの透過率を求めてTJのバリア機能を評価した。用いた糖の平均重合度は、環状タイプのイソマルトオリゴ糖(Oligo):7.9、イソマルトメガロ糖(Megalo):10.3、直鎖タイプのイソマルトオリゴ糖(Oligo):3.3、イソマルトメガロ糖(Megalo):12.6である。
直鎖と環状のどちらのイソマルトメガロ糖でも、対照群(Control)と比較してLY(図1、図3)、FITC(図2、図4)ともに透過率が低下した。またその作用は、直鎖タイプでより強い傾向が見られた。一方、イソマルトオリゴ糖では、環状タイプLYを除いて対照群との有意差は見られなかった。これらの結果より、イソマルトメガロ糖は空腸上皮のTJを介したバリア機能を高めること、その作用は糖の鎖長に依存することが示唆された。
Claims (6)
- イソマルトメガロ糖を有効成分として含有する腸管バリア機能亢進剤。
- 前記イソマルトメガロ糖が直鎖タイプのイソマルトメガロ糖である請求項1に記載の腸管バリア機能亢進剤。
- イソマルトメガロ糖を有効成分として含有する腸疾患治療用医薬組成物。
- 前記イソマルトメガロ糖が直鎖タイプのイソマルトメガロ糖である請求項3に記載の腸疾患治療用医薬組成物。
- イソマルトメガロ糖を有効成分として含有する腸疾患予防用医薬組成物。
- 前記イソマルトメガロ糖が直鎖タイプのイソマルトメガロ糖である請求項5に記載の腸疾患予防用医薬組成物。
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Cited By (2)
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WO2021019912A1 (ja) | 2019-08-01 | 2021-02-04 | 日本食品化工株式会社 | α-1,6-グルコシル転移反応を触媒する活性を有するタンパク質 |
US11505789B2 (en) | 2018-02-20 | 2022-11-22 | National University Corporation Hokkaido University | Enzyme exhibiting alpha-1,6-glucosyl transfer activity |
Citations (1)
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JP2011178764A (ja) * | 2010-03-04 | 2011-09-15 | Ajinomoto Co Inc | 腸内ビフィズス菌増殖促進剤および腸管バリア機能改善剤 |
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JP2011178764A (ja) * | 2010-03-04 | 2011-09-15 | Ajinomoto Co Inc | 腸内ビフィズス菌増殖促進剤および腸管バリア機能改善剤 |
Non-Patent Citations (6)
Title |
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AMASHEH,M. ET AL: "Quercetin Enhances Epithelial Barrier Function and Increases Claudin-4 Expression in Caco-2 Cells", JOURNAL OF NUTRITION, vol. 138, no. 6, JPN6017043406, 2008, pages 1067 - 1073, ISSN: 0003681260 * |
IWAYA,H. ET AL: "The Delay in the Development of Experimental Colitis from Isomaltosyloligosaccharides in Rats Is Dep", PLOS ONE, vol. 7, no. 11, JPN6017043408, 2012, pages 50658 - 1, ISSN: 0003681262 * |
SUZUKI,T. ET AL: "Role of flavonoids in intestinal tight junction regulation", JOURNAL OF NUTRITIONAL BIOCHEMISTRY, vol. 22, JPN6017043405, 2011, pages 401 - 408, ISSN: 0003681259 * |
岩谷ら: "摂取するイソマルトオリゴ糖/メガロ糖の重合度は実験的大腸炎症状に影響する", 日本農芸化学会大会講演要旨集, vol. 2012, JPN6017043409, 2012, pages 3 - 26, ISSN: 0003681261 * |
篠木ら: "直鎖イソマルトメガロ糖はラット小腸におけるケルセチン配糖体吸収を促進する", 日本農芸化学会大会講演要旨集, vol. 2012, JPN6017043407, 2012, pages 3 - 26, ISSN: 0003681257 * |
鈴木: "消化管タイトジャンクションバリア機能を調整する食品成分", 化学と生物, vol. 49, no. 6, JPN6017043404, 2011, pages 368 - 370, ISSN: 0003681258 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11505789B2 (en) | 2018-02-20 | 2022-11-22 | National University Corporation Hokkaido University | Enzyme exhibiting alpha-1,6-glucosyl transfer activity |
WO2021019912A1 (ja) | 2019-08-01 | 2021-02-04 | 日本食品化工株式会社 | α-1,6-グルコシル転移反応を触媒する活性を有するタンパク質 |
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